Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0931050 | AWW91873.1 | 100.000 | 220 | 0 | 0 | 1 | 220 | 1 | 220 | 1.59e-147 | 448 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0931050 | sp|Q9BBS9|RPOB_LOTJA | 95.814 | 215 | 9 | 0 | 6 | 220 | 1 | 215 | 2.10e-141 | 424 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0139790 | Msa0931050 | 0.808275 | 3.355195e-50 | -8.615850e-47 |
| Msa0268190 | Msa0931050 | 0.807291 | 5.431275e-50 | -8.615850e-47 |
| Msa0268350 | Msa0931050 | 0.809869 | 1.528738e-50 | -8.615850e-47 |
| Msa0268430 | Msa0931050 | 0.816337 | 5.824937e-52 | -8.615850e-47 |
| Msa0268570 | Msa0931050 | 0.809684 | 1.675271e-50 | -8.615850e-47 |
| Msa0268660 | Msa0931050 | 0.821447 | 4.018262e-53 | -8.615850e-47 |
| Msa0268750 | Msa0931050 | 0.802454 | 5.566592e-49 | -8.615850e-47 |
| Msa0268860 | Msa0931050 | 0.807205 | 5.663267e-50 | -8.615850e-47 |
| Msa0269070 | Msa0931050 | 0.816078 | 6.656810e-52 | -8.615850e-47 |
| Msa0269090 | Msa0931050 | 0.802739 | 4.862945e-49 | -8.615850e-47 |
| Msa0484000 | Msa0931050 | 0.846685 | 1.859467e-59 | -8.615850e-47 |
| Msa0582500 | Msa0931050 | 0.856146 | 3.917886e-62 | -8.615850e-47 |
| Msa0582540 | Msa0931050 | 0.804620 | 1.978951e-49 | -8.615850e-47 |
| Msa0582650 | Msa0931050 | 0.801993 | 6.926471e-49 | -8.615850e-47 |
| Msa0582670 | Msa0931050 | 0.848073 | 7.730955e-60 | -8.615850e-47 |
| Msa0582680 | Msa0931050 | 0.859249 | 4.712220e-63 | -8.615850e-47 |
| Msa0582730 | Msa0931050 | 0.821404 | 4.111138e-53 | -8.615850e-47 |
| Msa0582770 | Msa0931050 | 0.805549 | 1.265073e-49 | -8.615850e-47 |
| Msa0582790 | Msa0931050 | 0.808324 | 3.275037e-50 | -8.615850e-47 |
| Msa0582800 | Msa0931050 | 0.880407 | 5.631652e-70 | -8.615850e-47 |
| Msa0582840 | Msa0931050 | 0.809145 | 2.186712e-50 | -8.615850e-47 |
| Msa0582890 | Msa0931050 | 0.801249 | 9.844998e-49 | -8.615850e-47 |
| Msa0931040 | Msa0931050 | 0.832182 | 1.094073e-55 | -8.615850e-47 |
| Msa0931050 | Msa0931110 | 0.814208 | 1.731794e-51 | -8.615850e-47 |
| Msa0931050 | Msa0931120 | 0.859652 | 3.566798e-63 | -8.615850e-47 |
| Msa0931050 | Msa0931250 | 0.841708 | 4.033356e-58 | -8.615850e-47 |
| Msa0931050 | Msa1086240 | 0.844614 | 6.779748e-59 | -8.615850e-47 |
| Msa0931050 | Msa1086490 | 0.815954 | 7.093499e-52 | -8.615850e-47 |
| Msa0931050 | Msa1086670 | 0.826054 | 3.348043e-54 | -8.615850e-47 |
| Msa0931050 | Msa1143100 | 0.864390 | 1.258602e-64 | -8.615850e-47 |
| Msa0931050 | Msa1143190 | 0.803559 | 3.290264e-49 | -8.615850e-47 |
| Msa0931050 | Msa1305100 | 0.831024 | 2.109685e-55 | -8.615850e-47 |
| Msa0931050 | Msa1305160 | 0.832337 | 1.001249e-55 | -8.615850e-47 |
| Msa0931050 | Msa1342050 | 0.809841 | 1.550310e-50 | -8.615850e-47 |
| Msa0931050 | Msa1354940 | 0.847452 | 1.146528e-59 | -8.615850e-47 |
| Msa0931050 | Msa1355120 | 0.866113 | 3.614204e-65 | -8.615850e-47 |
| Msa0931050 | Msa1380630 | 0.822160 | 2.748421e-53 | -8.615850e-47 |
| Msa0931050 | Msa1380700 | 0.834836 | 2.380581e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0931050 | MtrunA17_Chr4g0016641 | 99.091 | 220 | 2 | 0 | 1 | 220 | 1 | 220 | 7.08e-150 | 443 |
| Msa0931050 | MtrunA17_CPg0493041 | 99.091 | 220 | 2 | 0 | 1 | 220 | 1 | 220 | 7.08e-150 | 443 |
| Msa0931050 | MtrunA17_Chr4g0015521 | 98.165 | 109 | 2 | 0 | 112 | 220 | 1 | 109 | 4.57e-73 | 217 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0931050 | ATCG00190.1 | 86.977 | 215 | 28 | 0 | 6 | 220 | 1 | 215 | 2.10e-125 | 380 |
Find 44 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATATCCGCGGGATTAATT+TGG | 0.209384 | 6_2:+80305284 | Msa0931050:CDS |
| CTGGGAACTTCTATAGTAAA+TGG | 0.231551 | 6_2:+80305370 | Msa0931050:CDS |
| TTGATAGAAAAGCAAGGATA+TGG | 0.252693 | 6_2:+80305527 | Msa0931050:CDS |
| AAATTGTATTTGATTTAATC+CGG | 0.349804 | 6_2:-80305080 | None:intergenic |
| TGGGGAAATTGAAAATGCTT+GGG | 0.386996 | 6_2:+80305029 | Msa0931050:CDS |
| AATTGAAAATGCTTGGGGTT+GGG | 0.394420 | 6_2:+80305035 | Msa0931050:CDS |
| CATTCCTCTAATGAATTCCC+TGG | 0.423604 | 6_2:+80305351 | Msa0931050:CDS |
| ATGGGGAAATTGAAAATGCT+TGG | 0.425882 | 6_2:+80305028 | Msa0931050:CDS |
| TATGTCTTTCCTCAATGAAA+AGG | 0.429238 | 6_2:+80305654 | Msa0931050:CDS |
| ATGCTTGGGGTTGGGAATGA+AGG | 0.438598 | 6_2:+80305043 | Msa0931050:CDS |
| TGGCACAATAATATCCGATT+GGG | 0.439186 | 6_2:+80305483 | Msa0931050:CDS |
| AATTGAATTTCAGTTATTTG+TGG | 0.443334 | 6_2:+80305183 | Msa0931050:CDS |
| GTTCCCAGGGAATTCATTAG+AGG | 0.450312 | 6_2:-80305355 | None:intergenic |
| AACGGAATTTCAGTCTATAC+TGG | 0.455189 | 6_2:+80305463 | Msa0931050:CDS |
| AAATTGAAAATGCTTGGGGT+TGG | 0.457271 | 6_2:+80305034 | Msa0931050:CDS |
| TTAAATCAAATACAATTTGA+AGG | 0.460401 | 6_2:+80305085 | Msa0931050:CDS |
| ATTTGTGGAAACATATCAAT+TGG | 0.470517 | 6_2:+80305198 | Msa0931050:CDS |
| CATTTACTATAGAAGTTCCC+AGG | 0.473812 | 6_2:-80305369 | None:intergenic |
| TCTAGTTCTATCATCAGCTA+TGG | 0.479428 | 6_2:+80305582 | Msa0931050:CDS |
| TATAGACTGAAATTCCGTTA+TGG | 0.489213 | 6_2:-80305459 | None:intergenic |
| CGTTCAGAGTTGGACCATAA+CGG | 0.495197 | 6_2:+80305445 | Msa0931050:CDS |
| TATCTATTATCGTTCAGAGT+TGG | 0.510246 | 6_2:+80305435 | Msa0931050:CDS |
| CTGGCACAATAATATCCGAT+TGG | 0.523488 | 6_2:+80305482 | Msa0931050:CDS |
| TGATAGAAAAGCAAGGATAT+GGG | 0.526528 | 6_2:+80305528 | Msa0931050:CDS |
| CTAGTTCTATCATCAGCTAT+GGG | 0.528023 | 6_2:+80305583 | Msa0931050:CDS |
| TAAATCAAATACAATTTGAA+GGG | 0.532005 | 6_2:+80305086 | Msa0931050:CDS |
| GGATATGGGCTCGTGTAAGT+AGG | 0.539731 | 6_2:+80305542 | Msa0931050:CDS |
| TAGAAATTGATAGAAAAGCA+AGG | 0.548786 | 6_2:+80305521 | Msa0931050:CDS |
| AATTGTATTTGATTTAATCC+GGG | 0.549785 | 6_2:-80305079 | None:intergenic |
| TCTGAATTATATATATCCGC+GGG | 0.562320 | 6_2:+80305274 | Msa0931050:CDS |
| AATCAAATATTGCAAAGCCC+TGG | 0.569397 | 6_2:+80305412 | Msa0931050:CDS |
| CACAATAATATCCGATTGGG+GGG | 0.571960 | 6_2:+80305486 | Msa0931050:CDS |
| CTCTGAATTATATATATCCG+CGG | 0.577667 | 6_2:+80305273 | Msa0931050:CDS |
| GTCTTTCCTCAATGAAAAGG+AGG | 0.586724 | 6_2:+80305657 | Msa0931050:CDS |
| CGCGGATATATATAATTCAG+AGG | 0.586801 | 6_2:-80305272 | None:intergenic |
| ATTTACTATAGAAGTTCCCA+GGG | 0.587463 | 6_2:-80305368 | None:intergenic |
| TCTGAACGATAATAGATACC+AGG | 0.589979 | 6_2:-80305430 | None:intergenic |
| ATTCCTCTAATGAATTCCCT+GGG | 0.606971 | 6_2:+80305352 | Msa0931050:CDS |
| GGCACAATAATATCCGATTG+GGG | 0.627268 | 6_2:+80305484 | Msa0931050:CDS |
| GAAGGAATGTCTACACTACC+CGG | 0.627870 | 6_2:+80305061 | Msa0931050:CDS |
| ACAATAATATCCGATTGGGG+GGG | 0.637953 | 6_2:+80305487 | Msa0931050:CDS |
| CTGAACGATAATAGATACCA+GGG | 0.641198 | 6_2:-80305429 | None:intergenic |
| GGGGAAATTGAAAATGCTTG+GGG | 0.643696 | 6_2:+80305030 | Msa0931050:CDS |
| GCACAATAATATCCGATTGG+GGG | 0.683666 | 6_2:+80305485 | Msa0931050:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTGTATTTGATTTAATC+CGG | - | chr6_2:80305083-80305102 | None:intergenic | 15.0% |
| !! | TTAAATCAAATACAATTTGA+AGG | + | chr6_2:80305085-80305104 | Msa0931050:CDS | 15.0% |
| !! | TAAATCAAATACAATTTGAA+GGG | + | chr6_2:80305086-80305105 | Msa0931050:CDS | 15.0% |
| !!! | CAATAAAAATAATTTGTTCT+TGG | - | chr6_2:80305327-80305346 | None:intergenic | 15.0% |
| !!! | CAAGAACAAATTATTTTTAT+TGG | + | chr6_2:80305325-80305344 | Msa0931050:CDS | 15.0% |
| !!! | AATTGTATTTGATTTAATCC+GGG | - | chr6_2:80305082-80305101 | None:intergenic | 20.0% |
| !! | AATTGAATTTCAGTTATTTG+TGG | + | chr6_2:80305183-80305202 | Msa0931050:CDS | 20.0% |
| !!! | ATTAATTTGGAAAAACAGTA+GGG | + | chr6_2:80305297-80305316 | Msa0931050:CDS | 20.0% |
| ! | GTTTCCAAAAATTGAAGATA+CGG | + | chr6_2:80305153-80305172 | Msa0931050:CDS | 25.0% |
| ! | ATTTGTGGAAACATATCAAT+TGG | + | chr6_2:80305198-80305217 | Msa0931050:CDS | 25.0% |
| !! | GATTAATTTGGAAAAACAGT+AGG | + | chr6_2:80305296-80305315 | Msa0931050:CDS | 25.0% |
| ! | TAGAAATTGATAGAAAAGCA+AGG | + | chr6_2:80305521-80305540 | Msa0931050:CDS | 25.0% |
| ! | AGGAAAGACATAAAAATTTC+GGG | - | chr6_2:80305646-80305665 | None:intergenic | 25.0% |
| ! | GAGGAAAGACATAAAAATTT+CGG | - | chr6_2:80305647-80305666 | None:intergenic | 25.0% |
| !! | GGTTTTGTAGATTCATTGAT+CGG | + | chr6_2:80305107-80305126 | Msa0931050:CDS | 30.0% |
| ! | GTTTTGTAGATTCATTGATC+GGG | + | chr6_2:80305108-80305127 | Msa0931050:CDS | 30.0% |
| ! | TTTTGTAGATTCATTGATCG+GGG | + | chr6_2:80305109-80305128 | Msa0931050:CDS | 30.0% |
| ! | TGATCCGTATCTTCAATTTT+TGG | - | chr6_2:80305160-80305179 | None:intergenic | 30.0% |
| ! | GCATCTTTTTCTTTTATCGA+GGG | - | chr6_2:80305229-80305248 | None:intergenic | 30.0% |
| ! | AGCATCTTTTTCTTTTATCG+AGG | - | chr6_2:80305230-80305249 | None:intergenic | 30.0% |
| CTCTGAATTATATATATCCG+CGG | + | chr6_2:80305273-80305292 | Msa0931050:CDS | 30.0% | |
| TCTGAATTATATATATCCGC+GGG | + | chr6_2:80305274-80305293 | Msa0931050:CDS | 30.0% | |
| ATTTACTATAGAAGTTCCCA+GGG | - | chr6_2:80305371-80305390 | None:intergenic | 30.0% | |
| TATCTATTATCGTTCAGAGT+TGG | + | chr6_2:80305435-80305454 | Msa0931050:CDS | 30.0% | |
| TATAGACTGAAATTCCGTTA+TGG | - | chr6_2:80305462-80305481 | None:intergenic | 30.0% | |
| TTGATAGAAAAGCAAGGATA+TGG | + | chr6_2:80305527-80305546 | Msa0931050:CDS | 30.0% | |
| TGATAGAAAAGCAAGGATAT+GGG | + | chr6_2:80305528-80305547 | Msa0931050:CDS | 30.0% | |
| GGAAAGACATAAAAATTTCG+GGG | - | chr6_2:80305645-80305664 | None:intergenic | 30.0% | |
| TATGTCTTTCCTCAATGAAA+AGG | + | chr6_2:80305654-80305673 | Msa0931050:CDS | 30.0% | |
| !!! | TTTTTTCCTCCTTTTCATTG+AGG | - | chr6_2:80305666-80305685 | None:intergenic | 30.0% |
| ATGGGGAAATTGAAAATGCT+TGG | + | chr6_2:80305028-80305047 | Msa0931050:CDS | 35.0% | |
| TGGGGAAATTGAAAATGCTT+GGG | + | chr6_2:80305029-80305048 | Msa0931050:CDS | 35.0% | |
| AAATTGAAAATGCTTGGGGT+TGG | + | chr6_2:80305034-80305053 | Msa0931050:CDS | 35.0% | |
| AATTGAAAATGCTTGGGGTT+GGG | + | chr6_2:80305035-80305054 | Msa0931050:CDS | 35.0% | |
| CGCGGATATATATAATTCAG+AGG | - | chr6_2:80305275-80305294 | None:intergenic | 35.0% | |
| ATATATCCGCGGGATTAATT+TGG | + | chr6_2:80305284-80305303 | Msa0931050:CDS | 35.0% | |
| ! | GTTTTTCCAAATTAATCCCG+CGG | - | chr6_2:80305293-80305312 | None:intergenic | 35.0% |
| ATTCCTCTAATGAATTCCCT+GGG | + | chr6_2:80305352-80305371 | Msa0931050:CDS | 35.0% | |
| CATTTACTATAGAAGTTCCC+AGG | - | chr6_2:80305372-80305391 | None:intergenic | 35.0% | |
| CTGGGAACTTCTATAGTAAA+TGG | + | chr6_2:80305370-80305389 | Msa0931050:CDS | 35.0% | |
| AATCAAATATTGCAAAGCCC+TGG | + | chr6_2:80305412-80305431 | Msa0931050:CDS | 35.0% | |
| CTGAACGATAATAGATACCA+GGG | - | chr6_2:80305432-80305451 | None:intergenic | 35.0% | |
| TCTGAACGATAATAGATACC+AGG | - | chr6_2:80305433-80305452 | None:intergenic | 35.0% | |
| AACGGAATTTCAGTCTATAC+TGG | + | chr6_2:80305463-80305482 | Msa0931050:CDS | 35.0% | |
| TGGCACAATAATATCCGATT+GGG | + | chr6_2:80305483-80305502 | Msa0931050:CDS | 35.0% | |
| !! | TCTAGTTCTATCATCAGCTA+TGG | + | chr6_2:80305582-80305601 | Msa0931050:CDS | 35.0% |
| !! | CTAGTTCTATCATCAGCTAT+GGG | + | chr6_2:80305583-80305602 | Msa0931050:CDS | 35.0% |
| GGGGAAATTGAAAATGCTTG+GGG | + | chr6_2:80305030-80305049 | Msa0931050:CDS | 40.0% | |
| CATTCCTCTAATGAATTCCC+TGG | + | chr6_2:80305351-80305370 | Msa0931050:CDS | 40.0% | |
| CTGGCACAATAATATCCGAT+TGG | + | chr6_2:80305482-80305501 | Msa0931050:CDS | 40.0% | |
| GGCACAATAATATCCGATTG+GGG | + | chr6_2:80305484-80305503 | Msa0931050:CDS | 40.0% | |
| GCACAATAATATCCGATTGG+GGG | + | chr6_2:80305485-80305504 | Msa0931050:CDS | 40.0% | |
| CACAATAATATCCGATTGGG+GGG | + | chr6_2:80305486-80305505 | Msa0931050:CDS | 40.0% | |
| ACAATAATATCCGATTGGGG+GGG | + | chr6_2:80305487-80305506 | Msa0931050:CDS | 40.0% | |
| GTCTTTCCTCAATGAAAAGG+AGG | + | chr6_2:80305657-80305676 | Msa0931050:CDS | 40.0% | |
| GAAGGAATGTCTACACTACC+CGG | + | chr6_2:80305061-80305080 | Msa0931050:CDS | 45.0% | |
| GTTCCCAGGGAATTCATTAG+AGG | - | chr6_2:80305358-80305377 | None:intergenic | 45.0% | |
| CGTTCAGAGTTGGACCATAA+CGG | + | chr6_2:80305445-80305464 | Msa0931050:CDS | 45.0% | |
| ATGCTTGGGGTTGGGAATGA+AGG | + | chr6_2:80305043-80305062 | Msa0931050:CDS | 50.0% | |
| CTCTAATCTTCCCCCCCAAT+CGG | - | chr6_2:80305500-80305519 | None:intergenic | 50.0% | |
| GGATATGGGCTCGTGTAAGT+AGG | + | chr6_2:80305542-80305561 | Msa0931050:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 80305028 | 80305690 | 80305028 | ID=Msa0931050;Name=Msa0931050 |
| chr6_2 | mRNA | 80305028 | 80305690 | 80305028 | ID=Msa0931050-mRNA-1;Parent=Msa0931050;Name=Msa0931050-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|220 |
| chr6_2 | exon | 80305028 | 80305690 | 80305028 | ID=Msa0931050-mRNA-1:exon:14057;Parent=Msa0931050-mRNA-1 |
| chr6_2 | CDS | 80305028 | 80305690 | 80305028 | ID=Msa0931050-mRNA-1:cds;Parent=Msa0931050-mRNA-1 |
| Gene Sequence |
| Protein sequence |
