AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1009990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1009990 MtrunA17_Chr7g0220791 100.000 52 0 0 1 52 1 52 3.04e-32 104
Msa1009990 MtrunA17_Chr7g0220801 100.000 52 0 0 1 52 1 52 3.04e-32 104
Msa1009990 MtrunA17_Chr7g0220831 96.154 52 2 0 1 52 1 52 7.93e-31 101
Msa1009990 MtrunA17_Chr4g0020171 96.154 52 2 0 1 52 1 52 7.93e-31 101
Msa1009990 MtrunA17_Chr8g0368651 76.923 52 11 1 1 52 1 51 5.37e-17 66.2
Msa1009990 MtrunA17_Chr4g0021531 75.000 36 9 0 17 52 19 54 3.19e-16 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 94 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAAATAAATAAAGTATT+TGG 0.157187 7_1:+12472679 Msa1009990:three_prime_UTR
ACAAAAGCTCAAATTTATTT+AGG 0.194551 7_1:-12472580 None:intergenic
AAGTCGCAAATGTCAGATTT+TGG 0.226019 7_1:-12472102 None:intergenic
AAATTTCCTTTGGAGGTTAA+AGG 0.271383 7_1:+12472351 Msa1009990:three_prime_UTR
TTCTCTGATCCTGCTGGATT+AGG 0.281394 7_1:+12471526 Msa1009990:three_prime_UTR
GCTGTTCTTGACAGCTATTT+TGG 0.313748 7_1:+12471853 Msa1009990:three_prime_UTR
AAGGAGAGAGGACAAGAAAA+AGG 0.318811 7_1:-12471383 None:intergenic
TGGGTTGTGATATAGTTCTT+AGG 0.327149 7_1:+12471322 Msa1009990:three_prime_UTR
GTTTCAGAATCTCGTCAATT+TGG 0.331728 7_1:-12471570 None:intergenic
GGTTTGATGACTAAGAATTC+TGG 0.333324 7_1:+12472063 Msa1009990:three_prime_UTR
GCATAACTGAATCCATCTTC+AGG 0.337519 7_1:-12472195 None:intergenic
TGAGAACGAGGAGGAAGTTA+AGG 0.347960 7_1:+12472500 Msa1009990:three_prime_UTR
ATGCTGATAAGTCACAAATT+TGG 0.357352 7_1:+12471914 Msa1009990:three_prime_UTR
CGTACCTTTGTTCGAAATGA+TGG 0.360403 7_1:+12472432 Msa1009990:three_prime_UTR
AAAACTTGACAAATTTCCTT+TGG 0.369192 7_1:+12472341 Msa1009990:three_prime_UTR
AAGAGGAGCTTCGTACAAAA+AGG 0.380347 7_1:-12471082 None:intergenic
GCTTCACCGGAAGCTGTCTA+TGG 0.396357 7_1:+12471967 Msa1009990:three_prime_UTR
ACTTTGAATTTGAACCTTGC+GGG 0.397559 7_1:+12472121 Msa1009990:three_prime_UTR
TCAAGGCCATAGACAGCTTC+CGG 0.398954 7_1:-12471973 None:intergenic
TCGGCAAGCTTAAGAATTGC+AGG 0.407592 7_1:-12471730 None:intergenic
AAAATCTCAGGATGGTTTGC+GGG 0.408047 7_1:-12471298 None:intergenic
AAATCTCTGAATGAACTTGT+TGG 0.430429 7_1:+12472252 Msa1009990:three_prime_UTR
TGAGGGAATGATTGAGCTCC+AGG 0.436069 7_1:-12471808 None:intergenic
AACCAGTCATGCACATTTCA+AGG 0.440811 7_1:-12471990 None:intergenic
AATCTCGTCAATTTGGTCTC+TGG 0.441714 7_1:-12471563 None:intergenic
ACTTGACAAATTTCCTTTGG+AGG 0.442578 7_1:+12472344 Msa1009990:three_prime_UTR
TGACTAAGAATTCTGGAATC+AGG 0.442912 7_1:+12472070 Msa1009990:three_prime_UTR
ATCGAAGACGTTCGACCAAA+CGG 0.449223 7_1:+12471114 Msa1009990:CDS
TACTCGATGAACGGAATTGA+AGG 0.451132 7_1:+12472144 Msa1009990:three_prime_UTR
ATCCATGTCACCCCTGAAGA+TGG 0.462649 7_1:+12472183 Msa1009990:three_prime_UTR
GGCCTTGAAATGTGCATGAC+TGG 0.467615 7_1:+12471988 Msa1009990:three_prime_UTR
TTCAGGGGTGACATGGATAG+TGG 0.468582 7_1:-12472178 None:intergenic
AAGACAGATGCATCTTCAGC+AGG 0.478706 7_1:+12472042 Msa1009990:three_prime_UTR
CATGGTCATCTTGACTCAAA+GGG 0.480565 7_1:-12471434 None:intergenic
CTCTGAATGAACTTGTTGGA+AGG 0.494644 7_1:+12472256 Msa1009990:three_prime_UTR
TCAACAATGGTGCATTCTGC+TGG 0.497159 7_1:-12471592 None:intergenic
TCTGAATGAACTTGTTGGAA+GGG 0.497768 7_1:+12472257 Msa1009990:three_prime_UTR
CATAACTGAATCCATCTTCA+GGG 0.502683 7_1:-12472194 None:intergenic
GGTCTACTCTGAAATGCTGC+TGG 0.506003 7_1:+12472469 Msa1009990:three_prime_UTR
TTTGGTCTCTGGACAATGCT+CGG 0.511847 7_1:-12471552 None:intergenic
TCTTAACATTGCTGTGAAAT+CGG 0.512952 7_1:+12471756 Msa1009990:three_prime_UTR
GAGAGGATCAATGAAGTGCT+TGG 0.514175 7_1:+12472387 Msa1009990:three_prime_UTR
AAGGTGTGTGAATGAACTAA+TGG 0.520867 7_1:+12471017 Msa1009990:exon
GTTTGTAGAATTAAGGAGAG+AGG 0.521164 7_1:-12471395 None:intergenic
CACAGCAATGTTAAGACCAT+CGG 0.522381 7_1:-12471749 None:intergenic
TCATGGTCATCTTGACTCAA+AGG 0.522835 7_1:-12471435 None:intergenic
TTTATTTAGAAATATAATCC+AGG 0.523545 7_1:-12472667 None:intergenic
CAAATTTATTTAGGTAACAA+TGG 0.523956 7_1:-12472571 None:intergenic
TGGTAGCCAAGCTTATATGA+TGG 0.524204 7_1:+12471888 Msa1009990:three_prime_UTR
CAGCATTTCAGAGTAGACCT+TGG 0.524481 7_1:-12472465 None:intergenic
GACTGGTTTAGATAAAGAAA+AGG 0.528480 7_1:+12472005 Msa1009990:three_prime_UTR
TTGTATCCTTTAACCTCCAA+AGG 0.534196 7_1:-12472357 None:intergenic
GTCAAGAACAGCAACTTCCT+CGG 0.536338 7_1:-12471842 None:intergenic
AAGCAATTTCGTAGTCCCAC+AGG 0.538583 7_1:-12471698 None:intergenic
ATCCATCTTCAGGGGTGACA+TGG 0.542033 7_1:-12472185 None:intergenic
TGCTAAGCTAGACGCTTCAC+CGG 0.556532 7_1:+12471954 Msa1009990:three_prime_UTR
CATCACCCATCATATAAGCT+TGG 0.565152 7_1:-12471894 None:intergenic
TGTTGATTCATATGTCCTAT+CGG 0.565465 7_1:+12471633 Msa1009990:three_prime_UTR
GCTCGGAGGCCTAATCCAGC+AGG 0.569852 7_1:-12471535 None:intergenic
CCGTTCATCGAGTACCCGCA+AGG 0.572227 7_1:-12472135 None:intergenic
TTATGCAAGCTTTGAAGCAG+TGG 0.572426 7_1:+12472212 Msa1009990:three_prime_UTR
TATGCAAGCTTTGAAGCAGT+GGG 0.574688 7_1:+12472213 Msa1009990:three_prime_UTR
AGCTTATCATCAAAACCTGT+GGG 0.576476 7_1:+12471683 Msa1009990:three_prime_UTR
GGTAGCCAAGCTTATATGAT+GGG 0.582473 7_1:+12471889 Msa1009990:three_prime_UTR
AATGAAGTGCTTGGAGAAGA+CGG 0.582790 7_1:+12472396 Msa1009990:three_prime_UTR
AATGAACTAATGGAGTCAAA+AGG 0.584923 7_1:+12471027 Msa1009990:exon
GCAATTCTTAAGCTTGCCGA+TGG 0.593187 7_1:+12471733 Msa1009990:three_prime_UTR
ATGATGGCTGCTCATCTCCA+AGG 0.593841 7_1:+12472448 Msa1009990:three_prime_UTR
ACCATGACAAACTCAGCTAT+CGG 0.606560 7_1:+12471451 Msa1009990:three_prime_UTR
GACTTTGAATTTGAACCTTG+CGG 0.607803 7_1:+12472120 Msa1009990:three_prime_UTR
AATGCAACAGAAAACTCAGC+TGG 0.608895 7_1:-12472297 None:intergenic
TGAATAAGCTTGACTCCGAT+AGG 0.610322 7_1:-12471648 None:intergenic
AAGCTTATCATCAAAACCTG+TGG 0.612735 7_1:+12471682 Msa1009990:three_prime_UTR
ACCGATAGCTGAGTTTGTCA+TGG 0.615180 7_1:-12471452 None:intergenic
CCTCGGAAAAGCTTCGATGA+GGG 0.618203 7_1:-12471825 None:intergenic
TCCTCGGAAAAGCTTCGATG+AGG 0.618294 7_1:-12471826 None:intergenic
CCTTGCGGGTACTCGATGAA+CGG 0.629304 7_1:+12472135 Msa1009990:three_prime_UTR
TCTGAAATGCTGCTGGAGTG+AGG 0.630987 7_1:+12472476 Msa1009990:three_prime_UTR
AAGGATACAACTGTGGAGAG+AGG 0.638648 7_1:+12472370 Msa1009990:three_prime_UTR
ATTAGATAGTGAGTCAACAA+TGG 0.640442 7_1:-12471605 None:intergenic
CATCATTTCGAACAAAGGTA+CGG 0.642356 7_1:-12472431 None:intergenic
GAACTAATGGAGTCAAAAGG+TGG 0.643029 7_1:+12471030 Msa1009990:exon
CGGCAAGCTTAAGAATTGCA+GGG 0.643796 7_1:-12471729 None:intergenic
CTGGAGTGAGGATGAGAACG+AGG 0.646055 7_1:+12472488 Msa1009990:three_prime_UTR
GCAGCCATCATTTCGAACAA+AGG 0.652571 7_1:-12472436 None:intergenic
GCATTGCACATTGATGCGAA+TGG 0.665215 7_1:+12472315 Msa1009990:three_prime_UTR
ATGGTCATCTTGACTCAAAG+GGG 0.671726 7_1:-12471433 None:intergenic
GAGGTTAAAGGATACAACTG+TGG 0.681618 7_1:+12472363 Msa1009990:three_prime_UTR
GGTCTCTGGACAATGCTCGG+AGG 0.685264 7_1:-12471549 None:intergenic
ATAACTGAATCCATCTTCAG+GGG 0.689618 7_1:-12472193 None:intergenic
GAGTGAGGATGAGAACGAGG+AGG 0.703166 7_1:+12472491 Msa1009990:three_prime_UTR
TCTTCGATGATGTAACCAAG+AGG 0.710301 7_1:-12471099 None:intergenic
GAAGACGTTCGACCAAACGG+TGG 0.758617 7_1:+12471117 Msa1009990:CDS
AAATCGGTGAGATACACTCG+CGG 0.764779 7_1:+12471772 Msa1009990:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTCTTAGATTATTGATTA+TGG + chr7_1:12470428-12470447 Msa1009990:intron 15.0%
!! TTTCAATAAAAAAAACAGAT+AGG - chr7_1:12470555-12470574 None:intergenic 15.0%
!!! TGAAGTTTATAATCAATTTT+AGG - chr7_1:12470628-12470647 None:intergenic 15.0%
!! TTTATTTAGAAATATAATCC+AGG - chr7_1:12472670-12472689 None:intergenic 15.0%
!! AATTGAGAAAAAAACACATA+AGG - chr7_1:12470245-12470264 None:intergenic 20.0%
!!! TTTTTGATATTGTTCGATTT+AGG + chr7_1:12470304-12470323 Msa1009990:intron 20.0%
!!! ACTTACAGTTTTTAAACAAT+TGG - chr7_1:12470389-12470408 None:intergenic 20.0%
!!! TAGATTATTGATTATGGTTT+TGG + chr7_1:12470434-12470453 Msa1009990:intron 20.0%
!!! AAAATTTCCCTTTTTCTTTT+CGG + chr7_1:12470572-12470591 Msa1009990:intron 20.0%
!! AAACAAGAAGGTAAAAAAAT+TGG - chr7_1:12470885-12470904 None:intergenic 20.0%
!! ATGATGTTAACATTAATCAA+AGG - chr7_1:12471208-12471227 None:intergenic 20.0%
!! CATAGTATATAGTATAAGAT+TGG + chr7_1:12471225-12471244 Msa1009990:intron 20.0%
!! ACTGCATAATAAAGAAAAAA+GGG - chr7_1:12471275-12471294 None:intergenic 20.0%
!!! TTTTGTAGTTTGTAGAATTA+AGG - chr7_1:12471405-12471424 None:intergenic 20.0%
!! CAAATTTATTTAGGTAACAA+TGG - chr7_1:12472574-12472593 None:intergenic 20.0%
!! ACAAAAGCTCAAATTTATTT+AGG - chr7_1:12472583-12472602 None:intergenic 20.0%
! TCAGAATTCTGATTCATAAA+AGG + chr7_1:12470337-12470356 Msa1009990:intron 25.0%
! CAGAATTCTGATTCATAAAA+GGG + chr7_1:12470338-12470357 Msa1009990:intron 25.0%
! AGAATTCTGATTCATAAAAG+GGG + chr7_1:12470339-12470358 Msa1009990:intron 25.0%
! AAACAAACCGAAAAGAAAAA+GGG - chr7_1:12470582-12470601 None:intergenic 25.0%
! AAAACAAACCGAAAAGAAAA+AGG - chr7_1:12470583-12470602 None:intergenic 25.0%
! AACAACACATACATCAAAAT+TGG - chr7_1:12470807-12470826 None:intergenic 25.0%
! GAAGGTAAAAAAATTGGAAT+TGG - chr7_1:12470879-12470898 None:intergenic 25.0%
! AACAATATGTTCAAACAAGA+AGG - chr7_1:12470897-12470916 None:intergenic 25.0%
! TAGTATAAGATTGGATGATT+TGG + chr7_1:12471234-12471253 Msa1009990:intron 25.0%
! CACTGCATAATAAAGAAAAA+AGG - chr7_1:12471276-12471295 None:intergenic 25.0%
! AAAACTTGACAAATTTCCTT+TGG + chr7_1:12472341-12472360 Msa1009990:three_prime_UTR 25.0%
CAGAGAATGAAGAAAAATTG+AGG - chr7_1:12470204-12470223 None:intergenic 30.0%
!!! TGGTTTTGGATGAAGAAATA+TGG + chr7_1:12470448-12470467 Msa1009990:intron 30.0%
!!! ATTTTGTGAAGAGCATTTGA+TGG + chr7_1:12470523-12470542 Msa1009990:intron 30.0%
GTTGAAAATTGACACAACTA+TGG - chr7_1:12470743-12470762 None:intergenic 30.0%
!! CAACTTGTTAAGAAATTGAC+TGG + chr7_1:12470774-12470793 Msa1009990:intron 30.0%
GCAAAGAAATCATGATTTCT+GGG - chr7_1:12470947-12470966 None:intergenic 30.0%
TGCAAAGAAATCATGATTTC+TGG - chr7_1:12470948-12470967 None:intergenic 30.0%
! GCTTCATTCTTTTAACAGAA+AGG + chr7_1:12470998-12471017 Msa1009990:intron 30.0%
AATGAACTAATGGAGTCAAA+AGG + chr7_1:12471027-12471046 Msa1009990:exon 30.0%
!!! AAACCATCCTGAGATTTTTT+GGG + chr7_1:12471303-12471322 Msa1009990:exon 30.0%
!!! GAGACAACTTTTTTCGAAAA+CGG + chr7_1:12471499-12471518 Msa1009990:three_prime_UTR 30.0%
ATTAGATAGTGAGTCAACAA+TGG - chr7_1:12471608-12471627 None:intergenic 30.0%
!! TGTTGATTCATATGTCCTAT+CGG + chr7_1:12471633-12471652 Msa1009990:three_prime_UTR 30.0%
TCTTAACATTGCTGTGAAAT+CGG + chr7_1:12471756-12471775 Msa1009990:three_prime_UTR 30.0%
ATGCTGATAAGTCACAAATT+TGG + chr7_1:12471914-12471933 Msa1009990:three_prime_UTR 30.0%
!! GACTGGTTTAGATAAAGAAA+AGG + chr7_1:12472005-12472024 Msa1009990:three_prime_UTR 30.0%
AAATCTCTGAATGAACTTGT+TGG + chr7_1:12472252-12472271 Msa1009990:three_prime_UTR 30.0%
! AAATTTCCTTTGGAGGTTAA+AGG + chr7_1:12472351-12472370 Msa1009990:three_prime_UTR 30.0%
!!! TTTTTTTTGAGTGTTGTACC+TGG + chr7_1:12472649-12472668 Msa1009990:three_prime_UTR 30.0%
ATAAAAATGCCACCCAGATT+TGG + chr7_1:12470715-12470734 Msa1009990:intron 35.0%
ACAACTATGGAACCAAATCT+GGG - chr7_1:12470730-12470749 None:intergenic 35.0%
AAGGTGTGTGAATGAACTAA+TGG + chr7_1:12471017-12471036 Msa1009990:exon 35.0%
AAAAATCTCAGGATGGTTTG+CGG - chr7_1:12471302-12471321 None:intergenic 35.0%
!!! CAAACCATCCTGAGATTTTT+TGG + chr7_1:12471302-12471321 Msa1009990:exon 35.0%
ATCACAACCCAAAAAATCTC+AGG - chr7_1:12471313-12471332 None:intergenic 35.0%
TGGGTTGTGATATAGTTCTT+AGG + chr7_1:12471322-12471341 Msa1009990:three_prime_UTR 35.0%
GTTTGTAGAATTAAGGAGAG+AGG - chr7_1:12471398-12471417 None:intergenic 35.0%
!!! GTTTTGAAGGCTATGAAAAG+AGG + chr7_1:12471473-12471492 Msa1009990:three_prime_UTR 35.0%
GTTTCAGAATCTCGTCAATT+TGG - chr7_1:12471573-12471592 None:intergenic 35.0%
AAGCTTATCATCAAAACCTG+TGG + chr7_1:12471682-12471701 Msa1009990:three_prime_UTR 35.0%
AGCTTATCATCAAAACCTGT+GGG + chr7_1:12471683-12471702 Msa1009990:three_prime_UTR 35.0%
! GGTTTGATGACTAAGAATTC+TGG + chr7_1:12472063-12472082 Msa1009990:three_prime_UTR 35.0%
!! TGACTAAGAATTCTGGAATC+AGG + chr7_1:12472070-12472089 Msa1009990:three_prime_UTR 35.0%
! AAGTCGCAAATGTCAGATTT+TGG - chr7_1:12472105-12472124 None:intergenic 35.0%
GACTTTGAATTTGAACCTTG+CGG + chr7_1:12472120-12472139 Msa1009990:three_prime_UTR 35.0%
ACTTTGAATTTGAACCTTGC+GGG + chr7_1:12472121-12472140 Msa1009990:three_prime_UTR 35.0%
ATAACTGAATCCATCTTCAG+GGG - chr7_1:12472196-12472215 None:intergenic 35.0%
CATAACTGAATCCATCTTCA+GGG - chr7_1:12472197-12472216 None:intergenic 35.0%
!! TCTGAATGAACTTGTTGGAA+GGG + chr7_1:12472257-12472276 Msa1009990:three_prime_UTR 35.0%
ACTTGACAAATTTCCTTTGG+AGG + chr7_1:12472344-12472363 Msa1009990:three_prime_UTR 35.0%
TTGTATCCTTTAACCTCCAA+AGG - chr7_1:12472360-12472379 None:intergenic 35.0%
CATCATTTCGAACAAAGGTA+CGG - chr7_1:12472434-12472453 None:intergenic 35.0%
! GGAAATCGAGAAGCATAGAT+CGG + chr7_1:12470360-12470379 Msa1009990:intron 40.0%
CACAACTATGGAACCAAATC+TGG - chr7_1:12470731-12470750 None:intergenic 40.0%
GAACTAATGGAGTCAAAAGG+TGG + chr7_1:12471030-12471049 Msa1009990:exon 40.0%
AAGAGGAGCTTCGTACAAAA+AGG - chr7_1:12471085-12471104 None:intergenic 40.0%
! TTTTTGTACGAAGCTCCTCT+TGG + chr7_1:12471084-12471103 Msa1009990:CDS 40.0%
TCTTCGATGATGTAACCAAG+AGG - chr7_1:12471102-12471121 None:intergenic 40.0%
! AATTTTTCTACGCCACCGTT+TGG - chr7_1:12471132-12471151 None:intergenic 40.0%
! AAAATCTCAGGATGGTTTGC+GGG - chr7_1:12471301-12471320 None:intergenic 40.0%
CAACCCAAAAAATCTCAGGA+TGG - chr7_1:12471309-12471328 None:intergenic 40.0%
AAGGAGAGAGGACAAGAAAA+AGG - chr7_1:12471386-12471405 None:intergenic 40.0%
ATGGTCATCTTGACTCAAAG+GGG - chr7_1:12471436-12471455 None:intergenic 40.0%
CATGGTCATCTTGACTCAAA+GGG - chr7_1:12471437-12471456 None:intergenic 40.0%
TCATGGTCATCTTGACTCAA+AGG - chr7_1:12471438-12471457 None:intergenic 40.0%
ACCATGACAAACTCAGCTAT+CGG + chr7_1:12471451-12471470 Msa1009990:three_prime_UTR 40.0%
!!! AACTCAGCTATCGGTTTTGA+AGG + chr7_1:12471460-12471479 Msa1009990:three_prime_UTR 40.0%
AATCTCGTCAATTTGGTCTC+TGG - chr7_1:12471566-12471585 None:intergenic 40.0%
TGAATAAGCTTGACTCCGAT+AGG - chr7_1:12471651-12471670 None:intergenic 40.0%
CACAGCAATGTTAAGACCAT+CGG - chr7_1:12471752-12471771 None:intergenic 40.0%
! GCTGTTCTTGACAGCTATTT+TGG + chr7_1:12471853-12471872 Msa1009990:three_prime_UTR 40.0%
!!! TATTTTGGCGAACTTGGTTC+TGG + chr7_1:12471868-12471887 Msa1009990:three_prime_UTR 40.0%
TGGTAGCCAAGCTTATATGA+TGG + chr7_1:12471888-12471907 Msa1009990:three_prime_UTR 40.0%
GGTAGCCAAGCTTATATGAT+GGG + chr7_1:12471889-12471908 Msa1009990:three_prime_UTR 40.0%
CATCACCCATCATATAAGCT+TGG - chr7_1:12471897-12471916 None:intergenic 40.0%
AACCAGTCATGCACATTTCA+AGG - chr7_1:12471993-12472012 None:intergenic 40.0%
! CGCAAATGTCAGATTTTGGA+AGG - chr7_1:12472101-12472120 None:intergenic 40.0%
TACTCGATGAACGGAATTGA+AGG + chr7_1:12472144-12472163 Msa1009990:three_prime_UTR 40.0%
GCATAACTGAATCCATCTTC+AGG - chr7_1:12472198-12472217 None:intergenic 40.0%
!! TTATGCAAGCTTTGAAGCAG+TGG + chr7_1:12472212-12472231 Msa1009990:three_prime_UTR 40.0%
!! TATGCAAGCTTTGAAGCAGT+GGG + chr7_1:12472213-12472232 Msa1009990:three_prime_UTR 40.0%
!! CTCTGAATGAACTTGTTGGA+AGG + chr7_1:12472256-12472275 Msa1009990:three_prime_UTR 40.0%
AATGCAACAGAAAACTCAGC+TGG - chr7_1:12472300-12472319 None:intergenic 40.0%
GAGGTTAAAGGATACAACTG+TGG + chr7_1:12472363-12472382 Msa1009990:three_prime_UTR 40.0%
! AATGAAGTGCTTGGAGAAGA+CGG + chr7_1:12472396-12472415 Msa1009990:three_prime_UTR 40.0%
CGTACCTTTGTTCGAAATGA+TGG + chr7_1:12472432-12472451 Msa1009990:three_prime_UTR 40.0%
ACTATGGAACCAAATCTGGG+TGG - chr7_1:12470727-12470746 None:intergenic 45.0%
AGGAGCTTCGTACAAAAAGG+AGG - chr7_1:12471082-12471101 None:intergenic 45.0%
ATCGAAGACGTTCGACCAAA+CGG + chr7_1:12471114-12471133 Msa1009990:CDS 45.0%
AGGACAAGAAAAAGGAGAGG+AGG - chr7_1:12471378-12471397 None:intergenic 45.0%
GAGAGGACAAGAAAAAGGAG+AGG - chr7_1:12471381-12471400 None:intergenic 45.0%
ACCGATAGCTGAGTTTGTCA+TGG - chr7_1:12471455-12471474 None:intergenic 45.0%
TTCTCTGATCCTGCTGGATT+AGG + chr7_1:12471526-12471545 Msa1009990:three_prime_UTR 45.0%
TTTGGTCTCTGGACAATGCT+CGG - chr7_1:12471555-12471574 None:intergenic 45.0%
TCAACAATGGTGCATTCTGC+TGG - chr7_1:12471595-12471614 None:intergenic 45.0%
AAGCAATTTCGTAGTCCCAC+AGG - chr7_1:12471701-12471720 None:intergenic 45.0%
CGGCAAGCTTAAGAATTGCA+GGG - chr7_1:12471732-12471751 None:intergenic 45.0%
TCGGCAAGCTTAAGAATTGC+AGG - chr7_1:12471733-12471752 None:intergenic 45.0%
GCAATTCTTAAGCTTGCCGA+TGG + chr7_1:12471733-12471752 Msa1009990:three_prime_UTR 45.0%
AAATCGGTGAGATACACTCG+CGG + chr7_1:12471772-12471791 Msa1009990:three_prime_UTR 45.0%
!! CGCGGAAGTTTCATTTTTCC+TGG + chr7_1:12471790-12471809 Msa1009990:three_prime_UTR 45.0%
GTCAAGAACAGCAACTTCCT+CGG - chr7_1:12471845-12471864 None:intergenic 45.0%
!!! GACAGCTATTTTGGCGAACT+TGG + chr7_1:12471862-12471881 Msa1009990:three_prime_UTR 45.0%
AAGACAGATGCATCTTCAGC+AGG + chr7_1:12472042-12472061 Msa1009990:three_prime_UTR 45.0%
GCATTGCACATTGATGCGAA+TGG + chr7_1:12472315-12472334 Msa1009990:three_prime_UTR 45.0%
AAGGATACAACTGTGGAGAG+AGG + chr7_1:12472370-12472389 Msa1009990:three_prime_UTR 45.0%
! GAGAGGATCAATGAAGTGCT+TGG + chr7_1:12472387-12472406 Msa1009990:three_prime_UTR 45.0%
GCAGCCATCATTTCGAACAA+AGG - chr7_1:12472439-12472458 None:intergenic 45.0%
CAGCATTTCAGAGTAGACCT+TGG - chr7_1:12472468-12472487 None:intergenic 45.0%
TGAGAACGAGGAGGAAGTTA+AGG + chr7_1:12472500-12472519 Msa1009990:three_prime_UTR 45.0%
! GGTGTTTTCTCTGATCCTGC+TGG + chr7_1:12471520-12471539 Msa1009990:three_prime_UTR 50.0%
TGAGGGAATGATTGAGCTCC+AGG - chr7_1:12471811-12471830 None:intergenic 50.0%
CCTCGGAAAAGCTTCGATGA+GGG - chr7_1:12471828-12471847 None:intergenic 50.0%
TCCTCGGAAAAGCTTCGATG+AGG - chr7_1:12471829-12471848 None:intergenic 50.0%
TGCTAAGCTAGACGCTTCAC+CGG + chr7_1:12471954-12471973 Msa1009990:three_prime_UTR 50.0%
TCAAGGCCATAGACAGCTTC+CGG - chr7_1:12471976-12471995 None:intergenic 50.0%
GGCCTTGAAATGTGCATGAC+TGG + chr7_1:12471988-12472007 Msa1009990:three_prime_UTR 50.0%
TTCAGGGGTGACATGGATAG+TGG - chr7_1:12472181-12472200 None:intergenic 50.0%
ATCCATGTCACCCCTGAAGA+TGG + chr7_1:12472183-12472202 Msa1009990:three_prime_UTR 50.0%
ATCCATCTTCAGGGGTGACA+TGG - chr7_1:12472188-12472207 None:intergenic 50.0%
ATGATGGCTGCTCATCTCCA+AGG + chr7_1:12472448-12472467 Msa1009990:three_prime_UTR 50.0%
!! GGTCTACTCTGAAATGCTGC+TGG + chr7_1:12472469-12472488 Msa1009990:three_prime_UTR 50.0%
! TCTGAAATGCTGCTGGAGTG+AGG + chr7_1:12472476-12472495 Msa1009990:three_prime_UTR 50.0%
GAAGACGTTCGACCAAACGG+TGG + chr7_1:12471117-12471136 Msa1009990:CDS 55.0%
! CCCTCATCGAAGCTTTTCCG+AGG + chr7_1:12471825-12471844 Msa1009990:three_prime_UTR 55.0%
GCTTCACCGGAAGCTGTCTA+TGG + chr7_1:12471967-12471986 Msa1009990:three_prime_UTR 55.0%
CCTTGCGGGTACTCGATGAA+CGG + chr7_1:12472135-12472154 Msa1009990:three_prime_UTR 55.0%
CTGGAGTGAGGATGAGAACG+AGG + chr7_1:12472488-12472507 Msa1009990:three_prime_UTR 55.0%
GAGTGAGGATGAGAACGAGG+AGG + chr7_1:12472491-12472510 Msa1009990:three_prime_UTR 55.0%
GGTCTCTGGACAATGCTCGG+AGG - chr7_1:12471552-12471571 None:intergenic 60.0%
CCGTTCATCGAGTACCCGCA+AGG - chr7_1:12472138-12472157 None:intergenic 60.0%
GCTCGGAGGCCTAATCCAGC+AGG - chr7_1:12471538-12471557 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 12470156 12472699 12470156 ID=Msa1009990;Name=Msa1009990
chr7_1 mRNA 12470156 12472699 12470156 ID=Msa1009990-mRNA-1;Parent=Msa1009990;Name=Msa1009990-mRNA-1;_AED=0.24;_eAED=0.24;_QI=87|1|1|1|0.5|0.66|3|1385|52
chr7_1 exon 12470156 12470222 12470156 ID=Msa1009990-mRNA-1:exon:3212;Parent=Msa1009990-mRNA-1
chr7_1 exon 12471016 12471173 12471016 ID=Msa1009990-mRNA-1:exon:3213;Parent=Msa1009990-mRNA-1
chr7_1 exon 12471294 12472699 12471294 ID=Msa1009990-mRNA-1:exon:3214;Parent=Msa1009990-mRNA-1
chr7_1 five_prime_UTR 12470156 12470222 12470156 ID=Msa1009990-mRNA-1:five_prime_utr;Parent=Msa1009990-mRNA-1
chr7_1 five_prime_UTR 12471016 12471035 12471016 ID=Msa1009990-mRNA-1:five_prime_utr;Parent=Msa1009990-mRNA-1
chr7_1 CDS 12471036 12471173 12471036 ID=Msa1009990-mRNA-1:cds;Parent=Msa1009990-mRNA-1
chr7_1 CDS 12471294 12471314 12471294 ID=Msa1009990-mRNA-1:cds;Parent=Msa1009990-mRNA-1
chr7_1 three_prime_UTR 12471315 12472699 12471315 ID=Msa1009990-mRNA-1:three_prime_utr;Parent=Msa1009990-mRNA-1
Gene Sequence

>Msa1009990

ATGGAGTCAAAAGGTGGTAAGAAGAAGTCTAGCAGTAGTAGTTCCTCCTTTTTGTACGAAGCTCCTCTTGGTTACATCATCGAAGACGTTCGACCAAACGGTGGCGTAGAAAAATTCAAATCTGCTGATTACTCAAACTGCACCCGCAAACCATCCTGA
Protein sequence

>Msa1009990

MESKGGKKKSSSSSSSFLYEAPLGYIIEDVRPNGGVEKFKSADYSNCTRKPS*