AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0983320


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTGCTGTGGGTCAGTTTC+GGG 0.191312 6_4:+26440051 Msa0983320:CDS
TGTGGGTCAGTTTCGGGGCG+GGG 0.313334 6_4:+26440057 Msa0983320:CDS
CTTTCTGTGTGGGACGATGC+CGG 0.316270 6_4:-26439606 None:intergenic
GCTGTGCTGTGGGTCAGTTT+CGG 0.325201 6_4:+26440050 Msa0983320:CDS
GTGGGTCAGTTTCGGGGCGG+GGG 0.330602 6_4:+26440058 Msa0983320:CDS
CTGTGGGTCAGTTTCGGGGC+GGG 0.351980 6_4:+26440056 Msa0983320:CDS
CGCCCATTCGCTTCGCGCGC+GGG 0.358241 6_4:-26440028 None:intergenic
TGACTTCGGTTCGCCAGTTC+AGG 0.360274 6_4:-26439693 None:intergenic
TTCCCGCGCGCGAAGCGAAT+GGG 0.362487 6_4:+26440026 Msa0983320:CDS
TTTGCGCTGCGCGTTCCCGC+TGG 0.368645 6_4:-26439756 None:intergenic
TTTCTGTGTGGGACGATGCC+GGG 0.378127 6_4:-26439605 None:intergenic
CGCGGGAAGGCTACGAAGTT+CGG 0.381304 6_4:-26440011 None:intergenic
CTCGGTCCCCGATGGCCTGC+GGG 0.387846 6_4:-26439805 None:intergenic
CTTCCCGCGCGCGAAGCGAA+TGG 0.392844 6_4:+26440025 Msa0983320:CDS
CCGTCGCCTACTTACCGTTT+AGG 0.396921 6_4:+26439915 Msa0983320:CDS
ACGAAGTTCCTTCCCTTAGC+TGG 0.398339 6_4:-26439953 None:intergenic
TGGCGAACCGAAGTCACTTT+CGG 0.415405 6_4:+26439700 Msa0983320:CDS
GCGCCCATTCGCTTCGCGCG+CGG 0.425723 6_4:-26440029 None:intergenic
AGCGAATGGGCGCTGTGCTG+TGG 0.429564 6_4:+26440039 Msa0983320:CDS
CATTCGCTTCGCGCGCGGGA+AGG 0.433191 6_4:-26440024 None:intergenic
TGGGTCAGTTTCGGGGCGGG+GGG 0.448710 6_4:+26440059 Msa0983320:CDS
GCTGCGCGTTCCCGCTGGAC+TGG 0.454225 6_4:-26439751 None:intergenic
AAGAGAACTGAGCTGGCGCT+TGG 0.459565 6_4:-26439488 None:intergenic
CGTGTTAGCTGTAGGAAGTA+TGG 0.472037 6_4:-26439725 None:intergenic
GCGAGGAGTTGAGCCTGAAC+TGG 0.476860 6_4:+26439680 Msa0983320:intron
GGGACGATGCCGGGGAGAGA+AGG 0.483121 6_4:-26439596 None:intergenic
TCTCGCTGGTGCCACCTAAA+CGG 0.485900 6_4:-26439929 None:intergenic
TTCCCTTAGCTGGATCTCGC+TGG 0.493334 6_4:-26439943 None:intergenic
AGAGAACTGAGCTGGCGCTT+GGG 0.494487 6_4:-26439487 None:intergenic
GGACGATGCCGGGGAGAGAA+GGG 0.500282 6_4:-26439595 None:intergenic
GGCGACGGATGTGTGACGTT+TGG 0.502967 6_4:-26439900 None:intergenic
GACGCATTCCCTTCTCTCCC+CGG 0.520898 6_4:+26439587 Msa0983320:CDS
ATAAATCGATATGTATCTGA+TGG 0.523902 6_4:+26439442 None:intergenic
CTCCATCCATGACAGTGCAT+TGG 0.532650 6_4:-26439521 None:intergenic
GTCCAATGCACTGTCATGGA+TGG 0.536192 6_4:+26439519 Msa0983320:CDS
GCGAATGGGCGCTGTGCTGT+GGG 0.539770 6_4:+26440040 Msa0983320:CDS
AGCTCAGTTCTCTTATCCAT+CGG 0.540408 6_4:+26439497 Msa0983320:CDS
CAATGCACTGTCATGGATGG+AGG 0.543873 6_4:+26439522 Msa0983320:CDS
TTCATGAGACCGCGGCGGAG+TGG 0.545398 6_4:-26439988 None:intergenic
ACTCGGTCCCCGATGGCCTG+CGG 0.553396 6_4:-26439806 None:intergenic
ATCGGTCCAATGCACTGTCA+TGG 0.554798 6_4:+26439515 Msa0983320:CDS
GCTGTGGGTCAGTTTCGGGG+CGG 0.555599 6_4:+26440055 Msa0983320:CDS
GATGGATAAGAGAACTGAGC+TGG 0.556532 6_4:-26439495 None:intergenic
ATGACAGTGCATTGGACCGA+TGG 0.558632 6_4:-26439513 None:intergenic
TTCTGTGTGGGACGATGCCG+GGG 0.559319 6_4:-26439604 None:intergenic
CCTTACCACTCGGTCCCCGA+TGG 0.572919 6_4:-26439813 None:intergenic
TCGGTCCCCGATGGCCTGCG+GGG 0.576019 6_4:-26439804 None:intergenic
TATGGTTCCGAAAGTGACTT+CGG 0.579844 6_4:-26439707 None:intergenic
ATCCAAATGAATCATTCTTG+TGG 0.586529 6_4:-26440092 None:intergenic
CGGTCCCCGATGGCCTGCGG+GGG 0.603794 6_4:-26439803 None:intergenic
TAACACGTGTCCAGTCCAGC+GGG 0.606235 6_4:+26439741 Msa0983320:CDS
GTGCCACCTAAACGGTAAGT+AGG 0.609035 6_4:-26439921 None:intergenic
TGTGCTGTGGGTCAGTTTCG+GGG 0.610535 6_4:+26440052 Msa0983320:CDS
AGCGAGATCCAGCTAAGGGA+AGG 0.613371 6_4:+26439945 Msa0983320:CDS
GCACCAGCGAGATCCAGCTA+AGG 0.613771 6_4:+26439940 Msa0983320:CDS
CACCAGCGAGATCCAGCTAA+GGG 0.620544 6_4:+26439941 Msa0983320:CDS
AATGCACTGTCATGGATGGA+GGG 0.629806 6_4:+26439523 Msa0983320:CDS
ACTGGACACGTGTTAGCTGT+AGG 0.636729 6_4:-26439733 None:intergenic
TCGCCTACTTACCGTTTAGG+TGG 0.640918 6_4:+26439918 Msa0983320:CDS
GCGGCTGCTCCACTCCGCCG+CGG 0.642559 6_4:+26439979 Msa0983320:CDS
GGAGAGAAGGGAATGCGTCG+AGG 0.646607 6_4:-26439583 None:intergenic
GAGAAGGGAATGCGTCGAGG+CGG 0.663253 6_4:-26439580 None:intergenic
GCAGGCCATCGGGGACCGAG+TGG 0.669923 6_4:+26439808 Msa0983320:CDS
CTAACACGTGTCCAGTCCAG+CGG 0.686803 6_4:+26439740 Msa0983320:CDS
AGGGAAGGAACTTCGTGTCG+CGG 0.689775 6_4:+26439960 Msa0983320:CDS
CCTAAACGGTAAGTAGGCGA+CGG 0.699145 6_4:-26439915 None:intergenic
TTCGGTTCATGAGACCGCGG+CGG 0.744018 6_4:-26439993 None:intergenic
AAGTTCGGTTCATGAGACCG+CGG 0.751147 6_4:-26439996 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGCTCAGTTCTCTTATCCAT+CGG + chr6_4:26439497-26439516 Msa0983320:CDS 40.0%
TATGGTTCCGAAAGTGACTT+CGG - chr6_4:26439710-26439729 None:intergenic 40.0%
GGATCACTCATTCTTCCATT+GGG + chr6_4:26439855-26439874 Msa0983320:intron 40.0%
GATCACTCATTCTTCCATTG+GGG + chr6_4:26439856-26439875 Msa0983320:intron 40.0%
GATGGATAAGAGAACTGAGC+TGG - chr6_4:26439498-26439517 None:intergenic 45.0%
AATGCACTGTCATGGATGGA+GGG + chr6_4:26439523-26439542 Msa0983320:CDS 45.0%
TGCGCTCTTTCTTTCTGTGT+GGG - chr6_4:26439619-26439638 None:intergenic 45.0%
AACTCCTCGCTTTACGTTAG+CGG - chr6_4:26439670-26439689 None:intergenic 45.0%
CGTGTTAGCTGTAGGAAGTA+TGG - chr6_4:26439728-26439747 None:intergenic 45.0%
CGGATCACTCATTCTTCCAT+TGG + chr6_4:26439854-26439873 Msa0983320:intron 45.0%
ATGACAGTGCATTGGACCGA+TGG - chr6_4:26439516-26439535 None:intergenic 50.0%
ATCGGTCCAATGCACTGTCA+TGG + chr6_4:26439515-26439534 Msa0983320:CDS 50.0%
GTCCAATGCACTGTCATGGA+TGG + chr6_4:26439519-26439538 Msa0983320:CDS 50.0%
CTCCATCCATGACAGTGCAT+TGG - chr6_4:26439524-26439543 None:intergenic 50.0%
CAATGCACTGTCATGGATGG+AGG + chr6_4:26439522-26439541 Msa0983320:CDS 50.0%
CTGCGCTCTTTCTTTCTGTG+TGG - chr6_4:26439620-26439639 None:intergenic 50.0%
! TGGCGAACCGAAGTCACTTT+CGG + chr6_4:26439700-26439719 Msa0983320:CDS 50.0%
ACTGGACACGTGTTAGCTGT+AGG - chr6_4:26439736-26439755 None:intergenic 50.0%
CTCATTCTTCCATTGGGGAC+AGG + chr6_4:26439861-26439880 Msa0983320:intron 50.0%
CCTAAACGGTAAGTAGGCGA+CGG - chr6_4:26439918-26439937 None:intergenic 50.0%
TCGCCTACTTACCGTTTAGG+TGG + chr6_4:26439918-26439937 Msa0983320:CDS 50.0%
GTGCCACCTAAACGGTAAGT+AGG - chr6_4:26439924-26439943 None:intergenic 50.0%
ACGAAGTTCCTTCCCTTAGC+TGG - chr6_4:26439956-26439975 None:intergenic 50.0%
AAGTTCGGTTCATGAGACCG+CGG - chr6_4:26439999-26440018 None:intergenic 50.0%
AGAGAACTGAGCTGGCGCTT+GGG - chr6_4:26439490-26439509 None:intergenic 55.0%
AAGAGAACTGAGCTGGCGCT+TGG - chr6_4:26439491-26439510 None:intergenic 55.0%
TTTCTGTGTGGGACGATGCC+GGG - chr6_4:26439608-26439627 None:intergenic 55.0%
CTTTCTGTGTGGGACGATGC+CGG - chr6_4:26439609-26439628 None:intergenic 55.0%
! AAAGAAAGAGCGCAGAGCCC+CGG + chr6_4:26439625-26439644 Msa0983320:intron 55.0%
AGCGGACCTACACTACAGGT+CGG - chr6_4:26439652-26439671 None:intergenic 55.0%
! CGTTAGCGGACCTACACTAC+AGG - chr6_4:26439656-26439675 None:intergenic 55.0%
AGGTCCGCTAACGTAAAGCG+AGG + chr6_4:26439663-26439682 Msa0983320:intron 55.0%
! TGACTTCGGTTCGCCAGTTC+AGG - chr6_4:26439696-26439715 None:intergenic 55.0%
CTAACACGTGTCCAGTCCAG+CGG + chr6_4:26439740-26439759 Msa0983320:CDS 55.0%
TAACACGTGTCCAGTCCAGC+GGG + chr6_4:26439741-26439760 Msa0983320:CDS 55.0%
! CGGATCATGGCCTTACCACT+CGG - chr6_4:26439826-26439845 None:intergenic 55.0%
TGGTAAGGCCATGATCCGCA+GGG + chr6_4:26439828-26439847 Msa0983320:intron 55.0%
ATGAGTGATCCGTTCCCCTG+CGG - chr6_4:26439846-26439865 None:intergenic 55.0%
CCGTCGCCTACTTACCGTTT+AGG + chr6_4:26439915-26439934 Msa0983320:CDS 55.0%
!! TCTCGCTGGTGCCACCTAAA+CGG - chr6_4:26439932-26439951 None:intergenic 55.0%
CACCAGCGAGATCCAGCTAA+GGG + chr6_4:26439941-26439960 Msa0983320:CDS 55.0%
TTCCCTTAGCTGGATCTCGC+TGG - chr6_4:26439946-26439965 None:intergenic 55.0%
AGCGAGATCCAGCTAAGGGA+AGG + chr6_4:26439945-26439964 Msa0983320:CDS 55.0%
AGGGAAGGAACTTCGTGTCG+CGG + chr6_4:26439960-26439979 Msa0983320:CDS 55.0%
GCTGTGCTGTGGGTCAGTTT+CGG + chr6_4:26440050-26440069 Msa0983320:CDS 55.0%
CTGTGCTGTGGGTCAGTTTC+GGG + chr6_4:26440051-26440070 Msa0983320:CDS 55.0%
TGTGCTGTGGGTCAGTTTCG+GGG + chr6_4:26440052-26440071 Msa0983320:CDS 55.0%
GAGAAGGGAATGCGTCGAGG+CGG - chr6_4:26439583-26439602 None:intergenic 60.0%
GGAGAGAAGGGAATGCGTCG+AGG - chr6_4:26439586-26439605 None:intergenic 60.0%
GACGCATTCCCTTCTCTCCC+CGG + chr6_4:26439587-26439606 Msa0983320:CDS 60.0%
TTCTGTGTGGGACGATGCCG+GGG - chr6_4:26439607-26439626 None:intergenic 60.0%
ACCTACACTACAGGTCGGGC+CGG - chr6_4:26439647-26439666 None:intergenic 60.0%
GCGGACCTACACTACAGGTC+GGG - chr6_4:26439651-26439670 None:intergenic 60.0%
GCGAGGAGTTGAGCCTGAAC+TGG + chr6_4:26439680-26439699 Msa0983320:intron 60.0%
TGCTATACCGAATCCCCCGC+AGG + chr6_4:26439790-26439809 Msa0983320:CDS 60.0%
GTGGTAAGGCCATGATCCGC+AGG + chr6_4:26439827-26439846 Msa0983320:intron 60.0%
GGTAAGGCCATGATCCGCAG+GGG + chr6_4:26439829-26439848 Msa0983320:intron 60.0%
ATCCGTTCCCCTGCGGATCA+TGG - chr6_4:26439839-26439858 None:intergenic 60.0%
GTTCGTGCACCTGTCCCCAA+TGG - chr6_4:26439873-26439892 None:intergenic 60.0%
GGCGACGGATGTGTGACGTT+TGG - chr6_4:26439903-26439922 None:intergenic 60.0%
GCACCAGCGAGATCCAGCTA+AGG + chr6_4:26439940-26439959 Msa0983320:CDS 60.0%
TTCGGTTCATGAGACCGCGG+CGG - chr6_4:26439996-26440015 None:intergenic 60.0%
CGCGGGAAGGCTACGAAGTT+CGG - chr6_4:26440014-26440033 None:intergenic 60.0%
GGACGATGCCGGGGAGAGAA+GGG - chr6_4:26439598-26439617 None:intergenic 65.0%
CTACACTACAGGTCGGGCCG+GGG - chr6_4:26439645-26439664 None:intergenic 65.0%
CCTACACTACAGGTCGGGCC+GGG - chr6_4:26439646-26439665 None:intergenic 65.0%
CCTTACCACTCGGTCCCCGA+TGG - chr6_4:26439816-26439835 None:intergenic 65.0%
CCATCGGGGACCGAGTGGTA+AGG + chr6_4:26439813-26439832 Msa0983320:intron 65.0%
GGCCATGATCCGCAGGGGAA+CGG + chr6_4:26439834-26439853 Msa0983320:intron 65.0%
TTCATGAGACCGCGGCGGAG+TGG - chr6_4:26439991-26440010 None:intergenic 65.0%
TTCCCGCGCGCGAAGCGAAT+GGG + chr6_4:26440026-26440045 Msa0983320:CDS 65.0%
! AGCGAATGGGCGCTGTGCTG+TGG + chr6_4:26440039-26440058 Msa0983320:CDS 65.0%
! GCGAATGGGCGCTGTGCTGT+GGG + chr6_4:26440040-26440059 Msa0983320:CDS 65.0%
GCTGTGGGTCAGTTTCGGGG+CGG + chr6_4:26440055-26440074 Msa0983320:CDS 65.0%
CTGTGGGTCAGTTTCGGGGC+GGG + chr6_4:26440056-26440075 Msa0983320:CDS 65.0%
TGTGGGTCAGTTTCGGGGCG+GGG + chr6_4:26440057-26440076 Msa0983320:CDS 65.0%
GGGACGATGCCGGGGAGAGA+AGG - chr6_4:26439599-26439618 None:intergenic 70.0%
CCCGGCCCGACCTGTAGTGT+AGG + chr6_4:26439643-26439662 Msa0983320:intron 70.0%
TTTGCGCTGCGCGTTCCCGC+TGG - chr6_4:26439759-26439778 None:intergenic 70.0%
CCGATGGCCTGCGGGGGATT+CGG - chr6_4:26439800-26439819 None:intergenic 70.0%
CCGAATCCCCCGCAGGCCAT+CGG + chr6_4:26439797-26439816 Msa0983320:CDS 70.0%
CGAATCCCCCGCAGGCCATC+GGG + chr6_4:26439798-26439817 Msa0983320:CDS 70.0%
GAATCCCCCGCAGGCCATCG+GGG + chr6_4:26439799-26439818 Msa0983320:CDS 70.0%
ACTCGGTCCCCGATGGCCTG+CGG - chr6_4:26439809-26439828 None:intergenic 70.0%
CATTCGCTTCGCGCGCGGGA+AGG - chr6_4:26440027-26440046 None:intergenic 70.0%
CTTCCCGCGCGCGAAGCGAA+TGG + chr6_4:26440025-26440044 Msa0983320:CDS 70.0%
GTGGGTCAGTTTCGGGGCGG+GGG + chr6_4:26440058-26440077 Msa0983320:CDS 70.0%
TGGGTCAGTTTCGGGGCGGG+GGG + chr6_4:26440059-26440078 Msa0983320:CDS 70.0%
GCTGCGCGTTCCCGCTGGAC+TGG - chr6_4:26439754-26439773 None:intergenic 75.0%
TCGGTCCCCGATGGCCTGCG+GGG - chr6_4:26439807-26439826 None:intergenic 75.0%
CTCGGTCCCCGATGGCCTGC+GGG - chr6_4:26439808-26439827 None:intergenic 75.0%
GCAGGCCATCGGGGACCGAG+TGG + chr6_4:26439808-26439827 Msa0983320:CDS 75.0%
CGCCCATTCGCTTCGCGCGC+GGG - chr6_4:26440031-26440050 None:intergenic 75.0%
GCGCCCATTCGCTTCGCGCG+CGG - chr6_4:26440032-26440051 None:intergenic 75.0%
! CGGTCCCCGATGGCCTGCGG+GGG - chr6_4:26439806-26439825 None:intergenic 80.0%
! GCGGCTGCTCCACTCCGCCG+CGG + chr6_4:26439979-26439998 Msa0983320:CDS 80.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 26439452 26440103 26439452 ID=Msa0983320;Name=Msa0983320
chr6_4 mRNA 26439452 26440103 26439452 ID=Msa0983320-mRNA-1;Parent=Msa0983320;Name=Msa0983320-mRNA-1;_AED=0.46;_eAED=0.50;_QI=0|0|0|1|0|0.33|3|0|175
chr6_4 exon 26439452 26439613 26439452 ID=Msa0983320-mRNA-1:exon:7440;Parent=Msa0983320-mRNA-1
chr6_4 exon 26439685 26439829 26439685 ID=Msa0983320-mRNA-1:exon:7441;Parent=Msa0983320-mRNA-1
chr6_4 exon 26439883 26440103 26439883 ID=Msa0983320-mRNA-1:exon:7442;Parent=Msa0983320-mRNA-1
chr6_4 CDS 26439452 26439613 26439452 ID=Msa0983320-mRNA-1:cds;Parent=Msa0983320-mRNA-1
chr6_4 CDS 26439685 26439829 26439685 ID=Msa0983320-mRNA-1:cds;Parent=Msa0983320-mRNA-1
chr6_4 CDS 26439883 26440103 26439883 ID=Msa0983320-mRNA-1:cds;Parent=Msa0983320-mRNA-1
Gene Sequence

>Msa0983320

ATGTATCTGATGGAGTCTACATCGTACGTAGAGCGCCCAAGCGCCAGCTCAGTTCTCTTATCCATCGGTCCAATGCACTGTCATGGATGGAGGGAAAAGCAAAAATCTAGTTGTGTTGTTGTTGTTCGCCGCCTCGACGCATTCCCTTCTCTCCCCGGCATCGAGTTGAGCCTGAACTGGCGAACCGAAGTCACTTTCGGAACCATACTTCCTACAGCTAACACGTGTCCAGTCCAGCGGGAACGCGCAGCGCAAAAAAACGTAAGCTGCTATACCGAATCCCCCGCAGGCCATCGGGGACCGAGTGGTGCACGAACGACAACTCCAAACGTCACACATCCGTCGCCTACTTACCGTTTAGGTGGCACCAGCGAGATCCAGCTAAGGGAAGGAACTTCGTGTCGCGGCTGCTCCACTCCGCCGCGGTCTCATGAACCGAACTTCGTAGCCTTCCCGCGCGCGAAGCGAATGGGCGCTGTGCTGTGGGTCAGTTTCGGGGCGGGGGGCGAAGAGAGACCACAAGAATGA

Protein sequence

>Msa0983320

MYLMESTSYVERPSASSVLLSIGPMHCHGWREKQKSSCVVVVRRLDAFPSLPGIELSLNWRTEVTFGTILPTANTCPVQRERAAQKNVSCYTESPAGHRGPSGARTTTPNVTHPSPTYRLGGTSEIQLREGTSCRGCSTPPRSHEPNFVAFPRAKRMGAVLWVSFGAGGEERPQE*