Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1400320 | A0A072VYP4 | 88.571 | 35 | 4 | 0 | 1 | 35 | 14 | 48 | 6.91e-11 | 65.1 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0012530 | Msa1400320 | 0.804277 | 2.333520e-49 | -8.615850e-47 |
| Msa1400320 | Msa1410210 | 0.804110 | 2.527886e-49 | -8.615850e-47 |
| Msa0657970 | Msa1400320 | 0.815295 | 9.946127e-52 | -8.615850e-47 |
| Msa0685750 | Msa1400320 | 0.814961 | 1.179708e-51 | -8.615850e-47 |
| Msa0688070 | Msa1400320 | 0.803491 | 3.399390e-49 | -8.615850e-47 |
| Msa0123300 | Msa1400320 | 0.815264 | 1.010486e-51 | -8.615850e-47 |
| Msa0128120 | Msa1400320 | 0.807458 | 5.004303e-50 | -8.615850e-47 |
| Msa0166870 | Msa1400320 | 0.803241 | 3.828793e-49 | -8.615850e-47 |
| Msa1283860 | Msa1400320 | 0.814861 | 1.241912e-51 | -8.615850e-47 |
| Msa1306850 | Msa1400320 | 0.800627 | 1.319055e-48 | -8.615850e-47 |
| Msa1082500 | Msa1400320 | 0.838196 | 3.320526e-57 | -8.615850e-47 |
| Msa0285450 | Msa1400320 | 0.801839 | 7.450428e-49 | -8.615850e-47 |
| Msa1220150 | Msa1400320 | 0.804586 | 2.011996e-49 | -8.615850e-47 |
| Msa1224480 | Msa1400320 | 0.807145 | 5.832933e-50 | -8.615850e-47 |
| Msa0484780 | Msa1400320 | 0.822150 | 2.763480e-53 | -8.615850e-47 |
| Msa0512900 | Msa1400320 | 0.813298 | 2.746982e-51 | -8.615850e-47 |
| Msa0536220 | Msa1400320 | 0.800102 | 1.687218e-48 | -8.615850e-47 |
| Msa0954920 | Msa1400320 | 0.804894 | 1.735069e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGACAAACTTACCTTAA+TGG | 0.162835 | tig0022428:-101063 | None:intergenic |
| GCATCTTCCACTTTGGATTA+TGG | 0.189165 | tig0022428:-100553 | None:intergenic |
| CGAGAGAATTCAATCCATTA+AGG | 0.335586 | tig0022428:+101049 | Msa1400320:CDS |
| TCATGGCTAATTCTGGAAAC+TGG | 0.358644 | tig0022428:+101137 | Msa1400320:CDS |
| GAGATGACCCCACTAGAAAA+AGG | 0.372748 | tig0022428:+100529 | None:intergenic |
| TTGCAACTGATTTATCTTGT+TGG | 0.384750 | tig0022428:-101012 | None:intergenic |
| AAGGTTCCCACATCATCATT+TGG | 0.421371 | tig0022428:-100987 | None:intergenic |
| CAGCATACAAGAACAACAAC+AGG | 0.423932 | tig0022428:+100747 | Msa1400320:five_prime_UTR |
| CTTAATGGATTGAATTCTCT+CGG | 0.445675 | tig0022428:-101048 | None:intergenic |
| TTTAGAAATTGGGTTGGGTG+TGG | 0.466371 | tig0022428:-100578 | None:intergenic |
| AGAGTAGAGTAGAGTAGAGT+CGG | 0.466372 | tig0022428:-100675 | None:intergenic |
| TGGATTGAATTCTCTCGGCC+TGG | 0.469701 | tig0022428:-101043 | None:intergenic |
| TGTGGACGCATCTTCCACTT+TGG | 0.499394 | tig0022428:-100560 | None:intergenic |
| CTGATTTATCTTGTTGGACA+AGG | 0.531637 | tig0022428:-101006 | None:intergenic |
| ACAACAACAGGGAGCATCCA+TGG | 0.554285 | tig0022428:+100759 | Msa1400320:exon |
| TCAGTTGCAAAAGCAAATCC+AGG | 0.571291 | tig0022428:+101025 | Msa1400320:CDS |
| GAATCGTTGATCAACTCGCT+CGG | 0.579502 | tig0022428:+100722 | Msa1400320:five_prime_UTR |
| AGAGCCGCCAAATGATGATG+TGG | 0.582841 | tig0022428:+100980 | Msa1400320:CDS |
| GTTCCCACATCATCATTTGG+CGG | 0.610279 | tig0022428:-100984 | None:intergenic |
| AAAAGGTCCATAATCCAAAG+TGG | 0.613153 | tig0022428:+100546 | Msa1400320:five_prime_UTR |
| AACGTAACGTAACGTAACTG+TGG | 0.627244 | tig0022428:-100610 | None:intergenic |
| GAATTCAAGTGTGTGTAGCA+AGG | 0.628561 | tig0022428:-100644 | None:intergenic |
| GAGCCGCCAAATGATGATGT+GGG | 0.634443 | tig0022428:+100981 | Msa1400320:CDS |
| GTAACGTAACTGTGGACGCA+CGG | 0.642182 | tig0022428:-100602 | None:intergenic |
| AGCATACAAGAACAACAACA+GGG | 0.742063 | tig0022428:+100748 | Msa1400320:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATATTTTGTTTTGTAATAT+TGG | + | tig0022428:100952-100971 | Msa1400320:intron | 15.0% |
| !!! | TATAAGGTTTTGTGAAATTA+TGG | - | tig0022428:100888-100907 | None:intergenic | 20.0% |
| !! | AACGATCATATATAAAATTG+AGG | - | tig0022428:100925-100944 | None:intergenic | 20.0% |
| !!! | TTTTGTTTTGTAATATTGGC+AGG | + | tig0022428:100956-100975 | Msa1400320:intron | 25.0% |
| ! | AAATGACAAACTTACCTTAA+TGG | - | tig0022428:101066-101085 | None:intergenic | 25.0% |
| TAGAGTTGAATAGAGTGAAT+CGG | - | tig0022428:100788-100807 | None:intergenic | 30.0% | |
| ATGAAAGACTGTTTATGACA+GGG | - | tig0022428:100833-100852 | None:intergenic | 30.0% | |
| AATGAAAGACTGTTTATGAC+AGG | - | tig0022428:100834-100853 | None:intergenic | 30.0% | |
| GGTTAATTGAGTAGAGTATA+AGG | - | tig0022428:100904-100923 | None:intergenic | 30.0% | |
| ! | TTGCAACTGATTTATCTTGT+TGG | - | tig0022428:101015-101034 | None:intergenic | 30.0% |
| !! | CTTAATGGATTGAATTCTCT+CGG | - | tig0022428:101051-101070 | None:intergenic | 30.0% |
| !! | AATTTCTGTAGTTTGTTTGC+TGG | + | tig0022428:101095-101114 | Msa1400320:intron | 30.0% |
| !!! | GGATGTTCTTTGTTTTTTCA+TGG | + | tig0022428:101120-101139 | Msa1400320:intron | 30.0% |
| !!! | TGTTTTTTCATGGCTAATTC+TGG | + | tig0022428:101130-101149 | Msa1400320:CDS | 30.0% |
| AAAAGGTCCATAATCCAAAG+TGG | + | tig0022428:100546-100565 | Msa1400320:five_prime_UTR | 35.0% | |
| !!! | ACGGTTTTAGAAATTGGGTT+GGG | - | tig0022428:100586-100605 | None:intergenic | 35.0% |
| AGCATACAAGAACAACAACA+GGG | + | tig0022428:100748-100767 | Msa1400320:five_prime_UTR | 35.0% | |
| CTGATTTATCTTGTTGGACA+AGG | - | tig0022428:101009-101028 | None:intergenic | 35.0% | |
| CGAGAGAATTCAATCCATTA+AGG | + | tig0022428:101049-101068 | Msa1400320:CDS | 35.0% | |
| GCATCTTCCACTTTGGATTA+TGG | - | tig0022428:100556-100575 | None:intergenic | 40.0% | |
| !! | TTTAGAAATTGGGTTGGGTG+TGG | - | tig0022428:100581-100600 | None:intergenic | 40.0% |
| !!! | CACGGTTTTAGAAATTGGGT+TGG | - | tig0022428:100587-100606 | None:intergenic | 40.0% |
| !!! | GACGCACGGTTTTAGAAATT+GGG | - | tig0022428:100591-100610 | None:intergenic | 40.0% |
| AACGTAACGTAACGTAACTG+TGG | - | tig0022428:100613-100632 | None:intergenic | 40.0% | |
| GAATTCAAGTGTGTGTAGCA+AGG | - | tig0022428:100647-100666 | None:intergenic | 40.0% | |
| AGAGTAGAGTAGAGTAGAGT+CGG | - | tig0022428:100678-100697 | None:intergenic | 40.0% | |
| CAGCATACAAGAACAACAAC+AGG | + | tig0022428:100747-100766 | Msa1400320:five_prime_UTR | 40.0% | |
| ATAGAGTGAATCGGAAACCA+TGG | - | tig0022428:100779-100798 | None:intergenic | 40.0% | |
| TGTTTATGACAGGGATTGGA+AGG | - | tig0022428:100824-100843 | None:intergenic | 40.0% | |
| AGACTGTTTATGACAGGGAT+TGG | - | tig0022428:100828-100847 | None:intergenic | 40.0% | |
| AAGGTTCCCACATCATCATT+TGG | - | tig0022428:100990-101009 | None:intergenic | 40.0% | |
| TCAGTTGCAAAAGCAAATCC+AGG | + | tig0022428:101025-101044 | Msa1400320:CDS | 40.0% | |
| ! | TCATGGCTAATTCTGGAAAC+TGG | + | tig0022428:101137-101156 | Msa1400320:CDS | 40.0% |
| !!! | GGACGCACGGTTTTAGAAAT+TGG | - | tig0022428:100592-100611 | None:intergenic | 45.0% |
| GAATCGTTGATCAACTCGCT+CGG | + | tig0022428:100722-100741 | Msa1400320:five_prime_UTR | 45.0% | |
| GTTCCCACATCATCATTTGG+CGG | - | tig0022428:100987-101006 | None:intergenic | 45.0% | |
| !! | TCTGTAGTTTGTTTGCTGGC+AGG | + | tig0022428:101099-101118 | Msa1400320:intron | 45.0% |
| TGTGGACGCATCTTCCACTT+TGG | - | tig0022428:100563-100582 | None:intergenic | 50.0% | |
| GTAACGTAACTGTGGACGCA+CGG | - | tig0022428:100605-100624 | None:intergenic | 50.0% | |
| !! | ACAACAACAGGGAGCATCCA+TGG | + | tig0022428:100759-100778 | Msa1400320:exon | 50.0% |
| ! | AGAGCCGCCAAATGATGATG+TGG | + | tig0022428:100980-100999 | Msa1400320:CDS | 50.0% |
| ! | GAGCCGCCAAATGATGATGT+GGG | + | tig0022428:100981-101000 | Msa1400320:CDS | 50.0% |
| TGGATTGAATTCTCTCGGCC+TGG | - | tig0022428:101046-101065 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022428 | gene | 100544 | 101165 | 100544 | ID=Msa1400320;Name=Msa1400320 |
| tig0022428 | mRNA | 100544 | 101165 | 100544 | ID=Msa1400320-mRNA-1;Parent=Msa1400320;Name=Msa1400320-mRNA-1;_AED=0.28;_eAED=0.28;_QI=234|0.5|0.66|0.66|0|0.33|3|0|46 |
| tig0022428 | exon | 100544 | 100780 | 100544 | ID=Msa1400320-mRNA-1:exon:17663;Parent=Msa1400320-mRNA-1 |
| tig0022428 | exon | 100978 | 101070 | 100978 | ID=Msa1400320-mRNA-1:exon:17664;Parent=Msa1400320-mRNA-1 |
| tig0022428 | exon | 101121 | 101165 | 101121 | ID=Msa1400320-mRNA-1:exon:17665;Parent=Msa1400320-mRNA-1 |
| tig0022428 | five_prime_UTR | 100544 | 100777 | 100544 | ID=Msa1400320-mRNA-1:five_prime_utr;Parent=Msa1400320-mRNA-1 |
| tig0022428 | CDS | 100778 | 100780 | 100778 | ID=Msa1400320-mRNA-1:cds;Parent=Msa1400320-mRNA-1 |
| tig0022428 | CDS | 100978 | 101070 | 100978 | ID=Msa1400320-mRNA-1:cds;Parent=Msa1400320-mRNA-1 |
| tig0022428 | CDS | 101121 | 101165 | 101121 | ID=Msa1400320-mRNA-1:cds;Parent=Msa1400320-mRNA-1 |
| Gene Sequence |
| Protein sequence |
