AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0687090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0687090 MtrunA17_Chr4g0037331 96.839 348 7 1 1 348 1 344 0.0 665
Msa0687090 MtrunA17_Chr5g0405611 67.917 240 72 2 11 248 80 316 2.43e-117 344
Msa0687090 MtrunA17_Chr8g0371771 62.963 270 94 3 14 280 18 284 2.48e-114 334
Msa0687090 MtrunA17_Chr1g0156711 61.977 263 88 6 8 258 3 265 1.50e-104 310
Msa0687090 MtrunA17_Chr3g0142781 53.398 309 125 9 1 296 1 303 1.54e-99 297
Msa0687090 MtrunA17_Chr8g0352471 52.703 296 125 6 9 289 9 304 2.88e-97 291
Msa0687090 MtrunA17_Chr8g0371781 60.996 241 82 4 20 249 23 262 2.11e-95 286
Msa0687090 MtrunA17_Chr5g0405581 54.815 270 110 5 9 266 9 278 2.08e-92 278
Msa0687090 MtrunA17_Chr4g0037351 55.285 246 100 4 3 240 5 248 1.00e-89 272
Msa0687090 MtrunA17_Chr8g0357311 55.777 251 101 6 4 246 7 255 4.60e-89 268
Msa0687090 MtrunA17_Chr6g0482741 52.479 242 108 4 4 240 1 240 7.47e-88 264
Msa0687090 MtrunA17_Chr5g0428801 53.815 249 100 5 4 242 9 252 1.09e-84 259
Msa0687090 MtrunA17_Chr3g0141341 53.165 237 103 5 14 244 1 235 2.13e-84 255
Msa0687090 MtrunA17_Chr8g0351851 53.333 240 105 4 4 239 1 237 2.18e-84 255
Msa0687090 MtrunA17_Chr1g0154591 54.661 236 99 4 15 244 3 236 9.15e-84 253
Msa0687090 MtrunA17_Chr8g0392961 50.612 245 114 4 3 242 5 247 1.29e-82 251
Msa0687090 MtrunA17_Chr7g0265011 49.597 248 118 5 1 242 43 289 5.54e-81 248
Msa0687090 MtrunA17_Chr1g0180121 53.252 246 102 7 4 240 1 242 1.63e-79 243
Msa0687090 MtrunA17_Chr1g0180161 52.419 248 105 7 4 242 1 244 3.59e-79 243
Msa0687090 MtrunA17_Chr1g0180221 52.209 249 110 7 1 242 38 284 6.21e-77 238
Msa0687090 MtrunA17_Chr1g0180231 52.016 248 106 7 4 242 1 244 2.88e-76 234
Msa0687090 MtrunA17_Chr1g0179871 52.016 248 106 7 4 242 1 244 2.88e-76 234
Msa0687090 MtrunA17_Chr1g0179851 52.066 242 108 6 7 242 2 241 5.64e-76 234
Msa0687090 MtrunA17_Chr2g0312181 50.833 240 108 5 8 240 7 243 7.23e-76 233
Msa0687090 MtrunA17_Chr1g0179891 54.185 227 97 5 22 242 14 239 1.48e-75 233
Msa0687090 MtrunA17_Chr5g0424221 48.000 250 119 7 23 269 27 268 3.18e-72 226
Msa0687090 MtrunA17_Chr2g0311561 49.351 231 111 4 21 245 7 237 2.45e-70 221
Msa0687090 MtrunA17_Chr3g0112091 45.570 237 121 5 11 240 2 237 9.38e-70 218
Msa0687090 MtrunA17_Chr2g0311521 50.218 229 106 4 22 242 21 249 1.62e-68 231
Msa0687090 MtrunA17_Chr7g0239131 45.378 238 120 4 8 240 10 242 3.73e-66 209
Msa0687090 MtrunA17_Chr2g0311551 48.889 225 109 4 22 240 20 244 1.38e-65 223
Msa0687090 MtrunA17_Chr6g0452331 43.701 254 129 8 1 241 1 253 1.93e-64 205
Msa0687090 MtrunA17_Chr4g0031221 45.798 238 116 8 13 239 16 251 7.69e-63 201
Msa0687090 MtrunA17_Chr5g0397821 46.575 219 97 10 25 240 24 225 7.91e-50 167
Msa0687090 MtrunA17_Chr2g0311531 42.291 227 101 8 21 242 23 224 1.15e-47 161
Msa0687090 MtrunA17_Chr8g0386341 41.453 234 114 10 11 240 34 248 1.48e-46 158
Msa0687090 MtrunA17_Chr5g0406771 48.864 176 79 6 12 179 3 175 1.81e-46 155
Msa0687090 MtrunA17_Chr5g0397831 38.800 250 129 10 1 240 1 236 5.06e-45 154
Msa0687090 MtrunA17_Chr2g0311501 41.622 185 99 5 66 242 1 184 8.77e-42 144
Msa0687090 MtrunA17_Chr2g0309611 30.705 241 148 7 3 229 4 239 5.68e-34 125
Msa0687090 MtrunA17_Chr1g0179911 30.769 247 149 7 6 241 4 239 7.58e-31 117
Msa0687090 MtrunA17_Chr8g0386351 44.304 158 73 8 9 164 48 192 1.64e-26 105
Msa0687090 MtrunA17_Chr1g0180181 53.571 84 34 2 164 242 18 101 2.20e-20 85.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0687090 AT4G38660.1 63.636 352 101 6 5 345 9 344 8.11e-144 410
Msa0687090 AT4G38660.2 64.583 336 93 5 20 345 3 322 1.87e-140 401
Msa0687090 AT4G24180.2 68.651 252 69 4 5 248 10 259 5.11e-121 351
Msa0687090 AT4G24180.1 67.843 255 71 4 5 248 10 264 1.01e-120 351
Msa0687090 AT4G24180.5 68.826 247 67 4 5 243 10 254 5.30e-119 344
Msa0687090 AT4G24180.6 68.000 250 69 4 5 243 10 259 8.57e-119 344
Msa0687090 AT4G24180.4 67.249 229 65 4 5 225 10 236 1.57e-106 315
Msa0687090 AT4G24180.3 66.379 232 67 4 5 225 10 241 2.30e-106 315
Msa0687090 AT1G75800.1 51.911 314 132 7 1 303 1 306 1.72e-97 292
Msa0687090 AT1G20030.2 64.502 231 72 5 21 241 16 246 8.26e-96 287
Msa0687090 AT1G20030.1 65.487 226 68 5 26 241 4 229 5.31e-95 285
Msa0687090 AT4G36010.1 58.468 248 90 5 21 255 19 266 9.28e-93 279
Msa0687090 AT4G36010.2 58.468 248 90 5 21 255 19 266 9.28e-93 279
Msa0687090 AT1G19320.1 56.048 248 98 5 1 239 1 246 6.27e-91 273
Msa0687090 AT2G17860.1 54.688 256 102 6 1 245 1 253 5.45e-89 268
Msa0687090 AT1G75030.1 57.200 250 92 8 1 241 1 244 6.82e-89 267
Msa0687090 AT1G75040.1 56.148 244 98 6 1 240 1 239 4.34e-86 260
Msa0687090 AT1G75050.1 57.025 242 91 7 9 241 7 244 5.78e-86 260
Msa0687090 AT1G18250.1 56.000 225 92 4 23 242 19 241 3.11e-85 258
Msa0687090 AT1G18250.2 56.502 223 90 4 25 242 22 242 4.41e-85 258
Msa0687090 AT1G73620.1 56.000 225 92 4 23 242 40 262 3.56e-84 256
Msa0687090 AT4G38670.3 55.556 243 97 6 9 240 5 247 4.40e-83 254
Msa0687090 AT4G38670.1 55.556 243 97 6 9 240 5 247 4.40e-83 254
Msa0687090 AT4G38670.2 58.482 224 84 5 26 240 8 231 1.03e-82 252
Msa0687090 AT5G24620.1 52.419 248 102 6 25 257 25 271 1.51e-81 254
Msa0687090 AT5G24620.4 52.419 248 102 6 25 257 80 326 1.80e-81 256
Msa0687090 AT5G24620.3 52.419 248 102 6 25 257 101 347 3.29e-81 256
Msa0687090 AT4G36000.2 54.472 246 68 7 101 345 1 203 1.63e-76 234
Msa0687090 AT4G36000.1 54.472 246 68 7 101 345 1 203 1.63e-76 234
Msa0687090 AT1G77700.3 50.847 236 101 7 14 241 45 273 3.80e-74 233
Msa0687090 AT1G77700.2 49.587 242 111 7 4 241 39 273 8.09e-74 231
Msa0687090 AT1G77700.1 51.351 222 98 6 23 241 87 301 1.93e-73 231
Msa0687090 AT5G38280.1 45.736 258 119 6 12 248 7 264 2.02e-72 237
Msa0687090 AT5G38280.2 47.131 244 113 5 21 248 21 264 4.70e-72 236
Msa0687090 AT5G40020.1 43.426 251 132 4 1 242 1 250 9.11e-68 214
Msa0687090 AT2G28790.1 47.755 245 115 8 4 239 8 248 2.56e-65 207
Msa0687090 AT2G28790.2 47.755 245 115 8 4 239 33 273 2.61e-65 208
Msa0687090 AT5G02140.1 45.992 237 121 5 10 240 6 241 3.49e-64 206
Msa0687090 AT5G02140.2 45.992 237 121 5 10 240 6 241 4.66e-64 204
Msa0687090 AT4G18253.1 39.834 241 136 3 9 245 8 243 1.12e-54 179
Msa0687090 AT4G11650.1 43.846 260 122 12 2 257 3 242 8.91e-53 175
Msa0687090 AT1G70250.1 39.764 254 130 8 26 266 146 389 1.29e-51 183
Msa0687090 AT4G18250.1 40.506 237 132 5 23 254 55 287 2.50e-44 162
Msa0687090 AT5G24620.2 51.429 140 55 4 131 257 15 154 5.33e-39 140
Msa0687090 AT2G24810.1 61.039 77 27 1 166 239 116 192 1.04e-25 102

Find 162 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGGTTGGTCCGGCCGGTTC+TGG 0.121811 4_4:-51823298 Msa0687090:CDS
TCTGCATTTGTTTACTCTTT+TGG 0.167211 4_4:-51821872 Msa0687090:CDS
CCAACATATTTCTCCAATTT+CGG 0.184894 4_4:-51821520 Msa0687090:three_prime_UTR
CAACATATTTCTCCAATTTC+GGG 0.194131 4_4:-51821519 Msa0687090:three_prime_UTR
TTTGAGGTTCTATGATTGTT+AGG 0.205309 4_4:-51821556 Msa0687090:three_prime_UTR
GAAATGAAATGAGGGAACTA+TGG 0.253373 4_4:+51823911 None:intergenic
GTGAAGTGAGTGAGGAGTTT+AGG 0.274623 4_4:+51823793 None:intergenic
CAACAAAGATAGCCCGAAAT+TGG 0.302284 4_4:+51821507 None:intergenic
TTTGTTAAGTGAAATGTAAA+TGG 0.311452 4_4:-51821775 Msa0687090:three_prime_UTR
CAATTTCGGGCTATCTTTGT+TGG 0.317702 4_4:-51821506 Msa0687090:three_prime_UTR
TATTCAGTTCCCGTAAATTC+AGG 0.318296 4_4:-51821998 Msa0687090:CDS
TGAGGGAACTATGGGGCTAT+TGG 0.330284 4_4:+51823920 None:intergenic
GTGAACTCAGCTAGGGTTGC+TGG 0.336395 4_4:+51823173 None:intergenic
CCAGGAGCAGGGACAGTGAT+AGG 0.340612 4_4:-51822088 Msa0687090:CDS
TGATCCGGACCCTGAATTTA+CGG 0.349274 4_4:+51821988 None:intergenic
TTGAGGTTCTATGATTGTTA+GGG 0.350769 4_4:-51821555 Msa0687090:three_prime_UTR
GCTATTGGATGCCCATGACC+TGG 0.352249 4_4:+51823935 None:intergenic
ACTGTCCCTGCTCCTGGTAT+TGG 0.357647 4_4:+51822094 None:intergenic
CCCATGATTGTTGAAGGTTC+TGG 0.359030 4_4:-51823092 Msa0687090:CDS
CGGTTGGTCCGGCCGGTTCT+GGG 0.371851 4_4:-51823297 Msa0687090:CDS
CCAGAACCGGCCGGACCAAC+CGG 0.372349 4_4:+51823298 None:intergenic
GGTATTGGTGTTGAGGTCAT+TGG 0.375177 4_4:+51822109 None:intergenic
GTTGAGGTCATTGGAGTTGT+TGG 0.375805 4_4:+51822118 None:intergenic
TCAACTCCGCCGGACATTGC+TGG 0.377638 4_4:+51823024 None:intergenic
TAAGCGTAGCTATACGATCT+AGG 0.389947 4_4:+51822867 None:intergenic
TCCGGATCAGCATCTAATTC+AGG 0.394047 4_4:-51821974 Msa0687090:CDS
CTCCAGCCGGTTGGTCCGGC+CGG 0.396644 4_4:-51823304 Msa0687090:CDS
TTTAGGAAGGAGAGTGTTGT+TGG 0.400569 4_4:+51823810 None:intergenic
TACGACGTGAGTCTCGTTGA+TGG 0.402827 4_4:-51823125 Msa0687090:CDS
AAGGTGGGTGGTTTACAATA+AGG 0.407513 4_4:+51821679 None:intergenic
GTTGGAACAGGATCTGAAAA+TGG 0.408945 4_4:-51822055 Msa0687090:CDS
GATCCGGACCCTGAATTTAC+GGG 0.410876 4_4:+51821989 None:intergenic
CAAGCTCCAGCCGGTTGGTC+CGG 0.411921 4_4:-51823308 Msa0687090:CDS
GGCCGGTTCTGGGCTCGAAC+CGG 0.412457 4_4:-51823287 Msa0687090:CDS
AAGAGCGCGTGTGAGGCTTT+TGG 0.414184 4_4:-51822978 Msa0687090:CDS
AAACCGGTGCTTCCGAGTGT+TGG 0.422655 4_4:+51823362 None:intergenic
ACAAATGTGCCTACACAGTA+TGG 0.422746 4_4:-51823412 Msa0687090:CDS
TCAGATCCTGTTCCAACCTC+AGG 0.424740 4_4:+51822061 None:intergenic
AACCGGTGCTTCCGAGTGTT+GGG 0.429937 4_4:+51823363 None:intergenic
ACCAGAACCTTCAACAATCA+TGG 0.434467 4_4:+51823091 None:intergenic
ATGGTTCACCGGCTACTTGT+AGG 0.436214 4_4:-51822926 Msa0687090:CDS
TCGGGTATGTGTGAGTCCAC+CGG 0.437031 4_4:-51823065 Msa0687090:CDS
AATCTACCCATGATTGTTGA+AGG 0.439747 4_4:-51823098 Msa0687090:CDS
GGCTTGTTGCTCACTAAAGC+TGG 0.439918 4_4:-51821714 Msa0687090:three_prime_UTR
AACCGGTTCGAGCCCAGAAC+CGG 0.440678 4_4:+51823285 None:intergenic
GTTGAAGGTTCTGGTGGGTC+GGG 0.448370 4_4:-51823083 Msa0687090:CDS
CCAGCATTTGCGAGAATACC+TGG 0.448703 4_4:+51823389 None:intergenic
TTCTTGTGGAAGCTCGAAAC+CGG 0.455126 4_4:+51823346 None:intergenic
CCTATCACTGTCCCTGCTCC+TGG 0.456736 4_4:+51822088 None:intergenic
GAGTTCACTCTTGGCACTAT+AGG 0.458610 4_4:-51823158 Msa0687090:CDS
TGGATCATATTTAGCTGGAT+TGG 0.461151 4_4:-51821924 Msa0687090:CDS
AACCGGCCGGACCAACCGGC+TGG 0.468762 4_4:+51823302 None:intergenic
TTGTAGGCCGTCGATTTACT+CGG 0.469663 4_4:-51822910 Msa0687090:CDS
CGGGTATGTGTGAGTCCACC+GGG 0.470901 4_4:-51823064 Msa0687090:CDS
TCAGCATCTAATTCAGGATC+AGG 0.473729 4_4:-51821968 Msa0687090:CDS
GGTTGCTGGTGGTGCAGCTC+CGG 0.475246 4_4:+51823187 None:intergenic
GGTTGCAACTTCGACGGATC+CGG 0.476364 4_4:-51823266 Msa0687090:CDS
TTAGGAAGGAGAGTGTTGTT+GGG 0.477017 4_4:+51823811 None:intergenic
CCACCCACCTTAAGATTCGT+TGG 0.479262 4_4:-51821667 Msa0687090:three_prime_UTR
GGGACTCCTGAGGTTGGAAC+AGG 0.482015 4_4:-51822067 Msa0687090:CDS
CCTTGGAGAAGATGGACAAA+AGG 0.482274 4_4:+51822794 None:intergenic
TGTTGAAGGTTCTGGTGGGT+CGG 0.482817 4_4:-51823084 Msa0687090:CDS
TTGTTAAGTGAAATGTAAAT+GGG 0.483898 4_4:-51821774 Msa0687090:three_prime_UTR
GCAGATGGATCATATTTAGC+TGG 0.484088 4_4:-51821929 Msa0687090:CDS
CGCACTTTCCAAGCTCCAGC+CGG 0.485696 4_4:-51823317 Msa0687090:CDS
GTTCCCGTAAATTCAGGGTC+CGG 0.490170 4_4:-51821992 Msa0687090:CDS
CGGACCAACCGGCTGGAGCT+TGG 0.491158 4_4:+51823309 None:intergenic
AAATGAAATGAGGGAACTAT+GGG 0.491443 4_4:+51823912 None:intergenic
TCCTGAATTAGATGCTGATC+CGG 0.492799 4_4:+51821973 None:intergenic
ATTGTGTTGTGGAATGGTGG+TGG 0.495582 4_4:+51823754 None:intergenic
CACCCACCTTAAGATTCGTT+GGG 0.495910 4_4:-51821666 Msa0687090:three_prime_UTR
TAACCTAGTTAGTTGTTGCT+TGG 0.496269 4_4:+51823680 None:intergenic
TGAGTGGAGTTAGCAAGAAA+AGG 0.498650 4_4:+51823843 None:intergenic
CCGAAATTGGAGAAATATGT+TGG 0.499604 4_4:+51821520 None:intergenic
CATGTTTGAGCCACAGTCTC+CGG 0.501661 4_4:+51823226 None:intergenic
GGATCTGAACCAGCAATGTC+CGG 0.501693 4_4:-51823033 Msa0687090:CDS
TAATCCGTCGACCCAGGTCA+TGG 0.502624 4_4:-51823947 Msa0687090:five_prime_UTR
TGCTCCTGGTATTGGTGTTG+AGG 0.514274 4_4:+51822102 None:intergenic
GGTGAGACATGAACCGGTGC+CGG 0.522698 4_4:+51823247 None:intergenic
ATTCAGTTCCCGTAAATTCA+GGG 0.523849 4_4:-51821997 Msa0687090:CDS
ACCCTAGCTGAGTTCACTCT+TGG 0.527571 4_4:-51823167 Msa0687090:CDS
AACTTCGACGGATCCGGCAC+CGG 0.529368 4_4:-51823260 Msa0687090:CDS
CCAGGTATTCTCGCAAATGC+TGG 0.530746 4_4:-51823389 Msa0687090:CDS
AGTGAGTGAGGAGTTTAGGA+AGG 0.533192 4_4:+51823797 None:intergenic
ACGGGTACAGACACACCCAT+TGG 0.533892 4_4:-51822022 Msa0687090:CDS
ATGGTAGAATGCAACGGTGC+CGG 0.536138 4_4:-51823206 Msa0687090:CDS
CGGGTACAGACACACCCATT+GGG 0.537883 4_4:-51822021 Msa0687090:CDS
GTCTCCGGTGAGACATGAAC+CGG 0.538568 4_4:+51823241 None:intergenic
GGATCCGTCGAAGTTGCAAC+CGG 0.539477 4_4:+51823268 None:intergenic
AAACCAAGCAACAACTAACT+AGG 0.540465 4_4:-51823683 Msa0687090:five_prime_UTR
GTGATAGGGACTCCTGAGGT+TGG 0.541020 4_4:-51822073 Msa0687090:CDS
ACGGGAACTGAATACCCAAT+GGG 0.541906 4_4:+51822007 None:intergenic
AAGCGTAGCTATACGATCTA+GGG 0.544232 4_4:+51822868 None:intergenic
AGCCCAACACTCGGAAGCAC+CGG 0.545623 4_4:-51823365 Msa0687090:CDS
GTTCAGATCCGACGTGCACC+CGG 0.545857 4_4:+51823046 None:intergenic
CTTTCCAAGCTCCAGCCGGT+TGG 0.546315 4_4:-51823313 Msa0687090:CDS
CTATTGGATGCCCATGACCT+GGG 0.549052 4_4:+51823936 None:intergenic
AAAGAGAGTGATATAAGTTG+AGG 0.549260 4_4:+51823584 None:intergenic
GGAGAAACCATGCTTGCAGA+TGG 0.549482 4_4:-51821944 Msa0687090:CDS
TGTTGGGATTTGATAATGAG+TGG 0.550208 4_4:+51823827 None:intergenic
TGAGGTTCTATGATTGTTAG+GGG 0.550522 4_4:-51821554 Msa0687090:three_prime_UTR
TATTGTTGTAGTGGCGCGTA+TGG 0.551438 4_4:-51822945 Msa0687090:CDS
ATGATTGTGTTGTGGAATGG+TGG 0.552659 4_4:+51823751 None:intergenic
AATGAAATGAAATGAAATGA+GGG 0.552703 4_4:+51823903 None:intergenic
CGCGTGTAAGAGCGCGTGTG+AGG 0.556474 4_4:-51822985 Msa0687090:CDS
TGCCAAGAGTGAACTCAGCT+AGG 0.556861 4_4:+51823165 None:intergenic
ATGATTGTTGAAGGTTCTGG+TGG 0.557532 4_4:-51823089 Msa0687090:CDS
ATGCCCATGACCTGGGTCGA+CGG 0.558345 4_4:+51823943 None:intergenic
TCAACACCAATACCAGGAGC+AGG 0.558407 4_4:-51822100 Msa0687090:CDS
CAATGAAATGAAATGAAATG+AGG 0.561656 4_4:+51823902 None:intergenic
ATATGATCCATCTGCAAGCA+TGG 0.561786 4_4:+51821937 None:intergenic
GCCAAGAGTGAACTCAGCTA+GGG 0.562984 4_4:+51823166 None:intergenic
TATTTAGCTGGATTGGCCAT+GGG 0.568640 4_4:-51821917 Msa0687090:CDS
TTTCCCAACGAATCTTAAGG+TGG 0.568778 4_4:+51821663 None:intergenic
AATCCGTCGACCCAGGTCAT+GGG 0.568964 4_4:-51823946 Msa0687090:five_prime_UTR
CAGGAGCAGGGACAGTGATA+GGG 0.571920 4_4:-51822087 Msa0687090:CDS
TCTGAAGCTCTCAACTCCGC+CGG 0.574441 4_4:+51823014 None:intergenic
CGGAGACTGTGGCTCAAACA+TGG 0.576529 4_4:-51823225 Msa0687090:CDS
CCAACGAATCTTAAGGTGGG+TGG 0.577670 4_4:+51821667 None:intergenic
TGTGCCTACACAGTATGGCC+AGG 0.579329 4_4:-51823407 Msa0687090:CDS
AACATTTCCGAGTAAATCGA+CGG 0.580067 4_4:+51822903 None:intergenic
GAGGTTCTATGATTGTTAGG+GGG 0.582819 4_4:-51821553 Msa0687090:three_prime_UTR
TAGTGGCGCGTATGGTTCAC+CGG 0.583066 4_4:-51822937 Msa0687090:CDS
ATGATGATTGTGTTGTGGAA+TGG 0.583728 4_4:+51823748 None:intergenic
CCAGAACCTTCAACAATCAT+GGG 0.586909 4_4:+51823092 None:intergenic
TGCAGATGAAGTTGTTGTAG+AGG 0.589695 4_4:+51821889 None:intergenic
ATATTTAGCTGGATTGGCCA+TGG 0.590981 4_4:-51821918 Msa0687090:CDS
AACCTTTAACGAATAAAAGA+CGG 0.593371 4_4:+51821634 None:intergenic
TCATGTCTCACCGGAGACTG+TGG 0.594100 4_4:-51823236 Msa0687090:CDS
ATTTATTAATCCGTCGACCC+AGG 0.594647 4_4:-51823953 Msa0687090:five_prime_UTR
TACGGGAACTGAATACCCAA+TGG 0.595273 4_4:+51822006 None:intergenic
TTCCCAACGAATCTTAAGGT+GGG 0.596443 4_4:+51821664 None:intergenic
GCGGAGTTGAGAGCTTCAGA+TGG 0.605619 4_4:-51823011 Msa0687090:CDS
TGAATATGATGATTGTGTTG+TGG 0.606360 4_4:+51823743 None:intergenic
TTAGCTGGATTGGCCATGGG+AGG 0.610887 4_4:-51821914 Msa0687090:CDS
GGTTCGAGCCCAGAACCGGC+CGG 0.616663 4_4:+51823289 None:intergenic
ATGACCTCAACACCAATACC+AGG 0.616871 4_4:-51822106 Msa0687090:CDS
TGTTGTAGAGGAGCCTCCCA+TGG 0.618276 4_4:+51821901 None:intergenic
AGTAGTTTACATTACACACA+TGG 0.620193 4_4:-51823616 Msa0687090:exon
AGTCCTGAGTATTGTTGTAG+TGG 0.630959 4_4:-51822954 Msa0687090:CDS
AATGCTGGTAGCCCAACACT+CGG 0.633231 4_4:-51823374 Msa0687090:CDS
CGAACCGGTTGCAACTTCGA+CGG 0.637688 4_4:-51823272 Msa0687090:CDS
GGCACCGGTTCATGTCTCAC+CGG 0.639118 4_4:-51823245 Msa0687090:CDS
TGAGTCCACCGGGTGCACGT+CGG 0.640829 4_4:-51823054 Msa0687090:CDS
GCATCTAATTCAGGATCAGG+AGG 0.640976 4_4:-51821965 Msa0687090:CDS
TGGAGCTTGGAAAGTGCGAG+TGG 0.641087 4_4:+51823322 None:intergenic
ATCGACGGCCTACAAGTAGC+CGG 0.644323 4_4:+51822918 None:intergenic
GCGCCACTACAACAATACTC+AGG 0.648242 4_4:+51822951 None:intergenic
AATACCTGGCCATACTGTGT+AGG 0.651875 4_4:+51823403 None:intergenic
TCAAACATGGTAGAATGCAA+CGG 0.654931 4_4:-51823212 Msa0687090:CDS
AGAAGATGGACAAAAGGTAA+CGG 0.655103 4_4:+51822800 None:intergenic
AACTCAGCTAGGGTTGCTGG+TGG 0.659719 4_4:+51823176 None:intergenic
TGATTGTTGAAGGTTCTGGT+GGG 0.661500 4_4:-51823088 Msa0687090:CDS
CAACACCAATACCAGGAGCA+GGG 0.663691 4_4:-51822099 Msa0687090:CDS
TGAAAATGGAGTTCAATACA+CGG 0.669710 4_4:-51822041 Msa0687090:CDS
AAAGTGCGAGTGGTTTCTTG+TGG 0.674516 4_4:+51823332 None:intergenic
GAAAATGGAGTTCAATACAC+GGG 0.685341 4_4:-51822040 Msa0687090:CDS
TGTTACAAGTGAAGTGAGTG+AGG 0.689694 4_4:+51823785 None:intergenic
CAGATCCGACGTGCACCCGG+TGG 0.699020 4_4:+51823049 None:intergenic
GACAGTGATAGGGACTCCTG+AGG 0.705413 4_4:-51822077 Msa0687090:CDS
TCTGAACCAGCAATGTCCGG+CGG 0.706133 4_4:-51823030 Msa0687090:CDS
AATGAAATGAGGGAACTATG+GGG 0.733493 4_4:+51823913 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTAAAACATAGACAA+AGG + chr4_4:51821948-51821967 None:intergenic 15.0%
!! ATTAATAGTAGATTTATTGT+TGG - chr4_4:51823054-51823073 Msa0687090:CDS 15.0%
!!! TGTTTTATTAGTATTAATCT+TGG - chr4_4:51823865-51823884 Msa0687090:five_prime_UTR 15.0%
!!! GTTTTATTAGTATTAATCTT+GGG - chr4_4:51823866-51823885 Msa0687090:five_prime_UTR 15.0%
!! AATGAAATGAAATGAAATGA+GGG + chr4_4:51821569-51821588 None:intergenic 20.0%
!!! TTACTTTTTTTGTTTGTCTT+AGG + chr4_4:51821826-51821845 None:intergenic 20.0%
!!! TTTTTAACATGTTTTTGTTC+AGG - chr4_4:51822005-51822024 Msa0687090:CDS 20.0%
!!! ATGTTAAAAGTATGTTGATT+AGG - chr4_4:51822728-51822747 Msa0687090:intron 20.0%
!!! TGTTAAAAGTATGTTGATTA+GGG - chr4_4:51822729-51822748 Msa0687090:intron 20.0%
!!! AATTTTGTGATTGTAGAAAT+TGG - chr4_4:51823276-51823295 Msa0687090:CDS 20.0%
!! TTTGTTAAGTGAAATGTAAA+TGG - chr4_4:51823694-51823713 Msa0687090:five_prime_UTR 20.0%
!! TTGTTAAGTGAAATGTAAAT+GGG - chr4_4:51823695-51823714 Msa0687090:five_prime_UTR 20.0%
! CAATGAAATGAAATGAAATG+AGG + chr4_4:51821570-51821589 None:intergenic 25.0%
!!! AGTGTTGATTAATTTTACCT+TGG + chr4_4:51822695-51822714 None:intergenic 25.0%
! TAACAACATTGTCGTATAAT+GGG + chr4_4:51822756-51822775 None:intergenic 25.0%
! TTAACAACATTGTCGTATAA+TGG + chr4_4:51822757-51822776 None:intergenic 25.0%
! GTCAGAAATTATTCTTCAAA+AGG + chr4_4:51822850-51822869 None:intergenic 25.0%
!!! TTGTTGGCATTTAATTTGTT+AGG - chr4_4:51823070-51823089 Msa0687090:CDS 25.0%
!! TAAATGGGTACTTAGATTTT+CGG - chr4_4:51823710-51823729 Msa0687090:five_prime_UTR 25.0%
!!! TTTTTTGGGTTTATGGATTT+TGG - chr4_4:51823734-51823753 Msa0687090:five_prime_UTR 25.0%
! AACCTTTAACGAATAAAAGA+CGG + chr4_4:51823838-51823857 None:intergenic 25.0%
AAATGAAATGAGGGAACTAT+GGG + chr4_4:51821560-51821579 None:intergenic 30.0%
TGAATATGATGATTGTGTTG+TGG + chr4_4:51821729-51821748 None:intergenic 30.0%
!!! TGGTTTTTTTGAGTGTTGAA+TGG + chr4_4:51821772-51821791 None:intergenic 30.0%
AGTAGTTTACATTACACACA+TGG - chr4_4:51821853-51821872 Msa0687090:CDS 30.0%
AAAGAGAGTGATATAAGTTG+AGG + chr4_4:51821888-51821907 None:intergenic 30.0%
!! AAGGTTGTGTTTTTACCTTT+GGG + chr4_4:51821929-51821948 None:intergenic 30.0%
!! AAAGGTTGTGTTTTTACCTT+TGG + chr4_4:51821930-51821949 None:intergenic 30.0%
!!! GTTAGCGTTTTTTGTTTTGA+AGG + chr4_4:51821975-51821994 None:intergenic 30.0%
! TAATTTTACCTTGGAGAAGA+TGG + chr4_4:51822686-51822705 None:intergenic 30.0%
ATTTCCACTTTCAGATTTGT+AGG - chr4_4:51822972-51822991 Msa0687090:CDS 30.0%
TTTCCACTTTCAGATTTGTA+GGG - chr4_4:51822973-51822992 Msa0687090:CDS 30.0%
TTAAAAACTAAGTTCCCCTT+TGG + chr4_4:51823024-51823043 None:intergenic 30.0%
!! AAGGGGAACTTAGTTTTTAA+TGG - chr4_4:51823024-51823043 Msa0687090:CDS 30.0%
AACTAGTCATCTTAAGCAAA+CGG - chr4_4:51823113-51823132 Msa0687090:CDS 30.0%
ATCTTAAGCAAACGGTTAAA+TGG - chr4_4:51823121-51823140 Msa0687090:CDS 30.0%
GATTGTAACAACTTGTGTTA+TGG - chr4_4:51823301-51823320 Msa0687090:CDS 30.0%
TGAAAATGGAGTTCAATACA+CGG - chr4_4:51823428-51823447 Msa0687090:CDS 30.0%
! TCTGCATTTGTTTACTCTTT+TGG - chr4_4:51823597-51823616 Msa0687090:CDS 30.0%
!!! TTTAGTTTTTAGCTAGCTGT+AGG - chr4_4:51823643-51823662 Msa0687090:five_prime_UTR 30.0%
! AAATGGGTACTTAGATTTTC+GGG - chr4_4:51823711-51823730 Msa0687090:five_prime_UTR 30.0%
!!! ACTTAGATTTTCGGGTTTTT+TGG - chr4_4:51823719-51823738 Msa0687090:five_prime_UTR 30.0%
!!! CTTAGATTTTCGGGTTTTTT+GGG - chr4_4:51823720-51823739 Msa0687090:five_prime_UTR 30.0%
! AATTTTCCCAACGAATCTTA+AGG + chr4_4:51823812-51823831 None:intergenic 30.0%
! TTCCGTCTTTTATTCGTTAA+AGG - chr4_4:51823833-51823852 Msa0687090:five_prime_UTR 30.0%
! TTTGAGGTTCTATGATTGTT+AGG - chr4_4:51823913-51823932 Msa0687090:five_prime_UTR 30.0%
TTGAGGTTCTATGATTGTTA+GGG - chr4_4:51823914-51823933 Msa0687090:five_prime_UTR 30.0%
CCAACATATTTCTCCAATTT+CGG - chr4_4:51823949-51823968 Msa0687090:five_prime_UTR 30.0%
CAACATATTTCTCCAATTTC+GGG - chr4_4:51823950-51823969 Msa0687090:five_prime_UTR 30.0%
AATGAAATGAGGGAACTATG+GGG + chr4_4:51821559-51821578 None:intergenic 35.0%
GAAATGAAATGAGGGAACTA+TGG + chr4_4:51821561-51821580 None:intergenic 35.0%
!!! GACAAAAGAGAGTGTTTTGA+GGG + chr4_4:51821607-51821626 None:intergenic 35.0%
!! TGTTGGGATTTGATAATGAG+TGG + chr4_4:51821645-51821664 None:intergenic 35.0%
ATGATGATTGTGTTGTGGAA+TGG + chr4_4:51821724-51821743 None:intergenic 35.0%
AAACCAAGCAACAACTAACT+AGG - chr4_4:51821786-51821805 Msa0687090:three_prime_UTR 35.0%
TAACCTAGTTAGTTGTTGCT+TGG + chr4_4:51821792-51821811 None:intergenic 35.0%
!! TGTTTTTGTTCAGGTATCAC+TGG - chr4_4:51822014-51822033 Msa0687090:CDS 35.0%
TCAAACATGGTAGAATGCAA+CGG - chr4_4:51822257-51822276 Msa0687090:intron 35.0%
AATCTACCCATGATTGTTGA+AGG - chr4_4:51822371-51822390 Msa0687090:intron 35.0%
AACATTTCCGAGTAAATCGA+CGG + chr4_4:51822569-51822588 None:intergenic 35.0%
! TACTTTTACTTGTACTGCTG+CGG - chr4_4:51822640-51822659 Msa0687090:intron 35.0%
AGAAGATGGACAAAAGGTAA+CGG + chr4_4:51822672-51822691 None:intergenic 35.0%
GTCATGTAATGTCATCATCA+TGG - chr4_4:51822921-51822940 Msa0687090:CDS 35.0%
AAACCCTACAAATCTGAAAG+TGG + chr4_4:51822979-51822998 None:intergenic 35.0%
GTTTGTTTCCATGTAACCAT+GGG - chr4_4:51822995-51823014 Msa0687090:CDS 35.0%
ATAAAATCCCACTTGCTCAA+AGG - chr4_4:51823214-51823233 Msa0687090:CDS 35.0%
TAAAATCCCACTTGCTCAAA+GGG - chr4_4:51823215-51823234 Msa0687090:CDS 35.0%
GAAAATGGAGTTCAATACAC+GGG - chr4_4:51823429-51823448 Msa0687090:CDS 35.0%
TATTCAGTTCCCGTAAATTC+AGG - chr4_4:51823471-51823490 Msa0687090:intron 35.0%
ATTCAGTTCCCGTAAATTCA+GGG - chr4_4:51823472-51823491 Msa0687090:intron 35.0%
TGGATCATATTTAGCTGGAT+TGG - chr4_4:51823545-51823564 Msa0687090:intron 35.0%
!!! TTTCGGGTTTTTTGGGTTTA+TGG - chr4_4:51823727-51823746 Msa0687090:five_prime_UTR 35.0%
AGAACCTCAAAAAGCTTCAT+GGG + chr4_4:51823904-51823923 None:intergenic 35.0%
TAGAACCTCAAAAAGCTTCA+TGG + chr4_4:51823905-51823924 None:intergenic 35.0%
TGAGGTTCTATGATTGTTAG+GGG - chr4_4:51823915-51823934 Msa0687090:five_prime_UTR 35.0%
CCGAAATTGGAGAAATATGT+TGG + chr4_4:51823952-51823971 None:intergenic 35.0%
ATTTATTAATCCGTCGACCC+AGG - chr4_4:51821516-51821535 Msa0687090:three_prime_UTR 40.0%
!!! GGACAAAAGAGAGTGTTTTG+AGG + chr4_4:51821608-51821627 None:intergenic 40.0%
TGAGTGGAGTTAGCAAGAAA+AGG + chr4_4:51821629-51821648 None:intergenic 40.0%
!! TTAGGAAGGAGAGTGTTGTT+GGG + chr4_4:51821661-51821680 None:intergenic 40.0%
!! TTTAGGAAGGAGAGTGTTGT+TGG + chr4_4:51821662-51821681 None:intergenic 40.0%
TGTTACAAGTGAAGTGAGTG+AGG + chr4_4:51821687-51821706 None:intergenic 40.0%
ATGATTGTGTTGTGGAATGG+TGG + chr4_4:51821721-51821740 None:intergenic 40.0%
!! TGTTTTTACCTTTGGGAGTG+AGG + chr4_4:51821922-51821941 None:intergenic 40.0%
ACAAATGTGCCTACACAGTA+TGG - chr4_4:51822057-51822076 Msa0687090:CDS 40.0%
CCAGAACCTTCAACAATCAT+GGG + chr4_4:51822380-51822399 None:intergenic 40.0%
ACCAGAACCTTCAACAATCA+TGG + chr4_4:51822381-51822400 None:intergenic 40.0%
! ATGATTGTTGAAGGTTCTGG+TGG - chr4_4:51822380-51822399 Msa0687090:intron 40.0%
! TGATTGTTGAAGGTTCTGGT+GGG - chr4_4:51822381-51822400 Msa0687090:intron 40.0%
! AGTCCTGAGTATTGTTGTAG+TGG - chr4_4:51822515-51822534 Msa0687090:intron 40.0%
AAGCGTAGCTATACGATCTA+GGG + chr4_4:51822604-51822623 None:intergenic 40.0%
TAAGCGTAGCTATACGATCT+AGG + chr4_4:51822605-51822624 None:intergenic 40.0%
! GGTTTGTTTCCATGTAACCA+TGG - chr4_4:51822994-51823013 Msa0687090:CDS 40.0%
GTTGGAACAGGATCTGAAAA+TGG - chr4_4:51823414-51823433 Msa0687090:CDS 40.0%
TCCTGAATTAGATGCTGATC+CGG + chr4_4:51823499-51823518 None:intergenic 40.0%
TCAGCATCTAATTCAGGATC+AGG - chr4_4:51823501-51823520 Msa0687090:intron 40.0%
ATATGATCCATCTGCAAGCA+TGG + chr4_4:51823535-51823554 None:intergenic 40.0%
GCAGATGGATCATATTTAGC+TGG - chr4_4:51823540-51823559 Msa0687090:intron 40.0%
!! ATATTTAGCTGGATTGGCCA+TGG - chr4_4:51823551-51823570 Msa0687090:intron 40.0%
!! TATTTAGCTGGATTGGCCAT+GGG - chr4_4:51823552-51823571 Msa0687090:intron 40.0%
TGCAGATGAAGTTGTTGTAG+AGG + chr4_4:51823583-51823602 None:intergenic 40.0%
! GCACTGTTTTCGTGGTATTT+TGG - chr4_4:51823671-51823690 Msa0687090:five_prime_UTR 40.0%
AAGGTGGGTGGTTTACAATA+AGG + chr4_4:51823793-51823812 None:intergenic 40.0%
TTCCCAACGAATCTTAAGGT+GGG + chr4_4:51823808-51823827 None:intergenic 40.0%
TTTCCCAACGAATCTTAAGG+TGG + chr4_4:51823809-51823828 None:intergenic 40.0%
GAACCTCAAAAAGCTTCATG+GGG + chr4_4:51823903-51823922 None:intergenic 40.0%
GAGGTTCTATGATTGTTAGG+GGG - chr4_4:51823916-51823935 Msa0687090:five_prime_UTR 40.0%
CAACAAAGATAGCCCGAAAT+TGG + chr4_4:51823965-51823984 None:intergenic 40.0%
CAATTTCGGGCTATCTTTGT+TGG - chr4_4:51823963-51823982 Msa0687090:five_prime_UTR 40.0%
!!! GTTTTGAGGGTAAAAGCTGC+TGG + chr4_4:51821594-51821613 None:intergenic 45.0%
AGTGAGTGAGGAGTTTAGGA+AGG + chr4_4:51821675-51821694 None:intergenic 45.0%
GTGAAGTGAGTGAGGAGTTT+AGG + chr4_4:51821679-51821698 None:intergenic 45.0%
ATTGTGTTGTGGAATGGTGG+TGG + chr4_4:51821718-51821737 None:intergenic 45.0%
! CTTCTTTTCCTCACTCCCAA+AGG - chr4_4:51821911-51821930 Msa0687090:CDS 45.0%
AATACCTGGCCATACTGTGT+AGG + chr4_4:51822069-51822088 None:intergenic 45.0%
TTCTTGTGGAAGCTCGAAAC+CGG + chr4_4:51822126-51822145 None:intergenic 45.0%
AAAGTGCGAGTGGTTTCTTG+TGG + chr4_4:51822140-51822159 None:intergenic 45.0%
GAGTTCACTCTTGGCACTAT+AGG - chr4_4:51822311-51822330 Msa0687090:intron 45.0%
CCCATGATTGTTGAAGGTTC+TGG - chr4_4:51822377-51822396 Msa0687090:intron 45.0%
TATTGTTGTAGTGGCGCGTA+TGG - chr4_4:51822524-51822543 Msa0687090:intron 45.0%
!! TTGTAGGCCGTCGATTTACT+CGG - chr4_4:51822559-51822578 Msa0687090:intron 45.0%
CCTTGGAGAAGATGGACAAA+AGG + chr4_4:51822678-51822697 None:intergenic 45.0%
! CCTTTTGTCCATCTTCTCCA+AGG - chr4_4:51822675-51822694 Msa0687090:intron 45.0%
TTTGGCTACCCATGGTTACA+TGG + chr4_4:51823006-51823025 None:intergenic 45.0%
ATGTAACCATGGGTAGCCAA+AGG - chr4_4:51823005-51823024 Msa0687090:CDS 45.0%
TGTAACCATGGGTAGCCAAA+GGG - chr4_4:51823006-51823025 Msa0687090:CDS 45.0%
ACACTACCCTTTGAGCAAGT+GGG + chr4_4:51823224-51823243 None:intergenic 45.0%
TACACTACCCTTTGAGCAAG+TGG + chr4_4:51823225-51823244 None:intergenic 45.0%
GTTGAGGTCATTGGAGTTGT+TGG + chr4_4:51823354-51823373 None:intergenic 45.0%
!! GGTATTGGTGTTGAGGTCAT+TGG + chr4_4:51823363-51823382 None:intergenic 45.0%
ATGACCTCAACACCAATACC+AGG - chr4_4:51823363-51823382 Msa0687090:CDS 45.0%
ACGGGAACTGAATACCCAAT+GGG + chr4_4:51823465-51823484 None:intergenic 45.0%
TACGGGAACTGAATACCCAA+TGG + chr4_4:51823466-51823485 None:intergenic 45.0%
TGATCCGGACCCTGAATTTA+CGG + chr4_4:51823484-51823503 None:intergenic 45.0%
TCCGGATCAGCATCTAATTC+AGG - chr4_4:51823495-51823514 Msa0687090:intron 45.0%
GCATCTAATTCAGGATCAGG+AGG - chr4_4:51823504-51823523 Msa0687090:intron 45.0%
CACCCACCTTAAGATTCGTT+GGG - chr4_4:51823803-51823822 Msa0687090:five_prime_UTR 45.0%
! TCTCCCCATGAAGCTTTTTG+AGG - chr4_4:51823897-51823916 Msa0687090:five_prime_UTR 45.0%
CTATTGGATGCCCATGACCT+GGG + chr4_4:51821536-51821555 None:intergenic 50.0%
TGAGGGAACTATGGGGCTAT+TGG + chr4_4:51821552-51821571 None:intergenic 50.0%
CCAGCATTTGCGAGAATACC+TGG + chr4_4:51822083-51822102 None:intergenic 50.0%
CCAGGTATTCTCGCAAATGC+TGG - chr4_4:51822080-51822099 Msa0687090:CDS 50.0%
AATGCTGGTAGCCCAACACT+CGG - chr4_4:51822095-51822114 Msa0687090:CDS 50.0%
CATGTTTGAGCCACAGTCTC+CGG + chr4_4:51822246-51822265 None:intergenic 50.0%
ATGGTAGAATGCAACGGTGC+CGG - chr4_4:51822263-51822282 Msa0687090:intron 50.0%
ACCCTAGCTGAGTTCACTCT+TGG - chr4_4:51822302-51822321 Msa0687090:intron 50.0%
GCCAAGAGTGAACTCAGCTA+GGG + chr4_4:51822306-51822325 None:intergenic 50.0%
TGCCAAGAGTGAACTCAGCT+AGG + chr4_4:51822307-51822326 None:intergenic 50.0%
TACGACGTGAGTCTCGTTGA+TGG - chr4_4:51822344-51822363 Msa0687090:intron 50.0%
! TGTTGAAGGTTCTGGTGGGT+CGG - chr4_4:51822385-51822404 Msa0687090:intron 50.0%
GGATCTGAACCAGCAATGTC+CGG - chr4_4:51822436-51822455 Msa0687090:intron 50.0%
GCGCCACTACAACAATACTC+AGG + chr4_4:51822521-51822540 None:intergenic 50.0%
! ATGGTTCACCGGCTACTTGT+AGG - chr4_4:51822543-51822562 Msa0687090:intron 50.0%
GTAACCATGGGTAGCCAAAG+GGG - chr4_4:51823007-51823026 Msa0687090:CDS 50.0%
!! TGCTCCTGGTATTGGTGTTG+AGG + chr4_4:51823370-51823389 None:intergenic 50.0%
TCAACACCAATACCAGGAGC+AGG - chr4_4:51823369-51823388 Msa0687090:CDS 50.0%
CAACACCAATACCAGGAGCA+GGG - chr4_4:51823370-51823389 Msa0687090:CDS 50.0%
TCAGATCCTGTTCCAACCTC+AGG + chr4_4:51823411-51823430 None:intergenic 50.0%
GTTCCCGTAAATTCAGGGTC+CGG - chr4_4:51823477-51823496 Msa0687090:intron 50.0%
GATCCGGACCCTGAATTTAC+GGG + chr4_4:51823483-51823502 None:intergenic 50.0%
GGAGAAACCATGCTTGCAGA+TGG - chr4_4:51823525-51823544 Msa0687090:intron 50.0%
! AGGCTGTAGCACTGTTTTCG+TGG - chr4_4:51823663-51823682 Msa0687090:five_prime_UTR 50.0%
!! GGCTTGTTGCTCACTAAAGC+TGG - chr4_4:51823755-51823774 Msa0687090:five_prime_UTR 50.0%
CCAACGAATCTTAAGGTGGG+TGG + chr4_4:51823805-51823824 None:intergenic 50.0%
CCACCCACCTTAAGATTCGT+TGG - chr4_4:51823802-51823821 Msa0687090:five_prime_UTR 50.0%
TAATCCGTCGACCCAGGTCA+TGG - chr4_4:51821522-51821541 Msa0687090:three_prime_UTR 55.0%
AATCCGTCGACCCAGGTCAT+GGG - chr4_4:51821523-51821542 Msa0687090:three_prime_UTR 55.0%
GCTATTGGATGCCCATGACC+TGG + chr4_4:51821537-51821556 None:intergenic 55.0%
TGTGCCTACACAGTATGGCC+AGG - chr4_4:51822062-51822081 Msa0687090:CDS 55.0%
AACCGGTGCTTCCGAGTGTT+GGG + chr4_4:51822109-51822128 None:intergenic 55.0%
AAACCGGTGCTTCCGAGTGT+TGG + chr4_4:51822110-51822129 None:intergenic 55.0%
! TGGAGCTTGGAAAGTGCGAG+TGG + chr4_4:51822150-51822169 None:intergenic 55.0%
CGAACCGGTTGCAACTTCGA+CGG - chr4_4:51822197-51822216 Msa0687090:intron 55.0%
GGATCCGTCGAAGTTGCAAC+CGG + chr4_4:51822204-51822223 None:intergenic 55.0%
GGTTGCAACTTCGACGGATC+CGG - chr4_4:51822203-51822222 Msa0687090:intron 55.0%
GTCTCCGGTGAGACATGAAC+CGG + chr4_4:51822231-51822250 None:intergenic 55.0%
TCATGTCTCACCGGAGACTG+TGG - chr4_4:51822233-51822252 Msa0687090:intron 55.0%
CGGAGACTGTGGCTCAAACA+TGG - chr4_4:51822244-51822263 Msa0687090:intron 55.0%
AACTCAGCTAGGGTTGCTGG+TGG + chr4_4:51822296-51822315 None:intergenic 55.0%
GTGAACTCAGCTAGGGTTGC+TGG + chr4_4:51822299-51822318 None:intergenic 55.0%
! GTTGAAGGTTCTGGTGGGTC+GGG - chr4_4:51822386-51822405 Msa0687090:intron 55.0%
TCGGGTATGTGTGAGTCCAC+CGG - chr4_4:51822404-51822423 Msa0687090:intron 55.0%
TCTGAACCAGCAATGTCCGG+CGG - chr4_4:51822439-51822458 Msa0687090:intron 55.0%
! TCTGAAGCTCTCAACTCCGC+CGG + chr4_4:51822458-51822477 None:intergenic 55.0%
GCGGAGTTGAGAGCTTCAGA+TGG - chr4_4:51822458-51822477 Msa0687090:intron 55.0%
!! AAGAGCGCGTGTGAGGCTTT+TGG - chr4_4:51822491-51822510 Msa0687090:intron 55.0%
TAGTGGCGCGTATGGTTCAC+CGG - chr4_4:51822532-51822551 Msa0687090:intron 55.0%
ATCGACGGCCTACAAGTAGC+CGG + chr4_4:51822554-51822573 None:intergenic 55.0%
AGTTCCCCTTTGGCTACCCA+TGG + chr4_4:51823014-51823033 None:intergenic 55.0%
ACTGTCCCTGCTCCTGGTAT+TGG + chr4_4:51823378-51823397 None:intergenic 55.0%
CAGGAGCAGGGACAGTGATA+GGG - chr4_4:51823382-51823401 Msa0687090:CDS 55.0%
GACAGTGATAGGGACTCCTG+AGG - chr4_4:51823392-51823411 Msa0687090:CDS 55.0%
GTGATAGGGACTCCTGAGGT+TGG - chr4_4:51823396-51823415 Msa0687090:CDS 55.0%
ACGGGTACAGACACACCCAT+TGG - chr4_4:51823447-51823466 Msa0687090:intron 55.0%
CGGGTACAGACACACCCATT+GGG - chr4_4:51823448-51823467 Msa0687090:intron 55.0%
!! TTAGCTGGATTGGCCATGGG+AGG - chr4_4:51823555-51823574 Msa0687090:intron 55.0%
TGTTGTAGAGGAGCCTCCCA+TGG + chr4_4:51823571-51823590 None:intergenic 55.0%
ATGCCCATGACCTGGGTCGA+CGG + chr4_4:51821529-51821548 None:intergenic 60.0%
! AGCCCAACACTCGGAAGCAC+CGG - chr4_4:51822104-51822123 Msa0687090:CDS 60.0%
CGCACTTTCCAAGCTCCAGC+CGG - chr4_4:51822152-51822171 Msa0687090:intron 60.0%
CTTTCCAAGCTCCAGCCGGT+TGG - chr4_4:51822156-51822175 Msa0687090:intron 60.0%
!! AACCGGTTCGAGCCCAGAAC+CGG + chr4_4:51822187-51822206 None:intergenic 60.0%
AACTTCGACGGATCCGGCAC+CGG - chr4_4:51822209-51822228 Msa0687090:intron 60.0%
GGTGAGACATGAACCGGTGC+CGG + chr4_4:51822225-51822244 None:intergenic 60.0%
!! GGCACCGGTTCATGTCTCAC+CGG - chr4_4:51822224-51822243 Msa0687090:intron 60.0%
CGGGTATGTGTGAGTCCACC+GGG - chr4_4:51822405-51822424 Msa0687090:intron 60.0%
GTTCAGATCCGACGTGCACC+CGG + chr4_4:51822426-51822445 None:intergenic 60.0%
TCAACTCCGCCGGACATTGC+TGG + chr4_4:51822448-51822467 None:intergenic 60.0%
CCTATCACTGTCCCTGCTCC+TGG + chr4_4:51823384-51823403 None:intergenic 60.0%
CCAGGAGCAGGGACAGTGAT+AGG - chr4_4:51823381-51823400 Msa0687090:CDS 60.0%
GGGACTCCTGAGGTTGGAAC+AGG - chr4_4:51823402-51823421 Msa0687090:CDS 60.0%
! CAAGCTCCAGCCGGTTGGTC+CGG - chr4_4:51822161-51822180 Msa0687090:intron 65.0%
! GGTTGCTGGTGGTGCAGCTC+CGG + chr4_4:51822285-51822304 None:intergenic 65.0%
TGAGTCCACCGGGTGCACGT+CGG - chr4_4:51822415-51822434 Msa0687090:intron 65.0%
! CGCGTGTAAGAGCGCGTGTG+AGG - chr4_4:51822484-51822503 Msa0687090:intron 65.0%
CGGACCAACCGGCTGGAGCT+TGG + chr4_4:51822163-51822182 None:intergenic 70.0%
CCAGAACCGGCCGGACCAAC+CGG + chr4_4:51822174-51822193 None:intergenic 70.0%
! CGGTTGGTCCGGCCGGTTCT+GGG - chr4_4:51822172-51822191 Msa0687090:intron 70.0%
GGTTCGAGCCCAGAACCGGC+CGG + chr4_4:51822183-51822202 None:intergenic 70.0%
!! GGCCGGTTCTGGGCTCGAAC+CGG - chr4_4:51822182-51822201 Msa0687090:intron 70.0%
CAGATCCGACGTGCACCCGG+TGG + chr4_4:51822423-51822442 None:intergenic 70.0%
! CTCCAGCCGGTTGGTCCGGC+CGG - chr4_4:51822165-51822184 Msa0687090:intron 75.0%
AACCGGCCGGACCAACCGGC+TGG + chr4_4:51822170-51822189 None:intergenic 75.0%
! CCGGTTGGTCCGGCCGGTTC+TGG - chr4_4:51822171-51822190 Msa0687090:intron 75.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 51821480 51824011 51821480 ID=Msa0687090;Name=Msa0687090
chr4_4 mRNA 51821480 51824011 51821480 ID=Msa0687090-mRNA-1;Parent=Msa0687090;Name=Msa0687090-mRNA-1;_AED=0.02;_eAED=0.02;_QI=392|1|1|1|1|1|3|364|348
chr4_4 exon 51821480 51822159 51821480 ID=Msa0687090-mRNA-1:exon:13480;Parent=Msa0687090-mRNA-1
chr4_4 exon 51822795 51823464 51822795 ID=Msa0687090-mRNA-1:exon:13479;Parent=Msa0687090-mRNA-1
chr4_4 exon 51823559 51824011 51823559 ID=Msa0687090-mRNA-1:exon:13478;Parent=Msa0687090-mRNA-1
chr4_4 five_prime_UTR 51823620 51824011 51823620 ID=Msa0687090-mRNA-1:five_prime_utr;Parent=Msa0687090-mRNA-1
chr4_4 CDS 51823559 51823619 51823559 ID=Msa0687090-mRNA-1:cds;Parent=Msa0687090-mRNA-1
chr4_4 CDS 51822795 51823464 51822795 ID=Msa0687090-mRNA-1:cds;Parent=Msa0687090-mRNA-1
chr4_4 CDS 51821844 51822159 51821844 ID=Msa0687090-mRNA-1:cds;Parent=Msa0687090-mRNA-1
chr4_4 three_prime_UTR 51821480 51821843 51821480 ID=Msa0687090-mRNA-1:three_prime_utr;Parent=Msa0687090-mRNA-1
Gene Sequence

>Msa0687090

ATGGATCTACTAGCCTCAACTTATATCACTCTCTTTACTCTTCTTTTCCTCACTCCCAAAGGTATCACTGGTTCAACTTTCACATTTGTGAACAAATGTGCCTACACAGTATGGCCAGGTATTCTCGCAAATGCTGGTAGCCCAACACTCGGAAGCACCGGTTTCGAGCTTCCACAAGAAACCACTCGCACTTTCCAAGCTCCAGCCGGTTGGTCCGGCCGGTTCTGGGCTCGAACCGGTTGCAACTTCGACGGATCCGGCACCGGTTCATGTCTCACCGGAGACTGTGGCTCAAACATGGTAGAATGCAACGGTGCCGGAGCTGCACCACCAGCAACCCTAGCTGAGTTCACTCTTGGCACTATAGGACAAGATTTCTACGACGTGAGTCTCGTTGATGGCTACAATCTACCCATGATTGTTGAAGGTTCTGGTGGGTCGGGTATGTGTGAGTCCACCGGGTGCACGTCGGATCTGAACCAGCAATGTCCGGCGGAGTTGAGAGCTTCAGATGGAAGCGCGTGTAAGAGCGCGTGTGAGGCTTTTGGTAGTCCTGAGTATTGTTGTAGTGGCGCGTATGGTTCACCGGCTACTTGTAGGCCGTCGATTTACTCGGAAATGTTTAAAGCTGCTTGCCCTAGATCGTATAGCTACGCTTACGATGATGCTTCGAGTACTTTTACTTGTACTGCTGCGGATTATACCGTTACCTTTTGTCCATCTTCTCCAAGCAAAAAATCATCACAATATCCAACAACTCCAATGACCTCAACACCAATACCAGGAGCAGGGACAGTGATAGGGACTCCTGAGGTTGGAACAGGATCTGAAAATGGAGTTCAATACACGGGTACAGACACACCCATTGGGTATTCAGTTCCCGTAAATTCAGGGTCCGGATCAGCATCTAATTCAGGATCAGGAGGAGAAACCATGCTTGCAGATGGATCATATTTAGCTGGATTGGCCATGGGAGGCTCCTCTACAACAACTTCATCTGCATTTGTTTACTCTTTTGGTTTTTTTCTTATTTTCTTCTTGCTTTAG
Protein sequence

>Msa0687090

MDLLASTYITLFTLLFLTPKGITGSTFTFVNKCAYTVWPGILANAGSPTLGSTGFELPQETTRTFQAPAGWSGRFWARTGCNFDGSGTGSCLTGDCGSNMVECNGAGAAPPATLAEFTLGTIGQDFYDVSLVDGYNLPMIVEGSGGSGMCESTGCTSDLNQQCPAELRASDGSACKSACEAFGSPEYCCSGAYGSPATCRPSIYSEMFKAACPRSYSYAYDDASSTFTCTAADYTVTFCPSSPSKKSSQYPTTPMTSTPIPGAGTVIGTPEVGTGSENGVQYTGTDTPIGYSVPVNSGSGSASNSGSGGETMLADGSYLAGLAMGGSSTTTSSAFVYSFGFFLIFFLL*