AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0944900


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0944900 MtrunA17_MTg0491251 98.544 206 3 0 1 206 8 213 9.43e-147 407
Msa0944900 MtrunA17_Chr7g0231341 95.455 176 8 0 31 206 6 181 1.65e-120 340
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 70 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTACTCGGCTCGGGTTC+GGG 0.146792 6_3:+22369712 Msa0944900:CDS
AAGCCGACGCCGACCCATTT+TGG 0.210676 6_3:-22369896 None:intergenic
CTCTTTGGATGTATCCTTCT+GGG 0.227156 6_3:+22369796 Msa0944900:CDS
CCTCTTTGGATGTATCCTTC+TGG 0.235353 6_3:+22369795 Msa0944900:CDS
AGACTTCGTTGCAAATAAAT+TGG 0.238736 6_3:+22370016 Msa0944900:CDS
CCCGCCGCTCCTATCCAAAA+TGG 0.239934 6_3:+22369882 Msa0944900:CDS
CTCTTTACTCGGCTCGGGTT+CGG 0.247183 6_3:+22369711 Msa0944900:CDS
TCAAAGAATGTCTAATTCTT+TGG 0.276750 6_3:+22369578 None:intergenic
TCAATAGAAATGCCAAAATA+AGG 0.312304 6_3:-22369626 None:intergenic
CGTAGCGCTTGCTCATAAAT+TGG 0.318064 6_3:-22370062 None:intergenic
AACCTCCTCTTTACTCGGCT+CGG 0.370554 6_3:+22369705 Msa0944900:CDS
ATTGGACCCTGCGGCTGCCC+CGG 0.373068 6_3:-22370044 None:intergenic
GATAGGAGCGGCGGGTCCGC+CGG 0.375170 6_3:-22369874 None:intergenic
GTTTAGAGACCTGCTGAAAA+AGG 0.379768 6_3:-22369685 None:intergenic
TATCCTTCTGGGGCTGATGC+TGG 0.380202 6_3:+22369807 Msa0944900:CDS
AGCGGCGGGTCCGCCGGGGC+GGG 0.390016 6_3:-22369868 None:intergenic
TATGGTTTCGCGGCCGGAGC+TGG 0.394590 6_3:-22369939 None:intergenic
GGGAAGGCTGATGCTGCTCT+TGG 0.402499 6_3:-22369848 None:intergenic
GCGGGTCCGCCGGGGCGGGA+AGG 0.403262 6_3:-22369864 None:intergenic
CTTTACTCGGCTCGGGTTCG+GGG 0.407970 6_3:+22369713 Msa0944900:CDS
GTGCCGAGGAAATCCCTCTT+TGG 0.408347 6_3:+22369781 Msa0944900:CDS
TTGGACCCTGCGGCTGCCCC+GGG 0.438206 6_3:-22370043 None:intergenic
GGAAGACCCGGGGCAGCCGC+AGG 0.439885 6_3:+22370037 Msa0944900:CDS
CCACTTTACTTTCAAAAGAT+CGG 0.450190 6_3:-22369661 None:intergenic
ACCTCCTCTTTACTCGGCTC+GGG 0.456204 6_3:+22369706 Msa0944900:CDS
TAGGAGCGGCGGGTCCGCCG+GGG 0.472146 6_3:-22369872 None:intergenic
TTCCGTGGAGCAGCCAGCTC+CGG 0.472407 6_3:+22369926 Msa0944900:CDS
GGCCGGAGCTGGCTGCTCCA+CGG 0.474217 6_3:-22369928 None:intergenic
CTCTAAACCTCCTCTTTACT+CGG 0.475617 6_3:+22369700 Msa0944900:CDS
TGCAAATAAATTGGAAGACC+CGG 0.488016 6_3:+22370025 Msa0944900:CDS
CAGCATCAGCCTTCCCGCCC+CGG 0.489074 6_3:+22369855 Msa0944900:CDS
CCGCCGCTCCTATCCAAAAT+GGG 0.493631 6_3:+22369883 Msa0944900:CDS
GGACGAACTTCAGCGCTGGC+TGG 0.506906 6_3:+22370127 Msa0944900:CDS
AGCGCTGGCTGGTTTCACTA+AGG 0.509513 6_3:+22370138 Msa0944900:CDS
AATACGATTGCCAAGGCGGC+CGG 0.515316 6_3:-22369738 None:intergenic
AGCTGAAGGAGGGCTATGTA+TGG 0.518841 6_3:-22369957 None:intergenic
CGAACCCGAGCCGAGTAAAG+AGG 0.520061 6_3:-22369710 None:intergenic
GCGCTGGCTGGTTTCACTAA+GGG 0.521276 6_3:+22370139 Msa0944900:CDS
GCTATGTATGGTTTCGCGGC+CGG 0.522221 6_3:-22369945 None:intergenic
GAGGGCTATGTATGGTTTCG+CGG 0.534222 6_3:-22369949 None:intergenic
AGCAAGCGCTACGCCTGGTA+TGG 0.535586 6_3:+22370072 Msa0944900:CDS
TTTACTCGGCTCGGGTTCGG+GGG 0.543290 6_3:+22369714 Msa0944900:CDS
TTATGAGCAAGCGCTACGCC+TGG 0.557111 6_3:+22370067 Msa0944900:CDS
CGCTCCTATCCAAAATGGGT+CGG 0.568858 6_3:+22369887 Msa0944900:CDS
GATCGATGGCAGTACTAACC+AGG 0.569954 6_3:+22370106 Msa0944900:CDS
GCAAATAAATTGGAAGACCC+GGG 0.573068 6_3:+22370026 Msa0944900:CDS
ATAGCCCTCCTTCAGCTAGA+GGG 0.580354 6_3:+22369963 Msa0944900:CDS
ACCAGGACGAACTTCAGCGC+TGG 0.584629 6_3:+22370123 Msa0944900:CDS
GAAGACCCGGGGCAGCCGCA+GGG 0.585751 6_3:+22370038 Msa0944900:CDS
GCCAGCGCTGAAGTTCGTCC+TGG 0.586980 6_3:-22370124 None:intergenic
TCTTTGGATGTATCCTTCTG+GGG 0.587601 6_3:+22369797 Msa0944900:CDS
TGGCTGCTCCACGGAACTAG+TGG 0.588921 6_3:-22369919 None:intergenic
AAGAAATACGATTGCCAAGG+CGG 0.590719 6_3:-22369742 None:intergenic
CAAAAGAAATACGATTGCCA+AGG 0.602322 6_3:-22369745 None:intergenic
AAAATGGATTTGTATTGTCG+GGG 0.604607 6_3:-22370167 None:intergenic
TCTCGAGTTCATACCATACC+AGG 0.605945 6_3:-22370085 None:intergenic
TGGTATGAACTCGAGATCGA+TGG 0.622194 6_3:+22370092 Msa0944900:CDS
CAGAAGGATACATCCAAAGA+GGG 0.626664 6_3:-22369794 None:intergenic
GCGAGCACTATTCAGTGCCG+AGG 0.627117 6_3:+22369767 Msa0944900:CDS
TAGCCCTCCTTCAGCTAGAG+GGG 0.628752 6_3:+22369964 Msa0944900:CDS
CATCCAAAGAGGGATTTCCT+CGG 0.631260 6_3:-22369784 None:intergenic
TATCCAAAATGGGTCGGCGT+CGG 0.631571 6_3:+22369893 Msa0944900:CDS
GAGCCAGCATCAGCCCCAGA+AGG 0.632838 6_3:-22369810 None:intergenic
CCAGAAGGATACATCCAAAG+AGG 0.635005 6_3:-22369795 None:intergenic
TGCTCATAAATTGGACCCTG+CGG 0.637696 6_3:-22370053 None:intergenic
CATAGCCCTCCTTCAGCTAG+AGG 0.642446 6_3:+22369962 Msa0944900:CDS
AGCCCTCCTTCAGCTAGAGG+GGG 0.644919 6_3:+22369965 Msa0944900:CDS
AAATGGATTTGTATTGTCGG+GGG 0.651927 6_3:-22370166 None:intergenic
ACCCGAGCCGAGTAAAGAGG+AGG 0.667486 6_3:-22369707 None:intergenic
CAAATAAATTGGAAGACCCG+GGG 0.684347 6_3:+22370027 Msa0944900:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTTTTCTTCCTTATTT+TGG + chr6_3:22369614-22369633 Msa0944900:CDS 20.0%
! TCAATAGAAATGCCAAAATA+AGG - chr6_3:22369629-22369648 None:intergenic 25.0%
!! AAAAATGGATTTGTATTGTC+GGG - chr6_3:22370171-22370190 None:intergenic 25.0%
!! CAAAAATGGATTTGTATTGT+CGG - chr6_3:22370172-22370191 None:intergenic 25.0%
CCACTTTACTTTCAAAAGAT+CGG - chr6_3:22369664-22369683 None:intergenic 30.0%
AGACTTCGTTGCAAATAAAT+TGG + chr6_3:22370016-22370035 Msa0944900:CDS 30.0%
! AAAATGGATTTGTATTGTCG+GGG - chr6_3:22370170-22370189 None:intergenic 30.0%
! CGACAATACAAATCCATTTT+TGG + chr6_3:22370170-22370189 Msa0944900:CDS 30.0%
!! CCGATCTTTTGAAAGTAAAG+TGG + chr6_3:22369661-22369680 Msa0944900:CDS 35.0%
CAAAAGAAATACGATTGCCA+AGG - chr6_3:22369748-22369767 None:intergenic 35.0%
TGCAAATAAATTGGAAGACC+CGG + chr6_3:22370025-22370044 Msa0944900:CDS 35.0%
! AAATGGATTTGTATTGTCGG+GGG - chr6_3:22370169-22370188 None:intergenic 35.0%
!!! TAAAGTGGACCTTTTTCAGC+AGG + chr6_3:22369676-22369695 Msa0944900:CDS 40.0%
GTTTAGAGACCTGCTGAAAA+AGG - chr6_3:22369688-22369707 None:intergenic 40.0%
CTCTAAACCTCCTCTTTACT+CGG + chr6_3:22369700-22369719 Msa0944900:CDS 40.0%
AAGAAATACGATTGCCAAGG+CGG - chr6_3:22369745-22369764 None:intergenic 40.0%
CAGAAGGATACATCCAAAGA+GGG - chr6_3:22369797-22369816 None:intergenic 40.0%
CTCTTTGGATGTATCCTTCT+GGG + chr6_3:22369796-22369815 Msa0944900:CDS 40.0%
TCTTTGGATGTATCCTTCTG+GGG + chr6_3:22369797-22369816 Msa0944900:CDS 40.0%
GCAAATAAATTGGAAGACCC+GGG + chr6_3:22370026-22370045 Msa0944900:CDS 40.0%
CAAATAAATTGGAAGACCCG+GGG + chr6_3:22370027-22370046 Msa0944900:CDS 40.0%
!!! AATCCATTTTTGGCTTCTCC+CGG + chr6_3:22370180-22370199 Msa0944900:CDS 40.0%
CATCCAAAGAGGGATTTCCT+CGG - chr6_3:22369787-22369806 None:intergenic 45.0%
CCAGAAGGATACATCCAAAG+AGG - chr6_3:22369798-22369817 None:intergenic 45.0%
CCTCTTTGGATGTATCCTTC+TGG + chr6_3:22369795-22369814 Msa0944900:CDS 45.0%
TGCTCATAAATTGGACCCTG+CGG - chr6_3:22370056-22370075 None:intergenic 45.0%
CGTAGCGCTTGCTCATAAAT+TGG - chr6_3:22370065-22370084 None:intergenic 45.0%
TCTCGAGTTCATACCATACC+AGG - chr6_3:22370088-22370107 None:intergenic 45.0%
TGGTATGAACTCGAGATCGA+TGG + chr6_3:22370092-22370111 Msa0944900:CDS 45.0%
!!! ATCCATTTTTGGCTTCTCCC+GGG + chr6_3:22370181-22370200 Msa0944900:CDS 45.0%
AACCTCCTCTTTACTCGGCT+CGG + chr6_3:22369705-22369724 Msa0944900:CDS 50.0%
! CGACCCATTTTGGATAGGAG+CGG - chr6_3:22369889-22369908 None:intergenic 50.0%
CGCTCCTATCCAAAATGGGT+CGG + chr6_3:22369887-22369906 Msa0944900:CDS 50.0%
TATCCAAAATGGGTCGGCGT+CGG + chr6_3:22369893-22369912 Msa0944900:CDS 50.0%
GAGGGCTATGTATGGTTTCG+CGG - chr6_3:22369952-22369971 None:intergenic 50.0%
AGCTGAAGGAGGGCTATGTA+TGG - chr6_3:22369960-22369979 None:intergenic 50.0%
ATAGCCCTCCTTCAGCTAGA+GGG + chr6_3:22369963-22369982 Msa0944900:CDS 50.0%
GATCGATGGCAGTACTAACC+AGG + chr6_3:22370106-22370125 Msa0944900:CDS 50.0%
!!! TCCATTTTTGGCTTCTCCCG+GGG + chr6_3:22370182-22370201 Msa0944900:CDS 50.0%
ACCTCCTCTTTACTCGGCTC+GGG + chr6_3:22369706-22369725 Msa0944900:CDS 55.0%
!! CTCTTTACTCGGCTCGGGTT+CGG + chr6_3:22369711-22369730 Msa0944900:CDS 55.0%
!! TCTTTACTCGGCTCGGGTTC+GGG + chr6_3:22369712-22369731 Msa0944900:CDS 55.0%
AATACGATTGCCAAGGCGGC+CGG - chr6_3:22369741-22369760 None:intergenic 55.0%
GTGCCGAGGAAATCCCTCTT+TGG + chr6_3:22369781-22369800 Msa0944900:CDS 55.0%
!! TATCCTTCTGGGGCTGATGC+TGG + chr6_3:22369807-22369826 Msa0944900:CDS 55.0%
! CCATTTTGGATAGGAGCGGC+GGG - chr6_3:22369885-22369904 None:intergenic 55.0%
! CCCATTTTGGATAGGAGCGG+CGG - chr6_3:22369886-22369905 None:intergenic 55.0%
CCGCCGCTCCTATCCAAAAT+GGG + chr6_3:22369883-22369902 Msa0944900:CDS 55.0%
! GACGCCGACCCATTTTGGAT+AGG - chr6_3:22369894-22369913 None:intergenic 55.0%
GCTATGTATGGTTTCGCGGC+CGG - chr6_3:22369948-22369967 None:intergenic 55.0%
CATAGCCCTCCTTCAGCTAG+AGG + chr6_3:22369962-22369981 Msa0944900:CDS 55.0%
TAGCCCTCCTTCAGCTAGAG+GGG + chr6_3:22369964-22369983 Msa0944900:CDS 55.0%
TTATGAGCAAGCGCTACGCC+TGG + chr6_3:22370067-22370086 Msa0944900:CDS 55.0%
!! AGCGCTGGCTGGTTTCACTA+AGG + chr6_3:22370138-22370157 Msa0944900:CDS 55.0%
!! GCGCTGGCTGGTTTCACTAA+GGG + chr6_3:22370139-22370158 Msa0944900:CDS 55.0%
!!! CCATTTTTGGCTTCTCCCGG+GGG + chr6_3:22370183-22370202 Msa0944900:CDS 55.0%
!!! CATTTTTGGCTTCTCCCGGG+GGG + chr6_3:22370184-22370203 Msa0944900:CDS 55.0%
ACCCGAGCCGAGTAAAGAGG+AGG - chr6_3:22369710-22369729 None:intergenic 60.0%
CGAACCCGAGCCGAGTAAAG+AGG - chr6_3:22369713-22369732 None:intergenic 60.0%
!! CTTTACTCGGCTCGGGTTCG+GGG + chr6_3:22369713-22369732 Msa0944900:CDS 60.0%
!! TTTACTCGGCTCGGGTTCGG+GGG + chr6_3:22369714-22369733 Msa0944900:CDS 60.0%
GCGAGCACTATTCAGTGCCG+AGG + chr6_3:22369767-22369786 Msa0944900:CDS 60.0%
GGGAAGGCTGATGCTGCTCT+TGG - chr6_3:22369851-22369870 None:intergenic 60.0%
CCCGCCGCTCCTATCCAAAA+TGG + chr6_3:22369882-22369901 Msa0944900:CDS 60.0%
! AAGCCGACGCCGACCCATTT+TGG - chr6_3:22369899-22369918 None:intergenic 60.0%
! GTCGGCTTCCACTAGTTCCG+TGG + chr6_3:22369911-22369930 Msa0944900:CDS 60.0%
TGGCTGCTCCACGGAACTAG+TGG - chr6_3:22369922-22369941 None:intergenic 60.0%
AGCCCTCCTTCAGCTAGAGG+GGG + chr6_3:22369965-22369984 Msa0944900:CDS 60.0%
CTCCCCCTCTAGCTGAAGGA+GGG - chr6_3:22369970-22369989 None:intergenic 60.0%
TCCTTCAGCTAGAGGGGGAG+CGG + chr6_3:22369970-22369989 Msa0944900:CDS 60.0%
AGCAAGCGCTACGCCTGGTA+TGG + chr6_3:22370072-22370091 Msa0944900:CDS 60.0%
ACCAGGACGAACTTCAGCGC+TGG + chr6_3:22370123-22370142 Msa0944900:CDS 60.0%
CCCCCGGGAGAAGCCAAAAA+TGG - chr6_3:22370186-22370205 None:intergenic 60.0%
GAGCCAGCATCAGCCCCAGA+AGG - chr6_3:22369813-22369832 None:intergenic 65.0%
TTCCGTGGAGCAGCCAGCTC+CGG + chr6_3:22369926-22369945 Msa0944900:CDS 65.0%
TATGGTTTCGCGGCCGGAGC+TGG - chr6_3:22369942-22369961 None:intergenic 65.0%
GCTCCCCCTCTAGCTGAAGG+AGG - chr6_3:22369971-22369990 None:intergenic 65.0%
TCCGCTCCCCCTCTAGCTGA+AGG - chr6_3:22369974-22369993 None:intergenic 65.0%
! GCCAGCGCTGAAGTTCGTCC+TGG - chr6_3:22370127-22370146 None:intergenic 65.0%
!! GGACGAACTTCAGCGCTGGC+TGG + chr6_3:22370127-22370146 Msa0944900:CDS 65.0%
CAGCATCAGCCTTCCCGCCC+CGG + chr6_3:22369855-22369874 Msa0944900:CDS 70.0%
ATTGGACCCTGCGGCTGCCC+CGG - chr6_3:22370047-22370066 None:intergenic 70.0%
!! TCGGCTCGGGTTCGGGGGTC+CGG + chr6_3:22369719-22369738 Msa0944900:CDS 75.0%
CATCAGCCTTCCCGCCCCGG+CGG + chr6_3:22369858-22369877 Msa0944900:CDS 75.0%
ATAGGAGCGGCGGGTCCGCC+GGG - chr6_3:22369876-22369895 None:intergenic 75.0%
GATAGGAGCGGCGGGTCCGC+CGG - chr6_3:22369877-22369896 None:intergenic 75.0%
GGCCGGAGCTGGCTGCTCCA+CGG - chr6_3:22369931-22369950 None:intergenic 75.0%
GAAGACCCGGGGCAGCCGCA+GGG + chr6_3:22370038-22370057 Msa0944900:CDS 75.0%
TTGGACCCTGCGGCTGCCCC+GGG - chr6_3:22370046-22370065 None:intergenic 75.0%
! GTTCGGGGGTCCGGCCGCCT+TGG + chr6_3:22369728-22369747 Msa0944900:CDS 80.0%
! TAGGAGCGGCGGGTCCGCCG+GGG - chr6_3:22369875-22369894 None:intergenic 80.0%
! GGAAGACCCGGGGCAGCCGC+AGG + chr6_3:22370037-22370056 Msa0944900:CDS 80.0%
! GCGGGTCCGCCGGGGCGGGA+AGG - chr6_3:22369867-22369886 None:intergenic 90.0%
! AGCGGCGGGTCCGCCGGGGC+GGG - chr6_3:22369871-22369890 None:intergenic 90.0%
! GAGCGGCGGGTCCGCCGGGG+CGG - chr6_3:22369872-22369891 None:intergenic 90.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 22369585 22370217 22369585 ID=Msa0944900;Name=Msa0944900
chr6_3 mRNA 22369585 22370217 22369585 ID=Msa0944900-mRNA-1;Parent=Msa0944900;Name=Msa0944900-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|210
chr6_3 exon 22369585 22370217 22369585 ID=Msa0944900-mRNA-1:exon:6558;Parent=Msa0944900-mRNA-1
chr6_3 CDS 22369585 22370217 22369585 ID=Msa0944900-mRNA-1:cds;Parent=Msa0944900-mRNA-1
Gene Sequence

>Msa0944900

ATGTCTAATTCTTTGGTAAGAATTTCTATCATTTTTTTCTTCCTTATTTTGGCATTTCTATTGATTGACGTTGATTCCGATCTTTTGAAAGTAAAGTGGACCTTTTTCAGCAGGTCTCTAAACCTCCTCTTTACTCGGCTCGGGTTCGGGGGTCCGGCCGCCTTGGCAATCGTATTTCTTTTGCGAGCACTATTCAGTGCCGAGGAAATCCCTCTTTGGATGTATCCTTCTGGGGCTGATGCTGGCTCAGAAGCAAGCGTAAACCAAGAGCAGCATCAGCCTTCCCGCCCCGGCGGACCCGCCGCTCCTATCCAAAATGGGTCGGCGTCGGCTTCCACTAGTTCCGTGGAGCAGCCAGCTCCGGCCGCGAAACCATACATAGCCCTCCTTCAGCTAGAGGGGGAGCGGAAACGTCTTATCGACGATATTGTAGACTTCGTTGCAAATAAATTGGAAGACCCGGGGCAGCCGCAGGGTCCAATTTATGAGCAAGCGCTACGCCTGGTATGGTATGAACTCGAGATCGATGGCAGTACTAACCAGGACGAACTTCAGCGCTGGCTGGTTTCACTAAGGGAAAACCCCCGACAATACAAATCCATTTTTGGCTTCTCCCGGGGGGATTTTTTATGA

Protein sequence

>Msa0944900

MSNSLVRISIIFFFLILAFLLIDVDSDLLKVKWTFFSRSLNLLFTRLGFGGPAALAIVFLLRALFSAEEIPLWMYPSGADAGSEASVNQEQHQPSRPGGPAAPIQNGSASASTSSVEQPAPAAKPYIALLQLEGERKRLIDDIVDFVANKLEDPGQPQGPIYEQALRLVWYELEIDGSTNQDELQRWLVSLRENPRQYKSIFGFSRGDFL*