AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0905970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0905970 MtrunA17_Chr6g0453791 93.714 175 11 0 1 175 1 175 7.14e-121 338
Msa0905970 MtrunA17_Chr5g0440711 63.810 105 37 1 34 137 33 137 3.86e-45 145
Msa0905970 MtrunA17_Chr3g0144921 56.780 118 50 1 28 144 27 144 2.85e-44 144
Msa0905970 MtrunA17_Chr5g0440804 51.200 125 59 2 31 153 29 153 1.39e-42 139
Msa0905970 MtrunA17_Chr6g0453921 42.331 163 89 3 9 167 17 178 1.26e-33 117
Msa0905970 MtrunA17_Chr5g0440791 52.688 93 43 1 62 153 16 108 1.63e-30 107
Msa0905970 MtrunA17_Chr6g0453801 44.186 129 65 2 6 133 16 138 1.82e-28 103
Msa0905970 MtrunA17_Chr4g0001571 32.168 143 84 3 31 161 29 170 5.24e-22 87.4
Msa0905970 MtrunA17_Chr6g0481491 36.667 120 71 4 13 131 12 127 3.04e-14 67.8
Msa0905970 MtrunA17_Chr2g0323021 36.441 118 66 6 31 141 25 140 1.02e-13 66.6
Msa0905970 MtrunA17_Chr6g0459441 37.113 97 59 2 31 126 26 121 4.00e-13 64.3
Msa0905970 MtrunA17_Chr7g0253701 33.613 119 68 4 1 116 1 111 2.22e-12 62.0
Msa0905970 MtrunA17_Chr7g0253671 33.613 119 68 4 1 116 1 111 2.22e-12 62.0
Msa0905970 MtrunA17_Chr7g0253681 33.613 119 69 4 1 116 1 112 7.69e-12 60.5
Msa0905970 MtrunA17_Chr1g0211101 27.778 126 88 2 4 128 8 131 2.26e-11 59.7
Msa0905970 MtrunA17_Chr3g0132701 33.898 118 72 4 16 128 8 124 2.35e-11 60.1
Msa0905970 MtrunA17_Chr1g0205701 30.000 100 66 3 30 126 24 122 4.24e-11 58.9
Msa0905970 MtrunA17_Chr3g0125791 31.915 94 61 2 31 124 33 123 8.22e-11 57.0
Msa0905970 MtrunA17_Chr3g0132511 33.051 118 73 4 16 128 8 124 9.48e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0905970 AT2G27035.1 42.857 133 71 2 30 161 27 155 4.26e-36 123
Msa0905970 AT5G15350.1 43.949 157 82 3 7 162 6 157 1.20e-35 122
Msa0905970 AT4G12880.1 47.967 123 62 2 6 127 5 126 3.30e-31 110
Msa0905970 AT3G01070.1 46.078 102 53 2 29 128 25 126 1.59e-19 81.3
Msa0905970 AT3G27200.1 34.146 123 75 3 5 126 4 121 1.55e-15 70.9
Msa0905970 AT2G32300.1 29.710 138 95 2 31 167 26 162 4.04e-15 71.2
Msa0905970 AT4G12880.2 52.174 69 32 1 60 127 20 88 4.85e-15 67.8
Msa0905970 AT2G32300.2 29.710 138 95 2 31 167 56 192 8.21e-15 70.9
Msa0905970 AT3G17675.1 31.034 116 78 2 12 126 13 127 4.58e-13 63.2
Msa0905970 AT2G31050.1 36.975 119 63 5 27 136 25 140 9.54e-13 63.9
Msa0905970 AT2G26720.1 35.849 106 64 4 23 126 22 125 3.28e-12 62.8
Msa0905970 AT5G26330.1 26.061 165 92 5 9 144 1 164 4.49e-12 62.0
Msa0905970 AT2G23990.1 35.000 120 70 5 25 137 21 139 2.78e-11 60.1
Msa0905970 AT1G79800.1 30.714 140 77 7 16 139 15 150 5.34e-11 58.9
Msa0905970 AT2G25060.1 34.211 114 64 5 33 136 33 145 8.70e-11 58.2

Find 49 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGATGGTGGTTCTATTT+TGG 0.227424 6_2:-4430989 Msa0905970:CDS
TTTGATGGTGAGTGTGGTTT+TGG 0.267985 6_2:-4432676 Msa0905970:CDS
GACATTCTCTTTCCAACCTT+TGG 0.300339 6_2:+4432590 None:intergenic
GCATCAATGTATACATGCTT+TGG 0.302107 6_2:-4430967 Msa0905970:CDS
CATCAATGTATACATGCTTT+GGG 0.359484 6_2:-4430966 Msa0905970:CDS
GGTGGAGGTTACTGCTTTCA+TGG 0.363773 6_2:-4431084 Msa0905970:CDS
ATATACACTTGTTCTATTGA+AGG 0.384245 6_2:-4432780 Msa0905970:five_prime_UTR
CCTCCCACCTTATGAAGCTT+TGG 0.418516 6_2:+4432615 None:intergenic
TAATGTGGTGTATGTGTGCT+TGG 0.433588 6_2:-4430932 Msa0905970:CDS
ATATGTGTAAAGTGTGAAGT+AGG 0.434791 6_2:+4432805 None:intergenic
TCTTGTCAAGTTTCTTATGA+AGG 0.439117 6_2:+4431161 None:intergenic
TATGTGTAAAGTGTGAAGTA+GGG 0.445945 6_2:+4432806 None:intergenic
ATGGAGATGGTGCAGGTGAT+TGG 0.459673 6_2:+4431031 None:intergenic
ATTGTGGCCAATGGTGGTCA+TGG 0.472414 6_2:-4432643 Msa0905970:CDS
AGCATAGAAAGAATTGTGTT+AGG 0.477691 6_2:-4432755 Msa0905970:five_prime_UTR
GGACCTCCCATGACCACCAT+TGG 0.487403 6_2:+4432636 None:intergenic
TGGGATTATTGTTGCTAATG+TGG 0.493795 6_2:-4430947 Msa0905970:CDS
TTGTGGCCAATGGTGGTCAT+GGG 0.494982 6_2:-4432642 Msa0905970:CDS
TCGCGATGTGGTTCAATTGA+CGG 0.504641 6_2:-4431133 Msa0905970:CDS
TACTACTTTATCACCGGTGG+AGG 0.525846 6_2:-4431099 Msa0905970:CDS
CAAAAGATACTACAACGTCT+TGG 0.534059 6_2:-4431223 Msa0905970:CDS
TTCATTGTATCTGAAAGAGA+TGG 0.538095 6_2:+4431012 None:intergenic
GGGAGGTCCAAAGCTTCATA+AGG 0.541271 6_2:-4432622 Msa0905970:CDS
AATGTTGTCATTGTGGCCAA+TGG 0.551433 6_2:-4432652 Msa0905970:CDS
TCTCAAGAGCATGTCTATGT+TGG 0.570965 6_2:-4432549 Msa0905970:CDS
TGGCCAATGGTGGTCATGGG+AGG 0.572145 6_2:-4432639 Msa0905970:CDS
TTGGTGTTCTTGAACGTCCA+CGG 0.573043 6_2:+4431050 None:intergenic
AGCATGTCTATGTTGGTGAT+TGG 0.573669 6_2:-4432542 Msa0905970:CDS
CATAAGGTGGGAGGATCCAA+AGG 0.579599 6_2:-4432606 Msa0905970:CDS
TCAGATACAATGAAATCTGA+TGG 0.580013 6_2:-4431003 Msa0905970:CDS
GTCTTGGAAGTGAACAAGAC+AGG 0.585983 6_2:-4431207 Msa0905970:CDS
AGAATGTCAACTACACTACA+TGG 0.589276 6_2:-4432575 Msa0905970:CDS
ATAAGAAACTTGACAAGAGG+TGG 0.589525 6_2:-4431156 Msa0905970:CDS
ATGAAAGCAGTAACCTCCAC+CGG 0.596550 6_2:+4431086 None:intergenic
TTCATAAGAAACTTGACAAG+AGG 0.597109 6_2:-4431159 Msa0905970:CDS
ATGTGTAAAGTGTGAAGTAG+GGG 0.598258 6_2:+4432807 None:intergenic
AGGTCCAAAGCTTCATAAGG+TGG 0.599406 6_2:-4432619 Msa0905970:CDS
GAAAGAGATGGAGATGGTGC+AGG 0.601797 6_2:+4431024 None:intergenic
AGGTGGGAGGATCCAAAGGT+TGG 0.605617 6_2:-4432602 Msa0905970:CDS
AAGACATACTACTTTATCAC+CGG 0.609394 6_2:-4431105 Msa0905970:CDS
ACATACTACTTTATCACCGG+TGG 0.610826 6_2:-4431102 Msa0905970:CDS
GTTGTCATTGTGGCCAATGG+TGG 0.618802 6_2:-4432649 Msa0905970:CDS
TCATGGAATGAAAGTTGCCG+TGG 0.625731 6_2:-4431067 Msa0905970:CDS
GACAAGAGGTGGTCGCGATG+TGG 0.637295 6_2:-4431145 Msa0905970:CDS
GGTCCAAAGCTTCATAAGGT+GGG 0.645031 6_2:-4432618 Msa0905970:CDS
CCAAAGCTTCATAAGGTGGG+AGG 0.659873 6_2:-4432615 Msa0905970:CDS
GATACAATGAAATCTGATGG+TGG 0.678488 6_2:-4431000 Msa0905970:CDS
CGATGTGGTTCAATTGACGG+AGG 0.705107 6_2:-4431130 Msa0905970:CDS
GTATCTGAAAGAGATGGAGA+TGG 0.708743 6_2:+4431018 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATGTTAAAGTTTAAAAA+CGG + chr6_2:4431719-4431738 None:intergenic 10.0%
!! ATGTTTAACAAAATAATTAT+TGG - chr6_2:4432349-4432368 Msa0905970:intron 10.0%
!! TAAAAAATTGACAATTATTT+GGG - chr6_2:4432741-4432760 Msa0905970:five_prime_UTR 10.0%
!! ATAATGTTAAAGTTTAAAAA+CGG + chr6_2:4431719-4431738 None:intergenic 10.0%
!! ATGTTTAACAAAATAATTAT+TGG - chr6_2:4432349-4432368 Msa0905970:intron 10.0%
!! TAAAAAATTGACAATTATTT+GGG - chr6_2:4432741-4432760 Msa0905970:five_prime_UTR 10.0%
!!! ATATGAACTTTGATAATTTT+AGG - chr6_2:4431411-4431430 Msa0905970:intron 15.0%
!!! CTTTTGTTTTTACAAATTTT+GGG + chr6_2:4431574-4431593 None:intergenic 15.0%
!! ACAAATCAATAAATGAAAAA+AGG + chr6_2:4431668-4431687 None:intergenic 15.0%
!!! TCTCTTTTTTTACAAAAAAA+AGG - chr6_2:4431763-4431782 Msa0905970:intron 15.0%
!!! TAACAAAATAATTATTGGTT+TGG - chr6_2:4432354-4432373 Msa0905970:intron 15.0%
!! GTAAAAAATTGACAATTATT+TGG - chr6_2:4432740-4432759 Msa0905970:five_prime_UTR 15.0%
!!! ATATGAACTTTGATAATTTT+AGG - chr6_2:4431411-4431430 Msa0905970:intron 15.0%
!!! CTTTTGTTTTTACAAATTTT+GGG + chr6_2:4431574-4431593 None:intergenic 15.0%
!! ACAAATCAATAAATGAAAAA+AGG + chr6_2:4431668-4431687 None:intergenic 15.0%
!!! TCTCTTTTTTTACAAAAAAA+AGG - chr6_2:4431763-4431782 Msa0905970:intron 15.0%
!!! TAACAAAATAATTATTGGTT+TGG - chr6_2:4432354-4432373 Msa0905970:intron 15.0%
!! GTAAAAAATTGACAATTATT+TGG - chr6_2:4432740-4432759 Msa0905970:five_prime_UTR 15.0%
!!! ATCGATTTGATTATTAATTG+TGG - chr6_2:4431291-4431310 Msa0905970:intron 20.0%
!!! ATCGATTTGATTATTAATTG+TGG - chr6_2:4431291-4431310 Msa0905970:intron 20.0%
!! ATATTTCTCGTAATTATCAA+TGG - chr6_2:4431451-4431470 Msa0905970:intron 20.0%
!!! CCTTTTGTTTTTACAAATTT+TGG + chr6_2:4431575-4431594 None:intergenic 20.0%
!! CCAAAATTTGTAAAAACAAA+AGG - chr6_2:4431572-4431591 Msa0905970:intron 20.0%
!! AAATTTGTAAAAACAAAAGG+TGG - chr6_2:4431575-4431594 Msa0905970:intron 20.0%
!!! AATGTTGGCTAATAATTTTT+TGG + chr6_2:4431610-4431629 None:intergenic 20.0%
!! ATCGAAAAATAAACAAATGT+TGG + chr6_2:4431625-4431644 None:intergenic 20.0%
!!! TAATGAAGTTTTTTTTTACG+TGG - chr6_2:4431874-4431893 Msa0905970:intron 20.0%
!!! AATGAAGTTTTTTTTTACGT+GGG - chr6_2:4431875-4431894 Msa0905970:intron 20.0%
!! AATACAAGAGAAAAAGTTAA+AGG - chr6_2:4431942-4431961 Msa0905970:intron 20.0%
!!! GATGTTTGTTGTTAATTAAT+TGG + chr6_2:4432098-4432117 None:intergenic 20.0%
!! AAGTTATGTCTACTATAATA+TGG + chr6_2:4432225-4432244 None:intergenic 20.0%
!!! AATACATGTTACTTTTTCTT+AGG - chr6_2:4432787-4432806 Msa0905970:five_prime_UTR 20.0%
!!! ATCGATTTGATTATTAATTG+TGG - chr6_2:4431291-4431310 Msa0905970:intron 20.0%
!!! ATCGATTTGATTATTAATTG+TGG - chr6_2:4431291-4431310 Msa0905970:intron 20.0%
!! ATATTTCTCGTAATTATCAA+TGG - chr6_2:4431451-4431470 Msa0905970:intron 20.0%
!!! CCTTTTGTTTTTACAAATTT+TGG + chr6_2:4431575-4431594 None:intergenic 20.0%
!! CCAAAATTTGTAAAAACAAA+AGG - chr6_2:4431572-4431591 Msa0905970:intron 20.0%
!! AAATTTGTAAAAACAAAAGG+TGG - chr6_2:4431575-4431594 Msa0905970:intron 20.0%
!!! AATGTTGGCTAATAATTTTT+TGG + chr6_2:4431610-4431629 None:intergenic 20.0%
!! ATCGAAAAATAAACAAATGT+TGG + chr6_2:4431625-4431644 None:intergenic 20.0%
!!! TAATGAAGTTTTTTTTTACG+TGG - chr6_2:4431874-4431893 Msa0905970:intron 20.0%
!!! AATGAAGTTTTTTTTTACGT+GGG - chr6_2:4431875-4431894 Msa0905970:intron 20.0%
!! AATACAAGAGAAAAAGTTAA+AGG - chr6_2:4431942-4431961 Msa0905970:intron 20.0%
!!! GATGTTTGTTGTTAATTAAT+TGG + chr6_2:4432098-4432117 None:intergenic 20.0%
!! AAGTTATGTCTACTATAATA+TGG + chr6_2:4432225-4432244 None:intergenic 20.0%
!!! AATACATGTTACTTTTTCTT+AGG - chr6_2:4432787-4432806 Msa0905970:five_prime_UTR 20.0%
! ATATACACTTGTTCTATTGA+AGG - chr6_2:4430840-4430859 Msa0905970:three_prime_UTR 25.0%
!!! AGTGAGAATTTTTAAAGTGA+TGG - chr6_2:4430905-4430924 Msa0905970:exon 25.0%
!!! AAATTCAAGAGTTGTTTTGA+TGG - chr6_2:4430929-4430948 Msa0905970:CDS 25.0%
! TTGTTAAGATAAAGATCAGA+AGG - chr6_2:4431204-4431223 Msa0905970:CDS 25.0%
!!! GATTCATTGCAAACTTTTTT+TGG + chr6_2:4432065-4432084 None:intergenic 25.0%
!! TTTCGATTTTGTCTCTAATT+GGG + chr6_2:4432121-4432140 None:intergenic 25.0%
!! ATTTCGATTTTGTCTCTAAT+TGG + chr6_2:4432122-4432141 None:intergenic 25.0%
!! ACTTTTGTGAAATTCCATAT+TGG + chr6_2:4432145-4432164 None:intergenic 25.0%
!!! TGTTACTTTTTCTTAGGATT+TGG - chr6_2:4432793-4432812 Msa0905970:five_prime_UTR 25.0%
! ATATACACTTGTTCTATTGA+AGG - chr6_2:4430840-4430859 Msa0905970:three_prime_UTR 25.0%
!!! AGTGAGAATTTTTAAAGTGA+TGG - chr6_2:4430905-4430924 Msa0905970:exon 25.0%
!!! AAATTCAAGAGTTGTTTTGA+TGG - chr6_2:4430929-4430948 Msa0905970:CDS 25.0%
! TTGTTAAGATAAAGATCAGA+AGG - chr6_2:4431204-4431223 Msa0905970:CDS 25.0%
!!! GATTCATTGCAAACTTTTTT+TGG + chr6_2:4432065-4432084 None:intergenic 25.0%
!! TTTCGATTTTGTCTCTAATT+GGG + chr6_2:4432121-4432140 None:intergenic 25.0%
!! ATTTCGATTTTGTCTCTAAT+TGG + chr6_2:4432122-4432141 None:intergenic 25.0%
!! ACTTTTGTGAAATTCCATAT+TGG + chr6_2:4432145-4432164 None:intergenic 25.0%
!!! TGTTACTTTTTCTTAGGATT+TGG - chr6_2:4432793-4432812 Msa0905970:five_prime_UTR 25.0%
TATGTGTAAAGTGTGAAGTA+GGG + chr6_2:4430817-4430836 None:intergenic 30.0%
ATATGTGTAAAGTGTGAAGT+AGG + chr6_2:4430818-4430837 None:intergenic 30.0%
AGCATAGAAAGAATTGTGTT+AGG - chr6_2:4430865-4430884 Msa0905970:three_prime_UTR 30.0%
CAAATCGATAAACACAAAGA+CGG + chr6_2:4431280-4431299 None:intergenic 30.0%
CTCAATTTCTGCTTAGAATT+TGG - chr6_2:4431819-4431838 Msa0905970:intron 30.0%
!!! AAGTTTTTTTTTACGTGGGT+TGG - chr6_2:4431879-4431898 Msa0905970:intron 30.0%
ATTACCCTTGTGACTATAAT+GGG + chr6_2:4431925-4431944 None:intergenic 30.0%
TATTACCCTTGTGACTATAA+TGG + chr6_2:4431926-4431945 None:intergenic 30.0%
!!! CAAATCGTAGTTTTGAATAG+AGG + chr6_2:4432020-4432039 None:intergenic 30.0%
! TTCGATTTTGTCTCTAATTG+GGG + chr6_2:4432120-4432139 None:intergenic 30.0%
GACAAAATCGAAATCCAATA+TGG - chr6_2:4432128-4432147 Msa0905970:intron 30.0%
TCTTATTGCAACACTTGAAA+AGG - chr6_2:4432307-4432326 Msa0905970:intron 30.0%
TCTTGTCAAGTTTCTTATGA+AGG + chr6_2:4432462-4432481 None:intergenic 30.0%
TTCATAAGAAACTTGACAAG+AGG - chr6_2:4432461-4432480 Msa0905970:intron 30.0%
AAGACATACTACTTTATCAC+CGG - chr6_2:4432515-4432534 Msa0905970:intron 30.0%
TTCATTGTATCTGAAAGAGA+TGG + chr6_2:4432611-4432630 None:intergenic 30.0%
TCAGATACAATGAAATCTGA+TGG - chr6_2:4432617-4432636 Msa0905970:CDS 30.0%
CATCAATGTATACATGCTTT+GGG - chr6_2:4432654-4432673 Msa0905970:CDS 30.0%
TATGTGTAAAGTGTGAAGTA+GGG + chr6_2:4430817-4430836 None:intergenic 30.0%
ATATGTGTAAAGTGTGAAGT+AGG + chr6_2:4430818-4430837 None:intergenic 30.0%
AGCATAGAAAGAATTGTGTT+AGG - chr6_2:4430865-4430884 Msa0905970:three_prime_UTR 30.0%
CAAATCGATAAACACAAAGA+CGG + chr6_2:4431280-4431299 None:intergenic 30.0%
CTCAATTTCTGCTTAGAATT+TGG - chr6_2:4431819-4431838 Msa0905970:intron 30.0%
!!! AAGTTTTTTTTTACGTGGGT+TGG - chr6_2:4431879-4431898 Msa0905970:intron 30.0%
ATTACCCTTGTGACTATAAT+GGG + chr6_2:4431925-4431944 None:intergenic 30.0%
TATTACCCTTGTGACTATAA+TGG + chr6_2:4431926-4431945 None:intergenic 30.0%
!!! CAAATCGTAGTTTTGAATAG+AGG + chr6_2:4432020-4432039 None:intergenic 30.0%
! TTCGATTTTGTCTCTAATTG+GGG + chr6_2:4432120-4432139 None:intergenic 30.0%
GACAAAATCGAAATCCAATA+TGG - chr6_2:4432128-4432147 Msa0905970:intron 30.0%
TCTTATTGCAACACTTGAAA+AGG - chr6_2:4432307-4432326 Msa0905970:intron 30.0%
TCTTGTCAAGTTTCTTATGA+AGG + chr6_2:4432462-4432481 None:intergenic 30.0%
TTCATAAGAAACTTGACAAG+AGG - chr6_2:4432461-4432480 Msa0905970:intron 30.0%
AAGACATACTACTTTATCAC+CGG - chr6_2:4432515-4432534 Msa0905970:intron 30.0%
TTCATTGTATCTGAAAGAGA+TGG + chr6_2:4432611-4432630 None:intergenic 30.0%
TCAGATACAATGAAATCTGA+TGG - chr6_2:4432617-4432636 Msa0905970:CDS 30.0%
CATCAATGTATACATGCTTT+GGG - chr6_2:4432654-4432673 Msa0905970:CDS 30.0%
!! TTTTGGGAATGTTGTCATTG+TGG - chr6_2:4430961-4430980 Msa0905970:CDS 35.0%
AGAATGTCAACTACACTACA+TGG - chr6_2:4431045-4431064 Msa0905970:CDS 35.0%
CATACTTCTGCTTCATATCA+TGG + chr6_2:4431248-4431267 None:intergenic 35.0%
! TGCAACACTTGAAAAGGTTT+TGG - chr6_2:4432313-4432332 Msa0905970:intron 35.0%
CAAAAGATACTACAACGTCT+TGG - chr6_2:4432397-4432416 Msa0905970:intron 35.0%
ATAAGAAACTTGACAAGAGG+TGG - chr6_2:4432464-4432483 Msa0905970:intron 35.0%
GATACAATGAAATCTGATGG+TGG - chr6_2:4432620-4432639 Msa0905970:CDS 35.0%
!!! ATCTGATGGTGGTTCTATTT+TGG - chr6_2:4432631-4432650 Msa0905970:CDS 35.0%
GCATCAATGTATACATGCTT+TGG - chr6_2:4432653-4432672 Msa0905970:CDS 35.0%
TGGGATTATTGTTGCTAATG+TGG - chr6_2:4432673-4432692 Msa0905970:CDS 35.0%
!!! TATGTGTGCTTGGTTTTAGT+GGG - chr6_2:4432698-4432717 Msa0905970:CDS 35.0%
! TGGGGATTTTGTAAGAGATT+AGG - chr6_2:4432717-4432736 Msa0905970:exon 35.0%
! GGGGATTTTGTAAGAGATTA+GGG - chr6_2:4432718-4432737 Msa0905970:exon 35.0%
!! TTTTGGGAATGTTGTCATTG+TGG - chr6_2:4430961-4430980 Msa0905970:CDS 35.0%
AGAATGTCAACTACACTACA+TGG - chr6_2:4431045-4431064 Msa0905970:CDS 35.0%
CATACTTCTGCTTCATATCA+TGG + chr6_2:4431248-4431267 None:intergenic 35.0%
! TGCAACACTTGAAAAGGTTT+TGG - chr6_2:4432313-4432332 Msa0905970:intron 35.0%
CAAAAGATACTACAACGTCT+TGG - chr6_2:4432397-4432416 Msa0905970:intron 35.0%
ATAAGAAACTTGACAAGAGG+TGG - chr6_2:4432464-4432483 Msa0905970:intron 35.0%
GATACAATGAAATCTGATGG+TGG - chr6_2:4432620-4432639 Msa0905970:CDS 35.0%
!!! ATCTGATGGTGGTTCTATTT+TGG - chr6_2:4432631-4432650 Msa0905970:CDS 35.0%
GCATCAATGTATACATGCTT+TGG - chr6_2:4432653-4432672 Msa0905970:CDS 35.0%
TGGGATTATTGTTGCTAATG+TGG - chr6_2:4432673-4432692 Msa0905970:CDS 35.0%
!!! TATGTGTGCTTGGTTTTAGT+GGG - chr6_2:4432698-4432717 Msa0905970:CDS 35.0%
! TGGGGATTTTGTAAGAGATT+AGG - chr6_2:4432717-4432736 Msa0905970:exon 35.0%
! GGGGATTTTGTAAGAGATTA+GGG - chr6_2:4432718-4432737 Msa0905970:exon 35.0%
!!! AGTTGTTTTGATGGTGAGTG+TGG - chr6_2:4430938-4430957 Msa0905970:CDS 40.0%
!!! TTTGATGGTGAGTGTGGTTT+TGG - chr6_2:4430944-4430963 Msa0905970:CDS 40.0%
!!! TTGATGGTGAGTGTGGTTTT+GGG - chr6_2:4430945-4430964 Msa0905970:CDS 40.0%
AATGTTGTCATTGTGGCCAA+TGG - chr6_2:4430968-4430987 Msa0905970:CDS 40.0%
GACATTCTCTTTCCAACCTT+TGG + chr6_2:4431033-4431052 None:intergenic 40.0%
! TCTCAAGAGCATGTCTATGT+TGG - chr6_2:4431071-4431090 Msa0905970:CDS 40.0%
!! AGCATGTCTATGTTGGTGAT+TGG - chr6_2:4431078-4431097 Msa0905970:CDS 40.0%
!!! TTTTACGTGGGTTGGAGTAT+TGG - chr6_2:4431887-4431906 Msa0905970:intron 40.0%
!! ACTATAATGGGTGGCATACT+TGG + chr6_2:4431913-4431932 None:intergenic 40.0%
ACATACTACTTTATCACCGG+TGG - chr6_2:4432518-4432537 Msa0905970:intron 40.0%
GTATCTGAAAGAGATGGAGA+TGG + chr6_2:4432605-4432624 None:intergenic 40.0%
TAATGTGGTGTATGTGTGCT+TGG - chr6_2:4432688-4432707 Msa0905970:CDS 40.0%
!!! GTATGTGTGCTTGGTTTTAG+TGG - chr6_2:4432697-4432716 Msa0905970:CDS 40.0%
!!! ATGTGTGCTTGGTTTTAGTG+GGG - chr6_2:4432699-4432718 Msa0905970:CDS 40.0%
!!! AGTTGTTTTGATGGTGAGTG+TGG - chr6_2:4430938-4430957 Msa0905970:CDS 40.0%
!!! TTTGATGGTGAGTGTGGTTT+TGG - chr6_2:4430944-4430963 Msa0905970:CDS 40.0%
!!! TTGATGGTGAGTGTGGTTTT+GGG - chr6_2:4430945-4430964 Msa0905970:CDS 40.0%
AATGTTGTCATTGTGGCCAA+TGG - chr6_2:4430968-4430987 Msa0905970:CDS 40.0%
GACATTCTCTTTCCAACCTT+TGG + chr6_2:4431033-4431052 None:intergenic 40.0%
! TCTCAAGAGCATGTCTATGT+TGG - chr6_2:4431071-4431090 Msa0905970:CDS 40.0%
!! AGCATGTCTATGTTGGTGAT+TGG - chr6_2:4431078-4431097 Msa0905970:CDS 40.0%
!!! TTTTACGTGGGTTGGAGTAT+TGG - chr6_2:4431887-4431906 Msa0905970:intron 40.0%
!! ACTATAATGGGTGGCATACT+TGG + chr6_2:4431913-4431932 None:intergenic 40.0%
ACATACTACTTTATCACCGG+TGG - chr6_2:4432518-4432537 Msa0905970:intron 40.0%
GTATCTGAAAGAGATGGAGA+TGG + chr6_2:4432605-4432624 None:intergenic 40.0%
TAATGTGGTGTATGTGTGCT+TGG - chr6_2:4432688-4432707 Msa0905970:CDS 40.0%
!!! GTATGTGTGCTTGGTTTTAG+TGG - chr6_2:4432697-4432716 Msa0905970:CDS 40.0%
!!! ATGTGTGCTTGGTTTTAGTG+GGG - chr6_2:4432699-4432718 Msa0905970:CDS 40.0%
AGGTCCAAAGCTTCATAAGG+TGG - chr6_2:4431001-4431020 Msa0905970:CDS 45.0%
GGTCCAAAGCTTCATAAGGT+GGG - chr6_2:4431002-4431021 Msa0905970:CDS 45.0%
TGCCACCCATTATAGTCACA+AGG - chr6_2:4431917-4431936 Msa0905970:intron 45.0%
GCCACCCATTATAGTCACAA+GGG - chr6_2:4431918-4431937 Msa0905970:intron 45.0%
! ACCCTTGTGACTATAATGGG+TGG + chr6_2:4431922-4431941 None:intergenic 45.0%
GTCTTGGAAGTGAACAAGAC+AGG - chr6_2:4432413-4432432 Msa0905970:intron 45.0%
TCGCGATGTGGTTCAATTGA+CGG - chr6_2:4432487-4432506 Msa0905970:intron 45.0%
TACTACTTTATCACCGGTGG+AGG - chr6_2:4432521-4432540 Msa0905970:intron 45.0%
ATGAAAGCAGTAACCTCCAC+CGG + chr6_2:4432537-4432556 None:intergenic 45.0%
TCATGGAATGAAAGTTGCCG+TGG - chr6_2:4432553-4432572 Msa0905970:CDS 45.0%
! TTGGTGTTCTTGAACGTCCA+CGG + chr6_2:4432573-4432592 None:intergenic 45.0%
AGGTCCAAAGCTTCATAAGG+TGG - chr6_2:4431001-4431020 Msa0905970:CDS 45.0%
GGTCCAAAGCTTCATAAGGT+GGG - chr6_2:4431002-4431021 Msa0905970:CDS 45.0%
TGCCACCCATTATAGTCACA+AGG - chr6_2:4431917-4431936 Msa0905970:intron 45.0%
GCCACCCATTATAGTCACAA+GGG - chr6_2:4431918-4431937 Msa0905970:intron 45.0%
! ACCCTTGTGACTATAATGGG+TGG + chr6_2:4431922-4431941 None:intergenic 45.0%
GTCTTGGAAGTGAACAAGAC+AGG - chr6_2:4432413-4432432 Msa0905970:intron 45.0%
TCGCGATGTGGTTCAATTGA+CGG - chr6_2:4432487-4432506 Msa0905970:intron 45.0%
TACTACTTTATCACCGGTGG+AGG - chr6_2:4432521-4432540 Msa0905970:intron 45.0%
ATGAAAGCAGTAACCTCCAC+CGG + chr6_2:4432537-4432556 None:intergenic 45.0%
TCATGGAATGAAAGTTGCCG+TGG - chr6_2:4432553-4432572 Msa0905970:CDS 45.0%
! TTGGTGTTCTTGAACGTCCA+CGG + chr6_2:4432573-4432592 None:intergenic 45.0%
GTTGTCATTGTGGCCAATGG+TGG - chr6_2:4430971-4430990 Msa0905970:CDS 50.0%
ATTGTGGCCAATGGTGGTCA+TGG - chr6_2:4430977-4430996 Msa0905970:CDS 50.0%
TTGTGGCCAATGGTGGTCAT+GGG - chr6_2:4430978-4430997 Msa0905970:CDS 50.0%
GGGAGGTCCAAAGCTTCATA+AGG - chr6_2:4430998-4431017 Msa0905970:CDS 50.0%
CCTCCCACCTTATGAAGCTT+TGG + chr6_2:4431008-4431027 None:intergenic 50.0%
CCAAAGCTTCATAAGGTGGG+AGG - chr6_2:4431005-4431024 Msa0905970:CDS 50.0%
CATAAGGTGGGAGGATCCAA+AGG - chr6_2:4431014-4431033 Msa0905970:CDS 50.0%
CGATGTGGTTCAATTGACGG+AGG - chr6_2:4432490-4432509 Msa0905970:intron 50.0%
GGTGGAGGTTACTGCTTTCA+TGG - chr6_2:4432536-4432555 Msa0905970:intron 50.0%
ATGGAGATGGTGCAGGTGAT+TGG + chr6_2:4432592-4432611 None:intergenic 50.0%
GAAAGAGATGGAGATGGTGC+AGG + chr6_2:4432599-4432618 None:intergenic 50.0%
GTTGTCATTGTGGCCAATGG+TGG - chr6_2:4430971-4430990 Msa0905970:CDS 50.0%
ATTGTGGCCAATGGTGGTCA+TGG - chr6_2:4430977-4430996 Msa0905970:CDS 50.0%
TTGTGGCCAATGGTGGTCAT+GGG - chr6_2:4430978-4430997 Msa0905970:CDS 50.0%
GGGAGGTCCAAAGCTTCATA+AGG - chr6_2:4430998-4431017 Msa0905970:CDS 50.0%
CCTCCCACCTTATGAAGCTT+TGG + chr6_2:4431008-4431027 None:intergenic 50.0%
CCAAAGCTTCATAAGGTGGG+AGG - chr6_2:4431005-4431024 Msa0905970:CDS 50.0%
CATAAGGTGGGAGGATCCAA+AGG - chr6_2:4431014-4431033 Msa0905970:CDS 50.0%
CGATGTGGTTCAATTGACGG+AGG - chr6_2:4432490-4432509 Msa0905970:intron 50.0%
GGTGGAGGTTACTGCTTTCA+TGG - chr6_2:4432536-4432555 Msa0905970:intron 50.0%
ATGGAGATGGTGCAGGTGAT+TGG + chr6_2:4432592-4432611 None:intergenic 50.0%
GAAAGAGATGGAGATGGTGC+AGG + chr6_2:4432599-4432618 None:intergenic 50.0%
AGGTGGGAGGATCCAAAGGT+TGG - chr6_2:4431018-4431037 Msa0905970:CDS 55.0%
AGGTGGGAGGATCCAAAGGT+TGG - chr6_2:4431018-4431037 Msa0905970:CDS 55.0%
TGGCCAATGGTGGTCATGGG+AGG - chr6_2:4430981-4431000 Msa0905970:CDS 60.0%
GGACCTCCCATGACCACCAT+TGG + chr6_2:4430987-4431006 None:intergenic 60.0%
GACAAGAGGTGGTCGCGATG+TGG - chr6_2:4432475-4432494 Msa0905970:intron 60.0%
TGGCCAATGGTGGTCATGGG+AGG - chr6_2:4430981-4431000 Msa0905970:CDS 60.0%
GGACCTCCCATGACCACCAT+TGG + chr6_2:4430987-4431006 None:intergenic 60.0%
GACAAGAGGTGGTCGCGATG+TGG - chr6_2:4432475-4432494 Msa0905970:intron 60.0%
Chromosome Type Strat End Strand Name
chr6_2 gene 4430814 4432828 4430814 ID=Msa0905970;Name=Msa0905970
chr6_2 mRNA 4430814 4432828 4430814 ID=Msa0905970-mRNA-1;Parent=Msa0905970;Name=Msa0905970-mRNA-1;_AED=0.00;_eAED=0.00;_QI=110|1|1|1|1|1|2|98|175
chr6_2 exon 4430814 4431258 4430814 ID=Msa0905970-mRNA-1:exon:1281;Parent=Msa0905970-mRNA-1
chr6_2 exon 4432538 4432828 4432538 ID=Msa0905970-mRNA-1:exon:1280;Parent=Msa0905970-mRNA-1
chr6_2 five_prime_UTR 4432719 4432828 4432719 ID=Msa0905970-mRNA-1:five_prime_utr;Parent=Msa0905970-mRNA-1
chr6_2 CDS 4432538 4432718 4432538 ID=Msa0905970-mRNA-1:cds;Parent=Msa0905970-mRNA-1
chr6_2 CDS 4430912 4431258 4430912 ID=Msa0905970-mRNA-1:cds;Parent=Msa0905970-mRNA-1
chr6_2 three_prime_UTR 4430814 4430911 4430814 ID=Msa0905970-mRNA-1:three_prime_utr;Parent=Msa0905970-mRNA-1
Gene Sequence

>Msa0905970

ATGGAAAATTCAAGAGTTGTTTTGATGGTGAGTGTGGTTTTGGGAATGTTGTCATTGTGGCCAATGGTGGTCATGGGAGGTCCAAAGCTTCATAAGGTGGGAGGATCCAAAGGTTGGAAAGAGAATGTCAACTACACTACATGGTCTTCTCAAGAGCATGTCTATGTTGGTGATTGGCTAAAATTTGTCTTTGACAAAAGATACTACAACGTCTTGGAAGTGAACAAGACAGGCTATGAATATTGCATTGACATGACCTTCATAAGAAACTTGACAAGAGGTGGTCGCGATGTGGTTCAATTGACGGAGGCAAAGACATACTACTTTATCACCGGTGGAGGTTACTGCTTTCATGGAATGAAAGTTGCCGTGGACGTTCAAGAACACCAATCACCTGCACCATCTCCATCTCTTTCAGATACAATGAAATCTGATGGTGGTTCTATTTTGGCATCAATGTATACATGCTTTGGGATTATTGTTGCTAATGTGGTGTATGTGTGCTTGGTTTTAGTGGGGATTTTGTAA
Protein sequence

>Msa0905970

MENSRVVLMVSVVLGMLSLWPMVVMGGPKLHKVGGSKGWKENVNYTTWSSQEHVYVGDWLKFVFDKRYYNVLEVNKTGYEYCIDMTFIRNLTRGGRDVVQLTEAKTYYFITGGGYCFHGMKVAVDVQEHQSPAPSPSLSDTMKSDGGSILASMYTCFGIIVANVVYVCLVLVGIL*