Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0950070 | KEH26022.1 | 92.593 | 81 | 5 | 1 | 1 | 80 | 1 | 81 | 2.82e-46 | 152 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0950070 | sp|Q941I0|ENOXC_FRAAN | 59.420 | 69 | 28 | 0 | 10 | 78 | 5 | 73 | 1.01e-22 | 91.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0643230 | Msa0950070 | 0.808382 | 3.183441e-50 | -8.615850e-47 |
| Msa0657710 | Msa0950070 | 0.802597 | 5.202821e-49 | -8.615850e-47 |
| Msa0702800 | Msa0950070 | 0.802665 | 5.037051e-49 | -8.615850e-47 |
| Msa0828960 | Msa0950070 | 0.804321 | 2.284411e-49 | -8.615850e-47 |
| Msa0950070 | Msa1242160 | 0.804911 | 1.721008e-49 | -8.615850e-47 |
| Msa0950070 | Msa1283930 | 0.805845 | 1.096754e-49 | -8.615850e-47 |
| Msa0950070 | Msa1319010 | 0.801427 | 9.051500e-49 | -8.615850e-47 |
| Msa0950070 | Msa1322960 | 0.806052 | 9.918171e-50 | -8.615850e-47 |
| Msa0950070 | Msa1445530 | 0.812376 | 4.373851e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0950070 | MtrunA17_Chr6g0465551 | 92.593 | 81 | 5 | 1 | 1 | 80 | 1 | 81 | 3.76e-50 | 152 |
| Msa0950070 | MtrunA17_Chr6g0465501 | 91.358 | 81 | 6 | 1 | 1 | 80 | 1 | 81 | 1.58e-48 | 155 |
| Msa0950070 | MtrunA17_Chr6g0466321 | 91.358 | 81 | 6 | 1 | 1 | 80 | 1 | 81 | 2.85e-48 | 155 |
| Msa0950070 | MtrunA17_Chr7g0228011 | 63.077 | 65 | 24 | 0 | 16 | 80 | 6 | 70 | 4.65e-23 | 89.4 |
| Msa0950070 | MtrunA17_Chr6g0453201 | 55.714 | 70 | 31 | 0 | 11 | 80 | 4 | 73 | 3.37e-18 | 76.3 |
| Msa0950070 | MtrunA17_Chr6g0453191 | 54.412 | 68 | 31 | 0 | 13 | 80 | 6 | 73 | 5.40e-18 | 75.5 |
| Msa0950070 | MtrunA17_Chr8g0365421 | 50.000 | 78 | 37 | 2 | 2 | 78 | 59 | 135 | 2.13e-17 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0950070 | AT1G23740.1 | 57.576 | 66 | 27 | 1 | 13 | 78 | 73 | 137 | 2.82e-18 | 77.4 |
Find 22 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGTAATCCACTGGATTA+AGG | 0.225202 | 6_3:-37702237 | None:intergenic |
| TTCTAAAGTTACATCCAAAT+TGG | 0.322662 | 6_3:+37702161 | Msa0950070:CDS |
| AGTTGAGGTATGGACCAATT+TGG | 0.333043 | 6_3:-37702175 | None:intergenic |
| CTTGTAATCCACTGGATTAA+GGG | 0.388316 | 6_3:-37702236 | None:intergenic |
| GTCATCTTTGAGTTGAGGTA+TGG | 0.412969 | 6_3:-37702185 | None:intergenic |
| TGTAACTTTAGAATATCTAA+TGG | 0.437701 | 6_3:-37702151 | None:intergenic |
| AAAATTAACATACAGGAAGA+TGG | 0.462364 | 6_3:-37702291 | None:intergenic |
| TGCATCCTCTTGTAATCCAC+TGG | 0.474314 | 6_3:-37702244 | None:intergenic |
| GAGGATGCATGCCTTGTTCA+AGG | 0.475670 | 6_3:+37702258 | Msa0950070:CDS |
| TGAGTTGGAATAGTAATAGT+TGG | 0.480302 | 6_3:-37702094 | None:intergenic |
| TAAAGAGAAAATTAACATAC+AGG | 0.487183 | 6_3:-37702298 | None:intergenic |
| TGCTGCTTCCCTTAATCCAG+TGG | 0.491910 | 6_3:+37702228 | Msa0950070:CDS |
| TTCCAACTCATACAAAAGCT+TGG | 0.500152 | 6_3:+37702107 | Msa0950070:CDS |
| CAACAACCTTGATGAGGACT+TGG | 0.502267 | 6_3:-37702207 | None:intergenic |
| AAATTAACATACAGGAAGAT+GGG | 0.515069 | 6_3:-37702290 | None:intergenic |
| ACTTGGTCATCTTTGAGTTG+AGG | 0.531211 | 6_3:-37702190 | None:intergenic |
| GCTTGGTTCTACTCTGAACA+TGG | 0.593786 | 6_3:+37702124 | Msa0950070:CDS |
| GGGATCAGTCTCCTTGAACA+AGG | 0.614193 | 6_3:-37702269 | None:intergenic |
| AGATGACCAAGTCCTCATCA+AGG | 0.619767 | 6_3:+37702201 | Msa0950070:CDS |
| AATTAACATACAGGAAGATG+GGG | 0.621275 | 6_3:-37702289 | None:intergenic |
| TTAATCCAGTGGATTACAAG+AGG | 0.638046 | 6_3:+37702239 | Msa0950070:CDS |
| AAGCAGCAACAACCTTGATG+AGG | 0.675221 | 6_3:-37702213 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTAACTTTAGAATATCTAA+TGG | - | chr6_3:37702154-37702173 | None:intergenic | 20.0% |
| !!! | TTCTAAAGTTACATCCAAAT+TGG | + | chr6_3:37702161-37702180 | Msa0950070:CDS | 25.0% |
| ! | TGAGTTGGAATAGTAATAGT+TGG | - | chr6_3:37702097-37702116 | None:intergenic | 30.0% |
| TTCCAACTCATACAAAAGCT+TGG | + | chr6_3:37702107-37702126 | Msa0950070:CDS | 35.0% | |
| ! | AACCAAGCTTTTGTATGAGT+TGG | - | chr6_3:37702112-37702131 | None:intergenic | 35.0% |
| CTTGTAATCCACTGGATTAA+GGG | - | chr6_3:37702239-37702258 | None:intergenic | 35.0% | |
| TCTTGTAATCCACTGGATTA+AGG | - | chr6_3:37702240-37702259 | None:intergenic | 35.0% | |
| !! | TTAATCCAGTGGATTACAAG+AGG | + | chr6_3:37702239-37702258 | Msa0950070:CDS | 35.0% |
| AGTTGAGGTATGGACCAATT+TGG | - | chr6_3:37702178-37702197 | None:intergenic | 40.0% | |
| !! | GTCATCTTTGAGTTGAGGTA+TGG | - | chr6_3:37702188-37702207 | None:intergenic | 40.0% |
| !! | ACTTGGTCATCTTTGAGTTG+AGG | - | chr6_3:37702193-37702212 | None:intergenic | 40.0% |
| !! | GCTTGGTTCTACTCTGAACA+TGG | + | chr6_3:37702124-37702143 | Msa0950070:CDS | 45.0% |
| AGATGACCAAGTCCTCATCA+AGG | + | chr6_3:37702201-37702220 | Msa0950070:CDS | 45.0% | |
| CAACAACCTTGATGAGGACT+TGG | - | chr6_3:37702210-37702229 | None:intergenic | 45.0% | |
| AAGCAGCAACAACCTTGATG+AGG | - | chr6_3:37702216-37702235 | None:intergenic | 45.0% | |
| TGCATCCTCTTGTAATCCAC+TGG | - | chr6_3:37702247-37702266 | None:intergenic | 45.0% | |
| ! | TGCTGCTTCCCTTAATCCAG+TGG | + | chr6_3:37702228-37702247 | Msa0950070:CDS | 50.0% |
| ! | GAGGATGCATGCCTTGTTCA+AGG | + | chr6_3:37702258-37702277 | Msa0950070:CDS | 50.0% |
| GGGATCAGTCTCCTTGAACA+AGG | - | chr6_3:37702272-37702291 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_3 | gene | 37702067 | 37702309 | 37702067 | ID=Msa0950070;Name=Msa0950070 |
| chr6_3 | mRNA | 37702067 | 37702309 | 37702067 | ID=Msa0950070-mRNA-1;Parent=Msa0950070;Name=Msa0950070-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|80 |
| chr6_3 | exon | 37702067 | 37702309 | 37702067 | ID=Msa0950070-mRNA-1:exon:9248;Parent=Msa0950070-mRNA-1 |
| chr6_3 | CDS | 37702067 | 37702309 | 37702067 | ID=Msa0950070-mRNA-1:cds;Parent=Msa0950070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
