AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0158600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158600 MtrunA17_Chr1g0170651 79.789 569 48 7 2 517 8 562 0.0 858
Msa0158600 MtrunA17_Chr5g0399801 37.064 545 229 16 35 505 576 1080 1.37e-89 296
Msa0158600 MtrunA17_Chr4g0003501 36.248 549 232 19 39 511 448 954 2.33e-85 283
Msa0158600 MtrunA17_Chr4g0003511 35.701 549 237 16 39 511 543 1051 1.27e-83 280
Msa0158600 MtrunA17_Chr8g0354821 32.417 509 239 17 42 488 191 656 8.76e-58 204
Msa0158600 MtrunA17_Chr7g0255181 31.772 491 246 19 65 505 245 696 9.20e-57 201
Msa0158600 MtrunA17_Chr3g0080761 30.039 516 252 17 38 488 161 632 1.64e-55 197
Msa0158600 MtrunA17_Chr6g0476121 30.392 510 237 16 75 505 164 634 9.88e-55 194
Msa0158600 MtrunA17_Chr6g0476111 30.196 510 238 16 75 505 215 685 4.57e-54 193
Msa0158600 MtrunA17_Chr7g0232981 29.928 558 243 18 4 504 40 506 1.40e-53 189
Msa0158600 MtrunA17_Chr7g0252211 30.375 507 240 17 76 505 278 748 8.07e-53 191
Msa0158600 MtrunA17_Chr8g0368901 31.743 482 241 16 64 488 305 755 2.20e-52 190
Msa0158600 MtrunA17_Chr8g0369301 30.916 524 262 21 25 488 252 735 2.44e-52 189
Msa0158600 MtrunA17_Chr8g0368911 30.083 482 251 15 63 488 297 748 3.65e-52 189
Msa0158600 MtrunA17_Chr1g0163051 31.942 479 239 16 65 488 198 644 5.79e-52 187
Msa0158600 MtrunA17_Chr7g0232811 29.690 549 238 16 25 505 62 530 1.28e-51 184
Msa0158600 MtrunA17_Chr1g0163041 29.876 482 247 20 65 488 115 563 3.15e-45 167
Msa0158600 MtrunA17_Chr6g0467531 29.936 471 234 17 76 488 130 562 3.26e-43 161
Msa0158600 MtrunA17_Chr7g0232841 28.829 555 221 20 25 505 65 519 3.64e-43 160
Msa0158600 MtrunA17_Chr6g0467421 30.316 475 227 18 76 488 130 562 5.80e-43 160
Msa0158600 MtrunA17_Chr6g0467381 30.526 475 226 18 76 488 130 562 6.60e-43 160
Msa0158600 MtrunA17_Chr6g0467401 30.316 475 227 18 76 488 130 562 2.09e-41 156
Msa0158600 MtrunA17_Chr6g0467411 27.290 524 250 18 25 488 28 480 1.42e-37 144
Msa0158600 MtrunA17_Chr3g0094881 27.409 467 249 14 76 488 191 621 1.77e-34 137
Msa0158600 MtrunA17_Chr5g0403241 31.230 317 149 10 240 488 1168 1483 7.60e-29 121
Msa0158600 MtrunA17_Chr8g0373821 30.292 274 134 9 271 489 245 516 2.79e-26 112
Msa0158600 MtrunA17_Chr4g0075321 36.782 174 88 5 337 489 869 1041 3.26e-25 110
Msa0158600 MtrunA17_Chr2g0306961 29.333 225 119 7 301 488 240 461 1.20e-21 97.8
Msa0158600 MtrunA17_Chr2g0306961 44.037 109 44 3 76 175 79 179 4.96e-12 68.2
Msa0158600 MtrunA17_Chr1g0190311 28.475 295 126 12 276 488 325 616 2.51e-20 94.7
Msa0158600 MtrunA17_Chr1g0183321 27.740 292 131 12 276 488 428 718 9.43e-19 89.7
Msa0158600 MtrunA17_Chr1g0201311 40.268 149 66 7 356 489 61 201 1.45e-18 88.6
Msa0158600 MtrunA17_Chr7g0232751 44.578 83 41 1 428 505 28 110 4.96e-18 80.1
Msa0158600 MtrunA17_Chr1g0201621 39.597 149 67 7 356 489 61 201 7.59e-18 86.3
Msa0158600 MtrunA17_Chr7g0262001 26.441 295 141 11 270 488 380 674 2.93e-16 82.0
Msa0158600 MtrunA17_Chr2g0305371 32.057 209 103 9 292 488 268 449 3.68e-15 78.2
Msa0158600 MtrunA17_Chr3g0125251 32.692 156 90 5 360 505 1306 1456 7.15e-14 75.1
Msa0158600 MtrunA17_Chr1g0149611 37.594 133 54 5 373 489 365 484 1.44e-13 73.2
Msa0158600 MtrunA17_Chr7g0232861 34.857 175 89 7 25 182 61 227 2.86e-13 69.7
Msa0158600 MtrunA17_Chr7g0232761 32.787 183 97 7 40 207 94 265 1.20e-12 68.6
Msa0158600 MtrunA17_Chr7g0232871 47.368 57 28 1 370 426 22 76 2.82e-12 63.5
Msa0158600 MtrunA17_Chr1g0149581 33.333 144 74 7 373 504 1523 1656 2.55e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158600 AT2G35160.5 39.549 488 227 18 57 511 305 757 1.40e-88 288
Msa0158600 AT2G35160.2 39.549 488 227 18 57 511 341 793 3.46e-88 288
Msa0158600 AT2G35160.3 39.549 488 227 18 57 511 341 793 3.46e-88 288
Msa0158600 AT2G35160.1 39.549 488 227 18 57 511 341 793 3.46e-88 288
Msa0158600 AT2G35160.4 39.549 488 227 18 57 511 341 793 3.46e-88 288
Msa0158600 AT2G22740.2 34.953 535 237 18 41 510 298 786 9.07e-75 252
Msa0158600 AT2G22740.1 34.953 535 237 18 41 510 298 786 9.07e-75 252
Msa0158600 AT1G73100.1 33.192 473 222 16 73 488 202 637 2.05e-60 211
Msa0158600 AT5G04940.2 32.794 494 234 19 73 505 205 661 5.98e-56 199
Msa0158600 AT5G04940.1 32.794 494 234 19 73 505 205 661 5.98e-56 199
Msa0158600 AT2G33290.1 30.143 491 262 17 64 504 191 650 3.68e-49 179
Msa0158600 AT2G33290.3 30.143 491 262 17 64 504 191 650 3.68e-49 179
Msa0158600 AT2G33290.4 30.143 491 262 17 64 504 191 650 3.68e-49 179
Msa0158600 AT2G33290.2 30.143 491 262 17 64 504 191 650 3.68e-49 179
Msa0158600 AT5G13960.2 31.014 503 241 19 76 505 12 481 6.25e-47 171
Msa0158600 AT4G13460.1 30.063 479 236 17 71 488 196 636 2.27e-46 172
Msa0158600 AT4G13460.2 30.063 479 236 17 71 488 196 636 2.27e-46 172
Msa0158600 AT5G13960.1 31.014 503 241 19 76 505 146 615 2.37e-46 171
Msa0158600 AT1G17770.1 29.109 505 245 21 73 505 221 684 4.13e-43 163
Msa0158600 AT2G24740.1 29.583 480 254 15 73 505 304 746 1.64e-40 156
Msa0158600 AT2G23740.1 33.448 290 133 11 252 488 1066 1348 4.25e-31 129
Msa0158600 AT2G23740.3 33.448 290 133 11 252 488 1073 1355 4.83e-31 128
Msa0158600 AT2G23740.6 33.448 290 133 11 252 488 1073 1355 4.83e-31 128
Msa0158600 AT2G23740.2 33.448 290 133 11 252 488 1073 1355 4.83e-31 128
Msa0158600 AT5G47150.1 45.000 140 61 3 73 205 170 300 1.11e-23 102
Msa0158600 AT5G47160.1 44.737 152 69 3 73 217 256 399 2.05e-23 102
Msa0158600 AT5G47160.3 44.737 152 69 3 73 217 256 399 2.05e-23 102
Msa0158600 AT5G47160.2 44.737 152 69 3 73 217 256 399 2.05e-23 102
Msa0158600 AT2G23740.4 31.325 249 111 11 252 447 1073 1314 2.59e-19 92.4
Msa0158600 AT2G23740.5 31.325 249 111 11 252 447 1073 1314 2.59e-19 92.4
Msa0158600 AT3G04380.3 24.876 402 187 19 183 488 36 418 8.41e-18 86.7
Msa0158600 AT3G04380.4 24.876 402 187 19 183 488 52 434 9.46e-18 86.7
Msa0158600 AT3G04380.1 24.876 402 187 19 183 488 52 434 1.12e-17 86.3
Msa0158600 AT3G03750.2 32.995 197 92 7 314 488 144 322 4.28e-17 83.2
Msa0158600 AT5G43990.9 32.979 188 104 7 349 515 544 730 4.38e-17 85.1
Msa0158600 AT5G43990.2 32.979 188 104 7 349 515 544 730 4.38e-17 85.1
Msa0158600 AT5G43990.16 32.979 188 104 7 349 515 501 687 4.41e-17 85.1
Msa0158600 AT5G43990.5 32.979 188 104 7 349 515 501 687 4.41e-17 85.1
Msa0158600 AT5G43990.17 32.979 188 104 7 349 515 501 687 4.41e-17 85.1
Msa0158600 AT5G43990.14 32.979 188 104 7 349 515 501 687 4.41e-17 85.1
Msa0158600 AT5G43990.3 32.979 188 104 7 349 515 521 707 4.51e-17 85.1
Msa0158600 AT5G43990.11 32.979 188 104 7 349 515 521 707 4.51e-17 85.1
Msa0158600 AT5G43990.1 32.979 188 104 7 349 515 521 707 4.51e-17 85.1
Msa0158600 AT5G43990.4 32.979 188 104 7 349 515 521 707 4.51e-17 85.1
Msa0158600 AT5G43990.15 32.979 188 104 7 349 515 524 710 4.56e-17 85.1
Msa0158600 AT5G43990.10 32.979 188 104 7 349 515 524 710 4.56e-17 85.1
Msa0158600 AT5G43990.7 32.979 188 104 7 349 515 432 618 4.86e-17 84.7
Msa0158600 AT5G43990.8 32.979 188 104 7 349 515 452 638 6.36e-17 84.3
Msa0158600 AT5G43990.12 32.979 188 104 7 349 515 452 638 6.36e-17 84.3
Msa0158600 AT5G43990.6 32.979 188 104 7 349 515 452 638 6.36e-17 84.3
Msa0158600 AT5G43990.13 32.979 188 104 7 349 515 452 638 6.36e-17 84.3
Msa0158600 AT3G04380.2 26.045 311 146 13 259 488 100 407 1.21e-16 82.8
Msa0158600 AT3G04380.6 26.045 311 146 13 259 488 100 407 1.36e-16 82.8
Msa0158600 AT3G04380.5 26.045 311 146 13 259 488 84 391 1.40e-16 82.4
Msa0158600 AT1G04050.2 33.333 159 87 5 349 488 537 695 2.71e-16 82.4
Msa0158600 AT1G04050.1 33.333 159 87 5 349 488 537 695 2.71e-16 82.4
Msa0158600 AT1G04050.3 33.333 159 87 5 349 488 433 591 2.95e-16 82.4
Msa0158600 AT1G77300.2 30.968 155 96 4 357 505 1009 1158 2.52e-14 76.6
Msa0158600 AT1G77300.1 30.968 155 96 4 357 505 1009 1158 2.56e-14 76.6
Msa0158600 AT4G30860.1 36.429 140 77 4 357 488 307 442 6.04e-14 74.7
Msa0158600 AT3G59960.3 32.432 148 78 4 372 510 112 246 3.38e-12 67.8
Msa0158600 AT3G59960.2 32.432 148 78 4 372 510 112 246 3.38e-12 67.8
Msa0158600 AT3G59960.4 32.432 148 78 4 372 510 112 246 3.38e-12 67.8
Msa0158600 AT3G03750.1 31.937 191 86 8 314 488 144 306 6.35e-12 67.4
Msa0158600 AT3G59960.1 32.432 148 78 4 372 510 112 246 6.56e-12 67.4

Find 133 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGATTTAAATGTCTAATT+AGG 0.061614 1_4:+28154896 Msa0158600:three_prime_UTR
CTCATGTGGTATCGATTATT+TGG 0.102559 1_4:+28152087 Msa0158600:CDS
AATTATGTAATAGTATAATT+AGG 0.173935 1_4:+28155027 Msa0158600:three_prime_UTR
TACATGCCCTATTCTCTTTC+CGG 0.210201 1_4:-28151993 None:intergenic
TGATGACTATATAATTAATA+TGG 0.212544 1_4:+28153119 Msa0158600:CDS
ATGCAAAGTTCAAAATCTTT+TGG 0.219974 1_4:+28151737 Msa0158600:CDS
TGGGCGCTTACTGAAGAATT+TGG 0.224785 1_4:+28154839 Msa0158600:CDS
GCGACACCGTCCCTTATATT+AGG 0.242119 1_4:-28152214 None:intergenic
CTTCAGAATTGTGTCTCTTC+TGG 0.280628 1_4:-28152516 None:intergenic
TCCTGTTAGGGTTATATTGC+AGG 0.288637 1_4:+28152300 Msa0158600:CDS
CTGATTTAAATGTCTAATTA+GGG 0.295750 1_4:+28154897 Msa0158600:three_prime_UTR
GTTCGGCCCTATATTGAAAT+TGG 0.307938 1_4:-28152036 None:intergenic
TACCTTTAGTATAATCCTAT+TGG 0.309534 1_4:+28155066 Msa0158600:three_prime_UTR
AGTATTGAACGATTCGTCTT+TGG 0.310118 1_4:-28152754 None:intergenic
TAATTAGTACCCCTCGAAAA+AGG 0.317377 1_4:+28154999 Msa0158600:three_prime_UTR
GTATCGATTATTTGGGTAAA+GGG 0.327727 1_4:+28152095 Msa0158600:CDS
TGTTCGAATCAATGATATTT+CGG 0.328396 1_4:+28152639 Msa0158600:CDS
ACTAGGTAACCGAAGGGTTT+AGG 0.329178 1_4:-28152718 None:intergenic
TGTTTCCCCTCGAGATGTTA+TGG 0.333335 1_4:+28152486 Msa0158600:CDS
CGCGCCTCATGATCAGAAAT+TGG 0.347176 1_4:+28152234 Msa0158600:CDS
ACTCGAGTAAGAGGACAATT+TGG 0.352436 1_4:+28152412 Msa0158600:CDS
TGGTGACCAATTTCAATATA+GGG 0.354159 1_4:+28152030 Msa0158600:CDS
GATCGAATATGTTTACCAAT+AGG 0.355001 1_4:-28155081 None:intergenic
CGCGCGGAAATCTTGCTTTG+TGG 0.366478 1_4:+28152257 Msa0158600:CDS
ATGGTGGTAACCCTAATATA+AGG 0.370183 1_4:+28152203 Msa0158600:CDS
ATAGGTGTCACCACCCGAAT+AGG 0.370547 1_4:-28152679 None:intergenic
ACCTGCAATATAACCCTAAC+AGG 0.380684 1_4:-28152301 None:intergenic
GGGTAAAGGGGAGGGTTCTT+TGG 0.380696 1_4:+28152108 Msa0158600:CDS
ATTATGTAATAGTATAATTA+GGG 0.380888 1_4:+28155028 Msa0158600:three_prime_UTR
TGAATGCGGGGAGTCCTGTT+AGG 0.386175 1_4:+28152287 Msa0158600:CDS
GCAAAGAAAAGTTCCCTATT+CGG 0.386328 1_4:+28152665 Msa0158600:CDS
CGACACCGTCCCTTATATTA+GGG 0.395566 1_4:-28152213 None:intergenic
TCTTATTCATACATATATGA+TGG 0.403540 1_4:+28152367 Msa0158600:CDS
TTGGTGACCAATTTCAATAT+AGG 0.405625 1_4:+28152029 Msa0158600:CDS
CAAAGAAAAGTTCCCTATTC+GGG 0.406699 1_4:+28152666 Msa0158600:CDS
GTTTCCCCTCGAGATGTTAT+GGG 0.409974 1_4:+28152487 Msa0158600:CDS
GCGAATGTTATGAAGTCTTC+TGG 0.411419 1_4:+28152160 Msa0158600:CDS
TACCAATAGGATTATACTAA+AGG 0.429034 1_4:-28155068 None:intergenic
TCATGTGGTATCGATTATTT+GGG 0.431263 1_4:+28152088 Msa0158600:CDS
AAGACGAATCGTTCAATACT+AGG 0.431282 1_4:+28152757 Msa0158600:CDS
ACATCTTTCACAACTAGGTT+AGG 0.431941 1_4:-28153311 None:intergenic
CAAAATGTTGTTTGCTACAA+AGG 0.440548 1_4:+28153367 Msa0158600:CDS
GAGCTCACACACAAAGCTTC+CGG 0.447492 1_4:-28153043 None:intergenic
GAAAACACCGGAAAGAGAAT+AGG 0.449177 1_4:+28151986 Msa0158600:CDS
TTTGTTGATTAACACGGGCT+GGG 0.451402 1_4:+28154932 Msa0158600:three_prime_UTR
AGGGCCGAACTTATTGTAAT+CGG 0.452930 1_4:+28152049 Msa0158600:CDS
ATTATGTACACCCTAATTGC+TGG 0.458743 1_4:-28154873 None:intergenic
AAAACACCGGAAAGAGAATA+GGG 0.465297 1_4:+28151987 Msa0158600:CDS
GATTATTTGGGTAAAGGGGA+GGG 0.467741 1_4:+28152100 Msa0158600:CDS
ATTTGTTGATTAACACGGGC+TGG 0.471045 1_4:+28154931 Msa0158600:three_prime_UTR
AAGAGAATAGGGCATGTAGA+TGG 0.472138 1_4:+28151998 Msa0158600:CDS
AAACATATTCGATCAATGCC+TGG 0.474494 1_4:+28155090 Msa0158600:three_prime_UTR
GGTATCGATTATTTGGGTAA+AGG 0.478346 1_4:+28152094 Msa0158600:CDS
GACAATGTGACTCGAGTAAG+AGG 0.478502 1_4:+28152403 Msa0158600:CDS
TTATGAAGGCGAAACGTCAA+TGG 0.484134 1_4:+28151963 Msa0158600:CDS
GATGACTATATAATTAATAT+GGG 0.486512 1_4:+28153120 Msa0158600:CDS
TAGGTGTCACCACCCGAATA+GGG 0.488457 1_4:-28152678 None:intergenic
TACCACATGAGTGTTGTTGA+TGG 0.494297 1_4:-28152075 None:intergenic
TAAGAAAATTCAGGACCAAC+CGG 0.497017 1_4:+28152459 Msa0158600:intron
CTTATTCATACATATATGAT+GGG 0.498680 1_4:+28152368 Msa0158600:CDS
ATTGATTCGAACAACATCCT+TGG 0.502629 1_4:-28152629 None:intergenic
CGATTATTTGGGTAAAGGGG+AGG 0.503266 1_4:+28152099 Msa0158600:CDS
AGCTTTGTGTGTGAGCTCAT+TGG 0.503294 1_4:+28153048 Msa0158600:CDS
TTGTTGATTAACACGGGCTG+GGG 0.505839 1_4:+28154933 Msa0158600:three_prime_UTR
ACATAACATCTTTCACAACT+AGG 0.508844 1_4:-28153316 None:intergenic
ATGAAGAACACAAGCAAGAA+GGG 0.514647 1_4:-28152918 None:intergenic
ATCTTCAAAACAAAATTGAA+AGG 0.515011 1_4:+28152988 Msa0158600:CDS
CAAAACAAAATTGAAAGGGT+GGG 0.521314 1_4:+28152993 Msa0158600:CDS
AACATATTCGATCAATGCCT+GGG 0.522306 1_4:+28155091 Msa0158600:three_prime_UTR
TTGGGAGAAAGCGTGAAGAA+TGG 0.523029 1_4:+28151756 Msa0158600:CDS
GCAACTAGACATGGAAACAT+TGG 0.525331 1_4:+28153263 Msa0158600:CDS
ACACGCTCTTTCATTCCATC+CGG 0.526296 1_4:+28153024 Msa0158600:CDS
TGGTGGTAACCCTAATATAA+GGG 0.526528 1_4:+28152204 Msa0158600:CDS
TCAAAACAAAATTGAAAGGG+TGG 0.528168 1_4:+28152992 Msa0158600:CDS
AAACATTCTTTGGGAGAACA+TGG 0.531170 1_4:-28152540 None:intergenic
CATGTAGATGGAATTGAAGT+TGG 0.532327 1_4:+28152010 Msa0158600:CDS
TTGAGTAAAATTTGCTACTG+TGG 0.533623 1_4:+28153452 Msa0158600:CDS
ATTTATTTGTTGATTAACAC+GGG 0.540345 1_4:+28154927 Msa0158600:three_prime_UTR
GATGAAGAACACAAGCAAGA+AGG 0.541031 1_4:-28152919 None:intergenic
GAATGCGGGGAGTCCTGTTA+GGG 0.541817 1_4:+28152288 Msa0158600:CDS
TCTTCAAAACAAAATTGAAA+GGG 0.541871 1_4:+28152989 Msa0158600:CDS
GAGACCGATTACAATAAGTT+CGG 0.543201 1_4:-28152053 None:intergenic
CGAAGCTACAAGGTTTATGA+AGG 0.543860 1_4:+28151949 Msa0158600:intron
TTGCTGCAAGGTGAATATCA+AGG 0.545402 1_4:+28153427 Msa0158600:CDS
AAAACAAAATTGAAAGGGTG+GGG 0.546151 1_4:+28152994 Msa0158600:CDS
CGCGCCAATTTCTGATCATG+AGG 0.548143 1_4:-28152238 None:intergenic
TATGAAGGCGAAACGTCAAT+GGG 0.552062 1_4:+28151964 Msa0158600:CDS
TTCATTGATGCAACTAGACA+TGG 0.553236 1_4:+28153254 Msa0158600:CDS
TAGAGGTCTTGTTCTCTCCA+AGG 0.558562 1_4:+28152612 Msa0158600:CDS
TCTATGAGTTACAACAATGC+TGG 0.561265 1_4:-28152134 None:intergenic
TATATAACGGATGATTTCAG+AGG 0.567575 1_4:-28152886 None:intergenic
GTGGAGATACCTAAACCCTT+CGG 0.568688 1_4:+28152709 Msa0158600:intron
TATTTATTTGTTGATTAACA+CGG 0.568775 1_4:+28154926 Msa0158600:three_prime_UTR
TTATCTTGTCAGAAGATGAA+AGG 0.571853 1_4:-28151793 None:intergenic
GGTGGGGCATTAGCCTATGA+TGG 0.574925 1_4:+28152844 Msa0158600:CDS
TTGGTTTCCATAACATCTCG+AGG 0.577098 1_4:-28152565 None:intergenic
CGAATCAATGATATTTCGGA+GGG 0.577253 1_4:+28152643 Msa0158600:CDS
CAGAAGAGACACAATTCTGA+AGG 0.581158 1_4:+28152517 Msa0158600:CDS
TAACTCAGCTCTCTACCTGC+GGG 0.583390 1_4:-28153395 None:intergenic
AGGGCCACACTCATATATAA+CGG 0.584525 1_4:-28152899 None:intergenic
AATCGTTCAATACTAGGTGG+TGG 0.591568 1_4:+28152763 Msa0158600:CDS
TTGTGGAATAGTATGAATGC+GGG 0.595742 1_4:+28152274 Msa0158600:CDS
ATATAACGGATGATTTCAGA+GGG 0.600456 1_4:-28152885 None:intergenic
CATATTGACTAGGTAACCGA+AGG 0.601280 1_4:-28152725 None:intergenic
CTAGACATGGAAACATTGGA+AGG 0.602878 1_4:+28153267 Msa0158600:CDS
TTTGCATAGCTAAGAATGGT+GGG 0.609720 1_4:+28152827 Msa0158600:CDS
TCTCGAGGGGAAACACTAGC+CGG 0.615076 1_4:-28152478 None:intergenic
TGTGTTTGCATAGCTAAGAA+TGG 0.620233 1_4:+28152823 Msa0158600:CDS
TTGGATAAGTCATATTGACT+AGG 0.621126 1_4:-28152735 None:intergenic
AAAGTTCCCATAACATCTCG+AGG 0.623289 1_4:-28152493 None:intergenic
TATCGATTATTTGGGTAAAG+GGG 0.626149 1_4:+28152096 Msa0158600:CDS
TCGAATCAATGATATTTCGG+AGG 0.627900 1_4:+28152642 Msa0158600:CDS
GAGGGGAAACACTAGCCGGT+TGG 0.631704 1_4:-28152474 None:intergenic
ATAACTCAGCTCTCTACCTG+CGG 0.634593 1_4:-28153396 None:intergenic
AAGTTCCCATAACATCTCGA+GGG 0.638178 1_4:-28152492 None:intergenic
GCTACAAAGGACATACCCGC+AGG 0.652394 1_4:+28153380 Msa0158600:CDS
GGTAACCCTAATATAAGGGA+CGG 0.664705 1_4:+28152208 Msa0158600:CDS
CTCCATCAACAACACTCATG+TGG 0.665071 1_4:+28152073 Msa0158600:CDS
TCATCCGTTATATATGAGTG+TGG 0.671397 1_4:+28152895 Msa0158600:CDS
AGTTCCCATAACATCTCGAG+GGG 0.674068 1_4:-28152491 None:intergenic
ATATTGACTAGGTAACCGAA+GGG 0.676746 1_4:-28152724 None:intergenic
TCACACACAAAGCTTCCGGA+TGG 0.681981 1_4:-28153039 None:intergenic
AGAAAAGTTCCCTATTCGGG+TGG 0.682829 1_4:+28152669 Msa0158600:CDS
AAACGTCAATGGGAAAACAC+CGG 0.685237 1_4:+28151974 Msa0158600:CDS
TGTGGAATAGTATGAATGCG+GGG 0.700340 1_4:+28152275 Msa0158600:CDS
CATGATCAGAAATTGGCGCG+CGG 0.700635 1_4:+28152241 Msa0158600:CDS
TTTGTGGAATAGTATGAATG+CGG 0.704358 1_4:+28152273 Msa0158600:CDS
TTGCTTTATGAAGGTCATGG+TGG 0.708605 1_4:+28152187 Msa0158600:intron
GTTTGCATAGCTAAGAATGG+TGG 0.719383 1_4:+28152826 Msa0158600:CDS
TTGCATAGCTAAGAATGGTG+GGG 0.720544 1_4:+28152828 Msa0158600:CDS
TTATGATTACAATTGCTGCA+AGG 0.726923 1_4:+28153415 Msa0158600:CDS
ACGAATCGTTCAATACTAGG+TGG 0.736474 1_4:+28152760 Msa0158600:CDS
TATAACGGATGATTTCAGAG+GGG 0.813910 1_4:-28152884 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATACATTAGTTAAAAAATA+TGG - chr1_4:28154453-28154472 None:intergenic 10.0%
!! TAGATAATTAAAGATATTAA+CGG + chr1_4:28154556-28154575 Msa0158600:intron 10.0%
!! AATTATGTAATAGTATAATT+AGG + chr1_4:28155027-28155046 Msa0158600:three_prime_UTR 10.0%
!! ATTATGTAATAGTATAATTA+GGG + chr1_4:28155028-28155047 Msa0158600:three_prime_UTR 10.0%
!!! ATAAAGCATTAACAATTTTT+AGG + chr1_4:28151849-28151868 Msa0158600:CDS 15.0%
!!! TAAAGCATTAACAATTTTTA+GGG + chr1_4:28151850-28151869 Msa0158600:CDS 15.0%
!! TAAAATTGAAAGCAAAATTA+AGG + chr1_4:28151918-28151937 Msa0158600:intron 15.0%
!! AAAATTGAAAGCAAAATTAA+GGG + chr1_4:28151919-28151938 Msa0158600:intron 15.0%
!! TGATGACTATATAATTAATA+TGG + chr1_4:28153119-28153138 Msa0158600:CDS 15.0%
!! GATGACTATATAATTAATAT+GGG + chr1_4:28153120-28153139 Msa0158600:CDS 15.0%
!!! TATTTATTTGTTGATTAACA+CGG + chr1_4:28154926-28154945 Msa0158600:three_prime_UTR 15.0%
!!! AATTTTGCTTTCAATTTTAC+AGG - chr1_4:28151917-28151936 None:intergenic 20.0%
!! TCTTATTCATACATATATGA+TGG + chr1_4:28152367-28152386 Msa0158600:CDS 20.0%
!! CTTATTCATACATATATGAT+GGG + chr1_4:28152368-28152387 Msa0158600:CDS 20.0%
!! ATCTTCAAAACAAAATTGAA+AGG + chr1_4:28152988-28153007 Msa0158600:CDS 20.0%
!! TCTTCAAAACAAAATTGAAA+GGG + chr1_4:28152989-28153008 Msa0158600:CDS 20.0%
!!! ATTAATATGGGTAATGTTTT+TGG + chr1_4:28153132-28153151 Msa0158600:intron 20.0%
!!! TTTTTGACTTATTTTAGCTT+AGG + chr1_4:28153551-28153570 Msa0158600:intron 20.0%
!! CTGATTTAAATATGTTCATA+TGG - chr1_4:28153596-28153615 None:intergenic 20.0%
!! TCATGTTAAGTATAAAAACA+GGG - chr1_4:28153665-28153684 None:intergenic 20.0%
!!! TATTTTATTCTTTTTGAACC+AGG + chr1_4:28153725-28153744 Msa0158600:intron 20.0%
!!! AATCAAACTTTTGCTTATAT+TGG + chr1_4:28154265-28154284 Msa0158600:intron 20.0%
!! TGAATGCTATTAAAGAAAAT+AGG - chr1_4:28154710-28154729 None:intergenic 20.0%
!! TAAATGTCAATATATAACCA+AGG - chr1_4:28154785-28154804 None:intergenic 20.0%
!!! TTTCATCATTGTTATTTTTC+AGG + chr1_4:28154807-28154826 Msa0158600:intron 20.0%
!!! TTCATCATTGTTATTTTTCA+GGG + chr1_4:28154808-28154827 Msa0158600:intron 20.0%
!! CTGATTTAAATGTCTAATTA+GGG + chr1_4:28154897-28154916 Msa0158600:three_prime_UTR 20.0%
!!! ATTTATTTGTTGATTAACAC+GGG + chr1_4:28154927-28154946 Msa0158600:three_prime_UTR 20.0%
!!! TAATTATAATCCTTTTTCGA+GGG - chr1_4:28155012-28155031 None:intergenic 20.0%
!!! ATAATTATAATCCTTTTTCG+AGG - chr1_4:28155013-28155032 None:intergenic 20.0%
!! ATGCAAAGTTCAAAATCTTT+TGG + chr1_4:28151737-28151756 Msa0158600:CDS 25.0%
!! TGCAAAGTTCAAAATCTTTT+GGG + chr1_4:28151738-28151757 Msa0158600:CDS 25.0%
! TGTTCGAATCAATGATATTT+CGG + chr1_4:28152639-28152658 Msa0158600:CDS 25.0%
!!! AAGTTTTTTGTTACCATCAT+AGG - chr1_4:28152860-28152879 None:intergenic 25.0%
!!! TTGTTTTGAAGATTTCAAGT+TGG - chr1_4:28152981-28153000 None:intergenic 25.0%
! GTCATGTTAAGTATAAAAAC+AGG - chr1_4:28153666-28153685 None:intergenic 25.0%
! TATACTTAACATGACATCTT+AGG + chr1_4:28153671-28153690 Msa0158600:intron 25.0%
! CCATCTATTATACAACTATA+GGG - chr1_4:28153959-28153978 None:intergenic 25.0%
! TCCATCTATTATACAACTAT+AGG - chr1_4:28153960-28153979 None:intergenic 25.0%
! CTATAATATCAAGAATGTCA+TGG - chr1_4:28154187-28154206 None:intergenic 25.0%
!!! ATTGGACATGTATTTTGAAA+CGG - chr1_4:28154394-28154413 None:intergenic 25.0%
!!! CTGAAGAATTTGGAATTTTA+AGG + chr1_4:28154849-28154868 Msa0158600:CDS 25.0%
!! GCTGATTTAAATGTCTAATT+AGG + chr1_4:28154896-28154915 Msa0158600:three_prime_UTR 25.0%
!!! AATTATAATCCTTTTTCGAG+GGG - chr1_4:28155011-28155030 None:intergenic 25.0%
! TACCTTTAGTATAATCCTAT+TGG + chr1_4:28155066-28155085 Msa0158600:three_prime_UTR 25.0%
! TACCAATAGGATTATACTAA+AGG - chr1_4:28155071-28155090 None:intergenic 25.0%
TTATCTTGTCAGAAGATGAA+AGG - chr1_4:28151796-28151815 None:intergenic 30.0%
! TTGCTTTCAATTTTACAGGT+TGG - chr1_4:28151913-28151932 None:intergenic 30.0%
! TTGGTGACCAATTTCAATAT+AGG + chr1_4:28152029-28152048 Msa0158600:CDS 30.0%
TGGTGACCAATTTCAATATA+GGG + chr1_4:28152030-28152049 Msa0158600:CDS 30.0%
!! TCATGTGGTATCGATTATTT+GGG + chr1_4:28152088-28152107 Msa0158600:CDS 30.0%
!! GTATCGATTATTTGGGTAAA+GGG + chr1_4:28152095-28152114 Msa0158600:CDS 30.0%
! TATCGATTATTTGGGTAAAG+GGG + chr1_4:28152096-28152115 Msa0158600:CDS 30.0%
TTTGTGGAATAGTATGAATG+CGG + chr1_4:28152273-28152292 Msa0158600:CDS 30.0%
! TTCTGAAAAATCTGAAGTGA+TGG + chr1_4:28152324-28152343 Msa0158600:CDS 30.0%
!!! ATATGATGGGTTGTATTTTG+TGG + chr1_4:28152381-28152400 Msa0158600:CDS 30.0%
GTTTGTGCTTAAGAAAATTC+AGG + chr1_4:28152450-28152469 Msa0158600:intron 30.0%
!!! TTGTCTTTTTCGGAAAGTTT+TGG - chr1_4:28152587-28152606 None:intergenic 30.0%
! CTCTAGACATTTGTCTTTTT+CGG - chr1_4:28152597-28152616 None:intergenic 30.0%
! TTGGATAAGTCATATTGACT+AGG - chr1_4:28152738-28152757 None:intergenic 30.0%
!! ATATAACGGATGATTTCAGA+GGG - chr1_4:28152888-28152907 None:intergenic 30.0%
!! TATATAACGGATGATTTCAG+AGG - chr1_4:28152889-28152908 None:intergenic 30.0%
TCAAAACAAAATTGAAAGGG+TGG + chr1_4:28152992-28153011 Msa0158600:CDS 30.0%
CAAAACAAAATTGAAAGGGT+GGG + chr1_4:28152993-28153012 Msa0158600:CDS 30.0%
AAAACAAAATTGAAAGGGTG+GGG + chr1_4:28152994-28153013 Msa0158600:CDS 30.0%
!!! GTTGATTTGATCCAACTTTT+TGG - chr1_4:28153095-28153114 None:intergenic 30.0%
ACATAACATCTTTCACAACT+AGG - chr1_4:28153319-28153338 None:intergenic 30.0%
! TGTAGCAAACAACATTTTGT+GGG - chr1_4:28153366-28153385 None:intergenic 30.0%
! TTGTAGCAAACAACATTTTG+TGG - chr1_4:28153367-28153386 None:intergenic 30.0%
! CAAAATGTTGTTTGCTACAA+AGG + chr1_4:28153367-28153386 Msa0158600:CDS 30.0%
TTATGATTACAATTGCTGCA+AGG + chr1_4:28153415-28153434 Msa0158600:CDS 30.0%
!! TTGAGTAAAATTTGCTACTG+TGG + chr1_4:28153452-28153471 Msa0158600:CDS 30.0%
ATGGACAAATCTACATTTGA+AGG + chr1_4:28153489-28153508 Msa0158600:intron 30.0%
! AGAGTATTATAGTGGGATAA+TGG + chr1_4:28153885-28153904 Msa0158600:intron 30.0%
! GAGTATTATAGTGGGATAAT+GGG + chr1_4:28153886-28153905 Msa0158600:intron 30.0%
CCCTATAGTTGTATAATAGA+TGG + chr1_4:28153956-28153975 Msa0158600:intron 30.0%
CATGAGAAGAGTTTATAAAG+AGG - chr1_4:28154050-28154069 None:intergenic 30.0%
GTAGTCTTTATTATAGTGAC+CGG - chr1_4:28154218-28154237 None:intergenic 30.0%
CTATAAATATATACACCCTC+CGG - chr1_4:28154294-28154313 None:intergenic 30.0%
AAAGTGCACATTGCAAAAAT+TGG - chr1_4:28154412-28154431 None:intergenic 30.0%
!! CTTGGACATGTATTTTGAAA+CGG + chr1_4:28154614-28154633 Msa0158600:intron 30.0%
ATAAAACGTAATTCAAGCCT+TGG + chr1_4:28154765-28154784 Msa0158600:intron 30.0%
!! AATTTTAAGGACCAGCAATT+AGG + chr1_4:28154862-28154881 Msa0158600:exon 30.0%
!! ATTTTAAGGACCAGCAATTA+GGG + chr1_4:28154863-28154882 Msa0158600:exon 30.0%
GATCGAATATGTTTACCAAT+AGG - chr1_4:28155084-28155103 None:intergenic 30.0%
!!! GTAGTGACACTGTTTTGAAA+CGG + chr1_4:28151816-28151835 Msa0158600:CDS 35.0%
CTTCATAAACCTTGTAGCTT+CGG - chr1_4:28151951-28151970 None:intergenic 35.0%
AAAACACCGGAAAGAGAATA+GGG + chr1_4:28151987-28152006 Msa0158600:CDS 35.0%
CATGTAGATGGAATTGAAGT+TGG + chr1_4:28152010-28152029 Msa0158600:CDS 35.0%
GAGACCGATTACAATAAGTT+CGG - chr1_4:28152056-28152075 None:intergenic 35.0%
!! CTCATGTGGTATCGATTATT+TGG + chr1_4:28152087-28152106 Msa0158600:CDS 35.0%
!! GGTATCGATTATTTGGGTAA+AGG + chr1_4:28152094-28152113 Msa0158600:CDS 35.0%
TCTATGAGTTACAACAATGC+TGG - chr1_4:28152137-28152156 None:intergenic 35.0%
TCTGGTTGTTTGCTTTATGA+AGG + chr1_4:28152178-28152197 Msa0158600:intron 35.0%
TGTTTGCTTTATGAAGGTCA+TGG + chr1_4:28152184-28152203 Msa0158600:intron 35.0%
ATGGTGGTAACCCTAATATA+AGG + chr1_4:28152203-28152222 Msa0158600:CDS 35.0%
TGGTGGTAACCCTAATATAA+GGG + chr1_4:28152204-28152223 Msa0158600:CDS 35.0%
TTGTGGAATAGTATGAATGC+GGG + chr1_4:28152274-28152293 Msa0158600:CDS 35.0%
TAAGAAAATTCAGGACCAAC+CGG + chr1_4:28152459-28152478 Msa0158600:intron 35.0%
! AAACATTCTTTGGGAGAACA+TGG - chr1_4:28152543-28152562 None:intergenic 35.0%
CTCGAGGAAAAACATTCTTT+GGG - chr1_4:28152552-28152571 None:intergenic 35.0%
TCTCGAGGAAAAACATTCTT+TGG - chr1_4:28152553-28152572 None:intergenic 35.0%
! TGTTTTTCCTCGAGATGTTA+TGG + chr1_4:28152558-28152577 Msa0158600:CDS 35.0%
CGAAAAAGACAAATGTCTAG+AGG + chr1_4:28152595-28152614 Msa0158600:CDS 35.0%
!! ATTGATTCGAACAACATCCT+TGG - chr1_4:28152632-28152651 None:intergenic 35.0%
TCGAATCAATGATATTTCGG+AGG + chr1_4:28152642-28152661 Msa0158600:CDS 35.0%
CGAATCAATGATATTTCGGA+GGG + chr1_4:28152643-28152662 Msa0158600:CDS 35.0%
GCAAAGAAAAGTTCCCTATT+CGG + chr1_4:28152665-28152684 Msa0158600:CDS 35.0%
CAAAGAAAAGTTCCCTATTC+GGG + chr1_4:28152666-28152685 Msa0158600:CDS 35.0%
! ATATTGACTAGGTAACCGAA+GGG - chr1_4:28152727-28152746 None:intergenic 35.0%
! AGTATTGAACGATTCGTCTT+TGG - chr1_4:28152757-28152776 None:intergenic 35.0%
AAGACGAATCGTTCAATACT+AGG + chr1_4:28152757-28152776 Msa0158600:CDS 35.0%
TGTGTTTGCATAGCTAAGAA+TGG + chr1_4:28152823-28152842 Msa0158600:CDS 35.0%
TTTGCATAGCTAAGAATGGT+GGG + chr1_4:28152827-28152846 Msa0158600:CDS 35.0%
!! TATAACGGATGATTTCAGAG+GGG - chr1_4:28152887-28152906 None:intergenic 35.0%
TCATCCGTTATATATGAGTG+TGG + chr1_4:28152895-28152914 Msa0158600:CDS 35.0%
ATGAAGAACACAAGCAAGAA+GGG - chr1_4:28152921-28152940 None:intergenic 35.0%
CATCACAACAACCAAAAAGT+TGG + chr1_4:28153081-28153100 Msa0158600:CDS 35.0%
! TGATGTTGTATGCATTTTGC+AGG + chr1_4:28153215-28153234 Msa0158600:intron 35.0%
TTCATTGATGCAACTAGACA+TGG + chr1_4:28153254-28153273 Msa0158600:CDS 35.0%
ACATCTTTCACAACTAGGTT+AGG - chr1_4:28153314-28153333 None:intergenic 35.0%
! GTAGCAAACAACATTTTGTG+GGG - chr1_4:28153365-28153384 None:intergenic 35.0%
!! AATTTGCTACTGTGGTTCAT+TGG + chr1_4:28153460-28153479 Msa0158600:intron 35.0%
! CTTCTAAAAACCCAAGAAGA+GGG - chr1_4:28153770-28153789 None:intergenic 35.0%
GATGAAAATGGGTACCTATT+GGG + chr1_4:28153861-28153880 Msa0158600:intron 35.0%
ATGAAAATGGGTACCTATTG+GGG + chr1_4:28153862-28153881 Msa0158600:intron 35.0%
ACTATAATACTCTCCCCAAT+AGG - chr1_4:28153878-28153897 None:intergenic 35.0%
!! TATTGGGGAGAGTATTATAG+TGG + chr1_4:28153877-28153896 Msa0158600:intron 35.0%
!! ATTGGGGAGAGTATTATAGT+GGG + chr1_4:28153878-28153897 Msa0158600:intron 35.0%
CTAATAATACTCTCCCCAAT+GGG - chr1_4:28153914-28153933 None:intergenic 35.0%
ACTAATAATACTCTCCCCAA+TGG - chr1_4:28153915-28153934 None:intergenic 35.0%
!! ATTGGGGAGAGTATTATTAG+TGG + chr1_4:28153914-28153933 Msa0158600:intron 35.0%
!! TTGGGGAGAGTATTATTAGT+GGG + chr1_4:28153915-28153934 Msa0158600:intron 35.0%
ATTAGTGGGATGACAATTAG+TGG + chr1_4:28153929-28153948 Msa0158600:intron 35.0%
TGATATTATAGTACACCCTC+CGG + chr1_4:28154196-28154215 Msa0158600:intron 35.0%
TCTTTATTATAGTGACCGGA+GGG - chr1_4:28154214-28154233 None:intergenic 35.0%
!! CTTTTGCTTATATTGGTGAC+CGG + chr1_4:28154272-28154291 Msa0158600:intron 35.0%
!!! GACATGTATTTTGAAACGGA+TGG - chr1_4:28154618-28154637 None:intergenic 35.0%
TAACGGTCAAAATTGTGCAT+TGG + chr1_4:28154573-28154592 Msa0158600:intron 35.0%
!!! GACATGTATTTTGAAACGGA+GGG + chr1_4:28154618-28154637 Msa0158600:intron 35.0%
!!! TATTTTTCAGGGCAGTATGT+TGG + chr1_4:28154819-28154838 Msa0158600:intron 35.0%
!!! ATTTTTCAGGGCAGTATGTT+GGG + chr1_4:28154820-28154839 Msa0158600:intron 35.0%
ATTATGTACACCCTAATTGC+TGG - chr1_4:28154876-28154895 None:intergenic 35.0%
TAATTAGTACCCCTCGAAAA+AGG + chr1_4:28154999-28155018 Msa0158600:three_prime_UTR 35.0%
AAACATATTCGATCAATGCC+TGG + chr1_4:28155090-28155109 Msa0158600:three_prime_UTR 35.0%
!!! CACTGTTTTGAAACGGAGAA+AGG + chr1_4:28151823-28151842 Msa0158600:CDS 40.0%
CGAAGCTACAAGGTTTATGA+AGG + chr1_4:28151949-28151968 Msa0158600:intron 40.0%
TTATGAAGGCGAAACGTCAA+TGG + chr1_4:28151963-28151982 Msa0158600:CDS 40.0%
TATGAAGGCGAAACGTCAAT+GGG + chr1_4:28151964-28151983 Msa0158600:CDS 40.0%
AAACGTCAATGGGAAAACAC+CGG + chr1_4:28151974-28151993 Msa0158600:CDS 40.0%
GAAAACACCGGAAAGAGAAT+AGG + chr1_4:28151986-28152005 Msa0158600:CDS 40.0%
TACATGCCCTATTCTCTTTC+CGG - chr1_4:28151996-28152015 None:intergenic 40.0%
AAGAGAATAGGGCATGTAGA+TGG + chr1_4:28151998-28152017 Msa0158600:CDS 40.0%
GTTCGGCCCTATATTGAAAT+TGG - chr1_4:28152039-28152058 None:intergenic 40.0%
AGGGCCGAACTTATTGTAAT+CGG + chr1_4:28152049-28152068 Msa0158600:CDS 40.0%
! TACCACATGAGTGTTGTTGA+TGG - chr1_4:28152078-28152097 None:intergenic 40.0%
GATTATTTGGGTAAAGGGGA+GGG + chr1_4:28152100-28152119 Msa0158600:CDS 40.0%
GCGAATGTTATGAAGTCTTC+TGG + chr1_4:28152160-28152179 Msa0158600:CDS 40.0%
TTGCTTTATGAAGGTCATGG+TGG + chr1_4:28152187-28152206 Msa0158600:intron 40.0%
GGTAACCCTAATATAAGGGA+CGG + chr1_4:28152208-28152227 Msa0158600:CDS 40.0%
TGTGGAATAGTATGAATGCG+GGG + chr1_4:28152275-28152294 Msa0158600:CDS 40.0%
TCCTGTTAGGGTTATATTGC+AGG + chr1_4:28152300-28152319 Msa0158600:CDS 40.0%
ACCTGCAATATAACCCTAAC+AGG - chr1_4:28152304-28152323 None:intergenic 40.0%
ACTCGAGTAAGAGGACAATT+TGG + chr1_4:28152412-28152431 Msa0158600:CDS 40.0%
AAGTTCCCATAACATCTCGA+GGG - chr1_4:28152495-28152514 None:intergenic 40.0%
AAAGTTCCCATAACATCTCG+AGG - chr1_4:28152496-28152515 None:intergenic 40.0%
CTTCAGAATTGTGTCTCTTC+TGG - chr1_4:28152519-28152538 None:intergenic 40.0%
CAGAAGAGACACAATTCTGA+AGG + chr1_4:28152517-28152536 Msa0158600:CDS 40.0%
TTGGTTTCCATAACATCTCG+AGG - chr1_4:28152568-28152587 None:intergenic 40.0%
GGTATCTCCACATAGCTTAT+AGG - chr1_4:28152700-28152719 None:intergenic 40.0%
! CATATTGACTAGGTAACCGA+AGG - chr1_4:28152728-28152747 None:intergenic 40.0%
ACGAATCGTTCAATACTAGG+TGG + chr1_4:28152760-28152779 Msa0158600:CDS 40.0%
AATCGTTCAATACTAGGTGG+TGG + chr1_4:28152763-28152782 Msa0158600:CDS 40.0%
GTTTGCATAGCTAAGAATGG+TGG + chr1_4:28152826-28152845 Msa0158600:CDS 40.0%
TTGCATAGCTAAGAATGGTG+GGG + chr1_4:28152828-28152847 Msa0158600:CDS 40.0%
AGGGCCACACTCATATATAA+CGG - chr1_4:28152902-28152921 None:intergenic 40.0%
GATGAAGAACACAAGCAAGA+AGG - chr1_4:28152922-28152941 None:intergenic 40.0%
! GAATACCAGATTGACTCACT+CGG - chr1_4:28152954-28152973 None:intergenic 40.0%
GCAACTAGACATGGAAACAT+TGG + chr1_4:28153263-28153282 Msa0158600:CDS 40.0%
CTAGACATGGAAACATTGGA+AGG + chr1_4:28153267-28153286 Msa0158600:CDS 40.0%
TTGCTGCAAGGTGAATATCA+AGG + chr1_4:28153427-28153446 Msa0158600:CDS 40.0%
TGTGGTTCATTGGAATGCAA+TGG + chr1_4:28153470-28153489 Msa0158600:intron 40.0%
AAAACAGGGTTGAGTCTCAT+TGG - chr1_4:28153651-28153670 None:intergenic 40.0%
AAAAACCCAAGAAGAGGGTT+GGG - chr1_4:28153765-28153784 None:intergenic 40.0%
TAAAAACCCAAGAAGAGGGT+TGG - chr1_4:28153766-28153785 None:intergenic 40.0%
! GCTTCTAAAAACCCAAGAAG+AGG - chr1_4:28153771-28153790 None:intergenic 40.0%
! TGTTGTGATTTCGAGTCAAG+CGG + chr1_4:28153809-28153828 Msa0158600:intron 40.0%
GGATGAAAATGGGTACCTAT+TGG + chr1_4:28153860-28153879 Msa0158600:intron 40.0%
AGTGGGATTTAGTCTCACAT+CGG - chr1_4:28154085-28154104 None:intergenic 40.0%
GACTAAATCCCACTGAGTAT+TGG + chr1_4:28154091-28154110 Msa0158600:intron 40.0%
GTCTTTATTATAGTGACCGG+AGG - chr1_4:28154215-28154234 None:intergenic 40.0%
CATATGTGAAGTGGTTGACT+TGG + chr1_4:28154596-28154615 Msa0158600:intron 40.0%
!!! GGACATGTATTTTGAAACGG+AGG + chr1_4:28154617-28154636 Msa0158600:intron 40.0%
! ATTTGTTGATTAACACGGGC+TGG + chr1_4:28154931-28154950 Msa0158600:three_prime_UTR 40.0%
! TTTGTTGATTAACACGGGCT+GGG + chr1_4:28154932-28154951 Msa0158600:three_prime_UTR 40.0%
! TTGGGAGAAAGCGTGAAGAA+TGG + chr1_4:28151756-28151775 Msa0158600:CDS 45.0%
CTCCATCAACAACACTCATG+TGG + chr1_4:28152073-28152092 Msa0158600:CDS 45.0%
CGATTATTTGGGTAAAGGGG+AGG + chr1_4:28152099-28152118 Msa0158600:CDS 45.0%
CGACACCGTCCCTTATATTA+GGG - chr1_4:28152216-28152235 None:intergenic 45.0%
GACAATGTGACTCGAGTAAG+AGG + chr1_4:28152403-28152422 Msa0158600:CDS 45.0%
TGTTTCCCCTCGAGATGTTA+TGG + chr1_4:28152486-28152505 Msa0158600:CDS 45.0%
GTTTCCCCTCGAGATGTTAT+GGG + chr1_4:28152487-28152506 Msa0158600:CDS 45.0%
AGTTCCCATAACATCTCGAG+GGG - chr1_4:28152494-28152513 None:intergenic 45.0%
!! TAGAGGTCTTGTTCTCTCCA+AGG + chr1_4:28152612-28152631 Msa0158600:CDS 45.0%
AGAAAAGTTCCCTATTCGGG+TGG + chr1_4:28152669-28152688 Msa0158600:CDS 45.0%
GGTGACACCTATAAGCTATG+TGG + chr1_4:28152690-28152709 Msa0158600:intron 45.0%
GTGGAGATACCTAAACCCTT+CGG + chr1_4:28152709-28152728 Msa0158600:intron 45.0%
ACTAGGTAACCGAAGGGTTT+AGG - chr1_4:28152721-28152740 None:intergenic 45.0%
ATCAACCGAGTGAGTCAATC+TGG + chr1_4:28152946-28152965 Msa0158600:intron 45.0%
ACACGCTCTTTCATTCCATC+CGG + chr1_4:28153024-28153043 Msa0158600:CDS 45.0%
! AGCTTTGTGTGTGAGCTCAT+TGG + chr1_4:28153048-28153067 Msa0158600:CDS 45.0%
! ATGCATTTTGCAGGTGCTTC+TGG + chr1_4:28153224-28153243 Msa0158600:intron 45.0%
ATAACTCAGCTCTCTACCTG+CGG - chr1_4:28153399-28153418 None:intergenic 45.0%
AAAACCCAAGAAGAGGGTTG+GGG - chr1_4:28153764-28153783 None:intergenic 45.0%
! CCATTTTCATCCCACTCTTG+CGG - chr1_4:28153852-28153871 None:intergenic 45.0%
CGCAAGAGTGGGATGAAAAT+GGG + chr1_4:28153850-28153869 Msa0158600:intron 45.0%
!! TGGGATAATGGGTACCCATT+GGG + chr1_4:28153897-28153916 Msa0158600:intron 45.0%
GTTAGTGGCCAATACTCAGT+GGG - chr1_4:28154102-28154121 None:intergenic 45.0%
TGTTAGTGGCCAATACTCAG+TGG - chr1_4:28154103-28154122 None:intergenic 45.0%
TCATGGTATCAGAGCCTATC+GGG - chr1_4:28154170-28154189 None:intergenic 45.0%
GTCATGGTATCAGAGCCTAT+CGG - chr1_4:28154171-28154190 None:intergenic 45.0%
! TTGCTTATATTGGTGACCGG+AGG + chr1_4:28154275-28154294 Msa0158600:intron 45.0%
! TGCTTATATTGGTGACCGGA+GGG + chr1_4:28154276-28154295 Msa0158600:intron 45.0%
! GTGCATTGGCATATGTGAAG+TGG + chr1_4:28154587-28154606 Msa0158600:intron 45.0%
TGGGCGCTTACTGAAGAATT+TGG + chr1_4:28154839-28154858 Msa0158600:CDS 45.0%
! TTGTTGATTAACACGGGCTG+GGG + chr1_4:28154933-28154952 Msa0158600:three_prime_UTR 45.0%
!! CTGGGGTTTTAATGTTGGCA+GGG + chr1_4:28154950-28154969 Msa0158600:three_prime_UTR 45.0%
GCGACACCGTCCCTTATATT+AGG - chr1_4:28152217-28152236 None:intergenic 50.0%
CGCGCCTCATGATCAGAAAT+TGG + chr1_4:28152234-28152253 Msa0158600:CDS 50.0%
CGCGCCAATTTCTGATCATG+AGG - chr1_4:28152241-28152260 None:intergenic 50.0%
CATGATCAGAAATTGGCGCG+CGG + chr1_4:28152241-28152260 Msa0158600:CDS 50.0%
! TAGGTGTCACCACCCGAATA+GGG - chr1_4:28152681-28152700 None:intergenic 50.0%
! ATAGGTGTCACCACCCGAAT+AGG - chr1_4:28152682-28152701 None:intergenic 50.0%
TCACACACAAAGCTTCCGGA+TGG - chr1_4:28153042-28153061 None:intergenic 50.0%
GAGCTCACACACAAAGCTTC+CGG - chr1_4:28153046-28153065 None:intergenic 50.0%
TAACTCAGCTCTCTACCTGC+GGG - chr1_4:28153398-28153417 None:intergenic 50.0%
TGGGGTTTGAGTAGAAGTCC+TGG - chr1_4:28153746-28153765 None:intergenic 50.0%
TCAAACCCCAACCCTCTTCT+TGG + chr1_4:28153756-28153775 Msa0158600:intron 50.0%
CAAACCCCAACCCTCTTCTT+GGG + chr1_4:28153757-28153776 Msa0158600:intron 50.0%
CGGAAACATACCGCAAGAGT+GGG + chr1_4:28153839-28153858 Msa0158600:intron 50.0%
CCGCAAGAGTGGGATGAAAA+TGG + chr1_4:28153849-28153868 Msa0158600:intron 50.0%
!! GTGGGATAATGGGTACCCAT+TGG + chr1_4:28153896-28153915 Msa0158600:intron 50.0%
!! GGGATAATGGGTACCCATTG+GGG + chr1_4:28153898-28153917 Msa0158600:intron 50.0%
!!! ACGGGCTGGGGTTTTAATGT+TGG + chr1_4:28154945-28154964 Msa0158600:three_prime_UTR 50.0%
!! GCTGGGGTTTTAATGTTGGC+AGG + chr1_4:28154949-28154968 Msa0158600:three_prime_UTR 50.0%
! GGGCTTTAGCCGAAGCTACA+AGG + chr1_4:28151939-28151958 Msa0158600:intron 55.0%
GGGTAAAGGGGAGGGTTCTT+TGG + chr1_4:28152108-28152127 Msa0158600:CDS 55.0%
CGCGCGGAAATCTTGCTTTG+TGG + chr1_4:28152257-28152276 Msa0158600:CDS 55.0%
TGAATGCGGGGAGTCCTGTT+AGG + chr1_4:28152287-28152306 Msa0158600:CDS 55.0%
GAATGCGGGGAGTCCTGTTA+GGG + chr1_4:28152288-28152307 Msa0158600:CDS 55.0%
TCTCGAGGGGAAACACTAGC+CGG - chr1_4:28152481-28152500 None:intergenic 55.0%
! GGTGGGGCATTAGCCTATGA+TGG + chr1_4:28152844-28152863 Msa0158600:CDS 55.0%
GCTACAAAGGACATACCCGC+AGG + chr1_4:28153380-28153399 Msa0158600:CDS 55.0%
TCGAGTCAAGCGGAGTCAAG+CGG + chr1_4:28153819-28153838 Msa0158600:intron 55.0%
GCGGAAACATACCGCAAGAG+TGG + chr1_4:28153838-28153857 Msa0158600:intron 55.0%
ATCGTTGGGCTTGCATCGTC+GGG - chr1_4:28154144-28154163 None:intergenic 55.0%
CATCGTTGGGCTTGCATCGT+CGG - chr1_4:28154145-28154164 None:intergenic 55.0%
AGCCTATCGGGTCCATCGTT+GGG - chr1_4:28154158-28154177 None:intergenic 55.0%
GAGGGGAAACACTAGCCGGT+TGG - chr1_4:28152477-28152496 None:intergenic 60.0%
! AGCGTGAGGGGGTGTGTTAG+TGG - chr1_4:28154117-28154136 None:intergenic 60.0%
ATCGTCGGGCTGAAGCGTGA+GGG - chr1_4:28154130-28154149 None:intergenic 60.0%
CGACGATGCAAGCCCAACGA+TGG + chr1_4:28154143-28154162 Msa0158600:intron 60.0%
AGCCCAACGATGGACCCGAT+AGG + chr1_4:28154153-28154172 Msa0158600:intron 60.0%
GAGCCTATCGGGTCCATCGT+TGG - chr1_4:28154159-28154178 None:intergenic 60.0%
TCGTCGGGCTGAAGCGTGAG+GGG - chr1_4:28154129-28154148 None:intergenic 65.0%
CATCGTCGGGCTGAAGCGTG+AGG - chr1_4:28154131-28154150 None:intergenic 65.0%
CGTCGGGCTGAAGCGTGAGG+GGG - chr1_4:28154128-28154147 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 28151716 28155113 28151716 ID=Msa0158600;Name=Msa0158600
chr1_4 mRNA 28151716 28155113 28151716 ID=Msa0158600-mRNA-1;Parent=Msa0158600;Name=Msa0158600-mRNA-1;_AED=0.05;_eAED=0.09;_QI=0|0|0.12|1|0.14|0|8|244|517
chr1_4 exon 28151716 28151903 28151716 ID=Msa0158600-mRNA-1:exon:9089;Parent=Msa0158600-mRNA-1
chr1_4 exon 28151961 28152181 28151961 ID=Msa0158600-mRNA-1:exon:9090;Parent=Msa0158600-mRNA-1
chr1_4 exon 28152200 28152441 28152200 ID=Msa0158600-mRNA-1:exon:9091;Parent=Msa0158600-mRNA-1
chr1_4 exon 28152472 28152690 28152472 ID=Msa0158600-mRNA-1:exon:9092;Parent=Msa0158600-mRNA-1
chr1_4 exon 28152715 28152954 28152715 ID=Msa0158600-mRNA-1:exon:9093;Parent=Msa0158600-mRNA-1
chr1_4 exon 28152976 28153141 28152976 ID=Msa0158600-mRNA-1:exon:9094;Parent=Msa0158600-mRNA-1
chr1_4 exon 28153237 28153473 28153237 ID=Msa0158600-mRNA-1:exon:9095;Parent=Msa0158600-mRNA-1
chr1_4 exon 28154829 28155113 28154829 ID=Msa0158600-mRNA-1:exon:9096;Parent=Msa0158600-mRNA-1
chr1_4 CDS 28151716 28151903 28151716 ID=Msa0158600-mRNA-1:cds;Parent=Msa0158600-mRNA-1
chr1_4 CDS 28151961 28152181 28151961 ID=Msa0158600-mRNA-1:cds;Parent=Msa0158600-mRNA-1
chr1_4 CDS 28152200 28152441 28152200 ID=Msa0158600-mRNA-1:cds;Parent=Msa0158600-mRNA-1
chr1_4 CDS 28152472 28152690 28152472 ID=Msa0158600-mRNA-1:cds;Parent=Msa0158600-mRNA-1
chr1_4 CDS 28152715 28152954 28152715 ID=Msa0158600-mRNA-1:cds;Parent=Msa0158600-mRNA-1
chr1_4 CDS 28152976 28153141 28152976 ID=Msa0158600-mRNA-1:cds;Parent=Msa0158600-mRNA-1
chr1_4 CDS 28153237 28153473 28153237 ID=Msa0158600-mRNA-1:cds;Parent=Msa0158600-mRNA-1
chr1_4 CDS 28154829 28154869 28154829 ID=Msa0158600-mRNA-1:cds;Parent=Msa0158600-mRNA-1
chr1_4 three_prime_UTR 28154870 28155113 28154870 ID=Msa0158600-mRNA-1:three_prime_utr;Parent=Msa0158600-mRNA-1
Gene Sequence

>Msa0158600

GTGTTTTATTTGCTGATGAAGATGCAAAGTTCAAAATCTTTTGGGAGAAAGCGTGAAGAATGGTCATATACTGAGAACCTTTCATCTTCTGACAAGATAAGTAGTGACACTGTTTTGAAACGGAGAAAGGTGAATAAAGCATTAACAATTTTTAGGGACTTATCACACAAACTCTATTGTGAATACAAGTTTATGAAGGCGAAACGTCAATGGGAAAACACCGGAAAGAGAATAGGGCATGTAGATGGAATTGAAGTTGGTGACCAATTTCAATATAGGGCCGAACTTATTGTAATCGGTCTCCATCAACAACACTCATGTGGTATCGATTATTTGGGTAAAGGGGAGGGTTCTTTGGCAACCAGCATTGTTGTAACTCATAGATATGCGAATGTTATGAAGTCTTCTGGTCATGGTGGTAACCCTAATATAAGGGACGGTGTCGCGCCTCATGATCAGAAATTGGCGCGCGGAAATCTTGCTTTGTGGAATAGTATGAATGCGGGGAGTCCTGTTAGGGTTATATTGCAGGTTTCTGAAAAATCTGAAGTGATGGAAGAGTTGAGCAACAATGTTTCTTATTCATACATATATGATGGGTTGTATTTTGTGGACAATGTGACTCGAGTAAGAGGACAATTTGGAAAACTTGACCAACCGGCTAGTGTTTCCCCTCGAGATGTTATGGGAACTTTCCAGAAGAGACACAATTCTGAAGGCCATGTTCTCCCAAAGAATGTTTTTCCTCGAGATGTTATGGAAACCAAAACTTTCCGAAAAAGACAAATGTCTAGAGGTCTTGTTCTCTCCAAGGATGTTGTTCGAATCAATGATATTTCGGAGGGCAAAGAAAAGTTCCCTATTCGGGTGATACCTAAACCCTTCGGTTACCTAGTCAATATGACTTATCCAAAGACGAATCGTTCAATACTAGGTGGTGGATGTAATTGTGAGAATGCATGTGTTGATTGTCTTGAATGTGTTTGCATAGCTAAGAATGGTGGGGCATTAGCCTATGATGGTAACAAAAAACTTGTTTCCCCTCTGAAATCATCCGTTATATATGAGTGTGGCCCTTCTTGCTTGTGTTCTTCATCTTGCATCAACCGATTCCAACTTGAAATCTTCAAAACAAAATTGAAAGGGTGGGGTGTTCGAACACGCTCTTTCATTCCATCCGGAAGCTTTGTGTGTGAGCTCATTGGAGAAGTTATTCATCACAACAACCAAAAAGTTGGATCAAATCAACATGTTGATGACTATATAATTAATATGGGTGCTTCTGGCAAGATCTTCATTGATGCAACTAGACATGGAAACATTGGAAGGTTTATCAATCATAGTTGTTCTCCTAACCTAGTTGTGAAAGATGTTATGTATGATCACAGTGATAAGAGTTTACCCCACAAAATGTTGTTTGCTACAAAGGACATACCCGCAGGTAGAGAGCTGAGTTATGATTACAATTGCTGCAAGGTGAATATCAAGGTTTTGAGTAAAATTTGCTACTGTGGGCAGTATGTTGGGCGCTTACTGAAGAATTTGGAATTTTAA
Protein sequence

>Msa0158600

VFYLLMKMQSSKSFGRKREEWSYTENLSSSDKISSDTVLKRRKVNKALTIFRDLSHKLYCEYKFMKAKRQWENTGKRIGHVDGIEVGDQFQYRAELIVIGLHQQHSCGIDYLGKGEGSLATSIVVTHRYANVMKSSGHGGNPNIRDGVAPHDQKLARGNLALWNSMNAGSPVRVILQVSEKSEVMEELSNNVSYSYIYDGLYFVDNVTRVRGQFGKLDQPASVSPRDVMGTFQKRHNSEGHVLPKNVFPRDVMETKTFRKRQMSRGLVLSKDVVRINDISEGKEKFPIRVIPKPFGYLVNMTYPKTNRSILGGGCNCENACVDCLECVCIAKNGGALAYDGNKKLVSPLKSSVIYECGPSCLCSSSCINRFQLEIFKTKLKGWGVRTRSFIPSGSFVCELIGEVIHHNNQKVGSNQHVDDYIINMGASGKIFIDATRHGNIGRFINHSCSPNLVVKDVMYDHSDKSLPHKMLFATKDIPAGRELSYDYNCCKVNIKVLSKICYCGQYVGRLLKNLEF*