AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1401100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401100 MtrunA17_Chr7g0274341 97.368 228 5 1 1 228 1 227 3.46e-157 434
Msa1401100 MtrunA17_Chr1g0190481 64.935 231 58 9 1 228 1 211 3.69e-67 206
Msa1401100 MtrunA17_Chr8g0346111 61.250 80 28 2 7 84 6 84 5.22e-25 97.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1401100 AT5G28640.1 57.265 234 70 5 1 228 1 210 6.22e-58 182
Msa1401100 AT4G00850.2 51.429 105 46 2 7 106 6 110 1.95e-26 102
Msa1401100 AT4G00850.1 66.667 72 22 1 7 76 6 77 2.92e-26 101
Msa1401100 AT1G01160.1 63.889 72 25 1 2 72 3 74 7.34e-23 92.0
Msa1401100 AT1G01160.2 43.396 106 25 2 2 72 3 108 4.95e-16 74.7
Msa1401100 AT5G28640.2 44.186 172 66 5 63 228 1 148 1.16e-11 61.2
Msa1401100 AT5G28640.3 44.186 172 66 5 63 228 1 148 1.16e-11 61.2

Find 95 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAATTAAGGTAAACTATT+TGG 0.143303 tig0022499:-82040 Msa1401100:three_prime_UTR
GTTTATCTTGTGCTTAATTA+AGG 0.191732 tig0022499:-82052 Msa1401100:three_prime_UTR
TTAGCTGTTCAGATCGAATT+TGG 0.252519 tig0022499:-82220 Msa1401100:three_prime_UTR
GGGTTTGCACAGAAGTCTTA+TGG 0.269289 tig0022499:-82366 Msa1401100:CDS
CTTGGAGGCGGTGGAACTTC+AGG 0.325122 tig0022499:-82500 Msa1401100:CDS
AAGCTAAAATGCATCTGAAT+TGG 0.337794 tig0022499:+82178 None:intergenic
AGAGAGAGAGCATTGGGGTT+GGG 0.342124 tig0022499:+85290 None:intergenic
ACCTGCACCTGTGTTTCCTT+TGG 0.359038 tig0022499:-85137 Msa1401100:five_prime_UTR
TCAACATTGGGTTCTGGTGG+TGG 0.361229 tig0022499:-82419 Msa1401100:CDS
TGATCAGTGGTGACGTTGTT+TGG 0.375156 tig0022499:+84927 None:intergenic
GATTGTAGAGAGTCAGAATT+CGG 0.378404 tig0022499:-83345 Msa1401100:CDS
TTCTGGTGGTGGAGTGATGC+AGG 0.385903 tig0022499:-82663 Msa1401100:CDS
TCATGGGCTGCATCTGCATC+AGG 0.386454 tig0022499:+84964 None:intergenic
GAGAGAGAGCATTGGGGTTG+GGG 0.390988 tig0022499:+85291 None:intergenic
GCTGTTGCTGCGCGAATAGA+AGG 0.398335 tig0022499:+82558 None:intergenic
ATGGTGTAGCAGTACCCTTC+TGG 0.398459 tig0022499:-82680 Msa1401100:intron
TACCTCAAATCTTCTGATGA+TGG 0.413883 tig0022499:-82257 Msa1401100:CDS
TGGATAGTAGGCTGCCATCA+TGG 0.422967 tig0022499:+84947 None:intergenic
CAACATTGGGTTCTGGTGGT+GGG 0.423263 tig0022499:-82418 Msa1401100:CDS
GATGCAGGGTCAGCAGCAGC+AGG 0.427997 tig0022499:-82648 Msa1401100:CDS
GGTGACGTTGTTTGGATAGT+AGG 0.433249 tig0022499:+84935 None:intergenic
AGTGGCAAGCAACAAGAGAT+TGG 0.434502 tig0022499:-82338 Msa1401100:CDS
GGTTTGCACAGAAGTCTTAT+GGG 0.434506 tig0022499:-82365 Msa1401100:CDS
CACCACTGATCACATTCAAC+AGG 0.436615 tig0022499:-84916 Msa1401100:intron
TTGCAAGAAGAGAACTCAAA+AGG 0.437518 tig0022499:-85195 Msa1401100:five_prime_UTR
CATCAGAAGATTTGAGGTAA+AGG 0.438000 tig0022499:+82261 None:intergenic
GGTCGAGGCGGTGGTGGTGA+AGG 0.439472 tig0022499:-82293 Msa1401100:CDS
TCCCATCATCAGAAGATTTG+AGG 0.441249 tig0022499:+82255 None:intergenic
CCGGCCATGGTTGGTGGTTG+AGG 0.446740 tig0022499:+82801 None:intergenic
TAGCAGTACCCTTCTGGTGG+TGG 0.451363 tig0022499:-82674 Msa1401100:CDS
GGATAGTAGGCTGCCATCAT+GGG 0.463215 tig0022499:+84948 None:intergenic
AGTAGCTCAACATTGGGTTC+TGG 0.464044 tig0022499:-82425 Msa1401100:CDS
TCCCACGTTCATATTGCTAC+AGG 0.468413 tig0022499:+82454 None:intergenic
AATTAGTTATTTAGTTAGCT+AGG 0.469547 tig0022499:-82147 Msa1401100:three_prime_UTR
ACCACCAACCATGGCCGGTC+AGG 0.472281 tig0022499:-82796 Msa1401100:intron
GAGAGAGAGAGCATTGGGGT+TGG 0.473989 tig0022499:+85289 None:intergenic
GACACAACAACAACAGCTAA+TGG 0.478063 tig0022499:-82594 Msa1401100:CDS
TCTAGATCAATTAGCTAGCT+AGG 0.481431 tig0022499:-82113 Msa1401100:three_prime_UTR
GCACTTCAACAGCATCAACT+TGG 0.489725 tig0022499:-82518 Msa1401100:CDS
TTCATCCGCGGTGGAGGAGA+GGG 0.494151 tig0022499:-82386 Msa1401100:CDS
TGCTGTTGAAGTGCTGAGTA+AGG 0.496707 tig0022499:+82527 None:intergenic
ACCTCAAATCTTCTGATGAT+GGG 0.498318 tig0022499:-82256 Msa1401100:CDS
CCTGACTTCATCCGCGGTGG+AGG 0.498540 tig0022499:-82392 Msa1401100:CDS
TCCAAAGGAAACACAGGTGC+AGG 0.499472 tig0022499:+85136 None:intergenic
TCGGATCAAGGTCGAGGCGG+TGG 0.500503 tig0022499:-82302 Msa1401100:CDS
ATTGTAGAGAGTCAGAATTC+GGG 0.501834 tig0022499:-83344 Msa1401100:CDS
TGTTTCTACCTGACCGGCCA+TGG 0.501985 tig0022499:+82788 None:intergenic
ACCTGACCGGCCATGGTTGG+TGG 0.503390 tig0022499:+82795 None:intergenic
GTGGCAAGCAACAAGAGATT+GGG 0.506357 tig0022499:-82337 Msa1401100:CDS
GAGTGAGAGACAAGAAAGAA+AGG 0.506535 tig0022499:-85314 Msa1401100:five_prime_UTR
CAGCATCAACTTGGAGGCGG+TGG 0.509178 tig0022499:-82509 Msa1401100:CDS
AGAAGTCTTATGGGAGGTAG+TGG 0.518354 tig0022499:-82356 Msa1401100:CDS
GATCAAGGTCGAGGCGGTGG+TGG 0.520726 tig0022499:-82299 Msa1401100:CDS
GATTGGGATGAGTTCTTCAT+CGG 0.525078 tig0022499:-82321 Msa1401100:CDS
TGCAAGAAGAGAACTCAAAA+GGG 0.527986 tig0022499:-85194 Msa1401100:five_prime_UTR
AAGTGCAGAGGAAGATCCAA+AGG 0.532693 tig0022499:+85121 None:intergenic
AGCTCAACATTGGGTTCTGG+TGG 0.534520 tig0022499:-82422 Msa1401100:CDS
ATGAGTTCTTCATCGGATCA+AGG 0.547272 tig0022499:-82314 Msa1401100:CDS
CTTCATCCGCGGTGGAGGAG+AGG 0.548468 tig0022499:-82387 Msa1401100:CDS
AGGAGGTAGTAGCTCAACAT+TGG 0.549524 tig0022499:-82432 Msa1401100:CDS
CGAGGCGGTGGTGGTGAAGG+CGG 0.550384 tig0022499:-82290 Msa1401100:CDS
GGAGGTAGTAGCTCAACATT+GGG 0.550428 tig0022499:-82431 Msa1401100:CDS
AGCAATATGAACGTGGGAGG+AGG 0.551007 tig0022499:-82449 Msa1401100:CDS
CCTCAACCACCAACCATGGC+CGG 0.554155 tig0022499:-82801 Msa1401100:CDS
CTTCAACAGCATCAACTTGG+AGG 0.554957 tig0022499:-82515 Msa1401100:CDS
TCTACCTGACCGGCCATGGT+TGG 0.555887 tig0022499:+82792 None:intergenic
TGCGTGTGTTTCTACCTGAC+CGG 0.576751 tig0022499:+82782 None:intergenic
TGCATCACTCCACCACCAGA+AGG 0.580900 tig0022499:+82665 None:intergenic
TCTGGTGGTGGAGTGATGCA+GGG 0.593405 tig0022499:-82662 Msa1401100:CDS
TCTTCATCGGATCAAGGTCG+AGG 0.593840 tig0022499:-82308 Msa1401100:CDS
GAGAATCAGCTATTGCAGCT+AGG 0.595794 tig0022499:+82826 None:intergenic
GGAAGATCCAAAGGAAACAC+AGG 0.600293 tig0022499:+85130 None:intergenic
TTTCCTGACTTCATCCGCGG+TGG 0.603311 tig0022499:-82395 Msa1401100:CDS
AACATATCAGGAATCAAGCA+AGG 0.610316 tig0022499:-82869 Msa1401100:intron
ACAAAGTTGAAGAAGTGCAG+AGG 0.613672 tig0022499:+85109 None:intergenic
GGGTTTCCTGACTTCATCCG+CGG 0.614235 tig0022499:-82398 Msa1401100:CDS
GCCTGTAGCAATATGAACGT+GGG 0.617388 tig0022499:-82455 Msa1401100:CDS
GGGAGGAGCACACTACATGC+AGG 0.618698 tig0022499:-82627 Msa1401100:CDS
TCAACCTCAACCACCAACCA+TGG 0.620168 tig0022499:-82805 Msa1401100:CDS
ATGCAGGGTCAGCAGCAGCA+GGG 0.624777 tig0022499:-82647 Msa1401100:CDS
AGAGAGAGATATATAGCTAG+TGG 0.626882 tig0022499:-85015 Msa1401100:five_prime_UTR
CTGTTGCTGCGCGAATAGAA+GGG 0.627676 tig0022499:+82559 None:intergenic
CAACAGCATCAACTTGGAGG+CGG 0.642913 tig0022499:-82512 Msa1401100:CDS
GATGCAGATGCAGCCCATGA+TGG 0.647712 tig0022499:-84961 Msa1401100:CDS
GCATCACTCCACCACCAGAA+GGG 0.647825 tig0022499:+82666 None:intergenic
GTGTAGCAGTACCCTTCTGG+TGG 0.652208 tig0022499:-82677 Msa1401100:intron
AATTAAGCACAAGATAAACA+AGG 0.654592 tig0022499:+82056 None:intergenic
CAGGGTCAGCAGCAGCAGGG+AGG 0.660642 tig0022499:-82644 Msa1401100:CDS
AGCCTGTAGCAATATGAACG+TGG 0.662936 tig0022499:-82456 Msa1401100:CDS
TGTAGCAATATGAACGTGGG+AGG 0.675964 tig0022499:-82452 Msa1401100:CDS
TTGCACAGAAGTCTTATGGG+AGG 0.683269 tig0022499:-82362 Msa1401100:CDS
TTGCTGCGCGAATAGAAGGG+AGG 0.689392 tig0022499:+82562 None:intergenic
TCATCGGATCAAGGTCGAGG+CGG 0.718041 tig0022499:-82305 Msa1401100:CDS
AACCTGTTGAATGTGATCAG+TGG 0.722866 tig0022499:+84914 None:intergenic
GCAAACCCTCTCCTCCACCG+CGG 0.734679 tig0022499:+82381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAAAAATAATATAACGA+TGG + tig0022499:82270-82289 None:intergenic 10.0%
!! TAAAAAATAATTTCTTCATT+AGG - tig0022499:83295-83314 Msa1401100:intron 10.0%
!!! ATGAATCTAATTTTATTTAA+CGG - tig0022499:84250-84269 Msa1401100:intron 10.0%
!! ATTGAATTCAATTGATATAT+AGG + tig0022499:84047-84066 None:intergenic 15.0%
!!! TTCATTAATTTTATGATATG+AGG - tig0022499:84077-84096 Msa1401100:intron 15.0%
!!! TTTTCTATCTTTTCAATATT+GGG + tig0022499:84103-84122 None:intergenic 15.0%
!!! TTTTTCTATCTTTTCAATAT+TGG + tig0022499:84104-84123 None:intergenic 15.0%
!! AATATTGAAAAGATAGAAAA+AGG - tig0022499:84103-84122 Msa1401100:intron 15.0%
!!! ACATTGTGATATAATTTTTT+TGG - tig0022499:84564-84583 Msa1401100:intron 15.0%
!!! TCATAAAATCTTACCATTTT+AGG + tig0022499:83229-83248 None:intergenic 20.0%
!! TCATCATTTGTAATTAGAAA+TGG - tig0022499:83544-83563 Msa1401100:intron 20.0%
!!! ATCTAATTTTATTTAACGGT+TGG - tig0022499:84254-84273 Msa1401100:intron 20.0%
!!! TCTAATTTTATTTAACGGTT+GGG - tig0022499:84255-84274 Msa1401100:intron 20.0%
!! GTATATATGTGTTTATGTAT+CGG + tig0022499:84334-84353 None:intergenic 20.0%
!!! GTTTAATCATTGATTATTGA+TGG - tig0022499:84625-84644 Msa1401100:intron 20.0%
!! AATTAGTTATTTAGTTAGCT+AGG - tig0022499:85177-85196 Msa1401100:five_prime_UTR 20.0%
!! CTTAATTAAGGTAAACTATT+TGG - tig0022499:85284-85303 Msa1401100:five_prime_UTR 20.0%
! TATTACACATCACTTGTATA+TGG - tig0022499:82550-82569 Msa1401100:CDS 25.0%
!!! ACCATTTCATGACTATTTTT+GGG + tig0022499:83474-83493 None:intergenic 25.0%
!! AACCATTTCATGACTATTTT+TGG + tig0022499:83475-83494 None:intergenic 25.0%
! TTGCATAAAATAAGAGAAGA+GGG + tig0022499:83643-83662 None:intergenic 25.0%
! AAATATGCAAACGAATTAAC+CGG + tig0022499:83874-83893 None:intergenic 25.0%
! GGTTGTGTAAAATAAGTAAA+AGG + tig0022499:83925-83944 None:intergenic 25.0%
!! AATTATGCTTGTATTCCTTT+TGG - tig0022499:84184-84203 Msa1401100:intron 25.0%
!! ATTATGCTTGTATTCCTTTT+GGG - tig0022499:84185-84204 Msa1401100:intron 25.0%
! ATGAAGTTTCAGTTCTATAT+GGG + tig0022499:84298-84317 None:intergenic 25.0%
! TATGAAGTTTCAGTTCTATA+TGG + tig0022499:84299-84318 None:intergenic 25.0%
! GAACTGAAACTTCATATATA+AGG - tig0022499:84303-84322 Msa1401100:intron 25.0%
! AATTAAGCACAAGATAAACA+AGG + tig0022499:85271-85290 None:intergenic 25.0%
! GTTTATCTTGTGCTTAATTA+AGG - tig0022499:85272-85291 Msa1401100:five_prime_UTR 25.0%
AGAAGCAAGGAAATTAAAGA+AGG + tig0022499:82477-82496 None:intergenic 30.0%
ACATCACTTGTATATGGATA+TGG - tig0022499:82556-82575 Msa1401100:CDS 30.0%
! GAGAATATTTTGCAGACATA+AGG + tig0022499:82900-82919 None:intergenic 30.0%
ACAAAAATGAATCCAACCAA+AGG + tig0022499:82986-83005 None:intergenic 30.0%
TATACATTCTTATCTCAGGT+AGG + tig0022499:83040-83059 None:intergenic 30.0%
GTCATATACATTCTTATCTC+AGG + tig0022499:83044-83063 None:intergenic 30.0%
TAAGATATGTGAACATAGAG+AGG + tig0022499:83072-83091 None:intergenic 30.0%
TCCCAAAAATAGTCATGAAA+TGG - tig0022499:83470-83489 Msa1401100:intron 30.0%
TAATTAGAAATGGTTGCCTT+GGG - tig0022499:83554-83573 Msa1401100:intron 30.0%
CTAACGTCTTATATCCTAAA+TGG - tig0022499:83580-83599 Msa1401100:intron 30.0%
AAAGTAAGCAAACTCCATTT+AGG + tig0022499:83597-83616 None:intergenic 30.0%
! ACTTTGAAAGTAAGCAAACT+AGG - tig0022499:83612-83631 Msa1401100:intron 30.0%
CTTGCATAAAATAAGAGAAG+AGG + tig0022499:83644-83663 None:intergenic 30.0%
GGATGTTTGAAAGAGAAAAT+TGG + tig0022499:83689-83708 None:intergenic 30.0%
!!! CACATGTTTTAACACAACAA+CGG + tig0022499:83715-83734 None:intergenic 30.0%
TTTCAACACTCACTCTAATT+GGG + tig0022499:83795-83814 None:intergenic 30.0%
! TTTTCAACACTCACTCTAAT+TGG + tig0022499:83796-83815 None:intergenic 30.0%
! TATTTTACACAACCAGTACT+TGG - tig0022499:83931-83950 Msa1401100:intron 30.0%
GAATTAGACTACTACTTACT+CGG + tig0022499:84023-84042 None:intergenic 30.0%
AGATAGAAAAAGGTAAGAGA+TGG - tig0022499:84113-84132 Msa1401100:intron 30.0%
!!! TTGTATTCCTTTTGGGATAA+AGG - tig0022499:84192-84211 Msa1401100:intron 30.0%
TAAAAGACATCAACGTGTTA+GGG + tig0022499:84231-84250 None:intergenic 30.0%
ATAAAAGACATCAACGTGTT+AGG + tig0022499:84232-84251 None:intergenic 30.0%
CATACATGTGATAACATATC+AGG - tig0022499:84443-84462 Msa1401100:intron 30.0%
AAGCTAAAATGCATCTGAAT+TGG + tig0022499:85149-85168 None:intergenic 30.0%
TTGCAAGAAGAGAACTCAAA+AGG - tig0022499:82129-82148 Msa1401100:three_prime_UTR 35.0%
TGCAAGAAGAGAACTCAAAA+GGG - tig0022499:82130-82149 Msa1401100:three_prime_UTR 35.0%
AGAGAGAGATATATAGCTAG+TGG - tig0022499:82309-82328 Msa1401100:CDS 35.0%
!! AACAGAACAGCTTTGTTTGT+GGG - tig0022499:82702-82721 Msa1401100:intron 35.0%
TAAAGTGAAGAGAGTAAAGC+AGG + tig0022499:82805-82824 None:intergenic 35.0%
GACTGAGATAAAGACAAAGA+AGG + tig0022499:83121-83140 None:intergenic 35.0%
!!! GTCACTCACTTTTTTTTGCT+TGG - tig0022499:83138-83157 Msa1401100:intron 35.0%
GTAATTAGAAATGGTTGCCT+TGG - tig0022499:83553-83572 Msa1401100:intron 35.0%
! TATAAGACGTTAGTTTCCCA+AGG + tig0022499:83573-83592 None:intergenic 35.0%
!! GTTTTAACACAACAACGGAA+TGG + tig0022499:83710-83729 None:intergenic 35.0%
GTGTTAAAACATGTGTGTCT+CGG - tig0022499:83720-83739 Msa1401100:intron 35.0%
AGTGGAAACCATTTAGAGAA+TGG - tig0022499:83749-83768 Msa1401100:intron 35.0%
AAACCATTTAGAGAATGGTG+AGG - tig0022499:83754-83773 Msa1401100:intron 35.0%
TAACCTCACCATTCTCTAAA+TGG + tig0022499:83760-83779 None:intergenic 35.0%
GATTGTAGAGAGTCAGAATT+CGG - tig0022499:83979-83998 Msa1401100:intron 35.0%
ATTGTAGAGAGTCAGAATTC+GGG - tig0022499:83980-83999 Msa1401100:intron 35.0%
TTCACATCCTTTATCCCAAA+AGG + tig0022499:84202-84221 None:intergenic 35.0%
GAAACTTCATATATAAGGCG+TGG - tig0022499:84308-84327 Msa1401100:intron 35.0%
AACATATCAGGAATCAAGCA+AGG - tig0022499:84455-84474 Msa1401100:intron 35.0%
! CATCAGAAGATTTGAGGTAA+AGG + tig0022499:85066-85085 None:intergenic 35.0%
TACCTCAAATCTTCTGATGA+TGG - tig0022499:85067-85086 Msa1401100:five_prime_UTR 35.0%
ACCTCAAATCTTCTGATGAT+GGG - tig0022499:85068-85087 Msa1401100:five_prime_UTR 35.0%
TTAGCTGTTCAGATCGAATT+TGG - tig0022499:85104-85123 Msa1401100:five_prime_UTR 35.0%
TCTAGATCAATTAGCTAGCT+AGG - tig0022499:85211-85230 Msa1401100:five_prime_UTR 35.0%
GAGTGAGAGACAAGAAAGAA+AGG - tig0022499:82010-82029 Msa1401100:three_prime_UTR 40.0%
! ACAAAGTTGAAGAAGTGCAG+AGG + tig0022499:82218-82237 None:intergenic 40.0%
AACCTGTTGAATGTGATCAG+TGG + tig0022499:82413-82432 None:intergenic 40.0%
GATATATGCAGACAGAAGCA+AGG + tig0022499:82490-82509 None:intergenic 40.0%
TTACACACAACACAACTTGC+TGG - tig0022499:82635-82654 Msa1401100:CDS 40.0%
TTCTACTGCTCAGCACAAAA+GGG + tig0022499:82684-82703 None:intergenic 40.0%
!! GAACAGAACAGCTTTGTTTG+TGG - tig0022499:82701-82720 Msa1401100:intron 40.0%
TATACCCTCACTTGTTTGCT+AGG - tig0022499:82842-82861 Msa1401100:CDS 40.0%
TCTACCTAGCAAACAAGTGA+GGG + tig0022499:82849-82868 None:intergenic 40.0%
ATCTACCTAGCAAACAAGTG+AGG + tig0022499:82850-82869 None:intergenic 40.0%
ATGCTCTAGCTAATCCTTAG+TGG - tig0022499:82868-82887 Msa1401100:intron 40.0%
AAATGAATCCAACCAAAGGC+AGG + tig0022499:82982-83001 None:intergenic 40.0%
GCTTACCAAGTCTCTTAAGT+GGG + tig0022499:83205-83224 None:intergenic 40.0%
TGCTTACCAAGTCTCTTAAG+TGG + tig0022499:83206-83225 None:intergenic 40.0%
! GACTTGGTAAGCACCTAAAA+TGG - tig0022499:83213-83232 Msa1401100:intron 40.0%
CTCTCATTGTGTCCCAAAAT+AGG - tig0022499:83435-83454 Msa1401100:intron 40.0%
TCATTGTGTCCCAAAATAGG+AGG - tig0022499:83438-83457 Msa1401100:intron 40.0%
CATTGTGTCCCAAAATAGGA+GGG - tig0022499:83439-83458 Msa1401100:intron 40.0%
AAACGAATTAACCGGTGGAA+GGG + tig0022499:83866-83885 None:intergenic 40.0%
TATGCAAACGAATTAACCGG+TGG + tig0022499:83871-83890 None:intergenic 40.0%
ACTTGTTCTCATCCAAGTAC+TGG + tig0022499:83946-83965 None:intergenic 40.0%
ACACGTTGACAAGTCGATAT+TGG + tig0022499:84387-84406 None:intergenic 40.0%
TGTCAACGTGTCTGCTTATT+AGG - tig0022499:84396-84415 Msa1401100:intron 40.0%
GACACAACAACAACAGCTAA+TGG - tig0022499:84730-84749 Msa1401100:intron 40.0%
! GGTTTGCACAGAAGTCTTAT+GGG - tig0022499:84959-84978 Msa1401100:CDS 40.0%
GATTGGGATGAGTTCTTCAT+CGG - tig0022499:85003-85022 Msa1401100:five_prime_UTR 40.0%
ATGAGTTCTTCATCGGATCA+AGG - tig0022499:85010-85029 Msa1401100:five_prime_UTR 40.0%
TCCCATCATCAGAAGATTTG+AGG + tig0022499:85072-85091 None:intergenic 40.0%
!! AGAGAGAGAGAGAGAGCATT+GGG + tig0022499:82043-82062 None:intergenic 45.0%
GGAAGATCCAAAGGAAACAC+AGG + tig0022499:82197-82216 None:intergenic 45.0%
AAGTGCAGAGGAAGATCCAA+AGG + tig0022499:82206-82225 None:intergenic 45.0%
GGTGACGTTGTTTGGATAGT+AGG + tig0022499:82392-82411 None:intergenic 45.0%
! TGATCAGTGGTGACGTTGTT+TGG + tig0022499:82400-82419 None:intergenic 45.0%
CACCACTGATCACATTCAAC+AGG - tig0022499:82408-82427 Msa1401100:CDS 45.0%
GTTCTACTGCTCAGCACAAA+AGG + tig0022499:82685-82704 None:intergenic 45.0%
AGAGAGTAAAGCAGGTAGGT+AGG + tig0022499:82797-82816 None:intergenic 45.0%
GTGAAGAGAGTAAAGCAGGT+AGG + tig0022499:82801-82820 None:intergenic 45.0%
TGCTTCCCACTTAAGAGACT+TGG - tig0022499:83197-83216 Msa1401100:intron 45.0%
! ATGCTTATAGAGTACCCCGA+CGG - tig0022499:83319-83338 Msa1401100:intron 45.0%
! ATCTTGTTAACGAGTGCCGT+CGG + tig0022499:83338-83357 None:intergenic 45.0%
TGTCCCAAAATAGGAGGGAA+AGG - tig0022499:83444-83463 Msa1401100:intron 45.0%
! CAGCCTTTCCCTCCTATTTT+GGG + tig0022499:83450-83469 None:intergenic 45.0%
! ACAGCCTTTCCCTCCTATTT+TGG + tig0022499:83451-83470 None:intergenic 45.0%
TTTGTCACTATCCCTTCCAC+CGG - tig0022499:83852-83871 Msa1401100:intron 45.0%
CAAACGAATTAACCGGTGGA+AGG + tig0022499:83867-83886 None:intergenic 45.0%
TACGTAGCAGCATCAGAAGA+TGG + tig0022499:84150-84169 None:intergenic 45.0%
GAGAATCAGCTATTGCAGCT+AGG + tig0022499:84501-84520 None:intergenic 45.0%
!! TGCTGTTGAAGTGCTGAGTA+AGG + tig0022499:84800-84819 None:intergenic 45.0%
GCACTTCAACAGCATCAACT+TGG - tig0022499:84806-84825 Msa1401100:intron 45.0%
CTTCAACAGCATCAACTTGG+AGG - tig0022499:84809-84828 Msa1401100:intron 45.0%
AGCCTGTAGCAATATGAACG+TGG - tig0022499:84868-84887 Msa1401100:intron 45.0%
GCCTGTAGCAATATGAACGT+GGG - tig0022499:84869-84888 Msa1401100:intron 45.0%
TCCCACGTTCATATTGCTAC+AGG + tig0022499:84873-84892 None:intergenic 45.0%
TGTAGCAATATGAACGTGGG+AGG - tig0022499:84872-84891 Msa1401100:intron 45.0%
AGGAGGTAGTAGCTCAACAT+TGG - tig0022499:84892-84911 Msa1401100:intron 45.0%
GGAGGTAGTAGCTCAACATT+GGG - tig0022499:84893-84912 Msa1401100:intron 45.0%
AGTAGCTCAACATTGGGTTC+TGG - tig0022499:84899-84918 Msa1401100:intron 45.0%
GGGTTTGCACAGAAGTCTTA+TGG - tig0022499:84958-84977 Msa1401100:CDS 45.0%
! TTGCACAGAAGTCTTATGGG+AGG - tig0022499:84962-84981 Msa1401100:CDS 45.0%
! AGAAGTCTTATGGGAGGTAG+TGG - tig0022499:84968-84987 Msa1401100:CDS 45.0%
AGTGGCAAGCAACAAGAGAT+TGG - tig0022499:84986-85005 Msa1401100:exon 45.0%
GTGGCAAGCAACAAGAGATT+GGG - tig0022499:84987-85006 Msa1401100:exon 45.0%
!! AAATAAAATAAAATAAATGA+AGG + tig0022499:82517-82536 None:intergenic 5.0%
!! AATATAATAGTAATAAATAA+AGG + tig0022499:82769-82788 None:intergenic 5.0%
!! AGAGAGAGAGCATTGGGGTT+GGG + tig0022499:82037-82056 None:intergenic 50.0%
!! GAGAGAGAGAGAGAGCATTG+GGG + tig0022499:82042-82061 None:intergenic 50.0%
! GAGAGAGAGAGAGAGAGCAT+TGG + tig0022499:82044-82063 None:intergenic 50.0%
ACCTGCACCTGTGTTTCCTT+TGG - tig0022499:82187-82206 Msa1401100:three_prime_UTR 50.0%
TCCAAAGGAAACACAGGTGC+AGG + tig0022499:82191-82210 None:intergenic 50.0%
GGATAGTAGGCTGCCATCAT+GGG + tig0022499:82379-82398 None:intergenic 50.0%
TGGATAGTAGGCTGCCATCA+TGG + tig0022499:82380-82399 None:intergenic 50.0%
TGGCATCTTTCTGTGCTTCC+TGG - tig0022499:82655-82674 Msa1401100:CDS 50.0%
GGCATCTTTCTGTGCTTCCT+GGG - tig0022499:82656-82675 Msa1401100:CDS 50.0%
GCATCTTTCTGTGCTTCCTG+GGG - tig0022499:82657-82676 Msa1401100:CDS 50.0%
CATCTTTCTGTGCTTCCTGG+GGG - tig0022499:82658-82677 Msa1401100:CDS 50.0%
ACATAAGGCAGCAGCCACTA+AGG + tig0022499:82885-82904 None:intergenic 50.0%
GGGTGGGTGAGTTATAGAGT+GGG + tig0022499:82952-82971 None:intergenic 50.0%
TGGGTGGGTGAGTTATAGAG+TGG + tig0022499:82953-82972 None:intergenic 50.0%
AATCCAACCAAAGGCAGGCA+GGG + tig0022499:82977-82996 None:intergenic 50.0%
TTATCTCAGGTAGGAGCAGC+AGG + tig0022499:83031-83050 None:intergenic 50.0%
! TCTTGTTAACGAGTGCCGTC+GGG + tig0022499:83337-83356 None:intergenic 50.0%
TGTGTGTCTCGGCTCTTAAG+TGG - tig0022499:83731-83750 Msa1401100:intron 50.0%
! GGTTGGGTTGCTGAAGTGAA+GGG - tig0022499:84271-84290 Msa1401100:intron 50.0%
TCAACCTCAACCACCAACCA+TGG - tig0022499:84519-84538 Msa1401100:intron 50.0%
! TGCGTGTGTTTCTACCTGAC+CGG + tig0022499:84545-84564 None:intergenic 50.0%
ATGGTGTAGCAGTACCCTTC+TGG - tig0022499:84644-84663 Msa1401100:intron 50.0%
CTGTTGCTGCGCGAATAGAA+GGG + tig0022499:84768-84787 None:intergenic 50.0%
CAACAGCATCAACTTGGAGG+CGG - tig0022499:84812-84831 Msa1401100:intron 50.0%
AGCAATATGAACGTGGGAGG+AGG - tig0022499:84875-84894 Msa1401100:intron 50.0%
! AGCTCAACATTGGGTTCTGG+TGG - tig0022499:84902-84921 Msa1401100:intron 50.0%
! TCAACATTGGGTTCTGGTGG+TGG - tig0022499:84905-84924 Msa1401100:intron 50.0%
! CAACATTGGGTTCTGGTGGT+GGG - tig0022499:84906-84925 Msa1401100:intron 50.0%
TCTTCATCGGATCAAGGTCG+AGG - tig0022499:85016-85035 Msa1401100:five_prime_UTR 50.0%
!! GAGAGAGAGCATTGGGGTTG+GGG + tig0022499:82036-82055 None:intergenic 55.0%
!! GAGAGAGAGAGCATTGGGGT+TGG + tig0022499:82038-82057 None:intergenic 55.0%
! TCATGGGCTGCATCTGCATC+AGG + tig0022499:82363-82382 None:intergenic 55.0%
GATGCAGATGCAGCCCATGA+TGG - tig0022499:82363-82382 Msa1401100:CDS 55.0%
TAGAGTGGGCACAGTGCAAC+TGG + tig0022499:82938-82957 None:intergenic 55.0%
AACCAAAGGCAGGCAGGGAT+GGG + tig0022499:82972-82991 None:intergenic 55.0%
GAATCCAACCAAAGGCAGGC+AGG + tig0022499:82978-82997 None:intergenic 55.0%
! CTTGTTAACGAGTGCCGTCG+GGG + tig0022499:83336-83355 None:intergenic 55.0%
! CGGTTGGGTTGCTGAAGTGA+AGG - tig0022499:84270-84289 Msa1401100:intron 55.0%
! TGTTTCTACCTGACCGGCCA+TGG + tig0022499:84539-84558 None:intergenic 55.0%
GTGTAGCAGTACCCTTCTGG+TGG - tig0022499:84647-84666 Msa1401100:intron 55.0%
TAGCAGTACCCTTCTGGTGG+TGG - tig0022499:84650-84669 Msa1401100:intron 55.0%
GCATCACTCCACCACCAGAA+GGG + tig0022499:84661-84680 None:intergenic 55.0%
TGCATCACTCCACCACCAGA+AGG + tig0022499:84662-84681 None:intergenic 55.0%
!! TTCTGGTGGTGGAGTGATGC+AGG - tig0022499:84661-84680 Msa1401100:intron 55.0%
!! TCTGGTGGTGGAGTGATGCA+GGG - tig0022499:84662-84681 Msa1401100:intron 55.0%
TTGCTGCGCGAATAGAAGGG+AGG + tig0022499:84765-84784 None:intergenic 55.0%
GCTGTTGCTGCGCGAATAGA+AGG + tig0022499:84769-84788 None:intergenic 55.0%
GGGTTTCCTGACTTCATCCG+CGG - tig0022499:84926-84945 Msa1401100:CDS 55.0%
TTTCCTGACTTCATCCGCGG+TGG - tig0022499:84929-84948 Msa1401100:CDS 55.0%
TCATCGGATCAAGGTCGAGG+CGG - tig0022499:85019-85038 Msa1401100:five_prime_UTR 55.0%
AAAGGCAGGCAGGGATGGGT+GGG + tig0022499:82968-82987 None:intergenic 60.0%
CAACCAAAGGCAGGCAGGGA+TGG + tig0022499:82973-82992 None:intergenic 60.0%
CATCCCTGCCTGCCTTTGGT+TGG - tig0022499:82971-82990 Msa1401100:intron 60.0%
CCTCAACCACCAACCATGGC+CGG - tig0022499:84523-84542 Msa1401100:intron 60.0%
TCTACCTGACCGGCCATGGT+TGG + tig0022499:84535-84554 None:intergenic 60.0%
ATGCAGGGTCAGCAGCAGCA+GGG - tig0022499:84677-84696 Msa1401100:intron 60.0%
! GGGAGGAGCACACTACATGC+AGG - tig0022499:84697-84716 Msa1401100:intron 60.0%
CAGCATCAACTTGGAGGCGG+TGG - tig0022499:84815-84834 Msa1401100:intron 60.0%
CTTGGAGGCGGTGGAACTTC+AGG - tig0022499:84824-84843 Msa1401100:intron 60.0%
TTCATCCGCGGTGGAGGAGA+GGG - tig0022499:84938-84957 Msa1401100:CDS 60.0%
CTCAGCACAAAAGGGCCCCC+AGG + tig0022499:82676-82695 None:intergenic 65.0%
CAAAGGCAGGCAGGGATGGG+TGG + tig0022499:82969-82988 None:intergenic 65.0%
CACCCATCCCTGCCTGCCTT+TGG - tig0022499:82967-82986 Msa1401100:intron 65.0%
!! CCGGCCATGGTTGGTGGTTG+AGG + tig0022499:84526-84545 None:intergenic 65.0%
ACCACCAACCATGGCCGGTC+AGG - tig0022499:84528-84547 Msa1401100:intron 65.0%
ACCTGACCGGCCATGGTTGG+TGG + tig0022499:84532-84551 None:intergenic 65.0%
GATGCAGGGTCAGCAGCAGC+AGG - tig0022499:84676-84695 Msa1401100:intron 65.0%
CCTCCACCGCGGATGAAGTC+AGG + tig0022499:84935-84954 None:intergenic 65.0%
CCTGACTTCATCCGCGGTGG+AGG - tig0022499:84932-84951 Msa1401100:CDS 65.0%
CTTCATCCGCGGTGGAGGAG+AGG - tig0022499:84937-84956 Msa1401100:CDS 65.0%
GCAAACCCTCTCCTCCACCG+CGG + tig0022499:84946-84965 None:intergenic 65.0%
TCGGATCAAGGTCGAGGCGG+TGG - tig0022499:85022-85041 Msa1401100:five_prime_UTR 65.0%
GATCAAGGTCGAGGCGGTGG+TGG - tig0022499:85025-85044 Msa1401100:five_prime_UTR 65.0%
CAGGGTCAGCAGCAGCAGGG+AGG - tig0022499:84680-84699 Msa1401100:intron 70.0%
! GGTCGAGGCGGTGGTGGTGA+AGG - tig0022499:85031-85050 Msa1401100:five_prime_UTR 70.0%
! CGAGGCGGTGGTGGTGAAGG+CGG - tig0022499:85034-85053 Msa1401100:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
tig0022499 gene 81988 85358 81988 ID=Msa1401100;Name=Msa1401100
tig0022499 mRNA 81988 85358 81988 ID=Msa1401100-mRNA-1;Parent=Msa1401100;Name=Msa1401100-mRNA-1;_AED=0.04;_eAED=0.04;_QI=361|0.66|0.75|1|1|1|4|262|228
tig0022499 exon 81988 82693 81988 ID=Msa1401100-mRNA-1:exon:18100;Parent=Msa1401100-mRNA-1
tig0022499 exon 82797 82881 82797 ID=Msa1401100-mRNA-1:exon:18099;Parent=Msa1401100-mRNA-1
tig0022499 exon 83323 83399 83323 ID=Msa1401100-mRNA-1:exon:18098;Parent=Msa1401100-mRNA-1
tig0022499 exon 84917 85358 84917 ID=Msa1401100-mRNA-1:exon:18097;Parent=Msa1401100-mRNA-1
tig0022499 five_prime_UTR 84998 85358 84998 ID=Msa1401100-mRNA-1:five_prime_utr;Parent=Msa1401100-mRNA-1
tig0022499 CDS 84917 84997 84917 ID=Msa1401100-mRNA-1:cds;Parent=Msa1401100-mRNA-1
tig0022499 CDS 83323 83399 83323 ID=Msa1401100-mRNA-1:cds;Parent=Msa1401100-mRNA-1
tig0022499 CDS 82797 82881 82797 ID=Msa1401100-mRNA-1:cds;Parent=Msa1401100-mRNA-1
tig0022499 CDS 82250 82693 82250 ID=Msa1401100-mRNA-1:cds;Parent=Msa1401100-mRNA-1
tig0022499 three_prime_UTR 81988 82249 81988 ID=Msa1401100-mRNA-1:three_prime_utr;Parent=Msa1401100-mRNA-1
Gene Sequence

>Msa1401100

ATGCAGCAGCACCTGATGCAGATGCAGCCCATGATGGCAGCCTACTATCCAAACAACGTCACCACTGATCACATTCAACAGTACTTGGATGAGAACAAGTCTTTGATTCTGAAGATTGTAGAGAGTCAGAATTCGGGCAAGCTTAACGAGTGTGCCGAGAATCAAGCAAGGCTACAGAGAAATCTCATGTACCTAGCTGCAATAGCTGATTCTCAACCTCAACCACCAACCATGGCCGGTCAGCAGTACCCTTCTGGTGGTGGAGTGATGCAGGGTCAGCAGCAGCAGGGAGGAGCACACTACATGCAGGCTCAGCAGATGACACAACAACAACAGCTAATGGCTGCACGCTCCTCCCTTCTATTCGCGCAGCAACAGCAGCAGCAACCTTACTCAGCACTTCAACAGCATCAACTTGGAGGCGGTGGAACTTCAGGACTACACATGATGCAAAGCGAAGCCTGTAGCAATATGAACGTGGGAGGAGGTAGTAGCTCAACATTGGGTTCTGGTGGTGGGTTTCCTGACTTCATCCGCGGTGGAGGAGAGGGTTTGCACAGAAGTCTTATGGGAGGTAGTGGCAAGCAACAAGAGATTGGGATGAGTTCTTCATCGGATCAAGGTCGAGGCGGTGGTGGTGAAGGCGGCGAAAACCTTTACCTCAAATCTTCTGATGATGGGAACTAG
Protein sequence

>Msa1401100

MQQHLMQMQPMMAAYYPNNVTTDHIQQYLDENKSLILKIVESQNSGKLNECAENQARLQRNLMYLAAIADSQPQPPTMAGQQYPSGGGVMQGQQQQGGAHYMQAQQMTQQQQLMAARSSLLFAQQQQQQPYSALQQHQLGGGGTSGLHMMQSEACSNMNVGGGSSSTLGSGGGFPDFIRGGGEGLHRSLMGGSGKQQEIGMSSSSDQGRGGGGEGGENLYLKSSDDGN*