AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1173280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1173280 MtrunA17_Chr8g0355821 94.156 154 9 0 1 154 1 154 3.08e-107 302
Msa1173280 MtrunA17_Chr8g0355811 89.103 156 17 0 1 156 1 156 5.85e-103 291
Msa1173280 MtrunA17_Chr8g0355801 84.615 156 24 0 1 156 1 156 2.99e-96 274
Msa1173280 MtrunA17_Chr8g0355791 82.051 156 28 0 1 156 1 156 1.44e-92 265
Msa1173280 MtrunA17_Chr8g0355771 80.769 156 30 0 1 156 1 156 2.09e-91 262
Msa1173280 MtrunA17_Chr8g0355741 73.885 157 40 1 1 156 1 157 1.13e-83 243
Msa1173280 MtrunA17_Chr8g0355721 70.701 157 44 2 1 156 1 156 9.38e-80 233
Msa1173280 MtrunA17_Chr8g0355661 70.064 157 45 2 1 156 1 156 2.23e-78 229
Msa1173280 MtrunA17_Chr8g0355781 67.763 152 48 1 1 151 1 152 8.18e-76 223
Msa1173280 MtrunA17_Chr8g0355761 67.105 152 49 1 1 151 1 152 2.67e-74 219
Msa1173280 MtrunA17_Chr8g0355641 64.744 156 54 1 1 155 1 156 8.43e-74 218
Msa1173280 MtrunA17_Chr1g0160371 41.830 153 85 3 1 150 1 152 1.55e-38 128
Msa1173280 MtrunA17_Chr1g0160381 38.562 153 90 3 1 150 5 156 9.42e-37 124
Msa1173280 MtrunA17_Chr1g0160391 37.821 156 88 5 1 148 1 155 1.10e-27 101
Msa1173280 MtrunA17_Chr8g0339261 33.333 150 96 4 6 153 8 155 1.94e-24 92.4
Msa1173280 MtrunA17_Chr1g0160801 37.162 148 83 6 7 151 7 147 4.31e-21 83.6
Msa1173280 MtrunA17_Chr1g0160351 39.785 93 53 2 61 150 1 93 2.32e-19 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1173280 AT5G28010.1 35.948 153 94 4 3 152 15 166 5.42e-25 94.7
Msa1173280 AT5G28010.2 35.948 153 94 4 3 152 15 166 5.42e-25 94.7
Msa1173280 AT1G70890.1 37.255 153 92 4 3 152 7 158 6.83e-24 91.7
Msa1173280 AT1G70840.1 35.948 153 94 4 3 152 20 171 2.35e-23 90.5
Msa1173280 AT1G70830.5 37.179 156 88 5 3 152 22 173 3.87e-23 90.1
Msa1173280 AT1G70830.3 36.943 157 89 5 3 153 22 174 5.19e-23 90.5
Msa1173280 AT1G70830.2 36.943 157 89 5 3 153 22 174 2.85e-22 89.7
Msa1173280 AT1G70830.1 36.943 157 89 5 3 153 22 174 5.58e-22 90.1
Msa1173280 AT1G70830.1 37.179 156 88 5 3 152 184 335 1.38e-21 89.4
Msa1173280 AT1G70830.4 37.179 156 88 5 3 152 137 288 5.69e-22 89.4
Msa1173280 AT1G70830.4 36.082 97 60 2 59 153 31 127 4.43e-12 62.8
Msa1173280 AT5G28000.1 33.333 153 98 4 3 152 13 164 8.59e-22 86.3
Msa1173280 AT1G70850.2 35.065 154 90 5 3 150 9 158 7.29e-21 85.5
Msa1173280 AT1G70850.3 35.294 153 89 5 3 149 9 157 1.68e-20 85.9
Msa1173280 AT1G70850.3 36.301 146 83 5 13 152 175 316 7.45e-20 84.3
Msa1173280 AT1G70850.1 35.294 153 89 5 3 149 9 157 1.68e-20 85.9
Msa1173280 AT1G70850.1 36.301 146 83 5 13 152 175 316 7.45e-20 84.3
Msa1173280 AT1G70870.1 43.750 96 52 2 56 150 44 138 7.51e-19 78.2
Msa1173280 AT1G14930.1 28.302 159 106 5 1 155 1 155 3.60e-18 76.6
Msa1173280 AT4G23670.1 31.875 160 91 6 1 151 1 151 6.71e-18 75.9
Msa1173280 AT2G01520.1 28.387 155 103 5 1 151 1 151 3.90e-17 73.9
Msa1173280 AT1G24020.1 30.921 152 102 3 1 150 1 151 5.88e-17 73.6
Msa1173280 AT1G24020.2 30.921 152 102 3 1 150 1 151 5.88e-17 73.6
Msa1173280 AT4G14060.1 29.032 155 102 5 1 151 1 151 2.08e-16 72.0
Msa1173280 AT2G01530.1 29.677 155 101 5 1 151 1 151 2.29e-16 72.0
Msa1173280 AT1G23120.1 27.660 141 96 4 10 149 10 145 3.32e-15 68.9
Msa1173280 AT1G70880.1 29.412 153 104 4 3 152 8 159 3.90e-15 68.9
Msa1173280 AT1G23120.2 27.660 141 96 4 10 149 60 195 9.01e-15 68.9
Msa1173280 AT1G14940.1 27.044 159 108 5 1 155 1 155 9.91e-15 67.8
Msa1173280 AT4G23680.1 27.922 154 103 5 1 150 1 150 1.32e-14 67.4
Msa1173280 AT1G14950.1 26.415 159 109 5 1 155 1 155 5.97e-14 65.9
Msa1173280 AT1G30990.1 27.152 151 105 5 1 149 1 148 1.95e-13 64.3
Msa1173280 AT3G26460.1 28.387 155 104 4 1 151 1 152 1.10e-12 62.4
Msa1173280 AT1G14960.1 24.516 155 113 4 1 153 1 153 1.61e-11 59.3

Find 43 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGATGACCATCAATATCTT+TGG 0.335693 8_1:+3634694 None:intergenic
TTATGTGTGATGCAAATAAA+TGG 0.338325 8_1:-3634500 Msa1173280:three_prime_UTR
AGACATATTAATATTAAATA+AGG 0.347375 8_1:-3634637 Msa1173280:three_prime_UTR
AAGATCACTTTCAAGCTGTT+TGG 0.356858 8_1:-3634872 Msa1173280:CDS
ATGGAAATTCTGCTGTTAGA+TGG 0.411619 8_1:-3634790 Msa1173280:CDS
ATAAGGACGTATGGTCCAAC+GGG 0.419042 8_1:-3634620 Msa1173280:three_prime_UTR
TGTTTAAATTCATAGTTTGT+TGG 0.452494 8_1:-3634567 Msa1173280:three_prime_UTR
AGGTAGTCCATCCAGCCATT+TGG 0.456227 8_1:+3634728 None:intergenic
ATCACTTTCAAGCTGTTTGG+TGG 0.460728 8_1:-3634869 Msa1173280:CDS
AAAGGAACCAAAGATATTGA+TGG 0.467555 8_1:-3634701 Msa1173280:CDS
TAAACATTGGACTTATGTCA+TGG 0.493433 8_1:-3636275 Msa1173280:CDS
GGTAAACTTATCACTGAACT+TGG 0.495374 8_1:-3636427 Msa1173280:CDS
AGATTGGCATCACACTGATT+CGG 0.509801 8_1:-3636299 Msa1173280:CDS
TATCTTTGGTTCCTTTGCTA+AGG 0.518303 8_1:+3634708 None:intergenic
AGCTTCATGAAGGAGAAGAT+TGG 0.532439 8_1:-3636315 Msa1173280:CDS
TAATATTAAATAAGGACGTA+TGG 0.537245 8_1:-3634629 Msa1173280:three_prime_UTR
GACGTATGGTCCAACGGGGT+TGG 0.537616 8_1:-3634615 Msa1173280:three_prime_UTR
TGCACAAAGGCCAACCCCGT+TGG 0.553792 8_1:+3634605 None:intergenic
TAACTATAGCGTTTCTCTAA+CGG 0.554731 8_1:-3634443 Msa1173280:three_prime_UTR
ACACTGATTCGGTTAAACAT+TGG 0.558454 8_1:-3636288 Msa1173280:CDS
AATAAGGACGTATGGTCCAA+CGG 0.567686 8_1:-3634621 Msa1173280:three_prime_UTR
GAAGTGCTTAACAAGGCTGA+TGG 0.573383 8_1:-3634809 Msa1173280:CDS
CATCATACTAAGCTTCATGA+AGG 0.577072 8_1:-3636325 Msa1173280:CDS
ATGTTGATACTCCAAATGGC+TGG 0.578150 8_1:-3634739 Msa1173280:CDS
ATTGATGGTCATCTTGTCAA+GGG 0.586933 8_1:-3634686 Msa1173280:CDS
TGATACTCCAAATGGCTGGA+TGG 0.587093 8_1:-3634735 Msa1173280:CDS
AAACATTGGACTTATGTCAT+GGG 0.587276 8_1:-3636274 Msa1173280:intron
TATTGATGGTCATCTTGTCA+AGG 0.589389 8_1:-3634687 Msa1173280:CDS
GAGGATGTTGATACTCCAAA+TGG 0.616840 8_1:-3634743 Msa1173280:CDS
GTAAACTTATCACTGAACTT+GGG 0.617151 8_1:-3636426 Msa1173280:CDS
TGGTACACACATGCAATGAG+AGG 0.624271 8_1:-3634922 Msa1173280:CDS
TATCATAACAACAGATGGTG+TGG 0.633773 8_1:-3634942 Msa1173280:intron
CATCTTAGAAGTGCTTAACA+AGG 0.644958 8_1:-3634816 Msa1173280:CDS
TTGATGGTCATCTTGTCAAG+GGG 0.646397 8_1:-3634685 Msa1173280:CDS
AAACTATGAATTTAAACACA+TGG 0.646555 8_1:+3634572 None:intergenic
ATGTTTCTATTCAATAAATG+TGG 0.646664 8_1:-3634540 Msa1173280:three_prime_UTR
TGGATGGACTACCTTAGCAA+AGG 0.650698 8_1:-3634719 Msa1173280:CDS
GAACTTGGGATCAAAACACC+CGG 0.651017 8_1:-3636412 Msa1173280:CDS
TGATGGTCATCTTGTCAAGG+GGG 0.696400 8_1:-3634684 Msa1173280:CDS
TGGTGTAATTGTGTGCACAA+AGG 0.730343 8_1:+3634592 None:intergenic
TGATTATGAGAAGAACAGTG+AGG 0.735998 8_1:-3634762 Msa1173280:CDS
GGTACACACATGCAATGAGA+GGG 0.748427 8_1:-3634921 Msa1173280:CDS
TAAGGACGTATGGTCCAACG+GGG 0.822650 8_1:-3634619 Msa1173280:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTAATAAACGTATTA+TGG + chr8_1:3635021-3635040 None:intergenic 10.0%
!! TTAAAGTAATATTGAAAATA+AGG + chr8_1:3635928-3635947 None:intergenic 10.0%
!!! AAAATGTTTTTTATTGTTTT+TGG - chr8_1:3635949-3635968 Msa1173280:intron 10.0%
!! AGACATATTAATATTAAATA+AGG - chr8_1:3636290-3636309 Msa1173280:CDS 10.0%
!! ATTTATTAATAAACGTATTA+TGG + chr8_1:3635021-3635040 None:intergenic 10.0%
!! TTAAAGTAATATTGAAAATA+AGG + chr8_1:3635928-3635947 None:intergenic 10.0%
!!! AAAATGTTTTTTATTGTTTT+TGG - chr8_1:3635949-3635968 Msa1173280:intron 10.0%
!! AGACATATTAATATTAAATA+AGG - chr8_1:3636290-3636309 Msa1173280:CDS 10.0%
!!! AAGAAAAATTATACTTTTCA+AGG - chr8_1:3634812-3634831 Msa1173280:CDS 15.0%
!! ATTATAAATGAAGTCAATAT+AGG + chr8_1:3634936-3634955 None:intergenic 15.0%
!! AATGTTGAATATTTGAAAAT+GGG - chr8_1:3635068-3635087 Msa1173280:intron 15.0%
!! GGGTAAAATTAAATAAAAAA+AGG - chr8_1:3635088-3635107 Msa1173280:intron 15.0%
!!! TATAAAAGTCAAACTTTTAT+CGG - chr8_1:3635578-3635597 Msa1173280:intron 15.0%
!! ACTAATTAAGGATTTAAAAA+AGG - chr8_1:3635773-3635792 Msa1173280:intron 15.0%
!!! TAAAATAATGTTTCTACTTT+TGG - chr8_1:3635864-3635883 Msa1173280:intron 15.0%
!!! AAGAAAAATTATACTTTTCA+AGG - chr8_1:3634812-3634831 Msa1173280:CDS 15.0%
!! ATTATAAATGAAGTCAATAT+AGG + chr8_1:3634936-3634955 None:intergenic 15.0%
!! AATGTTGAATATTTGAAAAT+GGG - chr8_1:3635068-3635087 Msa1173280:intron 15.0%
!! GGGTAAAATTAAATAAAAAA+AGG - chr8_1:3635088-3635107 Msa1173280:intron 15.0%
!!! TATAAAAGTCAAACTTTTAT+CGG - chr8_1:3635578-3635597 Msa1173280:intron 15.0%
!! ACTAATTAAGGATTTAAAAA+AGG - chr8_1:3635773-3635792 Msa1173280:intron 15.0%
!!! TAAAATAATGTTTCTACTTT+TGG - chr8_1:3635864-3635883 Msa1173280:intron 15.0%
!! AGAGAAGAAAGAAAAAATAA+GGG + chr8_1:3634435-3634454 None:intergenic 20.0%
!! AAGAGAAGAAAGAAAAAATA+AGG + chr8_1:3634436-3634455 None:intergenic 20.0%
!! TATAAGTTTCTTTCCAAAAA+TGG - chr8_1:3634469-3634488 Msa1173280:three_prime_UTR 20.0%
!! GAATGTTGAATATTTGAAAA+TGG - chr8_1:3635067-3635086 Msa1173280:intron 20.0%
!! ATTTATCTTTCTAGATCTAA+CGG - chr8_1:3635239-3635258 Msa1173280:intron 20.0%
!! ATCAAAAATCAAATATCACA+AGG + chr8_1:3635549-3635568 None:intergenic 20.0%
!! ATTTCCCTTGTAAAAAAAAA+AGG + chr8_1:3635670-3635689 None:intergenic 20.0%
!! ATCATAAAAACCTAATGATA+AGG + chr8_1:3635700-3635719 None:intergenic 20.0%
!!! TTAGGTTTTTATGATTTCTT+TGG - chr8_1:3635705-3635724 Msa1173280:intron 20.0%
!!! TGGTGAAACTAATATTTTTA+AGG - chr8_1:3635725-3635744 Msa1173280:intron 20.0%
!!! GTTTCTTAACAAATGTTTTA+AGG - chr8_1:3635886-3635905 Msa1173280:intron 20.0%
!! TAATATTAAATAAGGACGTA+TGG - chr8_1:3636298-3636317 Msa1173280:CDS 20.0%
!! AAACTATGAATTTAAACACA+TGG + chr8_1:3636358-3636377 None:intergenic 20.0%
!!! TGTTTAAATTCATAGTTTGT+TGG - chr8_1:3636360-3636379 Msa1173280:CDS 20.0%
!!! ATGTTTCTATTCAATAAATG+TGG - chr8_1:3636387-3636406 Msa1173280:CDS 20.0%
!! AGAGAAGAAAGAAAAAATAA+GGG + chr8_1:3634435-3634454 None:intergenic 20.0%
!! AAGAGAAGAAAGAAAAAATA+AGG + chr8_1:3634436-3634455 None:intergenic 20.0%
!! TATAAGTTTCTTTCCAAAAA+TGG - chr8_1:3634469-3634488 Msa1173280:three_prime_UTR 20.0%
!! GAATGTTGAATATTTGAAAA+TGG - chr8_1:3635067-3635086 Msa1173280:intron 20.0%
!! ATTTATCTTTCTAGATCTAA+CGG - chr8_1:3635239-3635258 Msa1173280:intron 20.0%
!! ATCAAAAATCAAATATCACA+AGG + chr8_1:3635549-3635568 None:intergenic 20.0%
!! ATTTCCCTTGTAAAAAAAAA+AGG + chr8_1:3635670-3635689 None:intergenic 20.0%
!! ATCATAAAAACCTAATGATA+AGG + chr8_1:3635700-3635719 None:intergenic 20.0%
!!! TTAGGTTTTTATGATTTCTT+TGG - chr8_1:3635705-3635724 Msa1173280:intron 20.0%
!!! TGGTGAAACTAATATTTTTA+AGG - chr8_1:3635725-3635744 Msa1173280:intron 20.0%
!!! GTTTCTTAACAAATGTTTTA+AGG - chr8_1:3635886-3635905 Msa1173280:intron 20.0%
!! TAATATTAAATAAGGACGTA+TGG - chr8_1:3636298-3636317 Msa1173280:CDS 20.0%
!! AAACTATGAATTTAAACACA+TGG + chr8_1:3636358-3636377 None:intergenic 20.0%
!!! TGTTTAAATTCATAGTTTGT+TGG - chr8_1:3636360-3636379 Msa1173280:CDS 20.0%
!!! ATGTTTCTATTCAATAAATG+TGG - chr8_1:3636387-3636406 Msa1173280:CDS 20.0%
! TTCTTAGACAAATTGTAGTA+CGG + chr8_1:3634797-3634816 None:intergenic 25.0%
!!! TACTTTTCAAGGCAATAATA+TGG - chr8_1:3634823-3634842 Msa1173280:CDS 25.0%
! ATTGCCTTTATAAGTTCTTT+AGG + chr8_1:3634850-3634869 None:intergenic 25.0%
! ATTACATACAACTCATATAG+TGG + chr8_1:3635182-3635201 None:intergenic 25.0%
!!! TATTTGATTTTTGATCCTTG+TGG - chr8_1:3635554-3635573 Msa1173280:intron 25.0%
!!! TGCATCCTTTTTTTTTTACA+AGG - chr8_1:3635662-3635681 Msa1173280:intron 25.0%
!!! GCATCCTTTTTTTTTTACAA+GGG - chr8_1:3635663-3635682 Msa1173280:intron 25.0%
! AATAATGCATCCTTATCATT+AGG - chr8_1:3635687-3635706 Msa1173280:intron 25.0%
! TTATGTTAACAAGTGTCTTT+AGG - chr8_1:3635748-3635767 Msa1173280:intron 25.0%
! TATGTTAACAAGTGTCTTTA+GGG - chr8_1:3635749-3635768 Msa1173280:intron 25.0%
! TTATGTGTGATGCAAATAAA+TGG - chr8_1:3636427-3636446 Msa1173280:CDS 25.0%
! TTCTTAGACAAATTGTAGTA+CGG + chr8_1:3634797-3634816 None:intergenic 25.0%
!!! TACTTTTCAAGGCAATAATA+TGG - chr8_1:3634823-3634842 Msa1173280:CDS 25.0%
! ATTGCCTTTATAAGTTCTTT+AGG + chr8_1:3634850-3634869 None:intergenic 25.0%
! ATTACATACAACTCATATAG+TGG + chr8_1:3635182-3635201 None:intergenic 25.0%
!!! TATTTGATTTTTGATCCTTG+TGG - chr8_1:3635554-3635573 Msa1173280:intron 25.0%
!!! TGCATCCTTTTTTTTTTACA+AGG - chr8_1:3635662-3635681 Msa1173280:intron 25.0%
!!! GCATCCTTTTTTTTTTACAA+GGG - chr8_1:3635663-3635682 Msa1173280:intron 25.0%
! AATAATGCATCCTTATCATT+AGG - chr8_1:3635687-3635706 Msa1173280:intron 25.0%
! TTATGTTAACAAGTGTCTTT+AGG - chr8_1:3635748-3635767 Msa1173280:intron 25.0%
! TATGTTAACAAGTGTCTTTA+GGG - chr8_1:3635749-3635768 Msa1173280:intron 25.0%
! TTATGTGTGATGCAAATAAA+TGG - chr8_1:3636427-3636446 Msa1173280:CDS 25.0%
GTAAACTTATCACTGAACTT+GGG - chr8_1:3634501-3634520 Msa1173280:three_prime_UTR 30.0%
! TAGTTTGAAGAACTTTTCAC+CGG + chr8_1:3634537-3634556 None:intergenic 30.0%
TAAACATTGGACTTATGTCA+TGG - chr8_1:3634652-3634671 Msa1173280:exon 30.0%
AAACATTGGACTTATGTCAT+GGG - chr8_1:3634653-3634672 Msa1173280:exon 30.0%
AATCAAACAAGATGATGACT+TGG + chr8_1:3634710-3634729 None:intergenic 30.0%
TGGACCTAAAGAACTTATAA+AGG - chr8_1:3634843-3634862 Msa1173280:CDS 30.0%
CATTGTATGGATTTATGCTT+AGG - chr8_1:3635147-3635166 Msa1173280:intron 30.0%
!! GTTGTATGTAATAGTTCTAG+TGG - chr8_1:3635190-3635209 Msa1173280:intron 30.0%
!! AATTTTTCGTTTTAGCGCAA+TGG - chr8_1:3635348-3635367 Msa1173280:intron 30.0%
GCAATGGATAAGTAATTACA+TGG - chr8_1:3635364-3635383 Msa1173280:intron 30.0%
! TGATTTTAAACTATCCTGTG+AGG - chr8_1:3635428-3635447 Msa1173280:intron 30.0%
! AGCGAAACTTGTTCAATTTA+CGG + chr8_1:3635467-3635486 None:intergenic 30.0%
!! GTTTGACTTTTATAACCACA+AGG + chr8_1:3635572-3635591 None:intergenic 30.0%
AAAGGAACCAAAGATATTGA+TGG - chr8_1:3636226-3636245 Msa1173280:intron 30.0%
AAGATGACCATCAATATCTT+TGG + chr8_1:3636236-3636255 None:intergenic 30.0%
TAACTATAGCGTTTCTCTAA+CGG - chr8_1:3636484-3636503 Msa1173280:five_prime_UTR 30.0%
GTAAACTTATCACTGAACTT+GGG - chr8_1:3634501-3634520 Msa1173280:three_prime_UTR 30.0%
! TAGTTTGAAGAACTTTTCAC+CGG + chr8_1:3634537-3634556 None:intergenic 30.0%
TAAACATTGGACTTATGTCA+TGG - chr8_1:3634652-3634671 Msa1173280:exon 30.0%
AAACATTGGACTTATGTCAT+GGG - chr8_1:3634653-3634672 Msa1173280:exon 30.0%
AATCAAACAAGATGATGACT+TGG + chr8_1:3634710-3634729 None:intergenic 30.0%
TGGACCTAAAGAACTTATAA+AGG - chr8_1:3634843-3634862 Msa1173280:CDS 30.0%
CATTGTATGGATTTATGCTT+AGG - chr8_1:3635147-3635166 Msa1173280:intron 30.0%
!! GTTGTATGTAATAGTTCTAG+TGG - chr8_1:3635190-3635209 Msa1173280:intron 30.0%
!! AATTTTTCGTTTTAGCGCAA+TGG - chr8_1:3635348-3635367 Msa1173280:intron 30.0%
GCAATGGATAAGTAATTACA+TGG - chr8_1:3635364-3635383 Msa1173280:intron 30.0%
! TGATTTTAAACTATCCTGTG+AGG - chr8_1:3635428-3635447 Msa1173280:intron 30.0%
! AGCGAAACTTGTTCAATTTA+CGG + chr8_1:3635467-3635486 None:intergenic 30.0%
!! GTTTGACTTTTATAACCACA+AGG + chr8_1:3635572-3635591 None:intergenic 30.0%
AAAGGAACCAAAGATATTGA+TGG - chr8_1:3636226-3636245 Msa1173280:intron 30.0%
AAGATGACCATCAATATCTT+TGG + chr8_1:3636236-3636255 None:intergenic 30.0%
TAACTATAGCGTTTCTCTAA+CGG - chr8_1:3636484-3636503 Msa1173280:five_prime_UTR 30.0%
GGTAAACTTATCACTGAACT+TGG - chr8_1:3634500-3634519 Msa1173280:three_prime_UTR 35.0%
! AGTTTGAAGAACTTTTCACC+GGG + chr8_1:3634536-3634555 None:intergenic 35.0%
CATCATACTAAGCTTCATGA+AGG - chr8_1:3634602-3634621 Msa1173280:three_prime_UTR 35.0%
!! ACACTGATTCGGTTAAACAT+TGG - chr8_1:3634639-3634658 Msa1173280:three_prime_UTR 35.0%
!! TGCTACTTTAGTGCATTGTA+TGG - chr8_1:3635134-3635153 Msa1173280:intron 35.0%
! AATTCAAGTTCGAGTTCAAG+TGG - chr8_1:3635299-3635318 Msa1173280:intron 35.0%
TAATTACATGGCACGTATCT+AGG - chr8_1:3635376-3635395 Msa1173280:intron 35.0%
! GTTTAACTTTGTAACCTCAC+AGG + chr8_1:3635445-3635464 None:intergenic 35.0%
AACAAGTTTCGCTATCTTGT+CGG - chr8_1:3635474-3635493 Msa1173280:intron 35.0%
!! TGTCTTTAGGGCACTAATTA+AGG - chr8_1:3635761-3635780 Msa1173280:intron 35.0%
TCCTCTATCATAACAACAGA+TGG - chr8_1:3635980-3635999 Msa1173280:intron 35.0%
ACCATCTGTTGTTATGATAG+AGG + chr8_1:3635984-3636003 None:intergenic 35.0%
TATCATAACAACAGATGGTG+TGG - chr8_1:3635985-3636004 Msa1173280:intron 35.0%
AAGATCACTTTCAAGCTGTT+TGG - chr8_1:3636055-3636074 Msa1173280:intron 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG - chr8_1:3636111-3636130 Msa1173280:intron 35.0%
ATGGAAATTCTGCTGTTAGA+TGG - chr8_1:3636137-3636156 Msa1173280:intron 35.0%
TGATTATGAGAAGAACAGTG+AGG - chr8_1:3636165-3636184 Msa1173280:intron 35.0%
! TATCTTTGGTTCCTTTGCTA+AGG + chr8_1:3636222-3636241 None:intergenic 35.0%
! TATTGATGGTCATCTTGTCA+AGG - chr8_1:3636240-3636259 Msa1173280:intron 35.0%
! ATTGATGGTCATCTTGTCAA+GGG - chr8_1:3636241-3636260 Msa1173280:intron 35.0%
GGTAAACTTATCACTGAACT+TGG - chr8_1:3634500-3634519 Msa1173280:three_prime_UTR 35.0%
! AGTTTGAAGAACTTTTCACC+GGG + chr8_1:3634536-3634555 None:intergenic 35.0%
CATCATACTAAGCTTCATGA+AGG - chr8_1:3634602-3634621 Msa1173280:three_prime_UTR 35.0%
!! ACACTGATTCGGTTAAACAT+TGG - chr8_1:3634639-3634658 Msa1173280:three_prime_UTR 35.0%
!! TGCTACTTTAGTGCATTGTA+TGG - chr8_1:3635134-3635153 Msa1173280:intron 35.0%
! AATTCAAGTTCGAGTTCAAG+TGG - chr8_1:3635299-3635318 Msa1173280:intron 35.0%
TAATTACATGGCACGTATCT+AGG - chr8_1:3635376-3635395 Msa1173280:intron 35.0%
! GTTTAACTTTGTAACCTCAC+AGG + chr8_1:3635445-3635464 None:intergenic 35.0%
AACAAGTTTCGCTATCTTGT+CGG - chr8_1:3635474-3635493 Msa1173280:intron 35.0%
!! TGTCTTTAGGGCACTAATTA+AGG - chr8_1:3635761-3635780 Msa1173280:intron 35.0%
TCCTCTATCATAACAACAGA+TGG - chr8_1:3635980-3635999 Msa1173280:intron 35.0%
ACCATCTGTTGTTATGATAG+AGG + chr8_1:3635984-3636003 None:intergenic 35.0%
TATCATAACAACAGATGGTG+TGG - chr8_1:3635985-3636004 Msa1173280:intron 35.0%
AAGATCACTTTCAAGCTGTT+TGG - chr8_1:3636055-3636074 Msa1173280:intron 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG - chr8_1:3636111-3636130 Msa1173280:intron 35.0%
ATGGAAATTCTGCTGTTAGA+TGG - chr8_1:3636137-3636156 Msa1173280:intron 35.0%
TGATTATGAGAAGAACAGTG+AGG - chr8_1:3636165-3636184 Msa1173280:intron 35.0%
! TATCTTTGGTTCCTTTGCTA+AGG + chr8_1:3636222-3636241 None:intergenic 35.0%
! TATTGATGGTCATCTTGTCA+AGG - chr8_1:3636240-3636259 Msa1173280:intron 35.0%
! ATTGATGGTCATCTTGTCAA+GGG - chr8_1:3636241-3636260 Msa1173280:intron 35.0%
TTTCCAAAAATGGTACTCGC+AGG - chr8_1:3634479-3634498 Msa1173280:three_prime_UTR 40.0%
! TTACCTGCGAGTACCATTTT+TGG + chr8_1:3634485-3634504 None:intergenic 40.0%
AGCTTCATGAAGGAGAAGAT+TGG - chr8_1:3634612-3634631 Msa1173280:three_prime_UTR 40.0%
! AGATTGGCATCACACTGATT+CGG - chr8_1:3634628-3634647 Msa1173280:three_prime_UTR 40.0%
GAGTTCAAGTGGATGATTGA+GGG - chr8_1:3635310-3635329 Msa1173280:intron 40.0%
AGTTCAAGTGGATGATTGAG+GGG - chr8_1:3635311-3635330 Msa1173280:intron 40.0%
CCATAAAGCACAACATCTCT+TGG + chr8_1:3635510-3635529 None:intergenic 40.0%
CCAAGAGATGTTGTGCTTTA+TGG - chr8_1:3635507-3635526 Msa1173280:intron 40.0%
ATCACTTTCAAGCTGTTTGG+TGG - chr8_1:3636058-3636077 Msa1173280:intron 40.0%
GAGGATGTTGATACTCCAAA+TGG - chr8_1:3636184-3636203 Msa1173280:intron 40.0%
ATGTTGATACTCCAAATGGC+TGG - chr8_1:3636188-3636207 Msa1173280:intron 40.0%
! TTGATGGTCATCTTGTCAAG+GGG - chr8_1:3636242-3636261 Msa1173280:intron 40.0%
AATAAGGACGTATGGTCCAA+CGG - chr8_1:3636306-3636325 Msa1173280:CDS 40.0%
TGGTGTAATTGTGTGCACAA+AGG + chr8_1:3636338-3636357 None:intergenic 40.0%
TTTCCAAAAATGGTACTCGC+AGG - chr8_1:3634479-3634498 Msa1173280:three_prime_UTR 40.0%
! TTACCTGCGAGTACCATTTT+TGG + chr8_1:3634485-3634504 None:intergenic 40.0%
AGCTTCATGAAGGAGAAGAT+TGG - chr8_1:3634612-3634631 Msa1173280:three_prime_UTR 40.0%
! AGATTGGCATCACACTGATT+CGG - chr8_1:3634628-3634647 Msa1173280:three_prime_UTR 40.0%
GAGTTCAAGTGGATGATTGA+GGG - chr8_1:3635310-3635329 Msa1173280:intron 40.0%
AGTTCAAGTGGATGATTGAG+GGG - chr8_1:3635311-3635330 Msa1173280:intron 40.0%
CCATAAAGCACAACATCTCT+TGG + chr8_1:3635510-3635529 None:intergenic 40.0%
CCAAGAGATGTTGTGCTTTA+TGG - chr8_1:3635507-3635526 Msa1173280:intron 40.0%
ATCACTTTCAAGCTGTTTGG+TGG - chr8_1:3636058-3636077 Msa1173280:intron 40.0%
GAGGATGTTGATACTCCAAA+TGG - chr8_1:3636184-3636203 Msa1173280:intron 40.0%
ATGTTGATACTCCAAATGGC+TGG - chr8_1:3636188-3636207 Msa1173280:intron 40.0%
! TTGATGGTCATCTTGTCAAG+GGG - chr8_1:3636242-3636261 Msa1173280:intron 40.0%
AATAAGGACGTATGGTCCAA+CGG - chr8_1:3636306-3636325 Msa1173280:CDS 40.0%
TGGTGTAATTGTGTGCACAA+AGG + chr8_1:3636338-3636357 None:intergenic 40.0%
GAACTTGGGATCAAAACACC+CGG - chr8_1:3634515-3634534 Msa1173280:three_prime_UTR 45.0%
CGAGTTCAAGTGGATGATTG+AGG - chr8_1:3635309-3635328 Msa1173280:intron 45.0%
! ATTGAGGGGTAGTCTTGACT+CGG - chr8_1:3635325-3635344 Msa1173280:intron 45.0%
TGGTACACACATGCAATGAG+AGG - chr8_1:3636005-3636024 Msa1173280:intron 45.0%
GGTACACACATGCAATGAGA+GGG - chr8_1:3636006-3636025 Msa1173280:intron 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG - chr8_1:3636118-3636137 Msa1173280:intron 45.0%
TGATACTCCAAATGGCTGGA+TGG - chr8_1:3636192-3636211 Msa1173280:intron 45.0%
!! TGGATGGACTACCTTAGCAA+AGG - chr8_1:3636208-3636227 Msa1173280:intron 45.0%
TGATGGTCATCTTGTCAAGG+GGG - chr8_1:3636243-3636262 Msa1173280:intron 45.0%
TCATCTTGTCAAGGGGGAAA+AGG - chr8_1:3636249-3636268 Msa1173280:intron 45.0%
ATAAGGACGTATGGTCCAAC+GGG - chr8_1:3636307-3636326 Msa1173280:CDS 45.0%
GAACTTGGGATCAAAACACC+CGG - chr8_1:3634515-3634534 Msa1173280:three_prime_UTR 45.0%
CGAGTTCAAGTGGATGATTG+AGG - chr8_1:3635309-3635328 Msa1173280:intron 45.0%
! ATTGAGGGGTAGTCTTGACT+CGG - chr8_1:3635325-3635344 Msa1173280:intron 45.0%
TGGTACACACATGCAATGAG+AGG - chr8_1:3636005-3636024 Msa1173280:intron 45.0%
GGTACACACATGCAATGAGA+GGG - chr8_1:3636006-3636025 Msa1173280:intron 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG - chr8_1:3636118-3636137 Msa1173280:intron 45.0%
TGATACTCCAAATGGCTGGA+TGG - chr8_1:3636192-3636211 Msa1173280:intron 45.0%
!! TGGATGGACTACCTTAGCAA+AGG - chr8_1:3636208-3636227 Msa1173280:intron 45.0%
TGATGGTCATCTTGTCAAGG+GGG - chr8_1:3636243-3636262 Msa1173280:intron 45.0%
TCATCTTGTCAAGGGGGAAA+AGG - chr8_1:3636249-3636268 Msa1173280:intron 45.0%
ATAAGGACGTATGGTCCAAC+GGG - chr8_1:3636307-3636326 Msa1173280:CDS 45.0%
CTATGCCGAGCAACTCATGT+GGG - chr8_1:3635274-3635293 Msa1173280:intron 50.0%
TATGCCGAGCAACTCATGTG+GGG - chr8_1:3635275-3635294 Msa1173280:intron 50.0%
ATTCCCCCACATGAGTTGCT+CGG + chr8_1:3635282-3635301 None:intergenic 50.0%
AGGTAGTCCATCCAGCCATT+TGG + chr8_1:3636202-3636221 None:intergenic 50.0%
TAAGGACGTATGGTCCAACG+GGG - chr8_1:3636308-3636327 Msa1173280:CDS 50.0%
CTATGCCGAGCAACTCATGT+GGG - chr8_1:3635274-3635293 Msa1173280:intron 50.0%
TATGCCGAGCAACTCATGTG+GGG - chr8_1:3635275-3635294 Msa1173280:intron 50.0%
ATTCCCCCACATGAGTTGCT+CGG + chr8_1:3635282-3635301 None:intergenic 50.0%
AGGTAGTCCATCCAGCCATT+TGG + chr8_1:3636202-3636221 None:intergenic 50.0%
TAAGGACGTATGGTCCAACG+GGG - chr8_1:3636308-3636327 Msa1173280:CDS 50.0%
TTGCTCGGCATAGCAGGTGT+GGG + chr8_1:3635267-3635286 None:intergenic 55.0%
CATGAGTTGCTCGGCATAGC+AGG + chr8_1:3635273-3635292 None:intergenic 55.0%
GCTATGCCGAGCAACTCATG+TGG - chr8_1:3635273-3635292 Msa1173280:intron 55.0%
ATGCCGAGCAACTCATGTGG+GGG - chr8_1:3635276-3635295 Msa1173280:intron 55.0%
TTGCTCGGCATAGCAGGTGT+GGG + chr8_1:3635267-3635286 None:intergenic 55.0%
CATGAGTTGCTCGGCATAGC+AGG + chr8_1:3635273-3635292 None:intergenic 55.0%
GCTATGCCGAGCAACTCATG+TGG - chr8_1:3635273-3635292 Msa1173280:intron 55.0%
ATGCCGAGCAACTCATGTGG+GGG - chr8_1:3635276-3635295 Msa1173280:intron 55.0%
GTTGCTCGGCATAGCAGGTG+TGG + chr8_1:3635268-3635287 None:intergenic 60.0%
GACGTATGGTCCAACGGGGT+TGG - chr8_1:3636312-3636331 Msa1173280:CDS 60.0%
TGCACAAAGGCCAACCCCGT+TGG + chr8_1:3636325-3636344 None:intergenic 60.0%
GTTGCTCGGCATAGCAGGTG+TGG + chr8_1:3635268-3635287 None:intergenic 60.0%
GACGTATGGTCCAACGGGGT+TGG - chr8_1:3636312-3636331 Msa1173280:CDS 60.0%
TGCACAAAGGCCAACCCCGT+TGG + chr8_1:3636325-3636344 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 3634398 3636551 3634398 ID=Msa1173280;Name=Msa1173280
chr8_1 mRNA 3634398 3636551 3634398 ID=Msa1173280-mRNA-1;Parent=Msa1173280;Name=Msa1173280-mRNA-1;_AED=0.01;_eAED=0.01;_QI=90|1|1|1|1|1|2|269|156
chr8_1 exon 3634398 3634950 3634398 ID=Msa1173280-mRNA-1:exon:1276;Parent=Msa1173280-mRNA-1
chr8_1 exon 3636275 3636551 3636275 ID=Msa1173280-mRNA-1:exon:1275;Parent=Msa1173280-mRNA-1
chr8_1 five_prime_UTR 3636462 3636551 3636462 ID=Msa1173280-mRNA-1:five_prime_utr;Parent=Msa1173280-mRNA-1
chr8_1 CDS 3636275 3636461 3636275 ID=Msa1173280-mRNA-1:cds;Parent=Msa1173280-mRNA-1
chr8_1 CDS 3634667 3634950 3634667 ID=Msa1173280-mRNA-1:cds;Parent=Msa1173280-mRNA-1
chr8_1 three_prime_UTR 3634398 3634666 3634398 ID=Msa1173280-mRNA-1:three_prime_utr;Parent=Msa1173280-mRNA-1
Gene Sequence

>Msa1173280

ATGGTACTCGCAGGTAAACTTATCACTGAACTTGGGATCAAAACACCCGGTGAAAAGTTCTTCAAACTATTTGCAACTCAACTTCACGAAGTGCAAAACGTTGTTGAAAGATGTCATCATACTAAGCTTCATGAAGGAGAAGATTGGCATCACACTGATTCGGTTAAACATTGGACTTATGTCATGGATGGTGTGGTACACACATGCAATGAGAGGGTTGAAGAAGTTGATGAGCAGAACAAAAAGATCACTTTCAAGCTGTTTGGTGGAGATATTGAAAACTATAAAGTCTTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAATTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAGTGAGGATGTTGATACTCCAAATGGCTGGATGGACTACCTTAGCAAAGGAACCAAAGATATTGATGGTCATCTTGTCAAGGGGGAAAAGGTAGCTCTATAA
Protein sequence

>Msa1173280

MVLAGKLITELGIKTPGEKFFKLFATQLHEVQNVVERCHHTKLHEGEDWHHTDSVKHWTYVMDGVVHTCNERVEEVDEQNKKITFKLFGGDIENYKVFKLILEVLNKADGNSAVRWTIDYEKNSEDVDTPNGWMDYLSKGTKDIDGHLVKGEKVAL*