AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1450980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450980 MtrunA17_Chr4g0073811 100.000 76 0 0 1 76 1 76 2.04e-50 152
Msa1450980 MtrunA17_Chr4g0073821 81.333 75 14 0 1 75 1 75 1.20e-40 127
Msa1450980 MtrunA17_Chr6g0462381 57.692 52 21 1 5 56 3 53 4.54e-14 60.1
Msa1450980 MtrunA17_Chr7g0271271 65.217 46 15 1 10 55 8 52 1.78e-13 58.5
Msa1450980 MtrunA17_Chr7g0271261 57.447 47 19 1 9 55 7 52 6.18e-12 54.7
Msa1450980 MtrunA17_Chr7g0271281 56.522 46 19 1 10 55 8 52 8.21e-12 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1450980 AT4G30660.1 76.712 73 17 0 1 73 1 73 2.09e-37 120
Msa1450980 AT4G30660.2 76.712 73 17 0 1 73 1 73 2.09e-37 120
Msa1450980 AT4G28088.1 70.833 72 21 0 1 72 1 72 5.93e-36 116
Msa1450980 AT2G24040.1 70.833 72 21 0 1 72 1 72 8.09e-34 110
Msa1450980 AT4G30650.1 75.410 61 15 0 1 61 1 61 1.03e-27 95.5
Msa1450980 AT2G38905.1 68.627 51 15 1 6 56 4 53 3.64e-16 65.9
Msa1450980 AT3G05880.1 71.739 46 12 1 10 55 8 52 6.63e-15 62.8
Msa1450980 AT3G05890.1 58.696 46 18 1 10 55 8 52 6.31e-13 57.8
Msa1450980 AT2G24040.2 76.667 30 7 0 1 30 1 30 4.98e-12 55.5

Find 28 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGACGTTGGAGTCAATT+TGG 0.187395 tig0033155:+13045 Msa1450980:three_prime_UTR
CTATCTGATTGTTGATTGTA+TGG 0.350406 tig0033155:+13163 Msa1450980:three_prime_UTR
GTATGATATGAATATTTGAT+TGG 0.389091 tig0033155:+13253 Msa1450980:three_prime_UTR
TTCAACTCTTTATTATGTAA+TGG 0.390988 tig0033155:+12976 Msa1450980:three_prime_UTR
CGCTGTATTACTCCCTCCAT+TGG 0.403030 tig0033155:+12473 Msa1450980:CDS
GTGGCTTATTAATAGACATT+TGG 0.459610 tig0033155:+12345 Msa1450980:five_prime_UTR
TAAAGTGGTTAGTTATATTG+TGG 0.465212 tig0033155:+13224 Msa1450980:three_prime_UTR
ACAATACTAGGATATATTCC+TGG 0.469022 tig0033155:+12831 Msa1450980:CDS
ATTGGTTTATGATAGAGATA+AGG 0.489530 tig0033155:+13271 Msa1450980:three_prime_UTR
TGCTTGCTGCTTACAATACT+AGG 0.494816 tig0033155:+12819 Msa1450980:CDS
ATTGTAATTCTCATTGACGT+TGG 0.499163 tig0033155:+13034 Msa1450980:three_prime_UTR
ATCAAATTATGAAAATAAAG+TGG 0.499775 tig0033155:+13209 Msa1450980:three_prime_UTR
CAGATAATTTCACAAGTTGA+AGG 0.500488 tig0033155:-12438 Msa1450980:intergenic
GACATTTGGTGAAGATCAGT+TGG 0.514122 tig0033155:+12359 Msa1450980:five_prime_UTR
GCTGTATTACTCCCTCCATT+GGG 0.519016 tig0033155:+12474 Msa1450980:CDS
AGCCATCAACATCACTTCAT+AGG 0.526050 tig0033155:-13308 Msa1450980:intergenic
GTCCTATGAAGTGATGTTGA+TGG 0.544298 tig0033155:+13306 Msa1450980:three_prime_UTR
TTGGGTGTTTGTCTTCGCCA+TGG 0.568247 tig0033155:+12492 Msa1450980:CDS
TTGTAAGCAGCAAGCAGATG+AGG 0.568939 tig0033155:-12812 Msa1450980:intergenic
TAGTATTTGTTTGCATACAA+AGG 0.574050 tig0033155:-12921 Msa1450980:intergenic
CATCAACAAGAATAAGCAGA+AGG 0.602168 tig0033155:+12388 Msa1450980:five_prime_UTR
AGAGATAAGGTGCTATGAAG+TGG 0.607967 tig0033155:+13284 Msa1450980:three_prime_UTR
TGGCGAAGACAAACACCCAA+TGG 0.619712 tig0033155:-12489 Msa1450980:intergenic
TACATACAGTGCAGCAACCA+TGG 0.624485 tig0033155:-12509 Msa1450980:intergenic
CGAAGACAAACACCCAATGG+AGG 0.631567 tig0033155:-12486 Msa1450980:intergenic
AGCAGTACTTTGATGAAGCT+AGG 0.636728 tig0033155:+12895 Msa1450980:CDS
GAAGACAAACACCCAATGGA+GGG 0.640185 tig0033155:-12485 Msa1450970:intergenic
TAGATAGCATAAATAATACC+AGG 0.641061 tig0033155:-12849 Msa1450970:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAAAAAATCATGAAAAAA+TGG - tig0033155:12635-12654 Msa1450980:intergenic 15.0%
!!! TTTTCATGATTTTTTAGTTA+TGG + tig0033155:12637-12656 Msa1450980:intron 15.0%
!!! TTTCATGATTTTTTAGTTAT+GGG + tig0033155:12638-12657 Msa1450980:intron 15.0%
!! AGAAAAAAAAAAAATTCAAG+TGG - tig0033155:13009-13028 Msa1450980:intergenic 15.0%
!! ATCAAATTATGAAAATAAAG+TGG + tig0033155:13209-13228 Msa1450980:three_prime_UTR 15.0%
!! ACTAAAAAATCATGAAAAAA+TGG - tig0033155:12635-12654 Msa1450980:intergenic 15.0%
!!! TTTTCATGATTTTTTAGTTA+TGG + tig0033155:12637-12656 Msa1450980:intron 15.0%
!!! TTTCATGATTTTTTAGTTAT+GGG + tig0033155:12638-12657 Msa1450980:intron 15.0%
!! AGAAAAAAAAAAAATTCAAG+TGG - tig0033155:13009-13028 Msa1450980:intergenic 15.0%
!! ATCAAATTATGAAAATAAAG+TGG + tig0033155:13209-13228 Msa1450980:three_prime_UTR 15.0%
!!! AATCATGAAAAAATGGATTT+TGG - tig0033155:12628-12647 Msa1450980:intergenic 20.0%
!!! ATTGATAATTGTATTTATCC+CGG - tig0033155:12669-12688 Msa1450980:intergenic 20.0%
!! TTCAACTCTTTATTATGTAA+TGG + tig0033155:12976-12995 Msa1450980:three_prime_UTR 20.0%
!! GTATGATATGAATATTTGAT+TGG + tig0033155:13253-13272 Msa1450980:three_prime_UTR 20.0%
!!! AATCATGAAAAAATGGATTT+TGG - tig0033155:12628-12647 Msa1450980:intergenic 20.0%
!!! ATTGATAATTGTATTTATCC+CGG - tig0033155:12669-12688 Msa1450980:intergenic 20.0%
!! TTCAACTCTTTATTATGTAA+TGG + tig0033155:12976-12995 Msa1450980:three_prime_UTR 20.0%
!! GTATGATATGAATATTTGAT+TGG + tig0033155:13253-13272 Msa1450980:three_prime_UTR 20.0%
! GAAAGAAAGTGAGATTAAAA+AGG - tig0033155:12569-12588 Msa1450980:intergenic 25.0%
! AAAGGGAAAAAGAAGTAAAA+GGG - tig0033155:12598-12617 Msa1450980:intergenic 25.0%
! AAAAGGGAAAAAGAAGTAAA+AGG - tig0033155:12599-12618 Msa1450980:intergenic 25.0%
! TCAATCTCAAATTTATGATG+AGG + tig0033155:12684-12703 Msa1450980:intron 25.0%
! ATTGAAATCAAAGTCATTCA+TGG - tig0033155:12771-12790 Msa1450980:intergenic 25.0%
! TAGATAGCATAAATAATACC+AGG - tig0033155:12852-12871 Msa1450980:intergenic 25.0%
! TAGTATTTGTTTGCATACAA+AGG - tig0033155:12924-12943 Msa1450980:intergenic 25.0%
!! TAAAGTGGTTAGTTATATTG+TGG + tig0033155:13224-13243 Msa1450980:three_prime_UTR 25.0%
!! ATTGGTTTATGATAGAGATA+AGG + tig0033155:13271-13290 Msa1450980:three_prime_UTR 25.0%
! GAAAGAAAGTGAGATTAAAA+AGG - tig0033155:12569-12588 Msa1450980:intergenic 25.0%
! AAAGGGAAAAAGAAGTAAAA+GGG - tig0033155:12598-12617 Msa1450980:intergenic 25.0%
! AAAAGGGAAAAAGAAGTAAA+AGG - tig0033155:12599-12618 Msa1450980:intergenic 25.0%
! TCAATCTCAAATTTATGATG+AGG + tig0033155:12684-12703 Msa1450980:intron 25.0%
! ATTGAAATCAAAGTCATTCA+TGG - tig0033155:12771-12790 Msa1450980:intergenic 25.0%
! TAGATAGCATAAATAATACC+AGG - tig0033155:12852-12871 Msa1450980:intergenic 25.0%
! TAGTATTTGTTTGCATACAA+AGG - tig0033155:12924-12943 Msa1450980:intergenic 25.0%
!! TAAAGTGGTTAGTTATATTG+TGG + tig0033155:13224-13243 Msa1450980:three_prime_UTR 25.0%
!! ATTGGTTTATGATAGAGATA+AGG + tig0033155:13271-13290 Msa1450980:three_prime_UTR 25.0%
GTGGCTTATTAATAGACATT+TGG + tig0033155:12345-12364 Msa1450980:five_prime_UTR 30.0%
! CAGATAATTTCACAAGTTGA+AGG - tig0033155:12441-12460 Msa1450980:intergenic 30.0%
AAGGGAAAAAGAAGTAAAAG+GGG - tig0033155:12597-12616 Msa1450980:intergenic 30.0%
! TGGATTTTGGATGTGAAAAA+GGG - tig0033155:12615-12634 Msa1450980:intergenic 30.0%
! ATGGATTTTGGATGTGAAAA+AGG - tig0033155:12616-12635 Msa1450980:intergenic 30.0%
!!! TTTTTAGTTATGGGTCAAAC+CGG + tig0033155:12647-12666 Msa1450980:intron 30.0%
ACAATACTAGGATATATTCC+TGG + tig0033155:12831-12850 Msa1450980:CDS 30.0%
ATTGTAATTCTCATTGACGT+TGG + tig0033155:13034-13053 Msa1450980:three_prime_UTR 30.0%
!! TTAGACTTTTAGTTGAGAAC+AGG + tig0033155:13095-13114 Msa1450980:three_prime_UTR 30.0%
! CTATCTGATTGTTGATTGTA+TGG + tig0033155:13163-13182 Msa1450980:three_prime_UTR 30.0%
GTGGCTTATTAATAGACATT+TGG + tig0033155:12345-12364 Msa1450980:five_prime_UTR 30.0%
! CAGATAATTTCACAAGTTGA+AGG - tig0033155:12441-12460 Msa1450980:intergenic 30.0%
AAGGGAAAAAGAAGTAAAAG+GGG - tig0033155:12597-12616 Msa1450980:intergenic 30.0%
! TGGATTTTGGATGTGAAAAA+GGG - tig0033155:12615-12634 Msa1450980:intergenic 30.0%
! ATGGATTTTGGATGTGAAAA+AGG - tig0033155:12616-12635 Msa1450980:intergenic 30.0%
!!! TTTTTAGTTATGGGTCAAAC+CGG + tig0033155:12647-12666 Msa1450980:intron 30.0%
ACAATACTAGGATATATTCC+TGG + tig0033155:12831-12850 Msa1450980:CDS 30.0%
ATTGTAATTCTCATTGACGT+TGG + tig0033155:13034-13053 Msa1450980:three_prime_UTR 30.0%
!! TTAGACTTTTAGTTGAGAAC+AGG + tig0033155:13095-13114 Msa1450980:three_prime_UTR 30.0%
! CTATCTGATTGTTGATTGTA+TGG + tig0033155:13163-13182 Msa1450980:three_prime_UTR 30.0%
CATCAACAAGAATAAGCAGA+AGG + tig0033155:12388-12407 Msa1450980:five_prime_UTR 35.0%
!! TTTTAGTTATGGGTCAAACC+GGG + tig0033155:12648-12667 Msa1450980:intron 35.0%
CATCAACAAGAATAAGCAGA+AGG + tig0033155:12388-12407 Msa1450980:five_prime_UTR 35.0%
!! TTTTAGTTATGGGTCAAACC+GGG + tig0033155:12648-12667 Msa1450980:intron 35.0%
! GACATTTGGTGAAGATCAGT+TGG + tig0033155:12359-12378 Msa1450980:five_prime_UTR 40.0%
! TGCTTGCTGCTTACAATACT+AGG + tig0033155:12819-12838 Msa1450980:CDS 40.0%
AGCAGTACTTTGATGAAGCT+AGG + tig0033155:12895-12914 Msa1450980:CDS 40.0%
CATTGACGTTGGAGTCAATT+TGG + tig0033155:13045-13064 Msa1450980:three_prime_UTR 40.0%
! AGAGATAAGGTGCTATGAAG+TGG + tig0033155:13284-13303 Msa1450980:three_prime_UTR 40.0%
GTCCTATGAAGTGATGTTGA+TGG + tig0033155:13306-13325 Msa1450980:three_prime_UTR 40.0%
! GACATTTGGTGAAGATCAGT+TGG + tig0033155:12359-12378 Msa1450980:five_prime_UTR 40.0%
! TGCTTGCTGCTTACAATACT+AGG + tig0033155:12819-12838 Msa1450980:CDS 40.0%
AGCAGTACTTTGATGAAGCT+AGG + tig0033155:12895-12914 Msa1450980:CDS 40.0%
CATTGACGTTGGAGTCAATT+TGG + tig0033155:13045-13064 Msa1450980:three_prime_UTR 40.0%
! AGAGATAAGGTGCTATGAAG+TGG + tig0033155:13284-13303 Msa1450980:three_prime_UTR 40.0%
GCTGTATTACTCCCTCCATT+GGG + tig0033155:12474-12493 Msa1450980:CDS 45.0%
GAAGACAAACACCCAATGGA+GGG - tig0033155:12488-12507 Msa1450980:intergenic 45.0%
TACATACAGTGCAGCAACCA+TGG - tig0033155:12512-12531 Msa1450980:intergenic 45.0%
TTGTAAGCAGCAAGCAGATG+AGG - tig0033155:12815-12834 Msa1450980:intergenic 45.0%
GCTGTATTACTCCCTCCATT+GGG + tig0033155:12474-12493 Msa1450980:CDS 45.0%
GAAGACAAACACCCAATGGA+GGG - tig0033155:12488-12507 Msa1450980:intergenic 45.0%
TACATACAGTGCAGCAACCA+TGG - tig0033155:12512-12531 Msa1450980:intergenic 45.0%
TTGTAAGCAGCAAGCAGATG+AGG - tig0033155:12815-12834 Msa1450980:intergenic 45.0%
CGCTGTATTACTCCCTCCAT+TGG + tig0033155:12473-12492 Msa1450980:CDS 50.0%
CGAAGACAAACACCCAATGG+AGG - tig0033155:12489-12508 Msa1450980:intergenic 50.0%
TGGCGAAGACAAACACCCAA+TGG - tig0033155:12492-12511 Msa1450980:intergenic 50.0%
TTGGGTGTTTGTCTTCGCCA+TGG + tig0033155:12492-12511 Msa1450980:CDS 50.0%
CGCTGTATTACTCCCTCCAT+TGG + tig0033155:12473-12492 Msa1450980:CDS 50.0%
CGAAGACAAACACCCAATGG+AGG - tig0033155:12489-12508 Msa1450980:intergenic 50.0%
TGGCGAAGACAAACACCCAA+TGG - tig0033155:12492-12511 Msa1450980:intergenic 50.0%
TTGGGTGTTTGTCTTCGCCA+TGG + tig0033155:12492-12511 Msa1450980:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0033155 gene 12345 13327 12345 ID=Msa1450980;Name=Msa1450980
tig0033155 mRNA 12345 13327 12345 ID=Msa1450980-mRNA-1;Parent=Msa1450980;Name=Msa1450980-mRNA-1;_AED=0.27;_eAED=0.27;_QI=90|1|1|1|1|1|2|383|76
tig0033155 exon 12345 12524 12345 ID=Msa1450980-mRNA-1:exon:17762;Parent=Msa1450980-mRNA-1
tig0033155 exon 12804 13327 12804 ID=Msa1450980-mRNA-1:exon:17763;Parent=Msa1450980-mRNA-1
tig0033155 five_prime_UTR 12345 12434 12345 ID=Msa1450980-mRNA-1:five_prime_utr;Parent=Msa1450980-mRNA-1
tig0033155 CDS 12435 12524 12435 ID=Msa1450980-mRNA-1:cds;Parent=Msa1450980-mRNA-1
tig0033155 CDS 12804 12944 12804 ID=Msa1450980-mRNA-1:cds;Parent=Msa1450980-mRNA-1
tig0033155 three_prime_UTR 12945 13327 12945 ID=Msa1450980-mRNA-1:three_prime_utr;Parent=Msa1450980-mRNA-1
Gene Sequence

>Msa1450980

ATGCCTTCAACTTGTGAAATTATCTGTGAGATTTTGATCGCTGTATTACTCCCTCCATTGGGTGTTTGTCTTCGCCATGGTTGCTGCACTAATCAATTCCTCATCTGCTTGCTGCTTACAATACTAGGATATATTCCTGGTATTATTTATGCTATCTATGTCATTATCTTTGTTGATCGTGAGCAGTACTTTGATGAAGCTAGGCGTCCTTTGTATGCAAACAAATACTAG

Protein sequence

>Msa1450980

MPSTCEIICEILIAVLLPPLGVCLRHGCCTNQFLICLLLTILGYIPGIIYAIYVIIFVDREQYFDEARRPLYANKY*