AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0759010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0759010 MtrunA17_Chr5g0405731 97.059 136 4 0 1 136 146 281 5.74e-94 272
Msa0759010 MtrunA17_Chr4g0037131 66.906 139 41 1 1 134 147 285 5.04e-57 179
Msa0759010 MtrunA17_Chr4g0030171 68.687 99 31 0 1 99 149 247 3.28e-45 147
Msa0759010 MtrunA17_Chr3g0124821 52.101 119 39 3 1 101 242 360 1.90e-30 112
Msa0759010 MtrunA17_Chr1g0197411 33.562 146 54 5 3 109 185 326 7.29e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0759010 AT4G35970.1 62.687 134 50 0 1 134 144 277 8.40e-57 178
Msa0759010 AT4G35000.1 71.930 114 32 0 1 114 146 259 2.50e-55 175
Msa0759010 AT3G09640.2 68.687 99 31 0 1 99 149 247 8.40e-44 144
Msa0759010 AT3G09640.1 68.687 99 31 0 1 99 149 247 8.40e-44 144
Msa0759010 AT1G07890.2 69.697 99 30 0 1 99 149 247 9.02e-44 144
Msa0759010 AT1G07890.8 69.697 99 30 0 1 99 149 247 9.02e-44 144
Msa0759010 AT1G07890.4 69.697 99 30 0 1 99 149 247 9.02e-44 144
Msa0759010 AT1G07890.7 69.697 99 30 0 1 99 149 247 9.02e-44 144
Msa0759010 AT1G07890.1 69.697 99 30 0 1 99 149 247 9.02e-44 144
Msa0759010 AT1G07890.3 69.697 99 30 0 1 99 149 247 9.02e-44 144
Msa0759010 AT1G07890.5 69.697 99 30 0 1 99 149 247 9.02e-44 144
Msa0759010 AT1G07890.6 68.687 99 31 0 1 99 149 247 2.68e-43 143
Msa0759010 AT4G35970.2 74.359 78 20 0 1 78 144 221 1.21e-36 125
Msa0759010 AT4G08390.3 51.261 119 40 3 1 101 247 365 2.78e-30 112
Msa0759010 AT4G08390.2 51.261 119 40 3 1 101 248 366 2.97e-30 112
Msa0759010 AT4G08390.5 51.261 119 40 3 1 101 248 366 2.97e-30 112
Msa0759010 AT4G08390.1 51.261 119 40 3 1 101 248 366 2.97e-30 112
Msa0759010 AT1G77490.2 52.101 119 39 3 1 101 227 345 3.89e-29 109
Msa0759010 AT1G77490.1 52.101 119 39 3 1 101 227 345 4.54e-29 109
Msa0759010 AT4G08390.4 42.017 119 25 4 1 101 248 340 1.43e-17 77.4

Find 62 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATATAGTATTGATTTAT+AGG 0.225416 5_2:-14550387 Msa0759010:five_prime_UTR
AAACAGGGAACTAATGAAAT+TGG 0.251753 5_2:-14548993 Msa0759010:intron
TGCCTCAACTGTTGTGATCC+TGG 0.267857 5_2:-14548631 Msa0759010:CDS
CATGATTTAATTACCTTGTT+TGG 0.286806 5_2:+14549437 None:intergenic
CACAAGAAACTCTCTGAATT+AGG 0.339734 5_2:-14548714 Msa0759010:CDS
ATGTACTAGTACCTTTCTTC+CGG 0.351786 5_2:+14549622 None:intergenic
CCAACTATTCACTTTGTTCC+CGG 0.389997 5_2:-14549641 Msa0759010:five_prime_UTR
AGGTCCGCATATGAAACTTT+GGG 0.390870 5_2:+14550104 None:intergenic
TTATATGTATTTCAGCTAGC+TGG 0.393811 5_2:-14549692 Msa0759010:intron
TAATTACCTTGTTTGGCATC+TGG 0.400655 5_2:+14549444 None:intergenic
TGATTCCTTATAGAACCATT+TGG 0.401526 5_2:+14550319 None:intergenic
GCCTCAACTGTTGTGATCCT+GGG 0.428816 5_2:-14548630 Msa0759010:CDS
AAAGATCGCTCTAACTTTGA+AGG 0.430021 5_2:-14549139 Msa0759010:CDS
AAGGTCCGCATATGAAACTT+TGG 0.433809 5_2:+14550103 None:intergenic
TGTACTAGTACCTTTCTTCC+GGG 0.435662 5_2:+14549623 None:intergenic
TGGCAGTTATCGTTCTCAAT+TGG 0.438208 5_2:-14548679 Msa0759010:CDS
CATAGAGTTCAACATATTTG+CGG 0.439517 5_2:+14548912 None:intergenic
TCAACATATTTGCGGAATGC+AGG 0.460562 5_2:+14548920 None:intergenic
TCACTTTGTTCCCGGAAGAA+AGG 0.460671 5_2:-14549633 Msa0759010:intron
TATATGTATTTCAGCTAGCT+GGG 0.463788 5_2:-14549691 Msa0759010:intron
CAATTGGCTAAGGCAGCGTT+AGG 0.473147 5_2:-14548663 Msa0759010:CDS
GTTGTGGCTGTAGAGGTCAC+TGG 0.475048 5_2:-14549668 Msa0759010:five_prime_UTR
AAAACAGCTGTTGAATTATG+TGG 0.483315 5_2:-14550262 Msa0759010:intron
AGCTGGGGTTGTGGCTGTAG+AGG 0.496133 5_2:-14549675 Msa0759010:five_prime_UTR
GCTCTAACTTTGAAGGTCAA+TGG 0.497264 5_2:-14549132 Msa0759010:CDS
GGGGTCCAAATGGTTCTATA+AGG 0.497436 5_2:-14550324 Msa0759010:five_prime_UTR
CCGGGAACAAAGTGAATAGT+TGG 0.498623 5_2:+14549641 None:intergenic
GCTGAACCATGCTGCTAACA+AGG 0.508818 5_2:-14550290 Msa0759010:five_prime_UTR
ATTTCAGCTAGCTGGGGTTG+TGG 0.511946 5_2:-14549684 Msa0759010:intron
ATTATAAAATAACCTGGTAA+AGG 0.515916 5_2:+14550084 None:intergenic
TTCATATGCGGACCTTTACC+AGG 0.516618 5_2:-14550096 Msa0759010:intron
TCTGGAAGGCGACCTTCTTG+TGG 0.517530 5_2:+14549462 None:intergenic
TTGAGAAACAGAGGAAGTGA+AGG 0.521165 5_2:-14550135 Msa0759010:intron
TCAAGCCCTTGTTAGCAGCA+TGG 0.531740 5_2:+14550284 None:intergenic
ATATGTTGAACTCTATGCTA+AGG 0.543090 5_2:-14548906 Msa0759010:intron
TTGTACCAAAGTGTGGCCTC+CGG 0.545189 5_2:+14549270 None:intergenic
AAAGACATTGTAGCTCTCTC+CGG 0.548124 5_2:-14549289 Msa0759010:CDS
CGCCTTCCAGATGCCAAACA+AGG 0.549529 5_2:-14549450 Msa0759010:intron
TTATCGTTCTCAATTGGCTA+AGG 0.549693 5_2:-14548673 Msa0759010:CDS
ATTTATAGGTGGCATGATGC+TGG 0.555051 5_2:-14550373 Msa0759010:five_prime_UTR
TACCTTGTTTGGCATCTGGA+AGG 0.559846 5_2:+14549448 None:intergenic
AATTAATTTGTACCAAAGTG+TGG 0.573092 5_2:+14549263 None:intergenic
GGCTAAGGCAGCGTTAGGAA+TGG 0.573545 5_2:-14548658 Msa0759010:CDS
TAAGAGTTATCAAACTTGAG+AGG 0.573775 5_2:+14549100 None:intergenic
TTGAGAACGATAACTGCCAC+CGG 0.582999 5_2:+14548683 None:intergenic
AAGAGTTATCAAACTTGAGA+GGG 0.589980 5_2:+14549101 None:intergenic
TCTCTCCGGAGGCCACACTT+TGG 0.591661 5_2:-14549275 Msa0759010:intron
TCTGAATTAGGCTTCAATCC+CGG 0.595170 5_2:-14548702 Msa0759010:CDS
GGTCCGCATATGAAACTTTG+GGG 0.601066 5_2:+14550105 None:intergenic
TCAACTAGAGCCTTGTCTGT+AGG 0.607702 5_2:+14548947 None:intergenic
ATTGAAGCTTCCTACAGACA+AGG 0.615401 5_2:-14548957 Msa0759010:CDS
CTGAACCATGCTGCTAACAA+GGG 0.615953 5_2:-14550289 Msa0759010:five_prime_UTR
GACATTGTAGCTCTCTCCGG+AGG 0.618468 5_2:-14549286 Msa0759010:CDS
ATATAGTATTGATTTATAGG+TGG 0.634896 5_2:-14550384 Msa0759010:five_prime_UTR
TCATTGGAATCTCCACAAGA+AGG 0.637304 5_2:-14549474 Msa0759010:five_prime_UTR
ACCCAGGATCACAACAGTTG+AGG 0.637431 5_2:+14548629 None:intergenic
ATATGTATTTCAGCTAGCTG+GGG 0.644095 5_2:-14549690 Msa0759010:intron
TGAGCTCAAGTAAGTAACCC+AGG 0.647120 5_2:+14548613 None:intergenic
ACACCCCAAAGTTTCATATG+CGG 0.685445 5_2:-14550108 Msa0759010:five_prime_UTR
TGAGAACGATAACTGCCACC+GGG 0.688112 5_2:+14548684 None:intergenic
GTGGCTGTAGAGGTCACTGG+TGG 0.707904 5_2:-14549665 Msa0759010:five_prime_UTR
GAATTAGGCTTCAATCCCGG+TGG 0.713172 5_2:-14548699 Msa0759010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATATAGTATTGATTTAT+AGG - chr5_2:14548449-14548468 Msa0759010:three_prime_UTR 10.0%
!! AAAAATATTATAAAATAACC+TGG + chr5_2:14548761-14548780 None:intergenic 10.0%
!!! TCTCTATTTTTTTTTTTTTT+TGG - chr5_2:14549981-14550000 Msa0759010:intron 10.0%
!!! AATATATAGTATTGATTTAT+AGG - chr5_2:14548449-14548468 Msa0759010:three_prime_UTR 10.0%
!! AAAAATATTATAAAATAACC+TGG + chr5_2:14548761-14548780 None:intergenic 10.0%
!!! TCTCTATTTTTTTTTTTTTT+TGG - chr5_2:14549981-14550000 Msa0759010:intron 10.0%
!! AAGATTAATAAGCAAAAAAT+TGG + chr5_2:14548659-14548678 None:intergenic 15.0%
!!! TTAATTCATATTCAATTTTG+TGG + chr5_2:14549015-14549034 None:intergenic 15.0%
!! ATTATTATAGTAGTATTATG+AGG - chr5_2:14549318-14549337 Msa0759010:exon 15.0%
!! TGTATAAAAAACTATATTTC+AGG - chr5_2:14549478-14549497 Msa0759010:five_prime_UTR 15.0%
!! TTCTTCAAGAAATATAATAA+AGG + chr5_2:14549624-14549643 None:intergenic 15.0%
!!! ATAGTATTATGTATTTTTTG+TGG - chr5_2:14550037-14550056 Msa0759010:intron 15.0%
!!! TAATGATTTTGAATTTTACT+AGG - chr5_2:14550064-14550083 Msa0759010:intron 15.0%
!! AAGATTAATAAGCAAAAAAT+TGG + chr5_2:14548659-14548678 None:intergenic 15.0%
!!! TTAATTCATATTCAATTTTG+TGG + chr5_2:14549015-14549034 None:intergenic 15.0%
!! ATTATTATAGTAGTATTATG+AGG - chr5_2:14549318-14549337 Msa0759010:exon 15.0%
!! TGTATAAAAAACTATATTTC+AGG - chr5_2:14549478-14549497 Msa0759010:five_prime_UTR 15.0%
!! TTCTTCAAGAAATATAATAA+AGG + chr5_2:14549624-14549643 None:intergenic 15.0%
!!! ATAGTATTATGTATTTTTTG+TGG - chr5_2:14550037-14550056 Msa0759010:intron 15.0%
!!! TAATGATTTTGAATTTTACT+AGG - chr5_2:14550064-14550083 Msa0759010:intron 15.0%
!!! ATATAGTATTGATTTATAGG+TGG - chr5_2:14548452-14548471 Msa0759010:three_prime_UTR 20.0%
!! ATTATAAAATAACCTGGTAA+AGG + chr5_2:14548755-14548774 None:intergenic 20.0%
!! AATTCACTTGAATAAATGAT+TGG - chr5_2:14548911-14548930 Msa0759010:CDS 20.0%
!! CTAGATTAAGTTAAAGATTA+TGG - chr5_2:14548939-14548958 Msa0759010:CDS 20.0%
!! ATTAATCGTGTGTAAAAATA+TGG + chr5_2:14549065-14549084 None:intergenic 20.0%
!!! TATTTTTACACACGATTAAT+CGG - chr5_2:14549065-14549084 Msa0759010:intron 20.0%
!!! TATTTTGAATTAGGATTCAT+TGG - chr5_2:14549346-14549365 Msa0759010:intron 20.0%
!! TCATTCATTTCAATTGATAA+GGG - chr5_2:14549664-14549683 Msa0759010:five_prime_UTR 20.0%
!! AATACAAGATTAACATAAAC+AGG - chr5_2:14549827-14549846 Msa0759010:intron 20.0%
!! ATACAAGATTAACATAAACA+GGG - chr5_2:14549828-14549847 Msa0759010:intron 20.0%
!!! ATGATGATATGTTTTTTATG+AGG + chr5_2:14550338-14550357 None:intergenic 20.0%
!!! ATATAGTATTGATTTATAGG+TGG - chr5_2:14548452-14548471 Msa0759010:three_prime_UTR 20.0%
!! ATTATAAAATAACCTGGTAA+AGG + chr5_2:14548755-14548774 None:intergenic 20.0%
!! AATTCACTTGAATAAATGAT+TGG - chr5_2:14548911-14548930 Msa0759010:CDS 20.0%
!! CTAGATTAAGTTAAAGATTA+TGG - chr5_2:14548939-14548958 Msa0759010:CDS 20.0%
!! ATTAATCGTGTGTAAAAATA+TGG + chr5_2:14549065-14549084 None:intergenic 20.0%
!!! TATTTTTACACACGATTAAT+CGG - chr5_2:14549065-14549084 Msa0759010:intron 20.0%
!!! TATTTTGAATTAGGATTCAT+TGG - chr5_2:14549346-14549365 Msa0759010:intron 20.0%
!! TCATTCATTTCAATTGATAA+GGG - chr5_2:14549664-14549683 Msa0759010:five_prime_UTR 20.0%
!! AATACAAGATTAACATAAAC+AGG - chr5_2:14549827-14549846 Msa0759010:intron 20.0%
!! ATACAAGATTAACATAAACA+GGG - chr5_2:14549828-14549847 Msa0759010:intron 20.0%
!!! ATGATGATATGTTTTTTATG+AGG + chr5_2:14550338-14550357 None:intergenic 20.0%
!!! TTTTTTGTTTTGAGAAACAG+AGG - chr5_2:14548692-14548711 Msa0759010:CDS 25.0%
! ATAATCAAGATATTAACGTG+TGG - chr5_2:14548794-14548813 Msa0759010:intron 25.0%
! TTATGTACAAAAATGTTTCG+TGG - chr5_2:14548832-14548851 Msa0759010:intron 25.0%
! CATAATCAACTGCAAAAATA+TGG - chr5_2:14548886-14548905 Msa0759010:intron 25.0%
!!! ATGTAGTTTGTTTTTGTCAA+AGG - chr5_2:14548968-14548987 Msa0759010:CDS 25.0%
! TAGATTGTTACTAAATCATG+AGG + chr5_2:14549288-14549307 None:intergenic 25.0%
!!! GAGGTGTTTTATTTTGAATT+AGG - chr5_2:14549337-14549356 Msa0759010:five_prime_UTR 25.0%
! CATGATTTAATTACCTTGTT+TGG + chr5_2:14549402-14549421 None:intergenic 25.0%
!!! AATTAATTTGTACCAAAGTG+TGG + chr5_2:14549576-14549595 None:intergenic 25.0%
! AGTGTTTGAATTATTATGGT+TGG + chr5_2:14549600-14549619 None:intergenic 25.0%
!!! AGGAAGTGTTTGAATTATTA+TGG + chr5_2:14549604-14549623 None:intergenic 25.0%
! CTCATTCATTTCAATTGATA+AGG - chr5_2:14549663-14549682 Msa0759010:five_prime_UTR 25.0%
!!! TTTTTTGTTTTGAGAAACAG+AGG - chr5_2:14548692-14548711 Msa0759010:CDS 25.0%
! ATAATCAAGATATTAACGTG+TGG - chr5_2:14548794-14548813 Msa0759010:intron 25.0%
! TTATGTACAAAAATGTTTCG+TGG - chr5_2:14548832-14548851 Msa0759010:intron 25.0%
! CATAATCAACTGCAAAAATA+TGG - chr5_2:14548886-14548905 Msa0759010:intron 25.0%
!!! ATGTAGTTTGTTTTTGTCAA+AGG - chr5_2:14548968-14548987 Msa0759010:CDS 25.0%
! TAGATTGTTACTAAATCATG+AGG + chr5_2:14549288-14549307 None:intergenic 25.0%
!!! GAGGTGTTTTATTTTGAATT+AGG - chr5_2:14549337-14549356 Msa0759010:five_prime_UTR 25.0%
! CATGATTTAATTACCTTGTT+TGG + chr5_2:14549402-14549421 None:intergenic 25.0%
!!! AATTAATTTGTACCAAAGTG+TGG + chr5_2:14549576-14549595 None:intergenic 25.0%
! AGTGTTTGAATTATTATGGT+TGG + chr5_2:14549600-14549619 None:intergenic 25.0%
!!! AGGAAGTGTTTGAATTATTA+TGG + chr5_2:14549604-14549623 None:intergenic 25.0%
! CTCATTCATTTCAATTGATA+AGG - chr5_2:14549663-14549682 Msa0759010:five_prime_UTR 25.0%
TGATTCCTTATAGAACCATT+TGG + chr5_2:14548520-14548539 None:intergenic 30.0%
AAAACAGCTGTTGAATTATG+TGG - chr5_2:14548574-14548593 Msa0759010:three_prime_UTR 30.0%
TTATATGTATTTCAGCTAGC+TGG - chr5_2:14549144-14549163 Msa0759010:CDS 30.0%
TATATGTATTTCAGCTAGCT+GGG - chr5_2:14549145-14549164 Msa0759010:CDS 30.0%
CATTTCAATTGATAAGGGTA+AGG - chr5_2:14549669-14549688 Msa0759010:five_prime_UTR 30.0%
AAGAGTTATCAAACTTGAGA+GGG + chr5_2:14549738-14549757 None:intergenic 30.0%
TAAGAGTTATCAAACTTGAG+AGG + chr5_2:14549739-14549758 None:intergenic 30.0%
AAACAGGGAACTAATGAAAT+TGG - chr5_2:14549843-14549862 Msa0759010:intron 30.0%
CATAGAGTTCAACATATTTG+CGG + chr5_2:14549927-14549946 None:intergenic 30.0%
ATATGTTGAACTCTATGCTA+AGG - chr5_2:14549930-14549949 Msa0759010:intron 30.0%
TGATTCCTTATAGAACCATT+TGG + chr5_2:14548520-14548539 None:intergenic 30.0%
AAAACAGCTGTTGAATTATG+TGG - chr5_2:14548574-14548593 Msa0759010:three_prime_UTR 30.0%
TTATATGTATTTCAGCTAGC+TGG - chr5_2:14549144-14549163 Msa0759010:CDS 30.0%
TATATGTATTTCAGCTAGCT+GGG - chr5_2:14549145-14549164 Msa0759010:CDS 30.0%
CATTTCAATTGATAAGGGTA+AGG - chr5_2:14549669-14549688 Msa0759010:five_prime_UTR 30.0%
AAGAGTTATCAAACTTGAGA+GGG + chr5_2:14549738-14549757 None:intergenic 30.0%
TAAGAGTTATCAAACTTGAG+AGG + chr5_2:14549739-14549758 None:intergenic 30.0%
AAACAGGGAACTAATGAAAT+TGG - chr5_2:14549843-14549862 Msa0759010:intron 30.0%
CATAGAGTTCAACATATTTG+CGG + chr5_2:14549927-14549946 None:intergenic 30.0%
ATATGTTGAACTCTATGCTA+AGG - chr5_2:14549930-14549949 Msa0759010:intron 30.0%
ATATGTATTTCAGCTAGCTG+GGG - chr5_2:14549146-14549165 Msa0759010:CDS 35.0%
ATGTACTAGTACCTTTCTTC+CGG + chr5_2:14549217-14549236 None:intergenic 35.0%
! TAATTACCTTGTTTGGCATC+TGG + chr5_2:14549395-14549414 None:intergenic 35.0%
! AAGAGAGATCTTTTATCGCA+TGG - chr5_2:14549513-14549532 Msa0759010:intron 35.0%
! AGAGAGATCTTTTATCGCAT+GGG - chr5_2:14549514-14549533 Msa0759010:intron 35.0%
AAAGATCGCTCTAACTTTGA+AGG - chr5_2:14549697-14549716 Msa0759010:intron 35.0%
CACAAGAAACTCTCTGAATT+AGG - chr5_2:14550122-14550141 Msa0759010:five_prime_UTR 35.0%
TTATCGTTCTCAATTGGCTA+AGG - chr5_2:14550163-14550182 Msa0759010:intron 35.0%
ATATGTATTTCAGCTAGCTG+GGG - chr5_2:14549146-14549165 Msa0759010:CDS 35.0%
ATGTACTAGTACCTTTCTTC+CGG + chr5_2:14549217-14549236 None:intergenic 35.0%
! TAATTACCTTGTTTGGCATC+TGG + chr5_2:14549395-14549414 None:intergenic 35.0%
! AAGAGAGATCTTTTATCGCA+TGG - chr5_2:14549513-14549532 Msa0759010:intron 35.0%
! AGAGAGATCTTTTATCGCAT+GGG - chr5_2:14549514-14549533 Msa0759010:intron 35.0%
AAAGATCGCTCTAACTTTGA+AGG - chr5_2:14549697-14549716 Msa0759010:intron 35.0%
CACAAGAAACTCTCTGAATT+AGG - chr5_2:14550122-14550141 Msa0759010:five_prime_UTR 35.0%
TTATCGTTCTCAATTGGCTA+AGG - chr5_2:14550163-14550182 Msa0759010:intron 35.0%
ATTTATAGGTGGCATGATGC+TGG - chr5_2:14548463-14548482 Msa0759010:three_prime_UTR 40.0%
TACGACGCAAAAACAAGAAC+AGG - chr5_2:14548490-14548509 Msa0759010:three_prime_UTR 40.0%
ACGACGCAAAAACAAGAACA+GGG - chr5_2:14548491-14548510 Msa0759010:three_prime_UTR 40.0%
GAATTATGTGGTCAGTGAGA+CGG - chr5_2:14548586-14548605 Msa0759010:exon 40.0%
TTGAGAAACAGAGGAAGTGA+AGG - chr5_2:14548701-14548720 Msa0759010:CDS 40.0%
ACACCCCAAAGTTTCATATG+CGG - chr5_2:14548728-14548747 Msa0759010:CDS 40.0%
AGGTCCGCATATGAAACTTT+GGG + chr5_2:14548735-14548754 None:intergenic 40.0%
AAGGTCCGCATATGAAACTT+TGG + chr5_2:14548736-14548755 None:intergenic 40.0%
GATATTAACGTGTGGAGTCA+TGG - chr5_2:14548802-14548821 Msa0759010:intron 40.0%
CCAACTATTCACTTTGTTCC+CGG - chr5_2:14549195-14549214 Msa0759010:intron 40.0%
TGTACTAGTACCTTTCTTCC+GGG + chr5_2:14549216-14549235 None:intergenic 40.0%
TCATTGGAATCTCCACAAGA+AGG - chr5_2:14549362-14549381 Msa0759010:intron 40.0%
AAAGACATTGTAGCTCTCTC+CGG - chr5_2:14549547-14549566 Msa0759010:intron 40.0%
! GCTCTAACTTTGAAGGTCAA+TGG - chr5_2:14549704-14549723 Msa0759010:intron 40.0%
ATTGAAGCTTCCTACAGACA+AGG - chr5_2:14549879-14549898 Msa0759010:intron 40.0%
TCAACATATTTGCGGAATGC+AGG + chr5_2:14549919-14549938 None:intergenic 40.0%
TCTGAATTAGGCTTCAATCC+CGG - chr5_2:14550134-14550153 Msa0759010:intron 40.0%
TGGCAGTTATCGTTCTCAAT+TGG - chr5_2:14550157-14550176 Msa0759010:intron 40.0%
ATTTATAGGTGGCATGATGC+TGG - chr5_2:14548463-14548482 Msa0759010:three_prime_UTR 40.0%
TACGACGCAAAAACAAGAAC+AGG - chr5_2:14548490-14548509 Msa0759010:three_prime_UTR 40.0%
ACGACGCAAAAACAAGAACA+GGG - chr5_2:14548491-14548510 Msa0759010:three_prime_UTR 40.0%
GAATTATGTGGTCAGTGAGA+CGG - chr5_2:14548586-14548605 Msa0759010:exon 40.0%
TTGAGAAACAGAGGAAGTGA+AGG - chr5_2:14548701-14548720 Msa0759010:CDS 40.0%
ACACCCCAAAGTTTCATATG+CGG - chr5_2:14548728-14548747 Msa0759010:CDS 40.0%
AGGTCCGCATATGAAACTTT+GGG + chr5_2:14548735-14548754 None:intergenic 40.0%
AAGGTCCGCATATGAAACTT+TGG + chr5_2:14548736-14548755 None:intergenic 40.0%
GATATTAACGTGTGGAGTCA+TGG - chr5_2:14548802-14548821 Msa0759010:intron 40.0%
CCAACTATTCACTTTGTTCC+CGG - chr5_2:14549195-14549214 Msa0759010:intron 40.0%
TGTACTAGTACCTTTCTTCC+GGG + chr5_2:14549216-14549235 None:intergenic 40.0%
TCATTGGAATCTCCACAAGA+AGG - chr5_2:14549362-14549381 Msa0759010:intron 40.0%
AAAGACATTGTAGCTCTCTC+CGG - chr5_2:14549547-14549566 Msa0759010:intron 40.0%
! GCTCTAACTTTGAAGGTCAA+TGG - chr5_2:14549704-14549723 Msa0759010:intron 40.0%
ATTGAAGCTTCCTACAGACA+AGG - chr5_2:14549879-14549898 Msa0759010:intron 40.0%
TCAACATATTTGCGGAATGC+AGG + chr5_2:14549919-14549938 None:intergenic 40.0%
TCTGAATTAGGCTTCAATCC+CGG - chr5_2:14550134-14550153 Msa0759010:intron 40.0%
TGGCAGTTATCGTTCTCAAT+TGG - chr5_2:14550157-14550176 Msa0759010:intron 40.0%
CGACGCAAAAACAAGAACAG+GGG - chr5_2:14548492-14548511 Msa0759010:three_prime_UTR 45.0%
GACGCAAAAACAAGAACAGG+GGG - chr5_2:14548493-14548512 Msa0759010:three_prime_UTR 45.0%
! GGGGTCCAAATGGTTCTATA+AGG - chr5_2:14548512-14548531 Msa0759010:three_prime_UTR 45.0%
CTGAACCATGCTGCTAACAA+GGG - chr5_2:14548547-14548566 Msa0759010:three_prime_UTR 45.0%
GGTCCGCATATGAAACTTTG+GGG + chr5_2:14548734-14548753 None:intergenic 45.0%
TTCATATGCGGACCTTTACC+AGG - chr5_2:14548740-14548759 Msa0759010:CDS 45.0%
CCGGGAACAAAGTGAATAGT+TGG + chr5_2:14549198-14549217 None:intergenic 45.0%
TCACTTTGTTCCCGGAAGAA+AGG - chr5_2:14549203-14549222 Msa0759010:intron 45.0%
! TACCTTGTTTGGCATCTGGA+AGG + chr5_2:14549391-14549410 None:intergenic 45.0%
! TCAACTAGAGCCTTGTCTGT+AGG + chr5_2:14549892-14549911 None:intergenic 45.0%
TTGAGAACGATAACTGCCAC+CGG + chr5_2:14550156-14550175 None:intergenic 45.0%
TGAGCTCAAGTAAGTAACCC+AGG + chr5_2:14550226-14550245 None:intergenic 45.0%
CGACGCAAAAACAAGAACAG+GGG - chr5_2:14548492-14548511 Msa0759010:three_prime_UTR 45.0%
GACGCAAAAACAAGAACAGG+GGG - chr5_2:14548493-14548512 Msa0759010:three_prime_UTR 45.0%
! GGGGTCCAAATGGTTCTATA+AGG - chr5_2:14548512-14548531 Msa0759010:three_prime_UTR 45.0%
CTGAACCATGCTGCTAACAA+GGG - chr5_2:14548547-14548566 Msa0759010:three_prime_UTR 45.0%
GGTCCGCATATGAAACTTTG+GGG + chr5_2:14548734-14548753 None:intergenic 45.0%
TTCATATGCGGACCTTTACC+AGG - chr5_2:14548740-14548759 Msa0759010:CDS 45.0%
CCGGGAACAAAGTGAATAGT+TGG + chr5_2:14549198-14549217 None:intergenic 45.0%
TCACTTTGTTCCCGGAAGAA+AGG - chr5_2:14549203-14549222 Msa0759010:intron 45.0%
! TACCTTGTTTGGCATCTGGA+AGG + chr5_2:14549391-14549410 None:intergenic 45.0%
! TCAACTAGAGCCTTGTCTGT+AGG + chr5_2:14549892-14549911 None:intergenic 45.0%
TTGAGAACGATAACTGCCAC+CGG + chr5_2:14550156-14550175 None:intergenic 45.0%
TGAGCTCAAGTAAGTAACCC+AGG + chr5_2:14550226-14550245 None:intergenic 45.0%
!! AAAATTAATTAAACAAATTA+AGG + chr5_2:14548618-14548637 None:intergenic 5.0%
!! AAAATTAATTAAACAAATTA+AGG + chr5_2:14548618-14548637 None:intergenic 5.0%
ACAAGAACAGGGGGTCCAAA+TGG - chr5_2:14548502-14548521 Msa0759010:three_prime_UTR 50.0%
GCTGAACCATGCTGCTAACA+AGG - chr5_2:14548546-14548565 Msa0759010:three_prime_UTR 50.0%
TCAAGCCCTTGTTAGCAGCA+TGG + chr5_2:14548555-14548574 None:intergenic 50.0%
TTATGTGGTCAGTGAGACGG+TGG - chr5_2:14548589-14548608 Msa0759010:exon 50.0%
TCCTCGCACGTGTGCTAAAA+AGG - chr5_2:14549088-14549107 Msa0759010:intron 50.0%
CCTCGCACGTGTGCTAAAAA+GGG - chr5_2:14549089-14549108 Msa0759010:intron 50.0%
ATTTCAGCTAGCTGGGGTTG+TGG - chr5_2:14549152-14549171 Msa0759010:CDS 50.0%
TTGTACCAAAGTGTGGCCTC+CGG + chr5_2:14549569-14549588 None:intergenic 50.0%
GAATTAGGCTTCAATCCCGG+TGG - chr5_2:14550137-14550156 Msa0759010:intron 50.0%
TGAGAACGATAACTGCCACC+GGG + chr5_2:14550155-14550174 None:intergenic 50.0%
CAATTGGCTAAGGCAGCGTT+AGG - chr5_2:14550173-14550192 Msa0759010:intron 50.0%
TGCCTCAACTGTTGTGATCC+TGG - chr5_2:14550205-14550224 Msa0759010:intron 50.0%
GCCTCAACTGTTGTGATCCT+GGG - chr5_2:14550206-14550225 Msa0759010:intron 50.0%
ACCCAGGATCACAACAGTTG+AGG + chr5_2:14550210-14550229 None:intergenic 50.0%
ACAAGAACAGGGGGTCCAAA+TGG - chr5_2:14548502-14548521 Msa0759010:three_prime_UTR 50.0%
GCTGAACCATGCTGCTAACA+AGG - chr5_2:14548546-14548565 Msa0759010:three_prime_UTR 50.0%
TCAAGCCCTTGTTAGCAGCA+TGG + chr5_2:14548555-14548574 None:intergenic 50.0%
TTATGTGGTCAGTGAGACGG+TGG - chr5_2:14548589-14548608 Msa0759010:exon 50.0%
TCCTCGCACGTGTGCTAAAA+AGG - chr5_2:14549088-14549107 Msa0759010:intron 50.0%
CCTCGCACGTGTGCTAAAAA+GGG - chr5_2:14549089-14549108 Msa0759010:intron 50.0%
ATTTCAGCTAGCTGGGGTTG+TGG - chr5_2:14549152-14549171 Msa0759010:CDS 50.0%
TTGTACCAAAGTGTGGCCTC+CGG + chr5_2:14549569-14549588 None:intergenic 50.0%
GAATTAGGCTTCAATCCCGG+TGG - chr5_2:14550137-14550156 Msa0759010:intron 50.0%
TGAGAACGATAACTGCCACC+GGG + chr5_2:14550155-14550174 None:intergenic 50.0%
CAATTGGCTAAGGCAGCGTT+AGG - chr5_2:14550173-14550192 Msa0759010:intron 50.0%
TGCCTCAACTGTTGTGATCC+TGG - chr5_2:14550205-14550224 Msa0759010:intron 50.0%
GCCTCAACTGTTGTGATCCT+GGG - chr5_2:14550206-14550225 Msa0759010:intron 50.0%
ACCCAGGATCACAACAGTTG+AGG + chr5_2:14550210-14550229 None:intergenic 50.0%
!! CCCTTTTTAGCACACGTGCG+AGG + chr5_2:14549092-14549111 None:intergenic 55.0%
GTTGTGGCTGTAGAGGTCAC+TGG - chr5_2:14549168-14549187 Msa0759010:intron 55.0%
TCTGGAAGGCGACCTTCTTG+TGG + chr5_2:14549377-14549396 None:intergenic 55.0%
CGCCTTCCAGATGCCAAACA+AGG - chr5_2:14549386-14549405 Msa0759010:intron 55.0%
GACATTGTAGCTCTCTCCGG+AGG - chr5_2:14549550-14549569 Msa0759010:intron 55.0%
GGCTAAGGCAGCGTTAGGAA+TGG - chr5_2:14550178-14550197 Msa0759010:intron 55.0%
!! CCCTTTTTAGCACACGTGCG+AGG + chr5_2:14549092-14549111 None:intergenic 55.0%
GTTGTGGCTGTAGAGGTCAC+TGG - chr5_2:14549168-14549187 Msa0759010:intron 55.0%
TCTGGAAGGCGACCTTCTTG+TGG + chr5_2:14549377-14549396 None:intergenic 55.0%
CGCCTTCCAGATGCCAAACA+AGG - chr5_2:14549386-14549405 Msa0759010:intron 55.0%
GACATTGTAGCTCTCTCCGG+AGG - chr5_2:14549550-14549569 Msa0759010:intron 55.0%
GGCTAAGGCAGCGTTAGGAA+TGG - chr5_2:14550178-14550197 Msa0759010:intron 55.0%
AGCTGGGGTTGTGGCTGTAG+AGG - chr5_2:14549161-14549180 Msa0759010:intron 60.0%
GTGGCTGTAGAGGTCACTGG+TGG - chr5_2:14549171-14549190 Msa0759010:intron 60.0%
TCTCTCCGGAGGCCACACTT+TGG - chr5_2:14549561-14549580 Msa0759010:intron 60.0%
AGCTGGGGTTGTGGCTGTAG+AGG - chr5_2:14549161-14549180 Msa0759010:intron 60.0%
GTGGCTGTAGAGGTCACTGG+TGG - chr5_2:14549171-14549190 Msa0759010:intron 60.0%
TCTCTCCGGAGGCCACACTT+TGG - chr5_2:14549561-14549580 Msa0759010:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 14548426 14550432 14548426 ID=Msa0759010;Name=Msa0759010
chr5_2 mRNA 14548426 14550432 14548426 ID=Msa0759010-mRNA-1;Parent=Msa0759010;Name=Msa0759010-mRNA-1;_AED=0.14;_eAED=0.14;_QI=367|1|1|1|1|1|8|170|136
chr5_2 exon 14548426 14548772 14548426 ID=Msa0759010-mRNA-1:exon:6635;Parent=Msa0759010-mRNA-1
chr5_2 exon 14548907 14549009 14548907 ID=Msa0759010-mRNA-1:exon:6634;Parent=Msa0759010-mRNA-1
chr5_2 exon 14549094 14549173 14549094 ID=Msa0759010-mRNA-1:exon:6633;Parent=Msa0759010-mRNA-1
chr5_2 exon 14549276 14549358 14549276 ID=Msa0759010-mRNA-1:exon:6632;Parent=Msa0759010-mRNA-1
chr5_2 exon 14549451 14549499 14549451 ID=Msa0759010-mRNA-1:exon:6631;Parent=Msa0759010-mRNA-1
chr5_2 exon 14549634 14549699 14549634 ID=Msa0759010-mRNA-1:exon:6630;Parent=Msa0759010-mRNA-1
chr5_2 exon 14550097 14550146 14550097 ID=Msa0759010-mRNA-1:exon:6629;Parent=Msa0759010-mRNA-1
chr5_2 exon 14550263 14550432 14550263 ID=Msa0759010-mRNA-1:exon:6628;Parent=Msa0759010-mRNA-1
chr5_2 five_prime_UTR 14550263 14550432 14550263 ID=Msa0759010-mRNA-1:five_prime_utr;Parent=Msa0759010-mRNA-1
chr5_2 five_prime_UTR 14550097 14550146 14550097 ID=Msa0759010-mRNA-1:five_prime_utr;Parent=Msa0759010-mRNA-1
chr5_2 five_prime_UTR 14549634 14549699 14549634 ID=Msa0759010-mRNA-1:five_prime_utr;Parent=Msa0759010-mRNA-1
chr5_2 five_prime_UTR 14549451 14549499 14549451 ID=Msa0759010-mRNA-1:five_prime_utr;Parent=Msa0759010-mRNA-1
chr5_2 five_prime_UTR 14549327 14549358 14549327 ID=Msa0759010-mRNA-1:five_prime_utr;Parent=Msa0759010-mRNA-1
chr5_2 CDS 14549276 14549326 14549276 ID=Msa0759010-mRNA-1:cds;Parent=Msa0759010-mRNA-1
chr5_2 CDS 14549094 14549173 14549094 ID=Msa0759010-mRNA-1:cds;Parent=Msa0759010-mRNA-1
chr5_2 CDS 14548907 14549009 14548907 ID=Msa0759010-mRNA-1:cds;Parent=Msa0759010-mRNA-1
chr5_2 CDS 14548596 14548772 14548596 ID=Msa0759010-mRNA-1:cds;Parent=Msa0759010-mRNA-1
chr5_2 three_prime_UTR 14548426 14548595 14548426 ID=Msa0759010-mRNA-1:three_prime_utr;Parent=Msa0759010-mRNA-1
Gene Sequence

>Msa0759010

ATGGGTCTCACTGATAAAGACATTGTAGCTCTCTCCGGAGGCCACACTTTGGGTAAGGCACATAAAGATCGCTCTAACTTTGAAGGTCAATGGACTAGAGACCCTCTCAAGTTTGATAACTCTTATTTTGTGGAACTAATGAAATTGGAATCAAAAGATTTATTGAAGCTTCCTACAGACAAGGCTCTAGTTGATGATCCTGCATTCCGCAAATATGTTGAACTCTATGCTAAGGATGAGAATGCATTTTTCAGAGATTATGCAACATCACACAAGAAACTCTCTGAATTAGGCTTCAATCCCGGTGGCAGTTATCGTTCTCAATTGGCTAAGGCAGCGTTAGGAATGGTTATTGCCTCAACTGTTGTGATCCTGGGTTACTTACTTGAGCTCAACAAAAAAATCAATTGA
Protein sequence

>Msa0759010

MGLTDKDIVALSGGHTLGKAHKDRSNFEGQWTRDPLKFDNSYFVELMKLESKDLLKLPTDKALVDDPAFRKYVELYAKDENAFFRDYATSHKKLSELGFNPGGSYRSQLAKAALGMVIASTVVILGYLLELNKKIN*