AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0139990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 53 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGTGATAATTTGTATTA+TGG 0.130227 1_3:+74060396 None:intergenic
GGTTCCTCCTTCAAGAATTC+TGG 0.172529 1_3:-74059967 Msa0139990:three_prime_UTR
TTCCCTCATGTAGTATTTAA+AGG 0.173459 1_3:+74059666 None:intergenic
TTACAATGATAATGTGTATT+TGG 0.176865 1_3:+74062143 None:intergenic
ATTAAACTTGGAAATGAATT+AGG 0.210390 1_3:-74062943 Msa0139990:intron
TAATTCATTTCCAAGTTTAA+TGG 0.234342 1_3:+74062945 None:intergenic
CAGAATTTGCAGTGCAGCGC+AGG 0.294148 1_3:+74059575 None:intergenic
TAATTATAAATATAATTGTT+AGG 0.307154 1_3:-74060080 Msa0139990:three_prime_UTR
ACAGTGATAATTTGTATTAT+GGG 0.354259 1_3:+74060397 None:intergenic
GACTCTGCATAAGACAATAA+TGG 0.355828 1_3:-74060113 Msa0139990:three_prime_UTR
AGACAATTCCGCAAAGCGTT+TGG 0.379036 1_3:+74060483 None:intergenic
CAAAATGATGCCATTAAACT+TGG 0.402093 1_3:-74062955 Msa0139990:CDS
TAGTACAAAACAGAGGTATA+TGG 0.414342 1_3:+74060157 None:intergenic
CGAAAGAGCCTTGGAGAGAA+AGG 0.423381 1_3:-74059642 Msa0139990:three_prime_UTR
AGAAAGAGCCTTGGAGAGAA+AGG 0.426750 1_3:-74059599 Msa0139990:three_prime_UTR
ACTCTGCATAAGACAATAAT+GGG 0.428670 1_3:-74060112 Msa0139990:three_prime_UTR
GCCTTCATACTGCTTTCTGC+TGG 0.438656 1_3:-74059993 Msa0139990:three_prime_UTR
AATTATAAATATAATTGTTA+GGG 0.440124 1_3:-74060079 Msa0139990:three_prime_UTR
GTTCCTCCTTCAAGAATTCT+GGG 0.442364 1_3:-74059966 Msa0139990:three_prime_UTR
TTGTCTCCCAGAATTCTTGA+AGG 0.447191 1_3:+74059960 None:intergenic
GCTGCTATCCAAACGCTTTG+CGG 0.447265 1_3:-74060491 Msa0139990:CDS
CTCAAGCTTGTTGCATATGT+TGG 0.452344 1_3:-74061208 Msa0139990:CDS
CAACTAAGAGCTTATCTCAT+TGG 0.455962 1_3:+74059843 None:intergenic
CAGGAGAGCACGTGTCTTTG+TGG 0.456160 1_3:-74063010 Msa0139990:CDS
TTGGAGAGAAAGGAAGGGCT+TGG 0.464845 1_3:-74059632 Msa0139990:three_prime_UTR
GGCTACTCTTCCTTCCTGCC+AGG 0.465761 1_3:+74062980 None:intergenic
AAGCCCTTCCTTTCTCTCCA+AGG 0.495149 1_3:+74059634 None:intergenic
TTTGCGGAATTGTCTGATGA+AGG 0.504707 1_3:-74060475 Msa0139990:CDS
CTTCATCTCATAGTTTCTCA+AGG 0.506698 1_3:+74062218 None:intergenic
TGAGGGAAGCGAAAGAGCCT+TGG 0.511561 1_3:-74059651 Msa0139990:three_prime_UTR
CGTTTGGATAGCAGCCAAGC+AGG 0.516208 1_3:+74060499 None:intergenic
CTTGAGAAACTATGAGATGA+AGG 0.518610 1_3:-74062217 Msa0139990:CDS
GCGCAGGGCCTTTCTCTCCA+AGG 0.526608 1_3:+74059591 None:intergenic
TGTCTTTGTGGAAGTAGTCC+TGG 0.529821 1_3:-74062998 Msa0139990:CDS
TTGCCTTTAAATACTACATG+AGG 0.534140 1_3:-74059669 Msa0139990:three_prime_UTR
GCGTGAGAGAGAAAGAGCCT+TGG 0.539498 1_3:-74059608 Msa0139990:three_prime_UTR
AGAGCCTTGGAGAGAAAGGA+AGG 0.541262 1_3:-74059638 Msa0139990:three_prime_UTR
GCCAGCAGAAAGCAGTATGA+AGG 0.568670 1_3:+74059992 None:intergenic
AGAATTTGCAGTGCAGCGCA+GGG 0.576307 1_3:+74059576 None:intergenic
CATACTGCTTTCTGCTGGCG+TGG 0.581915 1_3:-74059988 Msa0139990:three_prime_UTR
TTTGTGGAAGTAGTCCTGGC+AGG 0.584999 1_3:-74062994 Msa0139990:CDS
CAAAATGTGATGGCAAGGCA+AGG 0.585482 1_3:-74059695 Msa0139990:three_prime_UTR
AGAGGCATAAAGTTGCATGA+TGG 0.590712 1_3:-74059934 Msa0139990:three_prime_UTR
TCTCCCAGAATTCTTGAAGG+AGG 0.595273 1_3:+74059963 None:intergenic
TGGAAGTAGTCCTGGCAGGA+AGG 0.600387 1_3:-74062990 Msa0139990:CDS
TGCCTTTAAATACTACATGA+GGG 0.610711 1_3:-74059668 Msa0139990:three_prime_UTR
GCTTGCAAAATGTGATGGCA+AGG 0.615832 1_3:-74059700 Msa0139990:three_prime_UTR
ATCACATCTGCAGATTTGTG+TGG 0.619954 1_3:+74060450 None:intergenic
ACAAATCTGCAGATGTGATG+AGG 0.630753 1_3:-74060446 Msa0139990:CDS
GAGCCTTGGAGAGAAAGGAA+GGG 0.646139 1_3:-74059637 Msa0139990:three_prime_UTR
AGCTGTCAAAGCTACTGATG+AGG 0.663227 1_3:-74062194 Msa0139990:CDS
AATTCTGGGAGACAAAGAAG+AGG 0.676675 1_3:-74059952 Msa0139990:three_prime_UTR
ACATTCTTAGTACAAAACAG+AGG 0.764301 1_3:+74060150 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTTAAATTATTTAAT+TGG - chr1_3:74062139-74062158 Msa0139990:intron 0.0%
!!! ATTAATTTTATTAATTTTAT+TGG - chr1_3:74062506-74062525 Msa0139990:intron 0.0%
!! AGAATATATTGTTAATATTT+AGG - chr1_3:74059760-74059779 Msa0139990:three_prime_UTR 10.0%
!!! ATTGGTAAAATTATAATTTT+TGG - chr1_3:74061331-74061350 Msa0139990:intron 10.0%
!! GAAATAAAATGAAAAAAATT+TGG + chr1_3:74061499-74061518 None:intergenic 10.0%
!!! TTAATATTTAGGCTTTATTT+GGG - chr1_3:74059771-74059790 Msa0139990:three_prime_UTR 15.0%
!!! ATGAATACTTTTGTTTTTTA+CGG - chr1_3:74059817-74059836 Msa0139990:three_prime_UTR 15.0%
!! AAACATAGTTTGAAAAAAAA+GGG + chr1_3:74059980-74059999 None:intergenic 15.0%
!!! TTTTTTTTATCTGTTTTTTG+TGG - chr1_3:74060252-74060271 Msa0139990:three_prime_UTR 15.0%
!! TATTTCAATTGTTAAATGAA+AGG + chr1_3:74060764-74060783 None:intergenic 15.0%
!! TGAAGCAAAAATTAATAAAA+TGG - chr1_3:74060817-74060836 Msa0139990:intron 15.0%
!!! ATTTAATTTAAATATGTGTG+TGG + chr1_3:74062125-74062144 None:intergenic 15.0%
!! ATTGGTAAAATTATAATTTC+TGG - chr1_3:74062547-74062566 Msa0139990:intron 15.0%
!!! TATTATTTTTAACTTGCAAT+TGG - chr1_3:74062877-74062896 Msa0139990:intron 15.0%
!! AGTATGAAATGTTAACTTTA+TGG - chr1_3:74059654-74059673 Msa0139990:three_prime_UTR 20.0%
!!! GTTAATATTTAGGCTTTATT+TGG - chr1_3:74059770-74059789 Msa0139990:three_prime_UTR 20.0%
!!! TAATATTTAGGCTTTATTTG+GGG - chr1_3:74059772-74059791 Msa0139990:three_prime_UTR 20.0%
!!! TGAATACTTTTGTTTTTTAC+GGG - chr1_3:74059818-74059837 Msa0139990:three_prime_UTR 20.0%
!! CAAACATAGTTTGAAAAAAA+AGG + chr1_3:74059981-74060000 None:intergenic 20.0%
!!! TTTGTAACATTTTTTAGCTA+TGG + chr1_3:74060007-74060026 None:intergenic 20.0%
!! AGATAAGATTATAAAAGTTC+AGG - chr1_3:74060112-74060131 Msa0139990:three_prime_UTR 20.0%
!! TAATTCATTTCCAAGTTTAA+TGG + chr1_3:74060199-74060218 None:intergenic 20.0%
!! ATTAAACTTGGAAATGAATT+AGG - chr1_3:74060198-74060217 Msa0139990:three_prime_UTR 20.0%
!!! TTACAATGATAATGTGTATT+TGG + chr1_3:74061001-74061020 None:intergenic 20.0%
!!! TATTATTTTTAGCTTGCAAT+TGG - chr1_3:74061168-74061187 Msa0139990:CDS 20.0%
!!! AAAAATGGGTAATGTTTTTT+CGG - chr1_3:74061376-74061395 Msa0139990:intron 20.0%
!! ATTCAATTGATGAAATGAAA+AGG + chr1_3:74061657-74061676 None:intergenic 20.0%
!! AGAAGTTTGAACAAATAAAT+CGG + chr1_3:74062095-74062114 None:intergenic 20.0%
!! AACATGATTAACCATTATAA+TGG - chr1_3:74062273-74062292 Msa0139990:intron 20.0%
!! TTATATGTAAGGAAAATATG+AGG + chr1_3:74062390-74062409 None:intergenic 20.0%
!! TCATTCAATCATTATATGTA+AGG + chr1_3:74062401-74062420 None:intergenic 20.0%
!!! ACAGTGATAATTTGTATTAT+GGG + chr1_3:74062747-74062766 None:intergenic 20.0%
!!! TACAGTGATAATTTGTATTA+TGG + chr1_3:74062748-74062767 None:intergenic 20.0%
!! GAAAATATGAAGAGTATAAG+AGG + chr1_3:74059590-74059609 None:intergenic 25.0%
! AAATGTTAACTTTATGGACA+TGG - chr1_3:74059660-74059679 Msa0139990:three_prime_UTR 25.0%
! TTCTTACATGTAGTAGTATT+TGG + chr1_3:74059744-74059763 None:intergenic 25.0%
!!! GAATACTTTTGTTTTTTACG+GGG - chr1_3:74059819-74059838 Msa0139990:three_prime_UTR 25.0%
!!! TTTTTTCTTCCCACATTTTA+AGG - chr1_3:74059900-74059919 Msa0139990:three_prime_UTR 25.0%
!! CAAAACTCAAAGTATTAACA+AGG + chr1_3:74060443-74060462 None:intergenic 25.0%
!! AGAATTTGAGTTAAAGTCAA+AGG + chr1_3:74060532-74060551 None:intergenic 25.0%
! TTTGTTACACAAAGTTTCAT+GGG + chr1_3:74060575-74060594 None:intergenic 25.0%
!! AAGTTTTCAAAAGATGGTTA+TGG - chr1_3:74060631-74060650 Msa0139990:intron 25.0%
!! ATAAGATATTAGCTTCAACA+AGG + chr1_3:74060860-74060879 None:intergenic 25.0%
! TTGTTGAAGCTAATATCTTA+TGG - chr1_3:74060859-74060878 Msa0139990:intron 25.0%
! TACTAGGAGAGTTTATTATA+AGG + chr1_3:74061234-74061253 None:intergenic 25.0%
!!! TAATTTTTGGAGACATCTTA+TGG - chr1_3:74061344-74061363 Msa0139990:intron 25.0%
!!! TTATGGATTAGACAGAAAAA+TGG - chr1_3:74061361-74061380 Msa0139990:intron 25.0%
!!! TATGGATTAGACAGAAAAAT+GGG - chr1_3:74061362-74061381 Msa0139990:intron 25.0%
!! AAAAATTTGGATACCCATTT+TGG + chr1_3:74061486-74061505 None:intergenic 25.0%
! TATTCATCGTTATCAAAAGA+AGG + chr1_3:74061540-74061559 None:intergenic 25.0%
!!! TTAATACATTGTTTTGCCTT+TGG - chr1_3:74061790-74061809 Msa0139990:intron 25.0%
! AGAAAATAAACTCAAAGTGA+AGG + chr1_3:74061845-74061864 None:intergenic 25.0%
!!! AAGTTTTCAAATGTTGGTTA+TGG - chr1_3:74062218-74062237 Msa0139990:CDS 25.0%
!! TACTATGAGAGTTTTCTATA+AGG + chr1_3:74062328-74062347 None:intergenic 25.0%
! CAATTGTAGAGTATGAAAAA+TGG - chr1_3:74063350-74063369 Msa0139990:intron 25.0%
!!! GTAAAAAGAAGCATAGATTT+TGG - chr1_3:74063419-74063438 Msa0139990:intron 25.0%
TTAACTTTATGGACATGGTA+AGG - chr1_3:74059665-74059684 Msa0139990:three_prime_UTR 30.0%
TAACTTTATGGACATGGTAA+GGG - chr1_3:74059666-74059685 Msa0139990:three_prime_UTR 30.0%
TTACATGTAGTAGTATTTGG+TGG + chr1_3:74059741-74059760 None:intergenic 30.0%
AAATACAGCTATAGCAGAAA+AGG + chr1_3:74059926-74059945 None:intergenic 30.0%
AGACGATTTACAAATAGTCT+TGG + chr1_3:74060036-74060055 None:intergenic 30.0%
! ATCGTCTTTTGTGAAACTTT+TGG - chr1_3:74060049-74060068 Msa0139990:three_prime_UTR 30.0%
! GTTTAATGGCATCATTTTGA+TGG + chr1_3:74060185-74060204 None:intergenic 30.0%
CAAAATGATGCCATTAAACT+TGG - chr1_3:74060186-74060205 Msa0139990:three_prime_UTR 30.0%
!!! TTATCTGTTTTTTGTGGAAG+AGG - chr1_3:74060258-74060277 Msa0139990:three_prime_UTR 30.0%
TTCAGAAGCAATGTTGTAAA+GGG + chr1_3:74060495-74060514 None:intergenic 30.0%
ATTCAGAAGCAATGTTGTAA+AGG + chr1_3:74060496-74060515 None:intergenic 30.0%
! GTTTGTTACACAAAGTTTCA+TGG + chr1_3:74060576-74060595 None:intergenic 30.0%
!! CCATCTTTTGAAAACTTTTG+TGG + chr1_3:74060628-74060647 None:intergenic 30.0%
! CCACAAAAGTTTTCAAAAGA+TGG - chr1_3:74060625-74060644 Msa0139990:intron 30.0%
TTTAATCTACACAAATCTGC+AGG - chr1_3:74060895-74060914 Msa0139990:intron 30.0%
AATGAGAAAGAACACAAAAG+TGG + chr1_3:74061099-74061118 None:intergenic 30.0%
!!! TTTTAGCTTGCAATTGGTTA+TGG - chr1_3:74061174-74061193 Msa0139990:CDS 30.0%
TCTTACAATAGACACATGTT+TGG - chr1_3:74061304-74061323 Msa0139990:intron 30.0%
! TAACGATGAATACCTTTTTC+CGG - chr1_3:74061548-74061567 Msa0139990:intron 30.0%
CAGTTATGAAAAAGGAATAC+CGG + chr1_3:74061570-74061589 None:intergenic 30.0%
CACAAATTCCACATAATCAA+GGG + chr1_3:74061714-74061733 None:intergenic 30.0%
TAAAAGCAATACAAAGCCAA+AGG + chr1_3:74061809-74061828 None:intergenic 30.0%
AGTGTTGTAAAGGGAAAATT+TGG + chr1_3:74062027-74062046 None:intergenic 30.0%
TTTGGAAGTAGTGTTGTAAA+GGG + chr1_3:74062036-74062055 None:intergenic 30.0%
ATTTGGAAGTAGTGTTGTAA+AGG + chr1_3:74062037-74062056 None:intergenic 30.0%
TACAACACTACTTCCAAATT+TGG - chr1_3:74062038-74062057 Msa0139990:intron 30.0%
AAGGAAACAACAACCAAATT+TGG + chr1_3:74062054-74062073 None:intergenic 30.0%
!! TAAATCGGATTTTGAGTCAT+AGG + chr1_3:74062080-74062099 None:intergenic 30.0%
! GAAAACTTTTGAGCATATGT+TGG + chr1_3:74062206-74062225 None:intergenic 30.0%
! GCTCAAAAGTTTTCAAATGT+TGG - chr1_3:74062212-74062231 Msa0139990:CDS 30.0%
AGGTATGGCAAAAGAAAAAT+TGG + chr1_3:74062308-74062327 None:intergenic 30.0%
! TGAGAGTTTTCTATAAGGTA+TGG + chr1_3:74062323-74062342 None:intergenic 30.0%
GAAAATATGAGGAATCCAAA+AGG + chr1_3:74062379-74062398 None:intergenic 30.0%
AGACACATGTTTAGTAACAT+TGG - chr1_3:74062529-74062548 Msa0139990:intron 30.0%
!! TTGATTGTTTTGTGCAGAAT+AGG - chr1_3:74062903-74062922 Msa0139990:intron 30.0%
!! TGATTGTTTTGTGCAGAATA+GGG - chr1_3:74062904-74062923 Msa0139990:intron 30.0%
! TAGTACAAAACAGAGGTATA+TGG + chr1_3:74062987-74063006 None:intergenic 30.0%
ACATTCTTAGTACAAAACAG+AGG + chr1_3:74062994-74063013 None:intergenic 30.0%
ACTCTGCATAAGACAATAAT+GGG - chr1_3:74063029-74063048 Msa0139990:intron 30.0%
TTGCCTTTAAATACTACATG+AGG - chr1_3:74063472-74063491 Msa0139990:intron 30.0%
TGCCTTTAAATACTACATGA+GGG - chr1_3:74063473-74063492 Msa0139990:intron 30.0%
TTCCCTCATGTAGTATTTAA+AGG + chr1_3:74063478-74063497 None:intergenic 30.0%
CATTTCATACTGTTCGTTTC+AGG + chr1_3:74059645-74059664 None:intergenic 35.0%
GGATATGCAAGTGAATAAGA+TGG - chr1_3:74059701-74059720 Msa0139990:three_prime_UTR 35.0%
!! ATTTAGGCTTTATTTGGGGA+AGG - chr1_3:74059776-74059795 Msa0139990:three_prime_UTR 35.0%
!!! ACTTTTGTTTTTTACGGGGA+AGG - chr1_3:74059823-74059842 Msa0139990:three_prime_UTR 35.0%
CTTTATATTCCTGACCTACA+CGG - chr1_3:74059867-74059886 Msa0139990:three_prime_UTR 35.0%
CAGAAAAGGCCTTAAAATGT+GGG + chr1_3:74059912-74059931 None:intergenic 35.0%
AATTCGTTGCAAACTTCAGT+TGG - chr1_3:74060323-74060342 Msa0139990:three_prime_UTR 35.0%
ATTCGTTGCAAACTTCAGTT+GGG - chr1_3:74060324-74060343 Msa0139990:three_prime_UTR 35.0%
TACAACATTGCTTCTGAATC+TGG - chr1_3:74060497-74060516 Msa0139990:CDS 35.0%
GACTAAGAGAGTCGATTAAA+AGG + chr1_3:74060691-74060710 None:intergenic 35.0%
CTTCATCTCATAGTTTCTCA+AGG + chr1_3:74060926-74060945 None:intergenic 35.0%
CTTGAGAAACTATGAGATGA+AGG - chr1_3:74060924-74060943 Msa0139990:intron 35.0%
AGAAAGAACACAAAAGTGGA+AGG + chr1_3:74061095-74061114 None:intergenic 35.0%
! TTCACGTTCACACTTTTACT+AGG + chr1_3:74061250-74061269 None:intergenic 35.0%
AAAAATATGCAGAGTCCAAG+AGG + chr1_3:74061285-74061304 None:intergenic 35.0%
! AGACACATGTTTGGTAACAT+TGG - chr1_3:74061313-74061332 Msa0139990:intron 35.0%
!!! ATTTTGGTTCGATTCTTGCA+TGG + chr1_3:74061470-74061489 None:intergenic 35.0%
ATGCAAGAATCGAACCAAAA+TGG - chr1_3:74061469-74061488 Msa0139990:intron 35.0%
TGCAAGAATCGAACCAAAAT+GGG - chr1_3:74061470-74061489 Msa0139990:intron 35.0%
GAAAAAGGAATACCGGAAAA+AGG + chr1_3:74061563-74061582 None:intergenic 35.0%
CTATGCAGCAGTTATGAAAA+AGG + chr1_3:74061578-74061597 None:intergenic 35.0%
CTTAATCATTATGATGCCCT+TGG + chr1_3:74061622-74061641 None:intergenic 35.0%
ACGTTATTCCCTTGATTATG+TGG - chr1_3:74061703-74061722 Msa0139990:intron 35.0%
CCACAAATTCCACATAATCA+AGG + chr1_3:74061715-74061734 None:intergenic 35.0%
CCTTGATTATGTGGAATTTG+TGG - chr1_3:74061712-74061731 Msa0139990:intron 35.0%
! TTTCTTTTGATCAGGTGGTT+AGG - chr1_3:74061909-74061928 Msa0139990:intron 35.0%
TGTTAGTGTTGCAAAGTTAG+AGG - chr1_3:74061999-74062018 Msa0139990:intron 35.0%
!! GATTTTGAGTCATAGGTCAA+AGG + chr1_3:74062073-74062092 None:intergenic 35.0%
! TAATTTCTGGAGACGTCTTA+TGG - chr1_3:74062560-74062579 Msa0139990:intron 35.0%
GACTCTGCATAAGACAATAA+TGG - chr1_3:74063028-74063047 Msa0139990:intron 35.0%
CAACTAAGAGCTTATCTCAT+TGG + chr1_3:74063301-74063320 None:intergenic 35.0%
!!! TTTTGGCTTGCAAAATGTGA+TGG - chr1_3:74063436-74063455 Msa0139990:intron 35.0%
GAGGAAAGAGAACTTACAGA+AGG + chr1_3:74059571-74059590 None:intergenic 40.0%
GACATGGTAAGGGTTAGAAA+AGG - chr1_3:74059676-74059695 Msa0139990:three_prime_UTR 40.0%
TGGTAAGGGTTAGAAAAGGA+TGG - chr1_3:74059680-74059699 Msa0139990:three_prime_UTR 40.0%
!! GCTTTATTTGGGGAAGGTAA+GGG - chr1_3:74059782-74059801 Msa0139990:three_prime_UTR 40.0%
GGGAAGGTTGAACTTGATAT+GGG - chr1_3:74059839-74059858 Msa0139990:three_prime_UTR 40.0%
GGAAGGTTGAACTTGATATG+GGG - chr1_3:74059840-74059859 Msa0139990:three_prime_UTR 40.0%
AAAACAAAGAGCTACCGTGT+AGG + chr1_3:74059884-74059903 None:intergenic 40.0%
GCAGAAAAGGCCTTAAAATG+TGG + chr1_3:74059913-74059932 None:intergenic 40.0%
GCCATATCTGACATACAATC+TGG - chr1_3:74059954-74059973 Msa0139990:three_prime_UTR 40.0%
GCCAGATTGTATGTCAGATA+TGG + chr1_3:74059958-74059977 None:intergenic 40.0%
! GAAACTTTTGGCATGTTTGC+AGG - chr1_3:74060061-74060080 Msa0139990:three_prime_UTR 40.0%
!!! TCTGTTTTTTGTGGAAGAGG+TGG - chr1_3:74060261-74060280 Msa0139990:three_prime_UTR 40.0%
! TTTTGTGGAAGAGGTGGAAA+TGG - chr1_3:74060267-74060286 Msa0139990:three_prime_UTR 40.0%
TTCGTTGCAAACTTCAGTTG+GGG - chr1_3:74060325-74060344 Msa0139990:three_prime_UTR 40.0%
! TTGATGCATCGTTTTGCCTT+TGG - chr1_3:74060379-74060398 Msa0139990:CDS 40.0%
GAGCAGAAACACAAATCCAA+AGG + chr1_3:74060398-74060417 None:intergenic 40.0%
!! AAGGGATAATTTGCTACCTC+TGG + chr1_3:74060477-74060496 None:intergenic 40.0%
AGAGAGTCGATTAAAAGGCA+TGG + chr1_3:74060686-74060705 None:intergenic 40.0%
GAATTCCATTGCATGCTTCT+AGG - chr1_3:74060724-74060743 Msa0139990:intron 40.0%
GAATTCCATTGTATGCCTCT+TGG - chr1_3:74061267-74061286 Msa0139990:intron 40.0%
! CTGCTGCATAGATCTTTTTG+TGG - chr1_3:74061587-74061606 Msa0139990:intron 40.0%
! TTTTGTGGCAGCTAAATCCA+AGG - chr1_3:74061602-74061621 Msa0139990:intron 40.0%
TTTGTGGCAGCTAAATCCAA+GGG - chr1_3:74061603-74061622 Msa0139990:intron 40.0%
TCTCGCAACAGTATAAGAGA+CGG - chr1_3:74061882-74061901 Msa0139990:intron 40.0%
!! CTCAAGCTTGTTGCATATGT+TGG - chr1_3:74061933-74061952 Msa0139990:intron 40.0%
GGCTTGCAAGTCCATTATAA+TGG + chr1_3:74062287-74062306 None:intergenic 40.0%
! GAATTCCATTGTACGCCTTT+TGG - chr1_3:74062361-74062380 Msa0139990:intron 40.0%
! TTTTTGTCGACATACCTGCT+TGG - chr1_3:74062628-74062647 Msa0139990:intron 40.0%
TTTGCGGAATTGTCTGATGA+AGG - chr1_3:74062666-74062685 Msa0139990:intron 40.0%
ATCACATCTGCAGATTTGTG+TGG + chr1_3:74062694-74062713 None:intergenic 40.0%
ACAAATCTGCAGATGTGATG+AGG - chr1_3:74062695-74062714 Msa0139990:intron 40.0%
GTTCCTCCTTCAAGAATTCT+GGG - chr1_3:74063175-74063194 Msa0139990:intron 40.0%
TTGTCTCCCAGAATTCTTGA+AGG + chr1_3:74063184-74063203 None:intergenic 40.0%
! AATTCTGGGAGACAAAGAAG+AGG - chr1_3:74063189-74063208 Msa0139990:intron 40.0%
! AGAGGCATAAAGTTGCATGA+TGG - chr1_3:74063207-74063226 Msa0139990:intron 40.0%
!! GGCTTTATTTGGGGAAGGTA+AGG - chr1_3:74059781-74059800 Msa0139990:three_prime_UTR 45.0%
GGGGAAGGTTGAACTTGATA+TGG - chr1_3:74059838-74059857 Msa0139990:three_prime_UTR 45.0%
GGTTGAACTTGATATGGGGA+AGG - chr1_3:74059844-74059863 Msa0139990:three_prime_UTR 45.0%
TGTCTTTGTGGAAGTAGTCC+TGG - chr1_3:74060143-74060162 Msa0139990:three_prime_UTR 45.0%
TCGTTGCAAACTTCAGTTGG+GGG - chr1_3:74060326-74060345 Msa0139990:three_prime_UTR 45.0%
! TTTTGCTAGTGCAAAGCCAG+AGG - chr1_3:74060458-74060477 Msa0139990:CDS 45.0%
! CAAGTCCTAGAAGCATGCAA+TGG + chr1_3:74060732-74060751 None:intergenic 45.0%
AGCTGTCAAAGCTACTGATG+AGG - chr1_3:74060947-74060966 Msa0139990:intron 45.0%
! AGAGTCCAAGAGGCATACAA+TGG + chr1_3:74061275-74061294 None:intergenic 45.0%
!!! ACGGCTGGTTTCTTTTGATC+AGG - chr1_3:74061901-74061920 Msa0139990:intron 45.0%
!! GCTGGTTTCTTTTGATCAGG+TGG - chr1_3:74061904-74061923 Msa0139990:intron 45.0%
! GGAATCCAAAAGGCGTACAA+TGG + chr1_3:74062369-74062388 None:intergenic 45.0%
! AGACAATTCCGCAAAGCGTT+TGG + chr1_3:74062661-74062680 None:intergenic 45.0%
GGTTCCTCCTTCAAGAATTC+TGG - chr1_3:74063174-74063193 Msa0139990:intron 45.0%
TCTCCCAGAATTCTTGAAGG+AGG + chr1_3:74063181-74063200 None:intergenic 45.0%
GCTTGCAAAATGTGATGGCA+AGG - chr1_3:74063441-74063460 Msa0139990:intron 45.0%
CAAAATGTGATGGCAAGGCA+AGG - chr1_3:74063446-74063465 Msa0139990:intron 45.0%
AGAAAGAGCCTTGGAGAGAA+AGG - chr1_3:74063542-74063561 Msa0139990:intron 45.0%
!!! AAGATTTTTTTTTTTAAAAA+GGG + chr1_3:74060358-74060377 None:intergenic 5.0%
!!! TAAGATTTTTTTTTTTAAAA+AGG + chr1_3:74060359-74060378 None:intergenic 5.0%
!! AATAATAACAATAATAATAT+AGG - chr1_3:74062429-74062448 Msa0139990:intron 5.0%
!! TAATTATAAATATAATTGTT+AGG - chr1_3:74063061-74063080 Msa0139990:intron 5.0%
!! AATTATAAATATAATTGTTA+GGG - chr1_3:74063062-74063081 Msa0139990:intron 5.0%
AAAGAGCTACCGTGTAGGTC+AGG + chr1_3:74059879-74059898 None:intergenic 50.0%
TTTGTGGAAGTAGTCCTGGC+AGG - chr1_3:74060147-74060166 Msa0139990:three_prime_UTR 50.0%
CGTTGCAAACTTCAGTTGGG+GGG - chr1_3:74060327-74060346 Msa0139990:three_prime_UTR 50.0%
GCAACAGTATAAGAGACGGC+TGG - chr1_3:74061886-74061905 Msa0139990:intron 50.0%
GCTGCTATCCAAACGCTTTG+CGG - chr1_3:74062650-74062669 Msa0139990:intron 50.0%
GCCTTCATACTGCTTTCTGC+TGG - chr1_3:74063148-74063167 Msa0139990:intron 50.0%
GCCAGCAGAAAGCAGTATGA+AGG + chr1_3:74063152-74063171 None:intergenic 50.0%
CGAAAGAGCCTTGGAGAGAA+AGG - chr1_3:74063499-74063518 Msa0139990:intron 50.0%
AGAGCCTTGGAGAGAAAGGA+AGG - chr1_3:74063503-74063522 Msa0139990:intron 50.0%
GAGCCTTGGAGAGAAAGGAA+GGG - chr1_3:74063504-74063523 Msa0139990:intron 50.0%
AAGCCCTTCCTTTCTCTCCA+AGG + chr1_3:74063510-74063529 None:intergenic 50.0%
TTGGAGAGAAAGGAAGGGCT+TGG - chr1_3:74063509-74063528 Msa0139990:intron 50.0%
AGAATTTGCAGTGCAGCGCA+GGG + chr1_3:74063568-74063587 None:intergenic 50.0%
! CAGGAGAGCACGTGTCTTTG+TGG - chr1_3:74060131-74060150 Msa0139990:three_prime_UTR 55.0%
TGGAAGTAGTCCTGGCAGGA+AGG - chr1_3:74060151-74060170 Msa0139990:three_prime_UTR 55.0%
CGTTTGGATAGCAGCCAAGC+AGG + chr1_3:74062645-74062664 None:intergenic 55.0%
CATACTGCTTTCTGCTGGCG+TGG - chr1_3:74063153-74063172 Msa0139990:intron 55.0%
TGAGGGAAGCGAAAGAGCCT+TGG - chr1_3:74063490-74063509 Msa0139990:intron 55.0%
GCGTGAGAGAGAAAGAGCCT+TGG - chr1_3:74063533-74063552 Msa0139990:intron 55.0%
CAGAATTTGCAGTGCAGCGC+AGG + chr1_3:74063569-74063588 None:intergenic 55.0%
GGCTACTCTTCCTTCCTGCC+AGG + chr1_3:74060164-74060183 None:intergenic 60.0%
GCGCAGGGCCTTTCTCTCCA+AGG + chr1_3:74063553-74063572 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 74059563 74063600 74059563 ID=Msa0139990;Name=Msa0139990
chr1_3 mRNA 74059563 74063600 74059563 ID=Msa0139990-mRNA-1;Parent=Msa0139990;Name=Msa0139990-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0.5|0.4|0.8|0.25|0.4|5|806|148
chr1_3 exon 74059563 74060541 74059563 ID=Msa0139990-mRNA-1:exon:25714;Parent=Msa0139990-mRNA-1
chr1_3 exon 74061164 74061240 74061164 ID=Msa0139990-mRNA-1:exon:25713;Parent=Msa0139990-mRNA-1
chr1_3 exon 74062147 74062246 74062147 ID=Msa0139990-mRNA-1:exon:25712;Parent=Msa0139990-mRNA-1
chr1_3 exon 74062944 74063029 74062944 ID=Msa0139990-mRNA-1:exon:25711;Parent=Msa0139990-mRNA-1
chr1_3 exon 74063590 74063600 74063590 ID=Msa0139990-mRNA-1:exon:25710;Parent=Msa0139990-mRNA-1
chr1_3 CDS 74063590 74063600 74063590 ID=Msa0139990-mRNA-1:cds;Parent=Msa0139990-mRNA-1
chr1_3 CDS 74062944 74063029 74062944 ID=Msa0139990-mRNA-1:cds;Parent=Msa0139990-mRNA-1
chr1_3 CDS 74062147 74062246 74062147 ID=Msa0139990-mRNA-1:cds;Parent=Msa0139990-mRNA-1
chr1_3 CDS 74061164 74061240 74061164 ID=Msa0139990-mRNA-1:cds;Parent=Msa0139990-mRNA-1
chr1_3 CDS 74060369 74060541 74060369 ID=Msa0139990-mRNA-1:cds;Parent=Msa0139990-mRNA-1
chr1_3 three_prime_UTR 74059563 74060368 74059563 ID=Msa0139990-mRNA-1:three_prime_utr;Parent=Msa0139990-mRNA-1
Gene Sequence

>Msa0139990

ATGGACCTTCTGAGAGCACGTGTCTTTGTGGAAGTAGTCCTGGCAGGAAGGAAGAGTAGCCATCAAAATGATGCCATTAAACTTGGAAATGAATTAGGTGACACCTTGAGAAACTATGAGATGAAGGAGCTGTCAAAGCTACTGATGAGGACTTGTTTACATGCTTGCTTGCTGCTGTCCAAATACACATTATCATTGTGGTTAGGACTCAAGCTTGTTGCATATGTTGGTATAGTTGATGCTTCAAATGAAGAAAAGCACAATTTTGAGCTTTATCATATTTTTGTCGACATACCTGCTTGGCTGCTATCCAAACGCTTTGCGGAATTGTCTGATGAAGGATCCACACAAATCTGCAGATGTGATGAGGACTTGTTACATGCTTGTTTGTTGCTGCCCATAATACAAATTATCACTGTAAGTAAAAAAATGAAAACAATTGTGTAG
Protein sequence

>Msa0139990

MDLLRARVFVEVVLAGRKSSHQNDAIKLGNELGDTLRNYEMKELSKLLMRTCLHACLLLSKYTLSLWLGLKLVAYVGIVDASNEEKHNFELYHIFVDIPAWLLSKRFAELSDEGSTQICRCDEDLLHACLLLPIIQIITVSKKMKTIV*