AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0783850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0783850 MtrunA17_Chr5g0437681 98.249 571 6 1 1 571 1 567 0.0 1161
Msa0783850 MtrunA17_Chr3g0114211 80.769 572 95 4 4 571 10 570 0.0 964
Msa0783850 MtrunA17_Chr5g0431581 58.916 572 222 5 1 571 1 560 0.0 720
Msa0783850 MtrunA17_Chr5g0404361 49.912 569 261 6 7 571 63 611 0.0 597
Msa0783850 MtrunA17_Chr5g0404341 50.613 571 256 8 7 571 16 566 0.0 589
Msa0783850 MtrunA17_Chr7g0238941 51.658 573 254 10 9 571 19 578 0.0 584
Msa0783850 MtrunA17_Chr7g0237491 50.862 580 263 12 4 571 11 580 0.0 578
Msa0783850 MtrunA17_Chr7g0238981 49.574 587 264 10 7 571 17 593 0.0 577
Msa0783850 MtrunA17_Chr4g0031901 51.601 562 249 11 4 555 21 569 0.0 573
Msa0783850 MtrunA17_Chr5g0429511 48.944 568 266 8 8 571 11 558 0.0 573
Msa0783850 MtrunA17_Chr4g0005921 49.217 575 270 7 1 571 1 557 0.0 571
Msa0783850 MtrunA17_Chr7g0237441 49.568 579 268 11 6 571 13 580 0.0 569
Msa0783850 MtrunA17_Chr2g0278811 46.633 594 273 7 11 571 25 607 0.0 566
Msa0783850 MtrunA17_Chr7g0238351 49.735 567 263 12 16 571 26 581 0.0 563
Msa0783850 MtrunA17_Chr5g0436461 49.387 571 261 8 8 571 5 554 0.0 557
Msa0783850 MtrunA17_Chr3g0104061 48.258 574 272 9 2 571 4 556 0.0 553
Msa0783850 MtrunA17_Chr7g0240971 48.885 583 273 10 1 571 1 570 0.0 541
Msa0783850 MtrunA17_Chr4g0008521 48.750 560 260 9 25 571 27 572 0.0 529
Msa0783850 MtrunA17_Chr7g0240991 45.581 577 284 11 10 571 10 571 0.0 528
Msa0783850 MtrunA17_Chr8g0346581 44.482 589 291 7 1 571 1 571 3.34e-177 513
Msa0783850 MtrunA17_Chr3g0133861 46.707 334 164 6 230 557 1 326 2.45e-101 310
Msa0783850 MtrunA17_Chr8g0382691 31.356 590 317 21 19 571 29 567 2.62e-70 236
Msa0783850 MtrunA17_Chr3g0133871 49.315 219 99 3 1 212 3 216 2.85e-70 225
Msa0783850 MtrunA17_Chr3g0118321 30.503 577 324 20 7 549 12 545 1.46e-67 229
Msa0783850 MtrunA17_Chr3g0118331 29.983 597 351 18 1 568 1 559 1.47e-67 229
Msa0783850 MtrunA17_Chr5g0436431 44.958 238 112 5 296 529 6 228 1.59e-60 200
Msa0783850 MtrunA17_Chr8g0374271 27.856 499 305 16 38 531 45 493 1.85e-40 154
Msa0783850 MtrunA17_Chr4g0056511 28.705 533 331 18 11 531 14 509 1.37e-39 152
Msa0783850 MtrunA17_Chr3g0079321 25.690 580 383 20 3 571 9 551 2.26e-38 149
Msa0783850 MtrunA17_Chr4g0024861 26.693 502 300 15 42 531 45 490 5.11e-36 141
Msa0783850 MtrunA17_Chr8g0374261 26.630 552 321 19 7 537 9 497 1.12e-35 140
Msa0783850 MtrunA17_Chr1g0157341 25.646 503 316 14 42 537 47 498 1.13e-34 137
Msa0783850 MtrunA17_Chr5g0402831 27.308 509 292 18 38 531 45 490 1.22e-34 137
Msa0783850 MtrunA17_Chr4g0038671 25.308 569 368 17 8 571 16 532 2.53e-34 136
Msa0783850 MtrunA17_Chr3g0143671 25.787 508 309 16 42 537 48 499 7.26e-34 135
Msa0783850 MtrunA17_Chr2g0322441 26.518 494 311 13 42 531 48 493 4.77e-33 133
Msa0783850 MtrunA17_Chr3g0118351 30.571 350 199 11 240 568 5 331 7.17e-33 129
Msa0783850 MtrunA17_Chr7g0234061 26.806 526 326 18 27 536 32 514 5.91e-31 127
Msa0783850 MtrunA17_Chr3g0121151 24.635 548 348 15 38 571 80 576 8.68e-31 127
Msa0783850 MtrunA17_Chr3g0121171 24.818 548 347 15 38 571 1190 1686 2.19e-30 127
Msa0783850 MtrunA17_Chr3g0121171 24.635 548 348 15 38 571 40 536 8.89e-30 125
Msa0783850 MtrunA17_Chr3g0121171 24.270 548 350 14 38 571 615 1111 2.06e-29 124
Msa0783850 MtrunA17_Chr3g0121171 24.104 502 324 14 80 571 1798 2252 1.63e-24 108
Msa0783850 MtrunA17_Chr3g0121161 25.000 544 351 14 38 571 80 576 3.60e-30 125
Msa0783850 MtrunA17_Chr4g0043261 24.769 541 343 16 11 537 7 497 2.73e-29 122
Msa0783850 MtrunA17_Chr4g0075071 25.836 538 336 20 5 531 9 494 8.75e-28 117
Msa0783850 MtrunA17_Chr4g0043251 25.328 533 355 14 42 571 45 537 4.11e-27 115
Msa0783850 MtrunA17_Chr3g0118341 38.961 154 89 4 1 150 1 153 8.06e-26 105
Msa0783850 MtrunA17_Chr4g0024641 25.108 462 291 17 76 531 1 413 1.00e-20 96.7
Msa0783850 MtrunA17_Chr5g0436411 42.667 75 40 1 8 79 5 79 2.67e-11 59.7
Msa0783850 MtrunA17_Chr3g0118931 36.232 69 44 0 481 549 10 78 8.23e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0783850 AT2G40370.1 75.784 574 126 4 7 571 11 580 0.0 926
Msa0783850 AT5G05390.1 76.399 572 124 3 1 571 4 565 0.0 923
Msa0783850 AT2G30210.1 63.715 576 198 5 1 571 1 570 0.0 790
Msa0783850 AT5G07130.1 65.043 575 191 7 1 571 1 569 0.0 767
Msa0783850 AT5G07130.2 65.102 490 161 7 86 571 1 484 0.0 645
Msa0783850 AT5G07130.3 65.102 490 161 7 86 571 1 484 0.0 645
Msa0783850 AT5G09360.1 51.045 574 262 8 1 571 12 569 0.0 599
Msa0783850 AT2G29130.1 50.943 583 249 12 8 571 9 573 0.0 583
Msa0783850 AT5G60020.1 49.313 582 269 12 7 571 5 577 0.0 564
Msa0783850 AT5G03260.1 49.126 572 264 8 7 571 6 557 0.0 563
Msa0783850 AT5G60020.3 50.817 551 248 11 35 571 4 545 0.0 558
Msa0783850 AT2G38080.1 47.544 570 273 9 7 571 10 558 0.0 558
Msa0783850 AT3G09220.1 49.729 553 254 8 11 554 13 550 0.0 555
Msa0783850 AT5G01190.1 49.561 569 263 7 9 571 8 558 0.0 555
Msa0783850 AT5G58910.2 45.965 570 286 9 8 571 14 567 0.0 540
Msa0783850 AT5G01190.2 51.036 531 238 6 45 571 32 544 0.0 538
Msa0783850 AT5G58910.3 45.965 570 285 10 8 571 14 566 0.0 536
Msa0783850 AT2G46570.1 45.329 578 288 7 4 571 10 569 0.0 526
Msa0783850 AT5G48100.1 46.499 557 278 10 4 555 6 547 0.0 526
Msa0783850 AT1G18140.1 47.957 563 262 13 27 571 32 581 2.43e-179 519
Msa0783850 AT5G01040.1 45.690 580 279 11 5 571 10 566 1.90e-178 516
Msa0783850 AT5G60020.2 49.074 540 249 12 7 529 5 535 8.18e-178 513
Msa0783850 AT5G58910.1 46.239 545 264 10 33 571 2 523 1.45e-177 512
Msa0783850 AT5G01050.1 45.250 579 285 9 5 571 10 568 1.25e-176 512
Msa0783850 AT2G46570.2 46.293 499 242 6 81 571 4 484 1.39e-161 470
Msa0783850 AT3G09220.2 50.214 468 209 8 96 554 1 453 8.78e-161 467
Msa0783850 AT5G21100.1 31.218 583 325 21 19 568 19 558 2.43e-71 239
Msa0783850 AT4G39830.1 30.556 612 339 22 1 571 11 577 3.53e-70 236
Msa0783850 AT5G21105.1 30.442 588 340 19 11 568 25 573 4.75e-67 228
Msa0783850 AT5G21105.3 29.872 549 320 18 11 529 25 538 5.31e-57 200
Msa0783850 AT4G39830.2 29.714 525 286 20 85 571 3 482 3.70e-50 180
Msa0783850 AT4G39830.3 29.580 524 286 20 86 571 2 480 2.44e-49 178
Msa0783850 AT4G39830.4 29.063 523 279 18 85 571 3 469 1.84e-47 172
Msa0783850 AT5G66920.1 26.816 537 333 17 42 571 55 538 8.87e-42 158
Msa0783850 AT4G12420.1 27.387 555 341 21 1 536 1 512 2.02e-41 158
Msa0783850 AT4G12420.2 27.387 555 341 21 1 536 1 512 2.02e-41 158
Msa0783850 AT1G75790.1 26.326 528 344 12 7 531 10 495 1.39e-40 155
Msa0783850 AT5G51480.1 26.232 568 368 17 17 571 21 550 2.99e-39 152
Msa0783850 AT1G21860.1 25.704 533 338 16 11 537 16 496 9.37e-37 144
Msa0783850 AT5G21105.2 29.781 366 215 11 212 568 50 382 1.69e-36 140
Msa0783850 AT1G76160.1 24.624 532 354 15 7 537 11 496 1.70e-36 143
Msa0783850 AT4G25240.2 26.379 580 371 18 8 571 11 550 6.29e-35 139
Msa0783850 AT4G25240.1 26.379 580 371 18 8 571 11 550 6.29e-35 139
Msa0783850 AT1G21850.1 25.373 536 345 20 7 537 11 496 1.61e-33 135
Msa0783850 AT1G41830.1 25.461 542 340 20 6 537 10 497 2.00e-33 134
Msa0783850 AT5G48450.2 25.044 563 372 17 9 568 13 528 1.07e-32 132
Msa0783850 AT5G48450.1 25.044 563 372 17 9 568 13 528 1.59e-32 132
Msa0783850 AT4G22010.1 26.030 534 336 18 6 531 8 490 6.60e-31 127
Msa0783850 AT2G23630.1 27.037 540 329 18 42 571 48 532 5.70e-29 121
Msa0783850 AT2G23630.2 27.037 540 329 18 42 571 53 537 6.08e-29 121
Msa0783850 AT4G37160.1 26.042 576 356 17 10 571 15 534 1.65e-28 120
Msa0783850 AT1G55570.1 25.755 497 319 13 42 531 47 500 3.41e-27 116
Msa0783850 AT3G13390.1 25.194 516 332 12 25 531 29 499 1.52e-26 114
Msa0783850 AT1G55560.1 24.815 540 333 19 42 568 44 523 4.40e-26 112
Msa0783850 AT4G28090.1 23.752 501 325 16 45 537 49 500 1.53e-25 111
Msa0783850 AT3G13400.2 25.646 503 311 16 42 531 45 497 4.30e-24 106
Msa0783850 AT3G13400.1 25.646 503 311 16 42 531 45 497 4.30e-24 106
Msa0783850 AT4G37160.2 29.653 317 201 6 10 323 15 312 2.09e-22 100
Msa0783850 AT4G38420.1 23.092 498 329 15 42 531 48 499 5.16e-21 97.4
Msa0783850 AT4G38420.2 26.939 245 165 5 42 285 48 279 1.81e-18 89.0
Msa0783850 AT4G38420.3 26.939 245 165 5 42 285 48 279 3.17e-18 88.2

Find 157 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAAAAGCTTTAGAAGTTTA+AGG 0.083519 5_2:-68101704 Msa0783850:CDS
ACCTATAGATTTACCATTAA+TGG 0.101466 5_2:-68103170 Msa0783850:CDS
CACCTACTACTGTTAGTTTA+TGG 0.135494 5_2:+68102006 None:intergenic
TGTACCTTCTTGTCCATTAA+TGG 0.180702 5_2:+68103157 None:intergenic
TGGCTTAGAGCCACAGTTTA+TGG 0.186697 5_2:-68103110 Msa0783850:CDS
CAAATGAGCATCACTGTAAA+TGG 0.234006 5_2:-68104137 Msa0783850:CDS
AAAACATATACGCTCATAAT+TGG 0.243066 5_2:+68100213 None:intergenic
CGTGAAACACCCATTCTTCT+AGG 0.253813 5_2:-68103032 Msa0783850:CDS
ACAATTGTTCCAATTGATTC+AGG 0.259031 5_2:-68102095 Msa0783850:CDS
TATATTGTTGCTGAGGGATT+TGG 0.270036 5_2:-68101282 Msa0783850:CDS
ACTGTTGCTGTACCTGTTAA+TGG 0.271612 5_2:-68101198 Msa0783850:CDS
TCTGATGCATACACTATTAA+TGG 0.273121 5_2:-68102534 Msa0783850:CDS
ATTGTTTGTTTCCCTGCATT+GGG 0.279523 5_2:-68100447 Msa0783850:three_prime_UTR
CCAACTTTGGAAATTAATAA+TGG 0.283923 5_2:-68104101 Msa0783850:CDS
AAAGTTACTAAGTTGAGATT+TGG 0.284792 5_2:-68101387 Msa0783850:CDS
GGTGGCATGCTCATAGTTCA+TGG 0.302145 5_2:-68103130 Msa0783850:CDS
TGAGGACCTGCCTTTGTGTT+AGG 0.313647 5_2:-68100517 Msa0783850:exon
GTGCAGCATTGATTACTCTT+AGG 0.316195 5_2:+68102060 None:intergenic
GTGTATGCATCAGATATATT+TGG 0.328938 5_2:+68102543 None:intergenic
CATTGTTTGTTTCCCTGCAT+TGG 0.329323 5_2:-68100448 Msa0783850:three_prime_UTR
CTAGTATCAATTTAACCAAT+AGG 0.331374 5_2:+68100308 None:intergenic
GGACATTGAGTCACAAATTC+TGG 0.334936 5_2:+68103215 None:intergenic
AGATCTTCATCAATTTCATT+AGG 0.335973 5_2:+68101669 None:intergenic
AGAGGATGGAGAAGGGCTAT+TGG 0.338124 5_2:-68100559 Msa0783850:CDS
GCTTGCCTCACAACATCAAT+AGG 0.339702 5_2:+68102585 None:intergenic
TGTTCACATTGGATGGGGTT+TGG 0.346069 5_2:-68100598 Msa0783850:CDS
GTCCTCAGGAGGAGCTTCTA+TGG 0.350489 5_2:+68100533 None:intergenic
AATCACCCTATTCATCTTCA+TGG 0.351556 5_2:-68101315 Msa0783850:CDS
TCATATCCATGAAGATGAAT+AGG 0.353366 5_2:+68101309 None:intergenic
CCATTATTAATTTCCAAAGT+TGG 0.356421 5_2:+68104101 None:intergenic
GTTTGATGTATGTAGGTGTT+TGG 0.364623 5_2:-68100641 Msa0783850:intron
TTTGTTTCTCCTGAATCAAT+TGG 0.365718 5_2:+68102086 None:intergenic
ATTTGTATTGATATGTTATG+AGG 0.373319 5_2:-68100272 Msa0783850:three_prime_UTR
CGCCGGGCGCGCTTACCAAT+CGG 0.373787 5_2:-68101874 Msa0783850:CDS
GGATAAGCATCTCCTTCCTT+AGG 0.376333 5_2:+68103071 None:intergenic
TAGTATCAATTTAACCAATA+GGG 0.376992 5_2:+68100309 None:intergenic
AATTAATAATGGAGATACTT+TGG 0.377624 5_2:-68104090 Msa0783850:CDS
GAGATTTGGATCAAGGGTTC+AGG 0.378810 5_2:-68101373 Msa0783850:CDS
ACAACCAATGTCCTCATGTT+AGG 0.393555 5_2:-68101957 Msa0783850:CDS
ACAATTTCCTGGTCCAACTT+TGG 0.400549 5_2:-68104114 Msa0783850:CDS
ATAACTGCCCAACCATTAAC+AGG 0.400604 5_2:+68101186 None:intergenic
AGAATGGGTGTTTCACGCTT+TGG 0.403573 5_2:+68103038 None:intergenic
CTAAGTTGAGATTTGGATCA+AGG 0.404145 5_2:-68101380 Msa0783850:CDS
TGCTGTACCTGTTAATGGTT+GGG 0.408222 5_2:-68101193 Msa0783850:CDS
TCCATTAATGGTAAATCTAT+AGG 0.411836 5_2:+68103169 None:intergenic
CAATGACCGACTCACATTAC+CGG 0.413180 5_2:+68101431 None:intergenic
AGCCGATTGGTAAGCGCGCC+CGG 0.414132 5_2:+68101872 None:intergenic
ACTGTAAATGGACAATTTCC+TGG 0.419136 5_2:-68104125 Msa0783850:CDS
CCGGCGATGTAGTATCTTGA+TGG 0.420568 5_2:+68101891 None:intergenic
GTTACAATGTCACCATTCAT+TGG 0.426411 5_2:-68104043 Msa0783850:intron
GGATGAGAACATCAGTGGTT+TGG 0.428167 5_2:+68101928 None:intergenic
ATCCTGCAGGCTCATAAACT+TGG 0.437751 5_2:-68101513 Msa0783850:CDS
TTTCATTTAGAGATAAAATT+GGG 0.438549 5_2:-68100389 Msa0783850:three_prime_UTR
CTTGGATGTTCACATTGGAT+GGG 0.442663 5_2:-68100604 Msa0783850:CDS
GATAGTGTCACAAACCCTAT+TGG 0.446797 5_2:-68100323 Msa0783850:three_prime_UTR
ATGATCATTACCTAGAAGAA+TGG 0.449598 5_2:+68103022 None:intergenic
TAACTTTCTCCAGGACGAAT+AGG 0.449717 5_2:+68103194 None:intergenic
ACTTGGATGTTCACATTGGA+TGG 0.450150 5_2:-68100605 Msa0783850:CDS
GCAGGTAATGAAGGCATGAT+TGG 0.454006 5_2:+68101759 None:intergenic
CATATCCATGAAGATGAATA+GGG 0.455044 5_2:+68101310 None:intergenic
GTGAGGCAAGCCACACAAAC+AGG 0.461238 5_2:-68102573 Msa0783850:CDS
GTGTTGCACAGCCTCTTCAC+TGG 0.461801 5_2:+68104161 None:intergenic
ATTATTGTTAATTAGGGGAA+TGG 0.466075 5_2:-68102620 Msa0783850:intron
TCATTGTGGCAACCGATTCA+AGG 0.473488 5_2:-68101414 Msa0783850:CDS
TCATCACCTTGTCCTGCAAA+GGG 0.474167 5_2:-68101798 Msa0783850:CDS
TAACACAAAGGCAGGTCCTC+AGG 0.478994 5_2:+68100519 None:intergenic
TTGCTGTACCTGTTAATGGT+TGG 0.479566 5_2:-68101194 Msa0783850:CDS
TGATGATTTGTATTCAAGAA+TGG 0.480556 5_2:+68101815 None:intergenic
ATCAATGTCAAGGACCGAAC+GGG 0.480946 5_2:-68101593 Msa0783850:CDS
TTGTTAATTAGGGGAATGGT+GGG 0.482575 5_2:-68102616 Msa0783850:intron
GTAACTTTAGTCCCTTGAAT+CGG 0.482864 5_2:+68101402 None:intergenic
AGATTTGTTGCTGACAATCC+AGG 0.489827 5_2:-68101162 Msa0783850:intron
ACCTTGTTGATCCACCTATG+AGG 0.490977 5_2:-68101224 Msa0783850:CDS
ACAGCAACAGTGTTCCTCAT+AGG 0.495749 5_2:+68101210 None:intergenic
TTGGATGTTCACATTGGATG+GGG 0.496559 5_2:-68100603 Msa0783850:CDS
TTGGATTATGCATTGTCACT+TGG 0.496947 5_2:-68100622 Msa0783850:CDS
AACCATAAACTAACAGTAGT+AGG 0.497147 5_2:-68102008 Msa0783850:CDS
GGGGTTAGACAAATCAGAAC+AGG 0.499855 5_2:-68103251 Msa0783850:CDS
CTTGCCTCACAACATCAATA+GGG 0.502090 5_2:+68102586 None:intergenic
ATTGTTAATTAGGGGAATGG+TGG 0.505564 5_2:-68102617 Msa0783850:intron
GATAAGCATCTCCTTCCTTA+GGG 0.506054 5_2:+68103072 None:intergenic
ATGAGGACATTGGTTGTGAA+TGG 0.509668 5_2:+68101963 None:intergenic
TGATCATTACCTAGAAGAAT+GGG 0.510131 5_2:+68103023 None:intergenic
TCACGCTTTGGCTTAGTGAA+AGG 0.510131 5_2:+68103050 None:intergenic
CTCCAAGTTTATGAGCCTGC+AGG 0.510477 5_2:+68101511 None:intergenic
ATCATCACCTTGTCCTGCAA+AGG 0.514426 5_2:-68101799 Msa0783850:CDS
GTGATATTATTACCAATGAA+TGG 0.515384 5_2:+68104031 None:intergenic
GTTAAGGTTGCAAATTGCAA+TGG 0.516063 5_2:+68104438 None:intergenic
TGTGATGTGAATGAAGGTTA+AGG 0.516341 5_2:+68104422 None:intergenic
AGATGAATAGGGTGATTCTC+AGG 0.517564 5_2:+68101321 None:intergenic
ATCAGAACAGGATGGGCTGA+TGG 0.518148 5_2:-68103239 Msa0783850:CDS
TTTGCAATTGTGAAGAAAAG+AGG 0.519484 5_2:+68102029 None:intergenic
ACTCAATGTCCTATTCGTCC+TGG 0.520865 5_2:-68103203 Msa0783850:CDS
GATTATCCTAACACAAAGGC+AGG 0.522293 5_2:+68100511 None:intergenic
GCTCATAAACTTGGAGTACA+AGG 0.524236 5_2:-68101504 Msa0783850:CDS
CAGCATTGATTACTCTTAGG+AGG 0.526575 5_2:+68102063 None:intergenic
GAAGTGAACCGTGTCCCGTT+CGG 0.526770 5_2:+68101579 None:intergenic
TTGATGGTGGTTGGTCACCT+TGG 0.528026 5_2:+68101907 None:intergenic
TTTACCATTAATGGACAAGA+AGG 0.529526 5_2:-68103161 Msa0783850:CDS
GTGTTTGTGTCATTGTAAGC+AGG 0.530023 5_2:+68101741 None:intergenic
TGATTTGCATTAAAATTCGA+CGG 0.538672 5_2:+68101612 None:intergenic
TCATTGTAAGCAGGTAATGA+AGG 0.540128 5_2:+68101750 None:intergenic
TGATTTCTATATTGTTGCTG+AGG 0.540678 5_2:-68101289 Msa0783850:CDS
CTTTACAAGTGCTCTACCAA+AGG 0.542011 5_2:-68102498 Msa0783850:intron
AGAACATCAGTGGTTTGGCC+AGG 0.543220 5_2:+68101933 None:intergenic
TTTAATGCAAATCAATGTCA+AGG 0.544640 5_2:-68101603 Msa0783850:CDS
ATCACCTTGTCCTGCAAAGG+GGG 0.545026 5_2:-68101796 Msa0783850:CDS
CTATAGGTGTAACTTTCTCC+AGG 0.545148 5_2:+68103185 None:intergenic
TAAGTTGAGATTTGGATCAA+GGG 0.547755 5_2:-68101379 Msa0783850:CDS
CCATCAAGATACTACATCGC+CGG 0.549313 5_2:-68101891 Msa0783850:CDS
GATTTCTATATTGTTGCTGA+GGG 0.549381 5_2:-68101288 Msa0783850:CDS
ATTCTTCTAGGTAATGATCA+TGG 0.552433 5_2:-68103020 Msa0783850:intron
TGGAGCTGCTCCTGTTTGTG+TGG 0.553190 5_2:+68102563 None:intergenic
CATTGTGGCAACCGATTCAA+GGG 0.556129 5_2:-68101413 Msa0783850:CDS
ACCGGTGTAATCAAATTGCA+CGG 0.556156 5_2:+68101449 None:intergenic
AGCTCTAATCATTCACCCTA+AGG 0.560869 5_2:-68103087 Msa0783850:CDS
CATCAAGATACTACATCGCC+GGG 0.561153 5_2:-68101890 Msa0783850:CDS
ACCACTGATGTTCTCATCCA+AGG 0.561174 5_2:-68101924 Msa0783850:CDS
TTAATTTCCAAAGTTGGACC+AGG 0.564251 5_2:+68104107 None:intergenic
GACAAGAAGGTACACTTTGG+TGG 0.574088 5_2:-68103148 Msa0783850:CDS
TTGTTTGTTTCCCTGCATTG+GGG 0.580260 5_2:-68100446 Msa0783850:three_prime_UTR
TTGTTTACTACTCTAAACAA+TGG 0.582953 5_2:-68104381 Msa0783850:exon
TACACTATTAATGGTCAACC+TGG 0.583665 5_2:-68102525 Msa0783850:CDS
GTCAAGGACCGAACGGGACA+CGG 0.584236 5_2:-68101587 Msa0783850:CDS
CTAATCATTCACCCTAAGGA+AGG 0.586825 5_2:-68103083 Msa0783850:CDS
TGTAGTATCTTGATGGTGGT+TGG 0.588008 5_2:+68101898 None:intergenic
GGTTGTGAATGGTTTGACAT+AGG 0.595120 5_2:+68101974 None:intergenic
AATGTGTGTGATGTGAATGA+AGG 0.600844 5_2:+68104416 None:intergenic
ATGGACAAGAAGGTACACTT+TGG 0.605647 5_2:-68103151 Msa0783850:CDS
TTTGTATTCAAGAATGGCAG+TGG 0.605780 5_2:+68101821 None:intergenic
ATTGTTGAGTCCTAATCCAA+TGG 0.608848 5_2:+68101638 None:intergenic
TAGACAAATCAGAACAGGAT+GGG 0.611575 5_2:-68103246 Msa0783850:CDS
AATCAATGTCAAGGACCGAA+CGG 0.611682 5_2:-68101594 Msa0783850:CDS
TACAAGCAACTCCAGTGAAG+AGG 0.611814 5_2:-68104172 Msa0783850:CDS
GAGCACTTGTAAAGATCACC+AGG 0.619435 5_2:+68102507 None:intergenic
GCAACAGTGTTCCTCATAGG+TGG 0.621280 5_2:+68101213 None:intergenic
TGTCACTTGGATGTTCACAT+TGG 0.623581 5_2:-68100609 Msa0783850:CDS
GTGTAATCAAATTGCACGGG+AGG 0.625603 5_2:+68101453 None:intergenic
CCGGTGTAATCAAATTGCAC+GGG 0.626025 5_2:+68101450 None:intergenic
AATGTCCTCATGTTAGGACC+TGG 0.628341 5_2:-68101951 Msa0783850:CDS
ATTACACCGGTAATGTGAGT+CGG 0.633330 5_2:-68101437 Msa0783850:CDS
GTCCATAGAAGCTCCTCCTG+AGG 0.638740 5_2:-68100535 Msa0783850:CDS
ACCTTGGATGAGAACATCAG+TGG 0.638752 5_2:+68101923 None:intergenic
CACAAAGGCAGGTCCTCAGG+AGG 0.658358 5_2:+68100522 None:intergenic
GGGTTTGTGACACTATCAGA+TGG 0.658383 5_2:+68100329 None:intergenic
TCACCTTGTCCTGCAAAGGG+GGG 0.659071 5_2:-68101795 Msa0783850:CDS
TTTGGATTATCCTAACACAA+AGG 0.661322 5_2:+68100507 None:intergenic
GTAATGTGAGTCGGTCATTG+TGG 0.663037 5_2:-68101428 Msa0783850:CDS
TCCTCATAGGTGGATCAACA+AGG 0.663984 5_2:+68101223 None:intergenic
CCCGTGCAATTTGATTACAC+CGG 0.670640 5_2:-68101450 Msa0783850:CDS
AGGTCCTAACATGAGGACAT+TGG 0.671705 5_2:+68101953 None:intergenic
CATCACCTTGTCCTGCAAAG+GGG 0.673225 5_2:-68101797 Msa0783850:CDS
TTTGGCCAGGTCCTAACATG+AGG 0.676864 5_2:+68101946 None:intergenic
TTAGACAAATCAGAACAGGA+TGG 0.677485 5_2:-68103247 Msa0783850:CDS
GATTAGAGCTCCATAAACTG+TGG 0.691733 5_2:+68103100 None:intergenic
GCGATGTAGTATCTTGATGG+TGG 0.696908 5_2:+68101894 None:intergenic
CAAACCCTATTGATGTTGTG+AGG 0.706688 5_2:-68102590 Msa0783850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAACAACTAAAAATAAAAA+GGG + chr5_2:68100402-68100421 None:intergenic 10.0%
!! ATTAACAACTAAAAATAAAA+AGG + chr5_2:68100403-68100422 None:intergenic 10.0%
!! ACTATAAAAATACTATTATT+TGG + chr5_2:68101201-68101220 None:intergenic 10.0%
!!! AATAATAGTATTTTTATAGT+AGG - chr5_2:68101201-68101220 Msa0783850:CDS 10.0%
!!! ATAATAGTATTTTTATAGTA+GGG - chr5_2:68101202-68101221 Msa0783850:CDS 10.0%
!!! TTTTTTCAATTTGTTAATTA+AGG - chr5_2:68101812-68101831 Msa0783850:CDS 10.0%
!!! TTTTTTCAATTTGTTAATTA+AGG - chr5_2:68101812-68101831 Msa0783850:CDS 10.0%
!! CAATTAATTAATTATGTATA+TGG + chr5_2:68102379-68102398 None:intergenic 10.0%
!!! ATTTTGAATAAAATTTATGT+AGG + chr5_2:68103617-68103636 None:intergenic 10.0%
!!! GAAAAAATTGTAAATTTTAT+AGG - chr5_2:68103750-68103769 Msa0783850:intron 10.0%
!! AAACTTTATCATAAAAATTA+AGG + chr5_2:68103794-68103813 None:intergenic 10.0%
!!! AATTACAAATTTTCATAATT+TGG + chr5_2:68104163-68104182 None:intergenic 10.0%
!!! TAGTTGTTAATATAACTTTT+AGG - chr5_2:68100412-68100431 Msa0783850:three_prime_UTR 15.0%
!! GGAGATTAATTATAATTAAT+TGG - chr5_2:68100433-68100452 Msa0783850:three_prime_UTR 15.0%
!! TTATATTCCTATTAGTATAT+AGG - chr5_2:68100819-68100838 Msa0783850:intron 15.0%
!!! CAATTAAATTTGTTATTTGA+CGG + chr5_2:68100949-68100968 None:intergenic 15.0%
!! AAAACTAAAAAGTAACTATA+TGG + chr5_2:68101096-68101115 None:intergenic 15.0%
!! TAAAAATACTATTATTTGGA+AGG + chr5_2:68101197-68101216 None:intergenic 15.0%
!!! ACCACAATTTAAAATTTTAA+TGG - chr5_2:68101276-68101295 Msa0783850:CDS 15.0%
!!! ACCATTAAAATTTTAAATTG+TGG + chr5_2:68101280-68101299 None:intergenic 15.0%
!! TGCAAATAATCAATAATTTA+TGG - chr5_2:68101342-68101361 Msa0783850:CDS 15.0%
!! AGATAAGAATTAAAATAAGT+TGG + chr5_2:68101770-68101789 None:intergenic 15.0%
!! TATGATGATTATTGTTAATT+AGG - chr5_2:68102022-68102041 Msa0783850:CDS 15.0%
!! ATGATGATTATTGTTAATTA+GGG - chr5_2:68102023-68102042 Msa0783850:CDS 15.0%
!!! TATATGGTTTTTTTAAATGT+CGG + chr5_2:68102363-68102382 None:intergenic 15.0%
!!! TAAGCAAAAATCAATATTTT+AGG + chr5_2:68102408-68102427 None:intergenic 15.0%
!!! TTTTCATTTAGAGATAAAAT+TGG - chr5_2:68104259-68104278 Msa0783850:intron 15.0%
!! TTTCATTTAGAGATAAAATT+GGG - chr5_2:68104260-68104279 Msa0783850:intron 15.0%
!!! AATTAATAATGGAGATACTT+TGG - chr5_2:68100559-68100578 Msa0783850:CDS 20.0%
!! CGTCAAATAACAAATTTAAT+TGG - chr5_2:68100947-68100966 Msa0783850:intron 20.0%
!! TCGAAAATCAAAAACAAAAA+AGG + chr5_2:68101150-68101169 None:intergenic 20.0%
!! ATGCATGCTATAATTTATAA+AGG - chr5_2:68101309-68101328 Msa0783850:CDS 20.0%
!!! TTATTGCATTTTGAATCTTT+AGG - chr5_2:68101372-68101391 Msa0783850:CDS 20.0%
!! AATCTTTGTCTAAAAAGAAA+AGG + chr5_2:68101965-68101984 None:intergenic 20.0%
!! TGATGATTATTGTTAATTAG+GGG - chr5_2:68102024-68102043 Msa0783850:CDS 20.0%
!! ACATAAAATAATCAAACCTT+TGG + chr5_2:68102170-68102189 None:intergenic 20.0%
!!! TTTTTGCTTATAATAGTGAT+CGG - chr5_2:68102418-68102437 Msa0783850:intron 20.0%
!! AGAATTAAATAAGTGATTGA+AGG + chr5_2:68102465-68102484 None:intergenic 20.0%
!! AATGATCATATGTCAAATAA+TGG + chr5_2:68102519-68102538 None:intergenic 20.0%
!! ATTATTTGACATATGATCAT+TGG - chr5_2:68102518-68102537 Msa0783850:CDS 20.0%
!!! ATTTTTTATACAATGCAAAG+TGG + chr5_2:68103530-68103549 None:intergenic 20.0%
!! TTGAATAAAATTTATGTAGG+AGG + chr5_2:68103614-68103633 None:intergenic 20.0%
!! GTCTCAATAAAATCAAAATA+AGG - chr5_2:68103692-68103711 Msa0783850:intron 20.0%
!! ATGTTTGATCTTATGTAATT+TGG - chr5_2:68103983-68104002 Msa0783850:intron 20.0%
!! TAGTATCAATTTAACCAATA+GGG + chr5_2:68104343-68104362 None:intergenic 20.0%
!!! ATTTGTATTGATATGTTATG+AGG - chr5_2:68104377-68104396 Msa0783850:exon 20.0%
! TTGTTTACTACTCTAAACAA+TGG - chr5_2:68100268-68100287 Msa0783850:three_prime_UTR 25.0%
! AAGAAAAATTGCTAAGAAGA+TGG + chr5_2:68100306-68100325 None:intergenic 25.0%
! CCATTATTAATTTCCAAAGT+TGG + chr5_2:68100551-68100570 None:intergenic 25.0%
!! CCAACTTTGGAAATTAATAA+TGG - chr5_2:68100548-68100567 Msa0783850:CDS 25.0%
! GTGATATTATTACCAATGAA+TGG + chr5_2:68100621-68100640 None:intergenic 25.0%
! TCCTATTAGTATATAGGAAA+AGG - chr5_2:68100825-68100844 Msa0783850:intron 25.0%
!! TCCTTTTCCTATATACTAAT+AGG + chr5_2:68100829-68100848 None:intergenic 25.0%
! TAGTATATAGGAAAAGGAAA+TGG - chr5_2:68100831-68100850 Msa0783850:intron 25.0%
! TATAGATAAATTGCAATGCT+CGG - chr5_2:68101007-68101026 Msa0783850:intron 25.0%
! TTAATGAGTTAAGAAAAGTC+AGG - chr5_2:68101063-68101082 Msa0783850:intron 25.0%
! TAATGAGTTAAGAAAAGTCA+GGG - chr5_2:68101064-68101083 Msa0783850:intron 25.0%
! AAGGTGTAAGAAAACAAAAA+AGG + chr5_2:68101178-68101197 None:intergenic 25.0%
! ACCTATAGATTTACCATTAA+TGG - chr5_2:68101479-68101498 Msa0783850:CDS 25.0%
! TCCATTAATGGTAAATCTAT+AGG + chr5_2:68101483-68101502 None:intergenic 25.0%
! ATTATTGTTAATTAGGGGAA+TGG - chr5_2:68102029-68102048 Msa0783850:CDS 25.0%
! AATGGAAAAATGTTTAGTCA+TGG + chr5_2:68102501-68102520 None:intergenic 25.0%
!! TGATGATTTGTATTCAAGAA+TGG + chr5_2:68102837-68102856 None:intergenic 25.0%
!!! GTAAAAGCTTTAGAAGTTTA+AGG - chr5_2:68102945-68102964 Msa0783850:intron 25.0%
! AGATCTTCATCAATTTCATT+AGG + chr5_2:68102983-68103002 None:intergenic 25.0%
!! TGATTTGCATTAAAATTCGA+CGG + chr5_2:68103040-68103059 None:intergenic 25.0%
! TTTAATGCAAATCAATGTCA+AGG - chr5_2:68103046-68103065 Msa0783850:CDS 25.0%
!! AAAGTTACTAAGTTGAGATT+TGG - chr5_2:68103262-68103281 Msa0783850:intron 25.0%
!!! ATTTTGAAGCATCTTTCTTA+GGG + chr5_2:68103402-68103421 None:intergenic 25.0%
!!! AATTTTGAAGCATCTTTCTT+AGG + chr5_2:68103403-68103422 None:intergenic 25.0%
! AAAGTTTCACAAACATGATA+TGG - chr5_2:68103807-68103826 Msa0783850:intron 25.0%
! AGAAAAATGAAACAGAACAA+TGG + chr5_2:68104240-68104259 None:intergenic 25.0%
! CTAGTATCAATTTAACCAAT+AGG + chr5_2:68104344-68104363 None:intergenic 25.0%
! AAAACATATACGCTCATAAT+TGG + chr5_2:68104439-68104458 None:intergenic 25.0%
! CTTAGCAATTTTTCTTCTCT+TGG - chr5_2:68100310-68100329 Msa0783850:three_prime_UTR 30.0%
GGCATGTTTATTAATGTGAA+AGG - chr5_2:68100454-68100473 Msa0783850:three_prime_UTR 30.0%
! CATTTTGAATCTTTAGGCAT+GGG - chr5_2:68101378-68101397 Msa0783850:CDS 30.0%
! ATTTTGAATCTTTAGGCATG+GGG - chr5_2:68101379-68101398 Msa0783850:CDS 30.0%
! TTTACCATTAATGGACAAGA+AGG - chr5_2:68101488-68101507 Msa0783850:CDS 30.0%
TGATCATTACCTAGAAGAAT+GGG + chr5_2:68101629-68101648 None:intergenic 30.0%
ATGATCATTACCTAGAAGAA+TGG + chr5_2:68101630-68101649 None:intergenic 30.0%
ATTCTTCTAGGTAATGATCA+TGG - chr5_2:68101629-68101648 Msa0783850:CDS 30.0%
GACAAAGATTATGTTCATGA+TGG - chr5_2:68101975-68101994 Msa0783850:CDS 30.0%
GTGTATGCATCAGATATATT+TGG + chr5_2:68102109-68102128 None:intergenic 30.0%
TCTGATGCATACACTATTAA+TGG - chr5_2:68102115-68102134 Msa0783850:intron 30.0%
! ACAATTGTTCCAATTGATTC+AGG - chr5_2:68102554-68102573 Msa0783850:CDS 30.0%
! TTTGTTTCTCCTGAATCAAT+TGG + chr5_2:68102566-68102585 None:intergenic 30.0%
TTTGCAATTGTGAAGAAAAG+AGG + chr5_2:68102623-68102642 None:intergenic 30.0%
AACCATAAACTAACAGTAGT+AGG - chr5_2:68102641-68102660 Msa0783850:intron 30.0%
! GAAGATCTTTTCTTTACCAT+TGG - chr5_2:68102995-68103014 Msa0783850:intron 30.0%
! CTTTTCTTTACCATTGGATT+AGG - chr5_2:68103001-68103020 Msa0783850:intron 30.0%
! TAAGTTGAGATTTGGATCAA+GGG - chr5_2:68103270-68103289 Msa0783850:intron 30.0%
CATATCCATGAAGATGAATA+GGG + chr5_2:68103342-68103361 None:intergenic 30.0%
TCATATCCATGAAGATGAAT+AGG + chr5_2:68103343-68103362 None:intergenic 30.0%
TGATTTCTATATTGTTGCTG+AGG - chr5_2:68103360-68103379 Msa0783850:intron 30.0%
GATTTCTATATTGTTGCTGA+GGG - chr5_2:68103361-68103380 Msa0783850:intron 30.0%
ATCAAAATAAGGACTGAAAG+TGG - chr5_2:68103703-68103722 Msa0783850:intron 30.0%
TTTGGTTGTTTGATGTATGT+AGG - chr5_2:68104001-68104020 Msa0783850:intron 30.0%
TTTGGATTATCCTAACACAA+AGG + chr5_2:68104145-68104164 None:intergenic 30.0%
TGTGATGTGAATGAAGGTTA+AGG + chr5_2:68100230-68100249 None:intergenic 35.0%
AATGTGTGTGATGTGAATGA+AGG + chr5_2:68100236-68100255 None:intergenic 35.0%
CAAATGAGCATCACTGTAAA+TGG - chr5_2:68100512-68100531 Msa0783850:exon 35.0%
ACTGTAAATGGACAATTTCC+TGG - chr5_2:68100524-68100543 Msa0783850:CDS 35.0%
TTAATTTCCAAAGTTGGACC+AGG + chr5_2:68100545-68100564 None:intergenic 35.0%
GTTACAATGTCACCATTCAT+TGG - chr5_2:68100606-68100625 Msa0783850:CDS 35.0%
!! AAAAGTGTTTGCTTGCTTGT+GGG + chr5_2:68100749-68100768 None:intergenic 35.0%
!! AAAAAGTGTTTGCTTGCTTG+TGG + chr5_2:68100750-68100769 None:intergenic 35.0%
TCTTAGTACAAGTGTGAAGA+AGG + chr5_2:68100777-68100796 None:intergenic 35.0%
TATTTGACGGAAATCAAGAG+AGG + chr5_2:68100936-68100955 None:intergenic 35.0%
ATAAATTGCAATGCTCGGAA+AGG - chr5_2:68101012-68101031 Msa0783850:intron 35.0%
TAAATTGCAATGCTCGGAAA+GGG - chr5_2:68101013-68101032 Msa0783850:intron 35.0%
AAAAGGAATGTCACTCTACA+TGG + chr5_2:68101133-68101152 None:intergenic 35.0%
! GCATTTTGAATCTTTAGGCA+TGG - chr5_2:68101377-68101396 Msa0783850:CDS 35.0%
TTAGACAAATCAGAACAGGA+TGG - chr5_2:68101402-68101421 Msa0783850:CDS 35.0%
TAGACAAATCAGAACAGGAT+GGG - chr5_2:68101403-68101422 Msa0783850:CDS 35.0%
TGTACCTTCTTGTCCATTAA+TGG + chr5_2:68101495-68101514 None:intergenic 35.0%
! GATTATGTTCATGATGGAGA+TGG - chr5_2:68101981-68102000 Msa0783850:CDS 35.0%
! TCATGATGGAGATGGTTTAA+CGG - chr5_2:68101989-68102008 Msa0783850:CDS 35.0%
ATTGTTAATTAGGGGAATGG+TGG - chr5_2:68102032-68102051 Msa0783850:CDS 35.0%
TTGTTAATTAGGGGAATGGT+GGG - chr5_2:68102033-68102052 Msa0783850:CDS 35.0%
TACACTATTAATGGTCAACC+TGG - chr5_2:68102124-68102143 Msa0783850:intron 35.0%
TTGCTTATAATAGTGATCGG+AGG - chr5_2:68102421-68102440 Msa0783850:intron 35.0%
! CACCTACTACTGTTAGTTTA+TGG + chr5_2:68102646-68102665 None:intergenic 35.0%
TTTGTATTCAAGAATGGCAG+TGG + chr5_2:68102831-68102850 None:intergenic 35.0%
TCATTGTAAGCAGGTAATGA+AGG + chr5_2:68102902-68102921 None:intergenic 35.0%
ATTGTTGAGTCCTAATCCAA+TGG + chr5_2:68103014-68103033 None:intergenic 35.0%
TGCAGGATTGAAAAATTGCT+AGG + chr5_2:68103124-68103143 None:intergenic 35.0%
!! TAGCAATTTTTCAATCCTGC+AGG - chr5_2:68103123-68103142 Msa0783850:CDS 35.0%
!! AGTACAAGGTGTTTTTACGA+CGG - chr5_2:68103159-68103178 Msa0783850:CDS 35.0%
GTAACTTTAGTCCCTTGAAT+CGG + chr5_2:68103250-68103269 None:intergenic 35.0%
! CTAAGTTGAGATTTGGATCA+AGG - chr5_2:68103269-68103288 Msa0783850:intron 35.0%
AATCACCCTATTCATCTTCA+TGG - chr5_2:68103334-68103353 Msa0783850:intron 35.0%
TATATTGTTGCTGAGGGATT+TGG - chr5_2:68103367-68103386 Msa0783850:intron 35.0%
GAAATTGAGCTAATGTTACC+TGG + chr5_2:68103508-68103527 None:intergenic 35.0%
GAGACTAAGTACATGACTAA+AGG + chr5_2:68103677-68103696 None:intergenic 35.0%
GCAGTCAAATGAAATTGATG+TGG + chr5_2:68103878-68103897 None:intergenic 35.0%
GTTTGATGTATGTAGGTGTT+TGG - chr5_2:68104008-68104027 Msa0783850:intron 35.0%
TTGGATTATGCATTGTCACT+TGG - chr5_2:68104027-68104046 Msa0783850:intron 35.0%
!!! ACTGTATTTTTGGTAGAGGA+TGG - chr5_2:68104076-68104095 Msa0783850:CDS 35.0%
ATTGTTTGTTTCCCTGCATT+GGG - chr5_2:68104202-68104221 Msa0783850:intron 35.0%
GAAAAAAAATCCCCAATGCA+GGG + chr5_2:68104216-68104235 None:intergenic 35.0%
AGAAAAAAAATCCCCAATGC+AGG + chr5_2:68104217-68104236 None:intergenic 35.0%
ACAATTTCCTGGTCCAACTT+TGG - chr5_2:68100535-68100554 Msa0783850:CDS 40.0%
ACGGAAATCAAGAGAGGTAA+TGG + chr5_2:68100930-68100949 None:intergenic 40.0%
GGACATTGAGTCACAAATTC+TGG + chr5_2:68101437-68101456 None:intergenic 40.0%
TAACTTTCTCCAGGACGAAT+AGG + chr5_2:68101458-68101477 None:intergenic 40.0%
CTATAGGTGTAACTTTCTCC+AGG + chr5_2:68101467-68101486 None:intergenic 40.0%
! ATGGACAAGAAGGTACACTT+TGG - chr5_2:68101498-68101517 Msa0783850:CDS 40.0%
GATTAGAGCTCCATAAACTG+TGG + chr5_2:68101552-68101571 None:intergenic 40.0%
! AGCTCTAATCATTCACCCTA+AGG - chr5_2:68101562-68101581 Msa0783850:CDS 40.0%
CTAATCATTCACCCTAAGGA+AGG - chr5_2:68101566-68101585 Msa0783850:CDS 40.0%
GATAAGCATCTCCTTCCTTA+GGG + chr5_2:68101580-68101599 None:intergenic 40.0%
CAAACCCTATTGATGTTGTG+AGG - chr5_2:68102059-68102078 Msa0783850:CDS 40.0%
CTTGCCTCACAACATCAATA+GGG + chr5_2:68102066-68102085 None:intergenic 40.0%
CTTTACAAGTGCTCTACCAA+AGG - chr5_2:68102151-68102170 Msa0783850:intron 40.0%
CATATGATCATTGGCAATGC+AGG - chr5_2:68102527-68102546 Msa0783850:CDS 40.0%
!! CAGCATTGATTACTCTTAGG+AGG + chr5_2:68102589-68102608 None:intergenic 40.0%
!! GTGCAGCATTGATTACTCTT+AGG + chr5_2:68102592-68102611 None:intergenic 40.0%
GGTTGTGAATGGTTTGACAT+AGG + chr5_2:68102678-68102697 None:intergenic 40.0%
! ATGAGGACATTGGTTGTGAA+TGG + chr5_2:68102689-68102708 None:intergenic 40.0%
ACAACCAATGTCCTCATGTT+AGG - chr5_2:68102692-68102711 Msa0783850:intron 40.0%
!! TGTAGTATCTTGATGGTGGT+TGG + chr5_2:68102754-68102773 None:intergenic 40.0%
! GTGTTTGTGTCATTGTAAGC+AGG + chr5_2:68102911-68102930 None:intergenic 40.0%
AATCAATGTCAAGGACCGAA+CGG - chr5_2:68103055-68103074 Msa0783850:CDS 40.0%
GCTCATAAACTTGGAGTACA+AGG - chr5_2:68103145-68103164 Msa0783850:CDS 40.0%
ACCGGTGTAATCAAATTGCA+CGG + chr5_2:68103203-68103222 None:intergenic 40.0%
ATTACACCGGTAATGTGAGT+CGG - chr5_2:68103212-68103231 Msa0783850:CDS 40.0%
AGATGAATAGGGTGATTCTC+AGG + chr5_2:68103331-68103350 None:intergenic 40.0%
ACTGTTGCTGTACCTGTTAA+TGG - chr5_2:68103451-68103470 Msa0783850:intron 40.0%
TTGCTGTACCTGTTAATGGT+TGG - chr5_2:68103455-68103474 Msa0783850:intron 40.0%
TGCTGTACCTGTTAATGGTT+GGG - chr5_2:68103456-68103475 Msa0783850:intron 40.0%
ATAACTGCCCAACCATTAAC+AGG + chr5_2:68103466-68103485 None:intergenic 40.0%
!! AGATTTGTTGCTGACAATCC+AGG - chr5_2:68103487-68103506 Msa0783850:intron 40.0%
! TGTTTCTGTGACCATAACTG+AGG - chr5_2:68103852-68103871 Msa0783850:intron 40.0%
AATTGATGTGGCCTCAGTTA+TGG + chr5_2:68103866-68103885 None:intergenic 40.0%
CAGGACCAAGTATCTATATG+CGG - chr5_2:68103907-68103926 Msa0783850:intron 40.0%
GCGGTTGCGATATAAAACTT+TGG - chr5_2:68103926-68103945 Msa0783850:intron 40.0%
! TGTCACTTGGATGTTCACAT+TGG - chr5_2:68104040-68104059 Msa0783850:intron 40.0%
ACTTGGATGTTCACATTGGA+TGG - chr5_2:68104044-68104063 Msa0783850:CDS 40.0%
CTTGGATGTTCACATTGGAT+GGG - chr5_2:68104045-68104064 Msa0783850:CDS 40.0%
TTGGATGTTCACATTGGATG+GGG - chr5_2:68104046-68104065 Msa0783850:CDS 40.0%
! GGGTTTGGCTACTGTATTTT+TGG - chr5_2:68104066-68104085 Msa0783850:CDS 40.0%
!!! GGCTACTGTATTTTTGGTAG+AGG - chr5_2:68104072-68104091 Msa0783850:CDS 40.0%
!! TTTTTGGTAGAGGATGGAGA+AGG - chr5_2:68104082-68104101 Msa0783850:CDS 40.0%
! TTTTGGTAGAGGATGGAGAA+GGG - chr5_2:68104083-68104102 Msa0783850:CDS 40.0%
GATTATCCTAACACAAAGGC+AGG + chr5_2:68104141-68104160 None:intergenic 40.0%
CATTGTTTGTTTCCCTGCAT+TGG - chr5_2:68104201-68104220 Msa0783850:intron 40.0%
TTGTTTGTTTCCCTGCATTG+GGG - chr5_2:68104203-68104222 Msa0783850:intron 40.0%
GATAGTGTCACAAACCCTAT+TGG - chr5_2:68104326-68104345 Msa0783850:CDS 40.0%
TACAAGCAACTCCAGTGAAG+AGG - chr5_2:68100477-68100496 Msa0783850:three_prime_UTR 45.0%
GGGGTTAGACAAATCAGAAC+AGG - chr5_2:68101398-68101417 Msa0783850:CDS 45.0%
ACTCAATGTCCTATTCGTCC+TGG - chr5_2:68101446-68101465 Msa0783850:CDS 45.0%
! GACAAGAAGGTACACTTTGG+TGG - chr5_2:68101501-68101520 Msa0783850:CDS 45.0%
TGGCTTAGAGCCACAGTTTA+TGG - chr5_2:68101539-68101558 Msa0783850:CDS 45.0%
GGATAAGCATCTCCTTCCTT+AGG + chr5_2:68101581-68101600 None:intergenic 45.0%
! TCACGCTTTGGCTTAGTGAA+AGG + chr5_2:68101602-68101621 None:intergenic 45.0%
AGAATGGGTGTTTCACGCTT+TGG + chr5_2:68101614-68101633 None:intergenic 45.0%
CGTGAAACACCCATTCTTCT+AGG - chr5_2:68101617-68101636 Msa0783850:CDS 45.0%
GCTTGCCTCACAACATCAAT+AGG + chr5_2:68102067-68102086 None:intergenic 45.0%
GAGCACTTGTAAAGATCACC+AGG + chr5_2:68102145-68102164 None:intergenic 45.0%
AGGTCCTAACATGAGGACAT+TGG + chr5_2:68102699-68102718 None:intergenic 45.0%
AATGTCCTCATGTTAGGACC+TGG - chr5_2:68102698-68102717 Msa0783850:intron 45.0%
GGATGAGAACATCAGTGGTT+TGG + chr5_2:68102724-68102743 None:intergenic 45.0%
ACCACTGATGTTCTCATCCA+AGG - chr5_2:68102725-68102744 Msa0783850:intron 45.0%
ACCTTGGATGAGAACATCAG+TGG + chr5_2:68102729-68102748 None:intergenic 45.0%
GCGATGTAGTATCTTGATGG+TGG + chr5_2:68102758-68102777 None:intergenic 45.0%
CCATCAAGATACTACATCGC+CGG - chr5_2:68102758-68102777 Msa0783850:intron 45.0%
CATCAAGATACTACATCGCC+GGG - chr5_2:68102759-68102778 Msa0783850:intron 45.0%
ATCATCACCTTGTCCTGCAA+AGG - chr5_2:68102850-68102869 Msa0783850:intron 45.0%
TCATCACCTTGTCCTGCAAA+GGG - chr5_2:68102851-68102870 Msa0783850:intron 45.0%
! GCAGGTAATGAAGGCATGAT+TGG + chr5_2:68102893-68102912 None:intergenic 45.0%
ATCAATGTCAAGGACCGAAC+GGG - chr5_2:68103056-68103075 Msa0783850:CDS 45.0%
ATCCTGCAGGCTCATAAACT+TGG - chr5_2:68103136-68103155 Msa0783850:CDS 45.0%
!! AATTGCACGGGAGGTTTTGT+GGG + chr5_2:68103190-68103209 None:intergenic 45.0%
! AAATTGCACGGGAGGTTTTG+TGG + chr5_2:68103191-68103210 None:intergenic 45.0%
GTGTAATCAAATTGCACGGG+AGG + chr5_2:68103199-68103218 None:intergenic 45.0%
CCGGTGTAATCAAATTGCAC+GGG + chr5_2:68103202-68103221 None:intergenic 45.0%
CCCGTGCAATTTGATTACAC+CGG - chr5_2:68103199-68103218 Msa0783850:CDS 45.0%
! CAATGACCGACTCACATTAC+CGG + chr5_2:68103221-68103240 None:intergenic 45.0%
GTAATGTGAGTCGGTCATTG+TGG - chr5_2:68103221-68103240 Msa0783850:CDS 45.0%
! TCATTGTGGCAACCGATTCA+AGG - chr5_2:68103235-68103254 Msa0783850:CDS 45.0%
! CATTGTGGCAACCGATTCAA+GGG - chr5_2:68103236-68103255 Msa0783850:CDS 45.0%
GAGATTTGGATCAAGGGTTC+AGG - chr5_2:68103276-68103295 Msa0783850:intron 45.0%
ACCTTGTTGATCCACCTATG+AGG - chr5_2:68103425-68103444 Msa0783850:intron 45.0%
TCCTCATAGGTGGATCAACA+AGG + chr5_2:68103429-68103448 None:intergenic 45.0%
ACAGCAACAGTGTTCCTCAT+AGG + chr5_2:68103442-68103461 None:intergenic 45.0%
ATTTGACTGCGATTCTCTGC+AGG - chr5_2:68103888-68103907 Msa0783850:intron 45.0%
CGCAACCGCATATAGATACT+TGG + chr5_2:68103915-68103934 None:intergenic 45.0%
TGTTCACATTGGATGGGGTT+TGG - chr5_2:68104051-68104070 Msa0783850:CDS 45.0%
GGGTTTGTGACACTATCAGA+TGG + chr5_2:68104323-68104342 None:intergenic 45.0%
!! ATTGTATAAAAAATATTATT+AGG - chr5_2:68103536-68103555 Msa0783850:intron 5.0%
!! TTGTATAAAAAATATTATTA+GGG - chr5_2:68103537-68103556 Msa0783850:intron 5.0%
!! TGTAAAAATTAATAAAAATA+GGG + chr5_2:68103644-68103663 None:intergenic 5.0%
!! TTGTAAAAATTAATAAAAAT+AGG + chr5_2:68103645-68103664 None:intergenic 5.0%
!!! TTTTATTAATTTTTACAAAA+TGG - chr5_2:68103647-68103666 Msa0783850:intron 5.0%
!!! AAAAAATTGTAAATTTTATA+GGG - chr5_2:68103751-68103770 Msa0783850:intron 5.0%
!! ATCAGAACAGGATGGGCTGA+TGG - chr5_2:68101410-68101429 Msa0783850:CDS 50.0%
GGTGGCATGCTCATAGTTCA+TGG - chr5_2:68101519-68101538 Msa0783850:CDS 50.0%
TTTGGCCAGGTCCTAACATG+AGG + chr5_2:68102706-68102725 None:intergenic 50.0%
! AGAACATCAGTGGTTTGGCC+AGG + chr5_2:68102719-68102738 None:intergenic 50.0%
!! TTGATGGTGGTTGGTCACCT+TGG + chr5_2:68102745-68102764 None:intergenic 50.0%
CCGGCGATGTAGTATCTTGA+TGG + chr5_2:68102761-68102780 None:intergenic 50.0%
CATCACCTTGTCCTGCAAAG+GGG - chr5_2:68102852-68102871 Msa0783850:intron 50.0%
ATCACCTTGTCCTGCAAAGG+GGG - chr5_2:68102853-68102872 Msa0783850:intron 50.0%
ATATTTGCACCCCCCTTTGC+AGG + chr5_2:68102866-68102885 None:intergenic 50.0%
CTCCAAGTTTATGAGCCTGC+AGG + chr5_2:68103141-68103160 None:intergenic 50.0%
GCAACAGTGTTCCTCATAGG+TGG + chr5_2:68103439-68103458 None:intergenic 50.0%
AGAGGATGGAGAAGGGCTAT+TGG - chr5_2:68104090-68104109 Msa0783850:CDS 50.0%
TAACACAAAGGCAGGTCCTC+AGG + chr5_2:68104133-68104152 None:intergenic 50.0%
TGAGGACCTGCCTTTGTGTT+AGG - chr5_2:68104132-68104151 Msa0783850:CDS 50.0%
GTGTTGCACAGCCTCTTCAC+TGG + chr5_2:68100491-68100510 None:intergenic 55.0%
GTGAGGCAAGCCACACAAAC+AGG - chr5_2:68102076-68102095 Msa0783850:CDS 55.0%
TGGAGCTGCTCCTGTTTGTG+TGG + chr5_2:68102089-68102108 None:intergenic 55.0%
!! ACGCAGCGTTTTGAGCCGAT+TGG + chr5_2:68102793-68102812 None:intergenic 55.0%
TCACCTTGTCCTGCAAAGGG+GGG - chr5_2:68102854-68102873 Msa0783850:intron 55.0%
GAAGTGAACCGTGTCCCGTT+CGG + chr5_2:68103073-68103092 None:intergenic 55.0%
GTCCATAGAAGCTCCTCCTG+AGG - chr5_2:68104114-68104133 Msa0783850:CDS 55.0%
GTCCTCAGGAGGAGCTTCTA+TGG + chr5_2:68104119-68104138 None:intergenic 55.0%
GTCAAGGACCGAACGGGACA+CGG - chr5_2:68103062-68103081 Msa0783850:CDS 60.0%
CACAAAGGCAGGTCCTCAGG+AGG + chr5_2:68104130-68104149 None:intergenic 60.0%
AGCCGATTGGTAAGCGCGCC+CGG + chr5_2:68102780-68102799 None:intergenic 65.0%
GCACCCCCCTTTGCAGGACA+AGG + chr5_2:68102860-68102879 None:intergenic 65.0%
CGCCGGGCGCGCTTACCAAT+CGG - chr5_2:68102775-68102794 Msa0783850:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 68100213 68104458 68100213 ID=Msa0783850;Name=Msa0783850
chr5_2 mRNA 68100213 68104458 68100213 ID=Msa0783850-mRNA-1;Parent=Msa0783850;Name=Msa0783850-mRNA-1;_AED=0.05;_eAED=0.05;_QI=74|0.8|0.83|1|1|1|6|305|571
chr5_2 exon 68100213 68100648 68100213 ID=Msa0783850-mRNA-1:exon:20414;Parent=Msa0783850-mRNA-1
chr5_2 exon 68101163 68102122 68101163 ID=Msa0783850-mRNA-1:exon:20413;Parent=Msa0783850-mRNA-1
chr5_2 exon 68102499 68102627 68102499 ID=Msa0783850-mRNA-1:exon:20412;Parent=Msa0783850-mRNA-1
chr5_2 exon 68103021 68103277 68103021 ID=Msa0783850-mRNA-1:exon:20411;Parent=Msa0783850-mRNA-1
chr5_2 exon 68104044 68104195 68104044 ID=Msa0783850-mRNA-1:exon:20410;Parent=Msa0783850-mRNA-1
chr5_2 exon 68104298 68104458 68104298 ID=Msa0783850-mRNA-1:exon:20409;Parent=Msa0783850-mRNA-1
chr5_2 five_prime_UTR 68104385 68104458 68104385 ID=Msa0783850-mRNA-1:five_prime_utr;Parent=Msa0783850-mRNA-1
chr5_2 CDS 68104298 68104384 68104298 ID=Msa0783850-mRNA-1:cds;Parent=Msa0783850-mRNA-1
chr5_2 CDS 68104044 68104195 68104044 ID=Msa0783850-mRNA-1:cds;Parent=Msa0783850-mRNA-1
chr5_2 CDS 68103021 68103277 68103021 ID=Msa0783850-mRNA-1:cds;Parent=Msa0783850-mRNA-1
chr5_2 CDS 68102499 68102627 68102499 ID=Msa0783850-mRNA-1:cds;Parent=Msa0783850-mRNA-1
chr5_2 CDS 68101163 68102122 68101163 ID=Msa0783850-mRNA-1:cds;Parent=Msa0783850-mRNA-1
chr5_2 CDS 68100518 68100648 68100518 ID=Msa0783850-mRNA-1:cds;Parent=Msa0783850-mRNA-1
chr5_2 three_prime_UTR 68100213 68100517 68100213 ID=Msa0783850-mRNA-1:three_prime_utr;Parent=Msa0783850-mRNA-1
Gene Sequence

>Msa0783850

ATGGAAGCTTTCAACACCATCTTCTTAGCAATTTTTCTTCTCTTGGCTTCTTTAGCTAATGCTAAAGTTCATGAGCATGAGTTTGTCGTACAAGCAACTCCAGTGAAGAGGCTGTGCAACACACAAATGAGCATCACTGTAAATGGACAATTTCCTGGTCCAACTTTGGAAATTAATAATGGAGATACTTTGGTTGTCAAAGTCATTAACAAAGCTCGTTACAATGTCACCATTCATTGGCATGGGGTTAGACAAATCAGAACAGGATGGGCTGATGGACCAGAATTTGTGACTCAATGTCCTATTCGTCCTGGAGAAAGTTACACCTATAGATTTACCATTAATGGACAAGAAGGTACACTTTGGTGGCATGCTCATAGTTCATGGCTTAGAGCCACAGTTTATGGAGCTCTAATCATTCACCCTAAGGAAGGAGATGCTTATCCTTTCACTAAGCCAAAGCGTGAAACACCCATTCTTCTAGGTAATGATCATGGGGAATGGTGGGATGCAAACCCTATTGATGTTGTGAGGCAAGCCACACAAACAGGAGCAGCTCCAAATATATCTGATGCATACACTATTAATGGTCAACCTGGTGATCTTTACAAGTGCTCTACCAAAGGCACAACAATTGTTCCAATTGATTCAGGAGAAACAAACCTCCTAAGAGTAATCAATGCTGCACTAAATCAACCTCTTTTCTTCACAATTGCAAACCATAAACTAACAGTAGTAGGTGCTGATGCTTCCTATGTCAAACCATTCACAACCAATGTCCTCATGTTAGGACCTGGCCAAACCACTGATGTTCTCATCCAAGGTGACCAACCACCATCAAGATACTACATCGCCGGGCGCGCTTACCAATCGGCTCAAAACGCTGCGTTCGATAACACAACAACCACTGCCATTCTTGAATACAAATCATCACCTTGTCCTGCAAAGGGGGGTGCAAATATCAGACCAATCATGCCTTCATTACCTGCTTACAATGACACAAACACTGTTACATCATTTAGTAAAAGCTTTAGAAGTTTAAGGAATGTTGAAGTTCCTAATGAAATTGATGAAGATCTTTTCTTTACCATTGGATTAGGACTCAACAATTGTCCGTCGAATTTTAATGCAAATCAATGTCAAGGACCGAACGGGACACGGTTCACTTCTAGTATGAACAATGTGTCTTTTGTACTTCCTAGCAATTTTTCAATCCTGCAGGCTCATAAACTTGGAGTACAAGGTGTTTTTACGACGGATTTTCCCACAAAACCTCCCGTGCAATTTGATTACACCGGTAATGTGAGTCGGTCATTGTGGCAACCGATTCAAGGGACTAAAGTTACTAAGTTGAGATTTGGATCAAGGGTTCAGGTTGTTTTGCAAGATACTAGTATTGTTACTCCTGAGAATCACCCTATTCATCTTCATGGATATGATTTCTATATTGTTGCTGAGGGATTTGGAAATTATGACCCTAAGAAAGATGCTTCAAAATTTAACCTTGTTGATCCACCTATGAGGAACACTGTTGCTGTACCTGTTAATGGTTGGGCAGTTATCAGATTTGTTGCTGACAATCCAGGTGTTTGGATTATGCATTGTCACTTGGATGTTCACATTGGATGGGGTTTGGCTACTGTATTTTTGGTAGAGGATGGAGAAGGGCTATTGGAGTCCATAGAAGCTCCTCCTGAGGACCTGCCTTTGTGTTAG
Protein sequence

>Msa0783850

MEAFNTIFLAIFLLLASLANAKVHEHEFVVQATPVKRLCNTQMSITVNGQFPGPTLEINNGDTLVVKVINKARYNVTIHWHGVRQIRTGWADGPEFVTQCPIRPGESYTYRFTINGQEGTLWWHAHSSWLRATVYGALIIHPKEGDAYPFTKPKRETPILLGNDHGEWWDANPIDVVRQATQTGAAPNISDAYTINGQPGDLYKCSTKGTTIVPIDSGETNLLRVINAALNQPLFFTIANHKLTVVGADASYVKPFTTNVLMLGPGQTTDVLIQGDQPPSRYYIAGRAYQSAQNAAFDNTTTTAILEYKSSPCPAKGGANIRPIMPSLPAYNDTNTVTSFSKSFRSLRNVEVPNEIDEDLFFTIGLGLNNCPSNFNANQCQGPNGTRFTSSMNNVSFVLPSNFSILQAHKLGVQGVFTTDFPTKPPVQFDYTGNVSRSLWQPIQGTKVTKLRFGSRVQVVLQDTSIVTPENHPIHLHGYDFYIVAEGFGNYDPKKDASKFNLVDPPMRNTVAVPVNGWAVIRFVADNPGVWIMHCHLDVHIGWGLATVFLVEDGEGLLESIEAPPEDLPLC*