AlfalfaGEDB | Msa1219370

M. sativa cultivar zhongmu-4 / Msa1219370

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219370	XP_003609905.1	98.701	77	1	0	1	77	1	77	7.98e-49	158

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0579340	Msa1219370	0.809120	2.213580e-50	-8.615850e-47
Msa0650650	Msa1219370	0.805110	1.563947e-49	-8.615850e-47
Msa0650740	Msa1219370	0.804851	1.770982e-49	-8.615850e-47
Msa0797000	Msa1219370	0.809119	2.214543e-50	-8.615850e-47
Msa1131420	Msa1219370	0.805529	1.277784e-49	-8.615850e-47
Msa1206760	Msa1219370	0.818276	2.132820e-52	-8.615850e-47
Msa1206830	Msa1219370	0.825547	4.418009e-54	-8.615850e-47
Msa1209020	Msa1219370	0.922191	1.354845e-88	-8.615850e-47
Msa1219370	Msa1257100	0.816249	6.096431e-52	-8.615850e-47
Msa1219370	Msa1266840	0.989800	1.058474e-179	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219370	MtrunA17_Chr4g0069521	98.701	77	1	0	1	77	1	77	7.34e-53	158
Msa1219370	MtrunA17_Chr2g0302061	77.922	77	13	3	1	76	1	74	3.16e-36	116

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219370	AT1G48330.1	65.152	66	20	3	1	63	1	66	1.32e-23	85.1
Msa1219370	AT3G17580.1	60.274	73	17	4	1	63	1	71	3.42e-21	79.3

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Find 55 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTGTTTGAGACTTCTT+AGG	0.295118	8_1:+82188347	None:intergenic
AAGGTGAAGTAATGGCATGT+GGG	0.724189	8_1:-82188025	Msa1219370:CDS
ACAATCTTAACCTCATCTCA+CGG	0.636961	8_1:-82188619	Msa1219370:five_prime_UTR
ACACAGAGAGAAGGAAAGAG+AGG	0.582028	8_1:+82188588	None:intergenic
ACACTCTGTGGCTGTGGATT+TGG	0.258834	8_1:+82187962	None:intergenic
ACATTCGCTAATTTATGCAC+TGG	0.461046	8_1:+82187894	None:intergenic
ACCAAAACCAAATGGCTTCT+TGG	0.316100	8_1:-82188439	Msa1219370:exon
AGAACAAGAGAGGCGTATAA+TGG	0.401789	8_1:-82188054	Msa1219370:CDS
AGGCGTATAATGGATCTGCA+AGG	0.696517	8_1:-82188044	Msa1219370:CDS
ATATTCTTTCACCAATCACG+GGG	0.713644	8_1:+82187677	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GCAAATGAATGTATTCTCTA+GGG	+	chr8_1:82187769-82187788	None:intergenic	30.0%
TGCAAATGAATGTATTCTCT+AGG	+	chr8_1:82187770-82187789	None:intergenic	30.0%
TTAAAGAACAACCAACAACA+AGG	-	chr8_1:82187906-82187925	Msa1219370:three_prime_UTR	30.0%
TAAAGAACAACCAACAACAA+GGG	-	chr8_1:82187907-82187926	Msa1219370:three_prime_UTR	30.0%
TATTGTTTACAGAACAAGAG+AGG	-	chr8_1:82188215-82188234	Msa1219370:intron	30.0%
TTGATGAAGAAGAATTAAGG+TGG	-	chr8_1:82188526-82188545	Msa1219370:five_prime_UTR	30.0%
GTCAAAATGTTAGTACTTGT+TGG	-	chr8_1:82188575-82188594	Msa1219370:five_prime_UTR	30.0%
TGATATTCTTTCACCAATCA+CGG	+	chr8_1:82188607-82188626	None:intergenic	30.0%
TGATTGGTGAAAGAATATCA+TGG	-	chr8_1:82188607-82188626	Msa1219370:five_prime_UTR	30.0%
TGTAAAAGAACCAAACACAA+GGG	+	chr8_1:82188709-82188728	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	82187573	82188728	82187573	ID=Msa1219370;Name=Msa1219370
chr8_1	mRNA	82187573	82188728	82187573	ID=Msa1219370-mRNA-1;Parent=Msa1219370;Name=Msa1219370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=278\|1\|1\|1\|1\|1\|2\|368\|77
chr8_1	exon	82187573	82188074	82187573	ID=Msa1219370-mRNA-1:exon:29179;Parent=Msa1219370-mRNA-1
chr8_1	exon	82188351	82188728	82188351	ID=Msa1219370-mRNA-1:exon:29178;Parent=Msa1219370-mRNA-1
chr8_1	five_prime_UTR	82188451	82188728	82188451	ID=Msa1219370-mRNA-1:five_prime_utr;Parent=Msa1219370-mRNA-1
chr8_1	CDS	82188351	82188450	82188351	ID=Msa1219370-mRNA-1:cds;Parent=Msa1219370-mRNA-1
chr8_1	CDS	82187941	82188074	82187941	ID=Msa1219370-mRNA-1:cds;Parent=Msa1219370-mRNA-1
chr8_1	three_prime_UTR	82187573	82187940	82187573	ID=Msa1219370-mRNA-1:three_prime_utr;Parent=Msa1219370-mRNA-1

Gene Sequence	>Msa1219370 ATGGCTTCTTGGAAGAAAACCATCGCAACACCATTCAAAAAAGCTTGTACTTTCTTTAAAGAACAACCAACAACAAGGGATCCTAAGAAGTCTCAAACAGAACAAGAGAGGCGTATAATGGATCTGCAAGGTGAAGTAATGGCATGTGGGTATGAAGATGTTCAAGTTATGTGGTCAATGCTGGATAGGTCCAAATCCACAGCCACAGAGTGTAACATAACTTCATCAAGTTGA
Protein sequence	>Msa1219370 MASWKKTIATPFKKACTFFKEQPTTRDPKKSQTEQERRIMDLQGEVMACGYEDVQVMWSMLDRSKSTATECNITSSS*