AlfalfaGEDB | Msa0609300

M. sativa cultivar zhongmu-4 / Msa0609300

KEGG: -
GO: -
PFAM: PF02298.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609300	XP_003607678.2	96.847	222	5	2	1	220	1	222	3.12e-154	436

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609300	sp\|Q9SK27\|ENL1_ARATH	47.899	119	61	1	18	135	17	135	8.52e-34	122

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609300	G7JF09	96.847	222	5	2	1	220	1	222	1.49e-154	436

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003740	Msa0609300	0.819992	8.676275e-53	-8.615850e-47
Msa0015150	Msa0609300	0.899194	2.522523e-77	-8.615850e-47
Msa0029960	Msa0609300	0.823918	1.069169e-53	-8.615850e-47
Msa0029970	Msa0609300	0.817560	3.095202e-52	-8.615850e-47
Msa0064120	Msa0609300	0.868462	6.413230e-66	-8.615850e-47
Msa0071850	Msa0609300	0.835724	1.420640e-56	-8.615850e-47
Msa0071860	Msa0609300	0.826972	2.022721e-54	-8.615850e-47
Msa0072520	Msa0609300	0.804205	2.415484e-49	-8.615850e-47
Msa0108880	Msa0609300	0.875541	2.841104e-68	-8.615850e-47
Msa0125210	Msa0609300	0.822899	1.850640e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609300	MtrunA17_Chr4g0041811	96.847	222	5	2	1	220	1	222	2.87e-158	436
Msa0609300	MtrunA17_Chr2g0319611	50.410	244	72	7	24	218	18	261	1.32e-65	203
Msa0609300	MtrunA17_Chr2g0324721	42.188	192	95	4	29	220	24	199	4.20e-41	139
Msa0609300	MtrunA17_Chr8g0360371	45.238	126	67	1	15	140	7	130	5.76e-37	131
Msa0609300	MtrunA17_Chr2g0317231	47.967	123	56	3	18	135	7	126	3.16e-34	129
Msa0609300	MtrunA17_Chr2g0324711	49.123	114	56	1	20	133	13	124	8.57e-34	124
Msa0609300	MtrunA17_Chr1g0150131	41.549	142	69	3	28	168	21	149	1.73e-33	119
Msa0609300	MtrunA17_Chr3g0136871	41.135	141	82	1	29	168	24	164	4.69e-33	117
Msa0609300	MtrunA17_Chr4g0040291	45.333	150	70	4	29	170	22	167	2.70e-31	113
Msa0609300	MtrunA17_Chr8g0385021	43.443	122	68	1	20	141	12	132	8.92e-28	106

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0609300	AT3G20570.1	47.368	190	90	2	11	200	9	188	6.98e-53	169
Msa0609300	AT4G28365.1	43.137	153	72	3	27	179	24	161	8.32e-35	123
Msa0609300	AT2G25060.1	47.899	119	61	1	18	135	17	135	8.66e-35	122
Msa0609300	AT4G30590.1	46.774	124	65	1	32	154	28	151	5.66e-34	120
Msa0609300	AT4G31840.1	37.654	162	81	3	17	176	11	154	7.31e-33	117
Msa0609300	AT5G25090.1	41.860	129	74	1	8	135	2	130	2.25e-32	116
Msa0609300	AT2G23990.1	39.011	182	97	2	35	215	31	199	5.20e-29	108
Msa0609300	AT5G57920.1	40.741	108	63	1	27	133	18	125	1.11e-28	106
Msa0609300	AT5G53870.2	38.690	168	96	4	29	191	27	192	2.09e-28	110
Msa0609300	AT5G57920.3	40.741	108	63	1	27	133	50	157	3.14e-28	106

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Find 63 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCAAACACCACCTCCTA+CGG	0.530029	4_2:-56663550	Msa0609300:CDS
AAAGGATTGTTGGAATCACT+TGG	0.491885	4_2:+56664055	None:intergenic
AAATGTTAAAGCTAGAGTGA+TGG	0.601239	4_2:+56664244	None:intergenic
AAATTGTGCAAACCGTGGAA+AGG	0.606922	4_2:+56664301	None:intergenic
AAGGTAGCCATTGAAATATG+AGG	0.588319	4_2:+56664193	None:intergenic
AAGTTCCAACAGAGCCAGCT+AGG	0.556887	4_2:+56663472	None:intergenic
AATTAGTCCATGCCTTGAGC+TGG	0.511550	4_2:-56664153	Msa0609300:CDS
ACCAGCACCTTCTAACCAAG+AGG	0.647755	4_2:-56663595	Msa0609300:CDS
AGTAGCATTGCTTGTTTGGT+TGG	0.452665	4_2:+56663647	None:intergenic
CATGAGACAAAACCAGCTCA+AGG	0.630389	4_2:+56664141	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATGTTAAAGCTAGAGTGA+TGG	+	chr4_2:56663269-56663288	None:intergenic	30.0%
TTGTGATCCTCATATTTCAA+TGG	-	chr4_2:56663310-56663329	Msa0609300:three_prime_UTR	30.0%
GATTAAAGAAGAACTTACCT+AGG	+	chr4_2:56663523-56663542	None:intergenic	30.0%
TCTTTAAACTCAACCAATCA+GGG	-	chr4_2:56663740-56663759	Msa0609300:CDS	30.0%
TTCTGTATGAACTAAGTCTT+TGG	-	chr4_2:56664161-56664180	Msa0609300:CDS	30.0%
AAATGTTAAAGCTAGAGTGA+TGG	+	chr4_2:56663269-56663288	None:intergenic	30.0%
TTGTGATCCTCATATTTCAA+TGG	-	chr4_2:56663310-56663329	Msa0609300:three_prime_UTR	30.0%
GATTAAAGAAGAACTTACCT+AGG	+	chr4_2:56663523-56663542	None:intergenic	30.0%
TCTTTAAACTCAACCAATCA+GGG	-	chr4_2:56663740-56663759	Msa0609300:CDS	30.0%
TTCTGTATGAACTAAGTCTT+TGG	-	chr4_2:56664161-56664180	Msa0609300:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	56663195	56664337	56663195	ID=Msa0609300;Name=Msa0609300
chr4_2	mRNA	56663195	56664337	56663195	ID=Msa0609300-mRNA-1;Parent=Msa0609300;Name=Msa0609300-mRNA-1;_AED=0.00;_eAED=0.00;_QI=134\|1\|1\|1\|1\|1\|2\|239\|220
chr4_2	exon	56663195	56663900	56663195	ID=Msa0609300-mRNA-1:exon:14627;Parent=Msa0609300-mRNA-1
chr4_2	exon	56664008	56664337	56664008	ID=Msa0609300-mRNA-1:exon:14626;Parent=Msa0609300-mRNA-1
chr4_2	five_prime_UTR	56664204	56664337	56664204	ID=Msa0609300-mRNA-1:five_prime_utr;Parent=Msa0609300-mRNA-1
chr4_2	CDS	56664008	56664203	56664008	ID=Msa0609300-mRNA-1:cds;Parent=Msa0609300-mRNA-1
chr4_2	CDS	56663434	56663900	56663434	ID=Msa0609300-mRNA-1:cds;Parent=Msa0609300-mRNA-1
chr4_2	three_prime_UTR	56663195	56663433	56663195	ID=Msa0609300-mRNA-1:three_prime_utr;Parent=Msa0609300-mRNA-1

Gene Sequence

>Msa0609300

ATGGCTACCTTCATTTTAAGGTCTAATAAATTAGTCCATGCCTTGAGCTGGTTTTGTCTCATGCTAATTGTTCATAAGAGTGCTGCTTATGATTTCATAGTTGGTGGCCAAAAGGGTTGGAGTGTTCCAAGTGATTCCAACAATCCTTTTAATCAATGGGCAGAAAAGAGCCGTTTTCAAGTTGGAGACTCCCTAGTGTTCAACTACCAATCTGGCAAAGACTCTGTGCTTTATGTAAAGAGTGAAGATTATGCAAGTTGCAACACTGGTTCACCTGTTGCAAAATTCTCTGATGGTCACACAGTCTTTAAACTCAACCAATCAGGGCCTCATTTCTTCATCAGTGGAAATAAAGACAACTGTCTCAAAAATGAGAAGGTAACAGTGATTGTTCTGTCTGACAGAAGCAACAATAATTCATCAAACACCAACCAAACAAGCAATGCTACTCCACCATCACCACAATCATCACCTCCTTCACCAGCACCTTCTAACCAAGAGGGTCAATCTCCACCTCCAGACACAAACCAAACACCACCTCCTACGGCTACTAGTGATCATGATCATCCTCCTCGTAATGGTGCTGCTTCAAACTTTGTCAGCCTAGCTGGCTCTGTTGGAACTTTCATGGCTTCAGCTCTAATTTTATCCAAGTATCTATGA

Protein sequence

>Msa0609300

MATFILRSNKLVHALSWFCLMLIVHKSAAYDFIVGGQKGWSVPSDSNNPFNQWAEKSRFQVGDSLVFNYQSGKDSVLYVKSEDYASCNTGSPVAKFSDGHTVFKLNQSGPHFFISGNKDNCLKNEKVTVIVLSDRSNNNSSNTNQTSNATPPSPQSSPPSPAPSNQEGQSPPPDTNQTPPPTATSDHDHPPRNGAASNFVSLAGSVGTFMASALILSKYL*