AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1231700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1231700 MtrunA17_Chr8g0340641 94.211 380 21 1 1 380 1 379 0.0 741
Msa1231700 MtrunA17_Chr7g0242551 25.271 277 185 7 83 353 71 331 4.75e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1231700 AT1G79760.2 34.718 337 139 13 8 339 17 277 9.03e-48 165
Msa1231700 AT1G79760.1 34.535 333 138 13 11 339 1 257 2.63e-47 163
Msa1231700 AT2G38500.1 27.000 300 182 9 29 324 28 294 9.45e-18 84.0
Msa1231700 AT5G05600.1 23.843 281 195 7 70 347 63 327 3.96e-13 70.5

Find 118 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAATTTCTCACCGAGTTTC+TGG 0.068021 8_2:-7977012 Msa1231700:CDS
CATATTTGTTTGAAGTTTCT+TGG 0.181857 8_2:+7974266 None:intergenic
TCTAATATTTATCTTTAAAA+AGG 0.185759 8_2:-7974178 Msa1231700:CDS
TTTCGCTGTTCTACGATATT+AGG 0.205543 8_2:-7973815 Msa1231700:three_prime_UTR
TTTCGTCATTGCTCTGTTTC+GGG 0.228612 8_2:+7976943 None:intergenic
GAAGGTGGCTGAGAAAATTT+TGG 0.236901 8_2:-7974946 Msa1231700:CDS
AGTTGAAATTGGAGGGAATT+TGG 0.246627 8_2:-7976608 Msa1231700:CDS
ATTGGAAAGGCAATATTTAA+AGG 0.263472 8_2:-7974339 Msa1231700:CDS
ACTGGAAAGTCTATAGTTAA+AGG 0.265271 8_2:-7973863 Msa1231700:three_prime_UTR
TCATGCTTGCTCGGATGATT+TGG 0.283452 8_2:+7973631 None:intergenic
CGGAAATCAACGGTGGTTTC+CGG 0.284712 8_2:+7977089 None:intergenic
AGTTGAATGAAGAATTTATT+TGG 0.285478 8_2:-7976653 Msa1231700:CDS
ATTACTAGGGCACCTTGTTC+AGG 0.285696 8_2:+7974660 None:intergenic
GTTTCGTCATTGCTCTGTTT+CGG 0.288438 8_2:+7976942 None:intergenic
TAATGATCGCATTGTGAAAA+TGG 0.290296 8_2:+7977133 None:intergenic
TGAAGAGTTGAAGTTGAAAT+TGG 0.298562 8_2:-7976619 Msa1231700:CDS
AATTCGAAATACCTAAAATT+AGG 0.305210 8_2:+7976573 None:intergenic
GGGCATTACAAGGATGTAAT+TGG 0.306906 8_2:-7974357 Msa1231700:CDS
GATCTCCAGCAGTGATTACT+AGG 0.312300 8_2:+7974646 None:intergenic
TTGCTGCACATACTGAGATT+TGG 0.313362 8_2:-7973902 Msa1231700:three_prime_UTR
TGCTGCACATACTGAGATTT+GGG 0.320728 8_2:-7973901 Msa1231700:three_prime_UTR
CATTAGTTTCAAAGGTTTGA+TGG 0.330434 8_2:+7973702 None:intergenic
CAAGAAACTTCAAACAAATA+TGG 0.338970 8_2:-7974265 Msa1231700:CDS
CAATACCAACTTCCTTGATT+AGG 0.349218 8_2:+7973927 None:intergenic
AGCATCATCAAGATATTAAT+AGG 0.354575 8_2:-7974830 Msa1231700:CDS
ATGTGATTAGAATGCTGATT+AGG 0.357036 8_2:-7974776 Msa1231700:CDS
GCAAGTGATGGTGATATAAT+TGG 0.358719 8_2:-7974891 Msa1231700:CDS
GCTTGTTGGCCAAGTGAAAT+AGG 0.379497 8_2:+7974237 None:intergenic
TGTGATTAGAATGCTGATTA+GGG 0.384993 8_2:-7974775 Msa1231700:CDS
GGATCTCATTGACGTTGTTT+CGG 0.388813 8_2:-7976874 Msa1231700:CDS
ACTTGGCCAACAAGCCATCT+TGG 0.392357 8_2:-7974229 Msa1231700:CDS
TTGTCCGGTAATACTAGATC+TGG 0.395672 8_2:+7976972 None:intergenic
GCTGCTGCAGTGGAGGTTGC+CGG 0.395912 8_2:-7976789 Msa1231700:CDS
TTATCATATAATTGTTTAAG+TGG 0.400439 8_2:+7974076 None:intergenic
TTGAAATTGCAGCAAGAAGA+TGG 0.406949 8_2:-7974991 Msa1231700:intron
TCGGAATCTATAGCTAGGTT+TGG 0.407064 8_2:-7976855 Msa1231700:CDS
TCTATAACTTACTACGTGGT+TGG 0.416323 8_2:+7973967 None:intergenic
ATATTAATAGGAATGATCAT+TGG 0.434233 8_2:-7974818 Msa1231700:CDS
TGACTAGTTTATCCTAATCA+AGG 0.437981 8_2:-7973939 Msa1231700:three_prime_UTR
AGTTCCAGATCTAGTATTAC+CGG 0.444942 8_2:-7976976 Msa1231700:CDS
ACACATGCAAACATAAAGAA+TGG 0.451305 8_2:+7974739 None:intergenic
GGAATATTTACGGTGCCGTC+GGG 0.453618 8_2:-7976765 Msa1231700:CDS
TGATGAAGTAGAAGAAGAAG+AGG 0.462264 8_2:-7976685 Msa1231700:CDS
GTAGTCTTTGAGATTGTTGT+GGG 0.462510 8_2:+7973996 None:intergenic
AAGAGAAGACAACATTTCAA+TGG 0.463032 8_2:-7974313 Msa1231700:CDS
GTTCAAAATTACAGCCAAGA+TGG 0.463235 8_2:+7974215 None:intergenic
GGTTGCCGGAGGAATATTTA+CGG 0.463294 8_2:-7976775 Msa1231700:CDS
TTTGAAAGCAACGATGAGAA+TGG 0.474113 8_2:+7977187 None:intergenic
TTACAGCCAAGATGGCTTGT+TGG 0.474725 8_2:+7974223 None:intergenic
TATTAATAGGAATGATCATT+GGG 0.475240 8_2:-7974817 Msa1231700:CDS
TGATAAATTAAATGAGTACA+TGG 0.481095 8_2:+7973741 None:intergenic
ATATTAGAACGTGGTACATA+AGG 0.481548 8_2:+7974193 None:intergenic
ACTGCAGCAGCCACAATTCG+CGG 0.481741 8_2:+7976801 None:intergenic
TGATAGTGTGTTTGTTGAAT+CGG 0.483877 8_2:+7977258 None:intergenic
ATCAACATTGTCCTCTGTAA+AGG 0.486423 8_2:-7977347 Msa1231700:five_prime_UTR
ATTGGTCATAGAAATGAAGT+TGG 0.490467 8_2:-7974873 Msa1231700:CDS
GGCTATAGGCATTGAGTGGA+GGG 0.491223 8_2:-7974377 Msa1231700:intron
GAGATCGCCGGAGAAACCTC+GGG 0.491548 8_2:-7976724 Msa1231700:CDS
TTGGAGGGAATTTGGCCTGT+TGG 0.491974 8_2:-7976600 Msa1231700:CDS
ATGTTTACTCAAAGAAAAGT+TGG 0.492603 8_2:-7974695 Msa1231700:CDS
CACATGCAAACATAAAGAAT+GGG 0.494216 8_2:+7974740 None:intergenic
ATTTATTTCATTAGTTTCAA+AGG 0.496537 8_2:+7973694 None:intergenic
TTACTAGGGCACCTTGTTCA+GGG 0.500069 8_2:+7974661 None:intergenic
GTGGTTTCCGGCATGGTTTG+CGG 0.500737 8_2:+7977101 None:intergenic
CGACCGGAGGCTACCGGTGA+TGG 0.501289 8_2:+7977056 None:intergenic
TTATGTTTGCATGTGTGTGA+TGG 0.501762 8_2:-7974732 Msa1231700:CDS
TGCTTTCAGCTTATTAACCA+TGG 0.504255 8_2:-7976831 Msa1231700:CDS
TTTATCCTAATCAAGGAAGT+TGG 0.507700 8_2:-7973932 Msa1231700:three_prime_UTR
TTGTCTTTCTGCCCTGAACA+AGG 0.507702 8_2:-7974672 Msa1231700:CDS
TCAAAACCCCGAGGTTTCTC+CGG 0.510007 8_2:+7976717 None:intergenic
TGGAATATCGCCGCGAATTG+TGG 0.513817 8_2:-7976811 Msa1231700:CDS
TGTAGTCTTTGAGATTGTTG+TGG 0.514107 8_2:+7973995 None:intergenic
CCGTGCACCGCCGCCATCAC+CGG 0.514670 8_2:-7977069 Msa1231700:CDS
CCGGTGATGGCGGCGGTGCA+CGG 0.517525 8_2:+7977069 None:intergenic
ATCAACGGTGGTTTCCGGCA+TGG 0.522750 8_2:+7977094 None:intergenic
GCGAATTGTGGCTGCTGCAG+TGG 0.524178 8_2:-7976799 Msa1231700:CDS
GGGAACTATAAGCAAATGAC+TGG 0.532695 8_2:-7973881 Msa1231700:three_prime_UTR
CAATTCGCGGCGATATTCCA+TGG 0.533113 8_2:+7976814 None:intergenic
TTGGTCATAGAAATGAAGTT+GGG 0.535395 8_2:-7974872 Msa1231700:CDS
GAGTTGAAGTTGAAATTGGA+GGG 0.535578 8_2:-7976615 Msa1231700:CDS
GGTGCCCTAGTAATCACTGC+TGG 0.539493 8_2:-7974651 Msa1231700:CDS
TGTTTCGGGAAAATCTTGTC+CGG 0.539842 8_2:+7976957 None:intergenic
TTGTTTCGGAATCTATAGCT+AGG 0.540237 8_2:-7976860 Msa1231700:CDS
CTAAAATTAGGATATCCAAC+AGG 0.541512 8_2:+7976585 None:intergenic
AGAGTTGAAGTTGAAATTGG+AGG 0.544255 8_2:-7976616 Msa1231700:CDS
CGAGATCGCCGGAGAAACCT+CGG 0.547961 8_2:-7976725 Msa1231700:CDS
AGGAATATTTACGGTGCCGT+CGG 0.549924 8_2:-7976766 Msa1231700:CDS
GAGGCTACCGGTGATGGCGG+CGG 0.552842 8_2:+7977062 None:intergenic
CACTGCTGGAGATCAAATCC+AGG 0.554273 8_2:-7974637 Msa1231700:intron
TGGCTATAGGCATTGAGTGG+AGG 0.560050 8_2:-7974378 Msa1231700:intron
AGTGAAACGAAATCGATCTT+CGG 0.565003 8_2:+7976915 None:intergenic
CGGCACCGTAAATATTCCTC+CGG 0.565215 8_2:+7976770 None:intergenic
TCTACAATCACGTGTGGAGA+AGG 0.568223 8_2:-7974964 Msa1231700:CDS
ATGGAAAGACCTATTTCACT+TGG 0.570120 8_2:-7974246 Msa1231700:CDS
GATTCTGTTCACATTTCAAG+AGG 0.573329 8_2:-7973610 Msa1231700:three_prime_UTR
TGAACAGAATCATGCTTGCT+CGG 0.578747 8_2:+7973622 None:intergenic
ATTGAGTGGAGGGCATTACA+AGG 0.581852 8_2:-7974367 Msa1231700:CDS
TTACAAGGATGTAATTGGAA+AGG 0.584583 8_2:-7974352 Msa1231700:CDS
AATTGTGGCTGCTGCAGTGG+AGG 0.593450 8_2:-7976796 Msa1231700:CDS
ATTAACACCGCAAACCATGC+CGG 0.596152 8_2:-7977108 Msa1231700:CDS
AGATGCTGTGACGAGATCGC+CGG 0.604287 8_2:-7976736 Msa1231700:CDS
GCTGCAGTGGAGGTTGCCGG+AGG 0.607964 8_2:-7976786 Msa1231700:CDS
CGGCGGTGCACGGAAATCAA+CGG 0.614742 8_2:+7977079 None:intergenic
TGTTACCGTTGATCTACGAC+CGG 0.620887 8_2:+7977040 None:intergenic
ATCTCCAGCAGTGATTACTA+GGG 0.624399 8_2:+7974647 None:intergenic
ACAATCACGTGTGGAGAAGG+TGG 0.625775 8_2:-7974961 Msa1231700:CDS
GCTTGGCTATAGGCATTGAG+TGG 0.637199 8_2:-7974381 Msa1231700:intron
AAGCGAAGTTTCCAGAAACT+CGG 0.638861 8_2:+7977001 None:intergenic
AGCCTCCGGTCGTAGATCAA+CGG 0.648849 8_2:-7977045 Msa1231700:CDS
GGAAACTCTACAATCACGTG+TGG 0.665148 8_2:-7974970 Msa1231700:CDS
GAAACGAAATCGATCTTCGG+CGG 0.683182 8_2:+7976918 None:intergenic
TAAAGATAAATATTAGAACG+TGG 0.683558 8_2:+7974184 None:intergenic
ACAGCATCTCTCATTCCCGA+CGG 0.714149 8_2:+7976750 None:intergenic
CGGTGCACGGAAATCAACGG+TGG 0.737046 8_2:+7977082 None:intergenic
GTGATTAGAATGCTGATTAG+GGG 0.740101 8_2:-7974774 Msa1231700:CDS
TGATACTCTTCAAAACCCCG+AGG 0.741463 8_2:+7976708 None:intergenic
AGATCGCCGGAGAAACCTCG+GGG 0.744747 8_2:-7976723 Msa1231700:CDS
TTTATCTATAACTTACTACG+TGG 0.746580 8_2:+7973963 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTATAAAAAAAAAAAAAAC+CGG - chr8_2:7974723-7974742 Msa1231700:CDS 10.0%
!!! TCTAATATTTATCTTTAAAA+AGG - chr8_2:7976766-7976785 Msa1231700:CDS 10.0%
!!! ATGTTTTAAAGTAGAAAAAT+TGG - chr8_2:7975557-7975576 Msa1231700:intron 15.0%
!!! ATTTTTTTCTTCTTTTTTTG+AGG + chr8_2:7975826-7975845 None:intergenic 15.0%
!! AAAAAGAAGAAAAAAATAGT+AGG - chr8_2:7975829-7975848 Msa1231700:intron 15.0%
!!! GTTTATTTTGTATATATTGA+TGG - chr8_2:7975872-7975891 Msa1231700:intron 15.0%
!! TTATCATATAATTGTTTAAG+TGG + chr8_2:7976871-7976890 None:intergenic 15.0%
!!! ATTTATTTCATTAGTTTCAA+AGG + chr8_2:7977253-7977272 None:intergenic 15.0%
!! AGTTGAATGAAGAATTTATT+TGG - chr8_2:7974291-7974310 Msa1231700:CDS 20.0%
!! AATTCGAAATACCTAAAATT+AGG + chr8_2:7974374-7974393 None:intergenic 20.0%
!! TTGAACTTCAAAGTTTATTA+AGG + chr8_2:7974625-7974644 None:intergenic 20.0%
!!! TACGATATATTTTAACAATC+CGG + chr8_2:7974745-7974764 None:intergenic 20.0%
!!! AATCATAAGTAGTTTGTATT+AGG + chr8_2:7974844-7974863 None:intergenic 20.0%
!!! TATATAATATTGGGTTTTGA+CGG - chr8_2:7974970-7974989 Msa1231700:CDS 20.0%
!!! GTGAAGAATATGTATTTTAT+AGG - chr8_2:7975353-7975372 Msa1231700:intron 20.0%
!! AAGAAAAAAATAGTAGGTAT+TGG - chr8_2:7975835-7975854 Msa1231700:intron 20.0%
!! ATATTAATAGGAATGATCAT+TGG - chr8_2:7976126-7976145 Msa1231700:intron 20.0%
!! TATTAATAGGAATGATCATT+GGG - chr8_2:7976127-7976146 Msa1231700:intron 20.0%
!! TAAAGATAAATATTAGAACG+TGG + chr8_2:7976763-7976782 None:intergenic 20.0%
!! TGATAAATTAAATGAGTACA+TGG + chr8_2:7977206-7977225 None:intergenic 20.0%
! AATCAAAAAGCAAAACAGAA+AGG - chr8_2:7973630-7973649 Msa1231700:three_prime_UTR 25.0%
! TGAATTTCAAGGTTTCATTA+AGG + chr8_2:7974686-7974705 None:intergenic 25.0%
!! TTTAGATGAGTTGAATTTCA+AGG + chr8_2:7974697-7974716 None:intergenic 25.0%
!!! ATATAATATTGGGTTTTGAC+GGG - chr8_2:7974971-7974990 Msa1231700:CDS 25.0%
! CTCAAAATCTTTATTATGCA+AGG + chr8_2:7975515-7975534 None:intergenic 25.0%
!! CTTGCATAATAAAGATTTTG+AGG - chr8_2:7975513-7975532 Msa1231700:intron 25.0%
!! AACAATCTATCTTTACGTTT+TGG - chr8_2:7975685-7975704 Msa1231700:intron 25.0%
! ATGTACGTATCTAAATTTGT+CGG + chr8_2:7975749-7975768 None:intergenic 25.0%
! AAAAAGAAATTAGCAAGTGA+TGG - chr8_2:7976041-7976060 Msa1231700:intron 25.0%
! AGCATCATCAAGATATTAAT+AGG - chr8_2:7976114-7976133 Msa1231700:intron 25.0%
! ATGTTTACTCAAAGAAAAGT+TGG - chr8_2:7976249-7976268 Msa1231700:intron 25.0%
! AGCAAAAATGTAAAATAACC+TGG + chr8_2:7976328-7976347 None:intergenic 25.0%
!! TGAAGGTATAGTCAAAATAT+TGG + chr8_2:7976475-7976494 None:intergenic 25.0%
! ATTGGAAAGGCAATATTTAA+AGG - chr8_2:7976605-7976624 Msa1231700:CDS 25.0%
!! CATATTTGTTTGAAGTTTCT+TGG + chr8_2:7976681-7976700 None:intergenic 25.0%
! CAAGAAACTTCAAACAAATA+TGG - chr8_2:7976679-7976698 Msa1231700:CDS 25.0%
!! AGATTATGCATAAGATTTTG+AGG + chr8_2:7976920-7976939 None:intergenic 25.0%
! TTTATCTATAACTTACTACG+TGG + chr8_2:7976984-7977003 None:intergenic 25.0%
! ATTTGGACTTCATTAAAAAG+TGG + chr8_2:7977299-7977318 None:intergenic 25.0%
! TGATAGTGTGTTTGTTGAAT+CGG + chr8_2:7973689-7973708 None:intergenic 30.0%
TAATGATCGCATTGTGAAAA+TGG + chr8_2:7973814-7973833 None:intergenic 30.0%
TGAAGAGTTGAAGTTGAAAT+TGG - chr8_2:7974325-7974344 Msa1231700:CDS 30.0%
CTAAAATTAGGATATCCAAC+AGG + chr8_2:7974362-7974381 None:intergenic 30.0%
! CTGTTGGATATCCTAATTTT+AGG - chr8_2:7974360-7974379 Msa1231700:CDS 30.0%
TGATACTCGTAAATCAAACT+CGG + chr8_2:7974542-7974561 None:intergenic 30.0%
CGAATGATATGTAGATATCT+CGG - chr8_2:7974568-7974587 Msa1231700:intron 30.0%
TACAATTACACACACTCATT+TGG - chr8_2:7975076-7975095 Msa1231700:intron 30.0%
ACAATTACACACACTCATTT+GGG - chr8_2:7975077-7975096 Msa1231700:intron 30.0%
! AGTTTTTATCAAGATTGACG+TGG - chr8_2:7975229-7975248 Msa1231700:intron 30.0%
! GTTTTTATCAAGATTGACGT+GGG - chr8_2:7975230-7975249 Msa1231700:intron 30.0%
! TAAGAGTGTGAGATATAAAG+TGG - chr8_2:7975295-7975314 Msa1231700:intron 30.0%
AATTATGTACCTTGCATTTG+TGG - chr8_2:7975331-7975350 Msa1231700:intron 30.0%
TGTACGTATCTAAATTTGTC+GGG + chr8_2:7975748-7975767 None:intergenic 30.0%
ATTGGTCATAGAAATGAAGT+TGG - chr8_2:7976071-7976090 Msa1231700:intron 30.0%
TTGGTCATAGAAATGAAGTT+GGG - chr8_2:7976072-7976091 Msa1231700:intron 30.0%
! ATGTGATTAGAATGCTGATT+AGG - chr8_2:7976168-7976187 Msa1231700:intron 30.0%
! TGTGATTAGAATGCTGATTA+GGG - chr8_2:7976169-7976188 Msa1231700:intron 30.0%
CACATGCAAACATAAAGAAT+GGG + chr8_2:7976207-7976226 None:intergenic 30.0%
ACACATGCAAACATAAAGAA+TGG + chr8_2:7976208-7976227 None:intergenic 30.0%
TCAAACTCGTTAAATGAGAT+GGG + chr8_2:7976539-7976558 None:intergenic 30.0%
ATCAAACTCGTTAAATGAGA+TGG + chr8_2:7976540-7976559 None:intergenic 30.0%
TTAACGAGTTTGATATTGCT+TGG - chr8_2:7976546-7976565 Msa1231700:intron 30.0%
TTACAAGGATGTAATTGGAA+AGG - chr8_2:7976592-7976611 Msa1231700:CDS 30.0%
AAGAGAAGACAACATTTCAA+TGG - chr8_2:7976631-7976650 Msa1231700:CDS 30.0%
ATATTAGAACGTGGTACATA+AGG + chr8_2:7976754-7976773 None:intergenic 30.0%
!! TGACTAGTTTATCCTAATCA+AGG - chr8_2:7977005-7977024 Msa1231700:CDS 30.0%
TTTATCCTAATCAAGGAAGT+TGG - chr8_2:7977012-7977031 Msa1231700:CDS 30.0%
ACTGGAAAGTCTATAGTTAA+AGG - chr8_2:7977081-7977100 Msa1231700:CDS 30.0%
! CATTAGTTTCAAAGGTTTGA+TGG + chr8_2:7977245-7977264 None:intergenic 30.0%
TTCATTAAAAAGTGGACAGT+GGG + chr8_2:7977291-7977310 None:intergenic 30.0%
TTTGAAAGCAACGATGAGAA+TGG + chr8_2:7973760-7973779 None:intergenic 35.0%
AGTTCCAGATCTAGTATTAC+CGG - chr8_2:7973968-7973987 Msa1231700:three_prime_UTR 35.0%
AGTGAAACGAAATCGATCTT+CGG + chr8_2:7974032-7974051 None:intergenic 35.0%
TTGTTTCGGAATCTATAGCT+AGG - chr8_2:7974084-7974103 Msa1231700:three_prime_UTR 35.0%
TGCTTTCAGCTTATTAACCA+TGG - chr8_2:7974113-7974132 Msa1231700:three_prime_UTR 35.0%
TGATGAAGTAGAAGAAGAAG+AGG - chr8_2:7974259-7974278 Msa1231700:CDS 35.0%
AGAGTTGAAGTTGAAATTGG+AGG - chr8_2:7974328-7974347 Msa1231700:CDS 35.0%
GAGTTGAAGTTGAAATTGGA+GGG - chr8_2:7974329-7974348 Msa1231700:CDS 35.0%
AGTTGAAATTGGAGGGAATT+TGG - chr8_2:7974336-7974355 Msa1231700:CDS 35.0%
TTAACTTACGTCCAAAGTTG+TGG - chr8_2:7974507-7974526 Msa1231700:intron 35.0%
TTCAAGGTTTCATTAAGGTC+AGG + chr8_2:7974681-7974700 None:intergenic 35.0%
CTTACGTGATCAAGAAGAAT+TGG - chr8_2:7975194-7975213 Msa1231700:intron 35.0%
TATTCTTCACCACAAATGCA+AGG + chr8_2:7975343-7975362 None:intergenic 35.0%
!!! GTATTTTATAGGCGCTGATA+AGG - chr8_2:7975364-7975383 Msa1231700:intron 35.0%
TTGAAATTGCAGCAAGAAGA+TGG - chr8_2:7975953-7975972 Msa1231700:intron 35.0%
! GCAAGTGATGGTGATATAAT+TGG - chr8_2:7976053-7976072 Msa1231700:intron 35.0%
! GTGATTAGAATGCTGATTAG+GGG - chr8_2:7976170-7976189 Msa1231700:intron 35.0%
TTATGTTTGCATGTGTGTGA+TGG - chr8_2:7976212-7976231 Msa1231700:intron 35.0%
AAAAAACTCCAACTCCACTT+TGG - chr8_2:7976387-7976406 Msa1231700:intron 35.0%
ATTTCAAACCAAAGTGGAGT+TGG + chr8_2:7976398-7976417 None:intergenic 35.0%
GTTCGTATTTCAAACCAAAG+TGG + chr8_2:7976404-7976423 None:intergenic 35.0%
TTGCTGTCATGAAAGATTGA+AGG + chr8_2:7976492-7976511 None:intergenic 35.0%
GTTTGATATTGCTTGGCTAT+AGG - chr8_2:7976553-7976572 Msa1231700:intron 35.0%
! TGAAGTTTCTTGGCATTTTG+AGG + chr8_2:7976671-7976690 None:intergenic 35.0%
ATGGAAAGACCTATTTCACT+TGG - chr8_2:7976698-7976717 Msa1231700:CDS 35.0%
GTTCAAAATTACAGCCAAGA+TGG + chr8_2:7976732-7976751 None:intergenic 35.0%
!!! TAAGATTTTGAGGTGCAAAC+AGG + chr8_2:7976910-7976929 None:intergenic 35.0%
!! GTAGTCTTTGAGATTGTTGT+GGG + chr8_2:7976951-7976970 None:intergenic 35.0%
!! TGTAGTCTTTGAGATTGTTG+TGG + chr8_2:7976952-7976971 None:intergenic 35.0%
TCTATAACTTACTACGTGGT+TGG + chr8_2:7976980-7976999 None:intergenic 35.0%
CAATACCAACTTCCTTGATT+AGG + chr8_2:7977020-7977039 None:intergenic 35.0%
TTTCGCTGTTCTACGATATT+AGG - chr8_2:7977129-7977148 Msa1231700:exon 35.0%
CTTCATTAAAAAGTGGACAG+TGG + chr8_2:7977292-7977311 None:intergenic 35.0%
GATTCTGTTCACATTTCAAG+AGG - chr8_2:7977334-7977353 Msa1231700:five_prime_UTR 35.0%
! TGCTTCGTTTTCCTTTACAG+AGG + chr8_2:7973611-7973630 None:intergenic 40.0%
AAGCGAAGTTTCCAGAAACT+CGG + chr8_2:7973946-7973965 None:intergenic 40.0%
TTGTCCGGTAATACTAGATC+TGG + chr8_2:7973975-7973994 None:intergenic 40.0%
TGTTTCGGGAAAATCTTGTC+CGG + chr8_2:7973990-7974009 None:intergenic 40.0%
TTTCGTCATTGCTCTGTTTC+GGG + chr8_2:7974004-7974023 None:intergenic 40.0%
GTTTCGTCATTGCTCTGTTT+CGG + chr8_2:7974005-7974024 None:intergenic 40.0%
! ACTGTGTTTTCACGAAGATG+AGG - chr8_2:7974049-7974068 Msa1231700:three_prime_UTR 40.0%
GGATCTCATTGACGTTGTTT+CGG - chr8_2:7974070-7974089 Msa1231700:three_prime_UTR 40.0%
TCGGAATCTATAGCTAGGTT+TGG - chr8_2:7974089-7974108 Msa1231700:three_prime_UTR 40.0%
GGAAGTTGAATCCACAACTT+TGG + chr8_2:7974521-7974540 None:intergenic 40.0%
ACACACACTCATTTGGGAAA+TGG - chr8_2:7975083-7975102 Msa1231700:intron 40.0%
TACAGGATAGTGAGAATCCA+TGG - chr8_2:7975472-7975491 Msa1231700:intron 40.0%
CTAAAACACAAGCTCAACCA+TGG + chr8_2:7975492-7975511 None:intergenic 40.0%
! GAAGGTGGCTGAGAAAATTT+TGG - chr8_2:7975998-7976017 Msa1231700:intron 40.0%
ATCTCCAGCAGTGATTACTA+GGG + chr8_2:7976300-7976319 None:intergenic 40.0%
ATTGGCACACTAGCATTAAG+AGG + chr8_2:7976457-7976476 None:intergenic 40.0%
GGGCATTACAAGGATGTAAT+TGG - chr8_2:7976587-7976606 Msa1231700:CDS 40.0%
!! TGGCATTTTGAGGAGAATAG+AGG + chr8_2:7976661-7976680 None:intergenic 40.0%
TTGCTGCACATACTGAGATT+TGG - chr8_2:7977042-7977061 Msa1231700:CDS 40.0%
TGCTGCACATACTGAGATTT+GGG - chr8_2:7977043-7977062 Msa1231700:CDS 40.0%
GGGAACTATAAGCAAATGAC+TGG - chr8_2:7977063-7977082 Msa1231700:CDS 40.0%
TGAACAGAATCATGCTTGCT+CGG + chr8_2:7977325-7977344 None:intergenic 40.0%
ATTAACACCGCAAACCATGC+CGG - chr8_2:7973836-7973855 Msa1231700:three_prime_UTR 45.0%
TGTTACCGTTGATCTACGAC+CGG + chr8_2:7973907-7973926 None:intergenic 45.0%
CGAATTTCTCACCGAGTTTC+TGG - chr8_2:7973932-7973951 Msa1231700:three_prime_UTR 45.0%
GAAACGAAATCGATCTTCGG+CGG + chr8_2:7974029-7974048 None:intergenic 45.0%
GGTTGCCGGAGGAATATTTA+CGG - chr8_2:7974169-7974188 Msa1231700:exon 45.0%
!! AGGAATATTTACGGTGCCGT+CGG - chr8_2:7974178-7974197 Msa1231700:CDS 45.0%
TGATACTCTTCAAAACCCCG+AGG + chr8_2:7974239-7974258 None:intergenic 45.0%
!! TTGGGTTTTGACGGGAAACA+AGG - chr8_2:7974979-7974998 Msa1231700:CDS 45.0%
!! TGGGTTTTGACGGGAAACAA+GGG - chr8_2:7974980-7974999 Msa1231700:CDS 45.0%
GATAAGGAGTCGTTAGAGAG+CGG - chr8_2:7975380-7975399 Msa1231700:intron 45.0%
AGTCGTTAGAGAGCGGTAAA+AGG - chr8_2:7975387-7975406 Msa1231700:intron 45.0%
CAATGGCTAGCAATAGTCGT+TGG + chr8_2:7975446-7975465 None:intergenic 45.0%
ACGACTATTGCTAGCCATTG+AGG - chr8_2:7975446-7975465 Msa1231700:intron 45.0%
ACTATCCTGTAACGCCTCAA+TGG + chr8_2:7975463-7975482 None:intergenic 45.0%
GGAAACTCTACAATCACGTG+TGG - chr8_2:7975974-7975993 Msa1231700:intron 45.0%
TCTACAATCACGTGTGGAGA+AGG - chr8_2:7975980-7975999 Msa1231700:intron 45.0%
TTGTCTTTCTGCCCTGAACA+AGG - chr8_2:7976272-7976291 Msa1231700:intron 45.0%
! TTACTAGGGCACCTTGTTCA+GGG + chr8_2:7976286-7976305 None:intergenic 45.0%
! ATTACTAGGGCACCTTGTTC+AGG + chr8_2:7976287-7976306 None:intergenic 45.0%
GATCTCCAGCAGTGATTACT+AGG + chr8_2:7976301-7976320 None:intergenic 45.0%
!! ATTGAGTGGAGGGCATTACA+AGG - chr8_2:7976577-7976596 Msa1231700:intron 45.0%
! GCTTGTTGGCCAAGTGAAAT+AGG + chr8_2:7976710-7976729 None:intergenic 45.0%
! TTACAGCCAAGATGGCTTGT+TGG + chr8_2:7976724-7976743 None:intergenic 45.0%
TCATGCTTGCTCGGATGATT+TGG + chr8_2:7977316-7977335 None:intergenic 45.0%
!!! TAAAAAAGTATATATAATAT+TGG - chr8_2:7974960-7974979 Msa1231700:CDS 5.0%
!!! AAAAAAGTATATATAATATT+GGG - chr8_2:7974961-7974980 Msa1231700:CDS 5.0%
CGGAAATCAACGGTGGTTTC+CGG + chr8_2:7973858-7973877 None:intergenic 50.0%
CAATTCGCGGCGATATTCCA+TGG + chr8_2:7974133-7974152 None:intergenic 50.0%
TGGAATATCGCCGCGAATTG+TGG - chr8_2:7974133-7974152 Msa1231700:three_prime_UTR 50.0%
CGGCACCGTAAATATTCCTC+CGG + chr8_2:7974177-7974196 None:intergenic 50.0%
!! GGAATATTTACGGTGCCGTC+GGG - chr8_2:7974179-7974198 Msa1231700:CDS 50.0%
ACAGCATCTCTCATTCCCGA+CGG + chr8_2:7974197-7974216 None:intergenic 50.0%
TCAAAACCCCGAGGTTTCTC+CGG + chr8_2:7974230-7974249 None:intergenic 50.0%
TTGGAGGGAATTTGGCCTGT+TGG - chr8_2:7974344-7974363 Msa1231700:CDS 50.0%
!! CATTGACTTGAGCGTCAGAG+TGG + chr8_2:7975269-7975288 None:intergenic 50.0%
TAGATTCCGTGCAACTGGAG+GGG + chr8_2:7975634-7975653 None:intergenic 50.0%
CTAGATTCCGTGCAACTGGA+GGG + chr8_2:7975635-7975654 None:intergenic 50.0%
GAACCTAGATTCCGTGCAAC+TGG + chr8_2:7975639-7975658 None:intergenic 50.0%
ACAATCACGTGTGGAGAAGG+TGG - chr8_2:7975983-7976002 Msa1231700:intron 50.0%
CACTGCTGGAGATCAAATCC+AGG - chr8_2:7976307-7976326 Msa1231700:intron 50.0%
!! GCTTGGCTATAGGCATTGAG+TGG - chr8_2:7976563-7976582 Msa1231700:intron 50.0%
!! TGGCTATAGGCATTGAGTGG+AGG - chr8_2:7976566-7976585 Msa1231700:intron 50.0%
!! GGCTATAGGCATTGAGTGGA+GGG - chr8_2:7976567-7976586 Msa1231700:intron 50.0%
ACTTGGCCAACAAGCCATCT+TGG - chr8_2:7976715-7976734 Msa1231700:CDS 50.0%
GTGGTTTCCGGCATGGTTTG+CGG + chr8_2:7973846-7973865 None:intergenic 55.0%
ATCAACGGTGGTTTCCGGCA+TGG + chr8_2:7973853-7973872 None:intergenic 55.0%
AGCCTCCGGTCGTAGATCAA+CGG - chr8_2:7973899-7973918 Msa1231700:three_prime_UTR 55.0%
TACCGTTGATCTACGACCGG+AGG + chr8_2:7973904-7973923 None:intergenic 55.0%
ACTGCAGCAGCCACAATTCG+CGG + chr8_2:7974146-7974165 None:intergenic 55.0%
AATTGTGGCTGCTGCAGTGG+AGG - chr8_2:7974148-7974167 Msa1231700:three_prime_UTR 55.0%
AGATGCTGTGACGAGATCGC+CGG - chr8_2:7974208-7974227 Msa1231700:CDS 55.0%
TTGAGCGTCAGAGTGGCTAC+AGG + chr8_2:7975262-7975281 None:intergenic 55.0%
! GCTAGCCATTGAGGCGTTAC+AGG - chr8_2:7975455-7975474 Msa1231700:intron 55.0%
AGATTCCCCCTCCAGTTGCA+CGG - chr8_2:7975625-7975644 Msa1231700:intron 55.0%
AGATTCCGTGCAACTGGAGG+GGG + chr8_2:7975633-7975652 None:intergenic 55.0%
CCTAGATTCCGTGCAACTGG+AGG + chr8_2:7975636-7975655 None:intergenic 55.0%
CCTCCAGTTGCACGGAATCT+AGG - chr8_2:7975633-7975652 Msa1231700:intron 55.0%
GGTGCCCTAGTAATCACTGC+TGG - chr8_2:7976293-7976312 Msa1231700:intron 55.0%
CGGTGCACGGAAATCAACGG+TGG + chr8_2:7973865-7973884 None:intergenic 60.0%
CGGCGGTGCACGGAAATCAA+CGG + chr8_2:7973868-7973887 None:intergenic 60.0%
GATCTACGACCGGAGGCTAC+CGG + chr8_2:7973897-7973916 None:intergenic 60.0%
GCGAATTGTGGCTGCTGCAG+TGG - chr8_2:7974145-7974164 Msa1231700:three_prime_UTR 60.0%
CGAGATCGCCGGAGAAACCT+CGG - chr8_2:7974219-7974238 Msa1231700:CDS 60.0%
GAGATCGCCGGAGAAACCTC+GGG - chr8_2:7974220-7974239 Msa1231700:CDS 60.0%
AGATCGCCGGAGAAACCTCG+GGG - chr8_2:7974221-7974240 Msa1231700:CDS 60.0%
GCTGCTGCAGTGGAGGTTGC+CGG - chr8_2:7974155-7974174 Msa1231700:exon 65.0%
!! GAGGCTACCGGTGATGGCGG+CGG + chr8_2:7973885-7973904 None:intergenic 70.0%
! CCGGAGGCTACCGGTGATGG+CGG + chr8_2:7973888-7973907 None:intergenic 70.0%
CCGCCATCACCGGTAGCCTC+CGG - chr8_2:7973885-7973904 Msa1231700:three_prime_UTR 70.0%
CGACCGGAGGCTACCGGTGA+TGG + chr8_2:7973891-7973910 None:intergenic 70.0%
GCTGCAGTGGAGGTTGCCGG+AGG - chr8_2:7974158-7974177 Msa1231700:exon 70.0%
!! CCGGTGATGGCGGCGGTGCA+CGG + chr8_2:7973878-7973897 None:intergenic 75.0%
CCGTGCACCGCCGCCATCAC+CGG - chr8_2:7973875-7973894 Msa1231700:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 7973599 7977367 7973599 ID=Msa1231700;Name=Msa1231700
chr8_2 mRNA 7973599 7977367 7973599 ID=Msa1231700-mRNA-1;Parent=Msa1231700;Name=Msa1231700-mRNA-1;_AED=0.03;_eAED=0.03;_QI=223|1|1|1|1|1|3|574|380
chr8_2 exon 7973599 7974391 7973599 ID=Msa1231700-mRNA-1:exon:2999;Parent=Msa1231700-mRNA-1
chr8_2 exon 7974638 7975001 7974638 ID=Msa1231700-mRNA-1:exon:2998;Parent=Msa1231700-mRNA-1
chr8_2 exon 7976585 7977367 7976585 ID=Msa1231700-mRNA-1:exon:2997;Parent=Msa1231700-mRNA-1
chr8_2 five_prime_UTR 7977145 7977367 7977145 ID=Msa1231700-mRNA-1:five_prime_utr;Parent=Msa1231700-mRNA-1
chr8_2 CDS 7976585 7977144 7976585 ID=Msa1231700-mRNA-1:cds;Parent=Msa1231700-mRNA-1
chr8_2 CDS 7974638 7975001 7974638 ID=Msa1231700-mRNA-1:cds;Parent=Msa1231700-mRNA-1
chr8_2 CDS 7974173 7974391 7974173 ID=Msa1231700-mRNA-1:cds;Parent=Msa1231700-mRNA-1
chr8_2 three_prime_UTR 7973599 7974172 7973599 ID=Msa1231700-mRNA-1:three_prime_utr;Parent=Msa1231700-mRNA-1
Gene Sequence

>Msa1231700

ATGCGATCATTAATATTAACACCGCAAACCATGCCGGAAACCACCGTTGATTTCCGTGCACCGCCGCCATCACCGGTAGCCTCCGGTCGTAGATCAACGGTAACAAACAACGAATTTCTCACCGAGTTTCTGGAAACTTCGCTTCGAGTTCCAGATCTAGTATTACCGGACAAGATTTTCCCGAAACAGAGCAATGACGAAACGCCGCCGAAGATCGATTTCGTTTCACTGTGTTTTCACGAAGATGAGGATCTCATTGACGTTGTTTCGGAATCTATAGCTAGGTTTGGATGCTTTCAGCTTATTAACCATGGAATATCGCCGCGAATTGTGGCTGCTGCAGTGGAGGTTGCCGGAGGAATATTTACGGTGCCGTCGGGAATGAGAGATGCTGTGACGAGATCGCCGGAGAAACCTCGGGGTTTTGAAGAGTATCATGATGAAGTAGAAGAAGAAGAGGAGAGAAGAGAGTTGAATGAAGAATTTATTTGGTGTAAGAATGATGAAGAGTTGAAGTTGAAATTGGAGGGAATTTGGCCTGTTGGATATCCTAATTTTAGCAAGAAGATGGAAACTCTACAATCACGTGTGGAGAAGGTGGCTGAGAAAATTTTGGATATCATATTGAAAAATGTTAAAAAGAAATTAGCAAGTGATGGTGATATAATTGGTCATAGAAATGAAGTTGGGACACTTTGTTGCATTTACAAGCATCATCAAGATATTAATAGGAATGATCATTGGGTTGATTCTTTGAAATATGATGTGATTAGAATGCTGATTAGGGGAACTGATTACTCCCATTCTTTATGTTTGCATGTGTGTGATGGCTCTTCAGAGTTTCATGTTTACTCAAAGAAAAGTTGGTTGTCTTTCTGCCCTGAACAAGGTGCCCTAGTAATCACTGCTGGAGATCAAATCCAGGCATTGAGTGGAGGGCATTACAAGGATGTAATTGGAAAGGCAATATTTAAAGGCAAAAGAGAAGACAACATTTCAATGGCTTTCCTCTATTCTCCTCAAAATGCCAAGAAACTTCAAACAAATATGGAAAGACCTATTTCACTTGGCCAACAAGCCATCTTGGCTGTAATTTTGAACCTTATGTACCACGTTCTAATATTTATCTTTAAAAAGGTGTGA
Protein sequence

>Msa1231700

MRSLILTPQTMPETTVDFRAPPPSPVASGRRSTVTNNEFLTEFLETSLRVPDLVLPDKIFPKQSNDETPPKIDFVSLCFHEDEDLIDVVSESIARFGCFQLINHGISPRIVAAAVEVAGGIFTVPSGMRDAVTRSPEKPRGFEEYHDEVEEEEERRELNEEFIWCKNDEELKLKLEGIWPVGYPNFSKKMETLQSRVEKVAEKILDIILKNVKKKLASDGDIIGHRNEVGTLCCIYKHHQDINRNDHWVDSLKYDVIRMLIRGTDYSHSLCLHVCDGSSEFHVYSKKSWLSFCPEQGALVITAGDQIQALSGGHYKDVIGKAIFKGKREDNISMAFLYSPQNAKKLQTNMERPISLGQQAILAVILNLMYHVLIFIFKKV*