AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1209020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1209020 MtrunA17_Chr4g0069521 98.701 77 1 0 1 77 1 77 7.34e-53 158
Msa1209020 MtrunA17_Chr2g0302061 77.922 77 13 3 1 76 1 74 3.16e-36 116
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1209020 AT1G48330.1 65.152 66 20 3 1 63 1 66 1.32e-23 85.1
Msa1209020 AT3G17580.1 60.274 73 17 4 1 63 1 71 3.42e-21 79.3

Find 55 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAAGAAGCCATTTGGTTT+TGG 0.252906 8_1:+67406532 None:intergenic
GTGCTTATGTCCAGTCCTTT+AGG 0.254502 8_1:-67406792 Msa1209020:five_prime_UTR
GATAAGAGAAGTCAATAAAA+AGG 0.255616 8_1:-67406757 Msa1209020:five_prime_UTR
ACACTCTGTGGCTGTGGATT+TGG 0.258834 8_1:+67406056 None:intergenic
TGCATCAAATATCTTGAATA+TGG 0.276681 8_1:-67405928 Msa1209020:three_prime_UTR
AAACCTGTTTGAGACTTCTT+AGG 0.295118 8_1:+67406441 None:intergenic
ACCAAAACCAAATGGCTTCT+TGG 0.316100 8_1:-67406533 Msa1209020:exon
GTCAAAATGTTAGTACTTGT+TGG 0.336762 8_1:-67405800 Msa1209020:three_prime_UTR
TTCTTAGGATCCCTTGTTGT+TGG 0.351825 8_1:+67406456 None:intergenic
TTATATAATATATATTATAT+AGG 0.357294 8_1:+67406633 None:intergenic
TTATATGCTAATTAATGTTA+AGG 0.373939 8_1:-67405961 Msa1209020:three_prime_UTR
AGAACAAGAGAGGCGTATAA+TGG 0.401789 8_1:-67406148 Msa1209020:CDS
TCAAAATGTTAGTACTTGTT+GGG 0.424308 8_1:-67405799 Msa1209020:three_prime_UTR
TACATTCATTTGCAAAGATA+AGG 0.427064 8_1:-67406597 Msa1209020:five_prime_UTR
ACATTCGCTAATTTATGCAC+TGG 0.461046 8_1:+67405988 None:intergenic
TGCAAATGAATGTATTCTCT+AGG 0.462994 8_1:+67406606 None:intergenic
TTATGCACTGGGACTTCAAA+GGG 0.464235 8_1:+67406000 None:intergenic
CATTCGCTAATTTATGCACT+GGG 0.478527 8_1:+67405989 None:intergenic
GCAAATGAATGTATTCTCTA+GGG 0.489660 8_1:+67406607 None:intergenic
TATGTTACACTCTGTGGCTG+TGG 0.492905 8_1:+67406050 None:intergenic
GATCCTAAGAAGTCTCAAAC+AGG 0.499805 8_1:-67406444 Msa1209020:intron
ATGGTATGAATGTAATGTAA+TGG 0.502131 8_1:-67405909 Msa1209020:three_prime_UTR
TCAAGTTATGTGGTCAATGC+TGG 0.502431 8_1:-67406085 Msa1209020:CDS
ATCTCTCTCATCAAACCTAA+AGG 0.510453 8_1:+67406777 None:intergenic
GTCAATCAACCAAAACCAAA+TGG 0.522048 8_1:-67406541 Msa1209020:exon
TGATATTCTTTCACCAATCA+CGG 0.527224 8_1:+67405771 None:intergenic
TTGTTGGGAATCCCCGTGAT+TGG 0.536042 8_1:-67405784 Msa1209020:three_prime_UTR
GATATTCTTTCACCAATCAC+GGG 0.538061 8_1:+67405772 None:intergenic
ATGAAGATGTTCAAGTTATG+TGG 0.553111 8_1:-67406095 Msa1209020:CDS
TCTCATCAAACCTAAAGGAC+TGG 0.554967 8_1:+67406782 None:intergenic
CAAACACAAACACAGAGAGA+AGG 0.559898 8_1:+67406679 None:intergenic
GGATCTGCAAGGTGAAGTAA+TGG 0.564236 8_1:-67406127 Msa1209020:CDS
TTTATGCACTGGGACTTCAA+AGG 0.569375 8_1:+67405999 None:intergenic
TTATGTGGTCAATGCTGGAT+AGG 0.570689 8_1:-67406080 Msa1209020:CDS
GAGGAGGTTAAGATTGTGGA+AGG 0.573477 8_1:+67406725 None:intergenic
GTGAGAGGAGGTTAAGATTG+TGG 0.574082 8_1:+67406721 None:intergenic
ACACAGAGAGAAGGAAAGAG+AGG 0.582028 8_1:+67406688 None:intergenic
TACTTATTGAACCGTCAAAG+AGG 0.594531 8_1:+67405704 None:intergenic
TATTGTTTACAGAACAAGAG+AGG 0.616356 8_1:-67406158 Msa1209020:intron
GGAGCAAGATCCGTGAGAGG+AGG 0.622856 8_1:+67406709 None:intergenic
GTATAGAGAAGCATATTCAA+AGG 0.628569 8_1:-67406575 Msa1209020:five_prime_UTR
ACAATCTTAACCTCCTCTCA+CGG 0.630484 8_1:-67406719 Msa1209020:five_prime_UTR
TTGATGAAGAATTAAGGTGG+CGG 0.631704 8_1:-67405846 Msa1209020:three_prime_UTR
TTAAAGAACAACCAACAACA+AGG 0.633465 8_1:-67406467 Msa1209020:CDS
TGAAGTTATGTTACACTCTG+TGG 0.635521 8_1:+67406044 None:intergenic
AGAGGAGCAAGATCCGTGAG+AGG 0.636674 8_1:+67406706 None:intergenic
CAAGGTGAAGTAATGGCATG+TGG 0.637142 8_1:-67406120 Msa1209020:CDS
TCTTTGATGAAGAATTAAGG+TGG 0.646435 8_1:-67405849 Msa1209020:three_prime_UTR
ATGTAAAAGAACCAAACACA+AGG 0.662731 8_1:+67405667 None:intergenic
TAAAGAACAACCAACAACAA+GGG 0.675428 8_1:-67406466 Msa1209020:CDS
AGGCGTATAATGGATCTGCA+AGG 0.696517 8_1:-67406138 Msa1209020:CDS
ATATTCTTTCACCAATCACG+GGG 0.713644 8_1:+67405773 None:intergenic
TGATTGGTGAAAGAATATCA+TGG 0.714774 8_1:-67405768 Msa1209020:three_prime_UTR
TGTAAAAGAACCAAACACAA+GGG 0.715508 8_1:+67405668 None:intergenic
AAGGTGAAGTAATGGCATGT+GGG 0.724189 8_1:-67406119 Msa1209020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATAATATATATTATAT+AGG + chr8_1:67405844-67405863 None:intergenic 0.0%
!! TTATATAATATATATTATAT+AGG + chr8_1:67405844-67405863 None:intergenic 0.0%
!!! AAGTACAATTTTAAATTATA+AGG + chr8_1:67406211-67406230 None:intergenic 10.0%
!!! GTACTTTTATTTATTTTTTA+CGG - chr8_1:67406225-67406244 Msa1209020:intron 10.0%
!!! AAGTACAATTTTAAATTATA+AGG + chr8_1:67406211-67406230 None:intergenic 10.0%
!!! GTACTTTTATTTATTTTTTA+CGG - chr8_1:67406225-67406244 Msa1209020:intron 10.0%
!!! TTATTCAAATAGTATTTTGT+CGG - chr8_1:67406165-67406184 Msa1209020:intron 15.0%
!! AAAAAATGAGTTAAATAATG+TGG + chr8_1:67406296-67406315 None:intergenic 15.0%
!! TTATATGCTAATTAATGTTA+AGG - chr8_1:67406513-67406532 Msa1209020:CDS 15.0%
!!! TTATTCAAATAGTATTTTGT+CGG - chr8_1:67406165-67406184 Msa1209020:intron 15.0%
!! AAAAAATGAGTTAAATAATG+TGG + chr8_1:67406296-67406315 None:intergenic 15.0%
!! TTATATGCTAATTAATGTTA+AGG - chr8_1:67406513-67406532 Msa1209020:CDS 15.0%
!!! AAATGAAGATTTAAGATTGT+GGG + chr8_1:67406070-67406089 None:intergenic 20.0%
!!! ATTGAAAGTACAATTTCTTT+TGG + chr8_1:67406272-67406291 None:intergenic 20.0%
!! TTCATCAAAGAAAAAAAAAG+GGG + chr8_1:67406616-67406635 None:intergenic 20.0%
!! CTTCATCAAAGAAAAAAAAA+GGG + chr8_1:67406617-67406636 None:intergenic 20.0%
!! TCTTCATCAAAGAAAAAAAA+AGG + chr8_1:67406618-67406637 None:intergenic 20.0%
!!! TTTTCTTTGATGAAGAATTA+AGG - chr8_1:67406622-67406641 Msa1209020:five_prime_UTR 20.0%
!!! GTGAGATTTTTTTTAATGAA+AGG - chr8_1:67406728-67406747 Msa1209020:five_prime_UTR 20.0%
!!! AAATGAAGATTTAAGATTGT+GGG + chr8_1:67406070-67406089 None:intergenic 20.0%
!!! ATTGAAAGTACAATTTCTTT+TGG + chr8_1:67406272-67406291 None:intergenic 20.0%
!! TTCATCAAAGAAAAAAAAAG+GGG + chr8_1:67406616-67406635 None:intergenic 20.0%
!! CTTCATCAAAGAAAAAAAAA+GGG + chr8_1:67406617-67406636 None:intergenic 20.0%
!! TCTTCATCAAAGAAAAAAAA+AGG + chr8_1:67406618-67406637 None:intergenic 20.0%
!!! TTTTCTTTGATGAAGAATTA+AGG - chr8_1:67406622-67406641 Msa1209020:five_prime_UTR 20.0%
!!! GTGAGATTTTTTTTAATGAA+AGG - chr8_1:67406728-67406747 Msa1209020:five_prime_UTR 20.0%
! GATAAGAGAAGTCAATAAAA+AGG - chr8_1:67405717-67405736 Msa1209020:three_prime_UTR 25.0%
! TACATTCATTTGCAAAGATA+AGG - chr8_1:67405877-67405896 Msa1209020:three_prime_UTR 25.0%
!!! AAAGTACAAGCTTTTTTGAA+TGG + chr8_1:67405985-67406004 None:intergenic 25.0%
!! GAAATGAAGATTTAAGATTG+TGG + chr8_1:67406071-67406090 None:intergenic 25.0%
! TGCATCAAATATCTTGAATA+TGG - chr8_1:67406546-67406565 Msa1209020:five_prime_UTR 25.0%
! ATGGTATGAATGTAATGTAA+TGG - chr8_1:67406565-67406584 Msa1209020:five_prime_UTR 25.0%
! TCAAAATGTTAGTACTTGTT+GGG - chr8_1:67406675-67406694 Msa1209020:five_prime_UTR 25.0%
! GATAAGAGAAGTCAATAAAA+AGG - chr8_1:67405717-67405736 Msa1209020:three_prime_UTR 25.0%
! TACATTCATTTGCAAAGATA+AGG - chr8_1:67405877-67405896 Msa1209020:three_prime_UTR 25.0%
!!! AAAGTACAAGCTTTTTTGAA+TGG + chr8_1:67405985-67406004 None:intergenic 25.0%
!! GAAATGAAGATTTAAGATTG+TGG + chr8_1:67406071-67406090 None:intergenic 25.0%
! TGCATCAAATATCTTGAATA+TGG - chr8_1:67406546-67406565 Msa1209020:five_prime_UTR 25.0%
! ATGGTATGAATGTAATGTAA+TGG - chr8_1:67406565-67406584 Msa1209020:five_prime_UTR 25.0%
! TCAAAATGTTAGTACTTGTT+GGG - chr8_1:67406675-67406694 Msa1209020:five_prime_UTR 25.0%
GCAAATGAATGTATTCTCTA+GGG + chr8_1:67405870-67405889 None:intergenic 30.0%
TGCAAATGAATGTATTCTCT+AGG + chr8_1:67405871-67405890 None:intergenic 30.0%
! GTATAGAGAAGCATATTCAA+AGG - chr8_1:67405899-67405918 Msa1209020:three_prime_UTR 30.0%
TTAAAGAACAACCAACAACA+AGG - chr8_1:67406007-67406026 Msa1209020:three_prime_UTR 30.0%
TAAAGAACAACCAACAACAA+GGG - chr8_1:67406008-67406027 Msa1209020:three_prime_UTR 30.0%
TATTGTTTACAGAACAAGAG+AGG - chr8_1:67406316-67406335 Msa1209020:intron 30.0%
! ATGAAGATGTTCAAGTTATG+TGG - chr8_1:67406379-67406398 Msa1209020:intron 30.0%
TCTTTGATGAAGAATTAAGG+TGG - chr8_1:67406625-67406644 Msa1209020:five_prime_UTR 30.0%
GTCAAAATGTTAGTACTTGT+TGG - chr8_1:67406674-67406693 Msa1209020:five_prime_UTR 30.0%
TGATATTCTTTCACCAATCA+CGG + chr8_1:67406706-67406725 None:intergenic 30.0%
TGATTGGTGAAAGAATATCA+TGG - chr8_1:67406706-67406725 Msa1209020:five_prime_UTR 30.0%
! TACAATTTTACCCTTGTGTT+TGG - chr8_1:67406796-67406815 Msa1209020:five_prime_UTR 30.0%
TGTAAAAGAACCAAACACAA+GGG + chr8_1:67406809-67406828 None:intergenic 30.0%
GCAAATGAATGTATTCTCTA+GGG + chr8_1:67405870-67405889 None:intergenic 30.0%
TGCAAATGAATGTATTCTCT+AGG + chr8_1:67405871-67405890 None:intergenic 30.0%
! GTATAGAGAAGCATATTCAA+AGG - chr8_1:67405899-67405918 Msa1209020:three_prime_UTR 30.0%
TTAAAGAACAACCAACAACA+AGG - chr8_1:67406007-67406026 Msa1209020:three_prime_UTR 30.0%
TAAAGAACAACCAACAACAA+GGG - chr8_1:67406008-67406027 Msa1209020:three_prime_UTR 30.0%
TATTGTTTACAGAACAAGAG+AGG - chr8_1:67406316-67406335 Msa1209020:intron 30.0%
! ATGAAGATGTTCAAGTTATG+TGG - chr8_1:67406379-67406398 Msa1209020:intron 30.0%
TCTTTGATGAAGAATTAAGG+TGG - chr8_1:67406625-67406644 Msa1209020:five_prime_UTR 30.0%
GTCAAAATGTTAGTACTTGT+TGG - chr8_1:67406674-67406693 Msa1209020:five_prime_UTR 30.0%
TGATATTCTTTCACCAATCA+CGG + chr8_1:67406706-67406725 None:intergenic 30.0%
TGATTGGTGAAAGAATATCA+TGG - chr8_1:67406706-67406725 Msa1209020:five_prime_UTR 30.0%
! TACAATTTTACCCTTGTGTT+TGG - chr8_1:67406796-67406815 Msa1209020:five_prime_UTR 30.0%
TGTAAAAGAACCAAACACAA+GGG + chr8_1:67406809-67406828 None:intergenic 30.0%
ATCTCTCTCATCAAACCTAA+AGG + chr8_1:67405700-67405719 None:intergenic 35.0%
GTCAATCAACCAAAACCAAA+TGG - chr8_1:67405933-67405952 Msa1209020:three_prime_UTR 35.0%
! TTTTCTTCCAAGAAGCCATT+TGG + chr8_1:67405951-67405970 None:intergenic 35.0%
!!! TTTTTTGAATGGTGTTGCGA+TGG + chr8_1:67405974-67405993 None:intergenic 35.0%
! AAACCTGTTTGAGACTTCTT+AGG + chr8_1:67406036-67406055 None:intergenic 35.0%
TGAAGTTATGTTACACTCTG+TGG + chr8_1:67406433-67406452 None:intergenic 35.0%
CATTCGCTAATTTATGCACT+GGG + chr8_1:67406488-67406507 None:intergenic 35.0%
ACATTCGCTAATTTATGCAC+TGG + chr8_1:67406489-67406508 None:intergenic 35.0%
TTGATGAAGAATTAAGGTGG+CGG - chr8_1:67406628-67406647 Msa1209020:five_prime_UTR 35.0%
ATATTCTTTCACCAATCACG+GGG + chr8_1:67406704-67406723 None:intergenic 35.0%
GATATTCTTTCACCAATCAC+GGG + chr8_1:67406705-67406724 None:intergenic 35.0%
TACTTATTGAACCGTCAAAG+AGG + chr8_1:67406773-67406792 None:intergenic 35.0%
ATCTCTCTCATCAAACCTAA+AGG + chr8_1:67405700-67405719 None:intergenic 35.0%
GTCAATCAACCAAAACCAAA+TGG - chr8_1:67405933-67405952 Msa1209020:three_prime_UTR 35.0%
! TTTTCTTCCAAGAAGCCATT+TGG + chr8_1:67405951-67405970 None:intergenic 35.0%
!!! TTTTTTGAATGGTGTTGCGA+TGG + chr8_1:67405974-67405993 None:intergenic 35.0%
! AAACCTGTTTGAGACTTCTT+AGG + chr8_1:67406036-67406055 None:intergenic 35.0%
TGAAGTTATGTTACACTCTG+TGG + chr8_1:67406433-67406452 None:intergenic 35.0%
CATTCGCTAATTTATGCACT+GGG + chr8_1:67406488-67406507 None:intergenic 35.0%
ACATTCGCTAATTTATGCAC+TGG + chr8_1:67406489-67406508 None:intergenic 35.0%
TTGATGAAGAATTAAGGTGG+CGG - chr8_1:67406628-67406647 Msa1209020:five_prime_UTR 35.0%
ATATTCTTTCACCAATCACG+GGG + chr8_1:67406704-67406723 None:intergenic 35.0%
GATATTCTTTCACCAATCAC+GGG + chr8_1:67406705-67406724 None:intergenic 35.0%
TACTTATTGAACCGTCAAAG+AGG + chr8_1:67406773-67406792 None:intergenic 35.0%
TCTCATCAAACCTAAAGGAC+TGG + chr8_1:67405695-67405714 None:intergenic 40.0%
ACAATCTTAACCTCCTCTCA+CGG - chr8_1:67405755-67405774 Msa1209020:three_prime_UTR 40.0%
CAAACACAAACACAGAGAGA+AGG + chr8_1:67405798-67405817 None:intergenic 40.0%
ACCAAAACCAAATGGCTTCT+TGG - chr8_1:67405941-67405960 Msa1209020:three_prime_UTR 40.0%
!! TCCAAGAAGCCATTTGGTTT+TGG + chr8_1:67405945-67405964 None:intergenic 40.0%
! TTCTTAGGATCCCTTGTTGT+TGG + chr8_1:67406021-67406040 None:intergenic 40.0%
GATCCTAAGAAGTCTCAAAC+AGG - chr8_1:67406030-67406049 Msa1209020:exon 40.0%
! AGAACAAGAGAGGCGTATAA+TGG - chr8_1:67406326-67406345 Msa1209020:intron 40.0%
AAGGTGAAGTAATGGCATGT+GGG - chr8_1:67406355-67406374 Msa1209020:intron 40.0%
TCAAGTTATGTGGTCAATGC+TGG - chr8_1:67406389-67406408 Msa1209020:intron 40.0%
TTATGTGGTCAATGCTGGAT+AGG - chr8_1:67406394-67406413 Msa1209020:intron 40.0%
TTATGCACTGGGACTTCAAA+GGG + chr8_1:67406477-67406496 None:intergenic 40.0%
TTTATGCACTGGGACTTCAA+AGG + chr8_1:67406478-67406497 None:intergenic 40.0%
!!! TTAAGGTGGCGGTTTTAGTT+TGG - chr8_1:67406639-67406658 Msa1209020:five_prime_UTR 40.0%
! GTGTCTGTTTTCCTCTTTGA+CGG - chr8_1:67406759-67406778 Msa1209020:five_prime_UTR 40.0%
TCTCATCAAACCTAAAGGAC+TGG + chr8_1:67405695-67405714 None:intergenic 40.0%
ACAATCTTAACCTCCTCTCA+CGG - chr8_1:67405755-67405774 Msa1209020:three_prime_UTR 40.0%
CAAACACAAACACAGAGAGA+AGG + chr8_1:67405798-67405817 None:intergenic 40.0%
ACCAAAACCAAATGGCTTCT+TGG - chr8_1:67405941-67405960 Msa1209020:three_prime_UTR 40.0%
!! TCCAAGAAGCCATTTGGTTT+TGG + chr8_1:67405945-67405964 None:intergenic 40.0%
! TTCTTAGGATCCCTTGTTGT+TGG + chr8_1:67406021-67406040 None:intergenic 40.0%
GATCCTAAGAAGTCTCAAAC+AGG - chr8_1:67406030-67406049 Msa1209020:exon 40.0%
! AGAACAAGAGAGGCGTATAA+TGG - chr8_1:67406326-67406345 Msa1209020:intron 40.0%
AAGGTGAAGTAATGGCATGT+GGG - chr8_1:67406355-67406374 Msa1209020:intron 40.0%
TCAAGTTATGTGGTCAATGC+TGG - chr8_1:67406389-67406408 Msa1209020:intron 40.0%
TTATGTGGTCAATGCTGGAT+AGG - chr8_1:67406394-67406413 Msa1209020:intron 40.0%
TTATGCACTGGGACTTCAAA+GGG + chr8_1:67406477-67406496 None:intergenic 40.0%
TTTATGCACTGGGACTTCAA+AGG + chr8_1:67406478-67406497 None:intergenic 40.0%
!!! TTAAGGTGGCGGTTTTAGTT+TGG - chr8_1:67406639-67406658 Msa1209020:five_prime_UTR 40.0%
! GTGTCTGTTTTCCTCTTTGA+CGG - chr8_1:67406759-67406778 Msa1209020:five_prime_UTR 40.0%
GTGCTTATGTCCAGTCCTTT+AGG - chr8_1:67405682-67405701 Msa1209020:three_prime_UTR 45.0%
GAGGAGGTTAAGATTGTGGA+AGG + chr8_1:67405752-67405771 None:intergenic 45.0%
GTGAGAGGAGGTTAAGATTG+TGG + chr8_1:67405756-67405775 None:intergenic 45.0%
ACACAGAGAGAAGGAAAGAG+AGG + chr8_1:67405789-67405808 None:intergenic 45.0%
!! AGGCGTATAATGGATCTGCA+AGG - chr8_1:67406336-67406355 Msa1209020:intron 45.0%
GGATCTGCAAGGTGAAGTAA+TGG - chr8_1:67406347-67406366 Msa1209020:intron 45.0%
CAAGGTGAAGTAATGGCATG+TGG - chr8_1:67406354-67406373 Msa1209020:intron 45.0%
TATGTTACACTCTGTGGCTG+TGG + chr8_1:67406427-67406446 None:intergenic 45.0%
GTGCTTATGTCCAGTCCTTT+AGG - chr8_1:67405682-67405701 Msa1209020:three_prime_UTR 45.0%
GAGGAGGTTAAGATTGTGGA+AGG + chr8_1:67405752-67405771 None:intergenic 45.0%
GTGAGAGGAGGTTAAGATTG+TGG + chr8_1:67405756-67405775 None:intergenic 45.0%
ACACAGAGAGAAGGAAAGAG+AGG + chr8_1:67405789-67405808 None:intergenic 45.0%
!! AGGCGTATAATGGATCTGCA+AGG - chr8_1:67406336-67406355 Msa1209020:intron 45.0%
GGATCTGCAAGGTGAAGTAA+TGG - chr8_1:67406347-67406366 Msa1209020:intron 45.0%
CAAGGTGAAGTAATGGCATG+TGG - chr8_1:67406354-67406373 Msa1209020:intron 45.0%
TATGTTACACTCTGTGGCTG+TGG + chr8_1:67406427-67406446 None:intergenic 45.0%
ACACTCTGTGGCTGTGGATT+TGG + chr8_1:67406421-67406440 None:intergenic 50.0%
TTGTTGGGAATCCCCGTGAT+TGG - chr8_1:67406690-67406709 Msa1209020:five_prime_UTR 50.0%
ACACTCTGTGGCTGTGGATT+TGG + chr8_1:67406421-67406440 None:intergenic 50.0%
TTGTTGGGAATCCCCGTGAT+TGG - chr8_1:67406690-67406709 Msa1209020:five_prime_UTR 50.0%
AGAGGAGCAAGATCCGTGAG+AGG + chr8_1:67405771-67405790 None:intergenic 55.0%
AGAGGAGCAAGATCCGTGAG+AGG + chr8_1:67405771-67405790 None:intergenic 55.0%
GGAGCAAGATCCGTGAGAGG+AGG + chr8_1:67405768-67405787 None:intergenic 60.0%
GGAGCAAGATCCGTGAGAGG+AGG + chr8_1:67405768-67405787 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 67405668 67406828 67405668 ID=Msa1209020;Name=Msa1209020
chr8_1 mRNA 67405668 67406828 67405668 ID=Msa1209020-mRNA-1;Parent=Msa1209020;Name=Msa1209020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=284|1|1|1|1|1|2|367|77
chr8_1 exon 67405668 67406168 67405668 ID=Msa1209020-mRNA-1:exon:22309;Parent=Msa1209020-mRNA-1
chr8_1 exon 67406445 67406828 67406445 ID=Msa1209020-mRNA-1:exon:22308;Parent=Msa1209020-mRNA-1
chr8_1 five_prime_UTR 67406545 67406828 67406545 ID=Msa1209020-mRNA-1:five_prime_utr;Parent=Msa1209020-mRNA-1
chr8_1 CDS 67406445 67406544 67406445 ID=Msa1209020-mRNA-1:cds;Parent=Msa1209020-mRNA-1
chr8_1 CDS 67406035 67406168 67406035 ID=Msa1209020-mRNA-1:cds;Parent=Msa1209020-mRNA-1
chr8_1 three_prime_UTR 67405668 67406034 67405668 ID=Msa1209020-mRNA-1:three_prime_utr;Parent=Msa1209020-mRNA-1
Gene Sequence

>Msa1209020

ATGGCTTCTTGGAAGAAAACCATCGCAACACCATTCAAAAAAGCTTGTACTTTCTTTAAAGAACAACCAACAACAAGGGATCCTAAGAAGTCTCAAACAGAACAAGAGAGGCGTATAATGGATCTGCAAGGTGAAGTAATGGCATGTGGGTATGAAGATGTTCAAGTTATGTGGTCAATGCTGGATAGGTCCAAATCCACAGCCACAGAGTGTAACATAACTTCATCAAGTTGA
Protein sequence

>Msa1209020

MASWKKTIATPFKKACTFFKEQPTTRDPKKSQTEQERRIMDLQGEVMACGYEDVQVMWSMLDRSKSTATECNITSSS*