AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1158440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1158440 MtrunA17_Chr7g0253101 94.834 271 4 3 1 271 1 261 0.0 509
Msa1158440 MtrunA17_Chr6g0461591 62.409 274 73 10 1 261 1 257 4.23e-104 303
Msa1158440 MtrunA17_Chr3g0140331 39.200 250 95 9 15 260 121 317 5.02e-35 129
Msa1158440 MtrunA17_Chr1g0199671 76.471 68 16 0 193 260 239 306 5.26e-34 125
Msa1158440 MtrunA17_Chr4g0035891 71.795 78 19 1 196 270 211 288 1.80e-33 123
Msa1158440 MtrunA17_Chr2g0326771 57.944 107 39 2 172 272 191 297 1.82e-33 124
Msa1158440 MtrunA17_Chr5g0404981 41.176 238 100 9 45 269 163 373 2.74e-33 125
Msa1158440 MtrunA17_Chr8g0372291 77.465 71 16 0 196 266 218 288 3.60e-33 122
Msa1158440 MtrunA17_Chr1g0146231 35.985 264 114 9 11 260 76 298 7.27e-33 123
Msa1158440 MtrunA17_Chr8g0340401 37.118 229 78 7 45 260 115 290 3.07e-32 121
Msa1158440 MtrunA17_Chr1g0213161 75.758 66 16 0 196 261 152 217 6.53e-32 117
Msa1158440 MtrunA17_Chr1g0169431 74.648 71 18 0 190 260 166 236 6.59e-32 118
Msa1158440 MtrunA17_Chr4g0028961 72.222 72 20 0 193 264 229 300 9.60e-32 119
Msa1158440 MtrunA17_Chr3g0145121 70.833 72 21 0 196 267 215 286 1.60e-31 118
Msa1158440 MtrunA17_Chr5g0395391 73.846 65 17 0 196 260 270 334 3.13e-29 113
Msa1158440 MtrunA17_Chr5g0395391 71.875 64 18 0 196 259 168 231 1.44e-27 108
Msa1158440 MtrunA17_Chr7g0232671 58.065 62 26 0 196 257 187 248 6.00e-20 86.7
Msa1158440 MtrunA17_Chr7g0232701 56.923 65 28 0 193 257 70 134 7.63e-20 83.2
Msa1158440 MtrunA17_Chr7g0232681 40.000 105 63 0 153 257 89 193 2.92e-19 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1158440 AT4G32890.1 42.793 222 81 6 45 260 80 261 9.64e-44 150
Msa1158440 AT2G45050.1 38.618 246 98 8 21 266 57 249 4.07e-40 140
Msa1158440 AT5G66320.2 40.816 245 112 8 28 260 90 313 3.87e-36 132
Msa1158440 AT5G66320.1 40.816 245 112 8 28 260 90 313 3.87e-36 132
Msa1158440 AT3G60530.1 40.553 217 57 9 45 260 77 222 9.13e-36 128
Msa1158440 AT5G25830.1 38.235 238 89 8 45 260 82 283 2.60e-35 129
Msa1158440 AT3G24050.1 75.000 68 17 0 193 260 191 258 4.30e-33 122
Msa1158440 AT4G36240.1 38.532 218 71 6 45 261 74 229 7.35e-33 120
Msa1158440 AT3G54810.1 77.612 67 15 0 194 260 227 293 1.78e-32 122
Msa1158440 AT3G54810.2 77.612 67 15 0 194 260 227 293 1.78e-32 122
Msa1158440 AT3G51080.1 81.538 65 12 0 196 260 221 285 5.02e-32 120
Msa1158440 AT1G08010.2 72.308 65 18 0 196 260 220 284 3.65e-31 117
Msa1158440 AT1G08010.1 72.308 65 18 0 196 260 220 284 3.65e-31 117
Msa1158440 AT1G08010.3 72.308 65 18 0 196 260 220 284 3.65e-31 117
Msa1158440 AT1G08000.2 73.846 65 17 0 196 260 218 282 7.54e-30 114
Msa1158440 AT1G08000.1 73.846 65 17 0 196 260 218 282 7.54e-30 114
Msa1158440 AT4G34680.1 72.308 65 18 0 196 260 180 244 9.11e-30 113
Msa1158440 AT4G34680.2 72.308 65 18 0 196 260 180 244 9.11e-30 113
Msa1158440 AT2G28340.1 65.672 67 23 0 198 264 193 259 1.44e-26 105
Msa1158440 AT3G45170.1 56.579 76 33 0 185 260 104 179 5.25e-26 102

Find 69 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAGGTGAAATTGACTATT+TGG 0.123062 7_4:-47839464 Msa1158440:three_prime_UTR
CTCTCTTAAAACAAGCTTAT+TGG 0.124697 7_4:-47839925 Msa1158440:CDS
CACTAGAAACTTGTACATTT+TGG 0.144962 7_4:+47840649 None:intergenic
AACTTAGAGAGCACCAATTT+TGG 0.237029 7_4:-47840772 Msa1158440:five_prime_UTR
CATGCATTGCATAGTGTCTT+TGG 0.243410 7_4:+47839692 None:intergenic
TGAAAAGGAAATTGTGAAAA+AGG 0.260278 7_4:-47839837 Msa1158440:CDS
CATAGTGTCTTTGGGCCTAA+TGG 0.278012 7_4:+47839701 None:intergenic
ACACTACTCACTAGGATTAA+TGG 0.294145 7_4:+47839535 None:intergenic
GGAAACATGAGATAGTATTT+AGG 0.308712 7_4:-47839443 Msa1158440:three_prime_UTR
ATGGGCCTGCCCTCCATTGT+GGG 0.318681 7_4:+47839720 None:intergenic
CAATGGAGGGCAGGCCCATT+AGG 0.322478 7_4:-47839716 Msa1158440:CDS
AAGATGCTTAAAGGGTCTTT+TGG 0.328925 7_4:+47839995 None:intergenic
GTTGTTAGTGCTATGATTAT+TGG 0.333550 7_4:-47839417 Msa1158440:three_prime_UTR
GTATTGGATAAGATGCTTAA+AGG 0.338824 7_4:+47839986 None:intergenic
ATAGTGTCTTTGGGCCTAAT+GGG 0.339035 7_4:+47839702 None:intergenic
CACACCAAGAGAGGGGAAAA+TGG 0.339492 7_4:+47840727 None:intergenic
GGTTTCTTCACTGTGTTTGA+AGG 0.343748 7_4:+47840100 None:intergenic
AGAGAAAAGAAAGCCAAAAT+TGG 0.351490 7_4:+47840759 None:intergenic
GTACACCTCCTTGTTGGAAT+TGG 0.356244 7_4:+47839764 None:intergenic
TATGAAAGTGAAATGCAATA+TGG 0.367111 7_4:+47839324 None:intergenic
ATGCATTGCATAGTGTCTTT+GGG 0.368727 7_4:+47839693 None:intergenic
AATGGGCCTGCCCTCCATTG+TGG 0.406749 7_4:+47839719 None:intergenic
AGAAAGAGACAAAATGAAAC+AGG 0.417263 7_4:+47839364 None:intergenic
TATTGGATAAGATGCTTAAA+GGG 0.429212 7_4:+47839987 None:intergenic
CAATGTGTACACCTCCTTGT+TGG 0.437336 7_4:+47839758 None:intergenic
AGATTATGAAGTGGTTGAAA+AGG 0.475929 7_4:-47839852 Msa1158440:CDS
GTAAAAGTACACAACTTTGT+GGG 0.484296 7_4:+47840164 None:intergenic
TAGTGAACTCATGGTACCTA+AGG 0.486078 7_4:-47839894 Msa1158440:CDS
GGTAAAAGTACACAACTTTG+TGG 0.499157 7_4:+47840163 None:intergenic
CAGGCAAAGCAAGAAGCAAA+AGG 0.500793 7_4:-47840042 Msa1158440:CDS
TAAAAGGTGAACTGAGACAA+GGG 0.508516 7_4:-47840804 None:intergenic
AGGACCCCACAATGGAGGGC+AGG 0.512119 7_4:-47839725 Msa1158440:CDS
ATTATGTAGGAAGTGGATGT+TGG 0.517271 7_4:-47840229 Msa1158440:intron
ATCCTAGTGAGTAGTGTTGG+TGG 0.530927 7_4:-47839529 Msa1158440:exon
TTGAAAGGTTAGAGCAAGAA+GGG 0.531774 7_4:+47840601 None:intergenic
CTTGAAAGGTTAGAGCAAGA+AGG 0.532182 7_4:+47840600 None:intergenic
ACAAAAGTTGGACTCTTAGC+AGG 0.532481 7_4:+47839626 None:intergenic
TAGTGAGTGCAAACAAGAGA+AGG 0.541293 7_4:-47840631 Msa1158440:CDS
GTTAGCTGATAGTGAACTCA+TGG 0.566356 7_4:-47839903 Msa1158440:CDS
TGGGCCTGCCCTCCATTGTG+GGG 0.567754 7_4:+47839721 None:intergenic
GGTCATCAAGGCTACTTGAA+AGG 0.569542 7_4:+47840586 None:intergenic
GAGCAGAAAAGAGGTCATCA+AGG 0.570986 7_4:+47840574 None:intergenic
CAAGAGAGGGGAAAATGGTA+AGG 0.576200 7_4:+47840732 None:intergenic
TGTGGTGTGAGGTACAAATC+TGG 0.576464 7_4:-47839671 Msa1158440:CDS
CTCTTAGCAGGTCTATACTC+TGG 0.579993 7_4:+47839638 None:intergenic
GTAGGAAGTGGATGTTGGAA+TGG 0.583317 7_4:-47840224 Msa1158440:intron
TTAATCCTAGTGAGTAGTGT+TGG 0.586069 7_4:-47839532 Msa1158440:exon
TTTGAGGTTGATGTTAACAA+TGG 0.593552 7_4:-47839794 Msa1158440:CDS
ACAAAGGACCCCACAATGGA+GGG 0.595325 7_4:-47839729 Msa1158440:CDS
TAATTTGAACACACCAAGAG+AGG 0.599833 7_4:+47840718 None:intergenic
CACAAAGGACCCCACAATGG+AGG 0.605327 7_4:-47839730 Msa1158440:CDS
ACAAGAAAGTCATGGAGATG+AGG 0.609025 7_4:-47839580 Msa1158440:CDS
AAATTCACACAAGAAAGTCA+TGG 0.613481 7_4:-47839588 Msa1158440:CDS
AATTTGAACACACCAAGAGA+GGG 0.616813 7_4:+47840719 None:intergenic
GGTAACAAAAGATTATGAAG+TGG 0.619135 7_4:-47839861 Msa1158440:CDS
ATATGGTATGCAACATGAGG+AGG 0.623003 7_4:+47839341 None:intergenic
AGTGAACTCATGGTACCTAA+GGG 0.625585 7_4:-47839893 Msa1158440:CDS
AAGTGGATGTTGGAATGGAA+TGG 0.634243 7_4:-47840219 Msa1158440:CDS
GCAATATGGTATGCAACATG+AGG 0.637604 7_4:+47839338 None:intergenic
AACCACCAACACTACTCACT+AGG 0.638539 7_4:+47839527 None:intergenic
GTCAAAATCCAATTCCAACA+AGG 0.638845 7_4:-47839772 Msa1158440:CDS
ATAAAAGGTGAACTGAGACA+AGG 0.649757 7_4:-47840805 None:intergenic
GTACACATTGTTTGTCACAA+AGG 0.655851 7_4:-47839745 Msa1158440:CDS
GTGTGAGGTACAAATCTGGT+AGG 0.664094 7_4:-47839667 Msa1158440:CDS
ATTTGAACACACCAAGAGAG+GGG 0.671203 7_4:+47840720 None:intergenic
TGTCACAAAGGACCCCACAA+TGG 0.681006 7_4:-47839733 Msa1158440:CDS
AAAATCCAATTCCAACAAGG+AGG 0.704851 7_4:-47839769 Msa1158440:CDS
TATGCAATGCATGTGGTGTG+AGG 0.734829 7_4:-47839682 Msa1158440:CDS
AAGACACTATGCAATGCATG+TGG 0.751831 7_4:-47839689 Msa1158440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAGAAACATAATATTAA+AGG + chr7_4:47839779-47839798 None:intergenic 10.0%
!!! TAAATCAAAGTTTAAATTTT+AGG - chr7_4:47840630-47840649 Msa1158440:CDS 10.0%
!! AATTAGAAACATAATATTAA+AGG + chr7_4:47839779-47839798 None:intergenic 10.0%
!!! TAAATCAAAGTTTAAATTTT+AGG - chr7_4:47840630-47840649 Msa1158440:CDS 10.0%
!!! TATTATGTTTCTAATTTTCT+TGG - chr7_4:47839783-47839802 Msa1158440:CDS 15.0%
!!! TATTATGTTTCTAATTTTCT+TGG - chr7_4:47839783-47839802 Msa1158440:CDS 15.0%
!!! TGTAGTTTTTGCTAAAATTA+TGG - chr7_4:47839827-47839846 Msa1158440:CDS 20.0%
!!! TGTAGTTTTTGCTAAAATTA+TGG - chr7_4:47839827-47839846 Msa1158440:CDS 20.0%
! TAAGGTGTAGAAAAAGTTAA+GGG + chr7_4:47839426-47839445 None:intergenic 25.0%
! ATAAGGTGTAGAAAAAGTTA+AGG + chr7_4:47839427-47839446 None:intergenic 25.0%
!! TTTTCTACACCTTATCAAAA+TGG - chr7_4:47839432-47839451 Msa1158440:three_prime_UTR 25.0%
! GAAAAACATATGTTAGAACA+AGG + chr7_4:47839692-47839711 None:intergenic 25.0%
! TTTGACCAAACAAATTATGT+AGG - chr7_4:47839869-47839888 Msa1158440:CDS 25.0%
! TATTGGATAAGATGCTTAAA+GGG + chr7_4:47840127-47840146 None:intergenic 25.0%
! TGAAAAGGAAATTGTGAAAA+AGG - chr7_4:47840274-47840293 Msa1158440:intron 25.0%
! TTTAGGTGAAATTGACTATT+TGG - chr7_4:47840647-47840666 Msa1158440:CDS 25.0%
! TATGAAAGTGAAATGCAATA+TGG + chr7_4:47840790-47840809 None:intergenic 25.0%
! TAAGGTGTAGAAAAAGTTAA+GGG + chr7_4:47839426-47839445 None:intergenic 25.0%
! ATAAGGTGTAGAAAAAGTTA+AGG + chr7_4:47839427-47839446 None:intergenic 25.0%
!! TTTTCTACACCTTATCAAAA+TGG - chr7_4:47839432-47839451 Msa1158440:three_prime_UTR 25.0%
! GAAAAACATATGTTAGAACA+AGG + chr7_4:47839692-47839711 None:intergenic 25.0%
! TTTGACCAAACAAATTATGT+AGG - chr7_4:47839869-47839888 Msa1158440:CDS 25.0%
! TATTGGATAAGATGCTTAAA+GGG + chr7_4:47840127-47840146 None:intergenic 25.0%
! TGAAAAGGAAATTGTGAAAA+AGG - chr7_4:47840274-47840293 Msa1158440:intron 25.0%
! TTTAGGTGAAATTGACTATT+TGG - chr7_4:47840647-47840666 Msa1158440:CDS 25.0%
! TATGAAAGTGAAATGCAATA+TGG + chr7_4:47840790-47840809 None:intergenic 25.0%
AGAGAAAAGAAAGCCAAAAT+TGG + chr7_4:47839355-47839374 None:intergenic 30.0%
AAGGTGTAGAAAAAGTTAAG+GGG + chr7_4:47839425-47839444 None:intergenic 30.0%
! CACTAGAAACTTGTACATTT+TGG + chr7_4:47839465-47839484 None:intergenic 30.0%
!! CACTTCCTACATAATTTGTT+TGG + chr7_4:47839877-47839896 None:intergenic 30.0%
CAAACAAATTATGTAGGAAG+TGG - chr7_4:47839875-47839894 Msa1158440:CDS 30.0%
! GTAAAAGTACACAACTTTGT+GGG + chr7_4:47839950-47839969 None:intergenic 30.0%
!! GTTGTGTTTTGAACATTAGA+GGG + chr7_4:47839972-47839991 None:intergenic 30.0%
!! TGTTGTGTTTTGAACATTAG+AGG + chr7_4:47839973-47839992 None:intergenic 30.0%
!! GACTCATTTTGTTCTTGTTT+TGG + chr7_4:47840035-47840054 None:intergenic 30.0%
GTATTGGATAAGATGCTTAA+AGG + chr7_4:47840128-47840147 None:intergenic 30.0%
!! ATTTTGAGGGTTTAAGGTAT+TGG + chr7_4:47840144-47840163 None:intergenic 30.0%
! AACATAGTGACTCATTTTGA+GGG + chr7_4:47840157-47840176 None:intergenic 30.0%
!!! CAATAAGCTTGTTTTAAGAG+AGG + chr7_4:47840188-47840207 None:intergenic 30.0%
CTCTCTTAAAACAAGCTTAT+TGG - chr7_4:47840186-47840205 Msa1158440:CDS 30.0%
GGTAACAAAAGATTATGAAG+TGG - chr7_4:47840250-47840269 Msa1158440:intron 30.0%
AGATTATGAAGTGGTTGAAA+AGG - chr7_4:47840259-47840278 Msa1158440:intron 30.0%
AGGAAATTGTGAAAAAGGAA+AGG - chr7_4:47840279-47840298 Msa1158440:intron 30.0%
!! AAAGGAAAGGTTTGATTTTG+AGG - chr7_4:47840292-47840311 Msa1158440:intron 30.0%
!! AAGGAAAGGTTTGATTTTGA+GGG - chr7_4:47840293-47840312 Msa1158440:intron 30.0%
!! TTTGAGGTTGATGTTAACAA+TGG - chr7_4:47840317-47840336 Msa1158440:intron 30.0%
TGCAAAAAACTCACAAAAGT+TGG + chr7_4:47840500-47840519 None:intergenic 30.0%
AAATTCACACAAGAAAGTCA+TGG - chr7_4:47840523-47840542 Msa1158440:intron 30.0%
GGAAACATGAGATAGTATTT+AGG - chr7_4:47840668-47840687 Msa1158440:exon 30.0%
GTTGTTAGTGCTATGATTAT+TGG - chr7_4:47840694-47840713 Msa1158440:five_prime_UTR 30.0%
AGAAAGAGACAAAATGAAAC+AGG + chr7_4:47840750-47840769 None:intergenic 30.0%
AGAGAAAAGAAAGCCAAAAT+TGG + chr7_4:47839355-47839374 None:intergenic 30.0%
AAGGTGTAGAAAAAGTTAAG+GGG + chr7_4:47839425-47839444 None:intergenic 30.0%
! CACTAGAAACTTGTACATTT+TGG + chr7_4:47839465-47839484 None:intergenic 30.0%
!! CACTTCCTACATAATTTGTT+TGG + chr7_4:47839877-47839896 None:intergenic 30.0%
CAAACAAATTATGTAGGAAG+TGG - chr7_4:47839875-47839894 Msa1158440:CDS 30.0%
! GTAAAAGTACACAACTTTGT+GGG + chr7_4:47839950-47839969 None:intergenic 30.0%
!! GTTGTGTTTTGAACATTAGA+GGG + chr7_4:47839972-47839991 None:intergenic 30.0%
!! TGTTGTGTTTTGAACATTAG+AGG + chr7_4:47839973-47839992 None:intergenic 30.0%
!! GACTCATTTTGTTCTTGTTT+TGG + chr7_4:47840035-47840054 None:intergenic 30.0%
GTATTGGATAAGATGCTTAA+AGG + chr7_4:47840128-47840147 None:intergenic 30.0%
!! ATTTTGAGGGTTTAAGGTAT+TGG + chr7_4:47840144-47840163 None:intergenic 30.0%
! AACATAGTGACTCATTTTGA+GGG + chr7_4:47840157-47840176 None:intergenic 30.0%
!!! CAATAAGCTTGTTTTAAGAG+AGG + chr7_4:47840188-47840207 None:intergenic 30.0%
CTCTCTTAAAACAAGCTTAT+TGG - chr7_4:47840186-47840205 Msa1158440:CDS 30.0%
GGTAACAAAAGATTATGAAG+TGG - chr7_4:47840250-47840269 Msa1158440:intron 30.0%
AGATTATGAAGTGGTTGAAA+AGG - chr7_4:47840259-47840278 Msa1158440:intron 30.0%
AGGAAATTGTGAAAAAGGAA+AGG - chr7_4:47840279-47840298 Msa1158440:intron 30.0%
!! AAAGGAAAGGTTTGATTTTG+AGG - chr7_4:47840292-47840311 Msa1158440:intron 30.0%
!! AAGGAAAGGTTTGATTTTGA+GGG - chr7_4:47840293-47840312 Msa1158440:intron 30.0%
!! TTTGAGGTTGATGTTAACAA+TGG - chr7_4:47840317-47840336 Msa1158440:intron 30.0%
TGCAAAAAACTCACAAAAGT+TGG + chr7_4:47840500-47840519 None:intergenic 30.0%
AAATTCACACAAGAAAGTCA+TGG - chr7_4:47840523-47840542 Msa1158440:intron 30.0%
GGAAACATGAGATAGTATTT+AGG - chr7_4:47840668-47840687 Msa1158440:exon 30.0%
GTTGTTAGTGCTATGATTAT+TGG - chr7_4:47840694-47840713 Msa1158440:five_prime_UTR 30.0%
AGAAAGAGACAAAATGAAAC+AGG + chr7_4:47840750-47840769 None:intergenic 30.0%
!!! AACTTAGAGAGCACCAATTT+TGG - chr7_4:47839339-47839358 Msa1158440:three_prime_UTR 35.0%
AATTTGAACACACCAAGAGA+GGG + chr7_4:47839395-47839414 None:intergenic 35.0%
TAATTTGAACACACCAAGAG+AGG + chr7_4:47839396-47839415 None:intergenic 35.0%
AGGTGTAGAAAAAGTTAAGG+GGG + chr7_4:47839424-47839443 None:intergenic 35.0%
TTGAAAGGTTAGAGCAAGAA+GGG + chr7_4:47839513-47839532 None:intergenic 35.0%
CATAACTAGGAAGGACTTAT+AGG + chr7_4:47839668-47839687 None:intergenic 35.0%
GTTAGAACAAGGACATAACT+AGG + chr7_4:47839681-47839700 None:intergenic 35.0%
ATTATGTAGGAAGTGGATGT+TGG - chr7_4:47839882-47839901 Msa1158440:CDS 35.0%
! GGTAAAAGTACACAACTTTG+TGG + chr7_4:47839951-47839970 None:intergenic 35.0%
! AAGATGCTTAAAGGGTCTTT+TGG + chr7_4:47840119-47840138 None:intergenic 35.0%
!! TGACTCATTTTGAGGGTTTA+AGG + chr7_4:47840150-47840169 None:intergenic 35.0%
! GAACATAGTGACTCATTTTG+AGG + chr7_4:47840158-47840177 None:intergenic 35.0%
!!! AAGCTTGTTTTAAGAGAGGT+GGG + chr7_4:47840184-47840203 None:intergenic 35.0%
!!! TAAGCTTGTTTTAAGAGAGG+TGG + chr7_4:47840185-47840204 None:intergenic 35.0%
!! AGGAAAGGTTTGATTTTGAG+GGG - chr7_4:47840294-47840313 Msa1158440:intron 35.0%
GTCAAAATCCAATTCCAACA+AGG - chr7_4:47840339-47840358 Msa1158440:intron 35.0%
AAAATCCAATTCCAACAAGG+AGG - chr7_4:47840342-47840361 Msa1158440:intron 35.0%
GTACACATTGTTTGTCACAA+AGG - chr7_4:47840366-47840385 Msa1158440:intron 35.0%
ATGCATTGCATAGTGTCTTT+GGG + chr7_4:47840421-47840440 None:intergenic 35.0%
ACACTACTCACTAGGATTAA+TGG + chr7_4:47840579-47840598 None:intergenic 35.0%
TTAATCCTAGTGAGTAGTGT+TGG - chr7_4:47840579-47840598 Msa1158440:CDS 35.0%
!!! AACTTAGAGAGCACCAATTT+TGG - chr7_4:47839339-47839358 Msa1158440:three_prime_UTR 35.0%
AATTTGAACACACCAAGAGA+GGG + chr7_4:47839395-47839414 None:intergenic 35.0%
TAATTTGAACACACCAAGAG+AGG + chr7_4:47839396-47839415 None:intergenic 35.0%
AGGTGTAGAAAAAGTTAAGG+GGG + chr7_4:47839424-47839443 None:intergenic 35.0%
TTGAAAGGTTAGAGCAAGAA+GGG + chr7_4:47839513-47839532 None:intergenic 35.0%
CATAACTAGGAAGGACTTAT+AGG + chr7_4:47839668-47839687 None:intergenic 35.0%
GTTAGAACAAGGACATAACT+AGG + chr7_4:47839681-47839700 None:intergenic 35.0%
ATTATGTAGGAAGTGGATGT+TGG - chr7_4:47839882-47839901 Msa1158440:CDS 35.0%
! GGTAAAAGTACACAACTTTG+TGG + chr7_4:47839951-47839970 None:intergenic 35.0%
! AAGATGCTTAAAGGGTCTTT+TGG + chr7_4:47840119-47840138 None:intergenic 35.0%
!! TGACTCATTTTGAGGGTTTA+AGG + chr7_4:47840150-47840169 None:intergenic 35.0%
! GAACATAGTGACTCATTTTG+AGG + chr7_4:47840158-47840177 None:intergenic 35.0%
!!! AAGCTTGTTTTAAGAGAGGT+GGG + chr7_4:47840184-47840203 None:intergenic 35.0%
!!! TAAGCTTGTTTTAAGAGAGG+TGG + chr7_4:47840185-47840204 None:intergenic 35.0%
!! AGGAAAGGTTTGATTTTGAG+GGG - chr7_4:47840294-47840313 Msa1158440:intron 35.0%
GTCAAAATCCAATTCCAACA+AGG - chr7_4:47840339-47840358 Msa1158440:intron 35.0%
AAAATCCAATTCCAACAAGG+AGG - chr7_4:47840342-47840361 Msa1158440:intron 35.0%
GTACACATTGTTTGTCACAA+AGG - chr7_4:47840366-47840385 Msa1158440:intron 35.0%
ATGCATTGCATAGTGTCTTT+GGG + chr7_4:47840421-47840440 None:intergenic 35.0%
ACACTACTCACTAGGATTAA+TGG + chr7_4:47840579-47840598 None:intergenic 35.0%
TTAATCCTAGTGAGTAGTGT+TGG - chr7_4:47840579-47840598 Msa1158440:CDS 35.0%
ATTTGAACACACCAAGAGAG+GGG + chr7_4:47839394-47839413 None:intergenic 40.0%
GGTGTAGAAAAAGTTAAGGG+GGG + chr7_4:47839423-47839442 None:intergenic 40.0%
!! GGCACACATCCATTTTGATA+AGG + chr7_4:47839444-47839463 None:intergenic 40.0%
TAGTGAGTGCAAACAAGAGA+AGG - chr7_4:47839480-47839499 Msa1158440:three_prime_UTR 40.0%
CTTGAAAGGTTAGAGCAAGA+AGG + chr7_4:47839514-47839533 None:intergenic 40.0%
! CCTCTTTTCTGCTCAAAACA+CGG - chr7_4:47839546-47839565 Msa1158440:CDS 40.0%
GAACAAGGACATAACTAGGA+AGG + chr7_4:47839677-47839696 None:intergenic 40.0%
AAGTGGATGTTGGAATGGAA+TGG - chr7_4:47839892-47839911 Msa1158440:CDS 40.0%
GGTTTCTTCACTGTGTTTGA+AGG + chr7_4:47840014-47840033 None:intergenic 40.0%
!! AAAGGGTCTTTTGGTCTTCT+TGG + chr7_4:47840110-47840129 None:intergenic 40.0%
! GTTAGCTGATAGTGAACTCA+TGG - chr7_4:47840208-47840227 Msa1158440:CDS 40.0%
TAGTGAACTCATGGTACCTA+AGG - chr7_4:47840217-47840236 Msa1158440:CDS 40.0%
AGTGAACTCATGGTACCTAA+GGG - chr7_4:47840218-47840237 Msa1158440:CDS 40.0%
!! GTTACCTCTTTTTCACCCTT+AGG + chr7_4:47840236-47840255 None:intergenic 40.0%
!! GTTTGATTTTGAGGGGTTTG+AGG - chr7_4:47840301-47840320 Msa1158440:intron 40.0%
ATAGTGTCTTTGGGCCTAAT+GGG + chr7_4:47840412-47840431 None:intergenic 40.0%
CATGCATTGCATAGTGTCTT+TGG + chr7_4:47840422-47840441 None:intergenic 40.0%
AAGACACTATGCAATGCATG+TGG - chr7_4:47840422-47840441 Msa1158440:intron 40.0%
ACAAAAGTTGGACTCTTAGC+AGG + chr7_4:47840488-47840507 None:intergenic 40.0%
ACAAGAAAGTCATGGAGATG+AGG - chr7_4:47840531-47840550 Msa1158440:intron 40.0%
ATATGGTATGCAACATGAGG+AGG + chr7_4:47840773-47840792 None:intergenic 40.0%
GCAATATGGTATGCAACATG+AGG + chr7_4:47840776-47840795 None:intergenic 40.0%
ATTTGAACACACCAAGAGAG+GGG + chr7_4:47839394-47839413 None:intergenic 40.0%
GGTGTAGAAAAAGTTAAGGG+GGG + chr7_4:47839423-47839442 None:intergenic 40.0%
!! GGCACACATCCATTTTGATA+AGG + chr7_4:47839444-47839463 None:intergenic 40.0%
TAGTGAGTGCAAACAAGAGA+AGG - chr7_4:47839480-47839499 Msa1158440:three_prime_UTR 40.0%
CTTGAAAGGTTAGAGCAAGA+AGG + chr7_4:47839514-47839533 None:intergenic 40.0%
! CCTCTTTTCTGCTCAAAACA+CGG - chr7_4:47839546-47839565 Msa1158440:CDS 40.0%
GAACAAGGACATAACTAGGA+AGG + chr7_4:47839677-47839696 None:intergenic 40.0%
AAGTGGATGTTGGAATGGAA+TGG - chr7_4:47839892-47839911 Msa1158440:CDS 40.0%
GGTTTCTTCACTGTGTTTGA+AGG + chr7_4:47840014-47840033 None:intergenic 40.0%
!! AAAGGGTCTTTTGGTCTTCT+TGG + chr7_4:47840110-47840129 None:intergenic 40.0%
! GTTAGCTGATAGTGAACTCA+TGG - chr7_4:47840208-47840227 Msa1158440:CDS 40.0%
TAGTGAACTCATGGTACCTA+AGG - chr7_4:47840217-47840236 Msa1158440:CDS 40.0%
AGTGAACTCATGGTACCTAA+GGG - chr7_4:47840218-47840237 Msa1158440:CDS 40.0%
!! GTTACCTCTTTTTCACCCTT+AGG + chr7_4:47840236-47840255 None:intergenic 40.0%
!! GTTTGATTTTGAGGGGTTTG+AGG - chr7_4:47840301-47840320 Msa1158440:intron 40.0%
ATAGTGTCTTTGGGCCTAAT+GGG + chr7_4:47840412-47840431 None:intergenic 40.0%
CATGCATTGCATAGTGTCTT+TGG + chr7_4:47840422-47840441 None:intergenic 40.0%
AAGACACTATGCAATGCATG+TGG - chr7_4:47840422-47840441 Msa1158440:intron 40.0%
ACAAAAGTTGGACTCTTAGC+AGG + chr7_4:47840488-47840507 None:intergenic 40.0%
ACAAGAAAGTCATGGAGATG+AGG - chr7_4:47840531-47840550 Msa1158440:intron 40.0%
ATATGGTATGCAACATGAGG+AGG + chr7_4:47840773-47840792 None:intergenic 40.0%
GCAATATGGTATGCAACATG+AGG + chr7_4:47840776-47840795 None:intergenic 40.0%
CAAGAGAGGGGAAAATGGTA+AGG + chr7_4:47839382-47839401 None:intergenic 45.0%
! CTTACCATTTTCCCCTCTCT+TGG - chr7_4:47839380-47839399 Msa1158440:three_prime_UTR 45.0%
GGTCATCAAGGCTACTTGAA+AGG + chr7_4:47839528-47839547 None:intergenic 45.0%
GAGCAGAAAAGAGGTCATCA+AGG + chr7_4:47839540-47839559 None:intergenic 45.0%
!! CCGTGTTTTGAGCAGAAAAG+AGG + chr7_4:47839549-47839568 None:intergenic 45.0%
GTAGGAAGTGGATGTTGGAA+TGG - chr7_4:47839887-47839906 Msa1158440:CDS 45.0%
! GAGTCAACTTTTGCAGTTCC+AGG - chr7_4:47840050-47840069 Msa1158440:CDS 45.0%
! CTTTTGCTTCTTGCTTTGCC+TGG + chr7_4:47840071-47840090 None:intergenic 45.0%
CAGGCAAAGCAAGAAGCAAA+AGG - chr7_4:47840069-47840088 Msa1158440:CDS 45.0%
GGTACCTAAGGGTGAAAAAG+AGG - chr7_4:47840229-47840248 Msa1158440:intron 45.0%
GTACACCTCCTTGTTGGAAT+TGG + chr7_4:47840350-47840369 None:intergenic 45.0%
CAATGTGTACACCTCCTTGT+TGG + chr7_4:47840356-47840375 None:intergenic 45.0%
CATAGTGTCTTTGGGCCTAA+TGG + chr7_4:47840413-47840432 None:intergenic 45.0%
TATGCAATGCATGTGGTGTG+AGG - chr7_4:47840429-47840448 Msa1158440:intron 45.0%
TGTGGTGTGAGGTACAAATC+TGG - chr7_4:47840440-47840459 Msa1158440:intron 45.0%
GTGTGAGGTACAAATCTGGT+AGG - chr7_4:47840444-47840463 Msa1158440:intron 45.0%
CTCTTAGCAGGTCTATACTC+TGG + chr7_4:47840476-47840495 None:intergenic 45.0%
! ATCCTAGTGAGTAGTGTTGG+TGG - chr7_4:47840582-47840601 Msa1158440:CDS 45.0%
AACCACCAACACTACTCACT+AGG + chr7_4:47840587-47840606 None:intergenic 45.0%
CAAGAGAGGGGAAAATGGTA+AGG + chr7_4:47839382-47839401 None:intergenic 45.0%
! CTTACCATTTTCCCCTCTCT+TGG - chr7_4:47839380-47839399 Msa1158440:three_prime_UTR 45.0%
GGTCATCAAGGCTACTTGAA+AGG + chr7_4:47839528-47839547 None:intergenic 45.0%
GAGCAGAAAAGAGGTCATCA+AGG + chr7_4:47839540-47839559 None:intergenic 45.0%
!! CCGTGTTTTGAGCAGAAAAG+AGG + chr7_4:47839549-47839568 None:intergenic 45.0%
GTAGGAAGTGGATGTTGGAA+TGG - chr7_4:47839887-47839906 Msa1158440:CDS 45.0%
! GAGTCAACTTTTGCAGTTCC+AGG - chr7_4:47840050-47840069 Msa1158440:CDS 45.0%
! CTTTTGCTTCTTGCTTTGCC+TGG + chr7_4:47840071-47840090 None:intergenic 45.0%
CAGGCAAAGCAAGAAGCAAA+AGG - chr7_4:47840069-47840088 Msa1158440:CDS 45.0%
GGTACCTAAGGGTGAAAAAG+AGG - chr7_4:47840229-47840248 Msa1158440:intron 45.0%
GTACACCTCCTTGTTGGAAT+TGG + chr7_4:47840350-47840369 None:intergenic 45.0%
CAATGTGTACACCTCCTTGT+TGG + chr7_4:47840356-47840375 None:intergenic 45.0%
CATAGTGTCTTTGGGCCTAA+TGG + chr7_4:47840413-47840432 None:intergenic 45.0%
TATGCAATGCATGTGGTGTG+AGG - chr7_4:47840429-47840448 Msa1158440:intron 45.0%
TGTGGTGTGAGGTACAAATC+TGG - chr7_4:47840440-47840459 Msa1158440:intron 45.0%
GTGTGAGGTACAAATCTGGT+AGG - chr7_4:47840444-47840463 Msa1158440:intron 45.0%
CTCTTAGCAGGTCTATACTC+TGG + chr7_4:47840476-47840495 None:intergenic 45.0%
! ATCCTAGTGAGTAGTGTTGG+TGG - chr7_4:47840582-47840601 Msa1158440:CDS 45.0%
AACCACCAACACTACTCACT+AGG + chr7_4:47840587-47840606 None:intergenic 45.0%
CACACCAAGAGAGGGGAAAA+TGG + chr7_4:47839387-47839406 None:intergenic 50.0%
TGTCACAAAGGACCCCACAA+TGG - chr7_4:47840378-47840397 Msa1158440:intron 50.0%
ACAAAGGACCCCACAATGGA+GGG - chr7_4:47840382-47840401 Msa1158440:intron 50.0%
CACACCAAGAGAGGGGAAAA+TGG + chr7_4:47839387-47839406 None:intergenic 50.0%
TGTCACAAAGGACCCCACAA+TGG - chr7_4:47840378-47840397 Msa1158440:intron 50.0%
ACAAAGGACCCCACAATGGA+GGG - chr7_4:47840382-47840401 Msa1158440:intron 50.0%
CACAAAGGACCCCACAATGG+AGG - chr7_4:47840381-47840400 Msa1158440:intron 55.0%
CACAAAGGACCCCACAATGG+AGG - chr7_4:47840381-47840400 Msa1158440:intron 55.0%
ATGGGCCTGCCCTCCATTGT+GGG + chr7_4:47840394-47840413 None:intergenic 60.0%
AATGGGCCTGCCCTCCATTG+TGG + chr7_4:47840395-47840414 None:intergenic 60.0%
CAATGGAGGGCAGGCCCATT+AGG - chr7_4:47840395-47840414 Msa1158440:intron 60.0%
ATGGGCCTGCCCTCCATTGT+GGG + chr7_4:47840394-47840413 None:intergenic 60.0%
AATGGGCCTGCCCTCCATTG+TGG + chr7_4:47840395-47840414 None:intergenic 60.0%
CAATGGAGGGCAGGCCCATT+AGG - chr7_4:47840395-47840414 Msa1158440:intron 60.0%
AGGACCCCACAATGGAGGGC+AGG - chr7_4:47840386-47840405 Msa1158440:intron 65.0%
TGGGCCTGCCCTCCATTGTG+GGG + chr7_4:47840393-47840412 None:intergenic 65.0%
AGGACCCCACAATGGAGGGC+AGG - chr7_4:47840386-47840405 Msa1158440:intron 65.0%
TGGGCCTGCCCTCCATTGTG+GGG + chr7_4:47840393-47840412 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 47839313 47840820 47839313 ID=Msa1158440;Name=Msa1158440
chr7_4 mRNA 47839313 47840820 47839313 ID=Msa1158440-mRNA-1;Parent=Msa1158440;Name=Msa1158440-mRNA-1;_AED=0.01;_eAED=0.01;_QI=138|1|1|1|1|1|2|225|273
chr7_4 exon 47839313 47840242 47839313 ID=Msa1158440-mRNA-1:exon:12794;Parent=Msa1158440-mRNA-1
chr7_4 exon 47840566 47840820 47840566 ID=Msa1158440-mRNA-1:exon:12793;Parent=Msa1158440-mRNA-1
chr7_4 five_prime_UTR 47840683 47840820 47840683 ID=Msa1158440-mRNA-1:five_prime_utr;Parent=Msa1158440-mRNA-1
chr7_4 CDS 47840566 47840682 47840566 ID=Msa1158440-mRNA-1:cds;Parent=Msa1158440-mRNA-1
chr7_4 CDS 47839538 47840242 47839538 ID=Msa1158440-mRNA-1:cds;Parent=Msa1158440-mRNA-1
chr7_4 three_prime_UTR 47839313 47839537 47839313 ID=Msa1158440-mRNA-1:three_prime_utr;Parent=Msa1158440-mRNA-1
Gene Sequence

>Msa1158440

ATGGATGTGTGCCAAAATGTACAAGTTTCTAGTGAGTGCAAACAAGAGAAGGTTCTTAACCCTTCTTGCTCTAACCTTTCAAGTAGCCTTGATGACCTCTTTTCTGCTCAAAACACGGAAGTGGATGTTGGAATGGAATGGCTTTCAGTGTTTGTTGAAGACTGTTTTTCAAGCCCACAAAGTTGTGTACTTTTACCCTCTAATGTTCAAAACACAACAAGTACTGTCTCATCAAAACCTTCAAACACAGTGAAGAAACCAAAACAAGAACAAAATGAGTCAACTTTTGCAGTTCCAGGCAAAGCAAGAAGCAAAAGGAAGAGACTTTCAGCTCCAAGAAGACCAAAAGACCCTTTAAGCATCTTATCCAATACCTTAAACCCTCAAAATGAGTCACTATGTTCTGACCCACCTCTCTTAAAACAAGCTTATTGGTTAGCTGATAGTGAACTCATGGTACCTAAGGGTGAAAAAGAGGTAACAAAAGATTATGAAGTGGTTGAAAAGGAAATTGTGAAAAAGGAAAGGTTTGATTTTGAGGGGTTTGAGGTTGATGTTAACAATGGTCAAAATCCAATTCCAACAAGGAGGTGTACACATTGTTTGTCACAAAGGACCCCACAATGGAGGGCAGGCCCATTAGGCCCAAAGACACTATGCAATGCATGTGGTGTGAGGTACAAATCTGGTAGGTTGTTGCCAGAGTATAGACCTGCTAAGAGTCCAACTTTTGTGAGTTTTTTGCATTCAAATTCACACAAGAAAGTCATGGAGATGAGGATGAATGTGAATGTTGTATCTTCCATTAATCCTAGTGAGTAG
Protein sequence

>Msa1158440

MDVCQNVQVSSECKQEKVLNPSCSNLSSSLDDLFSAQNTEVDVGMEWLSVFVEDCFSSPQSCVLLPSNVQNTTSTVSSKPSNTVKKPKQEQNESTFAVPGKARSKRKRLSAPRRPKDPLSILSNTLNPQNESLCSDPPLLKQAYWLADSELMVPKGEKEVTKDYEVVEKEIVKKERFDFEGFEVDVNNGQNPIPTRRCTHCLSQRTPQWRAGPLGPKTLCNACGVRYKSGRLLPEYRPAKSPTFVSFLHSNSHKKVMEMRMNVNVVSSINPSE*