AlfalfaGEDB | Msa0139530

M. sativa cultivar zhongmu-4 / Msa0139530

KEGG: K01859
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0008150 GO:0008152 GO:0009058 GO:0009628 GO:0009812 GO:0009813 GO:0012505 GO:0016853 GO:0016872 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045430 GO:0050896 GO:0071704 GO:0080167 GO:1901576
PFAM: PF02431.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0139530	sp\|P28012\|CFI1_MEDSA	100.000	222	0	0	1	222	1	222	6.02e-162	449

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0139530	U5TRY3	100.000	222	0	0	1	222	1	222	2.30e-159	449

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0046170	Msa0139530	0.990592	2.266864e-183	-8.615850e-47
Msa0090380	Msa0139530	0.810855	9.364142e-51	-8.615850e-47
Msa0139530	Msa0242720	0.810857	9.357793e-51	-8.615850e-47
Msa0139530	Msa0320170	0.809064	2.275551e-50	-8.615850e-47
Msa0139530	Msa0590390	0.803177	3.947192e-49	-8.615850e-47
Msa0139530	Msa0615200	0.818838	1.590602e-52	-8.615850e-47
Msa0139530	Msa0615210	0.819032	1.437052e-52	-8.615850e-47
Msa0139530	Msa0652590	0.815006	1.153103e-51	-8.615850e-47
Msa0139530	Msa0754480	0.807602	4.666308e-50	-8.615850e-47
Msa0139530	Msa0967750	0.973447	1.917393e-136	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0139530	MtrunA17_Chr1g0213011	98.198	222	4	0	1	222	1	222	1.01e-161	445
Msa0139530	MtrunA17_Chr1g0213021	62.896	221	82	0	2	222	5	225	7.70e-98	284
Msa0139530	MtrunA17_Chr1g0213031	61.187	219	85	0	2	220	5	223	8.08e-93	271
Msa0139530	MtrunA17_Chr1g0213041	54.884	215	95	1	4	216	5	219	1.75e-80	240
Msa0139530	MtrunA17_Chr1g0213051	52.804	214	99	1	4	215	5	218	7.94e-78	233
Msa0139530	MtrunA17_Chr7g0224381	50.000	162	81	0	4	165	5	166	4.81e-56	176
Msa0139530	MtrunA17_Chr3g0128761	32.086	187	116	5	29	209	15	196	1.83e-11	61.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0139530	AT3G55120.1	49.048	210	107	0	4	213	15	224	3.16e-67	207
Msa0139530	AT5G66230.2	42.453	212	114	1	4	215	5	208	1.27e-53	180
Msa0139530	AT5G66220.1	42.767	159	91	0	57	215	1	159	5.51e-44	145
Msa0139530	AT5G05270.1	28.708	209	142	4	8	214	6	209	6.90e-13	65.5
Msa0139530	AT5G05270.2	28.708	209	142	4	8	214	6	209	6.90e-13	65.5

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Find 80 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCAAAATAGGTGAAT+TGG	0.349824	1_3:-73196806	Msa0139530:five_prime_UTR
AAATATGATTTGCCGGTGAC+CGG	0.472992	1_3:+73196577	None:intergenic
AACGAGGGTGCTTTCAAGAT+TGG	0.337469	1_3:-73195382	Msa0139530:CDS
AAGGTTCTCCACAGTGATTG+CGG	0.648293	1_3:+73196624	None:intergenic
AATAATTAAGAACAAGAATA+TGG	0.334406	1_3:-73196659	Msa0139530:exon
AATAGGAAGCGGTCTGGCCC+AGG	0.492465	1_3:+73196744	None:intergenic
AATCACCTGATGGAATATTA+GGG	0.365814	1_3:-73195685	Msa0139530:CDS
AATTAGAGGGTCAAAGATTA+GGG	0.310626	1_3:-73195870	Msa0139530:CDS
ACCGAGGAAATATGATTTGC+CGG	0.416952	1_3:+73196570	None:intergenic
ACCGGAGAGGTAACCACCGC+TGG	0.477165	1_3:+73196595	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCTTCCAAATAAACACCTA+TGG	+	chr1_3:73195421-73195440	None:intergenic	30.0%
TTATTTGGAAGATATAGCAG+TGG	-	chr1_3:73195428-73195447	Msa0139530:CDS	30.0%
GTGAGTAAAAACTAGTATGA+AGG	+	chr1_3:73195693-73195712	None:intergenic	30.0%
TAATTAGAGGGTCAAAGATT+AGG	-	chr1_3:73196042-73196061	Msa0139530:intron	30.0%
AATTAGAGGGTCAAAGATTA+GGG	-	chr1_3:73196043-73196062	Msa0139530:intron	30.0%
TTGAAATCAGTTGGAACTTA+TGG	-	chr1_3:73196119-73196138	Msa0139530:intron	30.0%
AATCACCTGATGGAATATTA+GGG	-	chr1_3:73196228-73196247	Msa0139530:intron	30.0%
TGTAGACTTAAAAAAGTCCA+TGG	+	chr1_3:73196622-73196641	None:intergenic	30.0%
ATCTTCCAAATAAACACCTA+TGG	+	chr1_3:73195421-73195440	None:intergenic	30.0%
TTATTTGGAAGATATAGCAG+TGG	-	chr1_3:73195428-73195447	Msa0139530:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	73195091	73196844	73195091	ID=Msa0139530;Name=Msa0139530
chr1_3	mRNA	73195091	73196844	73195091	ID=Msa0139530-mRNA-1;Parent=Msa0139530;Name=Msa0139530-mRNA-1;_AED=0.41;_eAED=0.41;_QI=182\|1\|1\|1\|1\|1\|3\|284\|222
chr1_3	exon	73195091	73195560	73195091	ID=Msa0139530-mRNA-1:exon:25564;Parent=Msa0139530-mRNA-1
chr1_3	exon	73195685	73195908	73195685	ID=Msa0139530-mRNA-1:exon:25563;Parent=Msa0139530-mRNA-1
chr1_3	exon	73196404	73196844	73196404	ID=Msa0139530-mRNA-1:exon:25562;Parent=Msa0139530-mRNA-1
chr1_3	five_prime_UTR	73196663	73196844	73196663	ID=Msa0139530-mRNA-1:five_prime_utr;Parent=Msa0139530-mRNA-1
chr1_3	CDS	73196404	73196662	73196404	ID=Msa0139530-mRNA-1:cds;Parent=Msa0139530-mRNA-1
chr1_3	CDS	73195685	73195908	73195685	ID=Msa0139530-mRNA-1:cds;Parent=Msa0139530-mRNA-1
chr1_3	CDS	73195375	73195560	73195375	ID=Msa0139530-mRNA-1:cds;Parent=Msa0139530-mRNA-1
chr1_3	three_prime_UTR	73195091	73195374	73195091	ID=Msa0139530-mRNA-1:three_prime_utr;Parent=Msa0139530-mRNA-1

Gene Sequence

>Msa0139530

ATGGCTGCATCAATCACCGCAATCACTGTGGAGAACCTTGAATACCCAGCGGTGGTTACCTCTCCGGTCACCGGCAAATCATATTTCCTCGGTGGCGCTGGGGAGAGAGGATTGACCATTGAAGGAAACTTCATCAAGTTCACTGCCATAGGTGTTTATTTGGAAGATATAGCAGTGGCTTCACTAGCTGCCAAATGGAAGGGTAAATCATCTGAAGAGTTACTTGAGACCCTCGACTTTTACAGAGACATCATCTCAGGTCCCTTTGAAAAGTTAATTAGAGGGTCAAAGATTAGGGAATTGAGTGGTCCTGAGTACTCAAGGAAGGTTATGGAGAACTGTGTGGCACACTTGAAATCAGTTGGAACTTATGGAGATGCAGAAGCTGAAGCTATGCAAAAATTTGCTGAAGCTTTCAAGCCTGTTAATTTTCCACCTGGTGCCTCTGTTTTCTACAGGCAATCACCTGATGGAATATTAGGGCTTAGTTTCTCTCCGGATACAAGTATACCAGAAAAGGAGGCTGCACTCATAGAGAACAAGGCAGTTTCATCAGCAGTGTTGGAGACTATGATCGGCGAACACGCTGTTTCCCCTGATCTTAAGCGCTGTTTGGCTGCAAGATTACCTGCGTTGTTGAACGAGGGTGCTTTCAAGATTGGAAACTGA

Protein sequence

>Msa0139530

MAASITAITVENLEYPAVVTSPVTGKSYFLGGAGERGLTIEGNFIKFTAIGVYLEDIAVASLAAKWKGKSSEELLETLDFYRDIISGPFEKLIRGSKIRELSGPEYSRKVMENCVAHLKSVGTYGDAEAEAMQKFAEAFKPVNFPPGASVFYRQSPDGILGLSFSPDTSIPEKEAALIENKAVSSAVLETMIGEHAVSPDLKRCLAARLPALLNEGAFKIGN*