AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0139530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0139530 MtrunA17_Chr1g0213011 98.198 222 4 0 1 222 1 222 1.01e-161 445
Msa0139530 MtrunA17_Chr1g0213021 62.896 221 82 0 2 222 5 225 7.70e-98 284
Msa0139530 MtrunA17_Chr1g0213031 61.187 219 85 0 2 220 5 223 8.08e-93 271
Msa0139530 MtrunA17_Chr1g0213041 54.884 215 95 1 4 216 5 219 1.75e-80 240
Msa0139530 MtrunA17_Chr1g0213051 52.804 214 99 1 4 215 5 218 7.94e-78 233
Msa0139530 MtrunA17_Chr7g0224381 50.000 162 81 0 4 165 5 166 4.81e-56 176
Msa0139530 MtrunA17_Chr3g0128761 32.086 187 116 5 29 209 15 196 1.83e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0139530 AT3G55120.1 49.048 210 107 0 4 213 15 224 3.16e-67 207
Msa0139530 AT5G66230.2 42.453 212 114 1 4 215 5 208 1.27e-53 180
Msa0139530 AT5G66220.1 42.767 159 91 0 57 215 1 159 5.51e-44 145
Msa0139530 AT5G05270.1 28.708 209 142 4 8 214 6 209 6.90e-13 65.5
Msa0139530 AT5G05270.2 28.708 209 142 4 8 214 6 209 6.90e-13 65.5

Find 80 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGACCAATTCACCTATTT+TGG 0.093565 1_3:+73196801 None:intergenic
AGCTATGATGTTTCAGATTT+TGG 0.159725 1_3:+73196689 None:intergenic
CAATCACCTGATGGAATATT+AGG 0.209322 1_3:-73195686 Msa0139530:CDS
CACTGCCATAGGTGTTTATT+TGG 0.211608 1_3:-73196500 Msa0139530:CDS
TTGAAATCAGTTGGAACTTA+TGG 0.230001 1_3:-73195794 Msa0139530:CDS
TTCTTGTGCTGCAACGCTTT+TGG 0.248420 1_3:+73195322 None:intergenic
AATTAGAGGGTCAAAGATTA+GGG 0.310626 1_3:-73195870 Msa0139530:CDS
CTTGATGAAGTTTCCTTCAA+TGG 0.330932 1_3:+73196525 None:intergenic
CCCTGATCTTAAGCGCTGTT+TGG 0.332738 1_3:-73195428 Msa0139530:CDS
AATAATTAAGAACAAGAATA+TGG 0.334406 1_3:-73196659 Msa0139530:exon
AACGAGGGTGCTTTCAAGAT+TGG 0.337469 1_3:-73195382 Msa0139530:CDS
TAATTAGAGGGTCAAAGATT+AGG 0.343487 1_3:-73195871 Msa0139530:CDS
AAAATCCAAAATAGGTGAAT+TGG 0.349824 1_3:-73196806 Msa0139530:five_prime_UTR
TCATATTTCCTCGGTGGCGC+TGG 0.355424 1_3:-73196562 Msa0139530:CDS
CCCTTTGAAAAGTTAATTAG+AGG 0.361486 1_3:-73195884 Msa0139530:CDS
AATCACCTGATGGAATATTA+GGG 0.365814 1_3:-73195685 Msa0139530:CDS
TCAAATAAAATACATGCAAC+TGG 0.398245 1_3:-73196783 Msa0139530:five_prime_UTR
GCCAAACAGCGCTTAAGATC+AGG 0.410390 1_3:+73195427 None:intergenic
GCACCAGGTGGAAAATTAAC+AGG 0.410631 1_3:+73195725 None:intergenic
ACCGAGGAAATATGATTTGC+CGG 0.416952 1_3:+73196570 None:intergenic
AGATGATTTACCCTTCCATT+TGG 0.439741 1_3:+73196450 None:intergenic
GGCAGTTTCATCAGCAGTGT+TGG 0.459798 1_3:-73195479 Msa0139530:CDS
CTCGGTGGCGCTGGGGAGAG+AGG 0.461142 1_3:-73196553 Msa0139530:CDS
CATATTTCCTCGGTGGCGCT+GGG 0.461249 1_3:-73196561 Msa0139530:CDS
ACTTGCGTTTAGTGAAGAAC+GGG 0.463930 1_3:+73196717 None:intergenic
GGAACGAAATAAACGCCGCC+TGG 0.467910 1_3:-73196762 Msa0139530:five_prime_UTR
AAATATGATTTGCCGGTGAC+CGG 0.472992 1_3:+73196577 None:intergenic
ACCGGAGAGGTAACCACCGC+TGG 0.477165 1_3:+73196595 None:intergenic
TGAGTACTCAAGGAAGGTTA+TGG 0.487878 1_3:-73195834 Msa0139530:CDS
TAACCACCGCTGGGTATTCA+AGG 0.488480 1_3:+73196605 None:intergenic
GGATACAAGTATACCAGAAA+AGG 0.490457 1_3:-73195524 Msa0139530:CDS
AATAGGAAGCGGTCTGGCCC+AGG 0.492465 1_3:+73196744 None:intergenic
CCCAGCGGTGGTTACCTCTC+CGG 0.492838 1_3:-73196596 Msa0139530:CDS
TATTATTGATACTAATAAGA+AGG 0.499753 1_3:+73195245 None:intergenic
TGGCTTCACTAGCTGCCAAA+TGG 0.503292 1_3:-73196465 Msa0139530:CDS
GAGAGAGGATTGACCATTGA+AGG 0.509487 1_3:-73196538 Msa0139530:CDS
CTAACCCCTAATATTCCATC+AGG 0.511312 1_3:+73195680 None:intergenic
ATCTTCCAAATAAACACCTA+TGG 0.512141 1_3:+73196495 None:intergenic
GTGGTTACCTCTCCGGTCAC+CGG 0.524005 1_3:-73196589 Msa0139530:CDS
TTCACTAGCTGCCAAATGGA+AGG 0.526249 1_3:-73196461 Msa0139530:CDS
CCAAACAGCGCTTAAGATCA+GGG 0.532079 1_3:+73195428 None:intergenic
TATGCAGCTTAGTTTCTCTC+CGG 0.547394 1_3:-73195545 Msa0139530:intron
GACTTGCGTTTAGTGAAGAA+CGG 0.548731 1_3:+73196716 None:intergenic
TTCTACAGGCAATCACCTGA+TGG 0.554083 1_3:-73195695 Msa0139530:CDS
GAACGAAATAAACGCCGCCT+GGG 0.554703 1_3:-73196761 Msa0139530:five_prime_UTR
GGCTGCACTCATAGAGAACA+AGG 0.569164 1_3:-73195500 Msa0139530:CDS
ACCGGCAAATCATATTTCCT+CGG 0.576452 1_3:-73196571 Msa0139530:CDS
TGAGTGGTCCTGAGTACTCA+AGG 0.577740 1_3:-73195844 Msa0139530:CDS
CTCTCTCCCCAGCGCCACCG+AGG 0.582911 1_3:+73196554 None:intergenic
CTGTAGAAAACAGAGGCACC+AGG 0.591901 1_3:+73195710 None:intergenic
CCTTTGAAAAGTTAATTAGA+GGG 0.595496 1_3:-73195883 Msa0139530:CDS
ATTACCTGCGTTGTTGAACG+AGG 0.600190 1_3:-73195398 Msa0139530:CDS
TGATGTCTCTGTAAAAGTCG+AGG 0.602205 1_3:+73196410 None:intergenic
ATAACCTTCCTTGAGTACTC+AGG 0.607676 1_3:+73195836 None:intergenic
TTCATCAAGTTCACTGCCAT+AGG 0.610295 1_3:-73196511 Msa0139530:CDS
GCAGTGTTGGAGACTATGAT+CGG 0.622152 1_3:-73195466 Msa0139530:CDS
AGGAAGCGGTCTGGCCCAGG+CGG 0.623344 1_3:+73196747 None:intergenic
TCACTAGCTGCCAAATGGAA+GGG 0.624312 1_3:-73196460 Msa0139530:CDS
GTGGCACACTTGAAATCAGT+TGG 0.625898 1_3:-73195803 Msa0139530:CDS
GATGTCTCTGTAAAAGTCGA+GGG 0.629213 1_3:+73196411 None:intergenic
CCGGAGAGGTAACCACCGCT+GGG 0.632340 1_3:+73196596 None:intergenic
TACAAGTATACCAGAAAAGG+AGG 0.632960 1_3:-73195521 Msa0139530:CDS
TGGTCCTGAGTACTCAAGGA+AGG 0.639707 1_3:-73195840 Msa0139530:CDS
GGCAAATCATATTTCCTCGG+TGG 0.648065 1_3:-73196568 Msa0139530:CDS
AAGGTTCTCCACAGTGATTG+CGG 0.648293 1_3:+73196624 None:intergenic
ATATTTCCTCGGTGGCGCTG+GGG 0.654176 1_3:-73196560 Msa0139530:CDS
GCACCCTCGTTCAACAACGC+AGG 0.670342 1_3:+73195394 None:intergenic
TGATTTGCCGGTGACCGGAG+AGG 0.671162 1_3:+73196582 None:intergenic
TTATTTGGAAGATATAGCAG+TGG 0.671898 1_3:-73196485 Msa0139530:CDS
GAACCTTGAATACCCAGCGG+TGG 0.677026 1_3:-73196608 Msa0139530:CDS
CAAACAGCGCTTAAGATCAG+GGG 0.682290 1_3:+73195429 None:intergenic
TTACCTGCGTTGTTGAACGA+GGG 0.686961 1_3:-73195397 Msa0139530:CDS
ATCACCTGATGGAATATTAG+GGG 0.689762 1_3:-73195684 Msa0139530:intron
TCAAAGATTAGGGAATTGAG+TGG 0.698363 1_3:-73195860 Msa0139530:CDS
TAGAAAACAGAGGCACCAGG+TGG 0.700225 1_3:+73195713 None:intergenic
GCAGCACAAGAATGAGACCA+TGG 0.722638 1_3:-73195311 Msa0139530:three_prime_UTR
ATCAATCACCGCAATCACTG+TGG 0.724881 1_3:-73196632 Msa0139530:CDS
GAAGGTTATGGAGAACTGTG+TGG 0.734369 1_3:-73195822 Msa0139530:CDS
TGATTGCCTGTAGAAAACAG+AGG 0.767471 1_3:+73195703 None:intergenic
GGAGAACCTTGAATACCCAG+CGG 0.782943 1_3:-73196611 Msa0139530:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTAAGAACAAGAATA+TGG - chr1_3:73195254-73195273 Msa0139530:three_prime_UTR 15.0%
!! AAATCATTTAATTAATTGCA+AGG + chr1_3:73195569-73195588 None:intergenic 15.0%
!! GTAATACTATAGTATTTAAT+TGG + chr1_3:73195888-73195907 None:intergenic 15.0%
!!! TGATAAATTGATAATTTTGT+AGG - chr1_3:73196005-73196024 Msa0139530:intron 15.0%
!!! TTTTAATATACTAGTATTAG+TGG + chr1_3:73196345-73196364 None:intergenic 15.0%
!! TATTATTGATACTAATAAGA+AGG + chr1_3:73196671-73196690 None:intergenic 15.0%
!! AATAATTAAGAACAAGAATA+TGG - chr1_3:73195254-73195273 Msa0139530:three_prime_UTR 15.0%
!! AAATCATTTAATTAATTGCA+AGG + chr1_3:73195569-73195588 None:intergenic 15.0%
!! GTAATACTATAGTATTTAAT+TGG + chr1_3:73195888-73195907 None:intergenic 15.0%
!!! TGATAAATTGATAATTTTGT+AGG - chr1_3:73196005-73196024 Msa0139530:intron 15.0%
!!! TTTTAATATACTAGTATTAG+TGG + chr1_3:73196345-73196364 None:intergenic 15.0%
!! TATTATTGATACTAATAAGA+AGG + chr1_3:73196671-73196690 None:intergenic 15.0%
!! AGAAAAGAAAAATCCAAAAT+AGG - chr1_3:73195099-73195118 Msa0139530:three_prime_UTR 20.0%
!! GTTAGACTTTAATTAATTCT+TGG + chr1_3:73195797-73195816 None:intergenic 20.0%
!! AATTAAAGTCTAACATACAA+CGG - chr1_3:73195803-73195822 Msa0139530:CDS 20.0%
!! AGTTAAGAAACAGAATTAAT+TGG + chr1_3:73196267-73196286 None:intergenic 20.0%
!!! TTTCTTAACTATTTTAGTTG+AGG - chr1_3:73196277-73196296 Msa0139530:intron 20.0%
!! AGAAAAGAAAAATCCAAAAT+AGG - chr1_3:73195099-73195118 Msa0139530:three_prime_UTR 20.0%
!! GTTAGACTTTAATTAATTCT+TGG + chr1_3:73195797-73195816 None:intergenic 20.0%
!! AATTAAAGTCTAACATACAA+CGG - chr1_3:73195803-73195822 Msa0139530:CDS 20.0%
!! AGTTAAGAAACAGAATTAAT+TGG + chr1_3:73196267-73196286 None:intergenic 20.0%
!!! TTTCTTAACTATTTTAGTTG+AGG - chr1_3:73196277-73196296 Msa0139530:intron 20.0%
! AAAATCCAAAATAGGTGAAT+TGG - chr1_3:73195107-73195126 Msa0139530:three_prime_UTR 25.0%
! TCAAATAAAATACATGCAAC+TGG - chr1_3:73195130-73195149 Msa0139530:three_prime_UTR 25.0%
!!! CATTTTTTAACATATGAAGC+TGG - chr1_3:73195624-73195643 Msa0139530:intron 25.0%
! TCAATAAGACTCATATGATT+TGG + chr1_3:73195762-73195781 None:intergenic 25.0%
! TTTAATTGGATGAATTCAAG+TGG + chr1_3:73195874-73195893 None:intergenic 25.0%
!! CCTCTAATTAACTTTTCAAA+GGG + chr1_3:73196032-73196051 None:intergenic 25.0%
!! CCTTTGAAAAGTTAATTAGA+GGG - chr1_3:73196030-73196049 Msa0139530:intron 25.0%
! ATATACTAGTATTAGTGGAA+AGG + chr1_3:73196340-73196359 None:intergenic 25.0%
! AAAATCCAAAATAGGTGAAT+TGG - chr1_3:73195107-73195126 Msa0139530:three_prime_UTR 25.0%
! TCAAATAAAATACATGCAAC+TGG - chr1_3:73195130-73195149 Msa0139530:three_prime_UTR 25.0%
!!! CATTTTTTAACATATGAAGC+TGG - chr1_3:73195624-73195643 Msa0139530:intron 25.0%
! TCAATAAGACTCATATGATT+TGG + chr1_3:73195762-73195781 None:intergenic 25.0%
! TTTAATTGGATGAATTCAAG+TGG + chr1_3:73195874-73195893 None:intergenic 25.0%
!! CCTCTAATTAACTTTTCAAA+GGG + chr1_3:73196032-73196051 None:intergenic 25.0%
!! CCTTTGAAAAGTTAATTAGA+GGG - chr1_3:73196030-73196049 Msa0139530:intron 25.0%
! ATATACTAGTATTAGTGGAA+AGG + chr1_3:73196340-73196359 None:intergenic 25.0%
! TTTGACCAATTCACCTATTT+TGG + chr1_3:73195115-73195134 None:intergenic 30.0%
! AGCTATGATGTTTCAGATTT+TGG + chr1_3:73195227-73195246 None:intergenic 30.0%
ATCTTCCAAATAAACACCTA+TGG + chr1_3:73195421-73195440 None:intergenic 30.0%
TTATTTGGAAGATATAGCAG+TGG - chr1_3:73195428-73195447 Msa0139530:CDS 30.0%
GTGAGTAAAAACTAGTATGA+AGG + chr1_3:73195693-73195712 None:intergenic 30.0%
!! CTCATATGATTTGGTTGATT+AGG + chr1_3:73195753-73195772 None:intergenic 30.0%
!! AGTCTTATTGATGTAGAGTT+CGG - chr1_3:73195771-73195790 Msa0139530:CDS 30.0%
! CCCTTTGAAAAGTTAATTAG+AGG - chr1_3:73196029-73196048 Msa0139530:intron 30.0%
! CCCTCTAATTAACTTTTCAA+AGG + chr1_3:73196033-73196052 None:intergenic 30.0%
TAATTAGAGGGTCAAAGATT+AGG - chr1_3:73196042-73196061 Msa0139530:intron 30.0%
AATTAGAGGGTCAAAGATTA+GGG - chr1_3:73196043-73196062 Msa0139530:intron 30.0%
TTGAAATCAGTTGGAACTTA+TGG - chr1_3:73196119-73196138 Msa0139530:intron 30.0%
AATCACCTGATGGAATATTA+GGG - chr1_3:73196228-73196247 Msa0139530:intron 30.0%
!! TTTTCTGGTATACTTGTATC+CGG + chr1_3:73196390-73196409 None:intergenic 30.0%
TGTAGACTTAAAAAAGTCCA+TGG + chr1_3:73196622-73196641 None:intergenic 30.0%
! TTTGACCAATTCACCTATTT+TGG + chr1_3:73195115-73195134 None:intergenic 30.0%
! AGCTATGATGTTTCAGATTT+TGG + chr1_3:73195227-73195246 None:intergenic 30.0%
ATCTTCCAAATAAACACCTA+TGG + chr1_3:73195421-73195440 None:intergenic 30.0%
TTATTTGGAAGATATAGCAG+TGG - chr1_3:73195428-73195447 Msa0139530:CDS 30.0%
GTGAGTAAAAACTAGTATGA+AGG + chr1_3:73195693-73195712 None:intergenic 30.0%
!! CTCATATGATTTGGTTGATT+AGG + chr1_3:73195753-73195772 None:intergenic 30.0%
!! AGTCTTATTGATGTAGAGTT+CGG - chr1_3:73195771-73195790 Msa0139530:CDS 30.0%
! CCCTTTGAAAAGTTAATTAG+AGG - chr1_3:73196029-73196048 Msa0139530:intron 30.0%
! CCCTCTAATTAACTTTTCAA+AGG + chr1_3:73196033-73196052 None:intergenic 30.0%
TAATTAGAGGGTCAAAGATT+AGG - chr1_3:73196042-73196061 Msa0139530:intron 30.0%
AATTAGAGGGTCAAAGATTA+GGG - chr1_3:73196043-73196062 Msa0139530:intron 30.0%
TTGAAATCAGTTGGAACTTA+TGG - chr1_3:73196119-73196138 Msa0139530:intron 30.0%
AATCACCTGATGGAATATTA+GGG - chr1_3:73196228-73196247 Msa0139530:intron 30.0%
!! TTTTCTGGTATACTTGTATC+CGG + chr1_3:73196390-73196409 None:intergenic 30.0%
TGTAGACTTAAAAAAGTCCA+TGG + chr1_3:73196622-73196641 None:intergenic 30.0%
!! AGTGAAGAACGGGTTTTAAT+AGG + chr1_3:73195189-73195208 None:intergenic 35.0%
CTTGATGAAGTTTCCTTCAA+TGG + chr1_3:73195391-73195410 None:intergenic 35.0%
AGATGATTTACCCTTCCATT+TGG + chr1_3:73195466-73195485 None:intergenic 35.0%
! TTTTACAGAGACATCATCTC+AGG - chr1_3:73195510-73195529 Msa0139530:CDS 35.0%
TCAAAGATTAGGGAATTGAG+TGG - chr1_3:73196053-73196072 Msa0139530:intron 35.0%
CAATCACCTGATGGAATATT+AGG - chr1_3:73196227-73196246 Msa0139530:intron 35.0%
ATCACCTGATGGAATATTAG+GGG - chr1_3:73196229-73196248 Msa0139530:intron 35.0%
GGATACAAGTATACCAGAAA+AGG - chr1_3:73196389-73196408 Msa0139530:intron 35.0%
TACAAGTATACCAGAAAAGG+AGG - chr1_3:73196392-73196411 Msa0139530:intron 35.0%
!! AGTGAAGAACGGGTTTTAAT+AGG + chr1_3:73195189-73195208 None:intergenic 35.0%
CTTGATGAAGTTTCCTTCAA+TGG + chr1_3:73195391-73195410 None:intergenic 35.0%
AGATGATTTACCCTTCCATT+TGG + chr1_3:73195466-73195485 None:intergenic 35.0%
! TTTTACAGAGACATCATCTC+AGG - chr1_3:73195510-73195529 Msa0139530:CDS 35.0%
TCAAAGATTAGGGAATTGAG+TGG - chr1_3:73196053-73196072 Msa0139530:intron 35.0%
CAATCACCTGATGGAATATT+AGG - chr1_3:73196227-73196246 Msa0139530:intron 35.0%
ATCACCTGATGGAATATTAG+GGG - chr1_3:73196229-73196248 Msa0139530:intron 35.0%
GGATACAAGTATACCAGAAA+AGG - chr1_3:73196389-73196408 Msa0139530:intron 35.0%
TACAAGTATACCAGAAAAGG+AGG - chr1_3:73196392-73196411 Msa0139530:intron 35.0%
!! GAACGGGTTTTAATAGGAAG+CGG + chr1_3:73195183-73195202 None:intergenic 40.0%
ACTTGCGTTTAGTGAAGAAC+GGG + chr1_3:73195199-73195218 None:intergenic 40.0%
GACTTGCGTTTAGTGAAGAA+CGG + chr1_3:73195200-73195219 None:intergenic 40.0%
! AAATATGATTTGCCGGTGAC+CGG + chr1_3:73195339-73195358 None:intergenic 40.0%
ACCGGCAAATCATATTTCCT+CGG - chr1_3:73195342-73195361 Msa0139530:three_prime_UTR 40.0%
! ACCGAGGAAATATGATTTGC+CGG + chr1_3:73195346-73195365 None:intergenic 40.0%
TTCATCAAGTTCACTGCCAT+AGG - chr1_3:73195402-73195421 Msa0139530:CDS 40.0%
CACTGCCATAGGTGTTTATT+TGG - chr1_3:73195413-73195432 Msa0139530:CDS 40.0%
GATGTCTCTGTAAAAGTCGA+GGG + chr1_3:73195505-73195524 None:intergenic 40.0%
TGATGTCTCTGTAAAAGTCG+AGG + chr1_3:73195506-73195525 None:intergenic 40.0%
ATAACCTTCCTTGAGTACTC+AGG + chr1_3:73196080-73196099 None:intergenic 40.0%
TGAGTACTCAAGGAAGGTTA+TGG - chr1_3:73196079-73196098 Msa0139530:intron 40.0%
! AAGCCTGTTAATTTTCCACC+TGG - chr1_3:73196185-73196204 Msa0139530:intron 40.0%
TGATTGCCTGTAGAAAACAG+AGG + chr1_3:73196213-73196232 None:intergenic 40.0%
CTAACCCCTAATATTCCATC+AGG + chr1_3:73196236-73196255 None:intergenic 40.0%
TATGCAGCTTAGTTTCTCTC+CGG - chr1_3:73196368-73196387 Msa0139530:intron 40.0%
TGAGTAGAGTTGCTTAACTG+AGG - chr1_3:73196824-73196843 Msa0139530:five_prime_UTR 40.0%
!! GAACGGGTTTTAATAGGAAG+CGG + chr1_3:73195183-73195202 None:intergenic 40.0%
ACTTGCGTTTAGTGAAGAAC+GGG + chr1_3:73195199-73195218 None:intergenic 40.0%
GACTTGCGTTTAGTGAAGAA+CGG + chr1_3:73195200-73195219 None:intergenic 40.0%
! AAATATGATTTGCCGGTGAC+CGG + chr1_3:73195339-73195358 None:intergenic 40.0%
ACCGGCAAATCATATTTCCT+CGG - chr1_3:73195342-73195361 Msa0139530:three_prime_UTR 40.0%
! ACCGAGGAAATATGATTTGC+CGG + chr1_3:73195346-73195365 None:intergenic 40.0%
TTCATCAAGTTCACTGCCAT+AGG - chr1_3:73195402-73195421 Msa0139530:CDS 40.0%
CACTGCCATAGGTGTTTATT+TGG - chr1_3:73195413-73195432 Msa0139530:CDS 40.0%
GATGTCTCTGTAAAAGTCGA+GGG + chr1_3:73195505-73195524 None:intergenic 40.0%
TGATGTCTCTGTAAAAGTCG+AGG + chr1_3:73195506-73195525 None:intergenic 40.0%
ATAACCTTCCTTGAGTACTC+AGG + chr1_3:73196080-73196099 None:intergenic 40.0%
TGAGTACTCAAGGAAGGTTA+TGG - chr1_3:73196079-73196098 Msa0139530:intron 40.0%
! AAGCCTGTTAATTTTCCACC+TGG - chr1_3:73196185-73196204 Msa0139530:intron 40.0%
TGATTGCCTGTAGAAAACAG+AGG + chr1_3:73196213-73196232 None:intergenic 40.0%
CTAACCCCTAATATTCCATC+AGG + chr1_3:73196236-73196255 None:intergenic 40.0%
TATGCAGCTTAGTTTCTCTC+CGG - chr1_3:73196368-73196387 Msa0139530:intron 40.0%
!! GGTTTTAATAGGAAGCGGTC+TGG + chr1_3:73195178-73195197 None:intergenic 45.0%
ATCAATCACCGCAATCACTG+TGG - chr1_3:73195281-73195300 Msa0139530:three_prime_UTR 45.0%
AAGGTTCTCCACAGTGATTG+CGG + chr1_3:73195292-73195311 None:intergenic 45.0%
GGCAAATCATATTTCCTCGG+TGG - chr1_3:73195345-73195364 Msa0139530:three_prime_UTR 45.0%
GAGAGAGGATTGACCATTGA+AGG - chr1_3:73195375-73195394 Msa0139530:CDS 45.0%
TTCACTAGCTGCCAAATGGA+AGG - chr1_3:73195452-73195471 Msa0139530:CDS 45.0%
TCACTAGCTGCCAAATGGAA+GGG - chr1_3:73195453-73195472 Msa0139530:CDS 45.0%
GAAGGTTATGGAGAACTGTG+TGG - chr1_3:73196091-73196110 Msa0139530:intron 45.0%
GTGGCACACTTGAAATCAGT+TGG - chr1_3:73196110-73196129 Msa0139530:intron 45.0%
GCACCAGGTGGAAAATTAAC+AGG + chr1_3:73196191-73196210 None:intergenic 45.0%
TTCTACAGGCAATCACCTGA+TGG - chr1_3:73196218-73196237 Msa0139530:intron 45.0%
! GCAGTGTTGGAGACTATGAT+CGG - chr1_3:73196447-73196466 Msa0139530:CDS 45.0%
CAAACAGCGCTTAAGATCAG+GGG + chr1_3:73196487-73196506 None:intergenic 45.0%
CCAAACAGCGCTTAAGATCA+GGG + chr1_3:73196488-73196507 None:intergenic 45.0%
ATTACCTGCGTTGTTGAACG+AGG - chr1_3:73196515-73196534 Msa0139530:CDS 45.0%
TTACCTGCGTTGTTGAACGA+GGG - chr1_3:73196516-73196535 Msa0139530:CDS 45.0%
!! AACGAGGGTGCTTTCAAGAT+TGG - chr1_3:73196531-73196550 Msa0139530:CDS 45.0%
! TTCTTGTGCTGCAACGCTTT+TGG + chr1_3:73196594-73196613 None:intergenic 45.0%
!! GGTTTTAATAGGAAGCGGTC+TGG + chr1_3:73195178-73195197 None:intergenic 45.0%
ATCAATCACCGCAATCACTG+TGG - chr1_3:73195281-73195300 Msa0139530:three_prime_UTR 45.0%
AAGGTTCTCCACAGTGATTG+CGG + chr1_3:73195292-73195311 None:intergenic 45.0%
GGCAAATCATATTTCCTCGG+TGG - chr1_3:73195345-73195364 Msa0139530:three_prime_UTR 45.0%
GAGAGAGGATTGACCATTGA+AGG - chr1_3:73195375-73195394 Msa0139530:CDS 45.0%
TTCACTAGCTGCCAAATGGA+AGG - chr1_3:73195452-73195471 Msa0139530:CDS 45.0%
TCACTAGCTGCCAAATGGAA+GGG - chr1_3:73195453-73195472 Msa0139530:CDS 45.0%
GAAGGTTATGGAGAACTGTG+TGG - chr1_3:73196091-73196110 Msa0139530:intron 45.0%
GTGGCACACTTGAAATCAGT+TGG - chr1_3:73196110-73196129 Msa0139530:intron 45.0%
GCACCAGGTGGAAAATTAAC+AGG + chr1_3:73196191-73196210 None:intergenic 45.0%
TTCTACAGGCAATCACCTGA+TGG - chr1_3:73196218-73196237 Msa0139530:intron 45.0%
! GCAGTGTTGGAGACTATGAT+CGG - chr1_3:73196447-73196466 Msa0139530:CDS 45.0%
CAAACAGCGCTTAAGATCAG+GGG + chr1_3:73196487-73196506 None:intergenic 45.0%
CCAAACAGCGCTTAAGATCA+GGG + chr1_3:73196488-73196507 None:intergenic 45.0%
ATTACCTGCGTTGTTGAACG+AGG - chr1_3:73196515-73196534 Msa0139530:CDS 45.0%
TTACCTGCGTTGTTGAACGA+GGG - chr1_3:73196516-73196535 Msa0139530:CDS 45.0%
!! AACGAGGGTGCTTTCAAGAT+TGG - chr1_3:73196531-73196550 Msa0139530:CDS 45.0%
! TTCTTGTGCTGCAACGCTTT+TGG + chr1_3:73196594-73196613 None:intergenic 45.0%
GAACGAAATAAACGCCGCCT+GGG - chr1_3:73195152-73195171 Msa0139530:three_prime_UTR 50.0%
GGAGAACCTTGAATACCCAG+CGG - chr1_3:73195302-73195321 Msa0139530:three_prime_UTR 50.0%
TAACCACCGCTGGGTATTCA+AGG + chr1_3:73195311-73195330 None:intergenic 50.0%
TGGCTTCACTAGCTGCCAAA+TGG - chr1_3:73195448-73195467 Msa0139530:CDS 50.0%
GACAGACACTCCTCAATCCT+CGG + chr1_3:73195671-73195690 None:intergenic 50.0%
TGAGTGGTCCTGAGTACTCA+AGG - chr1_3:73196069-73196088 Msa0139530:intron 50.0%
TGGTCCTGAGTACTCAAGGA+AGG - chr1_3:73196073-73196092 Msa0139530:intron 50.0%
!! TAGAAAACAGAGGCACCAGG+TGG + chr1_3:73196203-73196222 None:intergenic 50.0%
!! CTGTAGAAAACAGAGGCACC+AGG + chr1_3:73196206-73196225 None:intergenic 50.0%
! CTGGTGCCTCTGTTTTCTAC+AGG - chr1_3:73196204-73196223 Msa0139530:intron 50.0%
! ATGAGTGCAGCCTCCTTTTC+TGG + chr1_3:73196405-73196424 None:intergenic 50.0%
GGCTGCACTCATAGAGAACA+AGG - chr1_3:73196413-73196432 Msa0139530:CDS 50.0%
GGCAGTTTCATCAGCAGTGT+TGG - chr1_3:73196434-73196453 Msa0139530:CDS 50.0%
! CCCTGATCTTAAGCGCTGTT+TGG - chr1_3:73196485-73196504 Msa0139530:CDS 50.0%
GCCAAACAGCGCTTAAGATC+AGG + chr1_3:73196489-73196508 None:intergenic 50.0%
GCAGCACAAGAATGAGACCA+TGG - chr1_3:73196602-73196621 Msa0139530:CDS 50.0%
GAACGAAATAAACGCCGCCT+GGG - chr1_3:73195152-73195171 Msa0139530:three_prime_UTR 50.0%
GGAGAACCTTGAATACCCAG+CGG - chr1_3:73195302-73195321 Msa0139530:three_prime_UTR 50.0%
TAACCACCGCTGGGTATTCA+AGG + chr1_3:73195311-73195330 None:intergenic 50.0%
TGGCTTCACTAGCTGCCAAA+TGG - chr1_3:73195448-73195467 Msa0139530:CDS 50.0%
GACAGACACTCCTCAATCCT+CGG + chr1_3:73195671-73195690 None:intergenic 50.0%
TGAGTGGTCCTGAGTACTCA+AGG - chr1_3:73196069-73196088 Msa0139530:intron 50.0%
TGGTCCTGAGTACTCAAGGA+AGG - chr1_3:73196073-73196092 Msa0139530:intron 50.0%
!! TAGAAAACAGAGGCACCAGG+TGG + chr1_3:73196203-73196222 None:intergenic 50.0%
!! CTGTAGAAAACAGAGGCACC+AGG + chr1_3:73196206-73196225 None:intergenic 50.0%
! CTGGTGCCTCTGTTTTCTAC+AGG - chr1_3:73196204-73196223 Msa0139530:intron 50.0%
! ATGAGTGCAGCCTCCTTTTC+TGG + chr1_3:73196405-73196424 None:intergenic 50.0%
GGCTGCACTCATAGAGAACA+AGG - chr1_3:73196413-73196432 Msa0139530:CDS 50.0%
GGCAGTTTCATCAGCAGTGT+TGG - chr1_3:73196434-73196453 Msa0139530:CDS 50.0%
! CCCTGATCTTAAGCGCTGTT+TGG - chr1_3:73196485-73196504 Msa0139530:CDS 50.0%
GCCAAACAGCGCTTAAGATC+AGG + chr1_3:73196489-73196508 None:intergenic 50.0%
GCAGCACAAGAATGAGACCA+TGG - chr1_3:73196602-73196621 Msa0139530:CDS 50.0%
GGAACGAAATAAACGCCGCC+TGG - chr1_3:73195151-73195170 Msa0139530:three_prime_UTR 55.0%
GAACCTTGAATACCCAGCGG+TGG - chr1_3:73195305-73195324 Msa0139530:three_prime_UTR 55.0%
TCATATTTCCTCGGTGGCGC+TGG - chr1_3:73195351-73195370 Msa0139530:three_prime_UTR 55.0%
CATATTTCCTCGGTGGCGCT+GGG - chr1_3:73195352-73195371 Msa0139530:three_prime_UTR 55.0%
ATATTTCCTCGGTGGCGCTG+GGG - chr1_3:73195353-73195372 Msa0139530:three_prime_UTR 55.0%
GACACATGTCCTCTGATCCG+AGG - chr1_3:73195651-73195670 Msa0139530:intron 55.0%
GTCCTCTGATCCGAGGATTG+AGG - chr1_3:73195658-73195677 Msa0139530:intron 55.0%
CTCCTCAATCCTCGGATCAG+AGG + chr1_3:73195663-73195682 None:intergenic 55.0%
GGAACGAAATAAACGCCGCC+TGG - chr1_3:73195151-73195170 Msa0139530:three_prime_UTR 55.0%
GAACCTTGAATACCCAGCGG+TGG - chr1_3:73195305-73195324 Msa0139530:three_prime_UTR 55.0%
TCATATTTCCTCGGTGGCGC+TGG - chr1_3:73195351-73195370 Msa0139530:three_prime_UTR 55.0%
CATATTTCCTCGGTGGCGCT+GGG - chr1_3:73195352-73195371 Msa0139530:three_prime_UTR 55.0%
ATATTTCCTCGGTGGCGCTG+GGG - chr1_3:73195353-73195372 Msa0139530:three_prime_UTR 55.0%
GACACATGTCCTCTGATCCG+AGG - chr1_3:73195651-73195670 Msa0139530:intron 55.0%
GTCCTCTGATCCGAGGATTG+AGG - chr1_3:73195658-73195677 Msa0139530:intron 55.0%
CTCCTCAATCCTCGGATCAG+AGG + chr1_3:73195663-73195682 None:intergenic 55.0%
AATAGGAAGCGGTCTGGCCC+AGG + chr1_3:73195172-73195191 None:intergenic 60.0%
GTGGTTACCTCTCCGGTCAC+CGG - chr1_3:73195324-73195343 Msa0139530:three_prime_UTR 60.0%
! TGATTTGCCGGTGACCGGAG+AGG + chr1_3:73195334-73195353 None:intergenic 60.0%
GCACCCTCGTTCAACAACGC+AGG + chr1_3:73196522-73196541 None:intergenic 60.0%
AATAGGAAGCGGTCTGGCCC+AGG + chr1_3:73195172-73195191 None:intergenic 60.0%
GTGGTTACCTCTCCGGTCAC+CGG - chr1_3:73195324-73195343 Msa0139530:three_prime_UTR 60.0%
! TGATTTGCCGGTGACCGGAG+AGG + chr1_3:73195334-73195353 None:intergenic 60.0%
GCACCCTCGTTCAACAACGC+AGG + chr1_3:73196522-73196541 None:intergenic 60.0%
CCGGAGAGGTAACCACCGCT+GGG + chr1_3:73195320-73195339 None:intergenic 65.0%
CCCAGCGGTGGTTACCTCTC+CGG - chr1_3:73195317-73195336 Msa0139530:three_prime_UTR 65.0%
ACCGGAGAGGTAACCACCGC+TGG + chr1_3:73195321-73195340 None:intergenic 65.0%
CCGGAGAGGTAACCACCGCT+GGG + chr1_3:73195320-73195339 None:intergenic 65.0%
CCCAGCGGTGGTTACCTCTC+CGG - chr1_3:73195317-73195336 Msa0139530:three_prime_UTR 65.0%
ACCGGAGAGGTAACCACCGC+TGG + chr1_3:73195321-73195340 None:intergenic 65.0%
AGGAAGCGGTCTGGCCCAGG+CGG + chr1_3:73195169-73195188 None:intergenic 70.0%
AGGAAGCGGTCTGGCCCAGG+CGG + chr1_3:73195169-73195188 None:intergenic 70.0%
CTCTCTCCCCAGCGCCACCG+AGG + chr1_3:73195362-73195381 None:intergenic 75.0%
! CTCGGTGGCGCTGGGGAGAG+AGG - chr1_3:73195360-73195379 Msa0139530:exon 75.0%
CTCTCTCCCCAGCGCCACCG+AGG + chr1_3:73195362-73195381 None:intergenic 75.0%
! CTCGGTGGCGCTGGGGAGAG+AGG - chr1_3:73195360-73195379 Msa0139530:exon 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 73195091 73196844 73195091 ID=Msa0139530;Name=Msa0139530
chr1_3 mRNA 73195091 73196844 73195091 ID=Msa0139530-mRNA-1;Parent=Msa0139530;Name=Msa0139530-mRNA-1;_AED=0.41;_eAED=0.41;_QI=182|1|1|1|1|1|3|284|222
chr1_3 exon 73195091 73195560 73195091 ID=Msa0139530-mRNA-1:exon:25564;Parent=Msa0139530-mRNA-1
chr1_3 exon 73195685 73195908 73195685 ID=Msa0139530-mRNA-1:exon:25563;Parent=Msa0139530-mRNA-1
chr1_3 exon 73196404 73196844 73196404 ID=Msa0139530-mRNA-1:exon:25562;Parent=Msa0139530-mRNA-1
chr1_3 five_prime_UTR 73196663 73196844 73196663 ID=Msa0139530-mRNA-1:five_prime_utr;Parent=Msa0139530-mRNA-1
chr1_3 CDS 73196404 73196662 73196404 ID=Msa0139530-mRNA-1:cds;Parent=Msa0139530-mRNA-1
chr1_3 CDS 73195685 73195908 73195685 ID=Msa0139530-mRNA-1:cds;Parent=Msa0139530-mRNA-1
chr1_3 CDS 73195375 73195560 73195375 ID=Msa0139530-mRNA-1:cds;Parent=Msa0139530-mRNA-1
chr1_3 three_prime_UTR 73195091 73195374 73195091 ID=Msa0139530-mRNA-1:three_prime_utr;Parent=Msa0139530-mRNA-1
Gene Sequence

>Msa0139530

ATGGCTGCATCAATCACCGCAATCACTGTGGAGAACCTTGAATACCCAGCGGTGGTTACCTCTCCGGTCACCGGCAAATCATATTTCCTCGGTGGCGCTGGGGAGAGAGGATTGACCATTGAAGGAAACTTCATCAAGTTCACTGCCATAGGTGTTTATTTGGAAGATATAGCAGTGGCTTCACTAGCTGCCAAATGGAAGGGTAAATCATCTGAAGAGTTACTTGAGACCCTCGACTTTTACAGAGACATCATCTCAGGTCCCTTTGAAAAGTTAATTAGAGGGTCAAAGATTAGGGAATTGAGTGGTCCTGAGTACTCAAGGAAGGTTATGGAGAACTGTGTGGCACACTTGAAATCAGTTGGAACTTATGGAGATGCAGAAGCTGAAGCTATGCAAAAATTTGCTGAAGCTTTCAAGCCTGTTAATTTTCCACCTGGTGCCTCTGTTTTCTACAGGCAATCACCTGATGGAATATTAGGGCTTAGTTTCTCTCCGGATACAAGTATACCAGAAAAGGAGGCTGCACTCATAGAGAACAAGGCAGTTTCATCAGCAGTGTTGGAGACTATGATCGGCGAACACGCTGTTTCCCCTGATCTTAAGCGCTGTTTGGCTGCAAGATTACCTGCGTTGTTGAACGAGGGTGCTTTCAAGATTGGAAACTGA
Protein sequence

>Msa0139530

MAASITAITVENLEYPAVVTSPVTGKSYFLGGAGERGLTIEGNFIKFTAIGVYLEDIAVASLAAKWKGKSSEELLETLDFYRDIISGPFEKLIRGSKIRELSGPEYSRKVMENCVAHLKSVGTYGDAEAEAMQKFAEAFKPVNFPPGASVFYRQSPDGILGLSFSPDTSIPEKEAALIENKAVSSAVLETMIGEHAVSPDLKRCLAARLPALLNEGAFKIGN*