AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1392010


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 68 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTGCTGTGGGTCAGTTTC+GGG 0.191312 tig0021468:-44620 Msa1392010:CDS
TGTGGGTCAGTTTCGGGGCG+GGG 0.313334 tig0021468:-44614 Msa1392010:CDS
CTTTCTGTGTGGGACGATGC+CGG 0.316270 tig0021468:+45065 None:intergenic
GCTGTGCTGTGGGTCAGTTT+CGG 0.325201 tig0021468:-44621 Msa1392010:CDS
GTGGGTCAGTTTCGGGGCGG+GGG 0.330602 tig0021468:-44613 Msa1392010:CDS
CTGTGGGTCAGTTTCGGGGC+GGG 0.351980 tig0021468:-44615 Msa1392010:CDS
CGCCCATTCGCTTCGCGCGC+GGG 0.358241 tig0021468:+44643 None:intergenic
TGACTTCGGTTCGCCAGTTC+AGG 0.360274 tig0021468:+44978 None:intergenic
TTCCCGCGCGCGAAGCGAAT+GGG 0.362487 tig0021468:-44645 Msa1392010:CDS
TTTGCGCTGCGCGTTCCCGC+TGG 0.368645 tig0021468:+44915 None:intergenic
TTTCTGTGTGGGACGATGCC+GGG 0.378127 tig0021468:+45066 None:intergenic
CGCGGGAAGGCTACGAAGTT+CGG 0.381304 tig0021468:+44660 None:intergenic
CTCGGTCCCCGATGGCCTGC+GGG 0.387846 tig0021468:+44866 None:intergenic
CTTCCCGCGCGCGAAGCGAA+TGG 0.392844 tig0021468:-44646 Msa1392010:CDS
CCGTCGCCTACTTACCGTTT+AGG 0.396921 tig0021468:-44756 Msa1392010:CDS
ACGAAGTTCCTTCCCTTAGC+TGG 0.398339 tig0021468:+44718 None:intergenic
TGGCGAACCGAAGTCACTTT+CGG 0.415405 tig0021468:-44971 Msa1392010:CDS
GCGCCCATTCGCTTCGCGCG+CGG 0.425723 tig0021468:+44642 None:intergenic
AGCGAATGGGCGCTGTGCTG+TGG 0.429564 tig0021468:-44632 Msa1392010:CDS
CATTCGCTTCGCGCGCGGGA+AGG 0.433191 tig0021468:+44647 None:intergenic
TGGGTCAGTTTCGGGGCGGG+GGG 0.448710 tig0021468:-44612 Msa1392010:CDS
GCTGCGCGTTCCCGCTGGAC+TGG 0.454225 tig0021468:+44920 None:intergenic
AAGAGAACTGAGCTGGCGCT+TGG 0.459565 tig0021468:+45183 None:intergenic
CGTGTTAGCTGTAGGAAGTA+TGG 0.472037 tig0021468:+44946 None:intergenic
GCGAGGAGTTGAGCCTGAAC+TGG 0.476860 tig0021468:-44991 Msa1392010:intron
GGGACGATGCCGGGGAGAGA+AGG 0.483121 tig0021468:+45075 None:intergenic
TCTCGCTGGTGCCACCTAAA+CGG 0.485900 tig0021468:+44742 None:intergenic
TTCCCTTAGCTGGATCTCGC+TGG 0.493334 tig0021468:+44728 None:intergenic
AGAGAACTGAGCTGGCGCTT+GGG 0.494487 tig0021468:+45184 None:intergenic
GGACGATGCCGGGGAGAGAA+GGG 0.500282 tig0021468:+45076 None:intergenic
GGCGACGGATGTGTGACGTT+TGG 0.502967 tig0021468:+44771 None:intergenic
GACGCATTCCCTTCTCTCCC+CGG 0.520898 tig0021468:-45084 Msa1392010:CDS
ATAAATCGATATGTATCTGA+TGG 0.523902 tig0021468:-45229 None:intergenic
CTCCATCCATGACAGTGCAT+TGG 0.532650 tig0021468:+45150 None:intergenic
GTCCAATGCACTGTCATGGA+TGG 0.536192 tig0021468:-45152 Msa1392010:CDS
GCGAATGGGCGCTGTGCTGT+GGG 0.539770 tig0021468:-44631 Msa1392010:CDS
AGCTCAGTTCTCTTATCCAT+CGG 0.540408 tig0021468:-45174 Msa1392010:CDS
CAATGCACTGTCATGGATGG+AGG 0.543873 tig0021468:-45149 Msa1392010:CDS
TTCATGAGACCGCGGCGGAG+TGG 0.545398 tig0021468:+44683 None:intergenic
ACTCGGTCCCCGATGGCCTG+CGG 0.553396 tig0021468:+44865 None:intergenic
ATCGGTCCAATGCACTGTCA+TGG 0.554798 tig0021468:-45156 Msa1392010:CDS
GCTGTGGGTCAGTTTCGGGG+CGG 0.555599 tig0021468:-44616 Msa1392010:CDS
GATGGATAAGAGAACTGAGC+TGG 0.556532 tig0021468:+45176 None:intergenic
ATGACAGTGCATTGGACCGA+TGG 0.558632 tig0021468:+45158 None:intergenic
TTCTGTGTGGGACGATGCCG+GGG 0.559319 tig0021468:+45067 None:intergenic
CCTTACCACTCGGTCCCCGA+TGG 0.572919 tig0021468:+44858 None:intergenic
TCGGTCCCCGATGGCCTGCG+GGG 0.576019 tig0021468:+44867 None:intergenic
TATGGTTCCGAAAGTGACTT+CGG 0.579844 tig0021468:+44964 None:intergenic
ATCCAAATGAATCATTCTTG+TGG 0.586529 tig0021468:+44579 None:intergenic
CGGTCCCCGATGGCCTGCGG+GGG 0.603794 tig0021468:+44868 None:intergenic
TAACACGTGTCCAGTCCAGC+GGG 0.606235 tig0021468:-44930 Msa1392010:CDS
GTGCCACCTAAACGGTAAGT+AGG 0.609035 tig0021468:+44750 None:intergenic
TGTGCTGTGGGTCAGTTTCG+GGG 0.610535 tig0021468:-44619 Msa1392010:CDS
AGCGAGATCCAGCTAAGGGA+AGG 0.613371 tig0021468:-44726 Msa1392010:CDS
GCACCAGCGAGATCCAGCTA+AGG 0.613771 tig0021468:-44731 Msa1392010:CDS
CACCAGCGAGATCCAGCTAA+GGG 0.620544 tig0021468:-44730 Msa1392010:CDS
AATGCACTGTCATGGATGGA+GGG 0.629806 tig0021468:-45148 Msa1392010:CDS
ACTGGACACGTGTTAGCTGT+AGG 0.636729 tig0021468:+44938 None:intergenic
TCGCCTACTTACCGTTTAGG+TGG 0.640918 tig0021468:-44753 Msa1392010:CDS
GCGGCTGCTCCACTCCGCCG+CGG 0.642559 tig0021468:-44692 Msa1392010:CDS
GGAGAGAAGGGAATGCGTCG+AGG 0.646607 tig0021468:+45088 None:intergenic
GAGAAGGGAATGCGTCGAGG+CGG 0.663253 tig0021468:+45091 None:intergenic
GCAGGCCATCGGGGACCGAG+TGG 0.669923 tig0021468:-44863 Msa1392010:intron
CTAACACGTGTCCAGTCCAG+CGG 0.686803 tig0021468:-44931 Msa1392010:CDS
AGGGAAGGAACTTCGTGTCG+CGG 0.689775 tig0021468:-44711 Msa1392010:CDS
CCTAAACGGTAAGTAGGCGA+CGG 0.699145 tig0021468:+44756 None:intergenic
TTCGGTTCATGAGACCGCGG+CGG 0.744018 tig0021468:+44678 None:intergenic
AAGTTCGGTTCATGAGACCG+CGG 0.751147 tig0021468:+44675 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGCTCAGTTCTCTTATCCAT+CGG - tig0021468:44635-44654 Msa1392010:CDS 40.0%
TATGGTTCCGAAAGTGACTT+CGG + tig0021468:44848-44867 None:intergenic 40.0%
GGATCACTCATTCTTCCATT+GGG - tig0021468:44993-45012 Msa1392010:intron 40.0%
GATCACTCATTCTTCCATTG+GGG - tig0021468:44994-45013 Msa1392010:intron 40.0%
GATGGATAAGAGAACTGAGC+TGG + tig0021468:44636-44655 None:intergenic 45.0%
AATGCACTGTCATGGATGGA+GGG - tig0021468:44661-44680 Msa1392010:CDS 45.0%
TGCGCTCTTTCTTTCTGTGT+GGG + tig0021468:44757-44776 None:intergenic 45.0%
AACTCCTCGCTTTACGTTAG+CGG + tig0021468:44808-44827 None:intergenic 45.0%
CGTGTTAGCTGTAGGAAGTA+TGG + tig0021468:44866-44885 None:intergenic 45.0%
CGGATCACTCATTCTTCCAT+TGG - tig0021468:44992-45011 Msa1392010:intron 45.0%
ATGACAGTGCATTGGACCGA+TGG + tig0021468:44654-44673 None:intergenic 50.0%
ATCGGTCCAATGCACTGTCA+TGG - tig0021468:44653-44672 Msa1392010:CDS 50.0%
GTCCAATGCACTGTCATGGA+TGG - tig0021468:44657-44676 Msa1392010:CDS 50.0%
CTCCATCCATGACAGTGCAT+TGG + tig0021468:44662-44681 None:intergenic 50.0%
CAATGCACTGTCATGGATGG+AGG - tig0021468:44660-44679 Msa1392010:CDS 50.0%
CTGCGCTCTTTCTTTCTGTG+TGG + tig0021468:44758-44777 None:intergenic 50.0%
! TGGCGAACCGAAGTCACTTT+CGG - tig0021468:44838-44857 Msa1392010:intron 50.0%
ACTGGACACGTGTTAGCTGT+AGG + tig0021468:44874-44893 None:intergenic 50.0%
CTCATTCTTCCATTGGGGAC+AGG - tig0021468:44999-45018 Msa1392010:intron 50.0%
CCTAAACGGTAAGTAGGCGA+CGG + tig0021468:45056-45075 None:intergenic 50.0%
TCGCCTACTTACCGTTTAGG+TGG - tig0021468:45056-45075 Msa1392010:intron 50.0%
GTGCCACCTAAACGGTAAGT+AGG + tig0021468:45062-45081 None:intergenic 50.0%
ACGAAGTTCCTTCCCTTAGC+TGG + tig0021468:45094-45113 None:intergenic 50.0%
AAGTTCGGTTCATGAGACCG+CGG + tig0021468:45137-45156 None:intergenic 50.0%
AGAGAACTGAGCTGGCGCTT+GGG + tig0021468:44628-44647 None:intergenic 55.0%
AAGAGAACTGAGCTGGCGCT+TGG + tig0021468:44629-44648 None:intergenic 55.0%
TTTCTGTGTGGGACGATGCC+GGG + tig0021468:44746-44765 None:intergenic 55.0%
CTTTCTGTGTGGGACGATGC+CGG + tig0021468:44747-44766 None:intergenic 55.0%
! AAAGAAAGAGCGCAGAGCCC+CGG - tig0021468:44763-44782 Msa1392010:CDS 55.0%
AGCGGACCTACACTACAGGT+CGG + tig0021468:44790-44809 None:intergenic 55.0%
! CGTTAGCGGACCTACACTAC+AGG + tig0021468:44794-44813 None:intergenic 55.0%
AGGTCCGCTAACGTAAAGCG+AGG - tig0021468:44801-44820 Msa1392010:intron 55.0%
! TGACTTCGGTTCGCCAGTTC+AGG + tig0021468:44834-44853 None:intergenic 55.0%
CTAACACGTGTCCAGTCCAG+CGG - tig0021468:44878-44897 Msa1392010:CDS 55.0%
TAACACGTGTCCAGTCCAGC+GGG - tig0021468:44879-44898 Msa1392010:CDS 55.0%
! CGGATCATGGCCTTACCACT+CGG + tig0021468:44964-44983 None:intergenic 55.0%
TGGTAAGGCCATGATCCGCA+GGG - tig0021468:44966-44985 Msa1392010:CDS 55.0%
ATGAGTGATCCGTTCCCCTG+CGG + tig0021468:44984-45003 None:intergenic 55.0%
CCGTCGCCTACTTACCGTTT+AGG - tig0021468:45053-45072 Msa1392010:intron 55.0%
!! TCTCGCTGGTGCCACCTAAA+CGG + tig0021468:45070-45089 None:intergenic 55.0%
CACCAGCGAGATCCAGCTAA+GGG - tig0021468:45079-45098 Msa1392010:intron 55.0%
TTCCCTTAGCTGGATCTCGC+TGG + tig0021468:45084-45103 None:intergenic 55.0%
AGCGAGATCCAGCTAAGGGA+AGG - tig0021468:45083-45102 Msa1392010:CDS 55.0%
AGGGAAGGAACTTCGTGTCG+CGG - tig0021468:45098-45117 Msa1392010:CDS 55.0%
GCTGTGCTGTGGGTCAGTTT+CGG - tig0021468:45188-45207 Msa1392010:CDS 55.0%
CTGTGCTGTGGGTCAGTTTC+GGG - tig0021468:45189-45208 Msa1392010:CDS 55.0%
TGTGCTGTGGGTCAGTTTCG+GGG - tig0021468:45190-45209 Msa1392010:CDS 55.0%
GAGAAGGGAATGCGTCGAGG+CGG + tig0021468:44721-44740 None:intergenic 60.0%
GGAGAGAAGGGAATGCGTCG+AGG + tig0021468:44724-44743 None:intergenic 60.0%
GACGCATTCCCTTCTCTCCC+CGG - tig0021468:44725-44744 Msa1392010:CDS 60.0%
TTCTGTGTGGGACGATGCCG+GGG + tig0021468:44745-44764 None:intergenic 60.0%
ACCTACACTACAGGTCGGGC+CGG + tig0021468:44785-44804 None:intergenic 60.0%
GCGGACCTACACTACAGGTC+GGG + tig0021468:44789-44808 None:intergenic 60.0%
GCGAGGAGTTGAGCCTGAAC+TGG - tig0021468:44818-44837 Msa1392010:intron 60.0%
TGCTATACCGAATCCCCCGC+AGG - tig0021468:44928-44947 Msa1392010:CDS 60.0%
GTGGTAAGGCCATGATCCGC+AGG - tig0021468:44965-44984 Msa1392010:CDS 60.0%
GGTAAGGCCATGATCCGCAG+GGG - tig0021468:44967-44986 Msa1392010:CDS 60.0%
ATCCGTTCCCCTGCGGATCA+TGG + tig0021468:44977-44996 None:intergenic 60.0%
GTTCGTGCACCTGTCCCCAA+TGG + tig0021468:45011-45030 None:intergenic 60.0%
GGCGACGGATGTGTGACGTT+TGG + tig0021468:45041-45060 None:intergenic 60.0%
GCACCAGCGAGATCCAGCTA+AGG - tig0021468:45078-45097 Msa1392010:intron 60.0%
TTCGGTTCATGAGACCGCGG+CGG + tig0021468:45134-45153 None:intergenic 60.0%
CGCGGGAAGGCTACGAAGTT+CGG + tig0021468:45152-45171 None:intergenic 60.0%
GGACGATGCCGGGGAGAGAA+GGG + tig0021468:44736-44755 None:intergenic 65.0%
CTACACTACAGGTCGGGCCG+GGG + tig0021468:44783-44802 None:intergenic 65.0%
CCTACACTACAGGTCGGGCC+GGG + tig0021468:44784-44803 None:intergenic 65.0%
CCTTACCACTCGGTCCCCGA+TGG + tig0021468:44954-44973 None:intergenic 65.0%
CCATCGGGGACCGAGTGGTA+AGG - tig0021468:44951-44970 Msa1392010:CDS 65.0%
GGCCATGATCCGCAGGGGAA+CGG - tig0021468:44972-44991 Msa1392010:CDS 65.0%
TTCATGAGACCGCGGCGGAG+TGG + tig0021468:45129-45148 None:intergenic 65.0%
TTCCCGCGCGCGAAGCGAAT+GGG - tig0021468:45164-45183 Msa1392010:CDS 65.0%
! AGCGAATGGGCGCTGTGCTG+TGG - tig0021468:45177-45196 Msa1392010:CDS 65.0%
! GCGAATGGGCGCTGTGCTGT+GGG - tig0021468:45178-45197 Msa1392010:CDS 65.0%
GCTGTGGGTCAGTTTCGGGG+CGG - tig0021468:45193-45212 Msa1392010:CDS 65.0%
CTGTGGGTCAGTTTCGGGGC+GGG - tig0021468:45194-45213 Msa1392010:CDS 65.0%
TGTGGGTCAGTTTCGGGGCG+GGG - tig0021468:45195-45214 Msa1392010:CDS 65.0%
GGGACGATGCCGGGGAGAGA+AGG + tig0021468:44737-44756 None:intergenic 70.0%
CCCGGCCCGACCTGTAGTGT+AGG - tig0021468:44781-44800 Msa1392010:CDS 70.0%
TTTGCGCTGCGCGTTCCCGC+TGG + tig0021468:44897-44916 None:intergenic 70.0%
CCGATGGCCTGCGGGGGATT+CGG + tig0021468:44938-44957 None:intergenic 70.0%
CCGAATCCCCCGCAGGCCAT+CGG - tig0021468:44935-44954 Msa1392010:CDS 70.0%
CGAATCCCCCGCAGGCCATC+GGG - tig0021468:44936-44955 Msa1392010:CDS 70.0%
GAATCCCCCGCAGGCCATCG+GGG - tig0021468:44937-44956 Msa1392010:CDS 70.0%
ACTCGGTCCCCGATGGCCTG+CGG + tig0021468:44947-44966 None:intergenic 70.0%
CATTCGCTTCGCGCGCGGGA+AGG + tig0021468:45165-45184 None:intergenic 70.0%
CTTCCCGCGCGCGAAGCGAA+TGG - tig0021468:45163-45182 Msa1392010:CDS 70.0%
GTGGGTCAGTTTCGGGGCGG+GGG - tig0021468:45196-45215 Msa1392010:CDS 70.0%
TGGGTCAGTTTCGGGGCGGG+GGG - tig0021468:45197-45216 Msa1392010:CDS 70.0%
GCTGCGCGTTCCCGCTGGAC+TGG + tig0021468:44892-44911 None:intergenic 75.0%
TCGGTCCCCGATGGCCTGCG+GGG + tig0021468:44945-44964 None:intergenic 75.0%
CTCGGTCCCCGATGGCCTGC+GGG + tig0021468:44946-44965 None:intergenic 75.0%
GCAGGCCATCGGGGACCGAG+TGG - tig0021468:44946-44965 Msa1392010:CDS 75.0%
CGCCCATTCGCTTCGCGCGC+GGG + tig0021468:45169-45188 None:intergenic 75.0%
GCGCCCATTCGCTTCGCGCG+CGG + tig0021468:45170-45189 None:intergenic 75.0%
! CGGTCCCCGATGGCCTGCGG+GGG + tig0021468:44944-44963 None:intergenic 80.0%
! GCGGCTGCTCCACTCCGCCG+CGG - tig0021468:45117-45136 Msa1392010:CDS 80.0%
Chromosome Type Strat End Strand Name
tig0021468 gene 44590 45241 44590 ID=Msa1392010;Name=Msa1392010
tig0021468 mRNA 44590 45241 44590 ID=Msa1392010-mRNA-1;Parent=Msa1392010;Name=Msa1392010-mRNA-1;_AED=0.41;_eAED=0.46;_QI=0|0|0|1|0|0.33|3|0|175
tig0021468 exon 45080 45241 45080 ID=Msa1392010-mRNA-1:exon:12979;Parent=Msa1392010-mRNA-1
tig0021468 exon 44864 45008 44864 ID=Msa1392010-mRNA-1:exon:12978;Parent=Msa1392010-mRNA-1
tig0021468 exon 44590 44810 44590 ID=Msa1392010-mRNA-1:exon:12977;Parent=Msa1392010-mRNA-1
tig0021468 CDS 45080 45241 45080 ID=Msa1392010-mRNA-1:cds;Parent=Msa1392010-mRNA-1
tig0021468 CDS 44864 45008 44864 ID=Msa1392010-mRNA-1:cds;Parent=Msa1392010-mRNA-1
tig0021468 CDS 44590 44810 44590 ID=Msa1392010-mRNA-1:cds;Parent=Msa1392010-mRNA-1
Gene Sequence

>Msa1392010

ATGTATCTGATGGAGTCTACATCGTACGTAGAGCGCCCAAGCGCCAGCTCAGTTCTCTTATCCATCGGTCCAATGCACTGTCATGGATGGAGGGAAAAGCAAAAATCTAGTTGTGTTGTTGTTGTTCGCCGCCTCGACGCATTCCCTTCTCTCCCCGGCATCGAGTTGAGCCTGAACTGGCGAACCGAAGTCACTTTCGGAACCATACTTCCTACAGCTAACACGTGTCCAGTCCAGCGGGAACGCGCAGCGCAAAAAAACGTAAGCTGCTATACCGAATCCCCCGCAGGCCATCGGGGACCGAGTGGTGCACGAACGACAACTCCAAACGTCACACATCCGTCGCCTACTTACCGTTTAGGTGGCACCAGCGAGATCCAGCTAAGGGAAGGAACTTCGTGTCGCGGCTGCTCCACTCCGCCGCGGTCTCATGAACCGAACTTCGTAGCCTTCCCGCGCGCGAAGCGAATGGGCGCTGTGCTGTGGGTCAGTTTCGGGGCGGGGGGCGAAGAGAGACCACAAGAATGA

Protein sequence

>Msa1392010

MYLMESTSYVERPSASSVLLSIGPMHCHGWREKQKSSCVVVVRRLDAFPSLPGIELSLNWRTEVTFGTILPTANTCPVQRERAAQKNVSCYTESPAGHRGPSGARTTTPNVTHPSPTYRLGGTSEIQLREGTSCRGCSTPPRSHEPNFVAFPRAKRMGAVLWVSFGAGGEERPQE*