AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0265200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0265200 MtrunA17_Chr3g0121631 91.935 124 8 1 1 124 1 122 1.79e-74 218
Msa0265200 MtrunA17_Chr4g0061451 52.893 121 52 3 1 116 1 121 2.14e-36 125
Msa0265200 MtrunA17_Chr7g0219021 48.031 127 59 3 1 124 1 123 1.05e-34 120
Msa0265200 MtrunA17_Chr3g0086861 43.511 131 63 3 1 124 1 127 1.30e-30 113
Msa0265200 MtrunA17_Chr7g0219031 46.729 107 54 2 1 104 1 107 5.81e-28 99.8
Msa0265200 MtrunA17_Chr3g0119311 50.588 85 41 1 38 121 36 120 4.11e-26 95.5
Msa0265200 MtrunA17_Chr7g0266751 46.535 101 50 2 25 121 19 119 1.15e-24 92.0
Msa0265200 MtrunA17_Chr4g0035001 51.316 76 36 1 39 113 7 82 1.87e-23 89.4
Msa0265200 MtrunA17_Chr4g0035051 48.684 76 38 1 39 113 9 84 1.23e-22 85.9
Msa0265200 MtrunA17_Chr4g0031091 43.820 89 49 1 28 115 23 111 2.12e-22 85.1
Msa0265200 MtrunA17_Chr4g0035031 50.000 76 37 1 39 113 9 84 9.66e-22 85.9
Msa0265200 MtrunA17_Chr2g0278661 50.649 77 37 1 39 114 8 84 8.64e-20 81.6
Msa0265200 MtrunA17_Chr3g0116631 44.318 88 44 2 39 125 207 290 2.06e-19 81.6
Msa0265200 MtrunA17_Chr3g0116631 39.362 94 53 2 31 123 102 192 2.36e-11 59.7
Msa0265200 MtrunA17_Chr7g0263001 39.669 121 63 3 1 117 1 115 5.42e-19 77.4
Msa0265200 MtrunA17_Chr1g0181111 49.367 79 39 1 39 116 197 275 1.11e-18 79.3
Msa0265200 MtrunA17_Chr4g0042161 43.137 102 51 3 33 131 44 141 1.90e-18 75.9
Msa0265200 MtrunA17_Chr1g0198431 37.895 95 55 2 35 128 189 280 1.66e-17 76.3
Msa0265200 MtrunA17_Chr3g0101061 44.565 92 47 3 29 117 28 118 4.64e-17 76.3
Msa0265200 MtrunA17_Chr4g0058151 44.304 79 43 1 39 116 217 295 7.32e-17 74.7
Msa0265200 MtrunA17_Chr4g0058151 43.529 85 45 2 39 122 123 205 2.34e-12 62.4
Msa0265200 MtrunA17_Chr1g0168601 45.679 81 43 1 39 118 154 234 1.36e-16 73.2
Msa0265200 MtrunA17_Chr4g0050781 41.250 80 46 1 39 117 8 87 1.65e-16 72.4
Msa0265200 MtrunA17_Chr4g0038111 38.235 102 62 1 32 133 333 433 9.96e-16 72.4
Msa0265200 MtrunA17_Chr6g0449481 35.870 92 58 1 33 123 128 219 2.10e-15 71.2
Msa0265200 MtrunA17_Chr1g0210241 37.647 85 52 1 36 119 5 89 2.39e-15 70.5
Msa0265200 MtrunA17_Chr3g0110211 42.697 89 49 2 38 124 112 200 5.83e-15 70.1
Msa0265200 MtrunA17_Chr3g0144311 34.884 86 56 0 36 121 27 112 9.78e-15 68.9
Msa0265200 MtrunA17_Chr1g0186351 36.471 85 54 0 34 118 108 192 1.29e-14 69.3
Msa0265200 MtrunA17_Chr1g0186351 36.709 79 49 1 39 117 7 84 7.44e-12 61.2
Msa0265200 MtrunA17_Chr1g0201001 37.975 79 47 2 40 116 31 109 1.38e-14 69.3
Msa0265200 MtrunA17_Chr4g0017401 35.484 93 56 1 38 126 16 108 3.20e-14 67.4
Msa0265200 MtrunA17_Chr5g0447761 41.250 80 46 1 37 116 5 83 3.28e-14 68.2
Msa0265200 MtrunA17_Chr6g0481071 35.000 80 51 1 38 116 95 174 3.48e-14 65.9
Msa0265200 MtrunA17_Chr7g0276531 40.580 69 41 0 38 106 18 86 3.48e-14 67.0
Msa0265200 MtrunA17_Chr4g0070231 45.333 75 40 1 40 113 133 207 3.91e-14 67.8
Msa0265200 MtrunA17_Chr3g0088511 35.556 90 54 3 24 110 17 105 4.07e-14 64.7
Msa0265200 MtrunA17_Chr8g0344491 45.333 75 40 1 40 113 149 223 5.66e-14 67.4
Msa0265200 MtrunA17_Chr8g0346011 42.029 69 40 0 38 106 37 105 6.74e-14 66.6
Msa0265200 MtrunA17_Chr7g0267571 40.230 87 51 1 36 121 210 296 1.07e-13 66.2
Msa0265200 MtrunA17_Chr1g0163591 41.176 85 49 1 37 121 161 244 2.40e-13 65.9
Msa0265200 MtrunA17_Chr5g0430471 33.673 98 56 2 23 111 16 113 2.50e-13 62.8
Msa0265200 MtrunA17_Chr2g0290191 40.244 82 48 1 35 116 1 81 2.63e-13 65.5
Msa0265200 MtrunA17_Chr2g0290191 27.869 122 79 2 9 130 85 197 1.09e-11 60.8
Msa0265200 MtrunA17_Chr4g0042151 32.479 117 73 3 2 116 11 123 3.19e-13 63.2
Msa0265200 MtrunA17_Chr3g0135111 34.118 85 56 0 33 117 112 196 3.48e-13 65.1
Msa0265200 MtrunA17_Chr3g0135111 38.636 88 53 1 29 116 1 87 6.74e-12 61.2
Msa0265200 MtrunA17_Chr1g0153181 37.000 100 59 2 19 114 10 109 3.73e-13 65.1
Msa0265200 MtrunA17_Chr2g0327921 36.250 80 50 1 38 116 7 86 5.44e-13 60.5
Msa0265200 MtrunA17_Chr3g0127751 40.260 77 44 1 38 112 26 102 6.39e-13 63.5
Msa0265200 MtrunA17_Chr5g0426311 35.065 77 50 0 38 114 16 92 8.70e-13 63.5
Msa0265200 MtrunA17_Chr7g0268551 38.824 85 51 1 38 121 348 432 1.07e-12 63.5
Msa0265200 MtrunA17_Chr4g0065741 37.500 80 49 1 36 114 69 148 1.44e-12 61.2
Msa0265200 MtrunA17_Chr5g0419351 36.250 80 50 1 35 113 7 86 1.75e-12 61.2
Msa0265200 MtrunA17_Chr8g0360551 43.056 72 41 0 35 106 70 141 1.94e-12 63.2
Msa0265200 MtrunA17_Chr3g0139111 42.667 75 42 1 40 113 147 221 2.17e-12 62.8
Msa0265200 MtrunA17_Chr8g0375101 36.145 83 52 1 35 117 1 82 8.86e-12 60.8
Msa0265200 MtrunA17_Chr1g0153871 36.364 88 54 2 40 125 192 279 1.27e-11 60.5
Msa0265200 MtrunA17_Chr1g0177101 37.349 83 51 1 36 117 205 287 1.53e-11 60.1
Msa0265200 MtrunA17_Chr4g0018581 39.024 82 49 1 33 113 446 527 1.70e-11 60.5
Msa0265200 MtrunA17_Chr4g0044541 37.975 79 48 1 37 114 33 111 3.19e-11 59.7
Msa0265200 MtrunA17_Chr4g0045921 30.769 91 61 1 35 125 160 248 3.28e-11 59.3
Msa0265200 MtrunA17_Chr4g0045921 39.189 74 45 0 33 106 71 144 6.81e-11 58.5
Msa0265200 MtrunA17_Chr2g0328791 39.759 83 48 2 40 120 110 192 5.09e-11 58.9
Msa0265200 MtrunA17_Chr5g0402191 36.145 83 52 1 35 117 1 82 8.24e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0265200 AT3G23830.2 59.350 123 45 4 1 122 1 119 5.21e-44 141
Msa0265200 AT3G23830.1 59.350 123 45 4 1 122 1 119 5.21e-44 141
Msa0265200 AT4G13850.3 60.976 123 43 4 1 122 1 119 8.38e-43 138
Msa0265200 AT4G13850.4 62.393 117 40 3 1 116 1 114 9.91e-43 137
Msa0265200 AT4G13850.2 60.976 123 43 4 1 122 1 119 1.10e-42 138
Msa0265200 AT4G13850.1 60.976 123 43 4 1 122 1 119 1.28e-42 138
Msa0265200 AT5G61030.2 55.462 119 50 2 1 116 1 119 5.72e-40 136
Msa0265200 AT5G61030.1 55.462 119 50 2 1 116 1 119 5.72e-40 136
Msa0265200 AT1G74230.1 53.043 115 50 1 1 115 1 111 3.96e-37 128
Msa0265200 AT5G47320.1 55.172 116 44 3 1 115 1 109 2.29e-33 116
Msa0265200 AT3G08000.1 58.333 84 34 1 39 121 42 125 2.57e-27 99.0
Msa0265200 AT5G06210.1 50.000 96 45 2 33 127 29 122 2.75e-23 89.0
Msa0265200 AT2G21660.2 48.684 76 38 1 39 113 9 84 2.61e-21 84.3
Msa0265200 AT5G59865.1 47.191 89 46 1 36 123 24 112 3.77e-21 82.4
Msa0265200 AT2G21660.1 48.684 76 38 1 39 113 9 84 3.88e-21 84.3
Msa0265200 AT2G37220.1 53.165 79 36 1 39 116 205 283 6.56e-21 85.9
Msa0265200 AT2G37220.1 41.860 86 43 2 39 123 92 171 4.18e-12 62.0
Msa0265200 AT3G53460.1 50.602 83 40 1 39 120 258 340 2.25e-20 85.1
Msa0265200 AT3G53460.1 43.023 86 42 2 39 123 100 179 7.55e-11 58.5
Msa0265200 AT3G53460.3 50.602 83 40 1 39 120 258 340 2.25e-20 85.1
Msa0265200 AT3G53460.3 43.023 86 42 2 39 123 100 179 7.55e-11 58.5
Msa0265200 AT3G53460.2 50.602 83 40 1 39 120 258 340 2.25e-20 85.1
Msa0265200 AT3G53460.2 43.023 86 42 2 39 123 100 179 7.55e-11 58.5
Msa0265200 AT3G53460.4 50.602 83 40 1 39 120 279 361 2.86e-20 85.1
Msa0265200 AT4G39260.3 38.776 98 47 2 39 135 7 92 6.08e-20 78.6
Msa0265200 AT1G18630.1 40.000 115 63 3 1 114 4 113 3.16e-19 78.6
Msa0265200 AT4G24770.1 47.126 87 42 2 39 124 245 328 1.30e-18 80.1
Msa0265200 AT4G24770.1 39.000 100 57 2 25 123 137 233 3.61e-12 62.4
Msa0265200 AT4G24770.2 47.126 87 42 2 39 124 245 328 1.30e-18 80.1
Msa0265200 AT4G24770.2 39.000 100 57 2 25 123 137 233 3.61e-12 62.4
Msa0265200 AT3G26420.1 44.944 89 48 1 39 126 8 96 1.38e-18 79.0
Msa0265200 AT2G37510.1 48.052 77 39 1 35 110 31 107 8.08e-18 74.7
Msa0265200 AT5G50250.1 44.304 79 43 1 39 116 208 286 9.13e-18 77.4
Msa0265200 AT5G50250.1 43.678 87 45 2 38 123 113 196 1.13e-12 63.5
Msa0265200 AT4G13860.1 46.667 75 39 1 37 110 2 76 9.42e-17 70.5
Msa0265200 AT1G60000.1 48.193 83 42 1 36 117 175 257 1.26e-16 73.9
Msa0265200 AT3G46020.1 42.500 80 45 1 37 115 6 85 2.16e-16 70.1
Msa0265200 AT5G54580.1 42.683 82 46 1 37 117 55 136 8.18e-16 69.7
Msa0265200 AT3G20930.1 36.842 95 59 1 35 128 279 373 1.14e-15 72.4
Msa0265200 AT1G76460.1 38.462 91 53 1 38 128 24 111 1.19e-15 71.6
Msa0265200 AT1G48920.1 39.806 103 57 1 37 134 296 398 2.30e-15 72.0
Msa0265200 AT5G55550.5 43.038 79 44 1 39 117 7 84 2.71e-15 71.6
Msa0265200 AT5G55550.4 43.038 79 44 1 39 117 7 84 2.71e-15 71.6
Msa0265200 AT5G55550.9 43.038 79 44 1 39 117 7 84 2.71e-15 71.6
Msa0265200 AT5G55550.7 43.038 79 44 1 39 117 7 84 2.71e-15 71.6
Msa0265200 AT5G55550.1 43.038 79 44 1 39 117 7 84 2.71e-15 71.6
Msa0265200 AT5G55550.2 43.038 79 44 1 39 117 7 84 2.96e-15 71.6
Msa0265200 AT5G55550.3 43.038 79 44 1 39 117 7 84 2.96e-15 71.6
Msa0265200 AT5G55550.6 43.038 79 44 1 39 117 7 84 2.96e-15 71.6
Msa0265200 AT5G55550.11 43.038 79 44 1 39 117 7 84 2.96e-15 71.6
Msa0265200 AT1G60650.2 38.235 102 58 2 37 133 11 112 4.09e-15 70.5
Msa0265200 AT1G60650.1 38.235 102 58 2 37 133 11 112 4.09e-15 70.5
Msa0265200 AT3G52380.1 41.573 89 51 1 36 123 217 305 5.55e-15 70.1
Msa0265200 AT1G22330.1 37.500 96 57 1 38 133 17 109 5.61e-15 70.1
Msa0265200 AT4G39260.2 40.217 92 54 1 39 129 7 98 6.28e-15 67.0
Msa0265200 AT5G55550.10 45.714 70 37 1 39 108 7 75 8.03e-15 70.1
Msa0265200 AT5G55550.8 45.714 70 37 1 39 108 7 75 8.30e-15 70.1
Msa0265200 AT2G22100.1 37.778 90 56 0 33 122 158 247 1.43e-14 69.3
Msa0265200 AT4G39260.1 40.217 92 54 1 39 129 7 98 1.56e-14 67.0
Msa0265200 AT3G07810.1 41.026 78 45 1 39 116 7 83 1.64e-14 69.3
Msa0265200 AT3G07810.2 41.026 78 45 1 39 116 7 83 1.73e-14 69.3
Msa0265200 AT1G54080.1 43.038 79 44 1 36 113 146 224 1.92e-14 68.9
Msa0265200 AT1G22910.2 38.961 77 47 0 38 114 13 89 2.04e-14 67.8
Msa0265200 AT2G46780.1 37.234 94 54 1 38 126 22 115 2.12e-14 68.6
Msa0265200 AT2G46780.2 37.234 94 54 1 38 126 22 115 2.12e-14 68.6
Msa0265200 AT1G22910.1 38.961 77 47 0 38 114 13 89 2.17e-14 67.8
Msa0265200 AT1G33470.2 42.029 69 40 0 38 106 7 75 2.32e-14 67.8
Msa0265200 AT1G33470.3 42.029 69 40 0 38 106 7 75 2.43e-14 67.8
Msa0265200 AT1G33470.1 42.029 69 40 0 38 106 7 75 2.60e-14 67.8
Msa0265200 AT3G14100.1 44.000 75 41 1 40 113 146 220 2.65e-14 68.6
Msa0265200 AT5G04280.1 39.744 78 46 1 38 114 7 84 2.95e-14 68.2
Msa0265200 AT2G18510.1 41.573 89 50 2 38 124 112 200 3.29e-14 68.2
Msa0265200 AT4G26650.1 42.308 78 44 1 39 116 16 92 3.57e-14 68.6
Msa0265200 AT4G26650.1 31.034 87 60 0 37 123 121 207 1.99e-11 60.5
Msa0265200 AT4G26650.2 42.308 78 44 1 39 116 13 89 3.78e-14 68.2
Msa0265200 AT4G26650.2 31.034 87 60 0 37 123 118 204 2.06e-11 60.5
Msa0265200 AT1G20880.2 38.961 77 47 0 38 114 24 100 4.16e-14 67.4
Msa0265200 AT1G20880.3 38.961 77 47 0 38 114 24 100 4.16e-14 67.4
Msa0265200 AT1G20880.1 38.961 77 47 0 38 114 24 100 4.16e-14 67.4
Msa0265200 AT1G22910.3 38.961 77 47 0 38 114 13 89 5.75e-14 67.4
Msa0265200 AT4G13860.2 53.448 58 26 1 54 110 2 59 7.27e-14 62.4
Msa0265200 AT5G47620.1 39.759 83 49 1 34 116 2 83 7.47e-14 67.4
Msa0265200 AT5G47620.1 33.333 78 52 0 36 113 104 181 2.57e-11 60.1
Msa0265200 AT5G47620.2 39.759 83 49 1 34 116 2 83 7.47e-14 67.4
Msa0265200 AT5G47620.2 33.333 78 52 0 36 113 104 181 2.57e-11 60.1
Msa0265200 AT5G47620.5 39.759 83 49 1 34 116 2 83 7.47e-14 67.4
Msa0265200 AT5G47620.5 33.333 78 52 0 36 113 104 181 2.57e-11 60.1
Msa0265200 AT1G78260.1 36.364 77 49 0 38 114 17 93 1.31e-13 66.2
Msa0265200 AT5G47620.4 46.154 65 35 0 34 98 2 66 1.33e-13 66.6
Msa0265200 AT5G47620.4 33.333 78 52 0 36 113 126 203 2.56e-11 60.1
Msa0265200 AT4G39260.4 38.983 59 36 0 39 97 7 65 2.00e-13 62.4
Msa0265200 AT1G78260.2 36.364 77 49 0 38 114 17 93 2.04e-13 65.5
Msa0265200 AT3G13224.3 36.667 90 56 1 30 118 92 181 2.05e-13 65.9
Msa0265200 AT3G13224.2 36.667 90 56 1 30 118 101 190 2.27e-13 65.9
Msa0265200 AT3G54770.3 36.364 77 49 0 38 114 17 93 3.10e-13 64.7
Msa0265200 AT2G27330.1 31.579 95 64 1 37 130 20 114 3.35e-13 62.0
Msa0265200 AT3G54770.1 36.364 77 49 0 38 114 17 93 3.54e-13 64.7
Msa0265200 AT1G17640.2 34.694 98 61 2 36 131 155 251 3.78e-13 65.5
Msa0265200 AT1G17640.1 34.694 98 61 2 36 131 155 251 3.78e-13 65.5
Msa0265200 AT1G17640.3 34.694 98 61 2 36 131 155 251 3.78e-13 65.5
Msa0265200 AT3G13224.1 45.588 68 37 0 30 97 101 168 4.10e-13 64.3
Msa0265200 AT2G21690.1 39.437 71 42 1 41 110 10 80 5.99e-13 61.6
Msa0265200 AT4G14300.1 40.506 79 46 1 39 117 7 84 8.88e-13 64.3
Msa0265200 AT4G14300.1 34.146 82 54 0 37 118 109 190 3.16e-12 62.8
Msa0265200 AT4G14300.2 40.506 79 46 1 39 117 7 84 8.88e-13 64.3
Msa0265200 AT4G14300.2 34.146 82 54 0 37 118 109 190 3.16e-12 62.8
Msa0265200 AT1G17370.2 44.000 75 41 1 40 113 141 215 9.01e-13 64.3
Msa0265200 AT2G33410.1 31.579 114 72 1 11 118 77 190 9.80e-13 64.3
Msa0265200 AT2G33410.1 37.349 83 51 1 39 121 7 88 1.35e-12 63.9
Msa0265200 AT1G17370.1 44.000 75 41 1 40 113 141 215 1.02e-12 64.3
Msa0265200 AT1G49760.1 43.590 78 43 1 37 113 44 121 1.25e-12 63.9
Msa0265200 AT1G49760.2 43.590 78 43 1 37 113 44 121 1.25e-12 63.9
Msa0265200 AT1G71800.1 35.443 79 49 2 40 116 11 89 1.38e-12 63.9
Msa0265200 AT1G01080.1 37.975 79 49 0 32 110 206 284 1.78e-12 63.2
Msa0265200 AT1G01080.2 37.975 79 49 0 32 110 207 285 1.81e-12 63.2
Msa0265200 AT1G54080.2 40.964 83 44 2 36 113 146 228 2.00e-12 63.2
Msa0265200 AT4G00830.2 36.250 80 50 1 38 116 116 195 2.39e-12 63.2
Msa0265200 AT4G00830.1 36.250 80 50 1 38 116 116 195 2.39e-12 63.2
Msa0265200 AT4G00830.6 36.250 80 50 1 38 116 116 195 2.39e-12 63.2
Msa0265200 AT4G00830.3 36.250 80 50 1 38 116 116 195 2.57e-12 63.2
Msa0265200 AT4G00830.5 36.250 80 50 1 38 116 116 195 2.57e-12 63.2
Msa0265200 AT2G37510.2 36.275 102 39 2 35 110 31 132 3.11e-12 60.8
Msa0265200 AT2G19380.1 33.333 84 56 0 33 116 403 486 3.24e-12 62.8
Msa0265200 AT1G73530.1 33.333 81 53 1 37 116 76 156 4.68e-12 60.5
Msa0265200 AT5G53720.1 36.364 77 49 0 37 113 8 84 4.73e-12 58.5
Msa0265200 AT5G11412.1 34.211 76 50 0 38 113 52 127 4.87e-12 60.5
Msa0265200 AT4G03110.1 36.471 85 53 1 38 121 349 433 5.20e-12 62.0
Msa0265200 AT4G20030.1 34.286 105 60 4 26 125 31 131 5.56e-12 59.7
Msa0265200 AT3G18610.1 39.535 86 49 2 37 122 383 465 7.27e-12 62.0
Msa0265200 AT3G18610.3 39.535 86 49 2 37 122 353 435 1.08e-11 61.2
Msa0265200 AT5G53680.1 34.615 78 49 1 38 113 13 90 1.27e-11 59.3
Msa0265200 AT3G18610.2 39.535 86 49 2 37 122 323 405 1.54e-11 60.8
Msa0265200 AT5G47620.3 33.766 77 51 0 37 113 45 121 1.75e-11 60.5
Msa0265200 AT1G47490.2 37.234 94 57 2 40 131 199 292 1.82e-11 60.5
Msa0265200 AT5G51300.1 36.585 82 51 1 33 113 475 556 1.93e-11 60.5
Msa0265200 AT5G51300.2 36.585 82 51 1 33 113 475 556 1.93e-11 60.5
Msa0265200 AT5G51300.3 36.585 82 51 1 33 113 475 556 1.93e-11 60.5
Msa0265200 AT1G47490.1 37.234 94 57 2 40 131 199 292 2.60e-11 60.1
Msa0265200 AT3G15010.1 40.278 72 43 0 35 106 72 143 3.41e-11 59.7
Msa0265200 AT3G15010.2 40.278 72 43 0 35 106 72 143 3.41e-11 59.7
Msa0265200 AT5G54900.1 39.506 81 47 2 40 118 156 236 3.52e-11 59.7
Msa0265200 AT5G40490.2 32.927 82 54 1 37 117 112 193 3.80e-11 59.7
Msa0265200 AT1G13690.1 37.333 75 46 1 40 113 15 89 4.65e-11 57.8
Msa0265200 AT3G10400.1 37.975 79 48 1 38 115 57 135 4.88e-11 58.9
Msa0265200 AT1G47500.2 35.789 95 59 2 40 132 201 295 9.07e-11 58.5
Msa0265200 AT1G47500.1 35.789 95 59 2 40 132 201 295 9.33e-11 58.5

Find 48 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGATGGACAGGATTTAAA+TGG 0.218034 2_2:-65432387 Msa0265200:CDS
ACTGGAAGATCAAGGGGATT+TGG 0.334462 2_2:-65432456 Msa0265200:CDS
GGTGGCTACGGCGGTGGTGG+CGG 0.347636 2_2:-65432306 Msa0265200:CDS
GGCTACGGCGGTGGTGGCGG+CGG 0.365763 2_2:-65432303 Msa0265200:CDS
ATGGGTTTCTGTAACAAGAT+TGG 0.366720 2_2:-65432678 Msa0265200:CDS
TGCAACTTCAGCACTCTCTA+TGG 0.384662 2_2:-65432406 Msa0265200:CDS
GAGGAGGAGCTTGAGTGCTC+TGG 0.408995 2_2:+65432631 None:intergenic
TACGGCGGTGGTGGCGGCGG+TGG 0.430700 2_2:-65432300 Msa0265200:CDS
CCCGGAGGTGGCTACGGCGG+TGG 0.433180 2_2:-65432312 Msa0265200:CDS
GCGGTGGTGGCGGCGGTGGT+TGG 0.434864 2_2:-65432296 Msa0265200:CDS
TGTTGATGATCAGTCTCTTA+AGG 0.437494 2_2:-65432535 Msa0265200:CDS
ACCACCGCCGTAGCCACCTC+CGG 0.444246 2_2:+65432311 None:intergenic
GGAGGTGGCTACGGCGGTGG+TGG 0.453741 2_2:-65432309 Msa0265200:CDS
TAGTTAAGCATGGATGAAAC+AGG 0.461015 2_2:+65432609 None:intergenic
AGCACTCTCTATGGATGGAC+AGG 0.475981 2_2:-65432397 Msa0265200:CDS
GGTGGACCTCGTCCCGGAGG+TGG 0.479282 2_2:-65432324 Msa0265200:CDS
CCTCGTCCCGGAGGTGGCTA+CGG 0.490162 2_2:-65432318 Msa0265200:CDS
GATCATCAACATGGTAAGAA+AGG 0.505312 2_2:+65432547 None:intergenic
ATAGAGACACTGGAAGATCA+AGG 0.517492 2_2:-65432464 Msa0265200:CDS
CCGTAGCCACCTCCGGGACG+AGG 0.524085 2_2:+65432318 None:intergenic
AGGAGGAGCTTGAGTGCTCT+GGG 0.530346 2_2:+65432632 None:intergenic
CAGGCTGGTGGACCTCGTCC+CGG 0.532193 2_2:-65432330 Msa0265200:CDS
TGGATGGACAGGATTTAAAT+GGG 0.532473 2_2:-65432386 Msa0265200:CDS
AAGGATGCATTTACAACCTA+CGG 0.532917 2_2:-65432516 Msa0265200:CDS
GGGTACCACCTTGCCTCAAG+AGG 0.543148 2_2:+65432652 None:intergenic
GTCCTATGCAAATGATAGAC+AGG 0.554294 2_2:-65432349 Msa0265200:CDS
CCACCGCCGTAGCCACCTCC+GGG 0.558233 2_2:+65432312 None:intergenic
GTTATCACTGATAGAGACAC+TGG 0.581409 2_2:-65432474 Msa0265200:CDS
ACTTCAGCACTCTCTATGGA+TGG 0.581897 2_2:-65432402 Msa0265200:CDS
TATGCAAATGATAGACAGGC+TGG 0.581925 2_2:-65432345 Msa0265200:CDS
TGCCTCAACCACTTCTCCGT+AGG 0.589946 2_2:+65432500 None:intergenic
TAGAGACACTGGAAGATCAA+GGG 0.594765 2_2:-65432463 Msa0265200:CDS
AGAGACACTGGAAGATCAAG+GGG 0.604078 2_2:-65432462 Msa0265200:CDS
ATTTACAACCTACGGAGAAG+TGG 0.607456 2_2:-65432508 Msa0265200:CDS
AGCATGGATGAAACAGGAGG+AGG 0.619418 2_2:+65432615 None:intergenic
AACCTACGGAGAAGTGGTTG+AGG 0.621999 2_2:-65432502 Msa0265200:CDS
GTGGCGCAAGTAGTTAAGCA+TGG 0.622132 2_2:+65432599 None:intergenic
ATTGGAAACCTCTTGAGGCA+AGG 0.631105 2_2:-65432660 Msa0265200:CDS
CGTCCCGGAGGTGGCTACGG+CGG 0.653420 2_2:-65432315 Msa0265200:CDS
TGAAGTTGCAGACTCTTGAG+TGG 0.654691 2_2:+65432419 None:intergenic
AGCCTGTCTATCATTTGCAT+AGG 0.655393 2_2:+65432347 None:intergenic
ACAAGATTGGAAACCTCTTG+AGG 0.658250 2_2:-65432665 Msa0265200:CDS
GCAAATGATAGACAGGCTGG+TGG 0.673979 2_2:-65432342 Msa0265200:CDS
GCTGGTGGACCTCGTCCCGG+AGG 0.683775 2_2:-65432327 Msa0265200:CDS
GGAAACCTCTTGAGGCAAGG+TGG 0.688707 2_2:-65432657 Msa0265200:CDS
TAAGAGACTGATCATCAACA+TGG 0.702631 2_2:+65432538 None:intergenic
TTAAGCATGGATGAAACAGG+AGG 0.710474 2_2:+65432612 None:intergenic
AAAGCTTGCTTGAAGACATG+TGG 0.718559 2_2:+65432580 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGGGTTTCTGTAACAAGAT+TGG - chr2_2:65432293-65432312 Msa0265200:CDS 35.0%
TAGTTAAGCATGGATGAAAC+AGG + chr2_2:65432365-65432384 None:intergenic 35.0%
! TCTTCAAGCAAGCTTTTCAT+TGG - chr2_2:65432398-65432417 Msa0265200:CDS 35.0%
GATCATCAACATGGTAAGAA+AGG + chr2_2:65432427-65432446 None:intergenic 35.0%
TAAGAGACTGATCATCAACA+TGG + chr2_2:65432436-65432455 None:intergenic 35.0%
!! TGTTGATGATCAGTCTCTTA+AGG - chr2_2:65432436-65432455 Msa0265200:CDS 35.0%
AAGGATGCATTTACAACCTA+CGG - chr2_2:65432455-65432474 Msa0265200:CDS 35.0%
ATGGATGGACAGGATTTAAA+TGG - chr2_2:65432584-65432603 Msa0265200:CDS 35.0%
TGGATGGACAGGATTTAAAT+GGG - chr2_2:65432585-65432604 Msa0265200:CDS 35.0%
ACAAGATTGGAAACCTCTTG+AGG - chr2_2:65432306-65432325 Msa0265200:CDS 40.0%
TTAAGCATGGATGAAACAGG+AGG + chr2_2:65432362-65432381 None:intergenic 40.0%
! AAAGCTTGCTTGAAGACATG+TGG + chr2_2:65432394-65432413 None:intergenic 40.0%
! TCAAGCAAGCTTTTCATTGG+AGG - chr2_2:65432401-65432420 Msa0265200:CDS 40.0%
ATTTACAACCTACGGAGAAG+TGG - chr2_2:65432463-65432482 Msa0265200:CDS 40.0%
GTTATCACTGATAGAGACAC+TGG - chr2_2:65432497-65432516 Msa0265200:CDS 40.0%
ATAGAGACACTGGAAGATCA+AGG - chr2_2:65432507-65432526 Msa0265200:CDS 40.0%
TAGAGACACTGGAAGATCAA+GGG - chr2_2:65432508-65432527 Msa0265200:CDS 40.0%
GTCCTATGCAAATGATAGAC+AGG - chr2_2:65432622-65432641 Msa0265200:CDS 40.0%
AGCCTGTCTATCATTTGCAT+AGG + chr2_2:65432627-65432646 None:intergenic 40.0%
TATGCAAATGATAGACAGGC+TGG - chr2_2:65432626-65432645 Msa0265200:CDS 40.0%
ATTGGAAACCTCTTGAGGCA+AGG - chr2_2:65432311-65432330 Msa0265200:CDS 45.0%
AGAGACACTGGAAGATCAAG+GGG - chr2_2:65432509-65432528 Msa0265200:CDS 45.0%
ACTGGAAGATCAAGGGGATT+TGG - chr2_2:65432515-65432534 Msa0265200:CDS 45.0%
TGAAGTTGCAGACTCTTGAG+TGG + chr2_2:65432555-65432574 None:intergenic 45.0%
TGCAACTTCAGCACTCTCTA+TGG - chr2_2:65432565-65432584 Msa0265200:CDS 45.0%
ACTTCAGCACTCTCTATGGA+TGG - chr2_2:65432569-65432588 Msa0265200:CDS 45.0%
AGCATGGATGAAACAGGAGG+AGG + chr2_2:65432359-65432378 None:intergenic 50.0%
GTGGCGCAAGTAGTTAAGCA+TGG + chr2_2:65432375-65432394 None:intergenic 50.0%
AACCTACGGAGAAGTGGTTG+AGG - chr2_2:65432469-65432488 Msa0265200:CDS 50.0%
AGCACTCTCTATGGATGGAC+AGG - chr2_2:65432574-65432593 Msa0265200:CDS 50.0%
GCAAATGATAGACAGGCTGG+TGG - chr2_2:65432629-65432648 Msa0265200:CDS 50.0%
GGAAACCTCTTGAGGCAAGG+TGG - chr2_2:65432314-65432333 Msa0265200:CDS 55.0%
AGGAGGAGCTTGAGTGCTCT+GGG + chr2_2:65432342-65432361 None:intergenic 55.0%
TGCCTCAACCACTTCTCCGT+AGG + chr2_2:65432474-65432493 None:intergenic 55.0%
GGGTACCACCTTGCCTCAAG+AGG + chr2_2:65432322-65432341 None:intergenic 60.0%
GAGGAGGAGCTTGAGTGCTC+TGG + chr2_2:65432343-65432362 None:intergenic 60.0%
! CAGGCTGGTGGACCTCGTCC+CGG - chr2_2:65432641-65432660 Msa0265200:CDS 70.0%
CCTCGTCCCGGAGGTGGCTA+CGG - chr2_2:65432653-65432672 Msa0265200:CDS 70.0%
ACCACCGCCGTAGCCACCTC+CGG + chr2_2:65432663-65432682 None:intergenic 70.0%
! GCTGGTGGACCTCGTCCCGG+AGG - chr2_2:65432644-65432663 Msa0265200:CDS 75.0%
GGTGGACCTCGTCCCGGAGG+TGG - chr2_2:65432647-65432666 Msa0265200:CDS 75.0%
CCGTAGCCACCTCCGGGACG+AGG + chr2_2:65432656-65432675 None:intergenic 75.0%
CGTCCCGGAGGTGGCTACGG+CGG - chr2_2:65432656-65432675 Msa0265200:CDS 75.0%
CCACCGCCGTAGCCACCTCC+GGG + chr2_2:65432662-65432681 None:intergenic 75.0%
GGAGGTGGCTACGGCGGTGG+TGG - chr2_2:65432662-65432681 Msa0265200:CDS 75.0%
! GGTGGCTACGGCGGTGGTGG+CGG - chr2_2:65432665-65432684 Msa0265200:CDS 75.0%
! CCCGGAGGTGGCTACGGCGG+TGG - chr2_2:65432659-65432678 Msa0265200:CDS 80.0%
!!! GGCTACGGCGGTGGTGGCGG+CGG - chr2_2:65432668-65432687 Msa0265200:CDS 80.0%
!!! TACGGCGGTGGTGGCGGCGG+TGG - chr2_2:65432671-65432690 Msa0265200:CDS 80.0%
!!! GCGGTGGTGGCGGCGGTGGT+TGG - chr2_2:65432675-65432694 Msa0265200:CDS 80.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 65432293 65432700 65432293 ID=Msa0265200;Name=Msa0265200
chr2_2 mRNA 65432293 65432700 65432293 ID=Msa0265200-mRNA-1;Parent=Msa0265200;Name=Msa0265200-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|135
chr2_2 exon 65432293 65432700 65432293 ID=Msa0265200-mRNA-1:exon:20592;Parent=Msa0265200-mRNA-1
chr2_2 CDS 65432293 65432700 65432293 ID=Msa0265200-mRNA-1:cds;Parent=Msa0265200-mRNA-1
Gene Sequence

>Msa0265200

ATGGGTTTCTGTAACAAGATTGGAAACCTCTTGAGGCAAGGTGGTACCCAGAGCACTCAAGCTCCTCCTCCTGTTTCATCCATGCTTAACTACTTGCGCCACATGTCTTCAAGCAAGCTTTTCATTGGAGGCCTTTCTTACCATGTTGATGATCAGTCTCTTAAGGATGCATTTACAACCTACGGAGAAGTGGTTGAGGCAAGAGTTATCACTGATAGAGACACTGGAAGATCAAGGGGATTTGGATTTGTCAACTTCACCACTCAAGAGTCTGCAACTTCAGCACTCTCTATGGATGGACAGGATTTAAATGGGCGAAACATTCGTGTGTCCTATGCAAATGATAGACAGGCTGGTGGACCTCGTCCCGGAGGTGGCTACGGCGGTGGTGGCGGCGGTGGTTGGTGA
Protein sequence

>Msa0265200

MGFCNKIGNLLRQGGTQSTQAPPPVSSMLNYLRHMSSSKLFIGGLSYHVDDQSLKDAFTTYGEVVEARVITDRDTGRSRGFGFVNFTTQESATSALSMDGQDLNGRNIRVSYANDRQAGGPRPGGGYGGGGGGGW*