AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1264260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCAGGAGATTCAGATATT+AGG 0.318752 8_2:+67700306 Msa1264260:CDS
ACGTGGAGATATCTTATTCC+AGG 0.408219 8_2:+67701802 Msa1264260:CDS
TTATATGAAATGCAGTACTA+AGG 0.431580 8_2:+67701747 Msa1264260:intron
ATAATTATACATTTGGATCC+TGG 0.444469 8_2:-67701820 None:intergenic
TACCTAATATCTGAATCTCC+TGG 0.458804 8_2:-67700308 None:intergenic
GAGAAATTGGATATAGCAAA+GGG 0.467145 8_2:+67700227 None:intergenic
CATAGGTGTATCGCAGTTTG+AGG 0.468479 8_2:-67701444 None:intergenic
ACTAAGGGCTATATATATGC+AGG 0.530303 8_2:+67701763 Msa1264260:CDS
AGAGAAATTGGATATAGCAA+AGG 0.534255 8_2:+67700226 None:intergenic
ACCTAATATCTGAATCTCCT+GGG 0.537940 8_2:-67700307 None:intergenic
TTGAGGGAAGCATTCATCCC+AGG 0.562194 8_2:+67700290 Msa1264260:CDS
TATATGAAATGCAGTACTAA+GGG 0.566046 8_2:+67701748 Msa1264260:intron
CTATATATATGCAGGTCACA+AGG 0.606317 8_2:+67701771 Msa1264260:CDS
GTCACAAGGAATGCTATACG+TGG 0.695996 8_2:+67701785 Msa1264260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAACTTAATGTAAAATA+AGG - chr8_2:67700566-67700585 None:intergenic 10.0%
!!! AGAAAAGTATTAAATAATTT+AGG + chr8_2:67700838-67700857 Msa1264260:intron 10.0%
!!! TTAATTATGTCTTTTTATTA+TGG + chr8_2:67701241-67701260 Msa1264260:intron 10.0%
!!! TAATTATGTCTTTTTATTAT+GGG + chr8_2:67701242-67701261 Msa1264260:intron 10.0%
!! AATTAACTTAATGTAAAATA+AGG - chr8_2:67700566-67700585 None:intergenic 10.0%
!!! AGAAAAGTATTAAATAATTT+AGG + chr8_2:67700838-67700857 Msa1264260:intron 10.0%
!!! TTAATTATGTCTTTTTATTA+TGG + chr8_2:67701241-67701260 Msa1264260:intron 10.0%
!!! TAATTATGTCTTTTTATTAT+GGG + chr8_2:67701242-67701261 Msa1264260:intron 10.0%
!! AATATTATGTTGAAGAAAAT+AGG - chr8_2:67700745-67700764 None:intergenic 15.0%
!!! ATTTTCTTCAACATAATATT+AGG + chr8_2:67700745-67700764 Msa1264260:intron 15.0%
!!! TTTCTTATCTAATTTTGTAT+TGG + chr8_2:67701318-67701337 Msa1264260:intron 15.0%
!! AATATTATGTTGAAGAAAAT+AGG - chr8_2:67700745-67700764 None:intergenic 15.0%
!!! ATTTTCTTCAACATAATATT+AGG + chr8_2:67700745-67700764 Msa1264260:intron 15.0%
!!! TTTCTTATCTAATTTTGTAT+TGG + chr8_2:67701318-67701337 Msa1264260:intron 15.0%
!!! TCTAAGTTGAAAAGAATAAA+AGG + chr8_2:67700357-67700376 Msa1264260:intron 20.0%
!!! ATGTTGTTTGTAATTTTCTA+GGG + chr8_2:67700804-67700823 Msa1264260:intron 20.0%
!! ATTTATCCATCTTAATGTAA+CGG - chr8_2:67701075-67701094 None:intergenic 20.0%
!! TTACATTAAGATGGATAAAT+AGG + chr8_2:67701075-67701094 Msa1264260:intron 20.0%
!! GATGATTTATGAATAGTTAA+AGG - chr8_2:67701275-67701294 None:intergenic 20.0%
!! AATCATCTCTTATTCTTAAT+TGG + chr8_2:67701288-67701307 Msa1264260:intron 20.0%
!! AATTTCATGATAGAAAAGAT+AGG + chr8_2:67701512-67701531 Msa1264260:intron 20.0%
!!! AATTTTTTTTAAAGCATGTG+AGG - chr8_2:67701593-67701612 None:intergenic 20.0%
!!! TCACATGCTTTAAAAAAAAT+TGG + chr8_2:67701592-67701611 Msa1264260:intron 20.0%
!!! TCTAAGTTGAAAAGAATAAA+AGG + chr8_2:67700357-67700376 Msa1264260:intron 20.0%
!!! ATGTTGTTTGTAATTTTCTA+GGG + chr8_2:67700804-67700823 Msa1264260:intron 20.0%
!! ATTTATCCATCTTAATGTAA+CGG - chr8_2:67701075-67701094 None:intergenic 20.0%
!! TTACATTAAGATGGATAAAT+AGG + chr8_2:67701075-67701094 Msa1264260:intron 20.0%
!! GATGATTTATGAATAGTTAA+AGG - chr8_2:67701275-67701294 None:intergenic 20.0%
!! AATCATCTCTTATTCTTAAT+TGG + chr8_2:67701288-67701307 Msa1264260:intron 20.0%
!! AATTTCATGATAGAAAAGAT+AGG + chr8_2:67701512-67701531 Msa1264260:intron 20.0%
!!! AATTTTTTTTAAAGCATGTG+AGG - chr8_2:67701593-67701612 None:intergenic 20.0%
!!! TCACATGCTTTAAAAAAAAT+TGG + chr8_2:67701592-67701611 Msa1264260:intron 20.0%
!! ACTATGATGTTTTACATTTG+AGG + chr8_2:67700273-67700292 Msa1264260:CDS 25.0%
!! CTATGATGTTTTACATTTGA+GGG + chr8_2:67700274-67700293 Msa1264260:CDS 25.0%
! TAGAGATATATAAAACCCAT+TGG - chr8_2:67700341-67700360 None:intergenic 25.0%
! TTATTTCAACAAGTTTAGCA+TGG - chr8_2:67700385-67700404 None:intergenic 25.0%
!!! AGACAAGGAGTTTTTTAAAA+AGG - chr8_2:67700409-67700428 None:intergenic 25.0%
!!! TTTTTTCTGTAAATCTCAAC+TGG + chr8_2:67700504-67700523 Msa1264260:intron 25.0%
! ACAACATCATATCAATATAG+TGG - chr8_2:67700791-67700810 None:intergenic 25.0%
!!! GATGTTGTTTGTAATTTTCT+AGG + chr8_2:67700803-67700822 Msa1264260:intron 25.0%
!! ACTAATTTGTAACAATCACA+TGG - chr8_2:67700875-67700894 None:intergenic 25.0%
! ACAATATGTCAATATCATAG+AGG + chr8_2:67700896-67700915 Msa1264260:intron 25.0%
! TGAATGTAATGCAAATTGAT+AGG + chr8_2:67701182-67701201 Msa1264260:intron 25.0%
!! TTTTAATACTAAGCTTGATC+TGG + chr8_2:67701346-67701365 Msa1264260:intron 25.0%
!! CTTGTATTAGTATTTTGTTG+TGG + chr8_2:67701373-67701392 Msa1264260:intron 25.0%
!! ATTTTACTAGCCATATTATC+AGG + chr8_2:67701406-67701425 Msa1264260:intron 25.0%
! TTATATGAAATGCAGTACTA+AGG + chr8_2:67701747-67701766 Msa1264260:intron 25.0%
! TATATGAAATGCAGTACTAA+GGG + chr8_2:67701748-67701767 Msa1264260:intron 25.0%
!! ACTATGATGTTTTACATTTG+AGG + chr8_2:67700273-67700292 Msa1264260:CDS 25.0%
!! CTATGATGTTTTACATTTGA+GGG + chr8_2:67700274-67700293 Msa1264260:CDS 25.0%
! TAGAGATATATAAAACCCAT+TGG - chr8_2:67700341-67700360 None:intergenic 25.0%
! TTATTTCAACAAGTTTAGCA+TGG - chr8_2:67700385-67700404 None:intergenic 25.0%
!!! AGACAAGGAGTTTTTTAAAA+AGG - chr8_2:67700409-67700428 None:intergenic 25.0%
!!! TTTTTTCTGTAAATCTCAAC+TGG + chr8_2:67700504-67700523 Msa1264260:intron 25.0%
! ACAACATCATATCAATATAG+TGG - chr8_2:67700791-67700810 None:intergenic 25.0%
!!! GATGTTGTTTGTAATTTTCT+AGG + chr8_2:67700803-67700822 Msa1264260:intron 25.0%
!! ACTAATTTGTAACAATCACA+TGG - chr8_2:67700875-67700894 None:intergenic 25.0%
! ACAATATGTCAATATCATAG+AGG + chr8_2:67700896-67700915 Msa1264260:intron 25.0%
! TGAATGTAATGCAAATTGAT+AGG + chr8_2:67701182-67701201 Msa1264260:intron 25.0%
!! TTTTAATACTAAGCTTGATC+TGG + chr8_2:67701346-67701365 Msa1264260:intron 25.0%
!! CTTGTATTAGTATTTTGTTG+TGG + chr8_2:67701373-67701392 Msa1264260:intron 25.0%
!! ATTTTACTAGCCATATTATC+AGG + chr8_2:67701406-67701425 Msa1264260:intron 25.0%
! TTATATGAAATGCAGTACTA+AGG + chr8_2:67701747-67701766 Msa1264260:intron 25.0%
! TATATGAAATGCAGTACTAA+GGG + chr8_2:67701748-67701767 Msa1264260:intron 25.0%
TATTAGGTAAAGCTTACCAA+TGG + chr8_2:67700322-67700341 Msa1264260:intron 30.0%
ATTAGGTAAAGCTTACCAAT+GGG + chr8_2:67700323-67700342 Msa1264260:intron 30.0%
! GTTTTATATCACTCTGATAC+AGG - chr8_2:67700456-67700475 None:intergenic 30.0%
TGTCAATATCATAGAGGAAA+AGG + chr8_2:67700902-67700921 Msa1264260:intron 30.0%
CAATTTGCATTACATTCACT+AGG - chr8_2:67701180-67701199 None:intergenic 30.0%
TATCTCTTAGCCTGATAATA+TGG - chr8_2:67701419-67701438 None:intergenic 30.0%
AATTGGTGCATTAAATTACC+AGG + chr8_2:67701609-67701628 Msa1264260:intron 30.0%
!!! GTTATTTTTGCTGACAACAA+AGG - chr8_2:67701663-67701682 None:intergenic 30.0%
TATTAGGTAAAGCTTACCAA+TGG + chr8_2:67700322-67700341 Msa1264260:intron 30.0%
ATTAGGTAAAGCTTACCAAT+GGG + chr8_2:67700323-67700342 Msa1264260:intron 30.0%
! GTTTTATATCACTCTGATAC+AGG - chr8_2:67700456-67700475 None:intergenic 30.0%
TGTCAATATCATAGAGGAAA+AGG + chr8_2:67700902-67700921 Msa1264260:intron 30.0%
CAATTTGCATTACATTCACT+AGG - chr8_2:67701180-67701199 None:intergenic 30.0%
TATCTCTTAGCCTGATAATA+TGG - chr8_2:67701419-67701438 None:intergenic 30.0%
AATTGGTGCATTAAATTACC+AGG + chr8_2:67701609-67701628 Msa1264260:intron 30.0%
!!! GTTATTTTTGCTGACAACAA+AGG - chr8_2:67701663-67701682 None:intergenic 30.0%
ACCTAATATCTGAATCTCCT+GGG - chr8_2:67700310-67700329 None:intergenic 35.0%
TACCTAATATCTGAATCTCC+TGG - chr8_2:67700311-67700330 None:intergenic 35.0%
! AAACTAAACCAGTTTGCTCT+AGG + chr8_2:67700472-67700491 Msa1264260:intron 35.0%
AATTCTATCCTAGAGCAAAC+TGG - chr8_2:67700483-67700502 None:intergenic 35.0%
!!! TGCTCAAGTTTTGTTGCATA+AGG - chr8_2:67700598-67700617 None:intergenic 35.0%
CACTATTCTGACTTTCCAAT+GGG - chr8_2:67700627-67700646 None:intergenic 35.0%
ATTGGAAAGTCAGAATAGTG+CGG + chr8_2:67700627-67700646 Msa1264260:intron 35.0%
! TAGAGACTGATTTCTATCCT+TGG + chr8_2:67700992-67701011 Msa1264260:intron 35.0%
ACAATGCCGTTACATTAAGA+TGG + chr8_2:67701066-67701085 Msa1264260:intron 35.0%
!!! ACGCTAGCAACACAATTTTT+TGG - chr8_2:67701156-67701175 None:intergenic 35.0%
ATGATGTACTGCACATACAT+AGG - chr8_2:67701464-67701483 None:intergenic 35.0%
!! GTGATCCTAGAAATTGGTTT+TGG + chr8_2:67701634-67701653 Msa1264260:intron 35.0%
GGTTACCAAAACCAATTTCT+AGG - chr8_2:67701642-67701661 None:intergenic 35.0%
ACTAAGGGCTATATATATGC+AGG + chr8_2:67701763-67701782 Msa1264260:CDS 35.0%
CTATATATATGCAGGTCACA+AGG + chr8_2:67701771-67701790 Msa1264260:CDS 35.0%
ACCTAATATCTGAATCTCCT+GGG - chr8_2:67700310-67700329 None:intergenic 35.0%
TACCTAATATCTGAATCTCC+TGG - chr8_2:67700311-67700330 None:intergenic 35.0%
! AAACTAAACCAGTTTGCTCT+AGG + chr8_2:67700472-67700491 Msa1264260:intron 35.0%
AATTCTATCCTAGAGCAAAC+TGG - chr8_2:67700483-67700502 None:intergenic 35.0%
!!! TGCTCAAGTTTTGTTGCATA+AGG - chr8_2:67700598-67700617 None:intergenic 35.0%
CACTATTCTGACTTTCCAAT+GGG - chr8_2:67700627-67700646 None:intergenic 35.0%
ATTGGAAAGTCAGAATAGTG+CGG + chr8_2:67700627-67700646 Msa1264260:intron 35.0%
! TAGAGACTGATTTCTATCCT+TGG + chr8_2:67700992-67701011 Msa1264260:intron 35.0%
ACAATGCCGTTACATTAAGA+TGG + chr8_2:67701066-67701085 Msa1264260:intron 35.0%
!!! ACGCTAGCAACACAATTTTT+TGG - chr8_2:67701156-67701175 None:intergenic 35.0%
ATGATGTACTGCACATACAT+AGG - chr8_2:67701464-67701483 None:intergenic 35.0%
!! GTGATCCTAGAAATTGGTTT+TGG + chr8_2:67701634-67701653 Msa1264260:intron 35.0%
GGTTACCAAAACCAATTTCT+AGG - chr8_2:67701642-67701661 None:intergenic 35.0%
ACTAAGGGCTATATATATGC+AGG + chr8_2:67701763-67701782 Msa1264260:CDS 35.0%
CTATATATATGCAGGTCACA+AGG + chr8_2:67701771-67701790 Msa1264260:CDS 35.0%
TCCCAGGAGATTCAGATATT+AGG + chr8_2:67700306-67700325 Msa1264260:CDS 40.0%
ACTTGAGCAATTCTTCCCAT+TGG + chr8_2:67700609-67700628 Msa1264260:intron 40.0%
GCACTATTCTGACTTTCCAA+TGG - chr8_2:67700628-67700647 None:intergenic 40.0%
TCTCTATCCATTGCTAGACA+CGG + chr8_2:67700932-67700951 Msa1264260:intron 40.0%
TACCTCCCTTGAGTAATGAA+GGG - chr8_2:67700974-67700993 None:intergenic 40.0%
GATTGAGAAATGGGAAACCA+AGG - chr8_2:67701012-67701031 None:intergenic 40.0%
GCACAGAAGGATTGAGAAAT+GGG - chr8_2:67701021-67701040 None:intergenic 40.0%
AGCACAGAAGGATTGAGAAA+TGG - chr8_2:67701022-67701041 None:intergenic 40.0%
CAATCCTTCTGTGCTTATGA+TGG + chr8_2:67701027-67701046 Msa1264260:intron 40.0%
GTGCAGTACATCATCACATA+AGG + chr8_2:67701470-67701489 Msa1264260:intron 40.0%
TAACAGTAGATATGCCTGTC+AGG + chr8_2:67701679-67701698 Msa1264260:intron 40.0%
! TCAGGCATTGTAACATGACA+TGG + chr8_2:67701697-67701716 Msa1264260:intron 40.0%
ACGTGGAGATATCTTATTCC+AGG + chr8_2:67701802-67701821 Msa1264260:CDS 40.0%
TCCCAGGAGATTCAGATATT+AGG + chr8_2:67700306-67700325 Msa1264260:CDS 40.0%
ACTTGAGCAATTCTTCCCAT+TGG + chr8_2:67700609-67700628 Msa1264260:intron 40.0%
GCACTATTCTGACTTTCCAA+TGG - chr8_2:67700628-67700647 None:intergenic 40.0%
TCTCTATCCATTGCTAGACA+CGG + chr8_2:67700932-67700951 Msa1264260:intron 40.0%
TACCTCCCTTGAGTAATGAA+GGG - chr8_2:67700974-67700993 None:intergenic 40.0%
GATTGAGAAATGGGAAACCA+AGG - chr8_2:67701012-67701031 None:intergenic 40.0%
GCACAGAAGGATTGAGAAAT+GGG - chr8_2:67701021-67701040 None:intergenic 40.0%
AGCACAGAAGGATTGAGAAA+TGG - chr8_2:67701022-67701041 None:intergenic 40.0%
CAATCCTTCTGTGCTTATGA+TGG + chr8_2:67701027-67701046 Msa1264260:intron 40.0%
GTGCAGTACATCATCACATA+AGG + chr8_2:67701470-67701489 Msa1264260:intron 40.0%
TAACAGTAGATATGCCTGTC+AGG + chr8_2:67701679-67701698 Msa1264260:intron 40.0%
! TCAGGCATTGTAACATGACA+TGG + chr8_2:67701697-67701716 Msa1264260:intron 40.0%
ACGTGGAGATATCTTATTCC+AGG + chr8_2:67701802-67701821 Msa1264260:CDS 40.0%
AGTGACAACTCAGACAGACA+AGG - chr8_2:67700424-67700443 None:intergenic 45.0%
TAGACACGGAGAGTAGTCTT+TGG + chr8_2:67700946-67700965 Msa1264260:intron 45.0%
CTACCTCCCTTGAGTAATGA+AGG - chr8_2:67700975-67700994 None:intergenic 45.0%
CTCACCATCATAAGCACAGA+AGG - chr8_2:67701034-67701053 None:intergenic 45.0%
CATAGGTGTATCGCAGTTTG+AGG - chr8_2:67701447-67701466 None:intergenic 45.0%
!! CAATTTCTAGGATCACAGCC+TGG - chr8_2:67701630-67701649 None:intergenic 45.0%
CAGGCTGTGATCCTAGAAAT+TGG + chr8_2:67701628-67701647 Msa1264260:intron 45.0%
GTCATGTTACAATGCCTGAC+AGG - chr8_2:67701696-67701715 None:intergenic 45.0%
GTCACAAGGAATGCTATACG+TGG + chr8_2:67701785-67701804 Msa1264260:CDS 45.0%
AGTGACAACTCAGACAGACA+AGG - chr8_2:67700424-67700443 None:intergenic 45.0%
TAGACACGGAGAGTAGTCTT+TGG + chr8_2:67700946-67700965 Msa1264260:intron 45.0%
CTACCTCCCTTGAGTAATGA+AGG - chr8_2:67700975-67700994 None:intergenic 45.0%
CTCACCATCATAAGCACAGA+AGG - chr8_2:67701034-67701053 None:intergenic 45.0%
CATAGGTGTATCGCAGTTTG+AGG - chr8_2:67701447-67701466 None:intergenic 45.0%
!! CAATTTCTAGGATCACAGCC+TGG - chr8_2:67701630-67701649 None:intergenic 45.0%
CAGGCTGTGATCCTAGAAAT+TGG + chr8_2:67701628-67701647 Msa1264260:intron 45.0%
GTCATGTTACAATGCCTGAC+AGG - chr8_2:67701696-67701715 None:intergenic 45.0%
GTCACAAGGAATGCTATACG+TGG + chr8_2:67701785-67701804 Msa1264260:CDS 45.0%
!! TTGAGGGAAGCATTCATCCC+AGG + chr8_2:67700290-67700309 Msa1264260:CDS 50.0%
GGAAAGTCAGAATAGTGCGG+TGG + chr8_2:67700630-67700649 Msa1264260:intron 50.0%
CTACTCTCCGTGTCTAGCAA+TGG - chr8_2:67700942-67700961 None:intergenic 50.0%
TTGGCGCCCTTCATTACTCA+AGG + chr8_2:67700965-67700984 Msa1264260:intron 50.0%
TGGCGCCCTTCATTACTCAA+GGG + chr8_2:67700966-67700985 Msa1264260:intron 50.0%
!! TTGAGGGAAGCATTCATCCC+AGG + chr8_2:67700290-67700309 Msa1264260:CDS 50.0%
GGAAAGTCAGAATAGTGCGG+TGG + chr8_2:67700630-67700649 Msa1264260:intron 50.0%
CTACTCTCCGTGTCTAGCAA+TGG - chr8_2:67700942-67700961 None:intergenic 50.0%
TTGGCGCCCTTCATTACTCA+AGG + chr8_2:67700965-67700984 Msa1264260:intron 50.0%
TGGCGCCCTTCATTACTCAA+GGG + chr8_2:67700966-67700985 Msa1264260:intron 50.0%
CGCCCTTCATTACTCAAGGG+AGG + chr8_2:67700969-67700988 Msa1264260:intron 55.0%
CGCCCTTCATTACTCAAGGG+AGG + chr8_2:67700969-67700988 Msa1264260:intron 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 67700233 67701838 67700233 ID=Msa1264260;Name=Msa1264260
chr8_2 mRNA 67700233 67701838 67700233 ID=Msa1264260-mRNA-1;Parent=Msa1264260;Name=Msa1264260-mRNA-1;_AED=0.57;_eAED=0.76;_QI=0|0|0|0.66|0|0|3|0|69
chr8_2 exon 67700233 67700327 67700233 ID=Msa1264260-mRNA-1:exon:22340;Parent=Msa1264260-mRNA-1
chr8_2 exon 67701428 67701465 67701428 ID=Msa1264260-mRNA-1:exon:22341;Parent=Msa1264260-mRNA-1
chr8_2 exon 67701762 67701838 67701762 ID=Msa1264260-mRNA-1:exon:22342;Parent=Msa1264260-mRNA-1
chr8_2 CDS 67700233 67700327 67700233 ID=Msa1264260-mRNA-1:cds;Parent=Msa1264260-mRNA-1
chr8_2 CDS 67701428 67701465 67701428 ID=Msa1264260-mRNA-1:cds;Parent=Msa1264260-mRNA-1
chr8_2 CDS 67701762 67701838 67701762 ID=Msa1264260-mRNA-1:cds;Parent=Msa1264260-mRNA-1
Gene Sequence

>Msa1264260

TTGGATATAGCAAAGGGTTTATCTCATTACTACATAGCTGACTATGATGTTTTACATTTGAGGGAAGCATTCATCCCAGGAGATTCAGATATTAGGCTAAGAGATATTAATCCTCAAACTGCGATACACCTATTACTAAGGGCTATATATATGCAGGTCACAAGGAATGCTATACGTGGAGATATCTTATTCCAGGATCCAAATGTATAA

Protein sequence

>Msa1264260

LDIAKGLSHYYIADYDVLHLREAFIPGDSDIRLRDINPQTAIHLLLRAIYMQVTRNAIRGDILFQDPNV*