Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983310 | RYR03716.1 | 86.667 | 45 | 6 | 0 | 1 | 45 | 1 | 45 | 8.20e-18 | 81.6 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0983310 | A0A444YP21 | 86.667 | 45 | 6 | 0 | 1 | 45 | 1 | 45 | 3.92e-18 | 81.6 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0983190 | Msa0983310 | 0.835573 | 1.551209e-56 | -8.615850e-47 |
| Msa0983300 | Msa0983310 | 0.803693 | 3.085722e-49 | -8.615850e-47 |
| Msa0983310 | Msa0983320 | 0.838828 | 2.281005e-57 | -8.615850e-47 |
| Msa0983310 | Msa0983350 | 0.810648 | 1.038496e-50 | -8.615850e-47 |
| Msa0983310 | Msa0983460 | 0.822041 | 2.928957e-53 | -8.615850e-47 |
| Msa0983310 | Msa0983500 | 0.806730 | 7.136987e-50 | -8.615850e-47 |
| Msa0983310 | Msa0983520 | 0.833495 | 5.161435e-56 | -8.615850e-47 |
| Msa0983310 | Msa1391970 | 0.811204 | 7.869118e-51 | -8.615850e-47 |
| Msa0983310 | Msa1392010 | 0.835151 | 1.983202e-56 | -8.615850e-47 |
| Msa0983310 | Msa1392020 | 0.972837 | 2.018827e-135 | -8.615850e-47 |
| Msa0983310 | Msa1392030 | 0.804122 | 2.512998e-49 | -8.615850e-47 |
| Msa0983310 | Msa1392120 | 0.814065 | 1.862918e-51 | -8.615850e-47 |
| Msa0983310 | Msa1392160 | 0.820871 | 5.453923e-53 | -8.615850e-47 |
| Msa0908850 | Msa0983310 | 0.831309 | 1.795639e-55 | -8.615850e-47 |
| Msa0944900 | Msa0983310 | 0.818009 | 2.451052e-52 | -8.615850e-47 |
| Msa0944920 | Msa0983310 | 0.837467 | 5.109840e-57 | -8.615850e-47 |
| Msa0944990 | Msa0983310 | 0.802270 | 6.075997e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCGCCCCGGGACGGGAGT+GGG | 0.361835 | 6_4:+26439076 | Msa0983310:CDS |
| CGCGCAGGCAACAGCAGCAC+CGG | 0.381366 | 6_4:+26439109 | Msa0983310:CDS |
| ACTCCCGTCCCGGGGCGCTA+AGG | 0.393401 | 6_4:-26439073 | None:intergenic |
| TATTTATTGTGGCTATATGT+TGG | 0.425913 | 6_4:+26439240 | Msa0983310:CDS |
| AGCGCCCCGGGACGGGAGTG+GGG | 0.428726 | 6_4:+26439077 | Msa0983310:CDS |
| CTGCTTCTTCTTATTTATTG+TGG | 0.439184 | 6_4:+26439229 | Msa0983310:CDS |
| GCTGCTGTTGCCTGCGCGTA+GGG | 0.444552 | 6_4:-26439104 | None:intergenic |
| CGCAACAAAAGCTTGATTCA+AGG | 0.460458 | 6_4:+26439010 | Msa0983310:CDS |
| CAAAAGCCCCTTAGCGCCCC+GGG | 0.475074 | 6_4:+26439065 | Msa0983310:CDS |
| CACAATAAATAAGAAGAAGC+AGG | 0.506204 | 6_4:-26439228 | None:intergenic |
| TGCTGCTGTTGCCTGCGCGT+AGG | 0.513689 | 6_4:-26439105 | None:intergenic |
| ACAAAAGCCCCTTAGCGCCC+CGG | 0.520929 | 6_4:+26439064 | Msa0983310:CDS |
| AGATTCTGACTTCGTAGAGC+CGG | 0.543209 | 6_4:-26439128 | None:intergenic |
| ATTGTGGCTATATGTTGGCG+TGG | 0.693941 | 6_4:+26439245 | Msa0983310:CDS |
| GTGGCTATATGTTGGCGTGG+CGG | 0.707120 | 6_4:+26439248 | Msa0983310:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCTTATTCACGAATGAATT+GGG | - | chr6_4:26439172-26439191 | None:intergenic | 25.0% |
| ! | ATTCTTATTCACGAATGAAT+TGG | - | chr6_4:26439173-26439192 | None:intergenic | 25.0% |
| ! | ATTCGTGAATAAGAATTCAA+GGG | + | chr6_4:26439177-26439196 | Msa0983310:intron | 25.0% |
| ! | TATTTATTGTGGCTATATGT+TGG | + | chr6_4:26439240-26439259 | Msa0983310:CDS | 25.0% |
| TCAGAATCTAATCTTTGTGT+TGG | + | chr6_4:26439142-26439161 | Msa0983310:intron | 30.0% | |
| CATTCGTGAATAAGAATTCA+AGG | + | chr6_4:26439176-26439195 | Msa0983310:intron | 30.0% | |
| CACAATAAATAAGAAGAAGC+AGG | - | chr6_4:26439231-26439250 | None:intergenic | 30.0% | |
| ! | CTGCTTCTTCTTATTTATTG+TGG | + | chr6_4:26439229-26439248 | Msa0983310:CDS | 30.0% |
| !!! | GCTTTTGTTTTTGGAACAGA+CGG | - | chr6_4:26439052-26439071 | None:intergenic | 35.0% |
| CGCAACAAAAGCTTGATTCA+AGG | + | chr6_4:26439010-26439029 | Msa0983310:CDS | 40.0% | |
| !!! | CGCTAAGGGGCTTTTGTTTT+TGG | - | chr6_4:26439061-26439080 | None:intergenic | 45.0% |
| AGATTCTGACTTCGTAGAGC+CGG | - | chr6_4:26439131-26439150 | None:intergenic | 45.0% | |
| ATTGTGGCTATATGTTGGCG+TGG | + | chr6_4:26439245-26439264 | Msa0983310:CDS | 45.0% | |
| GTGGCTATATGTTGGCGTGG+CGG | + | chr6_4:26439248-26439267 | Msa0983310:CDS | 55.0% | |
| ACAAAAGCCCCTTAGCGCCC+CGG | + | chr6_4:26439064-26439083 | Msa0983310:CDS | 60.0% | |
| CAAAAGCCCCTTAGCGCCCC+GGG | + | chr6_4:26439065-26439084 | Msa0983310:CDS | 65.0% | |
| GCTGCTGTTGCCTGCGCGTA+GGG | - | chr6_4:26439107-26439126 | None:intergenic | 65.0% | |
| TGCTGCTGTTGCCTGCGCGT+AGG | - | chr6_4:26439108-26439127 | None:intergenic | 65.0% | |
| CGCGCAGGCAACAGCAGCAC+CGG | + | chr6_4:26439109-26439128 | Msa0983310:CDS | 70.0% | |
| AGCCCCTTAGCGCCCCGGGA+CGG | + | chr6_4:26439069-26439088 | Msa0983310:CDS | 75.0% | |
| ! | TCCCGTCCCGGGGCGCTAAG+GGG | - | chr6_4:26439074-26439093 | None:intergenic | 75.0% |
| ! | CTCCCGTCCCGGGGCGCTAA+GGG | - | chr6_4:26439075-26439094 | None:intergenic | 75.0% |
| ! | ACTCCCGTCCCGGGGCGCTA+AGG | - | chr6_4:26439076-26439095 | None:intergenic | 75.0% |
| TTAGCGCCCCGGGACGGGAG+TGG | + | chr6_4:26439075-26439094 | Msa0983310:CDS | 75.0% | |
| TAGCGCCCCGGGACGGGAGT+GGG | + | chr6_4:26439076-26439095 | Msa0983310:CDS | 75.0% | |
| TGTCCCCCACTCCCGTCCCG+GGG | - | chr6_4:26439084-26439103 | None:intergenic | 75.0% | |
| GTGTCCCCCACTCCCGTCCC+GGG | - | chr6_4:26439085-26439104 | None:intergenic | 75.0% | |
| GGTGTCCCCCACTCCCGTCC+CGG | - | chr6_4:26439086-26439105 | None:intergenic | 75.0% | |
| GTGGGGGACACCCTACGCGC+AGG | + | chr6_4:26439094-26439113 | Msa0983310:CDS | 75.0% | |
| ! | GCCCCTTAGCGCCCCGGGAC+GGG | + | chr6_4:26439070-26439089 | Msa0983310:CDS | 80.0% |
| ! | AGCGCCCCGGGACGGGAGTG+GGG | + | chr6_4:26439077-26439096 | Msa0983310:CDS | 80.0% |
| ! | GCGCCCCGGGACGGGAGTGG+GGG | + | chr6_4:26439078-26439097 | Msa0983310:CDS | 85.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 26439006 | 26439305 | 26439006 | ID=Msa0983310;Name=Msa0983310 |
| chr6_4 | mRNA | 26439006 | 26439305 | 26439006 | ID=Msa0983310-mRNA-1;Parent=Msa0983310;Name=Msa0983310-mRNA-1;_AED=0.85;_eAED=1.00;_QI=0|0|0|0.5|0|0|2|0|78 |
| chr6_4 | exon | 26439006 | 26439140 | 26439006 | ID=Msa0983310-mRNA-1:exon:7488;Parent=Msa0983310-mRNA-1 |
| chr6_4 | exon | 26439204 | 26439305 | 26439204 | ID=Msa0983310-mRNA-1:exon:7489;Parent=Msa0983310-mRNA-1 |
| chr6_4 | CDS | 26439006 | 26439140 | 26439006 | ID=Msa0983310-mRNA-1:cds;Parent=Msa0983310-mRNA-1 |
| chr6_4 | CDS | 26439204 | 26439305 | 26439204 | ID=Msa0983310-mRNA-1:cds;Parent=Msa0983310-mRNA-1 |
| Gene Sequence |
| Protein sequence |
