AlfalfaGEDB | Msa1268440

M. sativa cultivar zhongmu-4 / Msa1268440

KEGG: K00434
GO: GO:0000302 GO:0003674 GO:0003824 GO:0004130 GO:0004601 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005774 GO:0005777 GO:0005778 GO:0005911 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009506 GO:0009507 GO:0009526 GO:0009536 GO:0009636 GO:0009719 GO:0009725 GO:0009735 GO:0009941 GO:0009987 GO:0010033 GO:0016020 GO:0016209 GO:0016491 GO:0016684 GO:0016688 GO:0016999 GO:0017001 GO:0017144 GO:0030054 GO:0031090 GO:0031903 GO:0031967 GO:0031975 GO:0033554 GO:0034599 GO:0042221 GO:0042579 GO:0042737 GO:0042743 GO:0042744 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044248 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051186 GO:0051187 GO:0051716 GO:0055044 GO:0055114 GO:0070887 GO:0072593 GO:0097237 GO:0098588 GO:0098754 GO:0098805 GO:0098869 GO:1901700 GO:1990748
PFAM: PF00141.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1268440	sp\|Q42564\|APX3_ARATH	66.160	263	85	3	1	263	1	259	4.44e-122	353

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1268440	G7JXJ2	87.368	285	32	2	1	285	1	281	3.88e-177	499

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0015150	Msa1268440	0.820987	5.128749e-53	-8.615850e-47
Msa0155180	Msa1268440	0.806395	8.400359e-50	-8.615850e-47
Msa0432320	Msa1268440	0.800520	1.386964e-48	-8.615850e-47
Msa0609300	Msa1268440	0.841572	4.379710e-58	-8.615850e-47
Msa0691910	Msa1268440	0.837225	5.894510e-57	-8.615850e-47
Msa0759010	Msa1268440	0.944011	4.256377e-103	-8.615850e-47
Msa0800140	Msa1268440	0.965851	3.829314e-125	-8.615850e-47
Msa0800170	Msa1268440	0.958670	1.324062e-116	-8.615850e-47
Msa0800200	Msa1268440	0.969855	9.694694e-131	-8.615850e-47
Msa1268380	Msa1268440	0.980652	1.023298e-150	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1268440	MtrunA17_Chr5g0405731	87.368	285	32	2	1	285	1	281	0.0	499
Msa1268440	MtrunA17_Chr4g0037131	65.385	286	84	4	6	283	7	285	8.91e-128	365
Msa1268440	MtrunA17_Chr4g0030171	57.317	246	96	3	6	248	8	247	6.45e-95	280
Msa1268440	MtrunA17_Chr3g0124821	44.444	261	122	5	13	250	100	360	8.80e-66	210
Msa1268440	MtrunA17_Chr3g0137751	32.794	247	138	8	11	247	86	314	7.32e-28	109
Msa1268440	MtrunA17_Chr5g0426581	44.706	85	41	1	27	105	113	197	1.27e-12	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1268440	AT4G35000.1	66.160	263	85	3	1	263	1	259	4.51e-123	353
Msa1268440	AT4G35970.1	61.511	278	102	2	6	283	5	277	5.43e-120	345
Msa1268440	AT4G35970.2	65.315	222	72	2	6	227	5	221	2.16e-100	293
Msa1268440	AT3G09640.2	56.327	245	99	2	6	248	9	247	2.95e-91	271
Msa1268440	AT3G09640.1	56.327	245	99	2	6	248	9	247	2.95e-91	271
Msa1268440	AT1G07890.2	53.279	244	109	4	6	248	8	247	7.54e-86	257
Msa1268440	AT1G07890.8	53.279	244	109	4	6	248	8	247	7.54e-86	257
Msa1268440	AT1G07890.4	53.279	244	109	4	6	248	8	247	7.54e-86	257
Msa1268440	AT1G07890.7	53.279	244	109	4	6	248	8	247	7.54e-86	257
Msa1268440	AT1G07890.1	53.279	244	109	4	6	248	8	247	7.54e-86	257

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Find 71 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGCTGTTGAATTATG+TGG	0.483315	8_3:-1437988	Msa1268440:intron
AAACAGGGAACTAATGAAAT+TGG	0.251753	8_3:-1436724	Msa1268440:intron
AAAGACATTGTAGCTCTCTC+CGG	0.548124	8_3:-1437018	Msa1268440:CDS
AAAGATCGCTCTAACTTTGA+AGG	0.430021	8_3:-1436870	Msa1268440:CDS
AAGAGTTATCAAACTTGAGA+GGG	0.589980	8_3:+1436832	None:intergenic
AAGGTCCGCATATGAAACTT+TGG	0.433809	8_3:+1437830	None:intergenic
AATTAATTTGTACCAAAGTG+TGG	0.573092	8_3:+1436992	None:intergenic
ACACCCCAAAGTTTCATATG+CGG	0.685445	8_3:-1437835	Msa1268440:CDS
ACCCAGGATCACAACAGTTG+AGG	0.637431	8_3:+1436362	None:intergenic
AGAGTATGTTAAAGAAATCG+AGG	0.648519	8_3:-1438266	Msa1268440:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTTATGATTATGAAAGGGT+TGG	+	chr8_3:1436174-1436193	None:intergenic	30.0%
AGAGTATGTTAAAGAAATCG+AGG	-	chr8_3:1436258-1436277	Msa1268440:three_prime_UTR	30.0%
TGATTCCTTATAGAACCATT+TGG	+	chr8_3:1436482-1436501	None:intergenic	30.0%
AAAACAGCTGTTGAATTATG+TGG	-	chr8_3:1436536-1436555	Msa1268440:intron	30.0%
TTATATGTATTTCAGCTAGC+TGG	-	chr8_3:1437104-1437123	Msa1268440:intron	30.0%
TATATGTATTTCAGCTAGCT+GGG	-	chr8_3:1437105-1437124	Msa1268440:intron	30.0%
CATTTCAATTGATAAGGGTA+AGG	-	chr8_3:1437626-1437645	Msa1268440:intron	30.0%
AAGAGTTATCAAACTTGAGA+GGG	+	chr8_3:1437695-1437714	None:intergenic	30.0%
TAAGAGTTATCAAACTTGAG+AGG	+	chr8_3:1437696-1437715	None:intergenic	30.0%
AAACAGGGAACTAATGAAAT+TGG	-	chr8_3:1437800-1437819	Msa1268440:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_3	gene	1436139	1438407	1436139	ID=Msa1268440;Name=Msa1268440
chr8_3	mRNA	1436139	1438407	1436139	ID=Msa1268440-mRNA-1;Parent=Msa1268440;Name=Msa1268440-mRNA-1;_AED=0.14;_eAED=0.14;_QI=96\|0.87\|0.88\|1\|0.62\|0.77\|9\|190\|285
chr8_3	exon	1436139	1436505	1436139	ID=Msa1268440-mRNA-1:exon:409;Parent=Msa1268440-mRNA-1
chr8_3	exon	1436638	1436740	1436638	ID=Msa1268440-mRNA-1:exon:408;Parent=Msa1268440-mRNA-1
chr8_3	exon	1436825	1436904	1436825	ID=Msa1268440-mRNA-1:exon:407;Parent=Msa1268440-mRNA-1
chr8_3	exon	1437005	1437087	1437005	ID=Msa1268440-mRNA-1:exon:406;Parent=Msa1268440-mRNA-1
chr8_3	exon	1437167	1437228	1437167	ID=Msa1268440-mRNA-1:exon:405;Parent=Msa1268440-mRNA-1
chr8_3	exon	1437363	1437427	1437363	ID=Msa1268440-mRNA-1:exon:404;Parent=Msa1268440-mRNA-1
chr8_3	exon	1437824	1437873	1437824	ID=Msa1268440-mRNA-1:exon:403;Parent=Msa1268440-mRNA-1
chr8_3	exon	1437989	1438113	1437989	ID=Msa1268440-mRNA-1:exon:402;Parent=Msa1268440-mRNA-1
chr8_3	exon	1438199	1438407	1438199	ID=Msa1268440-mRNA-1:exon:401;Parent=Msa1268440-mRNA-1
chr8_3	five_prime_UTR	1438312	1438407	1438312	ID=Msa1268440-mRNA-1:five_prime_utr;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1438199	1438311	1438199	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1437989	1438113	1437989	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1437824	1437873	1437824	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1437363	1437427	1437363	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1437167	1437228	1437167	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1437005	1437087	1437005	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1436825	1436904	1436825	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1436638	1436740	1436638	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	CDS	1436329	1436505	1436329	ID=Msa1268440-mRNA-1:cds;Parent=Msa1268440-mRNA-1
chr8_3	three_prime_UTR	1436139	1436328	1436139	ID=Msa1268440-mRNA-1:three_prime_utr;Parent=Msa1268440-mRNA-1

Gene Sequence

>Msa1268440

ATGGCTAAATATGCAGTTGATGCAGAGTATGTTAAAGAAATCGAGGAGGCTCGTCGTGATCTTCGTTCTCTTATCAACAATAAAAAATGTGCTCCTCTCATGCTACGATTAGCGTGGCATGATGCTGGTACATACGACGCAAAAACAAGAACAGGGGGTCCAAATGGTTCTATAAGGAATCAACAAGAGCTGAACCATGCTGCTAACAAGGGCTTGAAAACAGCTGTTGAATTATGTGAGGAAGTGAAGGTTAAACACCCCAAAGTTTCATATGCGGACCTTTACCAGCTAGCTGGGGTTGTGGCTGTAGAGGTCACTAGGGTCCAACTATTCACTTTGTTCCCGGAAGAAAGGATTCATTGGAATCTCCACAAGAAGGTCGCCTTCCAGATGCCAAACAAGGTAATTAAATCATGTGCATCACATTTAAGAGAGATCTTTTATCGCATGGGTCTCACTGATAAAGACATTGTAGCTCTCTCCGGAGGCCACACTTTGGGTAAGGCACATAAAGATCGCTCTAACTTTGAAGGTCAATGGACTAGAGACCCTCTCAAGTTTGATAACTCTTATTTTGTGGAACTAATGAAATTGGAATCAAAAGATTTATTGAAGCTTCCTACAGACAAGGCTCTAGTTGATGATCCTGCATTCCGCAAATATGTTGAACTCTATGCTAAGGATGAGAATGCATTTTTCAGAGATTATGCAACATCACACAAGAAACTCTCTGAATTAGGCTTCAATCCCGGTGGCAGTTATCGTTCTCAATTGGCTAAGGCAGCGTTAGGAATGGTTATTGCCTCAACTGTTGTGATCCTGGGTTACTTACTTGAGCTCAACAAAAAAATCAATTGA

Protein sequence

>Msa1268440

MAKYAVDAEYVKEIEEARRDLRSLINNKKCAPLMLRLAWHDAGTYDAKTRTGGPNGSIRNQQELNHAANKGLKTAVELCEEVKVKHPKVSYADLYQLAGVVAVEVTRVQLFTLFPEERIHWNLHKKVAFQMPNKVIKSCASHLREIFYRMGLTDKDIVALSGGHTLGKAHKDRSNFEGQWTRDPLKFDNSYFVELMKLESKDLLKLPTDKALVDDPAFRKYVELYAKDENAFFRDYATSHKKLSELGFNPGGSYRSQLAKAALGMVIASTVVILGYLLELNKKIN*