Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1222440 | Msa1222620 | 0.821657 | 3.593498e-53 | -8.615850e-47 |
| Msa1222440 | Msa1222630 | 0.831073 | 2.052257e-55 | -8.615850e-47 |
| Msa1222440 | Msa1334130 | 0.855048 | 8.190402e-62 | -8.615850e-47 |
| Msa1222440 | Msa1334160 | 0.855117 | 7.822280e-62 | -8.615850e-47 |
| Msa1222440 | Msa1334180 | 0.801569 | 8.464270e-49 | -8.615850e-47 |
| Msa1222440 | Msa1334250 | 0.822877 | 1.872682e-53 | -8.615850e-47 |
| Msa1222440 | Msa1337970 | 0.808645 | 2.797506e-50 | -8.615850e-47 |
| Msa1222440 | Msa1406500 | 0.847944 | 8.391991e-60 | -8.615850e-47 |
| Msa1222440 | Msa1416540 | 0.805111 | 1.562800e-49 | -8.615850e-47 |
| Msa1222440 | Msa1416560 | 0.858245 | 9.406868e-63 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGGAAAAGGATCTATTATT+TGG | 0.128131 | 8_1:-86682754 | Msa1222440:CDS |
| TGGAACCTCTTCCATTGATT+TGG | 0.252339 | 8_1:-86682773 | Msa1222440:CDS |
| CTCGGTTTGGTTGATGCTAT+TGG | 0.265550 | 8_1:+86682838 | None:intergenic |
| GCAGAAAATCTTACCTTAGT+TGG | 0.347938 | 8_1:+86682721 | None:intergenic |
| CACAGGGGAAGGGGAAGGTT+TGG | 0.359665 | 8_1:+86682794 | None:intergenic |
| CTCTTCCATTGATTTGGAAA+AGG | 0.400283 | 8_1:-86682767 | Msa1222440:CDS |
| GGAAGAGGTTCCACAGGGGA+AGG | 0.463372 | 8_1:+86682783 | None:intergenic |
| AAGAGGTTCCACAGGGGAAG+GGG | 0.467563 | 8_1:+86682785 | None:intergenic |
| GCAGGATACTACTTAGCAAC+GGG | 0.485513 | 8_1:+86682682 | None:intergenic |
| TACTACTTAGCAACGGGAGC+AGG | 0.490304 | 8_1:+86682688 | None:intergenic |
| GGACGCAACATCTCTCGGTT+TGG | 0.491111 | 8_1:+86682825 | None:intergenic |
| ATCAATGGAAGAGGTTCCAC+AGG | 0.501640 | 8_1:+86682777 | None:intergenic |
| GAAGAGGTTCCACAGGGGAA+GGG | 0.517831 | 8_1:+86682784 | None:intergenic |
| GGTTCCACAGGGGAAGGGGA+AGG | 0.533194 | 8_1:+86682789 | None:intergenic |
| GGTTTGGTTGATGCTATTGG+TGG | 0.540573 | 8_1:+86682841 | None:intergenic |
| TGGACTAAAAGAACCAACTA+AGG | 0.544447 | 8_1:-86682734 | Msa1222440:CDS |
| GTTTGGTTGATGCTATTGGT+GGG | 0.556402 | 8_1:+86682842 | None:intergenic |
| GGTAAGGACGCAACATCTCT+CGG | 0.557645 | 8_1:+86682820 | None:intergenic |
| GGGGAAGGTTTGGACTGGTA+AGG | 0.604771 | 8_1:+86682804 | None:intergenic |
| TGCAGGATACTACTTAGCAA+CGG | 0.610387 | 8_1:+86682681 | None:intergenic |
| GGGAAGGGGAAGGTTTGGAC+TGG | 0.637451 | 8_1:+86682799 | None:intergenic |
| CAAACCTTCCCCTTCCCCTG+TGG | 0.650489 | 8_1:-86682793 | Msa1222440:CDS |
| TCAATGGAAGAGGTTCCACA+GGG | 0.668896 | 8_1:+86682778 | None:intergenic |
| CAATGGAAGAGGTTCCACAG+GGG | 0.794420 | 8_1:+86682779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGATGTTATTGTTATTTAA+TGG | + | chr8_1:86682764-86682783 | None:intergenic | 15.0% |
| !!! | CTAATATAATGTTTTATTAG+TGG | + | chr8_1:86682844-86682863 | None:intergenic | 15.0% |
| !!! | GGTGTTATTGTAATTTATTA+GGG | + | chr8_1:86682823-86682842 | None:intergenic | 20.0% |
| !!! | TGGTGTTATTGTAATTTATT+AGG | + | chr8_1:86682824-86682843 | None:intergenic | 20.0% |
| !! | TGTTGGTATAATAAATAACT+AGG | + | chr8_1:86682880-86682899 | None:intergenic | 20.0% |
| ! | TATTGTAATTTATTAGGGAG+AGG | + | chr8_1:86682818-86682837 | None:intergenic | 25.0% |
| ! | TGGTATAATAAATAACTAGG+AGG | + | chr8_1:86682877-86682896 | None:intergenic | 25.0% |
| ! | TATTGCTATAGCAAAATTGA+AGG | + | chr8_1:86683083-86683102 | None:intergenic | 25.0% |
| ! | TTGGAAAAGGATCTATTATT+TGG | - | chr8_1:86683202-86683221 | Msa1222440:intron | 25.0% |
| GTTACTGCATTATTCGTAAA+GGG | + | chr8_1:86682921-86682940 | None:intergenic | 30.0% | |
| TGTTACTGCATTATTCGTAA+AGG | + | chr8_1:86682922-86682941 | None:intergenic | 30.0% | |
| AGGAGGAATTTAATCTTCTT+TGG | + | chr8_1:86682988-86683007 | None:intergenic | 30.0% | |
| ! | TAGATCCTTTTCCAAATCAA+TGG | + | chr8_1:86683197-86683216 | None:intergenic | 30.0% |
| GTAATTTATTAGGGAGAGGA+GGG | + | chr8_1:86682814-86682833 | None:intergenic | 35.0% | |
| TGTAATTTATTAGGGAGAGG+AGG | + | chr8_1:86682815-86682834 | None:intergenic | 35.0% | |
| AAGTTGTGACCAAGAAGATT+AGG | - | chr8_1:86682941-86682960 | Msa1222440:intron | 35.0% | |
| TTAGTGCATCCTAATCTTCT+TGG | + | chr8_1:86682953-86682972 | None:intergenic | 35.0% | |
| AGAAGATTAGGATGCACTAA+CGG | - | chr8_1:86682953-86682972 | Msa1222440:intron | 35.0% | |
| CAATATACCTCAAAATGGTG+AGG | + | chr8_1:86683059-86683078 | None:intergenic | 35.0% | |
| !!! | CTCACCATTTTGAGGTATAT+TGG | - | chr8_1:86683057-86683076 | Msa1222440:intron | 35.0% |
| AAGGCCAATATACCTCAAAA+TGG | + | chr8_1:86683064-86683083 | None:intergenic | 35.0% | |
| ! | CTTTTCCAAATCAATGGAAG+AGG | + | chr8_1:86683191-86683210 | None:intergenic | 35.0% |
| !! | CTCTTCCATTGATTTGGAAA+AGG | - | chr8_1:86683189-86683208 | Msa1222440:intron | 35.0% |
| TGGACTAAAAGAACCAACTA+AGG | - | chr8_1:86683222-86683241 | Msa1222440:intron | 35.0% | |
| GCAGAAAATCTTACCTTAGT+TGG | + | chr8_1:86683238-86683257 | None:intergenic | 35.0% | |
| ATTATTCGTAAAGGGAGAGG+GGG | + | chr8_1:86682913-86682932 | None:intergenic | 40.0% | |
| CATTATTCGTAAAGGGAGAG+GGG | + | chr8_1:86682914-86682933 | None:intergenic | 40.0% | |
| GCATTATTCGTAAAGGGAGA+GGG | + | chr8_1:86682915-86682934 | None:intergenic | 40.0% | |
| TGCATTATTCGTAAAGGGAG+AGG | + | chr8_1:86682916-86682935 | None:intergenic | 40.0% | |
| GAAGATTAGGATGCACTAAC+GGG | - | chr8_1:86682954-86682973 | Msa1222440:intron | 40.0% | |
| ! | AGAGTAACCTCACCATTTTG+AGG | - | chr8_1:86683049-86683068 | Msa1222440:intron | 40.0% |
| ! | GTTTGGTTGATGCTATTGGT+GGG | + | chr8_1:86683117-86683136 | None:intergenic | 40.0% |
| ! | TGGAACCTCTTCCATTGATT+TGG | - | chr8_1:86683183-86683202 | Msa1222440:intron | 40.0% |
| ! | GAGGGGGGATATTTTCTTGT+TGG | + | chr8_1:86682897-86682916 | None:intergenic | 45.0% |
| TTATTCGTAAAGGGAGAGGG+GGG | + | chr8_1:86682912-86682931 | None:intergenic | 45.0% | |
| ATTAAATTCCTCCTCCTCCC+AGG | - | chr8_1:86682994-86683013 | Msa1222440:intron | 45.0% | |
| TTAAATTCCTCCTCCTCCCA+GGG | - | chr8_1:86682995-86683014 | Msa1222440:intron | 45.0% | |
| ! | GGTTTGGTTGATGCTATTGG+TGG | + | chr8_1:86683118-86683137 | None:intergenic | 45.0% |
| !! | CTCGGTTTGGTTGATGCTAT+TGG | + | chr8_1:86683121-86683140 | None:intergenic | 45.0% |
| TCAATGGAAGAGGTTCCACA+GGG | + | chr8_1:86683181-86683200 | None:intergenic | 45.0% | |
| ATCAATGGAAGAGGTTCCAC+AGG | + | chr8_1:86683182-86683201 | None:intergenic | 45.0% | |
| TCCCAGGGTAAAGTTGGACA+GGG | - | chr8_1:86683010-86683029 | Msa1222440:intron | 50.0% | |
| ACCCTGTCCAACTTTACCCT+GGG | + | chr8_1:86683014-86683033 | None:intergenic | 50.0% | |
| GGTAAGGACGCAACATCTCT+CGG | + | chr8_1:86683139-86683158 | None:intergenic | 50.0% | |
| CAATGGAAGAGGTTCCACAG+GGG | + | chr8_1:86683180-86683199 | None:intergenic | 50.0% | |
| AACTTTACCCTGGGAGGAGG+AGG | + | chr8_1:86683005-86683024 | None:intergenic | 55.0% | |
| TCCTCCTCCCAGGGTAAAGT+TGG | - | chr8_1:86683004-86683023 | Msa1222440:intron | 55.0% | |
| TCCAACTTTACCCTGGGAGG+AGG | + | chr8_1:86683008-86683027 | None:intergenic | 55.0% | |
| CTGTCCAACTTTACCCTGGG+AGG | + | chr8_1:86683011-86683030 | None:intergenic | 55.0% | |
| CTCCCAGGGTAAAGTTGGAC+AGG | - | chr8_1:86683009-86683028 | Msa1222440:intron | 55.0% | |
| CACCCTGTCCAACTTTACCC+TGG | + | chr8_1:86683015-86683034 | None:intergenic | 55.0% | |
| GGACGCAACATCTCTCGGTT+TGG | + | chr8_1:86683134-86683153 | None:intergenic | 55.0% | |
| !! | GGGGAAGGTTTGGACTGGTA+AGG | + | chr8_1:86683155-86683174 | None:intergenic | 55.0% |
| AAGAGGTTCCACAGGGGAAG+GGG | + | chr8_1:86683174-86683193 | None:intergenic | 55.0% | |
| GAAGAGGTTCCACAGGGGAA+GGG | + | chr8_1:86683175-86683194 | None:intergenic | 55.0% | |
| GGGAAGGGGAAGGTTTGGAC+TGG | + | chr8_1:86683160-86683179 | None:intergenic | 60.0% | |
| CACAGGGGAAGGGGAAGGTT+TGG | + | chr8_1:86683165-86683184 | None:intergenic | 60.0% | |
| CAAACCTTCCCCTTCCCCTG+TGG | - | chr8_1:86683163-86683182 | Msa1222440:intron | 60.0% | |
| GGAAGAGGTTCCACAGGGGA+AGG | + | chr8_1:86683176-86683195 | None:intergenic | 60.0% | |
| GGTTCCACAGGGGAAGGGGA+AGG | + | chr8_1:86683170-86683189 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 86682690 | 86683288 | 86682690 | ID=Msa1222440;Name=Msa1222440 |
| chr8_1 | mRNA | 86682690 | 86683288 | 86682690 | ID=Msa1222440-mRNA-1;Parent=Msa1222440;Name=Msa1222440-mRNA-1;_AED=0.50;_eAED=1.00;_QI=0|0|0|0.5|0|0|2|0|65 |
| chr8_1 | exon | 86683282 | 86683288 | 86683282 | ID=Msa1222440-mRNA-1:exon:31267;Parent=Msa1222440-mRNA-1 |
| chr8_1 | exon | 86682690 | 86682880 | 86682690 | ID=Msa1222440-mRNA-1:exon:31266;Parent=Msa1222440-mRNA-1 |
| chr8_1 | CDS | 86683282 | 86683288 | 86683282 | ID=Msa1222440-mRNA-1:cds;Parent=Msa1222440-mRNA-1 |
| chr8_1 | CDS | 86682690 | 86682880 | 86682690 | ID=Msa1222440-mRNA-1:cds;Parent=Msa1222440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
