Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0307380 | XP_003630087.1 | 96.000 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 1.30e-44 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0307380 | G7LAG4 | 96.000 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 6.21e-45 | 147 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026780 | Msa0307380 | 0.830492 | 2.849456e-55 | -8.615850e-47 |
| Msa0044010 | Msa0307380 | 0.803885 | 2.815763e-49 | -8.615850e-47 |
| Msa0077320 | Msa0307380 | 0.819155 | 1.346912e-52 | -8.615850e-47 |
| Msa0091300 | Msa0307380 | -0.802403 | 5.703542e-49 | -8.615850e-47 |
| Msa0098240 | Msa0307380 | 0.811501 | 6.782980e-51 | -8.615850e-47 |
| Msa0150330 | Msa0307380 | 0.802157 | 6.408325e-49 | -8.615850e-47 |
| Msa0199620 | Msa0307380 | 0.809512 | 1.824637e-50 | -8.615850e-47 |
| Msa0220170 | Msa0307380 | 0.805914 | 1.060259e-49 | -8.615850e-47 |
| Msa0233920 | Msa0307380 | 0.811203 | 7.873369e-51 | -8.615850e-47 |
| Msa0261460 | Msa0307380 | 0.814450 | 1.531248e-51 | -8.615850e-47 |
| Msa0307380 | Msa0312640 | 0.830189 | 3.377417e-55 | -8.615850e-47 |
| Msa0307380 | Msa0355080 | -0.803913 | 2.778050e-49 | -8.615850e-47 |
| Msa0307380 | Msa0377820 | 0.818651 | 1.753583e-52 | -8.615850e-47 |
| Msa0307380 | Msa0384590 | 0.836946 | 6.945336e-57 | -8.615850e-47 |
| Msa0307380 | Msa0434660 | 0.819239 | 1.289090e-52 | -8.615850e-47 |
| Msa0307380 | Msa0472930 | 0.821939 | 3.091521e-53 | -8.615850e-47 |
| Msa0307380 | Msa0522540 | 0.836935 | 6.988585e-57 | -8.615850e-47 |
| Msa0307380 | Msa0526490 | -0.806692 | 7.272274e-50 | -8.615850e-47 |
| Msa0307380 | Msa0526500 | -0.836595 | 8.536626e-57 | -8.615850e-47 |
| Msa0307380 | Msa0563440 | 0.832049 | 1.179792e-55 | -8.615850e-47 |
| Msa0307380 | Msa0577690 | 0.878969 | 1.825453e-69 | -8.615850e-47 |
| Msa0307380 | Msa0587430 | 0.806700 | 7.241058e-50 | -8.615850e-47 |
| Msa0307380 | Msa0603090 | 0.820913 | 5.335778e-53 | -8.615850e-47 |
| Msa0307380 | Msa0626920 | 0.832687 | 8.200651e-56 | -8.615850e-47 |
| Msa0307380 | Msa0651870 | 0.881006 | 3.433511e-70 | -8.615850e-47 |
| Msa0307380 | Msa0651890 | 0.882946 | 6.796772e-71 | -8.615850e-47 |
| Msa0307380 | Msa0660150 | 0.800699 | 1.275209e-48 | -8.615850e-47 |
| Msa0307380 | Msa0700410 | 0.879078 | 1.671051e-69 | -8.615850e-47 |
| Msa0307380 | Msa0829580 | 0.805955 | 1.039663e-49 | -8.615850e-47 |
| Msa0307380 | Msa1026970 | 0.822283 | 2.574122e-53 | -8.615850e-47 |
| Msa0307380 | Msa1087890 | 0.811540 | 6.651322e-51 | -8.615850e-47 |
| Msa0307380 | Msa1111900 | 0.806345 | 8.607053e-50 | -8.615850e-47 |
| Msa0307380 | Msa1133760 | 0.830241 | 3.280347e-55 | -8.615850e-47 |
| Msa0307380 | Msa1197720 | 0.819709 | 1.007453e-52 | -8.615850e-47 |
| Msa0307380 | Msa1208530 | 0.813935 | 1.989445e-51 | -8.615850e-47 |
| Msa0307380 | Msa1251410 | 0.857203 | 1.915095e-62 | -8.615850e-47 |
| Msa0307380 | Msa1260220 | 0.802401 | 5.710384e-49 | -8.615850e-47 |
| Msa0307380 | Msa1307790 | 0.815053 | 1.125597e-51 | -8.615850e-47 |
| Msa0307380 | Msa1334750 | 0.813068 | 3.086711e-51 | -8.615850e-47 |
| Msa0307380 | Msa1358470 | 0.911625 | 4.919232e-83 | -8.615850e-47 |
| Msa0307380 | Msa1363280 | -0.848839 | 4.744515e-60 | -8.615850e-47 |
| Msa0307380 | Msa1369830 | 0.814999 | 1.157150e-51 | -8.615850e-47 |
| Msa0307380 | Msa1385970 | 0.800296 | 1.540467e-48 | -8.615850e-47 |
| Msa0307380 | Msa1403940 | 0.840513 | 8.312402e-58 | -8.615850e-47 |
| Msa0307380 | Msa1417110 | 0.803953 | 2.725303e-49 | -8.615850e-47 |
| Msa0307380 | Msa1422480 | 0.811098 | 8.298906e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0307380 | MtrunA17_Chr8g0382821 | 96.000 | 75 | 3 | 0 | 1 | 75 | 1 | 75 | 1.20e-48 | 147 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAATAGCAAATTGAGTTT+TGG | 0.254507 | 2_4:+312580 | None:intergenic |
| GCAACTTGTTCAGAGTGTTT+TGG | 0.336230 | 2_4:-312606 | Msa0307380:CDS |
| GTCTTCTTGTTATTCACTAC+TGG | 0.369369 | 2_4:-312654 | Msa0307380:CDS |
| CTTCTTCTCCTCCTTCAACT+TGG | 0.390702 | 2_4:+312628 | None:intergenic |
| ACTACTGGTTTCCAAGTTGA+AGG | 0.427104 | 2_4:-312639 | Msa0307380:CDS |
| CATTGCTATGTTAGTAGCTG+AGG | 0.547311 | 2_4:-312750 | Msa0307380:CDS |
| GTGGTGCTGCTGTAGAAACA+TGG | 0.583385 | 2_4:-312688 | Msa0307380:CDS |
| ACTGGTTTCCAAGTTGAAGG+AGG | 0.631072 | 2_4:-312636 | Msa0307380:CDS |
| TGGTGCTGCTGTAGAAACAT+GGG | 0.694208 | 2_4:-312687 | Msa0307380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTCAAGAGTTTAAAAAATGC+TGG | - | chr2_4:312598-312617 | Msa0307380:CDS | 30.0% | |
| AAGAGTTTAAAAAATGCTGG+TGG | - | chr2_4:312601-312620 | Msa0307380:CDS | 30.0% | |
| ! | GCAATAGCAAATTGAGTTTT+GGG | + | chr2_4:312733-312752 | None:intergenic | 30.0% |
| ! | AGCAATAGCAAATTGAGTTT+TGG | + | chr2_4:312734-312753 | None:intergenic | 30.0% |
| AGTTTAAAAAATGCTGGTGG+TGG | - | chr2_4:312604-312623 | Msa0307380:CDS | 35.0% | |
| !! | GTCTTCTTGTTATTCACTAC+TGG | - | chr2_4:312657-312676 | Msa0307380:CDS | 35.0% |
| CATTGCTATGTTAGTAGCTG+AGG | - | chr2_4:312561-312580 | Msa0307380:CDS | 40.0% | |
| !! | ACTACTGGTTTCCAAGTTGA+AGG | - | chr2_4:312672-312691 | Msa0307380:CDS | 40.0% |
| !! | GCAACTTGTTCAGAGTGTTT+TGG | - | chr2_4:312705-312724 | Msa0307380:CDS | 40.0% |
| !! | TGGTGCTGCTGTAGAAACAT+GGG | - | chr2_4:312624-312643 | Msa0307380:CDS | 45.0% |
| ! | ACTGGTTTCCAAGTTGAAGG+AGG | - | chr2_4:312675-312694 | Msa0307380:CDS | 45.0% |
| CTTCTTCTCCTCCTTCAACT+TGG | + | chr2_4:312686-312705 | None:intergenic | 45.0% | |
| !! | GTGGTGCTGCTGTAGAAACA+TGG | - | chr2_4:312623-312642 | Msa0307380:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 312553 | 312780 | 312553 | ID=Msa0307380;Name=Msa0307380 |
| chr2_4 | mRNA | 312553 | 312780 | 312553 | ID=Msa0307380-mRNA-1;Parent=Msa0307380;Name=Msa0307380-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|75 |
| chr2_4 | exon | 312553 | 312780 | 312553 | ID=Msa0307380-mRNA-1:exon:108;Parent=Msa0307380-mRNA-1 |
| chr2_4 | CDS | 312553 | 312780 | 312553 | ID=Msa0307380-mRNA-1:cds;Parent=Msa0307380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
