AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1323040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323040 MtrunA17_Chr8g0355821 94.156 154 9 0 1 154 1 154 3.08e-107 302
Msa1323040 MtrunA17_Chr8g0355811 89.103 156 17 0 1 156 1 156 5.85e-103 291
Msa1323040 MtrunA17_Chr8g0355801 84.615 156 24 0 1 156 1 156 2.99e-96 274
Msa1323040 MtrunA17_Chr8g0355791 82.051 156 28 0 1 156 1 156 1.44e-92 265
Msa1323040 MtrunA17_Chr8g0355771 80.769 156 30 0 1 156 1 156 2.09e-91 262
Msa1323040 MtrunA17_Chr8g0355741 73.885 157 40 1 1 156 1 157 1.13e-83 243
Msa1323040 MtrunA17_Chr8g0355721 70.701 157 44 2 1 156 1 156 9.38e-80 233
Msa1323040 MtrunA17_Chr8g0355661 70.064 157 45 2 1 156 1 156 2.23e-78 229
Msa1323040 MtrunA17_Chr8g0355781 67.763 152 48 1 1 151 1 152 8.18e-76 223
Msa1323040 MtrunA17_Chr8g0355761 67.105 152 49 1 1 151 1 152 2.67e-74 219
Msa1323040 MtrunA17_Chr8g0355641 64.744 156 54 1 1 155 1 156 8.43e-74 218
Msa1323040 MtrunA17_Chr1g0160371 41.830 153 85 3 1 150 1 152 1.55e-38 128
Msa1323040 MtrunA17_Chr1g0160381 38.562 153 90 3 1 150 5 156 9.42e-37 124
Msa1323040 MtrunA17_Chr1g0160391 37.821 156 88 5 1 148 1 155 1.10e-27 101
Msa1323040 MtrunA17_Chr8g0339261 33.333 150 96 4 6 153 8 155 1.94e-24 92.4
Msa1323040 MtrunA17_Chr1g0160801 37.162 148 83 6 7 151 7 147 4.31e-21 83.6
Msa1323040 MtrunA17_Chr1g0160351 39.785 93 53 2 61 150 1 93 2.32e-19 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1323040 AT5G28010.1 35.948 153 94 4 3 152 15 166 5.42e-25 94.7
Msa1323040 AT5G28010.2 35.948 153 94 4 3 152 15 166 5.42e-25 94.7
Msa1323040 AT1G70890.1 37.255 153 92 4 3 152 7 158 6.83e-24 91.7
Msa1323040 AT1G70840.1 35.948 153 94 4 3 152 20 171 2.35e-23 90.5
Msa1323040 AT1G70830.5 37.179 156 88 5 3 152 22 173 3.87e-23 90.1
Msa1323040 AT1G70830.3 36.943 157 89 5 3 153 22 174 5.19e-23 90.5
Msa1323040 AT1G70830.2 36.943 157 89 5 3 153 22 174 2.85e-22 89.7
Msa1323040 AT1G70830.1 36.943 157 89 5 3 153 22 174 5.58e-22 90.1
Msa1323040 AT1G70830.1 37.179 156 88 5 3 152 184 335 1.38e-21 89.4
Msa1323040 AT1G70830.4 37.179 156 88 5 3 152 137 288 5.69e-22 89.4
Msa1323040 AT1G70830.4 36.082 97 60 2 59 153 31 127 4.43e-12 62.8
Msa1323040 AT5G28000.1 33.333 153 98 4 3 152 13 164 8.59e-22 86.3
Msa1323040 AT1G70850.2 35.065 154 90 5 3 150 9 158 7.29e-21 85.5
Msa1323040 AT1G70850.3 35.294 153 89 5 3 149 9 157 1.68e-20 85.9
Msa1323040 AT1G70850.3 36.301 146 83 5 13 152 175 316 7.45e-20 84.3
Msa1323040 AT1G70850.1 35.294 153 89 5 3 149 9 157 1.68e-20 85.9
Msa1323040 AT1G70850.1 36.301 146 83 5 13 152 175 316 7.45e-20 84.3
Msa1323040 AT1G70870.1 43.750 96 52 2 56 150 44 138 7.51e-19 78.2
Msa1323040 AT1G14930.1 28.302 159 106 5 1 155 1 155 3.60e-18 76.6
Msa1323040 AT4G23670.1 31.875 160 91 6 1 151 1 151 6.71e-18 75.9
Msa1323040 AT2G01520.1 28.387 155 103 5 1 151 1 151 3.90e-17 73.9
Msa1323040 AT1G24020.1 30.921 152 102 3 1 150 1 151 5.88e-17 73.6
Msa1323040 AT1G24020.2 30.921 152 102 3 1 150 1 151 5.88e-17 73.6
Msa1323040 AT4G14060.1 29.032 155 102 5 1 151 1 151 2.08e-16 72.0
Msa1323040 AT2G01530.1 29.677 155 101 5 1 151 1 151 2.29e-16 72.0
Msa1323040 AT1G23120.1 27.660 141 96 4 10 149 10 145 3.32e-15 68.9
Msa1323040 AT1G70880.1 29.412 153 104 4 3 152 8 159 3.90e-15 68.9
Msa1323040 AT1G23120.2 27.660 141 96 4 10 149 60 195 9.01e-15 68.9
Msa1323040 AT1G14940.1 27.044 159 108 5 1 155 1 155 9.91e-15 67.8
Msa1323040 AT4G23680.1 27.922 154 103 5 1 150 1 150 1.32e-14 67.4
Msa1323040 AT1G14950.1 26.415 159 109 5 1 155 1 155 5.97e-14 65.9
Msa1323040 AT1G30990.1 27.152 151 105 5 1 149 1 148 1.95e-13 64.3
Msa1323040 AT3G26460.1 28.387 155 104 4 1 151 1 152 1.10e-12 62.4
Msa1323040 AT1G14960.1 24.516 155 113 4 1 153 1 153 1.61e-11 59.3

Find 43 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATGACCATCAATATCTT+TGG 0.335693 8_4:-30776115 None:intergenic
TTATGTGTGATGCAAATAAA+TGG 0.338325 8_4:+30776309 Msa1323040:three_prime_UTR
AGACATATTAATATTAAATA+AGG 0.347375 8_4:+30776172 Msa1323040:three_prime_UTR
AAGATCACTTTCAAGCTGTT+TGG 0.356858 8_4:+30775937 Msa1323040:CDS
ATGGAAATTCTGCTGTTAGA+TGG 0.411619 8_4:+30776019 Msa1323040:CDS
ATAAGGACGTATGGTCCAAC+GGG 0.419042 8_4:+30776189 Msa1323040:three_prime_UTR
TGTTTAAATTCATAGTTTGT+TGG 0.452494 8_4:+30776242 Msa1323040:three_prime_UTR
AGGTAGTCCATCCAGCCATT+TGG 0.456227 8_4:-30776081 None:intergenic
ATCACTTTCAAGCTGTTTGG+TGG 0.460728 8_4:+30775940 Msa1323040:CDS
AAAGGAACCAAAGATATTGA+TGG 0.467555 8_4:+30776108 Msa1323040:CDS
TAAACATTGGACTTATGTCA+TGG 0.493433 8_4:+30774534 Msa1323040:CDS
GGTAAACTTATCACTGAACT+TGG 0.495374 8_4:+30774382 Msa1323040:CDS
AGATTGGCATCACACTGATT+CGG 0.509801 8_4:+30774510 Msa1323040:CDS
TATCTTTGGTTCCTTTGCTA+AGG 0.518303 8_4:-30776101 None:intergenic
AGCTTCATGAAGGAGAAGAT+TGG 0.532439 8_4:+30774494 Msa1323040:CDS
TAATATTAAATAAGGACGTA+TGG 0.537245 8_4:+30776180 Msa1323040:three_prime_UTR
GACGTATGGTCCAACGGGGT+TGG 0.537616 8_4:+30776194 Msa1323040:three_prime_UTR
TGCACAAAGGCCAACCCCGT+TGG 0.553792 8_4:-30776204 None:intergenic
TAACTATAGCGTTTCTCTAA+CGG 0.554731 8_4:+30776366 Msa1323040:three_prime_UTR
ACACTGATTCGGTTAAACAT+TGG 0.558454 8_4:+30774521 Msa1323040:CDS
AATAAGGACGTATGGTCCAA+CGG 0.567686 8_4:+30776188 Msa1323040:three_prime_UTR
GAAGTGCTTAACAAGGCTGA+TGG 0.573383 8_4:+30776000 Msa1323040:CDS
CATCATACTAAGCTTCATGA+AGG 0.577072 8_4:+30774484 Msa1323040:CDS
ATGTTGATACTCCAAATGGC+TGG 0.578150 8_4:+30776070 Msa1323040:CDS
ATTGATGGTCATCTTGTCAA+GGG 0.586933 8_4:+30776123 Msa1323040:CDS
TGATACTCCAAATGGCTGGA+TGG 0.587093 8_4:+30776074 Msa1323040:CDS
AAACATTGGACTTATGTCAT+GGG 0.587276 8_4:+30774535 Msa1323040:CDS
TATTGATGGTCATCTTGTCA+AGG 0.589389 8_4:+30776122 Msa1323040:CDS
GAGGATGTTGATACTCCAAA+TGG 0.616840 8_4:+30776066 Msa1323040:CDS
GTAAACTTATCACTGAACTT+GGG 0.617151 8_4:+30774383 Msa1323040:CDS
TGGTACACACATGCAATGAG+AGG 0.624271 8_4:+30775887 Msa1323040:CDS
TATCATAACAACAGATGGTG+TGG 0.633773 8_4:+30775867 Msa1323040:intron
CATCTTAGAAGTGCTTAACA+AGG 0.644958 8_4:+30775993 Msa1323040:CDS
TTGATGGTCATCTTGTCAAG+GGG 0.646397 8_4:+30776124 Msa1323040:CDS
AAACTATGAATTTAAACACA+TGG 0.646555 8_4:-30776237 None:intergenic
ATGTTTCTATTCAATAAATG+TGG 0.646664 8_4:+30776269 Msa1323040:three_prime_UTR
TGGATGGACTACCTTAGCAA+AGG 0.650698 8_4:+30776090 Msa1323040:CDS
GAACTTGGGATCAAAACACC+CGG 0.651017 8_4:+30774397 Msa1323040:CDS
TGATGGTCATCTTGTCAAGG+GGG 0.696400 8_4:+30776125 Msa1323040:CDS
TGGTGTAATTGTGTGCACAA+AGG 0.730343 8_4:-30776217 None:intergenic
TGATTATGAGAAGAACAGTG+AGG 0.735998 8_4:+30776047 Msa1323040:CDS
GGTACACACATGCAATGAGA+GGG 0.748427 8_4:+30775888 Msa1323040:CDS
TAAGGACGTATGGTCCAACG+GGG 0.822650 8_4:+30776190 Msa1323040:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTAATAAACGTATTA+TGG - chr8_4:30774903-30774922 None:intergenic 10.0%
!! TTAAAGTAATATTGAAAATA+AGG - chr8_4:30775810-30775829 None:intergenic 10.0%
!!! AAAATGTTTTTTATTGTTTT+TGG + chr8_4:30775831-30775850 Msa1323040:intron 10.0%
!! AGACATATTAATATTAAATA+AGG + chr8_4:30776172-30776191 Msa1323040:three_prime_UTR 10.0%
!! ATTTATTAATAAACGTATTA+TGG - chr8_4:30774903-30774922 None:intergenic 10.0%
!! TTAAAGTAATATTGAAAATA+AGG - chr8_4:30775810-30775829 None:intergenic 10.0%
!!! AAAATGTTTTTTATTGTTTT+TGG + chr8_4:30775831-30775850 Msa1323040:intron 10.0%
!! AGACATATTAATATTAAATA+AGG + chr8_4:30776172-30776191 Msa1323040:three_prime_UTR 10.0%
!!! AAGAAAAATTATACTTTTCA+AGG + chr8_4:30774694-30774713 Msa1323040:intron 15.0%
!! ATTATAAATGAAGTCAATAT+AGG - chr8_4:30774818-30774837 None:intergenic 15.0%
!! AATGTTGAATATTTGAAAAT+GGG + chr8_4:30774950-30774969 Msa1323040:intron 15.0%
!! GGGTAAAATTAAATAAAAAA+AGG + chr8_4:30774970-30774989 Msa1323040:intron 15.0%
!!! TATAAAAGTCAAACTTTTAT+CGG + chr8_4:30775460-30775479 Msa1323040:intron 15.0%
!! ACTAATTAAGGATTTAAAAA+AGG + chr8_4:30775655-30775674 Msa1323040:intron 15.0%
!!! TAAAATAATGTTTCTACTTT+TGG + chr8_4:30775746-30775765 Msa1323040:intron 15.0%
!!! AAGAAAAATTATACTTTTCA+AGG + chr8_4:30774694-30774713 Msa1323040:intron 15.0%
!! ATTATAAATGAAGTCAATAT+AGG - chr8_4:30774818-30774837 None:intergenic 15.0%
!! AATGTTGAATATTTGAAAAT+GGG + chr8_4:30774950-30774969 Msa1323040:intron 15.0%
!! GGGTAAAATTAAATAAAAAA+AGG + chr8_4:30774970-30774989 Msa1323040:intron 15.0%
!!! TATAAAAGTCAAACTTTTAT+CGG + chr8_4:30775460-30775479 Msa1323040:intron 15.0%
!! ACTAATTAAGGATTTAAAAA+AGG + chr8_4:30775655-30775674 Msa1323040:intron 15.0%
!!! TAAAATAATGTTTCTACTTT+TGG + chr8_4:30775746-30775765 Msa1323040:intron 15.0%
!! AGAGAAGAAAGAAAAAATAA+GGG - chr8_4:30774317-30774336 None:intergenic 20.0%
!! AAGAGAAGAAAGAAAAAATA+AGG - chr8_4:30774318-30774337 None:intergenic 20.0%
!! TATAAGTTTCTTTCCAAAAA+TGG + chr8_4:30774351-30774370 Msa1323040:exon 20.0%
!! GAATGTTGAATATTTGAAAA+TGG + chr8_4:30774949-30774968 Msa1323040:intron 20.0%
!! ATTTATCTTTCTAGATCTAA+CGG + chr8_4:30775121-30775140 Msa1323040:intron 20.0%
!! ATCAAAAATCAAATATCACA+AGG - chr8_4:30775431-30775450 None:intergenic 20.0%
!! ATTTCCCTTGTAAAAAAAAA+AGG - chr8_4:30775552-30775571 None:intergenic 20.0%
!! ATCATAAAAACCTAATGATA+AGG - chr8_4:30775582-30775601 None:intergenic 20.0%
!!! TTAGGTTTTTATGATTTCTT+TGG + chr8_4:30775587-30775606 Msa1323040:intron 20.0%
!!! TGGTGAAACTAATATTTTTA+AGG + chr8_4:30775607-30775626 Msa1323040:intron 20.0%
!!! GTTTCTTAACAAATGTTTTA+AGG + chr8_4:30775768-30775787 Msa1323040:intron 20.0%
!! TAATATTAAATAAGGACGTA+TGG + chr8_4:30776180-30776199 Msa1323040:three_prime_UTR 20.0%
!! AAACTATGAATTTAAACACA+TGG - chr8_4:30776240-30776259 None:intergenic 20.0%
!!! TGTTTAAATTCATAGTTTGT+TGG + chr8_4:30776242-30776261 Msa1323040:three_prime_UTR 20.0%
!!! ATGTTTCTATTCAATAAATG+TGG + chr8_4:30776269-30776288 Msa1323040:three_prime_UTR 20.0%
!! AGAGAAGAAAGAAAAAATAA+GGG - chr8_4:30774317-30774336 None:intergenic 20.0%
!! AAGAGAAGAAAGAAAAAATA+AGG - chr8_4:30774318-30774337 None:intergenic 20.0%
!! TATAAGTTTCTTTCCAAAAA+TGG + chr8_4:30774351-30774370 Msa1323040:exon 20.0%
!! GAATGTTGAATATTTGAAAA+TGG + chr8_4:30774949-30774968 Msa1323040:intron 20.0%
!! ATTTATCTTTCTAGATCTAA+CGG + chr8_4:30775121-30775140 Msa1323040:intron 20.0%
!! ATCAAAAATCAAATATCACA+AGG - chr8_4:30775431-30775450 None:intergenic 20.0%
!! ATTTCCCTTGTAAAAAAAAA+AGG - chr8_4:30775552-30775571 None:intergenic 20.0%
!! ATCATAAAAACCTAATGATA+AGG - chr8_4:30775582-30775601 None:intergenic 20.0%
!!! TTAGGTTTTTATGATTTCTT+TGG + chr8_4:30775587-30775606 Msa1323040:intron 20.0%
!!! TGGTGAAACTAATATTTTTA+AGG + chr8_4:30775607-30775626 Msa1323040:intron 20.0%
!!! GTTTCTTAACAAATGTTTTA+AGG + chr8_4:30775768-30775787 Msa1323040:intron 20.0%
!! TAATATTAAATAAGGACGTA+TGG + chr8_4:30776180-30776199 Msa1323040:three_prime_UTR 20.0%
!! AAACTATGAATTTAAACACA+TGG - chr8_4:30776240-30776259 None:intergenic 20.0%
!!! TGTTTAAATTCATAGTTTGT+TGG + chr8_4:30776242-30776261 Msa1323040:three_prime_UTR 20.0%
!!! ATGTTTCTATTCAATAAATG+TGG + chr8_4:30776269-30776288 Msa1323040:three_prime_UTR 20.0%
! TTCTTAGACAAATTGTAGTA+CGG - chr8_4:30774679-30774698 None:intergenic 25.0%
!!! TACTTTTCAAGGCAATAATA+TGG + chr8_4:30774705-30774724 Msa1323040:intron 25.0%
! ATTGCCTTTATAAGTTCTTT+AGG - chr8_4:30774732-30774751 None:intergenic 25.0%
! ATTACATACAACTCATATAG+TGG - chr8_4:30775064-30775083 None:intergenic 25.0%
!!! TATTTGATTTTTGATCCTTG+TGG + chr8_4:30775436-30775455 Msa1323040:intron 25.0%
!!! TGCATCCTTTTTTTTTTACA+AGG + chr8_4:30775544-30775563 Msa1323040:intron 25.0%
!!! GCATCCTTTTTTTTTTACAA+GGG + chr8_4:30775545-30775564 Msa1323040:intron 25.0%
! AATAATGCATCCTTATCATT+AGG + chr8_4:30775569-30775588 Msa1323040:intron 25.0%
! TTATGTTAACAAGTGTCTTT+AGG + chr8_4:30775630-30775649 Msa1323040:intron 25.0%
! TATGTTAACAAGTGTCTTTA+GGG + chr8_4:30775631-30775650 Msa1323040:intron 25.0%
! TTATGTGTGATGCAAATAAA+TGG + chr8_4:30776309-30776328 Msa1323040:three_prime_UTR 25.0%
! TTCTTAGACAAATTGTAGTA+CGG - chr8_4:30774679-30774698 None:intergenic 25.0%
!!! TACTTTTCAAGGCAATAATA+TGG + chr8_4:30774705-30774724 Msa1323040:intron 25.0%
! ATTGCCTTTATAAGTTCTTT+AGG - chr8_4:30774732-30774751 None:intergenic 25.0%
! ATTACATACAACTCATATAG+TGG - chr8_4:30775064-30775083 None:intergenic 25.0%
!!! TATTTGATTTTTGATCCTTG+TGG + chr8_4:30775436-30775455 Msa1323040:intron 25.0%
!!! TGCATCCTTTTTTTTTTACA+AGG + chr8_4:30775544-30775563 Msa1323040:intron 25.0%
!!! GCATCCTTTTTTTTTTACAA+GGG + chr8_4:30775545-30775564 Msa1323040:intron 25.0%
! AATAATGCATCCTTATCATT+AGG + chr8_4:30775569-30775588 Msa1323040:intron 25.0%
! TTATGTTAACAAGTGTCTTT+AGG + chr8_4:30775630-30775649 Msa1323040:intron 25.0%
! TATGTTAACAAGTGTCTTTA+GGG + chr8_4:30775631-30775650 Msa1323040:intron 25.0%
! TTATGTGTGATGCAAATAAA+TGG + chr8_4:30776309-30776328 Msa1323040:three_prime_UTR 25.0%
GTAAACTTATCACTGAACTT+GGG + chr8_4:30774383-30774402 Msa1323040:CDS 30.0%
! TAGTTTGAAGAACTTTTCAC+CGG - chr8_4:30774419-30774438 None:intergenic 30.0%
TAAACATTGGACTTATGTCA+TGG + chr8_4:30774534-30774553 Msa1323040:CDS 30.0%
AAACATTGGACTTATGTCAT+GGG + chr8_4:30774535-30774554 Msa1323040:CDS 30.0%
AATCAAACAAGATGATGACT+TGG - chr8_4:30774592-30774611 None:intergenic 30.0%
TGGACCTAAAGAACTTATAA+AGG + chr8_4:30774725-30774744 Msa1323040:intron 30.0%
CATTGTATGGATTTATGCTT+AGG + chr8_4:30775029-30775048 Msa1323040:intron 30.0%
!! GTTGTATGTAATAGTTCTAG+TGG + chr8_4:30775072-30775091 Msa1323040:intron 30.0%
!! AATTTTTCGTTTTAGCGCAA+TGG + chr8_4:30775230-30775249 Msa1323040:intron 30.0%
GCAATGGATAAGTAATTACA+TGG + chr8_4:30775246-30775265 Msa1323040:intron 30.0%
! TGATTTTAAACTATCCTGTG+AGG + chr8_4:30775310-30775329 Msa1323040:intron 30.0%
! AGCGAAACTTGTTCAATTTA+CGG - chr8_4:30775349-30775368 None:intergenic 30.0%
!! GTTTGACTTTTATAACCACA+AGG - chr8_4:30775454-30775473 None:intergenic 30.0%
AAAGGAACCAAAGATATTGA+TGG + chr8_4:30776108-30776127 Msa1323040:CDS 30.0%
AAGATGACCATCAATATCTT+TGG - chr8_4:30776118-30776137 None:intergenic 30.0%
TAACTATAGCGTTTCTCTAA+CGG + chr8_4:30776366-30776385 Msa1323040:three_prime_UTR 30.0%
GTAAACTTATCACTGAACTT+GGG + chr8_4:30774383-30774402 Msa1323040:CDS 30.0%
! TAGTTTGAAGAACTTTTCAC+CGG - chr8_4:30774419-30774438 None:intergenic 30.0%
TAAACATTGGACTTATGTCA+TGG + chr8_4:30774534-30774553 Msa1323040:CDS 30.0%
AAACATTGGACTTATGTCAT+GGG + chr8_4:30774535-30774554 Msa1323040:CDS 30.0%
AATCAAACAAGATGATGACT+TGG - chr8_4:30774592-30774611 None:intergenic 30.0%
TGGACCTAAAGAACTTATAA+AGG + chr8_4:30774725-30774744 Msa1323040:intron 30.0%
CATTGTATGGATTTATGCTT+AGG + chr8_4:30775029-30775048 Msa1323040:intron 30.0%
!! GTTGTATGTAATAGTTCTAG+TGG + chr8_4:30775072-30775091 Msa1323040:intron 30.0%
!! AATTTTTCGTTTTAGCGCAA+TGG + chr8_4:30775230-30775249 Msa1323040:intron 30.0%
GCAATGGATAAGTAATTACA+TGG + chr8_4:30775246-30775265 Msa1323040:intron 30.0%
! TGATTTTAAACTATCCTGTG+AGG + chr8_4:30775310-30775329 Msa1323040:intron 30.0%
! AGCGAAACTTGTTCAATTTA+CGG - chr8_4:30775349-30775368 None:intergenic 30.0%
!! GTTTGACTTTTATAACCACA+AGG - chr8_4:30775454-30775473 None:intergenic 30.0%
AAAGGAACCAAAGATATTGA+TGG + chr8_4:30776108-30776127 Msa1323040:CDS 30.0%
AAGATGACCATCAATATCTT+TGG - chr8_4:30776118-30776137 None:intergenic 30.0%
TAACTATAGCGTTTCTCTAA+CGG + chr8_4:30776366-30776385 Msa1323040:three_prime_UTR 30.0%
GGTAAACTTATCACTGAACT+TGG + chr8_4:30774382-30774401 Msa1323040:CDS 35.0%
! AGTTTGAAGAACTTTTCACC+GGG - chr8_4:30774418-30774437 None:intergenic 35.0%
CATCATACTAAGCTTCATGA+AGG + chr8_4:30774484-30774503 Msa1323040:CDS 35.0%
!! ACACTGATTCGGTTAAACAT+TGG + chr8_4:30774521-30774540 Msa1323040:CDS 35.0%
!! TGCTACTTTAGTGCATTGTA+TGG + chr8_4:30775016-30775035 Msa1323040:intron 35.0%
! AATTCAAGTTCGAGTTCAAG+TGG + chr8_4:30775181-30775200 Msa1323040:intron 35.0%
TAATTACATGGCACGTATCT+AGG + chr8_4:30775258-30775277 Msa1323040:intron 35.0%
! GTTTAACTTTGTAACCTCAC+AGG - chr8_4:30775327-30775346 None:intergenic 35.0%
AACAAGTTTCGCTATCTTGT+CGG + chr8_4:30775356-30775375 Msa1323040:intron 35.0%
!! TGTCTTTAGGGCACTAATTA+AGG + chr8_4:30775643-30775662 Msa1323040:intron 35.0%
TCCTCTATCATAACAACAGA+TGG + chr8_4:30775862-30775881 Msa1323040:intron 35.0%
ACCATCTGTTGTTATGATAG+AGG - chr8_4:30775866-30775885 None:intergenic 35.0%
TATCATAACAACAGATGGTG+TGG + chr8_4:30775867-30775886 Msa1323040:intron 35.0%
AAGATCACTTTCAAGCTGTT+TGG + chr8_4:30775937-30775956 Msa1323040:CDS 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG + chr8_4:30775993-30776012 Msa1323040:CDS 35.0%
ATGGAAATTCTGCTGTTAGA+TGG + chr8_4:30776019-30776038 Msa1323040:CDS 35.0%
TGATTATGAGAAGAACAGTG+AGG + chr8_4:30776047-30776066 Msa1323040:CDS 35.0%
! TATCTTTGGTTCCTTTGCTA+AGG - chr8_4:30776104-30776123 None:intergenic 35.0%
! TATTGATGGTCATCTTGTCA+AGG + chr8_4:30776122-30776141 Msa1323040:CDS 35.0%
! ATTGATGGTCATCTTGTCAA+GGG + chr8_4:30776123-30776142 Msa1323040:CDS 35.0%
GGTAAACTTATCACTGAACT+TGG + chr8_4:30774382-30774401 Msa1323040:CDS 35.0%
! AGTTTGAAGAACTTTTCACC+GGG - chr8_4:30774418-30774437 None:intergenic 35.0%
CATCATACTAAGCTTCATGA+AGG + chr8_4:30774484-30774503 Msa1323040:CDS 35.0%
!! ACACTGATTCGGTTAAACAT+TGG + chr8_4:30774521-30774540 Msa1323040:CDS 35.0%
!! TGCTACTTTAGTGCATTGTA+TGG + chr8_4:30775016-30775035 Msa1323040:intron 35.0%
! AATTCAAGTTCGAGTTCAAG+TGG + chr8_4:30775181-30775200 Msa1323040:intron 35.0%
TAATTACATGGCACGTATCT+AGG + chr8_4:30775258-30775277 Msa1323040:intron 35.0%
! GTTTAACTTTGTAACCTCAC+AGG - chr8_4:30775327-30775346 None:intergenic 35.0%
AACAAGTTTCGCTATCTTGT+CGG + chr8_4:30775356-30775375 Msa1323040:intron 35.0%
!! TGTCTTTAGGGCACTAATTA+AGG + chr8_4:30775643-30775662 Msa1323040:intron 35.0%
TCCTCTATCATAACAACAGA+TGG + chr8_4:30775862-30775881 Msa1323040:intron 35.0%
ACCATCTGTTGTTATGATAG+AGG - chr8_4:30775866-30775885 None:intergenic 35.0%
TATCATAACAACAGATGGTG+TGG + chr8_4:30775867-30775886 Msa1323040:intron 35.0%
AAGATCACTTTCAAGCTGTT+TGG + chr8_4:30775937-30775956 Msa1323040:CDS 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG + chr8_4:30775993-30776012 Msa1323040:CDS 35.0%
ATGGAAATTCTGCTGTTAGA+TGG + chr8_4:30776019-30776038 Msa1323040:CDS 35.0%
TGATTATGAGAAGAACAGTG+AGG + chr8_4:30776047-30776066 Msa1323040:CDS 35.0%
! TATCTTTGGTTCCTTTGCTA+AGG - chr8_4:30776104-30776123 None:intergenic 35.0%
! TATTGATGGTCATCTTGTCA+AGG + chr8_4:30776122-30776141 Msa1323040:CDS 35.0%
! ATTGATGGTCATCTTGTCAA+GGG + chr8_4:30776123-30776142 Msa1323040:CDS 35.0%
TTTCCAAAAATGGTACTCGC+AGG + chr8_4:30774361-30774380 Msa1323040:exon 40.0%
! TTACCTGCGAGTACCATTTT+TGG - chr8_4:30774367-30774386 None:intergenic 40.0%
AGCTTCATGAAGGAGAAGAT+TGG + chr8_4:30774494-30774513 Msa1323040:CDS 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8_4:30774510-30774529 Msa1323040:CDS 40.0%
GAGTTCAAGTGGATGATTGA+GGG + chr8_4:30775192-30775211 Msa1323040:intron 40.0%
AGTTCAAGTGGATGATTGAG+GGG + chr8_4:30775193-30775212 Msa1323040:intron 40.0%
CCATAAAGCACAACATCTCT+TGG - chr8_4:30775392-30775411 None:intergenic 40.0%
CCAAGAGATGTTGTGCTTTA+TGG + chr8_4:30775389-30775408 Msa1323040:intron 40.0%
ATCACTTTCAAGCTGTTTGG+TGG + chr8_4:30775940-30775959 Msa1323040:CDS 40.0%
GAGGATGTTGATACTCCAAA+TGG + chr8_4:30776066-30776085 Msa1323040:CDS 40.0%
ATGTTGATACTCCAAATGGC+TGG + chr8_4:30776070-30776089 Msa1323040:CDS 40.0%
! TTGATGGTCATCTTGTCAAG+GGG + chr8_4:30776124-30776143 Msa1323040:CDS 40.0%
AATAAGGACGTATGGTCCAA+CGG + chr8_4:30776188-30776207 Msa1323040:three_prime_UTR 40.0%
TGGTGTAATTGTGTGCACAA+AGG - chr8_4:30776220-30776239 None:intergenic 40.0%
TTTCCAAAAATGGTACTCGC+AGG + chr8_4:30774361-30774380 Msa1323040:exon 40.0%
! TTACCTGCGAGTACCATTTT+TGG - chr8_4:30774367-30774386 None:intergenic 40.0%
AGCTTCATGAAGGAGAAGAT+TGG + chr8_4:30774494-30774513 Msa1323040:CDS 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8_4:30774510-30774529 Msa1323040:CDS 40.0%
GAGTTCAAGTGGATGATTGA+GGG + chr8_4:30775192-30775211 Msa1323040:intron 40.0%
AGTTCAAGTGGATGATTGAG+GGG + chr8_4:30775193-30775212 Msa1323040:intron 40.0%
CCATAAAGCACAACATCTCT+TGG - chr8_4:30775392-30775411 None:intergenic 40.0%
CCAAGAGATGTTGTGCTTTA+TGG + chr8_4:30775389-30775408 Msa1323040:intron 40.0%
ATCACTTTCAAGCTGTTTGG+TGG + chr8_4:30775940-30775959 Msa1323040:CDS 40.0%
GAGGATGTTGATACTCCAAA+TGG + chr8_4:30776066-30776085 Msa1323040:CDS 40.0%
ATGTTGATACTCCAAATGGC+TGG + chr8_4:30776070-30776089 Msa1323040:CDS 40.0%
! TTGATGGTCATCTTGTCAAG+GGG + chr8_4:30776124-30776143 Msa1323040:CDS 40.0%
AATAAGGACGTATGGTCCAA+CGG + chr8_4:30776188-30776207 Msa1323040:three_prime_UTR 40.0%
TGGTGTAATTGTGTGCACAA+AGG - chr8_4:30776220-30776239 None:intergenic 40.0%
GAACTTGGGATCAAAACACC+CGG + chr8_4:30774397-30774416 Msa1323040:CDS 45.0%
CGAGTTCAAGTGGATGATTG+AGG + chr8_4:30775191-30775210 Msa1323040:intron 45.0%
! ATTGAGGGGTAGTCTTGACT+CGG + chr8_4:30775207-30775226 Msa1323040:intron 45.0%
TGGTACACACATGCAATGAG+AGG + chr8_4:30775887-30775906 Msa1323040:CDS 45.0%
GGTACACACATGCAATGAGA+GGG + chr8_4:30775888-30775907 Msa1323040:CDS 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG + chr8_4:30776000-30776019 Msa1323040:CDS 45.0%
TGATACTCCAAATGGCTGGA+TGG + chr8_4:30776074-30776093 Msa1323040:CDS 45.0%
!! TGGATGGACTACCTTAGCAA+AGG + chr8_4:30776090-30776109 Msa1323040:CDS 45.0%
TGATGGTCATCTTGTCAAGG+GGG + chr8_4:30776125-30776144 Msa1323040:CDS 45.0%
TCATCTTGTCAAGGGGGAAA+AGG + chr8_4:30776131-30776150 Msa1323040:CDS 45.0%
ATAAGGACGTATGGTCCAAC+GGG + chr8_4:30776189-30776208 Msa1323040:three_prime_UTR 45.0%
GAACTTGGGATCAAAACACC+CGG + chr8_4:30774397-30774416 Msa1323040:CDS 45.0%
CGAGTTCAAGTGGATGATTG+AGG + chr8_4:30775191-30775210 Msa1323040:intron 45.0%
! ATTGAGGGGTAGTCTTGACT+CGG + chr8_4:30775207-30775226 Msa1323040:intron 45.0%
TGGTACACACATGCAATGAG+AGG + chr8_4:30775887-30775906 Msa1323040:CDS 45.0%
GGTACACACATGCAATGAGA+GGG + chr8_4:30775888-30775907 Msa1323040:CDS 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG + chr8_4:30776000-30776019 Msa1323040:CDS 45.0%
TGATACTCCAAATGGCTGGA+TGG + chr8_4:30776074-30776093 Msa1323040:CDS 45.0%
!! TGGATGGACTACCTTAGCAA+AGG + chr8_4:30776090-30776109 Msa1323040:CDS 45.0%
TGATGGTCATCTTGTCAAGG+GGG + chr8_4:30776125-30776144 Msa1323040:CDS 45.0%
TCATCTTGTCAAGGGGGAAA+AGG + chr8_4:30776131-30776150 Msa1323040:CDS 45.0%
ATAAGGACGTATGGTCCAAC+GGG + chr8_4:30776189-30776208 Msa1323040:three_prime_UTR 45.0%
CTATGCCGAGCAACTCATGT+GGG + chr8_4:30775156-30775175 Msa1323040:intron 50.0%
TATGCCGAGCAACTCATGTG+GGG + chr8_4:30775157-30775176 Msa1323040:intron 50.0%
ATTCCCCCACATGAGTTGCT+CGG - chr8_4:30775164-30775183 None:intergenic 50.0%
AGGTAGTCCATCCAGCCATT+TGG - chr8_4:30776084-30776103 None:intergenic 50.0%
TAAGGACGTATGGTCCAACG+GGG + chr8_4:30776190-30776209 Msa1323040:three_prime_UTR 50.0%
CTATGCCGAGCAACTCATGT+GGG + chr8_4:30775156-30775175 Msa1323040:intron 50.0%
TATGCCGAGCAACTCATGTG+GGG + chr8_4:30775157-30775176 Msa1323040:intron 50.0%
ATTCCCCCACATGAGTTGCT+CGG - chr8_4:30775164-30775183 None:intergenic 50.0%
AGGTAGTCCATCCAGCCATT+TGG - chr8_4:30776084-30776103 None:intergenic 50.0%
TAAGGACGTATGGTCCAACG+GGG + chr8_4:30776190-30776209 Msa1323040:three_prime_UTR 50.0%
TTGCTCGGCATAGCAGGTGT+GGG - chr8_4:30775149-30775168 None:intergenic 55.0%
CATGAGTTGCTCGGCATAGC+AGG - chr8_4:30775155-30775174 None:intergenic 55.0%
GCTATGCCGAGCAACTCATG+TGG + chr8_4:30775155-30775174 Msa1323040:intron 55.0%
ATGCCGAGCAACTCATGTGG+GGG + chr8_4:30775158-30775177 Msa1323040:intron 55.0%
TTGCTCGGCATAGCAGGTGT+GGG - chr8_4:30775149-30775168 None:intergenic 55.0%
CATGAGTTGCTCGGCATAGC+AGG - chr8_4:30775155-30775174 None:intergenic 55.0%
GCTATGCCGAGCAACTCATG+TGG + chr8_4:30775155-30775174 Msa1323040:intron 55.0%
ATGCCGAGCAACTCATGTGG+GGG + chr8_4:30775158-30775177 Msa1323040:intron 55.0%
GTTGCTCGGCATAGCAGGTG+TGG - chr8_4:30775150-30775169 None:intergenic 60.0%
GACGTATGGTCCAACGGGGT+TGG + chr8_4:30776194-30776213 Msa1323040:three_prime_UTR 60.0%
TGCACAAAGGCCAACCCCGT+TGG - chr8_4:30776207-30776226 None:intergenic 60.0%
GTTGCTCGGCATAGCAGGTG+TGG - chr8_4:30775150-30775169 None:intergenic 60.0%
GACGTATGGTCCAACGGGGT+TGG + chr8_4:30776194-30776213 Msa1323040:three_prime_UTR 60.0%
TGCACAAAGGCCAACCCCGT+TGG - chr8_4:30776207-30776226 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 30774280 30776433 30774280 ID=Msa1323040;Name=Msa1323040
chr8_4 mRNA 30774280 30776433 30774280 ID=Msa1323040-mRNA-1;Parent=Msa1323040;Name=Msa1323040-mRNA-1;_AED=0.01;_eAED=0.01;_QI=90|1|1|1|1|1|2|269|156
chr8_4 exon 30774280 30774556 30774280 ID=Msa1323040-mRNA-1:exon:10157;Parent=Msa1323040-mRNA-1
chr8_4 exon 30775881 30776433 30775881 ID=Msa1323040-mRNA-1:exon:10158;Parent=Msa1323040-mRNA-1
chr8_4 five_prime_UTR 30774280 30774369 30774280 ID=Msa1323040-mRNA-1:five_prime_utr;Parent=Msa1323040-mRNA-1
chr8_4 CDS 30774370 30774556 30774370 ID=Msa1323040-mRNA-1:cds;Parent=Msa1323040-mRNA-1
chr8_4 CDS 30775881 30776164 30775881 ID=Msa1323040-mRNA-1:cds;Parent=Msa1323040-mRNA-1
chr8_4 three_prime_UTR 30776165 30776433 30776165 ID=Msa1323040-mRNA-1:three_prime_utr;Parent=Msa1323040-mRNA-1
Gene Sequence

>Msa1323040

ATGGTACTCGCAGGTAAACTTATCACTGAACTTGGGATCAAAACACCCGGTGAAAAGTTCTTCAAACTATTTGCAACTCAACTTCACGAAGTGCAAAACGTTGTTGAAAGATGTCATCATACTAAGCTTCATGAAGGAGAAGATTGGCATCACACTGATTCGGTTAAACATTGGACTTATGTCATGGATGGTGTGGTACACACATGCAATGAGAGGGTTGAAGAAGTTGATGAGCAGAACAAAAAGATCACTTTCAAGCTGTTTGGTGGAGATATTGAAAACTATAAAGTCTTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAATTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAGTGAGGATGTTGATACTCCAAATGGCTGGATGGACTACCTTAGCAAAGGAACCAAAGATATTGATGGTCATCTTGTCAAGGGGGAAAAGGTAGCTCTATAA
Protein sequence

>Msa1323040

MVLAGKLITELGIKTPGEKFFKLFATQLHEVQNVVERCHHTKLHEGEDWHHTDSVKHWTYVMDGVVHTCNERVEEVDEQNKKITFKLFGGDIENYKVFKLILEVLNKADGNSAVRWTIDYEKNSEDVDTPNGWMDYLSKGTKDIDGHLVKGEKVAL*