AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1235010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1235010 MtrunA17_Chr8g0337411 99.099 111 1 0 2 112 1 111 8.18e-73 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1235010 AT5G64050.1 64.602 113 34 3 3 112 1 110 3.36e-36 129

Find 29 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAATTGAGGACACTGATT+TGG 0.335541 8_2:+12807814 Msa1235010:CDS
ATAGTTGAAGAGGGCAGTTC+TGG 0.337351 8_2:-12807309 None:intergenic
TTACACTAGGTTAAAATTGA+TGG 0.347117 8_2:-12807005 None:intergenic
CGTTTCGCTCCTTCTCCTAC+TGG 0.357577 8_2:+12807266 Msa1235010:CDS
GAGTAAGATTGATGAAGAAA+TGG 0.387861 8_2:-12807158 None:intergenic
TCTTCAACTATTTGTTTGCT+AGG 0.402742 8_2:+12807321 Msa1235010:CDS
GATTGATGAAGAAATGGAAA+TGG 0.406079 8_2:-12807152 None:intergenic
GGAGCGAAACGAACACGAAC+GGG 0.454822 8_2:-12807254 None:intergenic
TACACTAGGTTAAAATTGAT+GGG 0.467739 8_2:-12807004 None:intergenic
CAGCCATCATCATTTCGCTT+AGG 0.502171 8_2:-12807082 None:intergenic
AGAAATGGAAATGGAAGAGA+TGG 0.529029 8_2:-12807143 None:intergenic
GACAGAGAATTTGCGTCGAA+AGG 0.530254 8_2:-12807204 None:intergenic
ACTGGTAACCTTCATGTTGG+TGG 0.531616 8_2:+12807284 Msa1235010:CDS
GAGGTCTACAAGAGAATCTG+AGG 0.555129 8_2:+12807838 Msa1235010:CDS
GTTCCTAAGCGAAATGATGA+TGG 0.567358 8_2:+12807079 Msa1235010:exon
CCTACTGGTAACCTTCATGT+TGG 0.570578 8_2:+12807281 Msa1235010:CDS
TAGCAAACAAATAGTTGAAG+AGG 0.571397 8_2:-12807319 None:intergenic
AGGAGCGAAACGAACACGAA+CGG 0.576837 8_2:-12807255 None:intergenic
GAAACGAACACGAACGGGAA+GGG 0.586268 8_2:-12807249 None:intergenic
CGTTGAGTAACGGAAGTCCA+TGG 0.591189 8_2:+12807105 Msa1235010:CDS
TTCTGGCACCACCAACATGA+AGG 0.592711 8_2:-12807292 None:intergenic
GGAGTAGCAAGCTTTGTCCA+TGG 0.607246 8_2:-12807122 None:intergenic
CGAAACGAACACGAACGGGA+AGG 0.609015 8_2:-12807250 None:intergenic
TGAAGGTTACCAGTAGGAGA+AGG 0.614462 8_2:-12807275 None:intergenic
AGCAAACAAATAGTTGAAGA+GGG 0.622093 8_2:-12807318 None:intergenic
TTGAGGACACTGATTTGGAG+AGG 0.627958 8_2:+12807819 Msa1235010:CDS
ATGATGGCTGCGTTGAGTAA+CGG 0.640360 8_2:+12807095 Msa1235010:CDS
CCAACATGAAGGTTACCAGT+AGG 0.669006 8_2:-12807281 None:intergenic
GTCTACAAGAGAATCTGAGG+AGG 0.680680 8_2:+12807841 Msa1235010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATTCAATTTTTATTTC+TGG + chr8_2:12807389-12807408 Msa1235010:intron 10.0%
!!! AAAAATTCAATTTTTAATTC+TGG + chr8_2:12807523-12807542 Msa1235010:intron 10.0%
!!! AAAATTCAATTTTTAATTCT+GGG + chr8_2:12807524-12807543 Msa1235010:intron 10.0%
!!! AAAAATCAATTTTTTTATTC+TGG + chr8_2:12807617-12807636 Msa1235010:intron 10.0%
!!! AAAATCAATTTTTTTATTCT+GGG + chr8_2:12807618-12807637 Msa1235010:intron 10.0%
!!! AAAAATTCAATTTTTATTTC+TGG + chr8_2:12807389-12807408 Msa1235010:intron 10.0%
!!! AAAAATTCAATTTTTAATTC+TGG + chr8_2:12807523-12807542 Msa1235010:intron 10.0%
!!! AAAATTCAATTTTTAATTCT+GGG + chr8_2:12807524-12807543 Msa1235010:intron 10.0%
!!! AAAAATCAATTTTTTTATTC+TGG + chr8_2:12807617-12807636 Msa1235010:intron 10.0%
!!! AAAATCAATTTTTTTATTCT+GGG + chr8_2:12807618-12807637 Msa1235010:intron 10.0%
!!! GGTTTTCTATTTTTAATATT+CGG + chr8_2:12807443-12807462 Msa1235010:intron 15.0%
!! AAAAATCAGAATTTGATTAT+GGG + chr8_2:12807722-12807741 Msa1235010:intron 15.0%
!!! GGTTTTCTATTTTTAATATT+CGG + chr8_2:12807443-12807462 Msa1235010:intron 15.0%
!! AAAAATCAGAATTTGATTAT+GGG + chr8_2:12807722-12807741 Msa1235010:intron 15.0%
!! AAAAAGCAAAAAGAAATTGA+GGG - chr8_2:12807362-12807381 None:intergenic 20.0%
!!! CAATTTTTATTTCTGGATTT+TGG + chr8_2:12807396-12807415 Msa1235010:intron 20.0%
!!! TCTATTTTTAATATTCGGTT+TGG + chr8_2:12807448-12807467 Msa1235010:intron 20.0%
!!! GTTATGATTTTTTATGTTCT+GGG + chr8_2:12807555-12807574 Msa1235010:intron 20.0%
!! GAAAAATCAGAATTTGATTA+TGG + chr8_2:12807721-12807740 Msa1235010:intron 20.0%
!!! GTTTTAATTTTGTATGTTCT+GGG + chr8_2:12807753-12807772 Msa1235010:intron 20.0%
!! AAAAAGCAAAAAGAAATTGA+GGG - chr8_2:12807362-12807381 None:intergenic 20.0%
!!! CAATTTTTATTTCTGGATTT+TGG + chr8_2:12807396-12807415 Msa1235010:intron 20.0%
!!! TCTATTTTTAATATTCGGTT+TGG + chr8_2:12807448-12807467 Msa1235010:intron 20.0%
!!! GTTATGATTTTTTATGTTCT+GGG + chr8_2:12807555-12807574 Msa1235010:intron 20.0%
!! GAAAAATCAGAATTTGATTA+TGG + chr8_2:12807721-12807740 Msa1235010:intron 20.0%
!!! GTTTTAATTTTGTATGTTCT+GGG + chr8_2:12807753-12807772 Msa1235010:intron 20.0%
! TACACTAGGTTAAAATTGAT+GGG - chr8_2:12807007-12807026 None:intergenic 25.0%
! TTACACTAGGTTAAAATTGA+TGG - chr8_2:12807008-12807027 None:intergenic 25.0%
! CAAAAAGCAAAAAGAAATTG+AGG - chr8_2:12807363-12807382 None:intergenic 25.0%
!!! TGTTTTGAGTTTTTATGTCA+TGG + chr8_2:12807421-12807440 Msa1235010:intron 25.0%
!!! GTTTTGAGTTTTTATGTCAT+GGG + chr8_2:12807422-12807441 Msa1235010:intron 25.0%
!!! TGTTTTGATTTTTTGTGTTC+TGG + chr8_2:12807472-12807491 Msa1235010:intron 25.0%
!!! GGTTATGATTTTTTATGTTC+TGG + chr8_2:12807554-12807573 Msa1235010:intron 25.0%
!!! TTTTTTATTCTGGGTTTGAA+TGG + chr8_2:12807627-12807646 Msa1235010:intron 25.0%
!!! GGTTTTAATTTTGTATGTTC+TGG + chr8_2:12807752-12807771 Msa1235010:intron 25.0%
!!! AATGTTTTTTTCAGGTCTAA+AGG + chr8_2:12807776-12807795 Msa1235010:intron 25.0%
!!! GAAATTTGTTTTGAGAATTG+AGG + chr8_2:12807802-12807821 Msa1235010:CDS 25.0%
! TACACTAGGTTAAAATTGAT+GGG - chr8_2:12807007-12807026 None:intergenic 25.0%
! TTACACTAGGTTAAAATTGA+TGG - chr8_2:12807008-12807027 None:intergenic 25.0%
! CAAAAAGCAAAAAGAAATTG+AGG - chr8_2:12807363-12807382 None:intergenic 25.0%
!!! TGTTTTGAGTTTTTATGTCA+TGG + chr8_2:12807421-12807440 Msa1235010:intron 25.0%
!!! GTTTTGAGTTTTTATGTCAT+GGG + chr8_2:12807422-12807441 Msa1235010:intron 25.0%
!!! TGTTTTGATTTTTTGTGTTC+TGG + chr8_2:12807472-12807491 Msa1235010:intron 25.0%
!!! GGTTATGATTTTTTATGTTC+TGG + chr8_2:12807554-12807573 Msa1235010:intron 25.0%
!!! TTTTTTATTCTGGGTTTGAA+TGG + chr8_2:12807627-12807646 Msa1235010:intron 25.0%
!!! GGTTTTAATTTTGTATGTTC+TGG + chr8_2:12807752-12807771 Msa1235010:intron 25.0%
!!! AATGTTTTTTTCAGGTCTAA+AGG + chr8_2:12807776-12807795 Msa1235010:intron 25.0%
!!! GAAATTTGTTTTGAGAATTG+AGG + chr8_2:12807802-12807821 Msa1235010:CDS 25.0%
!!! ATGCGTTTTTTCTTTACACT+AGG - chr8_2:12807021-12807040 None:intergenic 30.0%
GATTGATGAAGAAATGGAAA+TGG - chr8_2:12807155-12807174 None:intergenic 30.0%
GAGTAAGATTGATGAAGAAA+TGG - chr8_2:12807161-12807180 None:intergenic 30.0%
AGCAAACAAATAGTTGAAGA+GGG - chr8_2:12807321-12807340 None:intergenic 30.0%
TAGCAAACAAATAGTTGAAG+AGG - chr8_2:12807322-12807341 None:intergenic 30.0%
TCTTCAACTATTTGTTTGCT+AGG + chr8_2:12807321-12807340 Msa1235010:CDS 30.0%
!! TTTTTAATTCTGGGTATTGC+TGG + chr8_2:12807533-12807552 Msa1235010:intron 30.0%
!!! GATTTTTTATGTTCTGGGTT+TGG + chr8_2:12807560-12807579 Msa1235010:intron 30.0%
!!! TGTTTTGAGTTTTCATGTTC+TGG + chr8_2:12807664-12807683 Msa1235010:intron 30.0%
TCAGAATTTGATTATGGGTT+TGG + chr8_2:12807727-12807746 Msa1235010:intron 30.0%
!!! TTTTTTTCAGGTCTAAAGGT+GGG + chr8_2:12807780-12807799 Msa1235010:intron 30.0%
!!! ATGCGTTTTTTCTTTACACT+AGG - chr8_2:12807021-12807040 None:intergenic 30.0%
GATTGATGAAGAAATGGAAA+TGG - chr8_2:12807155-12807174 None:intergenic 30.0%
GAGTAAGATTGATGAAGAAA+TGG - chr8_2:12807161-12807180 None:intergenic 30.0%
AGCAAACAAATAGTTGAAGA+GGG - chr8_2:12807321-12807340 None:intergenic 30.0%
TAGCAAACAAATAGTTGAAG+AGG - chr8_2:12807322-12807341 None:intergenic 30.0%
TCTTCAACTATTTGTTTGCT+AGG + chr8_2:12807321-12807340 Msa1235010:CDS 30.0%
!! TTTTTAATTCTGGGTATTGC+TGG + chr8_2:12807533-12807552 Msa1235010:intron 30.0%
!!! GATTTTTTATGTTCTGGGTT+TGG + chr8_2:12807560-12807579 Msa1235010:intron 30.0%
!!! TGTTTTGAGTTTTCATGTTC+TGG + chr8_2:12807664-12807683 Msa1235010:intron 30.0%
TCAGAATTTGATTATGGGTT+TGG + chr8_2:12807727-12807746 Msa1235010:intron 30.0%
!!! TTTTTTTCAGGTCTAAAGGT+GGG + chr8_2:12807780-12807799 Msa1235010:intron 30.0%
AGAAATGGAAATGGAAGAGA+TGG - chr8_2:12807146-12807165 None:intergenic 35.0%
! ATTCTGGGTTTGAATGGTTT+TGG + chr8_2:12807633-12807652 Msa1235010:intron 35.0%
! TTCTGGGTTTGAATGGTTTT+GGG + chr8_2:12807634-12807653 Msa1235010:intron 35.0%
!!! GAGTTTTCATGTTCTGGTTT+TGG + chr8_2:12807670-12807689 Msa1235010:intron 35.0%
AATTTGATTATGGGTTTGGC+TGG + chr8_2:12807731-12807750 Msa1235010:intron 35.0%
!!! GTTCTGGGAATGTTTTTTTC+AGG + chr8_2:12807768-12807787 Msa1235010:intron 35.0%
!!! GTTTTTTTCAGGTCTAAAGG+TGG + chr8_2:12807779-12807798 Msa1235010:intron 35.0%
AGAAATGGAAATGGAAGAGA+TGG - chr8_2:12807146-12807165 None:intergenic 35.0%
! ATTCTGGGTTTGAATGGTTT+TGG + chr8_2:12807633-12807652 Msa1235010:intron 35.0%
! TTCTGGGTTTGAATGGTTTT+GGG + chr8_2:12807634-12807653 Msa1235010:intron 35.0%
!!! GAGTTTTCATGTTCTGGTTT+TGG + chr8_2:12807670-12807689 Msa1235010:intron 35.0%
AATTTGATTATGGGTTTGGC+TGG + chr8_2:12807731-12807750 Msa1235010:intron 35.0%
!!! GTTCTGGGAATGTTTTTTTC+AGG + chr8_2:12807768-12807787 Msa1235010:intron 35.0%
!!! GTTTTTTTCAGGTCTAAAGG+TGG + chr8_2:12807779-12807798 Msa1235010:intron 35.0%
GTTCCTAAGCGAAATGATGA+TGG + chr8_2:12807079-12807098 Msa1235010:exon 40.0%
!!! GGTTTGAATGGTTTTGGGTT+TGG + chr8_2:12807639-12807658 Msa1235010:intron 40.0%
! GAGAATTGAGGACACTGATT+TGG + chr8_2:12807814-12807833 Msa1235010:CDS 40.0%
GTTCCTAAGCGAAATGATGA+TGG + chr8_2:12807079-12807098 Msa1235010:exon 40.0%
!!! GGTTTGAATGGTTTTGGGTT+TGG + chr8_2:12807639-12807658 Msa1235010:intron 40.0%
! GAGAATTGAGGACACTGATT+TGG + chr8_2:12807814-12807833 Msa1235010:CDS 40.0%
CAGCCATCATCATTTCGCTT+AGG - chr8_2:12807085-12807104 None:intergenic 45.0%
ATGATGGCTGCGTTGAGTAA+CGG + chr8_2:12807095-12807114 Msa1235010:CDS 45.0%
GACAGAGAATTTGCGTCGAA+AGG - chr8_2:12807207-12807226 None:intergenic 45.0%
TGAAGGTTACCAGTAGGAGA+AGG - chr8_2:12807278-12807297 None:intergenic 45.0%
CCAACATGAAGGTTACCAGT+AGG - chr8_2:12807284-12807303 None:intergenic 45.0%
CCTACTGGTAACCTTCATGT+TGG + chr8_2:12807281-12807300 Msa1235010:CDS 45.0%
ACTGGTAACCTTCATGTTGG+TGG + chr8_2:12807284-12807303 Msa1235010:CDS 45.0%
ATAGTTGAAGAGGGCAGTTC+TGG - chr8_2:12807312-12807331 None:intergenic 45.0%
!! TTGAGGACACTGATTTGGAG+AGG + chr8_2:12807819-12807838 Msa1235010:CDS 45.0%
GAGGTCTACAAGAGAATCTG+AGG + chr8_2:12807838-12807857 Msa1235010:CDS 45.0%
GTCTACAAGAGAATCTGAGG+AGG + chr8_2:12807841-12807860 Msa1235010:CDS 45.0%
CAGCCATCATCATTTCGCTT+AGG - chr8_2:12807085-12807104 None:intergenic 45.0%
ATGATGGCTGCGTTGAGTAA+CGG + chr8_2:12807095-12807114 Msa1235010:CDS 45.0%
GACAGAGAATTTGCGTCGAA+AGG - chr8_2:12807207-12807226 None:intergenic 45.0%
TGAAGGTTACCAGTAGGAGA+AGG - chr8_2:12807278-12807297 None:intergenic 45.0%
CCAACATGAAGGTTACCAGT+AGG - chr8_2:12807284-12807303 None:intergenic 45.0%
CCTACTGGTAACCTTCATGT+TGG + chr8_2:12807281-12807300 Msa1235010:CDS 45.0%
ACTGGTAACCTTCATGTTGG+TGG + chr8_2:12807284-12807303 Msa1235010:CDS 45.0%
ATAGTTGAAGAGGGCAGTTC+TGG - chr8_2:12807312-12807331 None:intergenic 45.0%
!! TTGAGGACACTGATTTGGAG+AGG + chr8_2:12807819-12807838 Msa1235010:CDS 45.0%
GAGGTCTACAAGAGAATCTG+AGG + chr8_2:12807838-12807857 Msa1235010:CDS 45.0%
GTCTACAAGAGAATCTGAGG+AGG + chr8_2:12807841-12807860 Msa1235010:CDS 45.0%
! CGTTGAGTAACGGAAGTCCA+TGG + chr8_2:12807105-12807124 Msa1235010:CDS 50.0%
! GGAGTAGCAAGCTTTGTCCA+TGG - chr8_2:12807125-12807144 None:intergenic 50.0%
GAAACGAACACGAACGGGAA+GGG - chr8_2:12807252-12807271 None:intergenic 50.0%
AGGAGCGAAACGAACACGAA+CGG - chr8_2:12807258-12807277 None:intergenic 50.0%
TTCTGGCACCACCAACATGA+AGG - chr8_2:12807295-12807314 None:intergenic 50.0%
! CGTTGAGTAACGGAAGTCCA+TGG + chr8_2:12807105-12807124 Msa1235010:CDS 50.0%
! GGAGTAGCAAGCTTTGTCCA+TGG - chr8_2:12807125-12807144 None:intergenic 50.0%
GAAACGAACACGAACGGGAA+GGG - chr8_2:12807252-12807271 None:intergenic 50.0%
AGGAGCGAAACGAACACGAA+CGG - chr8_2:12807258-12807277 None:intergenic 50.0%
TTCTGGCACCACCAACATGA+AGG - chr8_2:12807295-12807314 None:intergenic 50.0%
CGAAACGAACACGAACGGGA+AGG - chr8_2:12807253-12807272 None:intergenic 55.0%
GGAGCGAAACGAACACGAAC+GGG - chr8_2:12807257-12807276 None:intergenic 55.0%
CGTTTCGCTCCTTCTCCTAC+TGG + chr8_2:12807266-12807285 Msa1235010:CDS 55.0%
CGAAACGAACACGAACGGGA+AGG - chr8_2:12807253-12807272 None:intergenic 55.0%
GGAGCGAAACGAACACGAAC+GGG - chr8_2:12807257-12807276 None:intergenic 55.0%
CGTTTCGCTCCTTCTCCTAC+TGG + chr8_2:12807266-12807285 Msa1235010:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 12806994 12807874 12806994 ID=Msa1235010;Name=Msa1235010
chr8_2 mRNA 12806994 12807874 12806994 ID=Msa1235010-mRNA-1;Parent=Msa1235010;Name=Msa1235010-mRNA-1;_AED=0.00;_eAED=0.00;_QI=98|1|0.5|1|1|1|2|0|112
chr8_2 exon 12806994 12807342 12806994 ID=Msa1235010-mRNA-1:exon:4981;Parent=Msa1235010-mRNA-1
chr8_2 exon 12807790 12807874 12807790 ID=Msa1235010-mRNA-1:exon:4982;Parent=Msa1235010-mRNA-1
chr8_2 five_prime_UTR 12806994 12807091 12806994 ID=Msa1235010-mRNA-1:five_prime_utr;Parent=Msa1235010-mRNA-1
chr8_2 CDS 12807092 12807342 12807092 ID=Msa1235010-mRNA-1:cds;Parent=Msa1235010-mRNA-1
chr8_2 CDS 12807790 12807874 12807790 ID=Msa1235010-mRNA-1:cds;Parent=Msa1235010-mRNA-1
Gene Sequence

>Msa1235010

ATGATGATGGCTGCGTTGAGTAACGGAAGTCCATGGACAAAGCTTGCTACTCCATCTCTTCCATTTCCATTTCTTCATCAATCTTACTCTTCTTACACTTCACGAATCTTCTCCTTTCGACGCAAATTCTCTGTCAATGCTCTTTCACAACAACAATCCCTTCCCGTTCGTGTTCGTTTCGCTCCTTCTCCTACTGGTAACCTTCATGTTGGTGGTGCCAGAACTGCCCTCTTCAACTATTTGTTTGCTAGGTCTAAAGGTGGGAAATTTGTTTTGAGAATTGAGGACACTGATTTGGAGAGGTCTACAAGAGAATCTGAGGAGGCAGTGCTTAGA

Protein sequence

>Msa1235010

MMMAALSNGSPWTKLATPSLPFPFLHQSYSSYTSRIFSFRRKFSVNALSQQQSLPVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTRESEEAVLR