AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0000190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0000190 MtrunA17_Chr7g0241821 98.261 115 2 0 1 115 1 115 1.40e-75 219
Msa0000190 MtrunA17_Chr2g0282121 54.098 122 43 3 4 114 4 123 2.15e-23 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACAATCTTGATTGCTCTT+TGG 0.335761 1_1:-1025489 None:intergenic
TCTTACATTCTTCTTCATCT+AGG 0.348003 1_1:+1025690 Msa0000190:CDS
GTTGGTATGTCATTGACATT+AGG 0.356920 1_1:-1025419 None:intergenic
GGTTTCTTCTTCATCTACTT+TGG 0.392407 1_1:+1025598 Msa0000190:CDS
AAATGTCCATTAAAAGCAAA+AGG 0.418577 1_1:+1025384 None:intergenic
CTTACATTCTTCTTCATCTA+GGG 0.436392 1_1:+1025691 Msa0000190:CDS
CTTCTTCATCTACTTTGGTA+AGG 0.453056 1_1:+1025603 Msa0000190:CDS
TTGAAATTCCTAGTGCAACT+AGG 0.484774 1_1:+1025518 Msa0000190:CDS
AACTCAACTCAACTGAAACT+TGG 0.493461 1_1:-1025719 None:intergenic
AGTTTCAGCTCGATGTGAAT+CGG 0.520615 1_1:-1025648 None:intergenic
AATGTCCATTAAAAGCAAAA+GGG 0.546646 1_1:+1025385 None:intergenic
CTATGAAAAGAAGATCCTCT+AGG 0.575974 1_1:+1025573 Msa0000190:CDS
TACTTTGGTAAGGTCGCGAT+CGG 0.577817 1_1:+1025613 Msa0000190:CDS
AGTTGCACTAGGAATTTCAA+AGG 0.597401 1_1:-1025515 None:intergenic
AAGAAGAGGGAAATTGAAAG+TGG 0.598757 1_1:+1025452 Msa0000190:CDS
GAAAAGAAGATCCTCTAGGA+AGG 0.606833 1_1:+1025577 Msa0000190:CDS
AGTGCAACTAGGAACAAAAG+TGG 0.620566 1_1:+1025529 Msa0000190:CDS
CAGCTCGATGTGAATCGGTG+AGG 0.671793 1_1:-1025643 None:intergenic
TGAAGAAGAAACCTTCCTAG+AGG 0.674397 1_1:-1025588 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTTTTTCTCAAGAAGA+GGG + chr1_1:1025439-1025458 Msa0000190:CDS 25.0%
CTCTTCTTGAGAAAAAATGT+TGG - chr1_1:1025440-1025459 None:intergenic 30.0%
!!! CAACATTTTTTCTCAAGAAG+AGG + chr1_1:1025438-1025457 Msa0000190:CDS 30.0%
TCTTACATTCTTCTTCATCT+AGG + chr1_1:1025690-1025709 Msa0000190:CDS 30.0%
CTTACATTCTTCTTCATCTA+GGG + chr1_1:1025691-1025710 Msa0000190:CDS 30.0%
!! GCATACCCTTTTGCTTTTAA+TGG - chr1_1:1025393-1025412 None:intergenic 35.0%
GTTGGTATGTCATTGACATT+AGG - chr1_1:1025422-1025441 None:intergenic 35.0%
AAGAAGAGGGAAATTGAAAG+TGG + chr1_1:1025452-1025471 Msa0000190:CDS 35.0%
TGACAATCTTGATTGCTCTT+TGG - chr1_1:1025492-1025511 None:intergenic 35.0%
AGTTGCACTAGGAATTTCAA+AGG - chr1_1:1025518-1025537 None:intergenic 35.0%
TTGAAATTCCTAGTGCAACT+AGG + chr1_1:1025518-1025537 Msa0000190:CDS 35.0%
CTATGAAAAGAAGATCCTCT+AGG + chr1_1:1025573-1025592 Msa0000190:CDS 35.0%
GGTTTCTTCTTCATCTACTT+TGG + chr1_1:1025598-1025617 Msa0000190:CDS 35.0%
CTTCTTCATCTACTTTGGTA+AGG + chr1_1:1025603-1025622 Msa0000190:CDS 35.0%
! CTTTTGTTCCTAGTTGCACT+AGG - chr1_1:1025529-1025548 None:intergenic 40.0%
AGTGCAACTAGGAACAAAAG+TGG + chr1_1:1025529-1025548 Msa0000190:CDS 40.0%
! GAAAAGAAGATCCTCTAGGA+AGG + chr1_1:1025577-1025596 Msa0000190:CDS 40.0%
TGAAGAAGAAACCTTCCTAG+AGG - chr1_1:1025591-1025610 None:intergenic 40.0%
AGTTTCAGCTCGATGTGAAT+CGG - chr1_1:1025651-1025670 None:intergenic 40.0%
TACTTTGGTAAGGTCGCGAT+CGG + chr1_1:1025613-1025632 Msa0000190:CDS 45.0%
CAGCTCGATGTGAATCGGTG+AGG - chr1_1:1025646-1025665 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 1025386 1025733 1025386 ID=Msa0000190;Name=Msa0000190
chr1_1 mRNA 1025386 1025733 1025386 ID=Msa0000190-mRNA-1;Parent=Msa0000190;Name=Msa0000190-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|115
chr1_1 exon 1025386 1025733 1025386 ID=Msa0000190-mRNA-1:exon:101;Parent=Msa0000190-mRNA-1
chr1_1 CDS 1025386 1025733 1025386 ID=Msa0000190-mRNA-1:cds;Parent=Msa0000190-mRNA-1
Gene Sequence

>Msa0000190

ATGTCCATTAAAAGCAAAAGGGTATGCTTTAGTCCTAATGTCAATGACATACCAACATTTTTTCTCAAGAAGAGGGAAATTGAAAGTGGTAATTTCAAATTAACCAAAGAGCAATCAAGATTGTCATCACCTTTGAAATTCCTAGTGCAACTAGGAACAAAAGTGGCAAGTTCTATAAGAAGTGTTTCTATGAAAAGAAGATCCTCTAGGAAGGTTTCTTCTTCATCTACTTTGGTAAGGTCGCGATCGGTATCAGACCTCACCGATTCACATCGAGCTGAAACTGTTGAAGATTGCATTGAGTTCTTACATTCTTCTTCATCTAGGGAGAGACCAAGTTTCAGTTGA

Protein sequence

>Msa0000190

MSIKSKRVCFSPNVNDIPTFFLKKREIESGNFKLTKEQSRLSSPLKFLVQLGTKVASSIRSVSMKRRSSRKVSSSSTLVRSRSVSDLTDSHRAETVEDCIEFLHSSSSRERPSFS*