Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0610430 | Msa0940380 | 0.807127 | 5.882062e-50 | -8.615850e-47 |
| Msa0617640 | Msa0940380 | 0.806033 | 1.001238e-49 | -8.615850e-47 |
| Msa0663390 | Msa0940380 | 0.800320 | 1.523433e-48 | -8.615850e-47 |
| Msa0228070 | Msa0940380 | 0.804801 | 1.814228e-49 | -8.615850e-47 |
| Msa0481530 | Msa0940380 | 0.802028 | 6.813105e-49 | -8.615850e-47 |
| Msa0289250 | Msa0940380 | 0.801733 | 7.834318e-49 | -8.615850e-47 |
| Msa0940380 | Msa0969810 | 0.818162 | 2.263017e-52 | -8.615850e-47 |
| Msa0940380 | Msa1063510 | 0.801703 | 7.943660e-49 | -8.615850e-47 |
| Msa0940380 | Msa1077030 | 0.805598 | 1.235385e-49 | -8.615850e-47 |
| Msa0940380 | Msa1091940 | 0.807544 | 4.799293e-50 | -8.615850e-47 |
| Msa0940380 | Msa1169550 | 0.819817 | 9.516265e-53 | -8.615850e-47 |
| Msa0940380 | Msa1254730 | 0.807719 | 4.406258e-50 | -8.615850e-47 |
| Msa0940380 | Msa1294820 | 0.802735 | 4.873238e-49 | -8.615850e-47 |
| Msa0940380 | Msa1403340 | 0.800053 | 1.726158e-48 | -8.615850e-47 |
| Msa0940380 | Msa1410100 | 0.805969 | 1.032411e-49 | -8.615850e-47 |
| Msa0940380 | Msa1444310 | 0.802939 | 4.421705e-49 | -8.615850e-47 |
| Msa0940380 | Msa1466540 | 0.804308 | 2.299688e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGATCCTAAAACGATGTTT+AGG | 0.257955 | 6_3:-12751318 | None:intergenic |
| TACCAATGGGCACAACTATA+TGG | 0.314205 | 6_3:+12751271 | Msa0940370:intron |
| GACATTACTCTTTGGGTTGA+TGG | 0.319801 | 6_3:-12750983 | Msa0940380:three_prime_UTR |
| AACCAAGTGACATTACTCTT+TGG | 0.325367 | 6_3:-12750991 | Msa0940380:three_prime_UTR |
| ACCAAGTGACATTACTCTTT+GGG | 0.347851 | 6_3:-12750990 | Msa0940380:three_prime_UTR |
| TAGTAATCTTCATTGTCATC+TGG | 0.350490 | 6_3:-12750890 | Msa0940380:three_prime_UTR |
| AGATGAAGAGGAGGAGGAGG+AGG | 0.357794 | 6_3:-12751053 | Msa0940380:CDS |
| GTCAGGAGATATAGAGGAAG+AGG | 0.392327 | 6_3:-12751077 | Msa0940380:CDS |
| TGATCCTAAAACGATGTTTA+GGG | 0.416599 | 6_3:-12751317 | None:intergenic |
| GGAAGATGAAGAGGAGGAGG+AGG | 0.433178 | 6_3:-12751056 | Msa0940380:CDS |
| TTATAGGTCAGGAGATATAG+AGG | 0.452362 | 6_3:-12751083 | Msa0940380:intron |
| AGAGGAAGATGAAGAGGAGG+AGG | 0.452688 | 6_3:-12751059 | Msa0940380:CDS |
| AGTAATCTTCATTGTCATCT+GGG | 0.455340 | 6_3:-12750889 | Msa0940380:three_prime_UTR |
| AGAGGAAGAGGAAGATGAAG+AGG | 0.465081 | 6_3:-12751065 | Msa0940380:CDS |
| TACTCTTTGGGTTGATGGAG+TGG | 0.511257 | 6_3:-12750978 | Msa0940380:three_prime_UTR |
| AGCCATATAGTTGTGCCCAT+TGG | 0.523343 | 6_3:-12751273 | Msa0940380:intron |
| GGAAGAGGAAGATGAAGAGG+AGG | 0.525516 | 6_3:-12751062 | Msa0940380:CDS |
| GGAAGGGCATTCATTATGAA+AGG | 0.583957 | 6_3:-12751023 | Msa0940380:exon |
| ACCCAAAGAGTAATGTCACT+TGG | 0.593596 | 6_3:+12750989 | Msa0940370:intron |
| GCATTCATTATGAAAGGACG+AGG | 0.643179 | 6_3:-12751017 | Msa0940380:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATAAAATAAAAACACAAA+CGG | + | chr6_3:12751046-12751065 | Msa0940370:intron | 15.0% |
| !!! | ATATTTTTAAATTATAGGTC+AGG | - | chr6_3:12751098-12751117 | Msa0940380:intron | 15.0% |
| !! | AAATGAATGCATAATGATAA+TGG | + | chr6_3:12751020-12751039 | Msa0940370:intron | 20.0% |
| !!! | TATTTTATGTTGTGTATTGT+TGG | - | chr6_3:12751056-12751075 | Msa0940380:CDS | 20.0% |
| !! | TCTTTGTTTACTTACGAATA+TGG | - | chr6_3:12750985-12751004 | Msa0940380:three_prime_UTR | 25.0% |
| ! | CTACGATAAAAAAAGATAAC+AGG | - | chr6_3:12751265-12751284 | Msa0940380:intron | 25.0% |
| TGTTTACTTACGAATATGGA+TGG | - | chr6_3:12750989-12751008 | Msa0940380:three_prime_UTR | 30.0% | |
| TAGTAATCTTCATTGTCATC+TGG | - | chr6_3:12751302-12751321 | Msa0940380:CDS | 30.0% | |
| AGTAATCTTCATTGTCATCT+GGG | - | chr6_3:12751303-12751322 | Msa0940380:CDS | 30.0% | |
| AAATCAAAGCCTGTACCAAT+GGG | + | chr6_3:12750937-12750956 | Msa0940370:intron | 35.0% | |
| TTATAGGTCAGGAGATATAG+AGG | - | chr6_3:12751109-12751128 | Msa0940380:intron | 35.0% | |
| AACCAAGTGACATTACTCTT+TGG | - | chr6_3:12751201-12751220 | Msa0940380:intron | 35.0% | |
| ACCAAGTGACATTACTCTTT+GGG | - | chr6_3:12751202-12751221 | Msa0940380:intron | 35.0% | |
| TACCAATGGGCACAACTATA+TGG | + | chr6_3:12750924-12750943 | Msa0940370:intron | 40.0% | |
| CAAATCAAAGCCTGTACCAA+TGG | + | chr6_3:12750938-12750957 | Msa0940370:intron | 40.0% | |
| !! | TTGGTACAGGCTTTGATTTG+TGG | - | chr6_3:12750938-12750957 | Msa0940380:three_prime_UTR | 40.0% |
| !! | GGAAGGGCATTCATTATGAA+AGG | - | chr6_3:12751169-12751188 | Msa0940380:intron | 40.0% |
| GCATTCATTATGAAAGGACG+AGG | - | chr6_3:12751175-12751194 | Msa0940380:intron | 40.0% | |
| ACCCAAAGAGTAATGTCACT+TGG | + | chr6_3:12751206-12751225 | Msa0940370:intron | 40.0% | |
| !! | GACATTACTCTTTGGGTTGA+TGG | - | chr6_3:12751209-12751228 | Msa0940380:intron | 40.0% |
| AGCCATATAGTTGTGCCCAT+TGG | - | chr6_3:12750919-12750938 | Msa0940380:three_prime_UTR | 45.0% | |
| ATAGTTGTGCCCATTGGTAC+AGG | - | chr6_3:12750925-12750944 | Msa0940380:three_prime_UTR | 45.0% | |
| !! | GGCTTTGATTTGTGGCTTCA+TGG | - | chr6_3:12750946-12750965 | Msa0940380:three_prime_UTR | 45.0% |
| GTCAGGAGATATAGAGGAAG+AGG | - | chr6_3:12751115-12751134 | Msa0940380:intron | 45.0% | |
| AGAGGAAGAGGAAGATGAAG+AGG | - | chr6_3:12751127-12751146 | Msa0940380:intron | 45.0% | |
| !! | TACTCTTTGGGTTGATGGAG+TGG | - | chr6_3:12751214-12751233 | Msa0940380:intron | 45.0% |
| !!! | AATTGATATTTTTAAATTAT+AGG | - | chr6_3:12751093-12751112 | Msa0940380:intron | 5.0% |
| GGAAGAGGAAGATGAAGAGG+AGG | - | chr6_3:12751130-12751149 | Msa0940380:intron | 50.0% | |
| AGAGGAAGATGAAGAGGAGG+AGG | - | chr6_3:12751133-12751152 | Msa0940380:intron | 50.0% | |
| GGAAGATGAAGAGGAGGAGG+AGG | - | chr6_3:12751136-12751155 | Msa0940380:intron | 55.0% | |
| AGATGAAGAGGAGGAGGAGG+AGG | - | chr6_3:12751139-12751158 | Msa0940380:intron | 55.0% | |
| TGAAGAGGAGGAGGAGGAGG+AGG | - | chr6_3:12751142-12751161 | Msa0940380:intron | 60.0% | |
| AGGAGGAGGAGGAGGAGGAA+GGG | - | chr6_3:12751153-12751172 | Msa0940380:intron | 60.0% | |
| AGAGGAGGAGGAGGAGGAGG+AGG | - | chr6_3:12751145-12751164 | Msa0940380:intron | 65.0% | |
| GAGGAGGAGGAGGAGGAGGA+AGG | - | chr6_3:12751146-12751165 | Msa0940380:intron | 65.0% | |
| GGAGGAGGAGGAGGAGGAGG+AGG | - | chr6_3:12751148-12751167 | Msa0940380:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 12750888 | 12751326 | 12750888 | ID=Msa0940380;Name=Msa0940380 |
| chr6_3 | mRNA | 12750888 | 12751326 | 12750888 | ID=Msa0940380-mRNA-1;Parent=Msa0940380;Name=Msa0940380-mRNA-1;_AED=0.28;_eAED=0.28;_QI=0|0|0.5|0.5|1|1|2|139|41 |
| chr6_3 | exon | 12750888 | 12751099 | 12750888 | ID=Msa0940380-mRNA-1:exon:4185;Parent=Msa0940380-mRNA-1 |
| chr6_3 | exon | 12751274 | 12751326 | 12751274 | ID=Msa0940380-mRNA-1:exon:4184;Parent=Msa0940380-mRNA-1 |
| chr6_3 | CDS | 12751274 | 12751326 | 12751274 | ID=Msa0940380-mRNA-1:cds;Parent=Msa0940380-mRNA-1 |
| chr6_3 | CDS | 12751027 | 12751099 | 12751027 | ID=Msa0940380-mRNA-1:cds;Parent=Msa0940380-mRNA-1 |
| chr6_3 | three_prime_UTR | 12750888 | 12751026 | 12750888 | ID=Msa0940380-mRNA-1:three_prime_utr;Parent=Msa0940380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
