AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1177560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1177560 MtrunA17_Chr8g0348161 91.781 219 18 0 1 219 1 219 1.25e-137 384
Msa1177560 MtrunA17_Chr8g0334401 38.378 185 111 3 36 219 10 192 1.19e-34 122
Msa1177560 MtrunA17_Chr8g0387601 37.104 221 120 6 1 208 1 215 5.23e-32 116
Msa1177560 MtrunA17_Chr7g0245381 35.152 165 104 3 57 219 48 211 4.50e-26 100
Msa1177560 MtrunA17_Chr2g0322771 27.749 191 126 4 32 219 3 184 1.29e-18 80.1
Msa1177560 MtrunA17_Chr4g0024221 30.928 194 116 6 32 219 3 184 2.46e-15 71.2
Msa1177560 MtrunA17_Chr5g0394271 30.208 192 119 5 33 219 9 190 1.94e-14 68.9
Msa1177560 MtrunA17_Chr2g0322791 26.562 192 128 5 32 219 3 185 4.13e-13 65.5
Msa1177560 MtrunA17_Chr8g0388291 24.706 170 107 3 58 213 46 208 1.36e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1177560 AT2G46150.1 40.708 226 122 6 1 219 1 221 2.72e-51 166
Msa1177560 AT3G54200.1 37.113 194 120 2 27 219 43 235 2.93e-21 88.6
Msa1177560 AT1G64065.1 26.442 208 146 4 10 213 9 213 4.76e-18 79.7
Msa1177560 AT3G05975.1 28.866 194 130 6 29 219 1 189 1.03e-17 78.2
Msa1177560 AT4G23610.1 31.401 207 126 9 5 199 11 213 1.05e-16 76.3

Find 41 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTCATTTGTTGTTACTTT+TGG 0.140351 8_1:+9939675 None:intergenic
CTTCTTGGCTGGACTCTAAC+TGG 0.216242 8_1:+9939837 None:intergenic
TGTACTGTTTCCAAGCTTAA+TGG 0.225190 8_1:+9939545 None:intergenic
TATTTCAGAGAGAGGAATTC+AGG 0.275802 8_1:-9939265 Msa1177560:CDS
CGCTTCTAGTCCGGATTTGT+TGG 0.282259 8_1:-9939406 Msa1177560:CDS
GAATACTTACTCCGTAATTC+CGG 0.295528 8_1:-9939355 Msa1177560:CDS
GAATGTTACGATTGATGTTA+TGG 0.346203 8_1:-9939439 Msa1177560:CDS
CTTCATCATCACTTCTTGGC+TGG 0.358214 8_1:+9939826 None:intergenic
GCTTCTAGTCCGGATTTGTT+GGG 0.369515 8_1:-9939405 Msa1177560:CDS
GTCATGTTGATCTTAACTTT+AGG 0.369852 8_1:+9939609 None:intergenic
CTTCACTCTCCCCGGAATTA+CGG 0.377348 8_1:+9939344 None:intergenic
AATACTTACTCCGTAATTCC+GGG 0.411927 8_1:-9939354 Msa1177560:CDS
TTCTTGGCTGGACTCTAACT+GGG 0.425971 8_1:+9939838 None:intergenic
TTGATCTTAACTTTAGGTGT+TGG 0.437521 8_1:+9939615 None:intergenic
GGCTAGAGGTCTTGCTTGAT+CGG 0.453925 8_1:+9939860 None:intergenic
TTCTTCGCCTTCACAAGTCC+CGG 0.466933 8_1:+9939474 None:intergenic
AACAAAATCTTCACTCTCCC+CGG 0.470476 8_1:+9939336 None:intergenic
AATTGTTATCTTAGCATTCA+CGG 0.478347 8_1:-9939715 Msa1177560:CDS
CGCGGACGCGGTAACACCAC+CGG 0.479631 8_1:-9939493 Msa1177560:CDS
CTTCTAGTCCGGATTTGTTG+GGG 0.484689 8_1:-9939404 Msa1177560:CDS
AATATAGCATCCATTAAGCT+TGG 0.490745 8_1:-9939555 Msa1177560:CDS
GGACATGATATGCACACATA+AGG 0.522877 8_1:-9939244 Msa1177560:CDS
TGACGTCAAAGTTTGTGAGT+TGG 0.525334 8_1:+9939652 None:intergenic
ACGATTAATATTTCAGAGAG+AGG 0.544282 8_1:-9939273 Msa1177560:CDS
TGGACTCTAACTGGGGCTAG+AGG 0.553580 8_1:+9939846 None:intergenic
AACACCACCGGGACTTGTGA+AGG 0.575996 8_1:-9939481 Msa1177560:CDS
TGGTATAACTGTCGCGGACG+CGG 0.579920 8_1:-9939505 Msa1177560:CDS
TCTTGGCTGGACTCTAACTG+GGG 0.600978 8_1:+9939839 None:intergenic
TTCTAGTCCGGATTTGTTGG+GGG 0.601841 8_1:-9939403 Msa1177560:CDS
GCGGACGCGGTAACACCACC+GGG 0.615153 8_1:-9939492 Msa1177560:CDS
AATAGACATGTTCACTACCA+TGG 0.631088 8_1:+9939587 None:intergenic
TTCGCCTTCACAAGTCCCGG+TGG 0.640171 8_1:+9939477 None:intergenic
CTACCGTGGTATAACTGTCG+CGG 0.657894 8_1:-9939511 Msa1177560:CDS
ACAAATCCGGACTAGAAGCG+AGG 0.679933 8_1:+9939409 None:intergenic
AGTTAAGATCAACATGACCA+TGG 0.680037 8_1:-9939604 Msa1177560:CDS
TGAAGGCGAAGAAGACAACA+AGG 0.693934 8_1:-9939464 Msa1177560:CDS
ATACTTACTCCGTAATTCCG+GGG 0.704579 8_1:-9939353 Msa1177560:CDS
ACAACAACGGTGTACTACCG+TGG 0.719084 8_1:-9939525 Msa1177560:CDS
GCTTGGAAACAGTACAACAA+CGG 0.719713 8_1:-9939538 Msa1177560:CDS
TGTTACGATTGATGTTATGG+CGG 0.723229 8_1:-9939436 Msa1177560:CDS
AATCCGGACTAGAAGCGAGG+CGG 0.753091 8_1:+9939412 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAACTTGATTTTTCTTCTA+CGG + chr8_1:9939325-9939344 None:intergenic 20.0%
! AGAAGAAAAATCAAGTTATG+TGG - chr8_1:9939326-9939345 Msa1177560:CDS 25.0%
!! TGCAATTTTACTACTACTTT+TGG - chr8_1:9939358-9939377 Msa1177560:CDS 25.0%
! AATTGTTATCTTAGCATTCA+CGG - chr8_1:9939388-9939407 Msa1177560:CDS 25.0%
!!! ATCTCATTTGTTGTTACTTT+TGG + chr8_1:9939431-9939450 None:intergenic 25.0%
! TTTTCTTCATCATCACTTCT+TGG + chr8_1:9939284-9939303 None:intergenic 30.0%
TTGATCTTAACTTTAGGTGT+TGG + chr8_1:9939491-9939510 None:intergenic 30.0%
GTCATGTTGATCTTAACTTT+AGG + chr8_1:9939497-9939516 None:intergenic 30.0%
AATATAGCATCCATTAAGCT+TGG - chr8_1:9939548-9939567 Msa1177560:CDS 30.0%
GAATGTTACGATTGATGTTA+TGG - chr8_1:9939664-9939683 Msa1177560:CDS 30.0%
ACGATTAATATTTCAGAGAG+AGG - chr8_1:9939830-9939849 Msa1177560:CDS 30.0%
! GATTTTTCTTCTACGGTTCA+TGG + chr8_1:9939318-9939337 None:intergenic 35.0%
! CTTAGCATTCACGGTTTTTA+AGG - chr8_1:9939397-9939416 Msa1177560:CDS 35.0%
AGTTAAGATCAACATGACCA+TGG - chr8_1:9939499-9939518 Msa1177560:CDS 35.0%
AATAGACATGTTCACTACCA+TGG + chr8_1:9939519-9939538 None:intergenic 35.0%
TGTACTGTTTCCAAGCTTAA+TGG + chr8_1:9939561-9939580 None:intergenic 35.0%
TGTTACGATTGATGTTATGG+CGG - chr8_1:9939667-9939686 Msa1177560:CDS 35.0%
GAATACTTACTCCGTAATTC+CGG - chr8_1:9939748-9939767 Msa1177560:CDS 35.0%
AATACTTACTCCGTAATTCC+GGG - chr8_1:9939749-9939768 Msa1177560:CDS 35.0%
TATTTCAGAGAGAGGAATTC+AGG - chr8_1:9939838-9939857 Msa1177560:CDS 35.0%
TGACGTCAAAGTTTGTGAGT+TGG + chr8_1:9939454-9939473 None:intergenic 40.0%
GCTTGGAAACAGTACAACAA+CGG - chr8_1:9939565-9939584 Msa1177560:CDS 40.0%
ATACTTACTCCGTAATTCCG+GGG - chr8_1:9939750-9939769 Msa1177560:CDS 40.0%
AACAAAATCTTCACTCTCCC+CGG + chr8_1:9939770-9939789 None:intergenic 40.0%
GGACATGATATGCACACATA+AGG - chr8_1:9939859-9939878 Msa1177560:CDS 40.0%
TTCTTGGCTGGACTCTAACT+GGG + chr8_1:9939268-9939287 None:intergenic 45.0%
CTTCATCATCACTTCTTGGC+TGG + chr8_1:9939280-9939299 None:intergenic 45.0%
TGAAGGCGAAGAAGACAACA+AGG - chr8_1:9939639-9939658 Msa1177560:CDS 45.0%
! GCTTCTAGTCCGGATTTGTT+GGG - chr8_1:9939698-9939717 Msa1177560:CDS 45.0%
!! CTTCTAGTCCGGATTTGTTG+GGG - chr8_1:9939699-9939718 Msa1177560:CDS 45.0%
!! TTCTAGTCCGGATTTGTTGG+GGG - chr8_1:9939700-9939719 Msa1177560:CDS 45.0%
TTTGTTGGGGGATGTGCTTA+AGG - chr8_1:9939712-9939731 Msa1177560:CDS 45.0%
TTGTTGGGGGATGTGCTTAA+GGG - chr8_1:9939713-9939732 Msa1177560:CDS 45.0%
GGCTAGAGGTCTTGCTTGAT+CGG + chr8_1:9939246-9939265 None:intergenic 50.0%
TCTTGGCTGGACTCTAACTG+GGG + chr8_1:9939267-9939286 None:intergenic 50.0%
CTTCTTGGCTGGACTCTAAC+TGG + chr8_1:9939269-9939288 None:intergenic 50.0%
ACAACAACGGTGTACTACCG+TGG - chr8_1:9939578-9939597 Msa1177560:CDS 50.0%
CTACCGTGGTATAACTGTCG+CGG - chr8_1:9939592-9939611 Msa1177560:CDS 50.0%
TTCTTCGCCTTCACAAGTCC+CGG + chr8_1:9939632-9939651 None:intergenic 50.0%
! ACAAATCCGGACTAGAAGCG+AGG + chr8_1:9939697-9939716 None:intergenic 50.0%
CGCTTCTAGTCCGGATTTGT+TGG - chr8_1:9939697-9939716 Msa1177560:CDS 50.0%
AGCACATCCCCCAACAAATC+CGG + chr8_1:9939710-9939729 None:intergenic 50.0%
TGTTGGGGGATGTGCTTAAG+GGG - chr8_1:9939714-9939733 Msa1177560:CDS 50.0%
CTTCACTCTCCCCGGAATTA+CGG + chr8_1:9939762-9939781 None:intergenic 50.0%
TGGACTCTAACTGGGGCTAG+AGG + chr8_1:9939260-9939279 None:intergenic 55.0%
CGTCCGCGACAGTTATACCA+CGG + chr8_1:9939598-9939617 None:intergenic 55.0%
TGGTATAACTGTCGCGGACG+CGG - chr8_1:9939598-9939617 Msa1177560:CDS 55.0%
AACACCACCGGGACTTGTGA+AGG - chr8_1:9939622-9939641 Msa1177560:CDS 55.0%
! AATCCGGACTAGAAGCGAGG+CGG + chr8_1:9939694-9939713 None:intergenic 55.0%
GTTGGGGGATGTGCTTAAGG+GGG - chr8_1:9939715-9939734 Msa1177560:CDS 55.0%
GGATGTGCTTAAGGGGGAGA+TGG - chr8_1:9939721-9939740 Msa1177560:CDS 55.0%
TTCGCCTTCACAAGTCCCGG+TGG + chr8_1:9939629-9939648 None:intergenic 60.0%
GGACCGCCTCGCTTCTAGTC+CGG - chr8_1:9939688-9939707 Msa1177560:CDS 65.0%
CGCGGACGCGGTAACACCAC+CGG - chr8_1:9939610-9939629 Msa1177560:CDS 70.0%
GCGGACGCGGTAACACCACC+GGG - chr8_1:9939611-9939630 Msa1177560:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 9939233 9939892 9939233 ID=Msa1177560;Name=Msa1177560
chr8_1 mRNA 9939233 9939892 9939233 ID=Msa1177560-mRNA-1;Parent=Msa1177560;Name=Msa1177560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|219
chr8_1 exon 9939233 9939892 9939233 ID=Msa1177560-mRNA-1:exon:3418;Parent=Msa1177560-mRNA-1
chr8_1 CDS 9939233 9939892 9939233 ID=Msa1177560-mRNA-1:cds;Parent=Msa1177560-mRNA-1
Gene Sequence

>Msa1177560

ATGGCAGAGACCGATCAAGCAAGACCTCTAGCCCCAGTTAGAGTCCAGCCAAGAAGTGATGATGAAGAAAAAGCTCTTAAAACCATGAACCGTAGAAGAAAAATCAAGTTATGTGGTTGTGTAACTGCAATTTTACTACTACTTTTGGTTATAGTAATTGTTATCTTAGCATTCACGGTTTTTAAGGTTAAAGATCCAAAAGTAACAACAAATGAGATCCAACTCACAAACTTTGACGTCAACGTTGTTCAAATTCCAACACCTAAAGTTAAGATCAACATGACCATGGTAGTGAACATGTCTATTAAGAATGATAATATAGCATCCATTAAGCTTGGAAACAGTACAACAACGGTGTACTACCGTGGTATAACTGTCGCGGACGCGGTAACACCACCGGGACTTGTGAAGGCGAAGAAGACAACAAGGTTGAATGTTACGATTGATGTTATGGCGGACCGCCTCGCTTCTAGTCCGGATTTGTTGGGGGATGTGCTTAAGGGGGAGATGGTGATGAATACTTACTCCGTAATTCCGGGGAGAGTGAAGATTTTGTTTATTAAGAAACATGTTGAGATAATGATGAATTGTACTATGACGATTAATATTTCAGAGAGAGGAATTCAGGACATGATATGCACACATAAGGTGAAACTTTAG

Protein sequence

>Msa1177560

MAETDQARPLAPVRVQPRSDDEEKALKTMNRRRKIKLCGCVTAILLLLLVIVIVILAFTVFKVKDPKVTTNEIQLTNFDVNVVQIPTPKVKINMTMVVNMSIKNDNIASIKLGNSTTTVYYRGITVADAVTPPGLVKAKKTTRLNVTIDVMADRLASSPDLLGDVLKGEMVMNTYSVIPGRVKILFIKKHVEIMMNCTMTINISERGIQDMICTHKVKL*