AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1191100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 290 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGGGAGTGGGATATTATT+TGG 0.132396 8_1:-34865703 Msa1191100:CDS
TTGATATAAGAATCTGTAAT+TGG 0.259531 8_1:+34865392 None:intergenic
GTGGGAGTGGGATATTATTT+GGG 0.326577 8_1:-34865702 Msa1191100:CDS
TGGGATATTCCTAAGTGTTC+AGG 0.338735 8_1:-34866067 Msa1191100:intron
CTCTTCAAATGAGATGAAAA+TGG 0.341128 8_1:+34865520 None:intergenic
ATGGCTTTGGTAGCATCTTC+TGG 0.345171 8_1:+34865625 None:intergenic
CTTTCTTCTCGTTTGAAGAT+AGG 0.345464 8_1:-34865588 Msa1191100:CDS
TGCTGATGTTGTTTCAAGCT+TGG 0.353482 8_1:-34867030 Msa1191100:five_prime_UTR
AATACTCCTTTCCAAGCAAA+TGG 0.388315 8_1:+34867087 None:intergenic
AGCAATGAGGATGTTACTAT+TGG 0.392019 8_1:-34866175 Msa1191100:five_prime_UTR
ACTATACTAATATATCTTGC+AGG 0.397277 8_1:-34867124 Msa1191100:five_prime_UTR
CCGATGATGATTAGAATTTG+TGG 0.443752 8_1:-34865721 Msa1191100:CDS
CATCCTCATTGCTGAACATC+CGG 0.452525 8_1:+34866185 None:intergenic
TCAGCAGAAAGAGCATATAT+AGG 0.474635 8_1:+34867047 None:intergenic
TGAACGGGAAAGAATGGCTT+TGG 0.477669 8_1:+34865612 None:intergenic
ATGATTAGAATTTGTGGGAG+TGG 0.489786 8_1:-34865715 Msa1191100:CDS
TCATCATCGGATTCCAGCGT+TGG 0.490644 8_1:+34865734 None:intergenic
AAACGAGAAGAAAGGTGAAC+GGG 0.493600 8_1:+34865597 None:intergenic
CGATGATGATTAGAATTTGT+GGG 0.498077 8_1:-34865720 Msa1191100:CDS
GAGCCACTATCCCATTTGCT+TGG 0.498244 8_1:-34867098 Msa1191100:five_prime_UTR
TTTCCAAGCAAATGGGATAG+TGG 0.498956 8_1:+34867095 None:intergenic
GAAAGGTGAACGGGAAAGAA+TGG 0.499324 8_1:+34865606 None:intergenic
AATGTTGGAGCTCCATCAAA+AGG 0.505564 8_1:-34865774 Msa1191100:CDS
CATCATCGGATTCCAGCGTT+GGG 0.512350 8_1:+34865735 None:intergenic
GGTAGTATTGAATGATATGA+AGG 0.521912 8_1:-34865497 Msa1191100:CDS
AAAAGTAAGCTCAGAAATTG+TGG 0.526614 8_1:-34865679 Msa1191100:CDS
ATACTCCTTTCCAAGCAAAT+GGG 0.531805 8_1:+34867088 None:intergenic
GGCATGAGTCACGGTTTCCA+AGG 0.535403 8_1:-34865305 None:intergenic
TCACTCCTTGGCATGAGTCA+CGG 0.539184 8_1:-34865314 Msa1191100:three_prime_UTR
TGATTAGAATTTGTGGGAGT+GGG 0.541215 8_1:-34865714 Msa1191100:CDS
CTGCTCTCAGAGCCCAACGC+TGG 0.545174 8_1:-34865747 Msa1191100:CDS
ACTATCCCATTTGCTTGGAA+AGG 0.545609 8_1:-34867093 Msa1191100:five_prime_UTR
TGGTTGTTCTTCGAAATGAC+AGG 0.557918 8_1:-34867010 Msa1191100:intron
AGCCACATCTATTGCTGTGT+GGG 0.559911 8_1:-34866086 Msa1191100:CDS
TAATCCAGAAAAGAAAATGT+TGG 0.570821 8_1:-34865789 Msa1191100:CDS
ATATGAAGGTTGTATGAGGA+GGG 0.571801 8_1:-34865483 Msa1191100:exon
CTATCTTCAAACGAGAAGAA+AGG 0.571860 8_1:+34865589 None:intergenic
AGGATGTTACTATTGGAGCT+TGG 0.572227 8_1:-34866168 Msa1191100:five_prime_UTR
CCACAAATTCTAATCATCAT+CGG 0.577549 8_1:+34865721 None:intergenic
TTTCCGGATGTTCAGCAATG+AGG 0.587938 8_1:-34866188 Msa1191100:five_prime_UTR
GATATGAAGGTTGTATGAGG+AGG 0.588265 8_1:-34865484 Msa1191100:exon
AATGATATGAAGGTTGTATG+AGG 0.592701 8_1:-34865487 Msa1191100:exon
GGAAACCGTGACTCATGCCA+AGG 0.635067 8_1:+34865309 None:intergenic
CAAACGAGAAGAAAGGTGAA+CGG 0.643584 8_1:+34865596 None:intergenic
ATCCCACACAGCAATAGATG+TGG 0.664320 8_1:+34866084 None:intergenic
CAGCCACATCTATTGCTGTG+TGG 0.680974 8_1:-34866087 Msa1191100:CDS
AGTAAGCTCAGAAATTGTGG+AGG 0.681663 8_1:-34865676 Msa1191100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATAACTTCTTTTGTTTA+TGG - chr8_1:34865368-34865387 Msa1191100:three_prime_UTR 15.0%
!!! TTTTATTTTTTAATTTTGGG+TGG - chr8_1:34865650-34865669 Msa1191100:CDS 15.0%
!!! TTTATTTTTTAATTTTGGGT+GGG - chr8_1:34865651-34865670 Msa1191100:CDS 15.0%
!! GAAAATGTATGAATTAATTA+GGG + chr8_1:34866166-34866185 None:intergenic 15.0%
!! TGAAAATGTATGAATTAATT+AGG + chr8_1:34866167-34866186 None:intergenic 15.0%
!!! ATATAGATACTAACATTTTT+GGG + chr8_1:34866499-34866518 None:intergenic 15.0%
!!! AATATAGATACTAACATTTT+TGG + chr8_1:34866500-34866519 None:intergenic 15.0%
!!! TATTTTTTGTGAATTATATC+TGG - chr8_1:34866687-34866706 Msa1191100:intron 15.0%
!!! ATTTTTTGTGAATTATATCT+GGG - chr8_1:34866688-34866707 Msa1191100:intron 15.0%
!!! ATTCTTATATCAAACATTTT+TGG - chr8_1:34867142-34867161 Msa1191100:five_prime_UTR 15.0%
!!! AATATAACTTCTTTTGTTTA+TGG - chr8_1:34865368-34865387 Msa1191100:three_prime_UTR 15.0%
!!! TTTTATTTTTTAATTTTGGG+TGG - chr8_1:34865650-34865669 Msa1191100:CDS 15.0%
!!! TTTATTTTTTAATTTTGGGT+GGG - chr8_1:34865651-34865670 Msa1191100:CDS 15.0%
!! GAAAATGTATGAATTAATTA+GGG + chr8_1:34866166-34866185 None:intergenic 15.0%
!! TGAAAATGTATGAATTAATT+AGG + chr8_1:34866167-34866186 None:intergenic 15.0%
!!! ATATAGATACTAACATTTTT+GGG + chr8_1:34866499-34866518 None:intergenic 15.0%
!!! AATATAGATACTAACATTTT+TGG + chr8_1:34866500-34866519 None:intergenic 15.0%
!!! TATTTTTTGTGAATTATATC+TGG - chr8_1:34866687-34866706 Msa1191100:intron 15.0%
!!! ATTTTTTGTGAATTATATCT+GGG - chr8_1:34866688-34866707 Msa1191100:intron 15.0%
!!! ATTCTTATATCAAACATTTT+TGG - chr8_1:34867142-34867161 Msa1191100:five_prime_UTR 15.0%
!!! ATATGAACTTCTTTTGTTTA+TGG - chr8_1:34865328-34865347 Msa1191100:three_prime_UTR 20.0%
!!! TAGACAACTATTGTATATTT+TGG - chr8_1:34865577-34865596 Msa1191100:CDS 20.0%
!!! AGACAACTATTGTATATTTT+GGG - chr8_1:34865578-34865597 Msa1191100:CDS 20.0%
!!! TTATTTTTTAATTTTGGGTG+GGG - chr8_1:34865652-34865671 Msa1191100:CDS 20.0%
!! AAATGTTTACAATCCATTAT+AGG + chr8_1:34865744-34865763 None:intergenic 20.0%
!!! TATTTTACAAGTTTAAACCA+TGG + chr8_1:34865971-34865990 None:intergenic 20.0%
!! AAATGTTAGTATCTATATTC+CGG - chr8_1:34866501-34866520 Msa1191100:intron 20.0%
!! TGAAAAAACACAAATATATG+TGG - chr8_1:34866554-34866573 Msa1191100:intron 20.0%
!! GAAAAAACACAAATATATGT+GGG - chr8_1:34866555-34866574 Msa1191100:intron 20.0%
!! ATATTATAATGTAACCAGAA+AGG - chr8_1:34866655-34866674 Msa1191100:intron 20.0%
!! TTGATATAAGAATCTGTAAT+TGG + chr8_1:34867135-34867154 None:intergenic 20.0%
!!! ATATGAACTTCTTTTGTTTA+TGG - chr8_1:34865328-34865347 Msa1191100:three_prime_UTR 20.0%
!!! TAGACAACTATTGTATATTT+TGG - chr8_1:34865577-34865596 Msa1191100:CDS 20.0%
!!! AGACAACTATTGTATATTTT+GGG - chr8_1:34865578-34865597 Msa1191100:CDS 20.0%
!!! TTATTTTTTAATTTTGGGTG+GGG - chr8_1:34865652-34865671 Msa1191100:CDS 20.0%
!! AAATGTTTACAATCCATTAT+AGG + chr8_1:34865744-34865763 None:intergenic 20.0%
!!! TATTTTACAAGTTTAAACCA+TGG + chr8_1:34865971-34865990 None:intergenic 20.0%
!! AAATGTTAGTATCTATATTC+CGG - chr8_1:34866501-34866520 Msa1191100:intron 20.0%
!! TGAAAAAACACAAATATATG+TGG - chr8_1:34866554-34866573 Msa1191100:intron 20.0%
!! GAAAAAACACAAATATATGT+GGG - chr8_1:34866555-34866574 Msa1191100:intron 20.0%
!! ATATTATAATGTAACCAGAA+AGG - chr8_1:34866655-34866674 Msa1191100:intron 20.0%
!! TTGATATAAGAATCTGTAAT+TGG + chr8_1:34867135-34867154 None:intergenic 20.0%
! ACTATACTAATATATCTTGC+AGG - chr8_1:34865400-34865419 Msa1191100:three_prime_UTR 25.0%
!!! TATTTTTTAATTTTGGGTGG+GGG - chr8_1:34865653-34865672 Msa1191100:CDS 25.0%
!!! TTTTTTGCCATTTCCTATAA+TGG - chr8_1:34865728-34865747 Msa1191100:CDS 25.0%
! TTACAATCCATTATAGGAAA+TGG + chr8_1:34865738-34865757 None:intergenic 25.0%
! TTGATACATTACTCTGAATT+TGG - chr8_1:34865826-34865845 Msa1191100:intron 25.0%
! TGATACATTACTCTGAATTT+GGG - chr8_1:34865827-34865846 Msa1191100:intron 25.0%
! ATAACTAACAAACAACTTAC+GGG + chr8_1:34866045-34866064 None:intergenic 25.0%
! GATAACTAACAAACAACTTA+CGG + chr8_1:34866046-34866065 None:intergenic 25.0%
! TTGAAATGTGTAGATCAATT+TGG - chr8_1:34866099-34866118 Msa1191100:CDS 25.0%
! AATTAATTAGGGACATTATG+GGG + chr8_1:34866155-34866174 None:intergenic 25.0%
! GAATTAATTAGGGACATTAT+GGG + chr8_1:34866156-34866175 None:intergenic 25.0%
! TGAATTAATTAGGGACATTA+TGG + chr8_1:34866157-34866176 None:intergenic 25.0%
!!! GTGTTTTTTCATTTTTTCGA+GGG + chr8_1:34866545-34866564 None:intergenic 25.0%
!!! TGTGTTTTTTCATTTTTTCG+AGG + chr8_1:34866546-34866565 None:intergenic 25.0%
!! CAAATATATGTGGGTTAAAT+TGG - chr8_1:34866564-34866583 Msa1191100:intron 25.0%
! TAATCCAGAAAAGAAAATGT+TGG - chr8_1:34866735-34866754 Msa1191100:intron 25.0%
!! ATTTTCATCTCATTTGAAGA+GGG - chr8_1:34867006-34867025 Msa1191100:intron 25.0%
! ACTATACTAATATATCTTGC+AGG - chr8_1:34865400-34865419 Msa1191100:three_prime_UTR 25.0%
!!! TATTTTTTAATTTTGGGTGG+GGG - chr8_1:34865653-34865672 Msa1191100:CDS 25.0%
!!! TTTTTTGCCATTTCCTATAA+TGG - chr8_1:34865728-34865747 Msa1191100:CDS 25.0%
! TTACAATCCATTATAGGAAA+TGG + chr8_1:34865738-34865757 None:intergenic 25.0%
! TTGATACATTACTCTGAATT+TGG - chr8_1:34865826-34865845 Msa1191100:intron 25.0%
! TGATACATTACTCTGAATTT+GGG - chr8_1:34865827-34865846 Msa1191100:intron 25.0%
! ATAACTAACAAACAACTTAC+GGG + chr8_1:34866045-34866064 None:intergenic 25.0%
! GATAACTAACAAACAACTTA+CGG + chr8_1:34866046-34866065 None:intergenic 25.0%
! TTGAAATGTGTAGATCAATT+TGG - chr8_1:34866099-34866118 Msa1191100:CDS 25.0%
! AATTAATTAGGGACATTATG+GGG + chr8_1:34866155-34866174 None:intergenic 25.0%
! GAATTAATTAGGGACATTAT+GGG + chr8_1:34866156-34866175 None:intergenic 25.0%
! TGAATTAATTAGGGACATTA+TGG + chr8_1:34866157-34866176 None:intergenic 25.0%
!!! GTGTTTTTTCATTTTTTCGA+GGG + chr8_1:34866545-34866564 None:intergenic 25.0%
!!! TGTGTTTTTTCATTTTTTCG+AGG + chr8_1:34866546-34866565 None:intergenic 25.0%
!! CAAATATATGTGGGTTAAAT+TGG - chr8_1:34866564-34866583 Msa1191100:intron 25.0%
! TAATCCAGAAAAGAAAATGT+TGG - chr8_1:34866735-34866754 Msa1191100:intron 25.0%
!! ATTTTCATCTCATTTGAAGA+GGG - chr8_1:34867006-34867025 Msa1191100:intron 25.0%
! GAGTATTTTCTTCATGCTTA+TGG - chr8_1:34865453-34865472 Msa1191100:three_prime_UTR 30.0%
!!! TTTCCTTTTTCAGTAAACAC+TGG - chr8_1:34865542-34865561 Msa1191100:CDS 30.0%
!!! TTCCTTTTTCAGTAAACACT+GGG - chr8_1:34865543-34865562 Msa1191100:CDS 30.0%
!! TTGTATATTTTGGGAATTGC+AGG - chr8_1:34865587-34865606 Msa1191100:CDS 30.0%
TTTATATGTCTACTAGTGCT+AGG - chr8_1:34865761-34865780 Msa1191100:CDS 30.0%
TTATATGTCTACTAGTGCTA+GGG - chr8_1:34865762-34865781 Msa1191100:CDS 30.0%
ATATAGCTCCCAATCAATTT+GGG + chr8_1:34865885-34865904 None:intergenic 30.0%
AATATAGCTCCCAATCAATT+TGG + chr8_1:34865886-34865905 None:intergenic 30.0%
CAAAGGAAACAACATTGTAA+GGG + chr8_1:34865999-34866018 None:intergenic 30.0%
TCAAAGGAAACAACATTGTA+AGG + chr8_1:34866000-34866019 None:intergenic 30.0%
TCAATGGACATGATATTCAA+AGG + chr8_1:34866016-34866035 None:intergenic 30.0%
TTATCAATTTACCGATAGAC+TGG - chr8_1:34866061-34866080 Msa1191100:intron 30.0%
! TGTTTTCATAACCAGTCTAT+CGG + chr8_1:34866075-34866094 None:intergenic 30.0%
! TCAATTTGGAACTTATGAAC+TGG - chr8_1:34866113-34866132 Msa1191100:CDS 30.0%
GCAAATATCACATTCGATAT+GGG + chr8_1:34866304-34866323 None:intergenic 30.0%
TGCAAATATCACATTCGATA+TGG + chr8_1:34866305-34866324 None:intergenic 30.0%
! GAATTCTTTTGTACAAACTC+CGG + chr8_1:34866523-34866542 None:intergenic 30.0%
ATAATGTAACCAGAAAGGAA+TGG - chr8_1:34866660-34866679 Msa1191100:intron 30.0%
TAATGTAACCAGAAAGGAAT+GGG - chr8_1:34866661-34866680 Msa1191100:intron 30.0%
AAATATTTCCCATTCCTTTC+TGG + chr8_1:34866672-34866691 None:intergenic 30.0%
! TTTTCTTTTCTGGATTACAC+AGG + chr8_1:34866732-34866751 None:intergenic 30.0%
CCACAAATTCTAATCATCAT+CGG + chr8_1:34866806-34866825 None:intergenic 30.0%
CGATGATGATTAGAATTTGT+GGG - chr8_1:34866804-34866823 Msa1191100:intron 30.0%
AAAAGTAAGCTCAGAAATTG+TGG - chr8_1:34866845-34866864 Msa1191100:intron 30.0%
CTCTTCAAATGAGATGAAAA+TGG + chr8_1:34867007-34867026 None:intergenic 30.0%
! CATTTTCATCTCATTTGAAG+AGG - chr8_1:34867005-34867024 Msa1191100:intron 30.0%
! GGTAGTATTGAATGATATGA+AGG - chr8_1:34867027-34867046 Msa1191100:five_prime_UTR 30.0%
AATGATATGAAGGTTGTATG+AGG - chr8_1:34867037-34867056 Msa1191100:five_prime_UTR 30.0%
! GGCTTTTAGATAAAACGTAA+AGG - chr8_1:34867062-34867081 Msa1191100:five_prime_UTR 30.0%
! GAGTATTTTCTTCATGCTTA+TGG - chr8_1:34865453-34865472 Msa1191100:three_prime_UTR 30.0%
!!! TTTCCTTTTTCAGTAAACAC+TGG - chr8_1:34865542-34865561 Msa1191100:CDS 30.0%
!!! TTCCTTTTTCAGTAAACACT+GGG - chr8_1:34865543-34865562 Msa1191100:CDS 30.0%
!! TTGTATATTTTGGGAATTGC+AGG - chr8_1:34865587-34865606 Msa1191100:CDS 30.0%
TTTATATGTCTACTAGTGCT+AGG - chr8_1:34865761-34865780 Msa1191100:CDS 30.0%
TTATATGTCTACTAGTGCTA+GGG - chr8_1:34865762-34865781 Msa1191100:CDS 30.0%
ATATAGCTCCCAATCAATTT+GGG + chr8_1:34865885-34865904 None:intergenic 30.0%
AATATAGCTCCCAATCAATT+TGG + chr8_1:34865886-34865905 None:intergenic 30.0%
CAAAGGAAACAACATTGTAA+GGG + chr8_1:34865999-34866018 None:intergenic 30.0%
TCAAAGGAAACAACATTGTA+AGG + chr8_1:34866000-34866019 None:intergenic 30.0%
TCAATGGACATGATATTCAA+AGG + chr8_1:34866016-34866035 None:intergenic 30.0%
TTATCAATTTACCGATAGAC+TGG - chr8_1:34866061-34866080 Msa1191100:intron 30.0%
! TGTTTTCATAACCAGTCTAT+CGG + chr8_1:34866075-34866094 None:intergenic 30.0%
! TCAATTTGGAACTTATGAAC+TGG - chr8_1:34866113-34866132 Msa1191100:CDS 30.0%
GCAAATATCACATTCGATAT+GGG + chr8_1:34866304-34866323 None:intergenic 30.0%
TGCAAATATCACATTCGATA+TGG + chr8_1:34866305-34866324 None:intergenic 30.0%
! GAATTCTTTTGTACAAACTC+CGG + chr8_1:34866523-34866542 None:intergenic 30.0%
ATAATGTAACCAGAAAGGAA+TGG - chr8_1:34866660-34866679 Msa1191100:intron 30.0%
TAATGTAACCAGAAAGGAAT+GGG - chr8_1:34866661-34866680 Msa1191100:intron 30.0%
AAATATTTCCCATTCCTTTC+TGG + chr8_1:34866672-34866691 None:intergenic 30.0%
! TTTTCTTTTCTGGATTACAC+AGG + chr8_1:34866732-34866751 None:intergenic 30.0%
CCACAAATTCTAATCATCAT+CGG + chr8_1:34866806-34866825 None:intergenic 30.0%
CGATGATGATTAGAATTTGT+GGG - chr8_1:34866804-34866823 Msa1191100:intron 30.0%
AAAAGTAAGCTCAGAAATTG+TGG - chr8_1:34866845-34866864 Msa1191100:intron 30.0%
CTCTTCAAATGAGATGAAAA+TGG + chr8_1:34867007-34867026 None:intergenic 30.0%
! CATTTTCATCTCATTTGAAG+AGG - chr8_1:34867005-34867024 Msa1191100:intron 30.0%
! GGTAGTATTGAATGATATGA+AGG - chr8_1:34867027-34867046 Msa1191100:five_prime_UTR 30.0%
AATGATATGAAGGTTGTATG+AGG - chr8_1:34867037-34867056 Msa1191100:five_prime_UTR 30.0%
! GGCTTTTAGATAAAACGTAA+AGG - chr8_1:34867062-34867081 Msa1191100:five_prime_UTR 30.0%
ATACTCCTTTCCAAGCAAAT+GGG + chr8_1:34865439-34865458 None:intergenic 35.0%
AATACTCCTTTCCAAGCAAA+TGG + chr8_1:34865440-34865459 None:intergenic 35.0%
! TCAGCAGAAAGAGCATATAT+AGG + chr8_1:34865480-34865499 None:intergenic 35.0%
!!! TCCTTTTTCAGTAAACACTG+GGG - chr8_1:34865544-34865563 Msa1191100:CDS 35.0%
GAAACAACTCATTACTCAAC+AGG + chr8_1:34865858-34865877 None:intergenic 35.0%
! TTGAGTAATGAGTTGTTTCC+TGG - chr8_1:34865859-34865878 Msa1191100:intron 35.0%
ACTTCCATAACATTGTTTCG+TGG - chr8_1:34865917-34865936 Msa1191100:intron 35.0%
AACTCCACGAAACAATGTTA+TGG + chr8_1:34865924-34865943 None:intergenic 35.0%
! AACTTACGGGATTGATTCAA+TGG + chr8_1:34866032-34866051 None:intergenic 35.0%
TCATCCTTTCATCCATGAAA+AGG - chr8_1:34866219-34866238 Msa1191100:intron 35.0%
CATCCTTTCATCCATGAAAA+GGG - chr8_1:34866220-34866239 Msa1191100:intron 35.0%
! GTAATCTTCATCCCTTTTCA+TGG + chr8_1:34866234-34866253 None:intergenic 35.0%
TATCACATTCGATATGGGAA+GGG + chr8_1:34866299-34866318 None:intergenic 35.0%
ATATCACATTCGATATGGGA+AGG + chr8_1:34866300-34866319 None:intergenic 35.0%
AGCAATGAGGATGTTACTAT+TGG - chr8_1:34866349-34866368 Msa1191100:intron 35.0%
AAAAACGAACCTGAACACTT+AGG + chr8_1:34866469-34866488 None:intergenic 35.0%
! AGCTCCAACATTTTCTTTTC+TGG + chr8_1:34866742-34866761 None:intergenic 35.0%
CCGATGATGATTAGAATTTG+TGG - chr8_1:34866803-34866822 Msa1191100:intron 35.0%
ATGATTAGAATTTGTGGGAG+TGG - chr8_1:34866809-34866828 Msa1191100:intron 35.0%
TGATTAGAATTTGTGGGAGT+GGG - chr8_1:34866810-34866829 Msa1191100:intron 35.0%
CTATCTTCAAACGAGAAGAA+AGG + chr8_1:34866938-34866957 None:intergenic 35.0%
CTTTCTTCTCGTTTGAAGAT+AGG - chr8_1:34866936-34866955 Msa1191100:intron 35.0%
ATATGAAGGTTGTATGAGGA+GGG - chr8_1:34867041-34867060 Msa1191100:five_prime_UTR 35.0%
AAAAATGTGTGTTCACTCCT+TGG - chr8_1:34867198-34867217 Msa1191100:five_prime_UTR 35.0%
ATACTCCTTTCCAAGCAAAT+GGG + chr8_1:34865439-34865458 None:intergenic 35.0%
AATACTCCTTTCCAAGCAAA+TGG + chr8_1:34865440-34865459 None:intergenic 35.0%
! TCAGCAGAAAGAGCATATAT+AGG + chr8_1:34865480-34865499 None:intergenic 35.0%
!!! TCCTTTTTCAGTAAACACTG+GGG - chr8_1:34865544-34865563 Msa1191100:CDS 35.0%
GAAACAACTCATTACTCAAC+AGG + chr8_1:34865858-34865877 None:intergenic 35.0%
! TTGAGTAATGAGTTGTTTCC+TGG - chr8_1:34865859-34865878 Msa1191100:intron 35.0%
ACTTCCATAACATTGTTTCG+TGG - chr8_1:34865917-34865936 Msa1191100:intron 35.0%
AACTCCACGAAACAATGTTA+TGG + chr8_1:34865924-34865943 None:intergenic 35.0%
! AACTTACGGGATTGATTCAA+TGG + chr8_1:34866032-34866051 None:intergenic 35.0%
TCATCCTTTCATCCATGAAA+AGG - chr8_1:34866219-34866238 Msa1191100:intron 35.0%
CATCCTTTCATCCATGAAAA+GGG - chr8_1:34866220-34866239 Msa1191100:intron 35.0%
! GTAATCTTCATCCCTTTTCA+TGG + chr8_1:34866234-34866253 None:intergenic 35.0%
TATCACATTCGATATGGGAA+GGG + chr8_1:34866299-34866318 None:intergenic 35.0%
ATATCACATTCGATATGGGA+AGG + chr8_1:34866300-34866319 None:intergenic 35.0%
AGCAATGAGGATGTTACTAT+TGG - chr8_1:34866349-34866368 Msa1191100:intron 35.0%
AAAAACGAACCTGAACACTT+AGG + chr8_1:34866469-34866488 None:intergenic 35.0%
! AGCTCCAACATTTTCTTTTC+TGG + chr8_1:34866742-34866761 None:intergenic 35.0%
CCGATGATGATTAGAATTTG+TGG - chr8_1:34866803-34866822 Msa1191100:intron 35.0%
ATGATTAGAATTTGTGGGAG+TGG - chr8_1:34866809-34866828 Msa1191100:intron 35.0%
TGATTAGAATTTGTGGGAGT+GGG - chr8_1:34866810-34866829 Msa1191100:intron 35.0%
CTATCTTCAAACGAGAAGAA+AGG + chr8_1:34866938-34866957 None:intergenic 35.0%
CTTTCTTCTCGTTTGAAGAT+AGG - chr8_1:34866936-34866955 Msa1191100:intron 35.0%
ATATGAAGGTTGTATGAGGA+GGG - chr8_1:34867041-34867060 Msa1191100:five_prime_UTR 35.0%
AAAAATGTGTGTTCACTCCT+TGG - chr8_1:34867198-34867217 Msa1191100:five_prime_UTR 35.0%
TTTCCAAGCAAATGGGATAG+TGG + chr8_1:34865432-34865451 None:intergenic 40.0%
ACTATCCCATTTGCTTGGAA+AGG - chr8_1:34865431-34865450 Msa1191100:three_prime_UTR 40.0%
!! TGCTGATGTTGTTTCAAGCT+TGG - chr8_1:34865494-34865513 Msa1191100:CDS 40.0%
TGGTTGTTCTTCGAAATGAC+AGG - chr8_1:34865514-34865533 Msa1191100:CDS 40.0%
ACCCCAGTGTTTACTGAAAA+AGG + chr8_1:34865548-34865567 None:intergenic 40.0%
!!! TTTTTAATTTTGGGTGGGGG+TGG - chr8_1:34865656-34865675 Msa1191100:CDS 40.0%
!!! TTTTAATTTTGGGTGGGGGT+GGG - chr8_1:34865657-34865676 Msa1191100:CDS 40.0%
TTTCCTGGCCCAAATTGATT+GGG - chr8_1:34865874-34865893 Msa1191100:intron 40.0%
!! TCATTTGGTTCTTGAGTCCA+TGG - chr8_1:34865951-34865970 Msa1191100:intron 40.0%
! CATCCCTTTTCATGGATGAA+AGG + chr8_1:34866226-34866245 None:intergenic 40.0%
ATCACATTCGATATGGGAAG+GGG + chr8_1:34866298-34866317 None:intergenic 40.0%
TTGCATTGTGTCACAGTTTC+CGG - chr8_1:34866320-34866339 Msa1191100:intron 40.0%
AGGATGTTACTATTGGAGCT+TGG - chr8_1:34866356-34866375 Msa1191100:intron 40.0%
TGGGATATTCCTAAGTGTTC+AGG - chr8_1:34866457-34866476 Msa1191100:intron 40.0%
GGTTAAATTGGACTTCCTTG+TGG - chr8_1:34866576-34866595 Msa1191100:intron 40.0%
TTGTGGTATTGTTCACCACA+AGG + chr8_1:34866594-34866613 None:intergenic 40.0%
TTGTGGTGAACAATACCACA+AGG - chr8_1:34866593-34866612 Msa1191100:intron 40.0%
AATGTGTGCATACACTCGAA+TGG + chr8_1:34866623-34866642 None:intergenic 40.0%
AATGTTGGAGCTCCATCAAA+AGG - chr8_1:34866750-34866769 Msa1191100:intron 40.0%
TGTGGGAGTGGGATATTATT+TGG - chr8_1:34866821-34866840 Msa1191100:intron 40.0%
GTGGGAGTGGGATATTATTT+GGG - chr8_1:34866822-34866841 Msa1191100:intron 40.0%
AGTAAGCTCAGAAATTGTGG+AGG - chr8_1:34866848-34866867 Msa1191100:intron 40.0%
AAACGAGAAGAAAGGTGAAC+GGG + chr8_1:34866930-34866949 None:intergenic 40.0%
CAAACGAGAAGAAAGGTGAA+CGG + chr8_1:34866931-34866950 None:intergenic 40.0%
GATATGAAGGTTGTATGAGG+AGG - chr8_1:34867040-34867059 Msa1191100:five_prime_UTR 40.0%
TTTCCAAGCAAATGGGATAG+TGG + chr8_1:34865432-34865451 None:intergenic 40.0%
ACTATCCCATTTGCTTGGAA+AGG - chr8_1:34865431-34865450 Msa1191100:three_prime_UTR 40.0%
!! TGCTGATGTTGTTTCAAGCT+TGG - chr8_1:34865494-34865513 Msa1191100:CDS 40.0%
TGGTTGTTCTTCGAAATGAC+AGG - chr8_1:34865514-34865533 Msa1191100:CDS 40.0%
ACCCCAGTGTTTACTGAAAA+AGG + chr8_1:34865548-34865567 None:intergenic 40.0%
!!! TTTTTAATTTTGGGTGGGGG+TGG - chr8_1:34865656-34865675 Msa1191100:CDS 40.0%
!!! TTTTAATTTTGGGTGGGGGT+GGG - chr8_1:34865657-34865676 Msa1191100:CDS 40.0%
TTTCCTGGCCCAAATTGATT+GGG - chr8_1:34865874-34865893 Msa1191100:intron 40.0%
!! TCATTTGGTTCTTGAGTCCA+TGG - chr8_1:34865951-34865970 Msa1191100:intron 40.0%
! CATCCCTTTTCATGGATGAA+AGG + chr8_1:34866226-34866245 None:intergenic 40.0%
ATCACATTCGATATGGGAAG+GGG + chr8_1:34866298-34866317 None:intergenic 40.0%
TTGCATTGTGTCACAGTTTC+CGG - chr8_1:34866320-34866339 Msa1191100:intron 40.0%
AGGATGTTACTATTGGAGCT+TGG - chr8_1:34866356-34866375 Msa1191100:intron 40.0%
TGGGATATTCCTAAGTGTTC+AGG - chr8_1:34866457-34866476 Msa1191100:intron 40.0%
GGTTAAATTGGACTTCCTTG+TGG - chr8_1:34866576-34866595 Msa1191100:intron 40.0%
TTGTGGTATTGTTCACCACA+AGG + chr8_1:34866594-34866613 None:intergenic 40.0%
TTGTGGTGAACAATACCACA+AGG - chr8_1:34866593-34866612 Msa1191100:intron 40.0%
AATGTGTGCATACACTCGAA+TGG + chr8_1:34866623-34866642 None:intergenic 40.0%
AATGTTGGAGCTCCATCAAA+AGG - chr8_1:34866750-34866769 Msa1191100:intron 40.0%
TGTGGGAGTGGGATATTATT+TGG - chr8_1:34866821-34866840 Msa1191100:intron 40.0%
GTGGGAGTGGGATATTATTT+GGG - chr8_1:34866822-34866841 Msa1191100:intron 40.0%
AGTAAGCTCAGAAATTGTGG+AGG - chr8_1:34866848-34866867 Msa1191100:intron 40.0%
AAACGAGAAGAAAGGTGAAC+GGG + chr8_1:34866930-34866949 None:intergenic 40.0%
CAAACGAGAAGAAAGGTGAA+CGG + chr8_1:34866931-34866950 None:intergenic 40.0%
GATATGAAGGTTGTATGAGG+AGG - chr8_1:34867040-34867059 Msa1191100:five_prime_UTR 40.0%
!! TTTAATTTTGGGTGGGGGTG+GGG - chr8_1:34865658-34865677 Msa1191100:CDS 45.0%
ATGTCTACTAGTGCTAGGGT+TGG - chr8_1:34865766-34865785 Msa1191100:CDS 45.0%
GTTTCCTGGCCCAAATTGAT+TGG - chr8_1:34865873-34865892 Msa1191100:intron 45.0%
GTGGAGTTTGTCCACTCATT+TGG - chr8_1:34865936-34865955 Msa1191100:intron 45.0%
GGACTCAAGAACCAAATGAG+TGG + chr8_1:34865950-34865969 None:intergenic 45.0%
TTTCCGGATGTTCAGCAATG+AGG - chr8_1:34866336-34866355 Msa1191100:intron 45.0%
CATCCTCATTGCTGAACATC+CGG + chr8_1:34866342-34866361 None:intergenic 45.0%
AGCCACATCTATTGCTGTGT+GGG - chr8_1:34866438-34866457 Msa1191100:intron 45.0%
ATCCCACACAGCAATAGATG+TGG + chr8_1:34866443-34866462 None:intergenic 45.0%
CTGGGTTATTGTATACCTGC+AGG - chr8_1:34866706-34866725 Msa1191100:intron 45.0%
!! ATGGCTTTGGTAGCATCTTC+TGG + chr8_1:34866902-34866921 None:intergenic 45.0%
TGAACGGGAAAGAATGGCTT+TGG + chr8_1:34866915-34866934 None:intergenic 45.0%
GAAAGGTGAACGGGAAAGAA+TGG + chr8_1:34866921-34866940 None:intergenic 45.0%
!! TTTAATTTTGGGTGGGGGTG+GGG - chr8_1:34865658-34865677 Msa1191100:CDS 45.0%
ATGTCTACTAGTGCTAGGGT+TGG - chr8_1:34865766-34865785 Msa1191100:CDS 45.0%
GTTTCCTGGCCCAAATTGAT+TGG - chr8_1:34865873-34865892 Msa1191100:intron 45.0%
GTGGAGTTTGTCCACTCATT+TGG - chr8_1:34865936-34865955 Msa1191100:intron 45.0%
GGACTCAAGAACCAAATGAG+TGG + chr8_1:34865950-34865969 None:intergenic 45.0%
TTTCCGGATGTTCAGCAATG+AGG - chr8_1:34866336-34866355 Msa1191100:intron 45.0%
CATCCTCATTGCTGAACATC+CGG + chr8_1:34866342-34866361 None:intergenic 45.0%
AGCCACATCTATTGCTGTGT+GGG - chr8_1:34866438-34866457 Msa1191100:intron 45.0%
ATCCCACACAGCAATAGATG+TGG + chr8_1:34866443-34866462 None:intergenic 45.0%
CTGGGTTATTGTATACCTGC+AGG - chr8_1:34866706-34866725 Msa1191100:intron 45.0%
!! ATGGCTTTGGTAGCATCTTC+TGG + chr8_1:34866902-34866921 None:intergenic 45.0%
TGAACGGGAAAGAATGGCTT+TGG + chr8_1:34866915-34866934 None:intergenic 45.0%
GAAAGGTGAACGGGAAAGAA+TGG + chr8_1:34866921-34866940 None:intergenic 45.0%
!!! TGTTTTTTATTTTTTAATTT+TGG - chr8_1:34865646-34865665 Msa1191100:CDS 5.0%
!!! GTTTTTTATTTTTTAATTTT+GGG - chr8_1:34865647-34865666 Msa1191100:CDS 5.0%
!!! TGTTTTTTATTTTTTAATTT+TGG - chr8_1:34865646-34865665 Msa1191100:CDS 5.0%
!!! GTTTTTTATTTTTTAATTTT+GGG - chr8_1:34865647-34865666 Msa1191100:CDS 5.0%
GAGCCACTATCCCATTTGCT+TGG - chr8_1:34865426-34865445 Msa1191100:three_prime_UTR 50.0%
!! TTAATTTTGGGTGGGGGTGG+GGG - chr8_1:34865659-34865678 Msa1191100:CDS 50.0%
ATTCGATATGGGAAGGGGTC+AGG + chr8_1:34866293-34866312 None:intergenic 50.0%
CAGCCACATCTATTGCTGTG+TGG - chr8_1:34866437-34866456 Msa1191100:intron 50.0%
! CACTCGAATGGACTTCCTTG+TGG + chr8_1:34866611-34866630 None:intergenic 50.0%
! GAGAGCAGCTCTCCTTTTGA+TGG + chr8_1:34866765-34866784 None:intergenic 50.0%
CATCATCGGATTCCAGCGTT+GGG + chr8_1:34866792-34866811 None:intergenic 50.0%
TCATCATCGGATTCCAGCGT+TGG + chr8_1:34866793-34866812 None:intergenic 50.0%
TCACTCCTTGGCATGAGTCA+CGG - chr8_1:34867210-34867229 Msa1191100:five_prime_UTR 50.0%
GAGCCACTATCCCATTTGCT+TGG - chr8_1:34865426-34865445 Msa1191100:three_prime_UTR 50.0%
!! TTAATTTTGGGTGGGGGTGG+GGG - chr8_1:34865659-34865678 Msa1191100:CDS 50.0%
ATTCGATATGGGAAGGGGTC+AGG + chr8_1:34866293-34866312 None:intergenic 50.0%
CAGCCACATCTATTGCTGTG+TGG - chr8_1:34866437-34866456 Msa1191100:intron 50.0%
! CACTCGAATGGACTTCCTTG+TGG + chr8_1:34866611-34866630 None:intergenic 50.0%
! GAGAGCAGCTCTCCTTTTGA+TGG + chr8_1:34866765-34866784 None:intergenic 50.0%
CATCATCGGATTCCAGCGTT+GGG + chr8_1:34866792-34866811 None:intergenic 50.0%
TCATCATCGGATTCCAGCGT+TGG + chr8_1:34866793-34866812 None:intergenic 50.0%
TCACTCCTTGGCATGAGTCA+CGG - chr8_1:34867210-34867229 Msa1191100:five_prime_UTR 50.0%
!! TAATTTTGGGTGGGGGTGGG+GGG - chr8_1:34865660-34865679 Msa1191100:CDS 55.0%
GCTCCCAATCAATTTGGGCC+AGG + chr8_1:34865880-34865899 None:intergenic 55.0%
TCTGGATTACACAGGCCTGC+AGG + chr8_1:34866724-34866743 None:intergenic 55.0%
GGAAACCGTGACTCATGCCA+AGG + chr8_1:34867218-34867237 None:intergenic 55.0%
!! TAATTTTGGGTGGGGGTGGG+GGG - chr8_1:34865660-34865679 Msa1191100:CDS 55.0%
GCTCCCAATCAATTTGGGCC+AGG + chr8_1:34865880-34865899 None:intergenic 55.0%
TCTGGATTACACAGGCCTGC+AGG + chr8_1:34866724-34866743 None:intergenic 55.0%
GGAAACCGTGACTCATGCCA+AGG + chr8_1:34867218-34867237 None:intergenic 55.0%
CTGCTCTCAGAGCCCAACGC+TGG - chr8_1:34866777-34866796 Msa1191100:intron 65.0%
CTGCTCTCAGAGCCCAACGC+TGG - chr8_1:34866777-34866796 Msa1191100:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 34865306 34867240 34865306 ID=Msa1191100;Name=Msa1191100
chr8_1 mRNA 34865306 34867240 34865306 ID=Msa1191100-mRNA-1;Parent=Msa1191100;Name=Msa1191100-mRNA-1;_AED=0.00;_eAED=0.00;_QI=286|1|1|1|1|1|3|183|138
chr8_1 exon 34865306 34865818 34865306 ID=Msa1191100-mRNA-1:exon:11598;Parent=Msa1191100-mRNA-1
chr8_1 exon 34866068 34866210 34866068 ID=Msa1191100-mRNA-1:exon:11597;Parent=Msa1191100-mRNA-1
chr8_1 exon 34867011 34867240 34867011 ID=Msa1191100-mRNA-1:exon:11596;Parent=Msa1191100-mRNA-1
chr8_1 five_prime_UTR 34867011 34867240 34867011 ID=Msa1191100-mRNA-1:five_prime_utr;Parent=Msa1191100-mRNA-1
chr8_1 five_prime_UTR 34866155 34866210 34866155 ID=Msa1191100-mRNA-1:five_prime_utr;Parent=Msa1191100-mRNA-1
chr8_1 CDS 34866068 34866154 34866068 ID=Msa1191100-mRNA-1:cds;Parent=Msa1191100-mRNA-1
chr8_1 CDS 34865489 34865818 34865489 ID=Msa1191100-mRNA-1:cds;Parent=Msa1191100-mRNA-1
chr8_1 three_prime_UTR 34865306 34865488 34865306 ID=Msa1191100-mRNA-1:three_prime_utr;Parent=Msa1191100-mRNA-1
Gene Sequence

>Msa1191100

ATGTCAAACATGAGAACAATCTTGAACTTTGTGCTTCAGATTGTACAGCCACATCTATTGCTGTGTGGGATATTCCTAAGTGTTCAGGCCTGTGTAATCCAGAAAAGAAAATGTTGGAGCTCCATCAAAAGGAGAGCTGCTCTCAGAGCCCAACGCTGGAATCCGATGATGATTAGAATTTGTGGGAGTGGGATATTATTTGGGAAAAGTAAGCTCAGAAATTGTGGAGGAAGTTCTTCTTGTTGTCATTTCTTAATGCCAGAAGATGCTACCAAAGCCATTCTTTCCCGTTCACCTTTCTTCTCGTTTGAAGATAGGTTGTTGAGTTTTCTTTCATATCTCTTTCACACACACACACACTCTCCATTTTCATCTCATTTGAAGAGGGTAGTATTGAATGATATGAAGGTTGTATGA
Protein sequence

>Msa1191100

MSNMRTILNFVLQIVQPHLLLCGIFLSVQACVIQKRKCWSSIKRRAALRAQRWNPMMIRICGSGILFGKSKLRNCGGSSSCCHFLMPEDATKAILSRSPFFSFEDRLLSFLSYLFHTHTHSPFSSHLKRVVLNDMKVV*