AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0967750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0967750 MtrunA17_Chr1g0213011 98.198 222 4 0 1 222 1 222 1.01e-161 445
Msa0967750 MtrunA17_Chr1g0213021 62.896 221 82 0 2 222 5 225 7.70e-98 284
Msa0967750 MtrunA17_Chr1g0213031 61.187 219 85 0 2 220 5 223 8.08e-93 271
Msa0967750 MtrunA17_Chr1g0213041 54.884 215 95 1 4 216 5 219 1.75e-80 240
Msa0967750 MtrunA17_Chr1g0213051 52.804 214 99 1 4 215 5 218 7.94e-78 233
Msa0967750 MtrunA17_Chr7g0224381 50.000 162 81 0 4 165 5 166 4.81e-56 176
Msa0967750 MtrunA17_Chr3g0128761 32.086 187 116 5 29 209 15 196 1.83e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0967750 AT3G55120.1 49.048 210 107 0 4 213 15 224 3.16e-67 207
Msa0967750 AT5G66230.2 42.453 212 114 1 4 215 5 208 1.27e-53 180
Msa0967750 AT5G66220.1 42.767 159 91 0 57 215 1 159 5.51e-44 145
Msa0967750 AT5G05270.1 28.708 209 142 4 8 214 6 209 6.90e-13 65.5
Msa0967750 AT5G05270.2 28.708 209 142 4 8 214 6 209 6.90e-13 65.5

Find 80 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGACCAATTCACCTATTT+TGG 0.093565 6_3:-97849848 None:intergenic
GCTAATGATGTTTCAGATTT+TGG 0.097836 6_3:-97849960 None:intergenic
CAATCACCTGATGGAATATT+AGG 0.209322 6_3:+97850961 Msa0967750:CDS
CACTGCCATAGGTGTTTATT+TGG 0.211608 6_3:+97850150 Msa0967750:CDS
TTGAAATCAGTTGGAACTTA+TGG 0.230001 6_3:+97850853 Msa0967750:CDS
TTCTTGTGCTGCAACGCTTT+TGG 0.248420 6_3:-97851331 None:intergenic
AATTAGAGGGTCAAAGATTA+GGG 0.310626 6_3:+97850777 Msa0967750:CDS
CTTGATGAAGTTTCCTTCAA+TGG 0.330932 6_3:-97850125 None:intergenic
CCCTGATCTTAAGCGCTGTT+TGG 0.332738 6_3:+97851225 Msa0967750:CDS
AATAATTAAGAACAAGAATA+TGG 0.334406 6_3:+97849991 Msa0967750:exon
AACGAGGGTGCTTTCAAGAT+TGG 0.337469 6_3:+97851271 Msa0967750:CDS
TAATTAGAGGGTCAAAGATT+AGG 0.343487 6_3:+97850776 Msa0967750:CDS
AAAATCCAAAATAGGTGAAT+TGG 0.349824 6_3:+97849843 Msa0967750:five_prime_UTR
TCATATTTCCTCGGTGGCGC+TGG 0.355424 6_3:+97850088 Msa0967750:CDS
CCCTTTGAAAAGTTAATTAG+AGG 0.361486 6_3:+97850763 Msa0967750:CDS
AATCACCTGATGGAATATTA+GGG 0.365814 6_3:+97850962 Msa0967750:CDS
TCAAATAAAATACATGCAAC+TGG 0.398245 6_3:+97849866 Msa0967750:five_prime_UTR
GCCAAACAGCGCTTAAGATC+AGG 0.410390 6_3:-97851226 None:intergenic
GCACCAGGTGGAAAATTAAC+AGG 0.410631 6_3:-97850922 None:intergenic
ACCGAGGAAATATGATTTGC+CGG 0.416952 6_3:-97850080 None:intergenic
AGATGATTTACCCTTCCATT+TGG 0.439741 6_3:-97850200 None:intergenic
ACTTGCGTTAAGTGAAGAAC+GGG 0.443654 6_3:-97849932 None:intergenic
GGCAGTTTCATCAGCAGTGT+TGG 0.459798 6_3:+97851174 Msa0967750:CDS
CTCGGTGGCGCTGGGGAGAG+AGG 0.461142 6_3:+97850097 Msa0967750:CDS
CATATTTCCTCGGTGGCGCT+GGG 0.461249 6_3:+97850089 Msa0967750:CDS
GGAACGAAATAAACGCCGCC+TGG 0.467910 6_3:+97849887 Msa0967750:five_prime_UTR
AAATATGATTTGCCGGTGAC+CGG 0.472992 6_3:-97850073 None:intergenic
ACCGGAGAGGTAACCACCGC+TGG 0.477165 6_3:-97850055 None:intergenic
TGAGTACTCAAGGAAGGTTA+TGG 0.487878 6_3:+97850813 Msa0967750:CDS
TAACCACCGCTGGGTATTCA+AGG 0.488480 6_3:-97850045 None:intergenic
GGATACAAGTATACCAGAAA+AGG 0.490457 6_3:+97851129 Msa0967750:CDS
AATAGGAAGCGGTCTGGCCC+AGG 0.492465 6_3:-97849905 None:intergenic
CCCAGCGGTGGTTACCTCTC+CGG 0.492838 6_3:+97850054 Msa0967750:CDS
TATTATTGATACTAATAAGA+AGG 0.499753 6_3:-97851408 None:intergenic
TGGCTTCACTAGCTGCCAAA+TGG 0.503292 6_3:+97850185 Msa0967750:CDS
GTTACAGCTTAGTTTCTCTC+CGG 0.504378 6_3:+97851108 Msa0967750:intron
GAGAGAGGATTGACCATTGA+AGG 0.509487 6_3:+97850112 Msa0967750:CDS
CTAACCCCTAATATTCCATC+AGG 0.511312 6_3:-97850967 None:intergenic
ATCTTCCAAATAAACACCTA+TGG 0.512141 6_3:-97850155 None:intergenic
GTGGTTACCTCTCCGGTCAC+CGG 0.524005 6_3:+97850061 Msa0967750:CDS
TTCACTAGCTGCCAAATGGA+AGG 0.526249 6_3:+97850189 Msa0967750:CDS
CCAAACAGCGCTTAAGATCA+GGG 0.532079 6_3:-97851225 None:intergenic
TTCTACAGGCAATCACCTGA+TGG 0.554083 6_3:+97850952 Msa0967750:CDS
GAACGAAATAAACGCCGCCT+GGG 0.554703 6_3:+97849888 Msa0967750:five_prime_UTR
GACTTGCGTTAAGTGAAGAA+CGG 0.560970 6_3:-97849933 None:intergenic
GGCTGCACTCATAGAGAACA+AGG 0.569164 6_3:+97851153 Msa0967750:CDS
ACCGGCAAATCATATTTCCT+CGG 0.576452 6_3:+97850079 Msa0967750:CDS
TGAGTGGTCCTGAGTACTCA+AGG 0.577740 6_3:+97850803 Msa0967750:CDS
CTCTCTCCCCAGCGCCACCG+AGG 0.582911 6_3:-97850096 None:intergenic
CTGTAGAAAACAGAGGCACC+AGG 0.591901 6_3:-97850937 None:intergenic
CCTTTGAAAAGTTAATTAGA+GGG 0.595496 6_3:+97850764 Msa0967750:CDS
ATTACCTGCGTTGTTGAACG+AGG 0.600190 6_3:+97851255 Msa0967750:CDS
TGATGTCTCTGTAAAAGTCG+AGG 0.602205 6_3:-97850240 None:intergenic
ATAACCTTCCTTGAGTACTC+AGG 0.607676 6_3:-97850811 None:intergenic
TTCATCAAGTTCACTGCCAT+AGG 0.610295 6_3:+97850139 Msa0967750:CDS
GCAGTGTTGGAGACTATGAT+CGG 0.622152 6_3:+97851187 Msa0967750:CDS
AGGAAGCGGTCTGGCCCAGG+CGG 0.623344 6_3:-97849902 None:intergenic
TCACTAGCTGCCAAATGGAA+GGG 0.624312 6_3:+97850190 Msa0967750:CDS
GTGGCACACTTGAAATCAGT+TGG 0.625898 6_3:+97850844 Msa0967750:CDS
GATGTCTCTGTAAAAGTCGA+GGG 0.629213 6_3:-97850239 None:intergenic
CCGGAGAGGTAACCACCGCT+GGG 0.632340 6_3:-97850054 None:intergenic
TACAAGTATACCAGAAAAGG+AGG 0.632960 6_3:+97851132 Msa0967750:CDS
TGGTCCTGAGTACTCAAGGA+AGG 0.639707 6_3:+97850807 Msa0967750:CDS
GGCAAATCATATTTCCTCGG+TGG 0.648065 6_3:+97850082 Msa0967750:CDS
AAGGTTCTCCACAGTGATTG+CGG 0.648293 6_3:-97850026 None:intergenic
ATATTTCCTCGGTGGCGCTG+GGG 0.654176 6_3:+97850090 Msa0967750:CDS
GCACCCTCGTTCAACAACGC+AGG 0.670342 6_3:-97851259 None:intergenic
TGATTTGCCGGTGACCGGAG+AGG 0.671162 6_3:-97850068 None:intergenic
TTATTTGGAAGATATAGCAG+TGG 0.671898 6_3:+97850165 Msa0967750:CDS
GAACCTTGAATACCCAGCGG+TGG 0.677026 6_3:+97850042 Msa0967750:CDS
CAAACAGCGCTTAAGATCAG+GGG 0.682290 6_3:-97851224 None:intergenic
TTACCTGCGTTGTTGAACGA+GGG 0.686961 6_3:+97851256 Msa0967750:CDS
ATCACCTGATGGAATATTAG+GGG 0.689762 6_3:+97850963 Msa0967750:CDS
TCAAAGATTAGGGAATTGAG+TGG 0.698363 6_3:+97850787 Msa0967750:CDS
TAGAAAACAGAGGCACCAGG+TGG 0.700225 6_3:-97850934 None:intergenic
GCAGCACAAGAATGAGACCA+TGG 0.722638 6_3:+97851342 Msa0967750:three_prime_UTR
ATCAATCACCGCAATCACTG+TGG 0.724881 6_3:+97850018 Msa0967750:CDS
GAAGGTTATGGAGAACTGTG+TGG 0.734369 6_3:+97850825 Msa0967750:CDS
TGATTGCCTGTAGAAAACAG+AGG 0.767471 6_3:-97850944 None:intergenic
GGAGAACCTTGAATACCCAG+CGG 0.782943 6_3:+97850039 Msa0967750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTAAGAACAAGAATA+TGG + chr6_3:97849991-97850010 Msa0967750:exon 15.0%
!! AAATCATTTAATTAATTGCA+AGG - chr6_3:97850306-97850325 None:intergenic 15.0%
!! GTAATACTATAGTATTTAAT+TGG - chr6_3:97850622-97850641 None:intergenic 15.0%
!!! TGATAAATTGATAATTTTGT+AGG + chr6_3:97850739-97850758 Msa0967750:intron 15.0%
!!! TTTTAATATACTAGTATTAG+TGG - chr6_3:97851082-97851101 None:intergenic 15.0%
!! TATTATTGATACTAATAAGA+AGG - chr6_3:97851411-97851430 None:intergenic 15.0%
!! AATAATTAAGAACAAGAATA+TGG + chr6_3:97849991-97850010 Msa0967750:exon 15.0%
!! AAATCATTTAATTAATTGCA+AGG - chr6_3:97850306-97850325 None:intergenic 15.0%
!! GTAATACTATAGTATTTAAT+TGG - chr6_3:97850622-97850641 None:intergenic 15.0%
!!! TGATAAATTGATAATTTTGT+AGG + chr6_3:97850739-97850758 Msa0967750:intron 15.0%
!!! TTTTAATATACTAGTATTAG+TGG - chr6_3:97851082-97851101 None:intergenic 15.0%
!! TATTATTGATACTAATAAGA+AGG - chr6_3:97851411-97851430 None:intergenic 15.0%
!! AGAAAAGAAAAATCCAAAAT+AGG + chr6_3:97849835-97849854 Msa0967750:five_prime_UTR 20.0%
!! GTTAGACTTTAATTAATTCT+TGG - chr6_3:97850531-97850550 None:intergenic 20.0%
!! AATTAAAGTCTAACATACAA+CGG + chr6_3:97850537-97850556 Msa0967750:intron 20.0%
!! AGTTAAGAAACAGAATTAAT+TGG - chr6_3:97851004-97851023 None:intergenic 20.0%
!!! TTTCTTAACTATTTTAGTTG+AGG + chr6_3:97851014-97851033 Msa0967750:intron 20.0%
!! AGAAAAGAAAAATCCAAAAT+AGG + chr6_3:97849835-97849854 Msa0967750:five_prime_UTR 20.0%
!! GTTAGACTTTAATTAATTCT+TGG - chr6_3:97850531-97850550 None:intergenic 20.0%
!! AATTAAAGTCTAACATACAA+CGG + chr6_3:97850537-97850556 Msa0967750:intron 20.0%
!! AGTTAAGAAACAGAATTAAT+TGG - chr6_3:97851004-97851023 None:intergenic 20.0%
!!! TTTCTTAACTATTTTAGTTG+AGG + chr6_3:97851014-97851033 Msa0967750:intron 20.0%
! AAAATCCAAAATAGGTGAAT+TGG + chr6_3:97849843-97849862 Msa0967750:five_prime_UTR 25.0%
! TCAAATAAAATACATGCAAC+TGG + chr6_3:97849866-97849885 Msa0967750:five_prime_UTR 25.0%
!!! CATTTTTTAACATATGAAGC+TGG + chr6_3:97850361-97850380 Msa0967750:intron 25.0%
! AATGTGAGTAAAAACTATGA+AGG - chr6_3:97850430-97850449 None:intergenic 25.0%
! TCAATAAGACTCATATGATT+TGG - chr6_3:97850496-97850515 None:intergenic 25.0%
! TTTAATTGGATGAATTCAAG+TGG - chr6_3:97850608-97850627 None:intergenic 25.0%
!! CCTCTAATTAACTTTTCAAA+GGG - chr6_3:97850766-97850785 None:intergenic 25.0%
!! CCTTTGAAAAGTTAATTAGA+GGG + chr6_3:97850764-97850783 Msa0967750:CDS 25.0%
! ATATACTAGTATTAGTGGAA+AGG - chr6_3:97851077-97851096 None:intergenic 25.0%
! AAAATCCAAAATAGGTGAAT+TGG + chr6_3:97849843-97849862 Msa0967750:five_prime_UTR 25.0%
! TCAAATAAAATACATGCAAC+TGG + chr6_3:97849866-97849885 Msa0967750:five_prime_UTR 25.0%
!!! CATTTTTTAACATATGAAGC+TGG + chr6_3:97850361-97850380 Msa0967750:intron 25.0%
! AATGTGAGTAAAAACTATGA+AGG - chr6_3:97850430-97850449 None:intergenic 25.0%
! TCAATAAGACTCATATGATT+TGG - chr6_3:97850496-97850515 None:intergenic 25.0%
! TTTAATTGGATGAATTCAAG+TGG - chr6_3:97850608-97850627 None:intergenic 25.0%
!! CCTCTAATTAACTTTTCAAA+GGG - chr6_3:97850766-97850785 None:intergenic 25.0%
!! CCTTTGAAAAGTTAATTAGA+GGG + chr6_3:97850764-97850783 Msa0967750:CDS 25.0%
! ATATACTAGTATTAGTGGAA+AGG - chr6_3:97851077-97851096 None:intergenic 25.0%
! TTTGACCAATTCACCTATTT+TGG - chr6_3:97849851-97849870 None:intergenic 30.0%
! GCTAATGATGTTTCAGATTT+TGG - chr6_3:97849963-97849982 None:intergenic 30.0%
ATCTTCCAAATAAACACCTA+TGG - chr6_3:97850158-97850177 None:intergenic 30.0%
TTATTTGGAAGATATAGCAG+TGG + chr6_3:97850165-97850184 Msa0967750:CDS 30.0%
!! CTCATATGATTTGGTTGATT+AGG - chr6_3:97850487-97850506 None:intergenic 30.0%
!! AGTCTTATTGATGTAGAGTT+CGG + chr6_3:97850505-97850524 Msa0967750:intron 30.0%
! CCCTTTGAAAAGTTAATTAG+AGG + chr6_3:97850763-97850782 Msa0967750:CDS 30.0%
! CCCTCTAATTAACTTTTCAA+AGG - chr6_3:97850767-97850786 None:intergenic 30.0%
TAATTAGAGGGTCAAAGATT+AGG + chr6_3:97850776-97850795 Msa0967750:CDS 30.0%
AATTAGAGGGTCAAAGATTA+GGG + chr6_3:97850777-97850796 Msa0967750:CDS 30.0%
TTGAAATCAGTTGGAACTTA+TGG + chr6_3:97850853-97850872 Msa0967750:CDS 30.0%
AATCACCTGATGGAATATTA+GGG + chr6_3:97850962-97850981 Msa0967750:CDS 30.0%
!! TTTTCTGGTATACTTGTATC+CGG - chr6_3:97851130-97851149 None:intergenic 30.0%
TGTAGACTTAAAAAAGTCCA+TGG - chr6_3:97851362-97851381 None:intergenic 30.0%
! TTTGACCAATTCACCTATTT+TGG - chr6_3:97849851-97849870 None:intergenic 30.0%
! GCTAATGATGTTTCAGATTT+TGG - chr6_3:97849963-97849982 None:intergenic 30.0%
ATCTTCCAAATAAACACCTA+TGG - chr6_3:97850158-97850177 None:intergenic 30.0%
TTATTTGGAAGATATAGCAG+TGG + chr6_3:97850165-97850184 Msa0967750:CDS 30.0%
!! CTCATATGATTTGGTTGATT+AGG - chr6_3:97850487-97850506 None:intergenic 30.0%
!! AGTCTTATTGATGTAGAGTT+CGG + chr6_3:97850505-97850524 Msa0967750:intron 30.0%
! CCCTTTGAAAAGTTAATTAG+AGG + chr6_3:97850763-97850782 Msa0967750:CDS 30.0%
! CCCTCTAATTAACTTTTCAA+AGG - chr6_3:97850767-97850786 None:intergenic 30.0%
TAATTAGAGGGTCAAAGATT+AGG + chr6_3:97850776-97850795 Msa0967750:CDS 30.0%
AATTAGAGGGTCAAAGATTA+GGG + chr6_3:97850777-97850796 Msa0967750:CDS 30.0%
TTGAAATCAGTTGGAACTTA+TGG + chr6_3:97850853-97850872 Msa0967750:CDS 30.0%
AATCACCTGATGGAATATTA+GGG + chr6_3:97850962-97850981 Msa0967750:CDS 30.0%
!! TTTTCTGGTATACTTGTATC+CGG - chr6_3:97851130-97851149 None:intergenic 30.0%
TGTAGACTTAAAAAAGTCCA+TGG - chr6_3:97851362-97851381 None:intergenic 30.0%
!! AGTGAAGAACGGGTTTTAAT+AGG - chr6_3:97849925-97849944 None:intergenic 35.0%
CTTGATGAAGTTTCCTTCAA+TGG - chr6_3:97850128-97850147 None:intergenic 35.0%
AGATGATTTACCCTTCCATT+TGG - chr6_3:97850203-97850222 None:intergenic 35.0%
! TTTTACAGAGACATCATCTC+AGG + chr6_3:97850247-97850266 Msa0967750:CDS 35.0%
TCAAAGATTAGGGAATTGAG+TGG + chr6_3:97850787-97850806 Msa0967750:CDS 35.0%
CAATCACCTGATGGAATATT+AGG + chr6_3:97850961-97850980 Msa0967750:CDS 35.0%
ATCACCTGATGGAATATTAG+GGG + chr6_3:97850963-97850982 Msa0967750:CDS 35.0%
GGATACAAGTATACCAGAAA+AGG + chr6_3:97851129-97851148 Msa0967750:CDS 35.0%
TACAAGTATACCAGAAAAGG+AGG + chr6_3:97851132-97851151 Msa0967750:CDS 35.0%
!! AGTGAAGAACGGGTTTTAAT+AGG - chr6_3:97849925-97849944 None:intergenic 35.0%
CTTGATGAAGTTTCCTTCAA+TGG - chr6_3:97850128-97850147 None:intergenic 35.0%
AGATGATTTACCCTTCCATT+TGG - chr6_3:97850203-97850222 None:intergenic 35.0%
! TTTTACAGAGACATCATCTC+AGG + chr6_3:97850247-97850266 Msa0967750:CDS 35.0%
TCAAAGATTAGGGAATTGAG+TGG + chr6_3:97850787-97850806 Msa0967750:CDS 35.0%
CAATCACCTGATGGAATATT+AGG + chr6_3:97850961-97850980 Msa0967750:CDS 35.0%
ATCACCTGATGGAATATTAG+GGG + chr6_3:97850963-97850982 Msa0967750:CDS 35.0%
GGATACAAGTATACCAGAAA+AGG + chr6_3:97851129-97851148 Msa0967750:CDS 35.0%
TACAAGTATACCAGAAAAGG+AGG + chr6_3:97851132-97851151 Msa0967750:CDS 35.0%
!! GAACGGGTTTTAATAGGAAG+CGG - chr6_3:97849919-97849938 None:intergenic 40.0%
ACTTGCGTTAAGTGAAGAAC+GGG - chr6_3:97849935-97849954 None:intergenic 40.0%
GACTTGCGTTAAGTGAAGAA+CGG - chr6_3:97849936-97849955 None:intergenic 40.0%
! AAATATGATTTGCCGGTGAC+CGG - chr6_3:97850076-97850095 None:intergenic 40.0%
ACCGGCAAATCATATTTCCT+CGG + chr6_3:97850079-97850098 Msa0967750:CDS 40.0%
! ACCGAGGAAATATGATTTGC+CGG - chr6_3:97850083-97850102 None:intergenic 40.0%
TTCATCAAGTTCACTGCCAT+AGG + chr6_3:97850139-97850158 Msa0967750:CDS 40.0%
CACTGCCATAGGTGTTTATT+TGG + chr6_3:97850150-97850169 Msa0967750:CDS 40.0%
GATGTCTCTGTAAAAGTCGA+GGG - chr6_3:97850242-97850261 None:intergenic 40.0%
TGATGTCTCTGTAAAAGTCG+AGG - chr6_3:97850243-97850262 None:intergenic 40.0%
ATAACCTTCCTTGAGTACTC+AGG - chr6_3:97850814-97850833 None:intergenic 40.0%
TGAGTACTCAAGGAAGGTTA+TGG + chr6_3:97850813-97850832 Msa0967750:CDS 40.0%
! AAGCCTGTTAATTTTCCACC+TGG + chr6_3:97850919-97850938 Msa0967750:CDS 40.0%
TGATTGCCTGTAGAAAACAG+AGG - chr6_3:97850947-97850966 None:intergenic 40.0%
CTAACCCCTAATATTCCATC+AGG - chr6_3:97850970-97850989 None:intergenic 40.0%
GTTACAGCTTAGTTTCTCTC+CGG + chr6_3:97851108-97851127 Msa0967750:intron 40.0%
TGAGTAGAGTTGCTTAACTG+AGG + chr6_3:97851564-97851583 Msa0967750:three_prime_UTR 40.0%
!! GAACGGGTTTTAATAGGAAG+CGG - chr6_3:97849919-97849938 None:intergenic 40.0%
ACTTGCGTTAAGTGAAGAAC+GGG - chr6_3:97849935-97849954 None:intergenic 40.0%
GACTTGCGTTAAGTGAAGAA+CGG - chr6_3:97849936-97849955 None:intergenic 40.0%
! AAATATGATTTGCCGGTGAC+CGG - chr6_3:97850076-97850095 None:intergenic 40.0%
ACCGGCAAATCATATTTCCT+CGG + chr6_3:97850079-97850098 Msa0967750:CDS 40.0%
! ACCGAGGAAATATGATTTGC+CGG - chr6_3:97850083-97850102 None:intergenic 40.0%
TTCATCAAGTTCACTGCCAT+AGG + chr6_3:97850139-97850158 Msa0967750:CDS 40.0%
CACTGCCATAGGTGTTTATT+TGG + chr6_3:97850150-97850169 Msa0967750:CDS 40.0%
GATGTCTCTGTAAAAGTCGA+GGG - chr6_3:97850242-97850261 None:intergenic 40.0%
TGATGTCTCTGTAAAAGTCG+AGG - chr6_3:97850243-97850262 None:intergenic 40.0%
ATAACCTTCCTTGAGTACTC+AGG - chr6_3:97850814-97850833 None:intergenic 40.0%
TGAGTACTCAAGGAAGGTTA+TGG + chr6_3:97850813-97850832 Msa0967750:CDS 40.0%
! AAGCCTGTTAATTTTCCACC+TGG + chr6_3:97850919-97850938 Msa0967750:CDS 40.0%
TGATTGCCTGTAGAAAACAG+AGG - chr6_3:97850947-97850966 None:intergenic 40.0%
CTAACCCCTAATATTCCATC+AGG - chr6_3:97850970-97850989 None:intergenic 40.0%
GTTACAGCTTAGTTTCTCTC+CGG + chr6_3:97851108-97851127 Msa0967750:intron 40.0%
!! GGTTTTAATAGGAAGCGGTC+TGG - chr6_3:97849914-97849933 None:intergenic 45.0%
ATCAATCACCGCAATCACTG+TGG + chr6_3:97850018-97850037 Msa0967750:CDS 45.0%
AAGGTTCTCCACAGTGATTG+CGG - chr6_3:97850029-97850048 None:intergenic 45.0%
GGCAAATCATATTTCCTCGG+TGG + chr6_3:97850082-97850101 Msa0967750:CDS 45.0%
GAGAGAGGATTGACCATTGA+AGG + chr6_3:97850112-97850131 Msa0967750:CDS 45.0%
TTCACTAGCTGCCAAATGGA+AGG + chr6_3:97850189-97850208 Msa0967750:CDS 45.0%
TCACTAGCTGCCAAATGGAA+GGG + chr6_3:97850190-97850209 Msa0967750:CDS 45.0%
GACAGACACTCTTCAATCCT+CGG - chr6_3:97850408-97850427 None:intergenic 45.0%
GAAGGTTATGGAGAACTGTG+TGG + chr6_3:97850825-97850844 Msa0967750:CDS 45.0%
GTGGCACACTTGAAATCAGT+TGG + chr6_3:97850844-97850863 Msa0967750:CDS 45.0%
GCACCAGGTGGAAAATTAAC+AGG - chr6_3:97850925-97850944 None:intergenic 45.0%
TTCTACAGGCAATCACCTGA+TGG + chr6_3:97850952-97850971 Msa0967750:CDS 45.0%
! GCAGTGTTGGAGACTATGAT+CGG + chr6_3:97851187-97851206 Msa0967750:CDS 45.0%
CAAACAGCGCTTAAGATCAG+GGG - chr6_3:97851227-97851246 None:intergenic 45.0%
CCAAACAGCGCTTAAGATCA+GGG - chr6_3:97851228-97851247 None:intergenic 45.0%
ATTACCTGCGTTGTTGAACG+AGG + chr6_3:97851255-97851274 Msa0967750:CDS 45.0%
TTACCTGCGTTGTTGAACGA+GGG + chr6_3:97851256-97851275 Msa0967750:CDS 45.0%
!! AACGAGGGTGCTTTCAAGAT+TGG + chr6_3:97851271-97851290 Msa0967750:CDS 45.0%
! TTCTTGTGCTGCAACGCTTT+TGG - chr6_3:97851334-97851353 None:intergenic 45.0%
!! GGTTTTAATAGGAAGCGGTC+TGG - chr6_3:97849914-97849933 None:intergenic 45.0%
ATCAATCACCGCAATCACTG+TGG + chr6_3:97850018-97850037 Msa0967750:CDS 45.0%
AAGGTTCTCCACAGTGATTG+CGG - chr6_3:97850029-97850048 None:intergenic 45.0%
GGCAAATCATATTTCCTCGG+TGG + chr6_3:97850082-97850101 Msa0967750:CDS 45.0%
GAGAGAGGATTGACCATTGA+AGG + chr6_3:97850112-97850131 Msa0967750:CDS 45.0%
TTCACTAGCTGCCAAATGGA+AGG + chr6_3:97850189-97850208 Msa0967750:CDS 45.0%
TCACTAGCTGCCAAATGGAA+GGG + chr6_3:97850190-97850209 Msa0967750:CDS 45.0%
GACAGACACTCTTCAATCCT+CGG - chr6_3:97850408-97850427 None:intergenic 45.0%
GAAGGTTATGGAGAACTGTG+TGG + chr6_3:97850825-97850844 Msa0967750:CDS 45.0%
GTGGCACACTTGAAATCAGT+TGG + chr6_3:97850844-97850863 Msa0967750:CDS 45.0%
GCACCAGGTGGAAAATTAAC+AGG - chr6_3:97850925-97850944 None:intergenic 45.0%
TTCTACAGGCAATCACCTGA+TGG + chr6_3:97850952-97850971 Msa0967750:CDS 45.0%
! GCAGTGTTGGAGACTATGAT+CGG + chr6_3:97851187-97851206 Msa0967750:CDS 45.0%
CAAACAGCGCTTAAGATCAG+GGG - chr6_3:97851227-97851246 None:intergenic 45.0%
CCAAACAGCGCTTAAGATCA+GGG - chr6_3:97851228-97851247 None:intergenic 45.0%
ATTACCTGCGTTGTTGAACG+AGG + chr6_3:97851255-97851274 Msa0967750:CDS 45.0%
TTACCTGCGTTGTTGAACGA+GGG + chr6_3:97851256-97851275 Msa0967750:CDS 45.0%
!! AACGAGGGTGCTTTCAAGAT+TGG + chr6_3:97851271-97851290 Msa0967750:CDS 45.0%
! TTCTTGTGCTGCAACGCTTT+TGG - chr6_3:97851334-97851353 None:intergenic 45.0%
GAACGAAATAAACGCCGCCT+GGG + chr6_3:97849888-97849907 Msa0967750:five_prime_UTR 50.0%
GGAGAACCTTGAATACCCAG+CGG + chr6_3:97850039-97850058 Msa0967750:CDS 50.0%
TAACCACCGCTGGGTATTCA+AGG - chr6_3:97850048-97850067 None:intergenic 50.0%
TGGCTTCACTAGCTGCCAAA+TGG + chr6_3:97850185-97850204 Msa0967750:CDS 50.0%
CTCTTCAATCCTCGGATCAG+AGG - chr6_3:97850400-97850419 None:intergenic 50.0%
TGAGTGGTCCTGAGTACTCA+AGG + chr6_3:97850803-97850822 Msa0967750:CDS 50.0%
TGGTCCTGAGTACTCAAGGA+AGG + chr6_3:97850807-97850826 Msa0967750:CDS 50.0%
!! TAGAAAACAGAGGCACCAGG+TGG - chr6_3:97850937-97850956 None:intergenic 50.0%
!! CTGTAGAAAACAGAGGCACC+AGG - chr6_3:97850940-97850959 None:intergenic 50.0%
! CTGGTGCCTCTGTTTTCTAC+AGG + chr6_3:97850938-97850957 Msa0967750:CDS 50.0%
! ATGAGTGCAGCCTCCTTTTC+TGG - chr6_3:97851145-97851164 None:intergenic 50.0%
GGCTGCACTCATAGAGAACA+AGG + chr6_3:97851153-97851172 Msa0967750:CDS 50.0%
GGCAGTTTCATCAGCAGTGT+TGG + chr6_3:97851174-97851193 Msa0967750:CDS 50.0%
! CCCTGATCTTAAGCGCTGTT+TGG + chr6_3:97851225-97851244 Msa0967750:CDS 50.0%
GCCAAACAGCGCTTAAGATC+AGG - chr6_3:97851229-97851248 None:intergenic 50.0%
GCAGCACAAGAATGAGACCA+TGG + chr6_3:97851342-97851361 Msa0967750:three_prime_UTR 50.0%
GAACGAAATAAACGCCGCCT+GGG + chr6_3:97849888-97849907 Msa0967750:five_prime_UTR 50.0%
GGAGAACCTTGAATACCCAG+CGG + chr6_3:97850039-97850058 Msa0967750:CDS 50.0%
TAACCACCGCTGGGTATTCA+AGG - chr6_3:97850048-97850067 None:intergenic 50.0%
TGGCTTCACTAGCTGCCAAA+TGG + chr6_3:97850185-97850204 Msa0967750:CDS 50.0%
CTCTTCAATCCTCGGATCAG+AGG - chr6_3:97850400-97850419 None:intergenic 50.0%
TGAGTGGTCCTGAGTACTCA+AGG + chr6_3:97850803-97850822 Msa0967750:CDS 50.0%
TGGTCCTGAGTACTCAAGGA+AGG + chr6_3:97850807-97850826 Msa0967750:CDS 50.0%
!! TAGAAAACAGAGGCACCAGG+TGG - chr6_3:97850937-97850956 None:intergenic 50.0%
!! CTGTAGAAAACAGAGGCACC+AGG - chr6_3:97850940-97850959 None:intergenic 50.0%
! CTGGTGCCTCTGTTTTCTAC+AGG + chr6_3:97850938-97850957 Msa0967750:CDS 50.0%
! ATGAGTGCAGCCTCCTTTTC+TGG - chr6_3:97851145-97851164 None:intergenic 50.0%
GGCTGCACTCATAGAGAACA+AGG + chr6_3:97851153-97851172 Msa0967750:CDS 50.0%
GGCAGTTTCATCAGCAGTGT+TGG + chr6_3:97851174-97851193 Msa0967750:CDS 50.0%
! CCCTGATCTTAAGCGCTGTT+TGG + chr6_3:97851225-97851244 Msa0967750:CDS 50.0%
GCCAAACAGCGCTTAAGATC+AGG - chr6_3:97851229-97851248 None:intergenic 50.0%
GCAGCACAAGAATGAGACCA+TGG + chr6_3:97851342-97851361 Msa0967750:three_prime_UTR 50.0%
GGAACGAAATAAACGCCGCC+TGG + chr6_3:97849887-97849906 Msa0967750:five_prime_UTR 55.0%
GAACCTTGAATACCCAGCGG+TGG + chr6_3:97850042-97850061 Msa0967750:CDS 55.0%
TCATATTTCCTCGGTGGCGC+TGG + chr6_3:97850088-97850107 Msa0967750:CDS 55.0%
CATATTTCCTCGGTGGCGCT+GGG + chr6_3:97850089-97850108 Msa0967750:CDS 55.0%
ATATTTCCTCGGTGGCGCTG+GGG + chr6_3:97850090-97850109 Msa0967750:CDS 55.0%
GACACATGTCCTCTGATCCG+AGG + chr6_3:97850388-97850407 Msa0967750:intron 55.0%
GGAACGAAATAAACGCCGCC+TGG + chr6_3:97849887-97849906 Msa0967750:five_prime_UTR 55.0%
GAACCTTGAATACCCAGCGG+TGG + chr6_3:97850042-97850061 Msa0967750:CDS 55.0%
TCATATTTCCTCGGTGGCGC+TGG + chr6_3:97850088-97850107 Msa0967750:CDS 55.0%
CATATTTCCTCGGTGGCGCT+GGG + chr6_3:97850089-97850108 Msa0967750:CDS 55.0%
ATATTTCCTCGGTGGCGCTG+GGG + chr6_3:97850090-97850109 Msa0967750:CDS 55.0%
GACACATGTCCTCTGATCCG+AGG + chr6_3:97850388-97850407 Msa0967750:intron 55.0%
AATAGGAAGCGGTCTGGCCC+AGG - chr6_3:97849908-97849927 None:intergenic 60.0%
GTGGTTACCTCTCCGGTCAC+CGG + chr6_3:97850061-97850080 Msa0967750:CDS 60.0%
! TGATTTGCCGGTGACCGGAG+AGG - chr6_3:97850071-97850090 None:intergenic 60.0%
GCACCCTCGTTCAACAACGC+AGG - chr6_3:97851262-97851281 None:intergenic 60.0%
AATAGGAAGCGGTCTGGCCC+AGG - chr6_3:97849908-97849927 None:intergenic 60.0%
GTGGTTACCTCTCCGGTCAC+CGG + chr6_3:97850061-97850080 Msa0967750:CDS 60.0%
! TGATTTGCCGGTGACCGGAG+AGG - chr6_3:97850071-97850090 None:intergenic 60.0%
GCACCCTCGTTCAACAACGC+AGG - chr6_3:97851262-97851281 None:intergenic 60.0%
CCGGAGAGGTAACCACCGCT+GGG - chr6_3:97850057-97850076 None:intergenic 65.0%
CCCAGCGGTGGTTACCTCTC+CGG + chr6_3:97850054-97850073 Msa0967750:CDS 65.0%
ACCGGAGAGGTAACCACCGC+TGG - chr6_3:97850058-97850077 None:intergenic 65.0%
CCGGAGAGGTAACCACCGCT+GGG - chr6_3:97850057-97850076 None:intergenic 65.0%
CCCAGCGGTGGTTACCTCTC+CGG + chr6_3:97850054-97850073 Msa0967750:CDS 65.0%
ACCGGAGAGGTAACCACCGC+TGG - chr6_3:97850058-97850077 None:intergenic 65.0%
AGGAAGCGGTCTGGCCCAGG+CGG - chr6_3:97849905-97849924 None:intergenic 70.0%
AGGAAGCGGTCTGGCCCAGG+CGG - chr6_3:97849905-97849924 None:intergenic 70.0%
CTCTCTCCCCAGCGCCACCG+AGG - chr6_3:97850099-97850118 None:intergenic 75.0%
! CTCGGTGGCGCTGGGGAGAG+AGG + chr6_3:97850097-97850116 Msa0967750:CDS 75.0%
CTCTCTCCCCAGCGCCACCG+AGG - chr6_3:97850099-97850118 None:intergenic 75.0%
! CTCGGTGGCGCTGGGGAGAG+AGG + chr6_3:97850097-97850116 Msa0967750:CDS 75.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 97849827 97851584 97849827 ID=Msa0967750;Name=Msa0967750
chr6_3 mRNA 97849827 97851584 97849827 ID=Msa0967750-mRNA-1;Parent=Msa0967750;Name=Msa0967750-mRNA-1;_AED=0.40;_eAED=0.40;_QI=183|1|1|1|1|1|3|284|222
chr6_3 exon 97849827 97850268 97849827 ID=Msa0967750-mRNA-1:exon:18224;Parent=Msa0967750-mRNA-1
chr6_3 exon 97850761 97850984 97850761 ID=Msa0967750-mRNA-1:exon:18225;Parent=Msa0967750-mRNA-1
chr6_3 exon 97851115 97851584 97851115 ID=Msa0967750-mRNA-1:exon:18226;Parent=Msa0967750-mRNA-1
chr6_3 five_prime_UTR 97849827 97850009 97849827 ID=Msa0967750-mRNA-1:five_prime_utr;Parent=Msa0967750-mRNA-1
chr6_3 CDS 97850010 97850268 97850010 ID=Msa0967750-mRNA-1:cds;Parent=Msa0967750-mRNA-1
chr6_3 CDS 97850761 97850984 97850761 ID=Msa0967750-mRNA-1:cds;Parent=Msa0967750-mRNA-1
chr6_3 CDS 97851115 97851300 97851115 ID=Msa0967750-mRNA-1:cds;Parent=Msa0967750-mRNA-1
chr6_3 three_prime_UTR 97851301 97851584 97851301 ID=Msa0967750-mRNA-1:three_prime_utr;Parent=Msa0967750-mRNA-1
Gene Sequence

>Msa0967750

ATGGCTGCATCAATCACCGCAATCACTGTGGAGAACCTTGAATACCCAGCGGTGGTTACCTCTCCGGTCACCGGCAAATCATATTTCCTCGGTGGCGCTGGGGAGAGAGGATTGACCATTGAAGGAAACTTCATCAAGTTCACTGCCATAGGTGTTTATTTGGAAGATATAGCAGTGGCTTCACTAGCTGCCAAATGGAAGGGTAAATCATCTGAAGAGTTACTTGAGACCCTCGACTTTTACAGAGACATCATCTCAGGTCCCTTTGAAAAGTTAATTAGAGGGTCAAAGATTAGGGAATTGAGTGGTCCTGAGTACTCAAGGAAGGTTATGGAGAACTGTGTGGCACACTTGAAATCAGTTGGAACTTATGGAGATGCAGAAGCTGAAGCTATGCAAAAATTTGCTGAAGCTTTCAAGCCTGTTAATTTTCCACCTGGTGCCTCTGTTTTCTACAGGCAATCACCTGATGGAATATTAGGGCTTAGTTTCTCTCCGGATACAAGTATACCAGAAAAGGAGGCTGCACTCATAGAGAACAAGGCAGTTTCATCAGCAGTGTTGGAGACTATGATCGGCGAACACGCTGTTTCCCCTGATCTTAAGCGCTGTTTGGCTGCAAGATTACCTGCGTTGTTGAACGAGGGTGCTTTCAAGATTGGAAACTGA
Protein sequence

>Msa0967750

MAASITAITVENLEYPAVVTSPVTGKSYFLGGAGERGLTIEGNFIKFTAIGVYLEDIAVASLAAKWKGKSSEELLETLDFYRDIISGPFEKLIRGSKIRELSGPEYSRKVMENCVAHLKSVGTYGDAEAEAMQKFAEAFKPVNFPPGASVFYRQSPDGILGLSFSPDTSIPEKEAALIENKAVSSAVLETMIGEHAVSPDLKRCLAARLPALLNEGAFKIGN*