Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043760 | AES59455.1 | 94.000 | 100 | 6 | 0 | 1 | 100 | 1 | 100 | 3.76e-61 | 191 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043760 | G7I3M0 | 94.000 | 100 | 6 | 0 | 1 | 100 | 1 | 100 | 1.79e-61 | 191 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0195100 | Msa1043760 | 0.826779 | 2.249492e-54 | -8.615850e-47 |
| Msa0202800 | Msa1043760 | 0.826857 | 2.155047e-54 | -8.615850e-47 |
| Msa0230320 | Msa1043760 | 0.824228 | 9.042572e-54 | -8.615850e-47 |
| Msa0389770 | Msa1043760 | 0.831537 | 1.578548e-55 | -8.615850e-47 |
| Msa1043760 | Msa1177560 | 0.803768 | 2.978044e-49 | -8.615850e-47 |
| Msa1043760 | Msa1211420 | -0.804214 | 2.405062e-49 | -8.615850e-47 |
| Msa1043760 | Msa1232740 | 0.808338 | 3.252470e-50 | -8.615850e-47 |
| Msa1043760 | Msa1271130 | 0.807784 | 4.268311e-50 | -8.615850e-47 |
| Msa1043760 | Msa1313320 | 0.815695 | 8.103086e-52 | -8.615850e-47 |
| Msa1043760 | Msa1392910 | 0.804441 | 2.157460e-49 | -8.615850e-47 |
| Msa1043760 | Msa1423780 | 0.816837 | 4.500023e-52 | -8.615850e-47 |
| Msa0290870 | Msa1043760 | 0.800087 | 1.699179e-48 | -8.615850e-47 |
| Msa0308630 | Msa1043760 | 0.841623 | 4.245979e-58 | -8.615850e-47 |
| Msa0336320 | Msa1043760 | 0.801338 | 9.437790e-49 | -8.615850e-47 |
| Msa0558370 | Msa1043760 | 0.807513 | 4.872126e-50 | -8.615850e-47 |
| Msa0844490 | Msa1043760 | 0.801296 | 9.628754e-49 | -8.615850e-47 |
| Msa0921420 | Msa1043760 | 0.807400 | 5.150164e-50 | -8.615850e-47 |
| Msa0960390 | Msa1043760 | 0.816251 | 6.089719e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043760 | MtrunA17_Chr1g0153551 | 94.000 | 100 | 6 | 0 | 1 | 100 | 1 | 100 | 3.46e-65 | 191 |
| Msa1043760 | MtrunA17_Chr1g0153541 | 82.524 | 103 | 15 | 2 | 1 | 100 | 1 | 103 | 4.68e-55 | 166 |
| Msa1043760 | MtrunA17_Chr1g0153561 | 47.000 | 100 | 48 | 1 | 1 | 100 | 1 | 95 | 2.28e-22 | 83.6 |
| Msa1043760 | MtrunA17_Chr1g0153581 | 46.154 | 104 | 43 | 2 | 1 | 100 | 1 | 95 | 1.36e-20 | 79.0 |
| Msa1043760 | MtrunA17_Chr1g0153611 | 47.191 | 89 | 45 | 2 | 5 | 93 | 1 | 87 | 2.86e-19 | 75.5 |
| Msa1043760 | MtrunA17_Chr1g0153601 | 43.269 | 104 | 46 | 2 | 1 | 100 | 1 | 95 | 4.05e-19 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAAGCTTTCAAGGAATT+TGG | 0.161453 | 7_2:+1927657 | Msa1043760:CDS |
| CCATGACAGAAAAGCAAATA+AGG | 0.240617 | 7_2:+1927577 | Msa1043760:CDS |
| AACTCTGGCTCTCTAACTAT+AGG | 0.360916 | 7_2:-1927525 | None:intergenic |
| TGAGCTTAAGAAAGCTTTCA+AGG | 0.401327 | 7_2:+1927650 | Msa1043760:CDS |
| ACTCTGGCTCTCTAACTATA+GGG | 0.429050 | 7_2:-1927524 | None:intergenic |
| TTGGTTCGAGGATGCCTTGT+TGG | 0.440846 | 7_2:+1927676 | Msa1043760:CDS |
| AGAGCCAGAGTTCAAGTTGA+AGG | 0.480946 | 7_2:+1927536 | Msa1043760:CDS |
| CATGACAGAAAAGCAAATAA+GGG | 0.482502 | 7_2:+1927578 | Msa1043760:CDS |
| CTTTCAAGGAATTTGGTTCG+AGG | 0.492914 | 7_2:+1927664 | Msa1043760:CDS |
| AGACCCTTCAACTTGAACTC+TGG | 0.511841 | 7_2:-1927540 | None:intergenic |
| CTAACTATAGGGATGAATAC+AGG | 0.520143 | 7_2:-1927513 | None:intergenic |
| AAAGTTGATACCAATTGTGA+TGG | 0.538159 | 7_2:+1927720 | Msa1043760:CDS |
| GAGCCAGAGTTCAAGTTGAA+GGG | 0.554107 | 7_2:+1927537 | Msa1043760:CDS |
| TTGTTGATTATGTCTTAGCA+TGG | 0.561248 | 7_2:+1927772 | Msa1043760:CDS |
| TCTGTCATGGCTATGTTCTG+TGG | 0.589746 | 7_2:-1927564 | None:intergenic |
| AAAGCAGATCGAGATGGAGA+TGG | 0.597293 | 7_2:+1927612 | Msa1043760:CDS |
| TTGATCAAAGCAGATCGAGA+TGG | 0.600250 | 7_2:+1927606 | Msa1043760:CDS |
| CAACAAGAAGCTCTCCAACA+AGG | 0.621562 | 7_2:-1927690 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CATGACAGAAAAGCAAATAA+GGG | + | chr7_2:1927578-1927597 | Msa1043760:CDS | 30.0% | |
| AAGAAAGCTTTCAAGGAATT+TGG | + | chr7_2:1927657-1927676 | Msa1043760:CDS | 30.0% | |
| !! | AAAGTTGATACCAATTGTGA+TGG | + | chr7_2:1927720-1927739 | Msa1043760:CDS | 30.0% |
| !! | GCTTATTTTTCCATCACAAT+TGG | - | chr7_2:1927733-1927752 | None:intergenic | 30.0% |
| ! | ATTGTGATGGAAAAATAAGC+AGG | + | chr7_2:1927733-1927752 | Msa1043760:CDS | 30.0% |
| ! | TTGTGATGGAAAAATAAGCA+GGG | + | chr7_2:1927734-1927753 | Msa1043760:CDS | 30.0% |
| ! | TTGTTGATTATGTCTTAGCA+TGG | + | chr7_2:1927772-1927791 | Msa1043760:CDS | 30.0% |
| CTAACTATAGGGATGAATAC+AGG | - | chr7_2:1927516-1927535 | None:intergenic | 35.0% | |
| !! | CCTTATTTGCTTTTCTGTCA+TGG | - | chr7_2:1927580-1927599 | None:intergenic | 35.0% |
| CCATGACAGAAAAGCAAATA+AGG | + | chr7_2:1927577-1927596 | Msa1043760:CDS | 35.0% | |
| TGAGCTTAAGAAAGCTTTCA+AGG | + | chr7_2:1927650-1927669 | Msa1043760:CDS | 35.0% | |
| !! | ACTCTGGCTCTCTAACTATA+GGG | - | chr7_2:1927527-1927546 | None:intergenic | 40.0% |
| !! | AACTCTGGCTCTCTAACTAT+AGG | - | chr7_2:1927528-1927547 | None:intergenic | 40.0% |
| TTGATCAAAGCAGATCGAGA+TGG | + | chr7_2:1927606-1927625 | Msa1043760:CDS | 40.0% | |
| ! | CTTTCAAGGAATTTGGTTCG+AGG | + | chr7_2:1927664-1927683 | Msa1043760:CDS | 40.0% |
| !! | AGAGCCAGAGTTCAAGTTGA+AGG | + | chr7_2:1927536-1927555 | Msa1043760:CDS | 45.0% |
| !! | GAGCCAGAGTTCAAGTTGAA+GGG | + | chr7_2:1927537-1927556 | Msa1043760:CDS | 45.0% |
| AGACCCTTCAACTTGAACTC+TGG | - | chr7_2:1927543-1927562 | None:intergenic | 45.0% | |
| TCTGTCATGGCTATGTTCTG+TGG | - | chr7_2:1927567-1927586 | None:intergenic | 45.0% | |
| AAAGCAGATCGAGATGGAGA+TGG | + | chr7_2:1927612-1927631 | Msa1043760:CDS | 45.0% | |
| CAACAAGAAGCTCTCCAACA+AGG | - | chr7_2:1927693-1927712 | None:intergenic | 45.0% | |
| ! | TTGGTTCGAGGATGCCTTGT+TGG | + | chr7_2:1927676-1927695 | Msa1043760:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 1927510 | 1927812 | 1927510 | ID=Msa1043760;Name=Msa1043760 |
| chr7_2 | mRNA | 1927510 | 1927812 | 1927510 | ID=Msa1043760-mRNA-1;Parent=Msa1043760;Name=Msa1043760-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|100 |
| chr7_2 | exon | 1927510 | 1927812 | 1927510 | ID=Msa1043760-mRNA-1:exon:400;Parent=Msa1043760-mRNA-1 |
| chr7_2 | CDS | 1927510 | 1927812 | 1927510 | ID=Msa1043760-mRNA-1:cds;Parent=Msa1043760-mRNA-1 |
| Gene Sequence |
| Protein sequence |
