Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1358470 | XP_003630087.1 | 94.667 | 75 | 4 | 0 | 1 | 75 | 1 | 75 | 1.21e-43 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1358470 | G7LAG4 | 94.667 | 75 | 4 | 0 | 1 | 75 | 1 | 75 | 5.77e-44 | 145 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0077320 | Msa1358470 | 0.809438 | 1.892545e-50 | -8.615850e-47 |
| Msa1358470 | Msa1363280 | -0.811198 | 7.893269e-51 | -8.615850e-47 |
| Msa1358470 | Msa1403940 | 0.830931 | 2.223460e-55 | -8.615850e-47 |
| Msa0651870 | Msa1358470 | 0.823598 | 1.270706e-53 | -8.615850e-47 |
| Msa0651890 | Msa1358470 | 0.827034 | 1.954823e-54 | -8.615850e-47 |
| Msa0700410 | Msa1358470 | 0.846294 | 2.377145e-59 | -8.615850e-47 |
| Msa1251410 | Msa1358470 | 0.812227 | 4.713457e-51 | -8.615850e-47 |
| Msa0474720 | Msa1358470 | 0.806307 | 8.765774e-50 | -8.615850e-47 |
| Msa0307380 | Msa1358470 | 0.911625 | 4.919232e-83 | -8.615850e-47 |
| Msa0563440 | Msa1358470 | 0.804929 | 1.705805e-49 | -8.615850e-47 |
| Msa0577690 | Msa1358470 | 0.832834 | 7.537044e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1358470 | MtrunA17_Chr8g0382821 | 94.667 | 75 | 4 | 0 | 1 | 75 | 1 | 75 | 1.11e-47 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAATAGCAAATTGAGTTT+TGG | 0.254507 | tig0016338:+69044 | None:intergenic |
| GCAACTTGTTCAGAGTGTTT+TGG | 0.336230 | tig0016338:-69070 | Msa1358470:CDS |
| GTCTTCTTGTTATTCACTAC+TGG | 0.369369 | tig0016338:-69118 | Msa1358470:CDS |
| GTGGTGCTGCTGTAGAAATA+TGG | 0.372555 | tig0016338:-69152 | Msa1358470:CDS |
| CTTCTTCTCCTCCTTCAACT+TGG | 0.390702 | tig0016338:+69092 | None:intergenic |
| ACTACTGGTTTCCAAGTTGA+AGG | 0.427104 | tig0016338:-69103 | Msa1358470:CDS |
| CATTGCTATGTTAGTAGCTG+AGG | 0.547311 | tig0016338:-69214 | Msa1358470:CDS |
| TGGTGCTGCTGTAGAAATAT+GGG | 0.550057 | tig0016338:-69151 | Msa1358470:CDS |
| ACTGGTTTCCAAGTTGAAGG+AGG | 0.631072 | tig0016338:-69100 | Msa1358470:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTCAAGAGTTTAAAAAATGC+TGG | - | tig0016338:69062-69081 | Msa1358470:CDS | 30.0% | |
| AAGAGTTTAAAAAATGCTGG+TGG | - | tig0016338:69065-69084 | Msa1358470:CDS | 30.0% | |
| ! | GCAATAGCAAATTGAGTTTT+GGG | + | tig0016338:69197-69216 | None:intergenic | 30.0% |
| ! | AGCAATAGCAAATTGAGTTT+TGG | + | tig0016338:69198-69217 | None:intergenic | 30.0% |
| AGTTTAAAAAATGCTGGTGG+TGG | - | tig0016338:69068-69087 | Msa1358470:CDS | 35.0% | |
| !! | GTCTTCTTGTTATTCACTAC+TGG | - | tig0016338:69121-69140 | Msa1358470:CDS | 35.0% |
| CATTGCTATGTTAGTAGCTG+AGG | - | tig0016338:69025-69044 | Msa1358470:CDS | 40.0% | |
| !! | TGGTGCTGCTGTAGAAATAT+GGG | - | tig0016338:69088-69107 | Msa1358470:CDS | 40.0% |
| !! | ACTACTGGTTTCCAAGTTGA+AGG | - | tig0016338:69136-69155 | Msa1358470:CDS | 40.0% |
| !! | GCAACTTGTTCAGAGTGTTT+TGG | - | tig0016338:69169-69188 | Msa1358470:CDS | 40.0% |
| !! | GTGGTGCTGCTGTAGAAATA+TGG | - | tig0016338:69087-69106 | Msa1358470:CDS | 45.0% |
| ! | ACTGGTTTCCAAGTTGAAGG+AGG | - | tig0016338:69139-69158 | Msa1358470:CDS | 45.0% |
| CTTCTTCTCCTCCTTCAACT+TGG | + | tig0016338:69150-69169 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0016338 | gene | 69017 | 69244 | 69017 | ID=Msa1358470;Name=Msa1358470 |
| tig0016338 | mRNA | 69017 | 69244 | 69017 | ID=Msa1358470-mRNA-1;Parent=Msa1358470;Name=Msa1358470-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|75 |
| tig0016338 | exon | 69017 | 69244 | 69017 | ID=Msa1358470-mRNA-1:exon:10873;Parent=Msa1358470-mRNA-1 |
| tig0016338 | CDS | 69017 | 69244 | 69017 | ID=Msa1358470-mRNA-1:cds;Parent=Msa1358470-mRNA-1 |
| Gene Sequence |
| Protein sequence |
