Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1392020 | RYR03716.1 | 86.667 | 45 | 6 | 0 | 1 | 45 | 1 | 45 | 8.20e-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1392020 | A0A444YP21 | 86.667 | 45 | 6 | 0 | 1 | 45 | 1 | 45 | 3.92e-18 | 81.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1392010 | Msa1392020 | 0.829970 | 3.819896e-55 | -8.615850e-47 |
| Msa1392020 | Msa1392160 | 0.806395 | 8.400702e-50 | -8.615850e-47 |
| Msa0983190 | Msa1392020 | 0.817725 | 2.841488e-52 | -8.615850e-47 |
| Msa0983310 | Msa1392020 | 0.972837 | 2.018827e-135 | -8.615850e-47 |
| Msa0983320 | Msa1392020 | 0.831659 | 1.472587e-55 | -8.615850e-47 |
| Msa0983520 | Msa1392020 | 0.817673 | 2.918854e-52 | -8.615850e-47 |
| Msa0908850 | Msa1392020 | 0.813411 | 2.595154e-51 | -8.615850e-47 |
| Msa0944900 | Msa1392020 | 0.804064 | 2.584760e-49 | -8.615850e-47 |
| Msa0944920 | Msa1392020 | 0.820912 | 5.336450e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCGCCCCGGGACGGGAGT+GGG | 0.361835 | tig0021468:-45595 | Msa1392020:CDS |
| CGCGCAGGCAACAGCAGCAC+CGG | 0.381366 | tig0021468:-45562 | Msa1392020:CDS |
| ACTCCCGTCCCGGGGCGCTA+AGG | 0.393401 | tig0021468:+45598 | None:intergenic |
| TATTTATTGTGGCTATATGT+TGG | 0.425913 | tig0021468:-45431 | Msa1392020:CDS |
| AGCGCCCCGGGACGGGAGTG+GGG | 0.428726 | tig0021468:-45594 | Msa1392020:CDS |
| CTGCTTCTTCTTATTTATTG+TGG | 0.439184 | tig0021468:-45442 | Msa1392020:CDS |
| GCTGCTGTTGCCTGCGCGTA+GGG | 0.444552 | tig0021468:+45567 | None:intergenic |
| CGCAACAAAAGCTTGATTCA+AGG | 0.460458 | tig0021468:-45661 | Msa1392020:CDS |
| CAAAAGCCCCTTAGCGCCCC+GGG | 0.475074 | tig0021468:-45606 | Msa1392020:CDS |
| CACAATAAATAAGAAGAAGC+AGG | 0.506204 | tig0021468:+45443 | None:intergenic |
| TGCTGCTGTTGCCTGCGCGT+AGG | 0.513689 | tig0021468:+45566 | None:intergenic |
| ACAAAAGCCCCTTAGCGCCC+CGG | 0.520929 | tig0021468:-45607 | Msa1392020:CDS |
| AGATTCTGACTTCGTAGAGC+CGG | 0.543209 | tig0021468:+45543 | None:intergenic |
| ATTGTGGCTATATGTTGGCG+TGG | 0.693941 | tig0021468:-45426 | Msa1392020:CDS |
| GTGGCTATATGTTGGCGTGG+CGG | 0.707120 | tig0021468:-45423 | Msa1392020:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTTATTCACGAATGAATT+GGG | + | tig0021468:45554-45573 | None:intergenic | 25.0% |
| ! | ATTCTTATTCACGAATGAAT+TGG | + | tig0021468:45555-45574 | None:intergenic | 25.0% |
| ! | ATTCGTGAATAAGAATTCAA+GGG | - | tig0021468:45559-45578 | Msa1392020:CDS | 25.0% |
| ! | TATTTATTGTGGCTATATGT+TGG | - | tig0021468:45622-45641 | Msa1392020:CDS | 25.0% |
| TCAGAATCTAATCTTTGTGT+TGG | - | tig0021468:45524-45543 | Msa1392020:intron | 30.0% | |
| CATTCGTGAATAAGAATTCA+AGG | - | tig0021468:45558-45577 | Msa1392020:CDS | 30.0% | |
| CACAATAAATAAGAAGAAGC+AGG | + | tig0021468:45613-45632 | None:intergenic | 30.0% | |
| ! | CTGCTTCTTCTTATTTATTG+TGG | - | tig0021468:45611-45630 | Msa1392020:CDS | 30.0% |
| !!! | GCTTTTGTTTTTGGAACAGA+CGG | + | tig0021468:45434-45453 | None:intergenic | 35.0% |
| CGCAACAAAAGCTTGATTCA+AGG | - | tig0021468:45392-45411 | Msa1392020:CDS | 40.0% | |
| !!! | CGCTAAGGGGCTTTTGTTTT+TGG | + | tig0021468:45443-45462 | None:intergenic | 45.0% |
| AGATTCTGACTTCGTAGAGC+CGG | + | tig0021468:45513-45532 | None:intergenic | 45.0% | |
| ATTGTGGCTATATGTTGGCG+TGG | - | tig0021468:45627-45646 | Msa1392020:CDS | 45.0% | |
| GTGGCTATATGTTGGCGTGG+CGG | - | tig0021468:45630-45649 | Msa1392020:CDS | 55.0% | |
| ACAAAAGCCCCTTAGCGCCC+CGG | - | tig0021468:45446-45465 | Msa1392020:CDS | 60.0% | |
| CAAAAGCCCCTTAGCGCCCC+GGG | - | tig0021468:45447-45466 | Msa1392020:CDS | 65.0% | |
| GCTGCTGTTGCCTGCGCGTA+GGG | + | tig0021468:45489-45508 | None:intergenic | 65.0% | |
| TGCTGCTGTTGCCTGCGCGT+AGG | + | tig0021468:45490-45509 | None:intergenic | 65.0% | |
| CGCGCAGGCAACAGCAGCAC+CGG | - | tig0021468:45491-45510 | Msa1392020:intron | 70.0% | |
| AGCCCCTTAGCGCCCCGGGA+CGG | - | tig0021468:45451-45470 | Msa1392020:CDS | 75.0% | |
| ! | TCCCGTCCCGGGGCGCTAAG+GGG | + | tig0021468:45456-45475 | None:intergenic | 75.0% |
| ! | CTCCCGTCCCGGGGCGCTAA+GGG | + | tig0021468:45457-45476 | None:intergenic | 75.0% |
| ! | ACTCCCGTCCCGGGGCGCTA+AGG | + | tig0021468:45458-45477 | None:intergenic | 75.0% |
| TTAGCGCCCCGGGACGGGAG+TGG | - | tig0021468:45457-45476 | Msa1392020:CDS | 75.0% | |
| TAGCGCCCCGGGACGGGAGT+GGG | - | tig0021468:45458-45477 | Msa1392020:CDS | 75.0% | |
| TGTCCCCCACTCCCGTCCCG+GGG | + | tig0021468:45466-45485 | None:intergenic | 75.0% | |
| GTGTCCCCCACTCCCGTCCC+GGG | + | tig0021468:45467-45486 | None:intergenic | 75.0% | |
| GGTGTCCCCCACTCCCGTCC+CGG | + | tig0021468:45468-45487 | None:intergenic | 75.0% | |
| GTGGGGGACACCCTACGCGC+AGG | - | tig0021468:45476-45495 | Msa1392020:intron | 75.0% | |
| ! | GCCCCTTAGCGCCCCGGGAC+GGG | - | tig0021468:45452-45471 | Msa1392020:CDS | 80.0% |
| ! | AGCGCCCCGGGACGGGAGTG+GGG | - | tig0021468:45459-45478 | Msa1392020:CDS | 80.0% |
| ! | GCGCCCCGGGACGGGAGTGG+GGG | - | tig0021468:45460-45479 | Msa1392020:CDS | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021468 | gene | 45388 | 45687 | 45388 | ID=Msa1392020;Name=Msa1392020 |
| tig0021468 | mRNA | 45388 | 45687 | 45388 | ID=Msa1392020-mRNA-1;Parent=Msa1392020;Name=Msa1392020-mRNA-1;_AED=0.84;_eAED=1.00;_QI=0|0|0|0.5|0|0|2|0|78 |
| tig0021468 | exon | 45553 | 45687 | 45553 | ID=Msa1392020-mRNA-1:exon:13028;Parent=Msa1392020-mRNA-1 |
| tig0021468 | exon | 45388 | 45489 | 45388 | ID=Msa1392020-mRNA-1:exon:13027;Parent=Msa1392020-mRNA-1 |
| tig0021468 | CDS | 45553 | 45687 | 45553 | ID=Msa1392020-mRNA-1:cds;Parent=Msa1392020-mRNA-1 |
| tig0021468 | CDS | 45388 | 45489 | 45388 | ID=Msa1392020-mRNA-1:cds;Parent=Msa1392020-mRNA-1 |
| Gene Sequence |
| Protein sequence |
