AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1271130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1271130 MtrunA17_Chr8g0334811 99.363 157 1 0 1 157 1 157 6.12e-110 319
Msa1271130 MtrunA17_Chr2g0322181 74.684 158 36 3 1 157 1 155 4.26e-54 176
Msa1271130 MtrunA17_Chr4g0025401 75.796 157 34 3 1 157 1 153 6.71e-53 172
Msa1271130 MtrunA17_Chr2g0333221 42.038 157 80 2 12 157 68 224 2.20e-32 120
Msa1271130 MtrunA17_Chr7g0269841 47.482 139 66 3 15 149 27 162 1.89e-30 112
Msa1271130 MtrunA17_Chr3g0123861 44.898 147 76 3 15 157 83 228 9.87e-29 109
Msa1271130 MtrunA17_Chr6g0478651 40.132 152 82 2 15 157 69 220 1.62e-28 109
Msa1271130 MtrunA17_Chr2g0282741 38.587 184 61 5 15 146 5 188 1.76e-27 104
Msa1271130 MtrunA17_Chr3g0138471 36.782 174 71 4 15 149 5 178 6.38e-27 103
Msa1271130 MtrunA17_Chr4g0040501 37.566 189 61 5 15 146 5 193 5.35e-26 100
Msa1271130 MtrunA17_Chr4g0010781 45.223 157 61 5 15 158 87 231 3.05e-25 100
Msa1271130 MtrunA17_Chr2g0282761 35.638 188 65 5 15 146 5 192 3.29e-24 96.3
Msa1271130 MtrunA17_Chr2g0324921 35.417 192 76 6 15 158 5 196 1.75e-23 94.0
Msa1271130 MtrunA17_Chr4g0006391 35.417 192 72 6 15 154 5 196 4.73e-23 93.2
Msa1271130 MtrunA17_Chr3g0087741 35.256 156 76 5 15 158 90 232 4.44e-19 82.8
Msa1271130 MtrunA17_Chr3g0093111 31.098 164 81 6 15 149 3 163 2.52e-18 79.7
Msa1271130 MtrunA17_Chr8g0352451 47.959 98 40 3 13 106 71 161 1.59e-17 78.2
Msa1271130 MtrunA17_Chr5g0399201 44.048 84 40 1 11 87 9 92 7.85e-17 76.6
Msa1271130 MtrunA17_Chr5g0393371 43.750 96 43 3 13 104 5 93 1.30e-16 75.9
Msa1271130 MtrunA17_Chr1g0159511 43.750 96 43 3 13 104 5 93 1.52e-16 75.5
Msa1271130 MtrunA17_Chr1g0159491 49.296 71 32 2 13 79 5 75 2.07e-16 69.7
Msa1271130 MtrunA17_Chr1g0209551 52.941 68 28 1 16 79 18 85 2.21e-15 72.4
Msa1271130 MtrunA17_Chr7g0263971 47.561 82 37 2 1 77 356 436 3.81e-14 68.9
Msa1271130 MtrunA17_Chr7g0242951 49.231 65 30 1 16 77 29 93 2.04e-13 66.6
Msa1271130 MtrunA17_Chr2g0307061 48.529 68 32 1 15 79 111 178 2.44e-13 66.2
Msa1271130 MtrunA17_Chr1g0206121 51.667 60 26 1 16 72 37 96 8.21e-13 64.7
Msa1271130 MtrunA17_Chr2g0295821 38.462 117 51 5 13 108 103 219 1.15e-12 64.3
Msa1271130 MtrunA17_Chr5g0414471 46.377 69 33 1 16 80 27 95 1.22e-12 64.3
Msa1271130 MtrunA17_Chr5g0415731 28.188 149 70 3 12 148 3 126 1.74e-12 61.2
Msa1271130 MtrunA17_Chr4g0061421 32.692 104 62 1 15 110 9 112 2.15e-12 63.9
Msa1271130 MtrunA17_Chr2g0301881 40.000 75 41 1 16 86 101 175 3.72e-12 63.2
Msa1271130 MtrunA17_Chr7g0271891 41.333 75 36 1 15 81 17 91 4.88e-12 60.5
Msa1271130 MtrunA17_Chr7g0258061 51.562 64 27 2 16 75 24 87 1.13e-11 61.2
Msa1271130 MtrunA17_Chr3g0091361 45.333 75 36 2 14 83 5 79 1.23e-11 60.5
Msa1271130 MtrunA17_Chr1g0173931 50.000 64 28 2 16 75 40 103 3.56e-11 59.7
Msa1271130 MtrunA17_Chr8g0371891 41.096 73 37 1 7 73 40 112 3.87e-11 57.8
Msa1271130 MtrunA17_Chr5g0405521 44.615 65 30 1 15 73 73 137 4.22e-11 58.2
Msa1271130 MtrunA17_Chr5g0426951 29.032 155 92 5 15 157 84 232 6.18e-11 59.7
Msa1271130 MtrunA17_Chr4g0021141 43.077 65 33 1 15 75 23 87 9.58e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1271130 AT5G22060.1 75.316 158 36 2 1 157 1 156 2.57e-68 213
Msa1271130 AT3G44110.2 77.070 157 34 1 1 157 1 155 2.21e-64 201
Msa1271130 AT3G44110.1 77.070 157 34 1 1 157 1 155 6.53e-64 202
Msa1271130 AT1G80030.4 46.104 154 72 2 15 157 76 229 2.70e-36 130
Msa1271130 AT1G80030.2 46.104 154 72 2 15 157 76 229 3.02e-36 130
Msa1271130 AT1G80030.3 46.104 154 72 2 15 157 76 229 3.02e-36 130
Msa1271130 AT1G80030.1 46.104 154 72 2 15 157 76 229 3.02e-36 130
Msa1271130 AT2G22360.1 47.260 146 73 2 15 157 87 231 4.93e-31 116
Msa1271130 AT5G25530.2 37.634 186 65 6 15 149 5 190 1.42e-27 105
Msa1271130 AT3G62600.1 45.070 142 67 4 15 149 27 164 2.32e-27 105
Msa1271130 AT5G01390.4 38.587 184 66 7 15 151 5 188 5.71e-27 101
Msa1271130 AT4G39960.1 41.667 156 80 3 12 157 83 237 8.68e-27 104
Msa1271130 AT4G39960.2 41.667 156 80 3 12 157 83 237 8.68e-27 104
Msa1271130 AT5G01390.1 38.587 184 66 7 15 151 5 188 2.64e-26 102
Msa1271130 AT5G48030.1 40.127 157 76 4 15 158 95 246 6.96e-25 99.8
Msa1271130 AT5G25530.1 36.082 194 65 7 15 149 5 198 9.20e-25 98.2
Msa1271130 AT2G20560.1 37.363 182 64 5 15 146 5 186 2.01e-24 97.1
Msa1271130 AT3G08910.1 36.257 171 75 5 15 151 5 175 3.20e-24 96.3
Msa1271130 AT5G01390.3 43.066 137 51 6 15 124 5 141 4.51e-23 91.7
Msa1271130 AT5G01390.2 43.066 137 51 6 15 124 5 141 1.05e-22 91.3
Msa1271130 AT1G59725.1 32.000 175 75 4 15 146 5 178 1.59e-20 86.3
Msa1271130 AT4G28480.1 32.653 196 71 6 15 149 5 200 1.09e-19 84.3
Msa1271130 AT4G28480.2 54.930 71 27 1 15 80 5 75 2.77e-19 82.4
Msa1271130 AT3G47940.1 31.443 194 73 6 15 148 5 198 3.70e-19 82.8
Msa1271130 AT1G10350.1 29.016 193 76 6 15 146 5 197 4.03e-18 80.1
Msa1271130 AT1G77020.1 49.485 97 36 4 13 104 5 93 5.59e-18 79.7
Msa1271130 AT2G21510.5 44.330 97 48 3 13 103 5 101 2.70e-17 77.4
Msa1271130 AT2G21510.2 44.330 97 48 3 13 103 5 101 3.01e-17 77.8
Msa1271130 AT2G21510.4 44.330 97 48 3 13 103 5 101 3.01e-17 77.8
Msa1271130 AT2G21510.3 44.330 97 48 3 13 103 5 101 3.18e-17 77.4
Msa1271130 AT2G21510.1 44.330 97 48 3 13 103 5 101 3.18e-17 77.4
Msa1271130 AT3G17830.1 33.544 158 92 3 13 157 62 219 1.22e-16 76.6
Msa1271130 AT4G39150.3 44.330 97 48 3 13 103 5 101 3.01e-16 74.7
Msa1271130 AT4G39150.1 44.330 97 48 3 13 103 5 101 3.01e-16 74.7
Msa1271130 AT4G39150.2 44.330 97 48 3 13 103 5 101 3.01e-16 74.7
Msa1271130 AT1G21080.3 42.708 96 43 3 13 104 5 92 1.58e-15 73.2
Msa1271130 AT1G21080.1 42.708 96 43 3 13 104 5 92 1.82e-15 72.8
Msa1271130 AT1G59980.2 48.529 68 31 1 16 79 25 92 4.10e-15 71.6
Msa1271130 AT1G59980.1 48.529 68 31 1 16 79 25 92 6.91e-15 71.2
Msa1271130 AT1G28210.2 32.680 153 85 6 15 157 49 193 8.01e-15 71.2
Msa1271130 AT1G28210.1 32.680 153 85 6 15 157 49 193 8.62e-15 70.9
Msa1271130 AT1G28210.3 32.680 153 85 6 15 157 49 193 8.81e-15 70.9
Msa1271130 AT1G76700.1 41.584 101 48 3 13 109 5 98 1.53e-14 70.1
Msa1271130 AT3G08970.1 48.649 74 35 1 16 86 29 102 2.51e-14 69.7
Msa1271130 AT3G08970.2 48.649 74 35 1 16 86 29 102 2.51e-14 69.7
Msa1271130 AT1G68370.1 51.471 68 29 1 16 79 19 86 4.59e-14 68.9
Msa1271130 AT2G35720.1 41.772 79 39 1 12 83 13 91 2.80e-13 66.6
Msa1271130 AT4G13830.3 39.189 74 39 1 6 73 58 131 4.71e-13 63.9
Msa1271130 AT4G13830.1 39.189 74 39 1 6 73 58 131 5.88e-13 63.2
Msa1271130 AT4G21180.1 38.095 84 48 1 7 86 92 175 7.92e-13 65.5
Msa1271130 AT1G24120.1 46.377 69 33 2 16 80 22 90 8.60e-13 65.5
Msa1271130 AT5G06910.1 37.963 108 52 4 1 102 19 117 1.11e-12 64.3
Msa1271130 AT4G21180.2 38.095 84 48 1 7 86 12 95 1.49e-12 64.7
Msa1271130 AT4G13830.2 39.189 74 39 1 6 73 58 131 1.84e-12 62.8
Msa1271130 AT5G49060.2 47.761 67 32 1 12 75 97 163 2.16e-12 63.9
Msa1271130 AT5G49060.1 47.761 67 32 1 12 75 97 163 2.16e-12 63.9
Msa1271130 AT5G03160.1 50.000 86 34 5 15 95 371 452 2.28e-12 64.3
Msa1271130 AT1G56300.2 42.647 68 31 1 13 72 12 79 3.61e-12 60.1
Msa1271130 AT1G56300.1 42.647 68 31 1 13 72 12 79 6.60e-12 60.5
Msa1271130 AT1G74250.1 41.250 80 38 2 9 79 6 85 7.72e-12 62.8
Msa1271130 AT5G12430.1 41.111 90 29 2 7 72 1017 1106 8.53e-12 62.8
Msa1271130 AT3G12170.2 43.662 71 36 1 9 75 42 112 1.05e-11 61.6
Msa1271130 AT4G10130.2 43.939 66 30 1 15 73 12 77 1.14e-11 60.1
Msa1271130 AT4G10130.4 43.939 66 30 1 15 73 12 77 1.14e-11 60.1
Msa1271130 AT4G10130.1 43.939 66 30 1 15 73 12 77 1.14e-11 60.1
Msa1271130 AT4G10130.3 43.939 66 30 1 15 73 12 77 1.14e-11 60.1
Msa1271130 AT4G10130.5 43.939 66 30 1 15 73 12 77 1.14e-11 60.1
Msa1271130 AT3G12170.1 43.662 71 36 1 9 75 6 76 1.32e-11 61.2
Msa1271130 AT5G05750.1 31.469 143 69 5 15 132 115 253 2.91e-11 60.5
Msa1271130 AT3G57340.1 43.939 66 34 1 15 77 114 179 4.31e-11 60.5
Msa1271130 AT3G57340.2 43.939 66 34 1 15 77 114 179 4.31e-11 60.5
Msa1271130 AT5G16650.2 47.059 68 32 1 9 72 6 73 6.70e-11 58.2
Msa1271130 AT5G16650.1 50.000 68 30 2 9 72 6 73 9.02e-11 57.0

Find 47 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTAGTTATCACAACTTAA+AGG 0.233868 8_3:+4568060 Msa1271130:five_prime_UTR
ACCAAGAATATCGTAATATT+TGG 0.253782 8_3:-4569268 None:intergenic
ACCAAATATTACGATATTCT+TGG 0.278321 8_3:+4569267 Msa1271130:CDS
CAAAGAAGCTATCACTTTCT+AGG 0.280955 8_3:+4570522 Msa1271130:CDS
TCAGGATCCCCACCCTTATC+TGG 0.281419 8_3:-4569357 None:intergenic
CTTCATCGCTGCCTTTCTAT+AGG 0.293338 8_3:-4569328 None:intergenic
TAAGGTGCATCAATCATGTT+TGG 0.331511 8_3:+4569216 Msa1271130:exon
GTGTTATCACTCTTCCTTGT+TGG 0.332109 8_3:-4569246 None:intergenic
AGATGTGGTGCATTCTATAA+AGG 0.359454 8_3:+4570469 Msa1271130:CDS
TTTAGTTATCACAACTTAAA+GGG 0.375522 8_3:+4568061 Msa1271130:five_prime_UTR
AAAGAACTGTATGATCAATA+TGG 0.384904 8_3:+4570206 Msa1271130:CDS
TCTATATCAGTTCAAGGAGT+TGG 0.404313 8_3:+4570148 Msa1271130:intron
CTATATCAGTTCAAGGAGTT+GGG 0.407932 8_3:+4570149 Msa1271130:intron
CACTGTGCTCAAAATGTAAA+GGG 0.423759 8_3:+4570549 Msa1271130:CDS
GCATTCTATAAAGGTTTCCT+TGG 0.436569 8_3:+4570478 Msa1271130:CDS
TTCTATAAAGGTTTCCTTGG+AGG 0.438895 8_3:+4570481 Msa1271130:CDS
CGCTCAACACTTCATATGCT+TGG 0.448770 8_3:-4570172 None:intergenic
TTCTGTCTTCTTGCGCGTGA+AGG 0.455820 8_3:-4570437 None:intergenic
AAGGTGCATCAATCATGTTT+GGG 0.456450 8_3:+4569217 Msa1271130:exon
ACCATGAGGCTAACCTTCTC+AGG 0.464942 8_3:-4569375 None:intergenic
TCCTTGGAGGATGTGTATAA+CGG 0.490673 8_3:+4570494 Msa1271130:CDS
ATGAAGAACCATCCAGATAA+GGG 0.493298 8_3:+4569345 Msa1271130:CDS
GCACTGTGCTCAAAATGTAA+AGG 0.502752 8_3:+4570548 Msa1271130:CDS
GGTGAAGATGCCCTTAAAGA+AGG 0.514253 8_3:+4570227 Msa1271130:CDS
GATGCCCTTAAAGAAGGAAT+GGG 0.517795 8_3:+4570233 Msa1271130:CDS
GATGAAGAACCATCCAGATA+AGG 0.518235 8_3:+4569344 Msa1271130:CDS
AGATGCCCTTAAAGAAGGAA+TGG 0.543006 8_3:+4570232 Msa1271130:CDS
ACCAACGTGCGAAATTCTGT+TGG 0.544347 8_3:+4568215 Msa1271130:five_prime_UTR
AATGATTCGAAAATATCAAA+CGG 0.546049 8_3:-4570284 None:intergenic
AATCAAGAAAGCCTATAGAA+AGG 0.546291 8_3:+4569317 Msa1271130:CDS
TGCTCAAAATGTAAAGGGTA+CGG 0.546774 8_3:+4570554 Msa1271130:CDS
GATCCCCACCCTTATCTGGA+TGG 0.550815 8_3:-4569353 None:intergenic
CCAACGTGCGAAATTCTGTT+GGG 0.555543 8_3:+4568216 Msa1271130:five_prime_UTR
ACAGAAGCAAGGAGAAGATG+TGG 0.556990 8_3:+4570454 Msa1271130:CDS
GCATCAATCATGTTTGGGCG+CGG 0.585791 8_3:+4569222 Msa1271130:exon
AGAACCATCCAGATAAGGGT+GGG 0.600127 8_3:+4569349 Msa1271130:CDS
TAAGGGTGGGGATCCTGAGA+AGG 0.603050 8_3:+4569362 Msa1271130:CDS
GAACCATCCAGATAAGGGTG+GGG 0.607182 8_3:+4569350 Msa1271130:CDS
GCCGTTATACACATCCTCCA+AGG 0.620522 8_3:-4570495 None:intergenic
GAGTACAGAACATTTAAGCA+TGG 0.627821 8_3:-4570583 None:intergenic
CGCGCAAGAAGACAGAAGCA+AGG 0.629132 8_3:+4570443 Msa1271130:CDS
CCCAACAGAATTTCGCACGT+TGG 0.636763 8_3:-4568216 None:intergenic
AAGAACCATCCAGATAAGGG+TGG 0.639918 8_3:+4569348 Msa1271130:CDS
ATGCCCTTAAAGAAGGAATG+GGG 0.645770 8_3:+4570234 Msa1271130:CDS
TGCCCTTAAAGAAGGAATGG+GGG 0.646167 8_3:+4570235 Msa1271130:CDS
GCCCTTAAAGAAGGAATGGG+GGG 0.649818 8_3:+4570236 Msa1271130:CDS
TGTTTGGGCGCGGACCAACA+AGG 0.669824 8_3:+4569232 Msa1271130:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCGATTTTATATTGAATTTT+AGG + chr8_3:4568449-4568468 Msa1271130:intron 15.0%
!!! GAAAATAAAATTACAACTTT+TGG - chr8_3:4568503-4568522 None:intergenic 15.0%
!!! AAAGTTGTAATTTTATTTTC+TGG + chr8_3:4568503-4568522 Msa1271130:intron 15.0%
!!! AAGTTGTAATTTTATTTTCT+GGG + chr8_3:4568504-4568523 Msa1271130:intron 15.0%
!!! AATTCACATTATAAAGTTTT+TGG + chr8_3:4569098-4569117 Msa1271130:intron 15.0%
!! TGATATAGAAACAATATATT+AGG - chr8_3:4570137-4570156 None:intergenic 15.0%
!! TTTAGTTATCACAACTTAAA+GGG + chr8_3:4568061-4568080 Msa1271130:five_prime_UTR 20.0%
!! AGAAATCAAATAGAAAGAAT+AGG - chr8_3:4568258-4568277 None:intergenic 20.0%
!!! ATTCTTTCTATTTGATTTCT+AGG + chr8_3:4568258-4568277 Msa1271130:intron 20.0%
!!! TTCTTTCTATTTGATTTCTA+GGG + chr8_3:4568259-4568278 Msa1271130:intron 20.0%
!! TTGAATCGATGATAAAATTT+GGG - chr8_3:4568349-4568368 None:intergenic 20.0%
!! ATTGAATCGATGATAAAATT+TGG - chr8_3:4568350-4568369 None:intergenic 20.0%
!!! ATTTTATCATCGATTCAATT+AGG + chr8_3:4568351-4568370 Msa1271130:intron 20.0%
!!! TTTTATCATCGATTCAATTA+GGG + chr8_3:4568352-4568371 Msa1271130:intron 20.0%
!!! TTAAAGTTTCAAAGTTTTGT+TGG + chr8_3:4568411-4568430 Msa1271130:intron 20.0%
!!! TAAAGTTTCAAAGTTTTGTT+GGG + chr8_3:4568412-4568431 Msa1271130:intron 20.0%
!!! AGTTGTAATTTTATTTTCTG+GGG + chr8_3:4568505-4568524 Msa1271130:intron 20.0%
!!! AGATTTTGTTGTATGAAAAT+TGG + chr8_3:4568632-4568651 Msa1271130:intron 20.0%
!!! AAAATAGTTTTATGGACAAT+TGG + chr8_3:4569568-4569587 Msa1271130:intron 20.0%
!!! TCTCCTAAAAATATTGTTTT+AGG + chr8_3:4569886-4569905 Msa1271130:intron 20.0%
!!! CTCCTAAAAATATTGTTTTA+GGG + chr8_3:4569887-4569906 Msa1271130:intron 20.0%
!!! TCCTAAAAATATTGTTTTAG+GGG + chr8_3:4569888-4569907 Msa1271130:intron 20.0%
!! AATGATTCGAAAATATCAAA+CGG - chr8_3:4570287-4570306 None:intergenic 20.0%
!!! GATATTTTCGAATCATTTTT+TGG + chr8_3:4570290-4570309 Msa1271130:CDS 20.0%
! GTTTAGTTATCACAACTTAA+AGG + chr8_3:4568060-4568079 Msa1271130:five_prime_UTR 25.0%
!! ATGATAAAATTTGGGATCTA+AGG - chr8_3:4568341-4568360 None:intergenic 25.0%
!!! AGTCTTGTTTATAGTTATGA+TGG + chr8_3:4568590-4568609 Msa1271130:intron 25.0%
!!! CATTATAAAGTTTTTGGTTG+TGG + chr8_3:4569104-4569123 Msa1271130:intron 25.0%
!!! GTTTTTGTGTAGTGTTTTTA+AGG + chr8_3:4569198-4569217 Msa1271130:intron 25.0%
! ACCAAATATTACGATATTCT+TGG + chr8_3:4569267-4569286 Msa1271130:CDS 25.0%
! ACCAAGAATATCGTAATATT+TGG - chr8_3:4569271-4569290 None:intergenic 25.0%
! ATTTAAGTGAGACACAAATT+TGG - chr8_3:4569674-4569693 None:intergenic 25.0%
!! ACCCCTAAAACAATATTTTT+AGG - chr8_3:4569892-4569911 None:intergenic 25.0%
!!! AAAAATATTGTTTTAGGGGT+AGG + chr8_3:4569892-4569911 Msa1271130:intron 25.0%
!!! ATTGTTTCTATATCAGTTCA+AGG + chr8_3:4570142-4570161 Msa1271130:intron 25.0%
! AAAGAACTGTATGATCAATA+TGG + chr8_3:4570206-4570225 Msa1271130:CDS 25.0%
TTCTTCTTCTTCTTCTTCAA+GGG - chr8_3:4568185-4568204 None:intergenic 30.0%
AGATCTGTTTCGATTCAATT+AGG + chr8_3:4568285-4568304 Msa1271130:intron 30.0%
GATCTGTTTCGATTCAATTA+GGG + chr8_3:4568286-4568305 Msa1271130:intron 30.0%
!!! GTAATTTTATTTTCTGGGGA+AGG + chr8_3:4568509-4568528 Msa1271130:intron 30.0%
! TGATGGTATTGAAATTAGCT+TGG + chr8_3:4568607-4568626 Msa1271130:intron 30.0%
! GATGGTATTGAAATTAGCTT+GGG + chr8_3:4568608-4568627 Msa1271130:intron 30.0%
TGAATGTAATCACATGTTGA+CGG + chr8_3:4568730-4568749 Msa1271130:intron 30.0%
! AGATGGTATTGAATTTAACC+TGG + chr8_3:4568871-4568890 Msa1271130:intron 30.0%
! GATGGTATTGAATTTAACCT+GGG + chr8_3:4568872-4568891 Msa1271130:intron 30.0%
!!! TTTAACCTGGGTAGATTTTT+TGG + chr8_3:4568884-4568903 Msa1271130:intron 30.0%
ATCATGCACAATATACTACA+TGG - chr8_3:4568924-4568943 None:intergenic 30.0%
! GTTAGTTTCAATGCATGAAA+TGG + chr8_3:4568967-4568986 Msa1271130:intron 30.0%
AATGCATGAAATGGAGTTTA+TGG + chr8_3:4568976-4568995 Msa1271130:intron 30.0%
AACACTACACAAAAACAAGA+AGG - chr8_3:4569194-4569213 None:intergenic 30.0%
AATCAAGAAAGCCTATAGAA+AGG + chr8_3:4569317-4569336 Msa1271130:CDS 30.0%
AACTTTATTAGCAGAGATAG+AGG - chr8_3:4569528-4569547 None:intergenic 30.0%
!! CCATCATTTTTGGACATTTT+GGG + chr8_3:4569732-4569751 Msa1271130:intron 30.0%
!!! TTTTTGGTTGTACGAAAAGA+GGG - chr8_3:4569794-4569813 None:intergenic 30.0%
AAGCAAAAGTTGCAGTATTA+GGG - chr8_3:4569833-4569852 None:intergenic 30.0%
TAAGCAAAAGTTGCAGTATT+AGG - chr8_3:4569834-4569853 None:intergenic 30.0%
!! TTTTTAGGAGAAGTTGAAGT+CGG - chr8_3:4569877-4569896 None:intergenic 30.0%
GGGATAATTCAATATTACTG+TGG + chr8_3:4569953-4569972 Msa1271130:intron 30.0%
GGATTGAAACAACAAAATCA+AGG - chr8_3:4570116-4570135 None:intergenic 30.0%
! TCATACAGTTCTTTCTTTTC+AGG - chr8_3:4570200-4570219 None:intergenic 30.0%
!! CACTATTTCTATTTGCTCTT+AGG + chr8_3:4570407-4570426 Msa1271130:intron 30.0%
! TATTTCTATTTGCTCTTAGG+TGG + chr8_3:4570410-4570429 Msa1271130:intron 30.0%
GTTCTTCTTCTTCTTCTTCA+AGG - chr8_3:4568186-4568205 None:intergenic 35.0%
! AATTTGGGATCTAAGGAACA+CGG - chr8_3:4568334-4568353 None:intergenic 35.0%
!! GTTTTGTTGGGTTTCTGTTA+AGG + chr8_3:4568424-4568443 Msa1271130:intron 35.0%
ATATTGTGCTTCATTTGAGC+TGG + chr8_3:4568667-4568686 Msa1271130:intron 35.0%
TTCATCCAAAAAATCTACCC+AGG - chr8_3:4568892-4568911 None:intergenic 35.0%
ATATTGTGCATGATTTGAGC+TGG + chr8_3:4568930-4568949 Msa1271130:intron 35.0%
!! GAGTGTTGTTCTTTTTGTGT+AGG + chr8_3:4569004-4569023 Msa1271130:intron 35.0%
TAAGGTGCATCAATCATGTT+TGG + chr8_3:4569216-4569235 Msa1271130:exon 35.0%
AAGGTGCATCAATCATGTTT+GGG + chr8_3:4569217-4569236 Msa1271130:exon 35.0%
ATGAAGAACCATCCAGATAA+GGG + chr8_3:4569345-4569364 Msa1271130:CDS 35.0%
ATGTGAATACCCTGCTATTA+CGG + chr8_3:4569439-4569458 Msa1271130:intron 35.0%
AGTCAGAATGCTAACAAACA+AGG - chr8_3:4569466-4569485 None:intergenic 35.0%
!!! TATAAGTGGAGGGCATTTTT+CGG - chr8_3:4569711-4569730 None:intergenic 35.0%
AAATGATGGCTATAAGTGGA+GGG - chr8_3:4569721-4569740 None:intergenic 35.0%
AAAATGATGGCTATAAGTGG+AGG - chr8_3:4569722-4569741 None:intergenic 35.0%
CCAAAAATGATGGCTATAAG+TGG - chr8_3:4569725-4569744 None:intergenic 35.0%
! CCACTTATAGCCATCATTTT+TGG + chr8_3:4569722-4569741 Msa1271130:intron 35.0%
!! GCCATCATTTTTGGACATTT+TGG + chr8_3:4569731-4569750 Msa1271130:intron 35.0%
CCCAAAATGTCCAAAAATGA+TGG - chr8_3:4569735-4569754 None:intergenic 35.0%
! TTATTCACCCGAGTCTTTTA+GGG - chr8_3:4569761-4569780 None:intergenic 35.0%
!!! GTTTTTGGTTGTACGAAAAG+AGG - chr8_3:4569795-4569814 None:intergenic 35.0%
!!! AATATTGTTTTAGGGGTAGG+CGG + chr8_3:4569895-4569914 Msa1271130:intron 35.0%
TGTTCTTATGCATACTTCTG+TGG + chr8_3:4570056-4570075 Msa1271130:intron 35.0%
AAATCAAGGTCAGCAACAAA+AGG - chr8_3:4570102-4570121 None:intergenic 35.0%
TCTATATCAGTTCAAGGAGT+TGG + chr8_3:4570148-4570167 Msa1271130:intron 35.0%
CTATATCAGTTCAAGGAGTT+GGG + chr8_3:4570149-4570168 Msa1271130:intron 35.0%
!!! TTCGAATCATTTTTTGGTGC+AGG + chr8_3:4570296-4570315 Msa1271130:CDS 35.0%
AGATGTGGTGCATTCTATAA+AGG + chr8_3:4570469-4570488 Msa1271130:CDS 35.0%
GCATTCTATAAAGGTTTCCT+TGG + chr8_3:4570478-4570497 Msa1271130:CDS 35.0%
TTCTATAAAGGTTTCCTTGG+AGG + chr8_3:4570481-4570500 Msa1271130:CDS 35.0%
CAAAGAAGCTATCACTTTCT+AGG + chr8_3:4570522-4570541 Msa1271130:CDS 35.0%
CACTGTGCTCAAAATGTAAA+GGG + chr8_3:4570549-4570568 Msa1271130:CDS 35.0%
TGCTCAAAATGTAAAGGGTA+CGG + chr8_3:4570554-4570573 Msa1271130:CDS 35.0%
! TCTCTCTTTTTCTCTCTTCG+AGG + chr8_3:4568146-4568165 Msa1271130:five_prime_UTR 40.0%
! CTCTCTTTTTCTCTCTTCGA+GGG + chr8_3:4568147-4568166 Msa1271130:five_prime_UTR 40.0%
ATGTAATCACATGTTGACGG+TGG + chr8_3:4568733-4568752 Msa1271130:intron 40.0%
TAGTGAAGGAGAGTTTGTGA+GGG + chr8_3:4568779-4568798 Msa1271130:intron 40.0%
TTGTAGCAGTAGGTATCAGA+TGG + chr8_3:4568854-4568873 Msa1271130:intron 40.0%
TGAAATGGAGTTTATGGTCG+TGG + chr8_3:4568982-4569001 Msa1271130:intron 40.0%
GTGTTATCACTCTTCCTTGT+TGG - chr8_3:4569249-4569268 None:intergenic 40.0%
GATGAAGAACCATCCAGATA+AGG + chr8_3:4569344-4569363 Msa1271130:CDS 40.0%
ACAAAGTAGCAGTAACCATG+AGG - chr8_3:4569392-4569411 None:intergenic 40.0%
AAACAAGGACCGTAATAGCA+GGG - chr8_3:4569451-4569470 None:intergenic 40.0%
TTGGGTATCCCTAAAAGACT+CGG + chr8_3:4569750-4569769 Msa1271130:intron 40.0%
! GTTATTCACCCGAGTCTTTT+AGG - chr8_3:4569762-4569781 None:intergenic 40.0%
! CTTTCAGACACATGGGTTTT+TGG - chr8_3:4569810-4569829 None:intergenic 40.0%
ATTAGGGCTTTCAGACACAT+GGG - chr8_3:4569817-4569836 None:intergenic 40.0%
TATTAGGGCTTTCAGACACA+TGG - chr8_3:4569818-4569837 None:intergenic 40.0%
TTGCTTATCCTTCTCTTGTG+CGG + chr8_3:4569847-4569866 Msa1271130:intron 40.0%
ATATTACTGTGGCATGCAGA+TGG + chr8_3:4569964-4569983 Msa1271130:intron 40.0%
AGATGCCCTTAAAGAAGGAA+TGG + chr8_3:4570232-4570251 Msa1271130:CDS 40.0%
GATGCCCTTAAAGAAGGAAT+GGG + chr8_3:4570233-4570252 Msa1271130:CDS 40.0%
ATGCCCTTAAAGAAGGAATG+GGG + chr8_3:4570234-4570253 Msa1271130:CDS 40.0%
!!! TCATTTTTTGGTGCAGGCTT+TGG + chr8_3:4570302-4570321 Msa1271130:CDS 40.0%
TTCTATTTGCTCTTAGGTGG+TGG + chr8_3:4570413-4570432 Msa1271130:intron 40.0%
TCCTTGGAGGATGTGTATAA+CGG + chr8_3:4570494-4570513 Msa1271130:CDS 40.0%
GCACTGTGCTCAAAATGTAA+AGG + chr8_3:4570548-4570567 Msa1271130:CDS 40.0%
ACCAACGTGCGAAATTCTGT+TGG + chr8_3:4568215-4568234 Msa1271130:five_prime_UTR 45.0%
CCAACGTGCGAAATTCTGTT+GGG + chr8_3:4568216-4568235 Msa1271130:five_prime_UTR 45.0%
CTTCAAGTGGGTAGTAGTGA+AGG + chr8_3:4568765-4568784 Msa1271130:intron 45.0%
GTAGTGAAGGAGAGTTTGTG+AGG + chr8_3:4568778-4568797 Msa1271130:intron 45.0%
AGTGAAGGAGAGTTTGTGAG+GGG + chr8_3:4568780-4568799 Msa1271130:intron 45.0%
GAGGAAGAGATGATGTGGAA+GGG + chr8_3:4568803-4568822 Msa1271130:intron 45.0%
AGGAAGAGATGATGTGGAAG+GGG + chr8_3:4568804-4568823 Msa1271130:intron 45.0%
! GTGGTTGTTGTTGTAGCAGT+AGG + chr8_3:4568844-4568863 Msa1271130:intron 45.0%
GAGATGCTCTGTGCTTGATT+CGG + chr8_3:4569072-4569091 Msa1271130:intron 45.0%
CTTCATCGCTGCCTTTCTAT+AGG - chr8_3:4569331-4569350 None:intergenic 45.0%
AAGAACCATCCAGATAAGGG+TGG + chr8_3:4569348-4569367 Msa1271130:CDS 45.0%
AGAACCATCCAGATAAGGGT+GGG + chr8_3:4569349-4569368 Msa1271130:CDS 45.0%
CAAACAAGGACCGTAATAGC+AGG - chr8_3:4569452-4569471 None:intergenic 45.0%
ATTCGTAGCGCTCACTATTG+TGG + chr8_3:4569648-4569667 Msa1271130:intron 45.0%
TGGGTATCCCTAAAAGACTC+GGG + chr8_3:4569751-4569770 Msa1271130:intron 45.0%
TACGAAAAGAGGGAAGCTGT+AGG - chr8_3:4569784-4569803 None:intergenic 45.0%
TAGGAGAAGTTGAAGTCGGA+AGG - chr8_3:4569873-4569892 None:intergenic 45.0%
ATTATCCCTCAAGTAGCCAG+TGG - chr8_3:4569941-4569960 None:intergenic 45.0%
CGCTCAACACTTCATATGCT+TGG - chr8_3:4570175-4570194 None:intergenic 45.0%
GGTGAAGATGCCCTTAAAGA+AGG + chr8_3:4570227-4570246 Msa1271130:CDS 45.0%
TGCCCTTAAAGAAGGAATGG+GGG + chr8_3:4570235-4570254 Msa1271130:CDS 45.0%
TCCCCCCATTCCTTCTTTAA+GGG - chr8_3:4570240-4570259 None:intergenic 45.0%
!!! TTTTTTGGTGCAGGCTTTGG+TGG + chr8_3:4570305-4570324 Msa1271130:CDS 45.0%
ACAGAAGCAAGGAGAAGATG+TGG + chr8_3:4570454-4570473 Msa1271130:CDS 45.0%
!! AAAAAAAAAAAAAAAAACTT+CGG - chr8_3:4568561-4568580 None:intergenic 5.0%
!! AAATAAAATACAAAAAATTA+AGG - chr8_3:4569157-4569176 None:intergenic 5.0%
!! TAATTATACTTAATATAAAT+TGG + chr8_3:4569494-4569513 Msa1271130:intron 5.0%
!!! AAATAAATAAAATAGTTTTA+TGG + chr8_3:4569560-4569579 Msa1271130:intron 5.0%
CCCAACAGAATTTCGCACGT+TGG - chr8_3:4568219-4568238 None:intergenic 50.0%
TGGTGAAGCGTGCTTCAAGT+GGG + chr8_3:4568753-4568772 Msa1271130:intron 50.0%
GTGAAGGAGAGTTTGTGAGG+GGG + chr8_3:4568781-4568800 Msa1271130:intron 50.0%
GGAGGAAGAGATGATGTGGA+AGG + chr8_3:4568802-4568821 Msa1271130:intron 50.0%
AGATGATGTGGAAGGGGTTG+TGG + chr8_3:4568810-4568829 Msa1271130:intron 50.0%
GCATCAATCATGTTTGGGCG+CGG + chr8_3:4569222-4569241 Msa1271130:exon 50.0%
GAACCATCCAGATAAGGGTG+GGG + chr8_3:4569350-4569369 Msa1271130:CDS 50.0%
!! TCCTGAGAAGGTTAGCCTCA+TGG + chr8_3:4569374-4569393 Msa1271130:intron 50.0%
ACCATGAGGCTAACCTTCTC+AGG - chr8_3:4569378-4569397 None:intergenic 50.0%
TGGACAATTGGCAGCCATAG+TGG + chr8_3:4569580-4569599 Msa1271130:intron 50.0%
CTGCAACTGCTCTTGTGTAC+TGG + chr8_3:4569621-4569640 Msa1271130:intron 50.0%
GCCCTTAAAGAAGGAATGGG+GGG + chr8_3:4570236-4570255 Msa1271130:CDS 50.0%
CTCCCCCCATTCCTTCTTTA+AGG - chr8_3:4570241-4570260 None:intergenic 50.0%
CTTAAAGAAGGAATGGGGGG+AGG + chr8_3:4570239-4570258 Msa1271130:CDS 50.0%
TTCTGTCTTCTTGCGCGTGA+AGG - chr8_3:4570440-4570459 None:intergenic 50.0%
GCCGTTATACACATCCTCCA+AGG - chr8_3:4570498-4570517 None:intergenic 50.0%
GTGGTGAAGCGTGCTTCAAG+TGG + chr8_3:4568752-4568771 Msa1271130:intron 55.0%
AAGGAGAGTTTGTGAGGGGG+AGG + chr8_3:4568784-4568803 Msa1271130:intron 55.0%
AGGGGGAGGAAGAGATGATG+TGG + chr8_3:4568798-4568817 Msa1271130:intron 55.0%
TGATGTGGAAGGGGTTGTGG+TGG + chr8_3:4568813-4568832 Msa1271130:intron 55.0%
GATGTGGAAGGGGTTGTGGT+GGG + chr8_3:4568814-4568833 Msa1271130:intron 55.0%
GATCCCCACCCTTATCTGGA+TGG - chr8_3:4569356-4569375 None:intergenic 55.0%
TCAGGATCCCCACCCTTATC+TGG - chr8_3:4569360-4569379 None:intergenic 55.0%
TAAGGGTGGGGATCCTGAGA+AGG + chr8_3:4569362-4569381 Msa1271130:CDS 55.0%
ATTGGCAGCCATAGTGGCTG+CGG + chr8_3:4569586-4569605 Msa1271130:intron 55.0%
ATCCCTCAAGTAGCCAGTGG+CGG - chr8_3:4569938-4569957 None:intergenic 55.0%
CGCGCAAGAAGACAGAAGCA+AGG + chr8_3:4570443-4570462 Msa1271130:CDS 55.0%
TGTTTGGGCGCGGACCAACA+AGG + chr8_3:4569232-4569251 Msa1271130:CDS 60.0%
TCGGAAGGCCGCACAAGAGA+AGG - chr8_3:4569858-4569877 None:intergenic 60.0%
TTAGGGGTAGGCGGCAGCAA+TGG + chr8_3:4569904-4569923 Msa1271130:intron 60.0%
AATGGTCTGCAGTCCGCCAC+TGG + chr8_3:4569922-4569941 Msa1271130:intron 60.0%
! AGTCCGCCACTGGCTACTTG+AGG + chr8_3:4569932-4569951 Msa1271130:intron 60.0%
! GTCCGCCACTGGCTACTTGA+GGG + chr8_3:4569933-4569952 Msa1271130:intron 60.0%
!! TGGTGCAGGCTTTGGTGGTG+AGG + chr8_3:4570310-4570329 Msa1271130:intron 60.0%
! GTGGAAGGGGTTGTGGTGGG+TGG + chr8_3:4568817-4568836 Msa1271130:intron 65.0%
!! GAAGGGGTTGTGGTGGGTGG+TGG + chr8_3:4568820-4568839 Msa1271130:intron 65.0%
!! GGTTGTGGTGGGTGGTGGTG+TGG + chr8_3:4568825-4568844 Msa1271130:intron 65.0%
AGCCATAGTGGCTGCGGCTG+CGG + chr8_3:4569592-4569611 Msa1271130:intron 65.0%
AGCCGCAGCCGCAGCCACTA+TGG - chr8_3:4569597-4569616 None:intergenic 70.0%
! GAAGGAATGGGGGGAGGCGC+AGG + chr8_3:4570245-4570264 Msa1271130:CDS 70.0%
AGTGGCTGCGGCTGCGGCTG+CGG + chr8_3:4569598-4569617 Msa1271130:intron 75.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 4568028 4570592 4568028 ID=Msa1271130;Name=Msa1271130
chr8_3 mRNA 4568028 4570592 4568028 ID=Msa1271130-mRNA-1;Parent=Msa1271130;Name=Msa1271130-mRNA-1;_AED=0.08;_eAED=0.08;_QI=231|1|0.75|1|0.66|0.5|4|0|161
chr8_3 exon 4568028 4568237 4568028 ID=Msa1271130-mRNA-1:exon:1562;Parent=Msa1271130-mRNA-1
chr8_3 exon 4569210 4569383 4569210 ID=Msa1271130-mRNA-1:exon:1563;Parent=Msa1271130-mRNA-1
chr8_3 exon 4570158 4570326 4570158 ID=Msa1271130-mRNA-1:exon:1564;Parent=Msa1271130-mRNA-1
chr8_3 exon 4570429 4570592 4570429 ID=Msa1271130-mRNA-1:exon:1565;Parent=Msa1271130-mRNA-1
chr8_3 five_prime_UTR 4568028 4568237 4568028 ID=Msa1271130-mRNA-1:five_prime_utr;Parent=Msa1271130-mRNA-1
chr8_3 five_prime_UTR 4569210 4569230 4569210 ID=Msa1271130-mRNA-1:five_prime_utr;Parent=Msa1271130-mRNA-1
chr8_3 CDS 4569231 4569383 4569231 ID=Msa1271130-mRNA-1:cds;Parent=Msa1271130-mRNA-1
chr8_3 CDS 4570158 4570326 4570158 ID=Msa1271130-mRNA-1:cds;Parent=Msa1271130-mRNA-1
chr8_3 CDS 4570429 4570592 4570429 ID=Msa1271130-mRNA-1:cds;Parent=Msa1271130-mRNA-1
Gene Sequence

>Msa1271130

ATGTTTGGGCGCGGACCAACAAGGAAGAGTGATAACACCAAATATTACGATATTCTTGGTGTTTCAAAAAGTGCTAGTGAAGATGAAATCAAGAAAGCCTATAGAAAGGCAGCGATGAAGAACCATCCAGATAAGGGTGGGGATCCTGAGAAGTTCAAGGAGTTGGGCCAAGCATATGAAGTGTTGAGCGATCCTGAAAAGAAAGAACTGTATGATCAATATGGTGAAGATGCCCTTAAAGAAGGAATGGGGGGAGGCGCAGGAAGCTCATTTCATAATCCGTTTGATATTTTCGAATCATTTTTTGGTGCAGGCTTTGGTGGTGGTGGTCCTTCACGCGCAAGAAGACAGAAGCAAGGAGAAGATGTGGTGCATTCTATAAAGGTTTCCTTGGAGGATGTGTATAACGGCACTACAAAGAAGCTATCACTTTCTAGGAATGCACTGTGCTCAAAATGTAAAGGGTACGGTCTTATCCATGCTTAA
Protein sequence

>Msa1271130

MFGRGPTRKSDNTKYYDILGVSKSASEDEIKKAYRKAAMKNHPDKGGDPEKFKELGQAYEVLSDPEKKELYDQYGEDALKEGMGGGAGSSFHNPFDIFESFFGAGFGGGGPSRARRQKQGEDVVHSIKVSLEDVYNGTTKKLSLSRNALCSKCKGYGLIHA*