AlfalfaGEDB | Msa1068280

M. sativa cultivar zhongmu-4 / Msa1068280

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1068280	XP_003624235.1	94.048	336	17	3	1	334	1	335	0.0	599

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1068280	sp\|Q9T072\|BH025_ARATH	39.013	223	115	6	118	326	109	324	3.15e-35	133

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1068280	G7KSG4	94.048	336	17	3	1	334	1	335	0.0	599

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TFs/TRs:

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Gene ID	Type	Classification
Msa1068280	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0146740	Msa1068280	0.806617	7.541623e-50	-8.615850e-47
Msa0187030	Msa1068280	0.826071	3.316813e-54	-8.615850e-47
Msa0402180	Msa1068280	0.807766	4.305686e-50	-8.615850e-47
Msa0483800	Msa1068280	0.826790	2.235439e-54	-8.615850e-47
Msa0579660	Msa1068280	0.807160	5.787704e-50	-8.615850e-47
Msa0751260	Msa1068280	0.801431	9.033147e-49	-8.615850e-47
Msa0795070	Msa1068280	0.822924	1.825412e-53	-8.615850e-47
Msa1029420	Msa1068280	0.983029	1.221626e-156	-8.615850e-47
Msa1033660	Msa1068280	0.827169	1.815008e-54	-8.615850e-47
Msa1038080	Msa1068280	0.808468	3.051488e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1068280	MtrunA17_Chr7g0249811	94.048	336	17	3	1	334	1	335	0.0	599
Msa1068280	MtrunA17_Chr8g0336681	45.528	246	104	5	115	332	97	340	1.14e-56	187
Msa1068280	MtrunA17_Chr4g0002801	36.986	365	159	11	1	328	1	331	3.53e-52	175
Msa1068280	MtrunA17_Chr2g0333641	36.182	351	154	9	1	326	1	306	5.17e-52	174
Msa1068280	MtrunA17_Chr2g0333621	36.901	355	152	10	1	327	1	311	3.24e-51	172
Msa1068280	MtrunA17_Chr4g0002781	35.121	373	174	11	1	328	1	350	2.64e-49	168
Msa1068280	MtrunA17_Chr5g0400661	35.461	282	133	5	95	327	53	334	7.44e-43	150
Msa1068280	MtrunA17_Chr5g0400621	42.408	191	101	1	148	329	141	331	5.05e-41	146
Msa1068280	MtrunA17_Chr4g0054561	45.251	179	91	2	155	326	146	324	2.07e-40	144
Msa1068280	MtrunA17_Chr4g0049331	40.164	244	123	6	101	323	62	303	4.30e-40	143

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1068280	AT4G37850.2	39.013	223	115	6	118	326	109	324	3.20e-36	133
Msa1068280	AT4G37850.1	39.013	223	115	6	118	326	109	324	3.20e-36	133
Msa1068280	AT2G22770.1	40.609	197	93	2	156	328	124	320	8.85e-36	132
Msa1068280	AT2G22750.2	38.636	264	117	7	73	322	65	297	1.35e-32	123
Msa1068280	AT2G22750.5	38.636	264	117	7	73	322	89	321	2.40e-32	123
Msa1068280	AT2G22750.3	38.636	264	117	7	73	322	93	325	2.59e-32	123
Msa1068280	AT2G22760.1	40.556	180	94	3	157	323	112	291	3.11e-32	122
Msa1068280	AT2G22750.1	39.015	264	115	8	73	322	65	296	8.61e-32	121
Msa1068280	AT2G22750.4	39.015	264	115	8	73	322	93	324	1.27e-31	121
Msa1068280	AT2G22760.2	38.650	163	88	2	157	307	112	274	6.53e-27	107

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Find 78 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCTTGCTCATCAAATG+TGG	0.646764	7_2:-63264002	None:intergenic
AAAGATAGAAGGTATGTGGC+TGG	0.543699	7_2:-63264274	None:intergenic
AAAGGACAAAATACTATTTG+TGG	0.476362	7_2:-63264146	None:intergenic
AAATAAACTTGTCTCATCAA+AGG	0.579876	7_2:-63264164	None:intergenic
AAATCTGAGAAAATATCTAT+TGG	0.423346	7_2:+63266436	Msa1068280:CDS
AACCGAGCCTCCATATTCGC+AGG	0.555589	7_2:-63266817	None:intergenic
AAGATCACTTAATGGCTGAG+AGG	0.675984	7_2:+63264416	Msa1068280:CDS
AAGCAACAAAGAAGGTTAGA+AGG	0.446951	7_2:+63264377	Msa1068280:CDS
AAGGACAAAATACTATTTGT+GGG	0.492056	7_2:-63264145	None:intergenic
AATCTCAAGCAGTGATTCTG+AGG	0.530393	7_2:-63265400	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTATACCAGTCATATTCAAA+AGG	+	chr7_2:63263774-63263793	Msa1068280:five_prime_UTR	30.0%
TTGGAACAAAGATAAGAAGA+GGG	-	chr7_2:63264067-63264086	None:intergenic	30.0%
ATTGGAACAAAGATAAGAAG+AGG	-	chr7_2:63264068-63264087	None:intergenic	30.0%
TTGAGTTACCAAAAGATAGA+AGG	-	chr7_2:63264288-63264307	None:intergenic	30.0%
AATTCATTGTGAGAAGCAAA+AGG	+	chr7_2:63265458-63265477	Msa1068280:CDS	30.0%
TCTGAGAAAATATCTATTGG+AGG	+	chr7_2:63266439-63266458	Msa1068280:CDS	30.0%
CCAATCAAAGAAAGTAGAAA+TGG	-	chr7_2:63266559-63266578	None:intergenic	30.0%
CCATTTCTACTTTCTTTGAT+TGG	+	chr7_2:63266556-63266575	Msa1068280:three_prime_UTR	30.0%
AGTATATTCAAATCGCATCT+GGG	+	chr7_2:63266739-63266758	Msa1068280:three_prime_UTR	30.0%
ACAACATGGTTATTAATCAC+AGG	+	chr7_2:63266788-63266807	Msa1068280:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7_2	gene	63263764	63266855	63263764	ID=Msa1068280;Name=Msa1068280
chr7_2	mRNA	63263764	63266855	63263764	ID=Msa1068280-mRNA-1;Parent=Msa1068280;Name=Msa1068280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=65\|1\|1\|1\|1\|1\|4\|374\|334
chr7_2	exon	63263764	63263864	63263764	ID=Msa1068280-mRNA-1:exon:13004;Parent=Msa1068280-mRNA-1
chr7_2	exon	63263965	63264504	63263965	ID=Msa1068280-mRNA-1:exon:13005;Parent=Msa1068280-mRNA-1
chr7_2	exon	63265249	63265590	63265249	ID=Msa1068280-mRNA-1:exon:13006;Parent=Msa1068280-mRNA-1
chr7_2	exon	63266395	63266855	63266395	ID=Msa1068280-mRNA-1:exon:13007;Parent=Msa1068280-mRNA-1
chr7_2	five_prime_UTR	63263764	63263828	63263764	ID=Msa1068280-mRNA-1:five_prime_utr;Parent=Msa1068280-mRNA-1
chr7_2	CDS	63263829	63263864	63263829	ID=Msa1068280-mRNA-1:cds;Parent=Msa1068280-mRNA-1
chr7_2	CDS	63263965	63264504	63263965	ID=Msa1068280-mRNA-1:cds;Parent=Msa1068280-mRNA-1
chr7_2	CDS	63265249	63265590	63265249	ID=Msa1068280-mRNA-1:cds;Parent=Msa1068280-mRNA-1
chr7_2	CDS	63266395	63266481	63266395	ID=Msa1068280-mRNA-1:cds;Parent=Msa1068280-mRNA-1
chr7_2	three_prime_UTR	63266482	63266855	63266482	ID=Msa1068280-mRNA-1:three_prime_utr;Parent=Msa1068280-mRNA-1

Gene Sequence

>Msa1068280

ATGGAGGATTCACTGGAGAATTTGATTTCTTATATGGAAATGGAAGATGATGTCTTGAATCAAAGTACCACCACCACATTTGATGAGCAAGAGTTTCTCAAAGATATCATCCTTGAAGAACCAGAATCATGTGAACCCTCTTCTTATCTTTGTTCCAATAAAACCCAAGGCAATAGTACAACAACTATAATAAATGTTGAAGGTGATGCGACTAGTCCCACAAATAGTATTTTGTCCTTTGATGAGACAAGTTTATTTTGTGGTGACTATGAGAATGTTGAAACAAACCACAAAAGTAATAGCTCCAACTCAATCAAGTCTTTGGAAAGATCTTGTGTTTCTTCTCCAGCCACATACCTTCTATCTTTTGGTAACTCAAGTATTGAACCAATCATTGAACCAATGTCACATAAAACTAAAAGAAGGACAGATGAAGCAAGAGTGAAGGAAGCAACAAAGAAGGTTAGAAGGTCATGTGAGACAGTACAAGATCACTTAATGGCTGAGAGGAAGAGGAGAAGGGAATTAACAGAGAATATCATAGCACTTTCAGCCATGATACCTGGCTTGAAAAAGATGGACAAGTGTTATGTACTTAGTGAAGCTGTGAATCACACAAAACAGCTTCAAAAGCGCATTAAAGAATTGGAGAATCAAAATAAAGACAACAAAGCAAATCCAGCAATATTCAAGTGGAAATCTCAAGTTTCATCAAATAAAAAGAAATCCTCAGAATCACTGCTTGAGATTGAAGCTAGAGTCAAAGAAAAGGAAGTACTCATCAGAATTCATTGTGAGAAGCAAAAGGACATAGTGCTTAAAATACATGAATTGCTTGAAAAGTTCAATCTCACTATAACAAGTAGTAGCATGTTACCATTCGGTGCTTCTATTCTTGTAATCAACATTTGTGCTCAGATGGATGAAGAAGACAGCATGGCCATGGATGACCTTGTGGAAAATCTGAGAAAATATCTATTGGAGGCACATGAGAGTCAATTGTGA

Protein sequence

>Msa1068280

MEDSLENLISYMEMEDDVLNQSTTTTFDEQEFLKDIILEEPESCEPSSYLCSNKTQGNSTTTIINVEGDATSPTNSILSFDETSLFCGDYENVETNHKSNSSNSIKSLERSCVSSPATYLLSFGNSSIEPIIEPMSHKTKRRTDEARVKEATKKVRRSCETVQDHLMAERKRRRELTENIIALSAMIPGLKKMDKCYVLSEAVNHTKQLQKRIKELENQNKDNKANPAIFKWKSQVSSNKKKSSESLLEIEARVKEKEVLIRIHCEKQKDIVLKIHELLEKFNLTITSSSMLPFGASILVINICAQMDEEDSMAMDDLVENLRKYLLEAHESQL*