AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1070620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1070620 MtrunA17_Chr7g0253101 94.834 271 4 3 1 271 1 261 0.0 509
Msa1070620 MtrunA17_Chr6g0461591 62.409 274 73 10 1 261 1 257 4.23e-104 303
Msa1070620 MtrunA17_Chr3g0140331 39.200 250 95 9 15 260 121 317 5.02e-35 129
Msa1070620 MtrunA17_Chr1g0199671 76.471 68 16 0 193 260 239 306 5.26e-34 125
Msa1070620 MtrunA17_Chr4g0035891 71.795 78 19 1 196 270 211 288 1.80e-33 123
Msa1070620 MtrunA17_Chr2g0326771 57.944 107 39 2 172 272 191 297 1.82e-33 124
Msa1070620 MtrunA17_Chr5g0404981 41.176 238 100 9 45 269 163 373 2.74e-33 125
Msa1070620 MtrunA17_Chr8g0372291 77.465 71 16 0 196 266 218 288 3.60e-33 122
Msa1070620 MtrunA17_Chr1g0146231 35.985 264 114 9 11 260 76 298 7.27e-33 123
Msa1070620 MtrunA17_Chr8g0340401 37.118 229 78 7 45 260 115 290 3.07e-32 121
Msa1070620 MtrunA17_Chr1g0213161 75.758 66 16 0 196 261 152 217 6.53e-32 117
Msa1070620 MtrunA17_Chr1g0169431 74.648 71 18 0 190 260 166 236 6.59e-32 118
Msa1070620 MtrunA17_Chr4g0028961 72.222 72 20 0 193 264 229 300 9.60e-32 119
Msa1070620 MtrunA17_Chr3g0145121 70.833 72 21 0 196 267 215 286 1.60e-31 118
Msa1070620 MtrunA17_Chr5g0395391 73.846 65 17 0 196 260 270 334 3.13e-29 113
Msa1070620 MtrunA17_Chr5g0395391 71.875 64 18 0 196 259 168 231 1.44e-27 108
Msa1070620 MtrunA17_Chr7g0232671 58.065 62 26 0 196 257 187 248 6.00e-20 86.7
Msa1070620 MtrunA17_Chr7g0232701 56.923 65 28 0 193 257 70 134 7.63e-20 83.2
Msa1070620 MtrunA17_Chr7g0232681 40.000 105 63 0 153 257 89 193 2.92e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1070620 AT4G32890.1 42.793 222 81 6 45 260 80 261 9.64e-44 150
Msa1070620 AT2G45050.1 38.618 246 98 8 21 266 57 249 4.07e-40 140
Msa1070620 AT5G66320.2 40.816 245 112 8 28 260 90 313 3.87e-36 132
Msa1070620 AT5G66320.1 40.816 245 112 8 28 260 90 313 3.87e-36 132
Msa1070620 AT3G60530.1 40.553 217 57 9 45 260 77 222 9.13e-36 128
Msa1070620 AT5G25830.1 38.235 238 89 8 45 260 82 283 2.60e-35 129
Msa1070620 AT3G24050.1 75.000 68 17 0 193 260 191 258 4.30e-33 122
Msa1070620 AT4G36240.1 38.532 218 71 6 45 261 74 229 7.35e-33 120
Msa1070620 AT3G54810.1 77.612 67 15 0 194 260 227 293 1.78e-32 122
Msa1070620 AT3G54810.2 77.612 67 15 0 194 260 227 293 1.78e-32 122
Msa1070620 AT3G51080.1 81.538 65 12 0 196 260 221 285 5.02e-32 120
Msa1070620 AT1G08010.2 72.308 65 18 0 196 260 220 284 3.65e-31 117
Msa1070620 AT1G08010.1 72.308 65 18 0 196 260 220 284 3.65e-31 117
Msa1070620 AT1G08010.3 72.308 65 18 0 196 260 220 284 3.65e-31 117
Msa1070620 AT1G08000.2 73.846 65 17 0 196 260 218 282 7.54e-30 114
Msa1070620 AT1G08000.1 73.846 65 17 0 196 260 218 282 7.54e-30 114
Msa1070620 AT4G34680.1 72.308 65 18 0 196 260 180 244 9.11e-30 113
Msa1070620 AT4G34680.2 72.308 65 18 0 196 260 180 244 9.11e-30 113
Msa1070620 AT2G28340.1 65.672 67 23 0 198 264 193 259 1.44e-26 105
Msa1070620 AT3G45170.1 56.579 76 33 0 185 260 104 179 5.25e-26 102

Find 69 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGGTGAAATTGACTATT+TGG 0.123062 7_2:-66938447 Msa1070620:three_prime_UTR
CTCTCTTAAAACAAGCTTAT+TGG 0.124697 7_2:-66938908 Msa1070620:CDS
CACTAGAAACTTGTACATTT+TGG 0.144962 7_2:+66939631 None:intergenic
AACTTAGAGAGCACCAATTT+TGG 0.237029 7_2:-66939754 Msa1070620:five_prime_UTR
CATGCATTGCATAGTGTCTT+TGG 0.243410 7_2:+66938675 None:intergenic
TGAAAAGGAAATTGTGAAAA+AGG 0.260278 7_2:-66938820 Msa1070620:CDS
CATAGTGTCTTTGGGCCTAA+TGG 0.278012 7_2:+66938684 None:intergenic
ACACTACTCACTAGGATTAA+TGG 0.294145 7_2:+66938518 None:intergenic
GGAAACATGAGATAGTATTT+AGG 0.308712 7_2:-66938426 Msa1070620:three_prime_UTR
ATGGGCCTGCCCTCCATTGT+GGG 0.318681 7_2:+66938703 None:intergenic
CAATGGAGGGCAGGCCCATT+AGG 0.322478 7_2:-66938699 Msa1070620:CDS
AAGATGCTTAAAGGGTCTTT+TGG 0.328925 7_2:+66938978 None:intergenic
GTTGTTAGTGCTATGATTAT+TGG 0.333550 7_2:-66938400 Msa1070620:three_prime_UTR
GTATTGGATAAGATGCTTAA+AGG 0.338824 7_2:+66938969 None:intergenic
ATAGTGTCTTTGGGCCTAAT+GGG 0.339035 7_2:+66938685 None:intergenic
CACACCAAGAGAGGGGAAAA+TGG 0.339492 7_2:+66939709 None:intergenic
GGTTTCTTCACTGTGTTTGA+AGG 0.343748 7_2:+66939083 None:intergenic
AGAGAAAAGAAAGCCAAAAT+TGG 0.351490 7_2:+66939741 None:intergenic
GTACACCTCCTTGTTGGAAT+TGG 0.356244 7_2:+66938747 None:intergenic
TATGAAAGTGAAATGCAATA+TGG 0.367111 7_2:+66938307 None:intergenic
ATGCATTGCATAGTGTCTTT+GGG 0.368727 7_2:+66938676 None:intergenic
AATGGGCCTGCCCTCCATTG+TGG 0.406749 7_2:+66938702 None:intergenic
AGAAAGAGACAAAATGAAAC+AGG 0.417263 7_2:+66938347 None:intergenic
TATTGGATAAGATGCTTAAA+GGG 0.429212 7_2:+66938970 None:intergenic
CAATGTGTACACCTCCTTGT+TGG 0.437336 7_2:+66938741 None:intergenic
AGATTATGAAGTGGTTGAAA+AGG 0.475929 7_2:-66938835 Msa1070620:CDS
GTAAAAGTACACAACTTTGT+GGG 0.484296 7_2:+66939147 None:intergenic
TAGTGAACTCATGGTACCTA+AGG 0.486078 7_2:-66938877 Msa1070620:CDS
GGTAAAAGTACACAACTTTG+TGG 0.499157 7_2:+66939146 None:intergenic
CAGGCAAAGCAAGAAGCAAA+AGG 0.500793 7_2:-66939025 Msa1070620:CDS
TAAAAGGTGAACTGAGACAA+GGG 0.508516 7_2:-66939786 None:intergenic
AGGACCCCACAATGGAGGGC+AGG 0.512119 7_2:-66938708 Msa1070620:CDS
ATTATGTAGGAAGTGGATGT+TGG 0.517271 7_2:-66939212 Msa1070620:intron
ATCCTAGTGAGTAGTGTTGG+TGG 0.530927 7_2:-66938512 Msa1070620:exon
TTGAAAGGTTAGAGCAAGAA+GGG 0.531774 7_2:+66939583 None:intergenic
CTTGAAAGGTTAGAGCAAGA+AGG 0.532182 7_2:+66939582 None:intergenic
ACAAAAGTTGGACTCTTAGC+AGG 0.532481 7_2:+66938609 None:intergenic
TAGTGAGTGCAAACAAGAGA+AGG 0.541293 7_2:-66939613 Msa1070620:CDS
GTTAGCTGATAGTGAACTCA+TGG 0.566356 7_2:-66938886 Msa1070620:CDS
TGGGCCTGCCCTCCATTGTG+GGG 0.567754 7_2:+66938704 None:intergenic
GGTCATCAAGGCTACTTGAA+AGG 0.569542 7_2:+66939568 None:intergenic
GAGCAGAAAAGAGGTCATCA+AGG 0.570986 7_2:+66939556 None:intergenic
CAAGAGAGGGGAAAATGGTA+AGG 0.576200 7_2:+66939714 None:intergenic
TGTGGTGTGAGGTACAAATC+TGG 0.576464 7_2:-66938654 Msa1070620:CDS
CTCTTAGCAGGTCTATACTC+TGG 0.579993 7_2:+66938621 None:intergenic
GTAGGAAGTGGATGTTGGAA+TGG 0.583317 7_2:-66939207 Msa1070620:intron
TTAATCCTAGTGAGTAGTGT+TGG 0.586069 7_2:-66938515 Msa1070620:exon
TTTGAGGTTGATGTTAACAA+TGG 0.593552 7_2:-66938777 Msa1070620:CDS
ACAAAGGACCCCACAATGGA+GGG 0.595325 7_2:-66938712 Msa1070620:CDS
TAATTTGAACACACCAAGAG+AGG 0.599833 7_2:+66939700 None:intergenic
CACAAAGGACCCCACAATGG+AGG 0.605327 7_2:-66938713 Msa1070620:CDS
ACAAGAAAGTCATGGAGATG+AGG 0.609025 7_2:-66938563 Msa1070620:CDS
AAATTCACACAAGAAAGTCA+TGG 0.613481 7_2:-66938571 Msa1070620:CDS
AATTTGAACACACCAAGAGA+GGG 0.616813 7_2:+66939701 None:intergenic
GGTAACAAAAGATTATGAAG+TGG 0.619135 7_2:-66938844 Msa1070620:CDS
ATATGGTATGCAACATGAGG+AGG 0.623003 7_2:+66938324 None:intergenic
AGTGAACTCATGGTACCTAA+GGG 0.625585 7_2:-66938876 Msa1070620:CDS
AAGTGGATGTTGGAATGGAA+TGG 0.634243 7_2:-66939202 Msa1070620:CDS
GCAATATGGTATGCAACATG+AGG 0.637604 7_2:+66938321 None:intergenic
AACCACCAACACTACTCACT+AGG 0.638539 7_2:+66938510 None:intergenic
GTCAAAATCCAATTCCAACA+AGG 0.638845 7_2:-66938755 Msa1070620:CDS
ATAAAAGGTGAACTGAGACA+AGG 0.649757 7_2:-66939787 None:intergenic
GTACACATTGTTTGTCACAA+AGG 0.655851 7_2:-66938728 Msa1070620:CDS
GTGTGAGGTACAAATCTGGT+AGG 0.664094 7_2:-66938650 Msa1070620:CDS
ATTTGAACACACCAAGAGAG+GGG 0.671203 7_2:+66939702 None:intergenic
TGTCACAAAGGACCCCACAA+TGG 0.681006 7_2:-66938716 Msa1070620:CDS
AAAATCCAATTCCAACAAGG+AGG 0.704851 7_2:-66938752 Msa1070620:CDS
TATGCAATGCATGTGGTGTG+AGG 0.734829 7_2:-66938665 Msa1070620:CDS
AAGACACTATGCAATGCATG+TGG 0.751831 7_2:-66938672 Msa1070620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAGAAACATAATATTAA+AGG + chr7_2:66938761-66938780 None:intergenic 10.0%
!!! TAAATCAAAGTTTAAATTTT+AGG - chr7_2:66939612-66939631 Msa1070620:CDS 10.0%
!! AATTAGAAACATAATATTAA+AGG + chr7_2:66938761-66938780 None:intergenic 10.0%
!!! TAAATCAAAGTTTAAATTTT+AGG - chr7_2:66939612-66939631 Msa1070620:CDS 10.0%
!!! TATTATGTTTCTAATTTTCT+TGG - chr7_2:66938765-66938784 Msa1070620:CDS 15.0%
!!! TATTATGTTTCTAATTTTCT+TGG - chr7_2:66938765-66938784 Msa1070620:CDS 15.0%
!!! TGTAGTTTTTGCTAAAATTA+TGG - chr7_2:66938809-66938828 Msa1070620:CDS 20.0%
!!! TGTAGTTTTTGCTAAAATTA+TGG - chr7_2:66938809-66938828 Msa1070620:CDS 20.0%
! TAAGGTGTAGAAAAAGTTAA+GGG + chr7_2:66938409-66938428 None:intergenic 25.0%
! ATAAGGTGTAGAAAAAGTTA+AGG + chr7_2:66938410-66938429 None:intergenic 25.0%
!! TTTTCTACACCTTATCAAAA+TGG - chr7_2:66938415-66938434 Msa1070620:three_prime_UTR 25.0%
! GAAAAACATATGTTAGAACA+AGG + chr7_2:66938675-66938694 None:intergenic 25.0%
! TTTGACCAAACAAATTATGT+AGG - chr7_2:66938851-66938870 Msa1070620:CDS 25.0%
! TATTGGATAAGATGCTTAAA+GGG + chr7_2:66939109-66939128 None:intergenic 25.0%
! TGAAAAGGAAATTGTGAAAA+AGG - chr7_2:66939256-66939275 Msa1070620:intron 25.0%
! TTTAGGTGAAATTGACTATT+TGG - chr7_2:66939629-66939648 Msa1070620:CDS 25.0%
! TATGAAAGTGAAATGCAATA+TGG + chr7_2:66939772-66939791 None:intergenic 25.0%
! TAAGGTGTAGAAAAAGTTAA+GGG + chr7_2:66938409-66938428 None:intergenic 25.0%
! ATAAGGTGTAGAAAAAGTTA+AGG + chr7_2:66938410-66938429 None:intergenic 25.0%
!! TTTTCTACACCTTATCAAAA+TGG - chr7_2:66938415-66938434 Msa1070620:three_prime_UTR 25.0%
! GAAAAACATATGTTAGAACA+AGG + chr7_2:66938675-66938694 None:intergenic 25.0%
! TTTGACCAAACAAATTATGT+AGG - chr7_2:66938851-66938870 Msa1070620:CDS 25.0%
! TATTGGATAAGATGCTTAAA+GGG + chr7_2:66939109-66939128 None:intergenic 25.0%
! TGAAAAGGAAATTGTGAAAA+AGG - chr7_2:66939256-66939275 Msa1070620:intron 25.0%
! TTTAGGTGAAATTGACTATT+TGG - chr7_2:66939629-66939648 Msa1070620:CDS 25.0%
! TATGAAAGTGAAATGCAATA+TGG + chr7_2:66939772-66939791 None:intergenic 25.0%
AGAGAAAAGAAAGCCAAAAT+TGG + chr7_2:66938338-66938357 None:intergenic 30.0%
AAGGTGTAGAAAAAGTTAAG+GGG + chr7_2:66938408-66938427 None:intergenic 30.0%
! CACTAGAAACTTGTACATTT+TGG + chr7_2:66938448-66938467 None:intergenic 30.0%
!! CACTTCCTACATAATTTGTT+TGG + chr7_2:66938859-66938878 None:intergenic 30.0%
CAAACAAATTATGTAGGAAG+TGG - chr7_2:66938857-66938876 Msa1070620:CDS 30.0%
! GTAAAAGTACACAACTTTGT+GGG + chr7_2:66938932-66938951 None:intergenic 30.0%
!! GTTGTGTTTTGAACATTAGA+GGG + chr7_2:66938954-66938973 None:intergenic 30.0%
!! TGTTGTGTTTTGAACATTAG+AGG + chr7_2:66938955-66938974 None:intergenic 30.0%
!! GACTCATTTTGTTCTTGTTT+TGG + chr7_2:66939017-66939036 None:intergenic 30.0%
GTATTGGATAAGATGCTTAA+AGG + chr7_2:66939110-66939129 None:intergenic 30.0%
!! ATTTTGAGGGTTTAAGGTAT+TGG + chr7_2:66939126-66939145 None:intergenic 30.0%
! AACATAGTGACTCATTTTGA+GGG + chr7_2:66939139-66939158 None:intergenic 30.0%
!!! CAATAAGCTTGTTTTAAGAG+AGG + chr7_2:66939170-66939189 None:intergenic 30.0%
CTCTCTTAAAACAAGCTTAT+TGG - chr7_2:66939168-66939187 Msa1070620:CDS 30.0%
GGTAACAAAAGATTATGAAG+TGG - chr7_2:66939232-66939251 Msa1070620:intron 30.0%
AGATTATGAAGTGGTTGAAA+AGG - chr7_2:66939241-66939260 Msa1070620:intron 30.0%
AGGAAATTGTGAAAAAGGAA+AGG - chr7_2:66939261-66939280 Msa1070620:intron 30.0%
!! AAAGGAAAGGTTTGATTTTG+AGG - chr7_2:66939274-66939293 Msa1070620:intron 30.0%
!! AAGGAAAGGTTTGATTTTGA+GGG - chr7_2:66939275-66939294 Msa1070620:intron 30.0%
!! TTTGAGGTTGATGTTAACAA+TGG - chr7_2:66939299-66939318 Msa1070620:intron 30.0%
TGCAAAAAACTCACAAAAGT+TGG + chr7_2:66939482-66939501 None:intergenic 30.0%
AAATTCACACAAGAAAGTCA+TGG - chr7_2:66939505-66939524 Msa1070620:intron 30.0%
GGAAACATGAGATAGTATTT+AGG - chr7_2:66939650-66939669 Msa1070620:exon 30.0%
GTTGTTAGTGCTATGATTAT+TGG - chr7_2:66939676-66939695 Msa1070620:five_prime_UTR 30.0%
AGAAAGAGACAAAATGAAAC+AGG + chr7_2:66939732-66939751 None:intergenic 30.0%
AGAGAAAAGAAAGCCAAAAT+TGG + chr7_2:66938338-66938357 None:intergenic 30.0%
AAGGTGTAGAAAAAGTTAAG+GGG + chr7_2:66938408-66938427 None:intergenic 30.0%
! CACTAGAAACTTGTACATTT+TGG + chr7_2:66938448-66938467 None:intergenic 30.0%
!! CACTTCCTACATAATTTGTT+TGG + chr7_2:66938859-66938878 None:intergenic 30.0%
CAAACAAATTATGTAGGAAG+TGG - chr7_2:66938857-66938876 Msa1070620:CDS 30.0%
! GTAAAAGTACACAACTTTGT+GGG + chr7_2:66938932-66938951 None:intergenic 30.0%
!! GTTGTGTTTTGAACATTAGA+GGG + chr7_2:66938954-66938973 None:intergenic 30.0%
!! TGTTGTGTTTTGAACATTAG+AGG + chr7_2:66938955-66938974 None:intergenic 30.0%
!! GACTCATTTTGTTCTTGTTT+TGG + chr7_2:66939017-66939036 None:intergenic 30.0%
GTATTGGATAAGATGCTTAA+AGG + chr7_2:66939110-66939129 None:intergenic 30.0%
!! ATTTTGAGGGTTTAAGGTAT+TGG + chr7_2:66939126-66939145 None:intergenic 30.0%
! AACATAGTGACTCATTTTGA+GGG + chr7_2:66939139-66939158 None:intergenic 30.0%
!!! CAATAAGCTTGTTTTAAGAG+AGG + chr7_2:66939170-66939189 None:intergenic 30.0%
CTCTCTTAAAACAAGCTTAT+TGG - chr7_2:66939168-66939187 Msa1070620:CDS 30.0%
GGTAACAAAAGATTATGAAG+TGG - chr7_2:66939232-66939251 Msa1070620:intron 30.0%
AGATTATGAAGTGGTTGAAA+AGG - chr7_2:66939241-66939260 Msa1070620:intron 30.0%
AGGAAATTGTGAAAAAGGAA+AGG - chr7_2:66939261-66939280 Msa1070620:intron 30.0%
!! AAAGGAAAGGTTTGATTTTG+AGG - chr7_2:66939274-66939293 Msa1070620:intron 30.0%
!! AAGGAAAGGTTTGATTTTGA+GGG - chr7_2:66939275-66939294 Msa1070620:intron 30.0%
!! TTTGAGGTTGATGTTAACAA+TGG - chr7_2:66939299-66939318 Msa1070620:intron 30.0%
TGCAAAAAACTCACAAAAGT+TGG + chr7_2:66939482-66939501 None:intergenic 30.0%
AAATTCACACAAGAAAGTCA+TGG - chr7_2:66939505-66939524 Msa1070620:intron 30.0%
GGAAACATGAGATAGTATTT+AGG - chr7_2:66939650-66939669 Msa1070620:exon 30.0%
GTTGTTAGTGCTATGATTAT+TGG - chr7_2:66939676-66939695 Msa1070620:five_prime_UTR 30.0%
AGAAAGAGACAAAATGAAAC+AGG + chr7_2:66939732-66939751 None:intergenic 30.0%
!!! AACTTAGAGAGCACCAATTT+TGG - chr7_2:66938322-66938341 Msa1070620:three_prime_UTR 35.0%
AATTTGAACACACCAAGAGA+GGG + chr7_2:66938378-66938397 None:intergenic 35.0%
TAATTTGAACACACCAAGAG+AGG + chr7_2:66938379-66938398 None:intergenic 35.0%
AGGTGTAGAAAAAGTTAAGG+GGG + chr7_2:66938407-66938426 None:intergenic 35.0%
TTGAAAGGTTAGAGCAAGAA+GGG + chr7_2:66938496-66938515 None:intergenic 35.0%
CATAACTAGGAAGGACTTAT+AGG + chr7_2:66938651-66938670 None:intergenic 35.0%
GTTAGAACAAGGACATAACT+AGG + chr7_2:66938664-66938683 None:intergenic 35.0%
ATTATGTAGGAAGTGGATGT+TGG - chr7_2:66938864-66938883 Msa1070620:CDS 35.0%
! GGTAAAAGTACACAACTTTG+TGG + chr7_2:66938933-66938952 None:intergenic 35.0%
! AAGATGCTTAAAGGGTCTTT+TGG + chr7_2:66939101-66939120 None:intergenic 35.0%
!! TGACTCATTTTGAGGGTTTA+AGG + chr7_2:66939132-66939151 None:intergenic 35.0%
! GAACATAGTGACTCATTTTG+AGG + chr7_2:66939140-66939159 None:intergenic 35.0%
!!! AAGCTTGTTTTAAGAGAGGT+GGG + chr7_2:66939166-66939185 None:intergenic 35.0%
!!! TAAGCTTGTTTTAAGAGAGG+TGG + chr7_2:66939167-66939186 None:intergenic 35.0%
!! AGGAAAGGTTTGATTTTGAG+GGG - chr7_2:66939276-66939295 Msa1070620:intron 35.0%
GTCAAAATCCAATTCCAACA+AGG - chr7_2:66939321-66939340 Msa1070620:intron 35.0%
AAAATCCAATTCCAACAAGG+AGG - chr7_2:66939324-66939343 Msa1070620:intron 35.0%
GTACACATTGTTTGTCACAA+AGG - chr7_2:66939348-66939367 Msa1070620:intron 35.0%
ATGCATTGCATAGTGTCTTT+GGG + chr7_2:66939403-66939422 None:intergenic 35.0%
ACACTACTCACTAGGATTAA+TGG + chr7_2:66939561-66939580 None:intergenic 35.0%
TTAATCCTAGTGAGTAGTGT+TGG - chr7_2:66939561-66939580 Msa1070620:CDS 35.0%
!!! AACTTAGAGAGCACCAATTT+TGG - chr7_2:66938322-66938341 Msa1070620:three_prime_UTR 35.0%
AATTTGAACACACCAAGAGA+GGG + chr7_2:66938378-66938397 None:intergenic 35.0%
TAATTTGAACACACCAAGAG+AGG + chr7_2:66938379-66938398 None:intergenic 35.0%
AGGTGTAGAAAAAGTTAAGG+GGG + chr7_2:66938407-66938426 None:intergenic 35.0%
TTGAAAGGTTAGAGCAAGAA+GGG + chr7_2:66938496-66938515 None:intergenic 35.0%
CATAACTAGGAAGGACTTAT+AGG + chr7_2:66938651-66938670 None:intergenic 35.0%
GTTAGAACAAGGACATAACT+AGG + chr7_2:66938664-66938683 None:intergenic 35.0%
ATTATGTAGGAAGTGGATGT+TGG - chr7_2:66938864-66938883 Msa1070620:CDS 35.0%
! GGTAAAAGTACACAACTTTG+TGG + chr7_2:66938933-66938952 None:intergenic 35.0%
! AAGATGCTTAAAGGGTCTTT+TGG + chr7_2:66939101-66939120 None:intergenic 35.0%
!! TGACTCATTTTGAGGGTTTA+AGG + chr7_2:66939132-66939151 None:intergenic 35.0%
! GAACATAGTGACTCATTTTG+AGG + chr7_2:66939140-66939159 None:intergenic 35.0%
!!! AAGCTTGTTTTAAGAGAGGT+GGG + chr7_2:66939166-66939185 None:intergenic 35.0%
!!! TAAGCTTGTTTTAAGAGAGG+TGG + chr7_2:66939167-66939186 None:intergenic 35.0%
!! AGGAAAGGTTTGATTTTGAG+GGG - chr7_2:66939276-66939295 Msa1070620:intron 35.0%
GTCAAAATCCAATTCCAACA+AGG - chr7_2:66939321-66939340 Msa1070620:intron 35.0%
AAAATCCAATTCCAACAAGG+AGG - chr7_2:66939324-66939343 Msa1070620:intron 35.0%
GTACACATTGTTTGTCACAA+AGG - chr7_2:66939348-66939367 Msa1070620:intron 35.0%
ATGCATTGCATAGTGTCTTT+GGG + chr7_2:66939403-66939422 None:intergenic 35.0%
ACACTACTCACTAGGATTAA+TGG + chr7_2:66939561-66939580 None:intergenic 35.0%
TTAATCCTAGTGAGTAGTGT+TGG - chr7_2:66939561-66939580 Msa1070620:CDS 35.0%
ATTTGAACACACCAAGAGAG+GGG + chr7_2:66938377-66938396 None:intergenic 40.0%
GGTGTAGAAAAAGTTAAGGG+GGG + chr7_2:66938406-66938425 None:intergenic 40.0%
!! GGCACACATCCATTTTGATA+AGG + chr7_2:66938427-66938446 None:intergenic 40.0%
TAGTGAGTGCAAACAAGAGA+AGG - chr7_2:66938463-66938482 Msa1070620:three_prime_UTR 40.0%
CTTGAAAGGTTAGAGCAAGA+AGG + chr7_2:66938497-66938516 None:intergenic 40.0%
! CCTCTTTTCTGCTCAAAACA+CGG - chr7_2:66938529-66938548 Msa1070620:CDS 40.0%
GAACAAGGACATAACTAGGA+AGG + chr7_2:66938660-66938679 None:intergenic 40.0%
AAGTGGATGTTGGAATGGAA+TGG - chr7_2:66938874-66938893 Msa1070620:CDS 40.0%
GGTTTCTTCACTGTGTTTGA+AGG + chr7_2:66938996-66939015 None:intergenic 40.0%
!! AAAGGGTCTTTTGGTCTTCT+TGG + chr7_2:66939092-66939111 None:intergenic 40.0%
! GTTAGCTGATAGTGAACTCA+TGG - chr7_2:66939190-66939209 Msa1070620:CDS 40.0%
TAGTGAACTCATGGTACCTA+AGG - chr7_2:66939199-66939218 Msa1070620:CDS 40.0%
AGTGAACTCATGGTACCTAA+GGG - chr7_2:66939200-66939219 Msa1070620:CDS 40.0%
!! GTTACCTCTTTTTCACCCTT+AGG + chr7_2:66939218-66939237 None:intergenic 40.0%
!! GTTTGATTTTGAGGGGTTTG+AGG - chr7_2:66939283-66939302 Msa1070620:intron 40.0%
ATAGTGTCTTTGGGCCTAAT+GGG + chr7_2:66939394-66939413 None:intergenic 40.0%
CATGCATTGCATAGTGTCTT+TGG + chr7_2:66939404-66939423 None:intergenic 40.0%
AAGACACTATGCAATGCATG+TGG - chr7_2:66939404-66939423 Msa1070620:intron 40.0%
ACAAAAGTTGGACTCTTAGC+AGG + chr7_2:66939470-66939489 None:intergenic 40.0%
ACAAGAAAGTCATGGAGATG+AGG - chr7_2:66939513-66939532 Msa1070620:intron 40.0%
ATATGGTATGCAACATGAGG+AGG + chr7_2:66939755-66939774 None:intergenic 40.0%
GCAATATGGTATGCAACATG+AGG + chr7_2:66939758-66939777 None:intergenic 40.0%
ATTTGAACACACCAAGAGAG+GGG + chr7_2:66938377-66938396 None:intergenic 40.0%
GGTGTAGAAAAAGTTAAGGG+GGG + chr7_2:66938406-66938425 None:intergenic 40.0%
!! GGCACACATCCATTTTGATA+AGG + chr7_2:66938427-66938446 None:intergenic 40.0%
TAGTGAGTGCAAACAAGAGA+AGG - chr7_2:66938463-66938482 Msa1070620:three_prime_UTR 40.0%
CTTGAAAGGTTAGAGCAAGA+AGG + chr7_2:66938497-66938516 None:intergenic 40.0%
! CCTCTTTTCTGCTCAAAACA+CGG - chr7_2:66938529-66938548 Msa1070620:CDS 40.0%
GAACAAGGACATAACTAGGA+AGG + chr7_2:66938660-66938679 None:intergenic 40.0%
AAGTGGATGTTGGAATGGAA+TGG - chr7_2:66938874-66938893 Msa1070620:CDS 40.0%
GGTTTCTTCACTGTGTTTGA+AGG + chr7_2:66938996-66939015 None:intergenic 40.0%
!! AAAGGGTCTTTTGGTCTTCT+TGG + chr7_2:66939092-66939111 None:intergenic 40.0%
! GTTAGCTGATAGTGAACTCA+TGG - chr7_2:66939190-66939209 Msa1070620:CDS 40.0%
TAGTGAACTCATGGTACCTA+AGG - chr7_2:66939199-66939218 Msa1070620:CDS 40.0%
AGTGAACTCATGGTACCTAA+GGG - chr7_2:66939200-66939219 Msa1070620:CDS 40.0%
!! GTTACCTCTTTTTCACCCTT+AGG + chr7_2:66939218-66939237 None:intergenic 40.0%
!! GTTTGATTTTGAGGGGTTTG+AGG - chr7_2:66939283-66939302 Msa1070620:intron 40.0%
ATAGTGTCTTTGGGCCTAAT+GGG + chr7_2:66939394-66939413 None:intergenic 40.0%
CATGCATTGCATAGTGTCTT+TGG + chr7_2:66939404-66939423 None:intergenic 40.0%
AAGACACTATGCAATGCATG+TGG - chr7_2:66939404-66939423 Msa1070620:intron 40.0%
ACAAAAGTTGGACTCTTAGC+AGG + chr7_2:66939470-66939489 None:intergenic 40.0%
ACAAGAAAGTCATGGAGATG+AGG - chr7_2:66939513-66939532 Msa1070620:intron 40.0%
ATATGGTATGCAACATGAGG+AGG + chr7_2:66939755-66939774 None:intergenic 40.0%
GCAATATGGTATGCAACATG+AGG + chr7_2:66939758-66939777 None:intergenic 40.0%
CAAGAGAGGGGAAAATGGTA+AGG + chr7_2:66938365-66938384 None:intergenic 45.0%
! CTTACCATTTTCCCCTCTCT+TGG - chr7_2:66938363-66938382 Msa1070620:three_prime_UTR 45.0%
GGTCATCAAGGCTACTTGAA+AGG + chr7_2:66938511-66938530 None:intergenic 45.0%
GAGCAGAAAAGAGGTCATCA+AGG + chr7_2:66938523-66938542 None:intergenic 45.0%
!! CCGTGTTTTGAGCAGAAAAG+AGG + chr7_2:66938532-66938551 None:intergenic 45.0%
GTAGGAAGTGGATGTTGGAA+TGG - chr7_2:66938869-66938888 Msa1070620:CDS 45.0%
! GAGTCAACTTTTGCAGTTCC+AGG - chr7_2:66939032-66939051 Msa1070620:CDS 45.0%
! CTTTTGCTTCTTGCTTTGCC+TGG + chr7_2:66939053-66939072 None:intergenic 45.0%
CAGGCAAAGCAAGAAGCAAA+AGG - chr7_2:66939051-66939070 Msa1070620:CDS 45.0%
GGTACCTAAGGGTGAAAAAG+AGG - chr7_2:66939211-66939230 Msa1070620:intron 45.0%
GTACACCTCCTTGTTGGAAT+TGG + chr7_2:66939332-66939351 None:intergenic 45.0%
CAATGTGTACACCTCCTTGT+TGG + chr7_2:66939338-66939357 None:intergenic 45.0%
CATAGTGTCTTTGGGCCTAA+TGG + chr7_2:66939395-66939414 None:intergenic 45.0%
TATGCAATGCATGTGGTGTG+AGG - chr7_2:66939411-66939430 Msa1070620:intron 45.0%
TGTGGTGTGAGGTACAAATC+TGG - chr7_2:66939422-66939441 Msa1070620:intron 45.0%
GTGTGAGGTACAAATCTGGT+AGG - chr7_2:66939426-66939445 Msa1070620:intron 45.0%
CTCTTAGCAGGTCTATACTC+TGG + chr7_2:66939458-66939477 None:intergenic 45.0%
! ATCCTAGTGAGTAGTGTTGG+TGG - chr7_2:66939564-66939583 Msa1070620:CDS 45.0%
AACCACCAACACTACTCACT+AGG + chr7_2:66939569-66939588 None:intergenic 45.0%
CAAGAGAGGGGAAAATGGTA+AGG + chr7_2:66938365-66938384 None:intergenic 45.0%
! CTTACCATTTTCCCCTCTCT+TGG - chr7_2:66938363-66938382 Msa1070620:three_prime_UTR 45.0%
GGTCATCAAGGCTACTTGAA+AGG + chr7_2:66938511-66938530 None:intergenic 45.0%
GAGCAGAAAAGAGGTCATCA+AGG + chr7_2:66938523-66938542 None:intergenic 45.0%
!! CCGTGTTTTGAGCAGAAAAG+AGG + chr7_2:66938532-66938551 None:intergenic 45.0%
GTAGGAAGTGGATGTTGGAA+TGG - chr7_2:66938869-66938888 Msa1070620:CDS 45.0%
! GAGTCAACTTTTGCAGTTCC+AGG - chr7_2:66939032-66939051 Msa1070620:CDS 45.0%
! CTTTTGCTTCTTGCTTTGCC+TGG + chr7_2:66939053-66939072 None:intergenic 45.0%
CAGGCAAAGCAAGAAGCAAA+AGG - chr7_2:66939051-66939070 Msa1070620:CDS 45.0%
GGTACCTAAGGGTGAAAAAG+AGG - chr7_2:66939211-66939230 Msa1070620:intron 45.0%
GTACACCTCCTTGTTGGAAT+TGG + chr7_2:66939332-66939351 None:intergenic 45.0%
CAATGTGTACACCTCCTTGT+TGG + chr7_2:66939338-66939357 None:intergenic 45.0%
CATAGTGTCTTTGGGCCTAA+TGG + chr7_2:66939395-66939414 None:intergenic 45.0%
TATGCAATGCATGTGGTGTG+AGG - chr7_2:66939411-66939430 Msa1070620:intron 45.0%
TGTGGTGTGAGGTACAAATC+TGG - chr7_2:66939422-66939441 Msa1070620:intron 45.0%
GTGTGAGGTACAAATCTGGT+AGG - chr7_2:66939426-66939445 Msa1070620:intron 45.0%
CTCTTAGCAGGTCTATACTC+TGG + chr7_2:66939458-66939477 None:intergenic 45.0%
! ATCCTAGTGAGTAGTGTTGG+TGG - chr7_2:66939564-66939583 Msa1070620:CDS 45.0%
AACCACCAACACTACTCACT+AGG + chr7_2:66939569-66939588 None:intergenic 45.0%
CACACCAAGAGAGGGGAAAA+TGG + chr7_2:66938370-66938389 None:intergenic 50.0%
TGTCACAAAGGACCCCACAA+TGG - chr7_2:66939360-66939379 Msa1070620:intron 50.0%
ACAAAGGACCCCACAATGGA+GGG - chr7_2:66939364-66939383 Msa1070620:intron 50.0%
CACACCAAGAGAGGGGAAAA+TGG + chr7_2:66938370-66938389 None:intergenic 50.0%
TGTCACAAAGGACCCCACAA+TGG - chr7_2:66939360-66939379 Msa1070620:intron 50.0%
ACAAAGGACCCCACAATGGA+GGG - chr7_2:66939364-66939383 Msa1070620:intron 50.0%
CACAAAGGACCCCACAATGG+AGG - chr7_2:66939363-66939382 Msa1070620:intron 55.0%
CACAAAGGACCCCACAATGG+AGG - chr7_2:66939363-66939382 Msa1070620:intron 55.0%
ATGGGCCTGCCCTCCATTGT+GGG + chr7_2:66939376-66939395 None:intergenic 60.0%
AATGGGCCTGCCCTCCATTG+TGG + chr7_2:66939377-66939396 None:intergenic 60.0%
CAATGGAGGGCAGGCCCATT+AGG - chr7_2:66939377-66939396 Msa1070620:intron 60.0%
ATGGGCCTGCCCTCCATTGT+GGG + chr7_2:66939376-66939395 None:intergenic 60.0%
AATGGGCCTGCCCTCCATTG+TGG + chr7_2:66939377-66939396 None:intergenic 60.0%
CAATGGAGGGCAGGCCCATT+AGG - chr7_2:66939377-66939396 Msa1070620:intron 60.0%
AGGACCCCACAATGGAGGGC+AGG - chr7_2:66939368-66939387 Msa1070620:intron 65.0%
TGGGCCTGCCCTCCATTGTG+GGG + chr7_2:66939375-66939394 None:intergenic 65.0%
AGGACCCCACAATGGAGGGC+AGG - chr7_2:66939368-66939387 Msa1070620:intron 65.0%
TGGGCCTGCCCTCCATTGTG+GGG + chr7_2:66939375-66939394 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 66938296 66939802 66938296 ID=Msa1070620;Name=Msa1070620
chr7_2 mRNA 66938296 66939802 66938296 ID=Msa1070620-mRNA-1;Parent=Msa1070620;Name=Msa1070620-mRNA-1;_AED=0.01;_eAED=0.01;_QI=138|1|1|1|1|1|2|225|273
chr7_2 exon 66938296 66939225 66938296 ID=Msa1070620-mRNA-1:exon:14399;Parent=Msa1070620-mRNA-1
chr7_2 exon 66939548 66939802 66939548 ID=Msa1070620-mRNA-1:exon:14398;Parent=Msa1070620-mRNA-1
chr7_2 five_prime_UTR 66939665 66939802 66939665 ID=Msa1070620-mRNA-1:five_prime_utr;Parent=Msa1070620-mRNA-1
chr7_2 CDS 66939548 66939664 66939548 ID=Msa1070620-mRNA-1:cds;Parent=Msa1070620-mRNA-1
chr7_2 CDS 66938521 66939225 66938521 ID=Msa1070620-mRNA-1:cds;Parent=Msa1070620-mRNA-1
chr7_2 three_prime_UTR 66938296 66938520 66938296 ID=Msa1070620-mRNA-1:three_prime_utr;Parent=Msa1070620-mRNA-1
Gene Sequence

>Msa1070620

ATGGATGTGTGCCAAAATGTACAAGTTTCTAGTGAGTGCAAACAAGAGAAGGTTCTTAACCCTTCTTGCTCTAACCTTTCAAGTAGCCTTGATGACCTCTTTTCTGCTCAAAACACGGAAGTGGATGTTGGAATGGAATGGCTTTCAGTGTTTGTTGAAGACTGTTTTTCAAGCCCACAAAGTTGTGTACTTTTACCCTCTAATGTTCAAAACACAACAAGTACTGTCTCATCAAAACCTTCAAACACAGTGAAGAAACCAAAACAAGAACAAAATGAGTCAACTTTTGCAGTTCCAGGCAAAGCAAGAAGCAAAAGGAAGAGACTTTCAGCTCCAAGAAGACCAAAAGACCCTTTAAGCATCTTATCCAATACCTTAAACCCTCAAAATGAGTCACTATGTTCTGACCCACCTCTCTTAAAACAAGCTTATTGGTTAGCTGATAGTGAACTCATGGTACCTAAGGGTGAAAAAGAGGTAACAAAAGATTATGAAGTGGTTGAAAAGGAAATTGTGAAAAAGGAAAGGTTTGATTTTGAGGGGTTTGAGGTTGATGTTAACAATGGTCAAAATCCAATTCCAACAAGGAGGTGTACACATTGTTTGTCACAAAGGACCCCACAATGGAGGGCAGGCCCATTAGGCCCAAAGACACTATGCAATGCATGTGGTGTGAGGTACAAATCTGGTAGGTTGTTGCCAGAGTATAGACCTGCTAAGAGTCCAACTTTTGTGAGTTTTTTGCATTCAAATTCACACAAGAAAGTCATGGAGATGAGGATGAATGTGAATGTTGTATCTTCCATTAATCCTAGTGAGTAG
Protein sequence

>Msa1070620

MDVCQNVQVSSECKQEKVLNPSCSNLSSSLDDLFSAQNTEVDVGMEWLSVFVEDCFSSPQSCVLLPSNVQNTTSTVSSKPSNTVKKPKQEQNESTFAVPGKARSKRKRLSAPRRPKDPLSILSNTLNPQNESLCSDPPLLKQAYWLADSELMVPKGEKEVTKDYEVVEKEIVKKERFDFEGFEVDVNNGQNPIPTRRCTHCLSQRTPQWRAGPLGPKTLCNACGVRYKSGRLLPEYRPAKSPTFVSFLHSNSHKKVMEMRMNVNVVSSINPSE*