AlfalfaGEDB | Msa1442690

M. sativa cultivar zhongmu-4 / Msa1442690

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1442690	sp\|Q9SZL8\|FRS5_ARATH	41.250	640	367	8	41	674	71	707	1.14e-165	498

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1442690	A0A396JW00	96.741	675	21	1	4	677	12	686	0.0	1372

TFs/TRs:

Gene ID	Type	Classification
Msa1442690	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0083460	Msa1442690	0.918836	9.519930e-87	-8.615850e-47
Msa1441630	Msa1442690	0.962237	1.223061e-120	-8.615850e-47
Msa0610670	Msa1442690	0.814711	1.340203e-51	-8.615850e-47
Msa0648880	Msa1442690	0.800645	1.307640e-48	-8.615850e-47
Msa0657970	Msa1442690	0.803331	3.669127e-49	-8.615850e-47
Msa0685750	Msa1442690	0.812897	3.363455e-51	-8.615850e-47
Msa0711790	Msa1442690	0.822252	2.616663e-53	-8.615850e-47
Msa0830530	Msa1442690	0.814805	1.278046e-51	-8.615850e-47
Msa0132760	Msa1442690	0.914956	1.041804e-84	-8.615850e-47
Msa0237310	Msa1442690	0.812410	4.298697e-51	-8.615850e-47
Msa0439970	Msa1442690	0.811870	5.640483e-51	-8.615850e-47
Msa0274730	Msa1442690	0.814294	1.657808e-51	-8.615850e-47
Msa0315530	Msa1442690	0.812899	3.360684e-51	-8.615850e-47
Msa0338110	Msa1442690	0.802876	4.555811e-49	-8.615850e-47
Msa0579200	Msa1442690	0.832507	9.088072e-56	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1442690	MtrunA17_Chr1g0202341	96.741	675	21	1	4	677	12	686	0.0	1372
Msa1442690	MtrunA17_Chr5g0448761	41.601	637	355	7	36	660	53	684	1.27e-170	507
Msa1442690	MtrunA17_Chr5g0440451	34.627	644	380	9	42	664	42	665	4.72e-122	385
Msa1442690	MtrunA17_Chr1g0207551	34.335	632	401	6	41	669	32	652	5.54e-122	382
Msa1442690	MtrunA17_Chr5g0448511	38.612	461	282	1	204	664	1	460	1.69e-117	362
Msa1442690	MtrunA17_Chr2g0324011	33.943	601	373	10	37	625	4	592	1.76e-116	361
Msa1442690	MtrunA17_Chr2g0296781	37.478	571	328	13	47	602	1	557	6.41e-116	364
Msa1442690	MtrunA17_Chr5g0441581	34.380	605	367	12	9	603	1	585	1.11e-115	362
Msa1442690	MtrunA17_Chr2g0329031	33.601	622	389	9	42	659	13	614	2.38e-115	363
Msa1442690	MtrunA17_Chr3g0080351	33.182	660	393	11	16	656	43	673	3.84e-114	362
Msa1442690	MtrunA17_Chr2g0287061	35.247	627	349	13	41	660	181	757	6.98e-114	360
Msa1442690	MtrunA17_Chr8g0363841	33.604	616	372	13	27	626	5	599	1.27e-112	353
Msa1442690	MtrunA17_Chr3g0118611	38.627	466	278	3	203	664	101	562	1.17e-110	351
Msa1442690	MtrunA17_Chr2g0291581	31.204	673	427	11	5	664	34	683	8.51e-109	344
Msa1442690	MtrunA17_Chr1g0203721	31.062	631	424	6	46	668	66	693	1.24e-102	333
Msa1442690	MtrunA17_Chr6g0458391	32.149	563	356	6	43	601	225	765	2.23e-101	327
Msa1442690	MtrunA17_Chr6g0458391	31.288	163	96	5	23	177	76	230	9.33e-13	71.6
Msa1442690	MtrunA17_Chr2g0287651	31.690	568	375	7	42	603	74	634	6.28e-101	328
Msa1442690	MtrunA17_Chr6g0477261	38.182	440	261	6	172	602	9	446	1.20e-100	322
Msa1442690	MtrunA17_Chr3g0082121	32.154	622	401	7	26	642	26	631	1.70e-97	315
Msa1442690	MtrunA17_Chr1g0196541	33.451	568	355	9	47	602	1	557	2.95e-97	315
Msa1442690	MtrunA17_Chr1g0203051	29.196	572	387	8	24	589	63	622	6.80e-91	296
Msa1442690	MtrunA17_Chr2g0291591	33.268	511	323	8	42	545	63	562	7.07e-91	293
Msa1442690	MtrunA17_Chr1g0198761	30.096	628	409	13	45	654	49	664	3.02e-84	281
Msa1442690	MtrunA17_Chr2g0276841	28.571	630	420	13	43	660	70	681	1.09e-74	254
Msa1442690	MtrunA17_Chr2g0290391	30.382	576	369	11	41	598	49	610	1.77e-70	242
Msa1442690	MtrunA17_Chr2g0294841	26.822	645	435	13	19	638	14	646	1.80e-68	238
Msa1442690	MtrunA17_Chr1g0164591	37.171	304	180	3	134	435	14	308	3.99e-63	213
Msa1442690	MtrunA17_Chr5g0431001	30.044	456	296	10	19	455	33	484	1.33e-57	203
Msa1442690	MtrunA17_Chr7g0258861	37.063	286	173	5	42	322	9	292	3.87e-56	193
Msa1442690	MtrunA17_Chr4g0052331	55.789	95	42	0	1	95	1	95	8.16e-28	107
Msa1442690	MtrunA17_Chr3g0077241	60.870	92	32	2	6	96	1	89	1.15e-27	108
Msa1442690	MtrunA17_Chr2g0329361	44.954	109	59	1	41	148	59	167	2.37e-24	102
Msa1442690	MtrunA17_Chr2g0308841	27.232	224	156	3	381	600	6	226	6.63e-24	102
Msa1442690	MtrunA17_Chr2g0329421	38.596	114	58	3	42	150	26	132	5.68e-19	86.3
Msa1442690	MtrunA17_Chr4g0033611	33.582	134	77	3	17	145	50	176	6.61e-18	84.0
Msa1442690	MtrunA17_Chr1g0149131	32.727	110	74	0	321	430	1	110	2.38e-16	75.9
Msa1442690	MtrunA17_Chr3g0078041	32.738	168	107	5	7	170	12	177	8.87e-16	77.4
Msa1442690	MtrunA17_Chr7g0233911	32.812	128	82	3	42	168	33	157	5.06e-13	68.9
Msa1442690	MtrunA17_Chr6g0451441	26.794	209	139	6	3	208	50	247	7.13e-11	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1442690	AT4G38180.1	41.250	640	367	8	41	674	71	707	1.16e-166	498
Msa1442690	AT4G15090.1	35.275	618	385	8	41	653	49	656	2.01e-127	397
Msa1442690	AT4G15090.5	35.275	618	385	8	41	653	49	656	2.01e-127	397
Msa1442690	AT4G15090.3	35.275	618	385	8	41	653	49	656	2.01e-127	397
Msa1442690	AT4G15090.2	35.275	618	385	8	41	653	49	656	2.01e-127	397
Msa1442690	AT4G15090.4	35.275	618	385	8	41	653	49	656	2.01e-127	397
Msa1442690	AT5G18960.2	38.078	562	303	9	42	601	210	728	6.72e-125	389
Msa1442690	AT5G18960.2	32.075	106	56	2	41	145	41	131	2.79e-12	70.5
Msa1442690	AT5G18960.1	38.078	562	303	9	42	601	210	728	6.72e-125	389
Msa1442690	AT5G18960.1	32.075	106	56	2	41	145	41	131	2.79e-12	70.5
Msa1442690	AT3G06250.2	36.649	573	314	8	32	601	178	704	7.33e-124	386
Msa1442690	AT3G06250.2	32.456	114	61	2	33	145	18	116	3.78e-13	73.6
Msa1442690	AT3G06250.1	36.649	573	314	8	32	601	178	704	7.33e-124	386
Msa1442690	AT3G06250.1	32.456	114	61	2	33	145	18	116	3.78e-13	73.6
Msa1442690	AT3G06250.3	36.649	573	314	8	32	601	178	704	7.33e-124	386
Msa1442690	AT3G06250.3	32.456	114	61	2	33	145	18	116	3.78e-13	73.6
Msa1442690	AT2G27110.2	34.350	623	376	8	42	663	48	638	6.77e-123	386
Msa1442690	AT2G27110.1	34.350	623	376	8	42	663	48	638	6.77e-123	386
Msa1442690	AT3G22170.2	31.379	631	391	9	41	658	68	669	5.93e-113	359
Msa1442690	AT3G22170.1	31.379	631	391	9	41	658	68	669	5.93e-113	359
Msa1442690	AT2G27110.3	38.667	450	269	3	215	663	50	493	4.76e-111	351
Msa1442690	AT1G76320.3	31.942	623	388	9	47	659	1	597	6.32e-103	328
Msa1442690	AT1G76320.1	31.942	623	388	9	47	659	1	597	2.31e-102	329
Msa1442690	AT1G76320.4	31.942	623	388	9	47	659	1	597	2.31e-102	329
Msa1442690	AT1G76320.2	32.149	619	392	10	47	659	1	597	2.83e-102	328
Msa1442690	AT1G52520.1	30.236	635	410	11	43	664	85	699	1.16e-100	323
Msa1442690	AT4G38170.1	35.973	442	276	3	220	660	21	456	1.05e-97	311
Msa1442690	AT4G19990.3	30.769	637	381	9	41	659	19	613	4.97e-94	307
Msa1442690	AT4G19990.4	30.769	637	381	9	41	659	19	613	4.97e-94	307
Msa1442690	AT4G19990.5	30.722	651	386	10	41	668	19	627	6.12e-94	307
Msa1442690	AT2G32250.5	31.495	562	338	6	46	604	41	558	6.87e-94	307
Msa1442690	AT4G19990.7	30.769	637	381	9	41	659	33	627	6.96e-94	307
Msa1442690	AT2G32250.2	31.673	562	337	6	46	604	41	558	1.38e-93	307
Msa1442690	AT2G32250.4	31.673	562	337	6	46	604	41	558	1.38e-93	307
Msa1442690	AT2G32250.6	31.673	562	337	6	46	604	41	558	1.55e-93	307
Msa1442690	AT2G32250.1	31.673	562	337	6	46	604	41	558	1.55e-93	307
Msa1442690	AT2G32250.3	31.673	562	337	6	46	604	41	558	1.55e-93	307
Msa1442690	AT4G19990.6	30.722	651	386	10	41	668	19	627	1.61e-93	306
Msa1442690	AT4G19990.2	30.722	651	386	10	41	668	19	627	1.61e-93	306
Msa1442690	AT2G32250.8	31.373	561	338	6	47	604	1	517	7.34e-93	305
Msa1442690	AT2G32250.7	31.373	561	338	6	47	604	1	517	7.74e-93	305
Msa1442690	AT1G80010.1	29.787	658	399	16	39	663	93	720	9.25e-93	303
Msa1442690	AT4G19990.1	30.822	584	353	9	94	668	29	570	2.53e-85	283
Msa1442690	AT5G28530.1	27.646	633	426	12	43	655	55	675	5.38e-74	252
Msa1442690	AT5G28530.3	27.330	633	424	12	43	655	55	671	8.35e-73	249
Msa1442690	AT5G28530.2	27.330	633	424	12	43	655	55	671	8.35e-73	249
Msa1442690	AT1G10240.2	27.832	618	407	14	6	598	1	604	9.36e-72	246
Msa1442690	AT1G10240.1	27.832	618	407	14	6	598	1	604	9.36e-72	246
Msa1442690	AT5G28530.4	27.520	367	260	3	289	655	252	612	2.93e-45	171
Msa1442690	AT5G28530.5	26.975	367	258	3	289	655	252	608	3.33e-44	168
Msa1442690	AT3G59470.3	38.053	113	58	3	42	149	69	174	2.61e-18	85.5
Msa1442690	AT3G59470.1	38.053	113	58	3	42	149	69	174	2.61e-18	85.5
Msa1442690	AT3G59470.2	38.053	113	58	3	42	149	81	186	3.40e-18	85.5
Msa1442690	AT3G07500.1	32.298	161	83	4	37	173	27	185	1.09e-14	74.3
Msa1442690	AT4G12850.4	34.286	105	63	2	42	145	10	109	1.97e-13	68.6
Msa1442690	AT2G43280.1	34.959	123	77	3	32	152	11	132	2.23e-13	70.1
Msa1442690	AT4G12850.2	34.286	105	63	2	42	145	10	109	1.10e-12	66.2
Msa1442690	AT4G12850.1	34.286	105	63	2	42	145	10	109	1.61e-12	67.0

Find 94 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTGGCGGTATTGATAATTT+TGG	0.195979	tig0030153:+6815	Msa1442690:CDS
GAAAATTAACTAATTGCTTT+TGG	0.203998	tig0030153:+6987	Msa1442690:CDS
CATTCCTTCAATGGGCTCTA+AGG	0.236305	tig0030153:-6375	None:intergenic
TGATGATTAACTCCAGTAAT+TGG	0.238736	tig0030153:-7106	None:intergenic
TGAAAAGTCACATGATATTT+AGG	0.266210	tig0030153:-7889	None:intergenic
AGAGTTGCTTACAAATATTT+TGG	0.269630	tig0030153:+7025	Msa1442690:CDS
AATTGTCAGTTGAAGAATAC+TGG	0.271394	tig0030153:+6932	Msa1442690:CDS
CGAAGAGACTGTGCACTTCT+AGG	0.276999	tig0030153:-6698	None:intergenic
AACTGTGTTCCTAAAGAAAA+AGG	0.312218	tig0030153:+7984	Msa1442690:CDS
AATCGTTTGGTATTCTTTGT+AGG	0.315386	tig0030153:+7953	Msa1442690:CDS
TATACTCAATTCGATCAAAA+TGG	0.317025	tig0030153:+7497	Msa1442690:CDS
CAATATGCCACATGCAATAA+TGG	0.325491	tig0030153:-7306	None:intergenic
TTCATTGTTTAATAGTGTTA+TGG	0.327096	tig0030153:+8122	Msa1442690:CDS
ATTCTTCAATTGTGATAGAT+TGG	0.351090	tig0030153:-7408	None:intergenic
ACTGATGTGCTTGATGAATC+TGG	0.354525	tig0030153:+6758	Msa1442690:CDS
GAGAAAACAGCTGGTGATTA+TGG	0.357013	tig0030153:-6676	None:intergenic
TGTCAAAAGTTTGAATCGTT+TGG	0.358658	tig0030153:+7940	Msa1442690:CDS
TTTAAACTCCATTCCTTCAA+TGG	0.364249	tig0030153:-6384	None:intergenic
AGAACAAACGGTATGATAAT+TGG	0.370543	tig0030153:+6491	Msa1442690:CDS
TAGTGTTATGGTTCGTTCAC+TGG	0.382958	tig0030153:+8134	Msa1442690:CDS
ATTCCTTCAATGGGCTCTAA+GGG	0.384475	tig0030153:-6374	None:intergenic
CAATAATGGTGATTAACCTT+TGG	0.392421	tig0030153:-7292	None:intergenic
TTGAAGAATTTGAGTCTGAA+TGG	0.400537	tig0030153:+7422	Msa1442690:CDS
AGTAATTGATAACATCTTGT+TGG	0.416145	tig0030153:-6844	None:intergenic
TTAAACTCCATTCCTTCAAT+GGG	0.435121	tig0030153:-6383	None:intergenic
GTGCTTGATGAATCTGGTTT+AGG	0.447135	tig0030153:+6764	Msa1442690:CDS
TTGACAATTGAAGAATTTGA+AGG	0.456352	tig0030153:+8078	Msa1442690:CDS
CTTGGTTAGAAGCAATGAAT+GGG	0.462263	tig0030153:+7203	Msa1442690:CDS
TTGTTTGGTTGCTAAGTACT+TGG	0.462493	tig0030153:+7185	Msa1442690:CDS
TGAAGAATTTGAGTCTGAAT+GGG	0.463681	tig0030153:+7423	Msa1442690:CDS
TCCTTCAATGGGCTCTAAGG+GGG	0.466966	tig0030153:-6372	None:intergenic
TAGATCTGATTCATGTGCTC+TGG	0.470489	tig0030153:-7340	None:intergenic
CTGGAATGTCTACCACTCAT+AGG	0.472850	tig0030153:+7551	Msa1442690:CDS
TTTGGCTCGAAACCCCTCCT+TGG	0.475441	tig0030153:-6531	None:intergenic
ACTCCAGTAATTGGAACAAA+AGG	0.475504	tig0030153:-7097	None:intergenic
TTTGAAGCTTCTTCTTCCAT+GGG	0.477189	tig0030153:-7736	None:intergenic
ACTGTTTGCGTTATTATGGC+AGG	0.480651	tig0030153:+6242	None:intergenic
TTATTGCATGTGGCATATTG+AGG	0.485948	tig0030153:+7309	Msa1442690:CDS
ACTTGGTTAGAAGCAATGAA+TGG	0.491300	tig0030153:+7202	Msa1442690:CDS
CACATGAATCAGATCTATCA+TGG	0.493856	tig0030153:+7346	Msa1442690:CDS
GAAGCAATGATTGATAAATA+CGG	0.502447	tig0030153:+7445	Msa1442690:CDS
AAATTATGATGTACATATGC+AGG	0.504291	tig0030153:-7523	None:intergenic
GGGTATAAAGTTCGCAAAAG+TGG	0.509142	tig0030153:-7715	None:intergenic
TCAAGAAATTCTTTGTTCCA+AGG	0.511520	tig0030153:+6514	Msa1442690:CDS
CCTTCAATGGGCTCTAAGGG+GGG	0.511902	tig0030153:-6371	None:intergenic
AAGCAATGATTGATAAATAC+GGG	0.513517	tig0030153:+7446	Msa1442690:CDS
CACAGTATGTTCTACAACGA+TGG	0.518628	tig0030153:+8022	Msa1442690:CDS
TGTGTCAGAAACTTGCTCAA+TGG	0.523310	tig0030153:-7622	None:intergenic
CTTAGAGCCCATTGAAGGAA+TGG	0.526994	tig0030153:+6376	Msa1442690:CDS
ACAATTGAAGAATTTGAAGG+AGG	0.530417	tig0030153:+8081	Msa1442690:CDS
CACTTCTAGGAGAAAACAGC+TGG	0.530595	tig0030153:-6685	None:intergenic
TGAGATATTTCAAGATGAGT+TGG	0.531340	tig0030153:+7780	Msa1442690:CDS
GTTACTAAGTCAAGAACAAA+CGG	0.531428	tig0030153:+6479	Msa1442690:CDS
AAATTCTTTGTTCCAAGGAG+GGG	0.533751	tig0030153:+6519	Msa1442690:CDS
AGGTCATGAGATGACAGATA+TGG	0.541561	tig0030153:+6262	Msa1442690:CDS
GATGGTGGTTCACTATTGAG+AGG	0.542446	tig0030153:-6344	None:intergenic
ATATTTCAAGATGAGTTGGT+TGG	0.542621	tig0030153:+7784	Msa1442690:CDS
ATTGTCAGTTGAAGAATACT+GGG	0.543680	tig0030153:+6933	Msa1442690:CDS
CTATCACCAATGCAGTTGCA+AGG	0.546795	tig0030153:+7263	Msa1442690:CDS
GAAATTCTTTGTTCCAAGGA+GGG	0.547884	tig0030153:+6518	Msa1442690:CDS
TGCACGTTACAACGAGGAAA+GGG	0.548977	tig0030153:+7663	Msa1442690:CDS
TGAAACCAGGGTGGGATGCA+AGG	0.555494	tig0030153:+6595	Msa1442690:CDS
AAACACCCTTGCAACTGCAT+TGG	0.561204	tig0030153:-7269	None:intergenic
TCTGTCTTATGCACTGAAAG+TGG	0.562246	tig0030153:+6797	Msa1442690:CDS
TTCCTTCAATGGGCTCTAAG+GGG	0.577393	tig0030153:-6373	None:intergenic
TTAATCACCATTATTGCATG+TGG	0.578974	tig0030153:+7299	Msa1442690:CDS
CCGTGCCTTGCATCCCACCC+TGG	0.580963	tig0030153:-6600	None:intergenic
TTTGCGAACTTTATACCCCA+TGG	0.583317	tig0030153:+7720	Msa1442690:CDS
AGTTGCAAGGGTGTTTCCAA+AGG	0.585018	tig0030153:+7276	Msa1442690:CDS
ATGCACGTTACAACGAGGAA+AGG	0.585794	tig0030153:+7662	Msa1442690:CDS
CGTTGTAGAACATACTGTGA+AGG	0.588365	tig0030153:-8018	None:intergenic
CATGAGATGACAGATATGGA+TGG	0.601625	tig0030153:+6266	Msa1442690:CDS
AGAAATTCTTTGTTCCAAGG+AGG	0.606491	tig0030153:+6517	Msa1442690:CDS
TTGAAGCTTCTTCTTCCATG+GGG	0.606500	tig0030153:-7735	None:intergenic
CATTGGTGATAGCAGTGTGT+TGG	0.606939	tig0030153:-7252	None:intergenic
GTTGTAGAACATACTGTGAA+GGG	0.621697	tig0030153:-8017	None:intergenic
GCGTTTCAAATGGATGCCGA+AGG	0.622605	tig0030153:+6965	Msa1442690:CDS
AGAGCTTGAAGAATCAAATG+AGG	0.626048	tig0030153:+8245	Msa1442690:CDS
TATCACCAATGCAGTTGCAA+GGG	0.633978	tig0030153:+7264	Msa1442690:CDS
TTATTACACATGATGAAACC+AGG	0.637175	tig0030153:+6582	Msa1442690:CDS
CATACTTTCACTCCTATGAG+TGG	0.639566	tig0030153:-7563	None:intergenic
ACAAGGTACGCTCAGAACAA+AGG	0.639870	tig0030153:+6434	Msa1442690:CDS
CCAGGGTGGGATGCAAGGCA+CGG	0.641442	tig0030153:+6600	Msa1442690:CDS
ACGGGTTGCAAGATAATCAG+TGG	0.648204	tig0030153:+7464	Msa1442690:CDS
TTGGTTAGAAGCAATGAATG+GGG	0.648562	tig0030153:+7204	Msa1442690:CDS
AAGCAAAGAGTTACTACACA+AGG	0.651539	tig0030153:+6417	Msa1442690:CDS
ACACATGATGAAACCAGGGT+GGG	0.653979	tig0030153:+6587	Msa1442690:CDS
ACTTAGTAACACGGCCCATG+CGG	0.656322	tig0030153:-6466	None:intergenic
GTCTTATGCACTGAAAGTGG+CGG	0.656902	tig0030153:+6800	Msa1442690:CDS
TTGTTCTTGACTTAGTAACA+CGG	0.665948	tig0030153:-6475	None:intergenic
TACACATGATGAAACCAGGG+TGG	0.688016	tig0030153:+6586	Msa1442690:CDS
TATTACACATGATGAAACCA+GGG	0.694710	tig0030153:+6583	Msa1442690:CDS
TGTATCATTTGAGACAACCA+TGG	0.701765	tig0030153:-6297	None:intergenic
TCTTGATGCACGTTACAACG+AGG	0.755842	tig0030153:+7657	Msa1442690:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAGAAAAATAATGATATA+TGG	+	tig0030153:6630-6649	Msa1442690:CDS	10.0%
!!	TAAAGAAAAATAATGATATA+TGG	+	tig0030153:6630-6649	Msa1442690:CDS	10.0%
!!!	AAAATTAACTAATTGCTTTT+GGG	+	tig0030153:6988-7007	Msa1442690:CDS	15.0%
!!!	TATTTATTTTTCAAGTATGT+TGG	-	tig0030153:7070-7089	None:intergenic	15.0%
!!!	AAGTATGAATAAGTTTTTTA+AGG	+	tig0030153:7579-7598	Msa1442690:CDS	15.0%
!!!	AAAATTAACTAATTGCTTTT+GGG	+	tig0030153:6988-7007	Msa1442690:CDS	15.0%
!!!	TATTTATTTTTCAAGTATGT+TGG	-	tig0030153:7070-7089	None:intergenic	15.0%
!!!	AAGTATGAATAAGTTTTTTA+AGG	+	tig0030153:7579-7598	Msa1442690:CDS	15.0%
!!!	GAAAATTAACTAATTGCTTT+TGG	+	tig0030153:6987-7006	Msa1442690:CDS	20.0%
!!!	ATTTTTCTTTCTTAGCATTT+CGG	-	tig0030153:8050-8069	None:intergenic	20.0%
!!	TTCATTGTTTAATAGTGTTA+TGG	+	tig0030153:8122-8141	Msa1442690:CDS	20.0%
!!!	GAAAATTAACTAATTGCTTT+TGG	+	tig0030153:6987-7006	Msa1442690:CDS	20.0%
!!!	ATTTTTCTTTCTTAGCATTT+CGG	-	tig0030153:8050-8069	None:intergenic	20.0%
!!	TTCATTGTTTAATAGTGTTA+TGG	+	tig0030153:8122-8141	Msa1442690:CDS	20.0%
!!	AGTAATTGATAACATCTTGT+TGG	-	tig0030153:6847-6866	None:intergenic	25.0%
!!	GCAATTAGTTAATTTTCCTT+CGG	-	tig0030153:6984-7003	None:intergenic	25.0%
!!	AGAGTTGCTTACAAATATTT+TGG	+	tig0030153:7025-7044	Msa1442690:CDS	25.0%
!!!	TTACAAATATTTTGGTGATG+TGG	+	tig0030153:7033-7052	Msa1442690:CDS	25.0%
!!	CATCAACAATCAATTCTTTT+TGG	+	tig0030153:7127-7146	Msa1442690:CDS	25.0%
!	ATTCTTCAATTGTGATAGAT+TGG	-	tig0030153:7411-7430	None:intergenic	25.0%
!	GAAGCAATGATTGATAAATA+CGG	+	tig0030153:7445-7464	Msa1442690:CDS	25.0%
!	AAGCAATGATTGATAAATAC+GGG	+	tig0030153:7446-7465	Msa1442690:CDS	25.0%
!	TATACTCAATTCGATCAAAA+TGG	+	tig0030153:7497-7516	Msa1442690:CDS	25.0%
!	AAATTATGATGTACATATGC+AGG	-	tig0030153:7526-7545	None:intergenic	25.0%
!	GAAGCTTCAAAAATTTATAC+AGG	+	tig0030153:7748-7767	Msa1442690:CDS	25.0%
!!!	AGAAAATTCAACTTTTTCTG+TGG	-	tig0030153:7824-7843	None:intergenic	25.0%
!	TGAAAAGTCACATGATATTT+AGG	-	tig0030153:7892-7911	None:intergenic	25.0%
!!	TTTTTCTTTCTTAGCATTTC+GGG	-	tig0030153:8049-8068	None:intergenic	25.0%
!	GCTAAGAAAGAAAAATTTGA+AGG	+	tig0030153:8054-8073	Msa1442690:CDS	25.0%
!	TTGACAATTGAAGAATTTGA+AGG	+	tig0030153:8078-8097	Msa1442690:CDS	25.0%
!!	AGTAATTGATAACATCTTGT+TGG	-	tig0030153:6847-6866	None:intergenic	25.0%
!!	GCAATTAGTTAATTTTCCTT+CGG	-	tig0030153:6984-7003	None:intergenic	25.0%
!!	AGAGTTGCTTACAAATATTT+TGG	+	tig0030153:7025-7044	Msa1442690:CDS	25.0%
!!!	TTACAAATATTTTGGTGATG+TGG	+	tig0030153:7033-7052	Msa1442690:CDS	25.0%
!!	CATCAACAATCAATTCTTTT+TGG	+	tig0030153:7127-7146	Msa1442690:CDS	25.0%
!	ATTCTTCAATTGTGATAGAT+TGG	-	tig0030153:7411-7430	None:intergenic	25.0%
!	GAAGCAATGATTGATAAATA+CGG	+	tig0030153:7445-7464	Msa1442690:CDS	25.0%
!	AAGCAATGATTGATAAATAC+GGG	+	tig0030153:7446-7465	Msa1442690:CDS	25.0%
!	TATACTCAATTCGATCAAAA+TGG	+	tig0030153:7497-7516	Msa1442690:CDS	25.0%
!	AAATTATGATGTACATATGC+AGG	-	tig0030153:7526-7545	None:intergenic	25.0%
!	GAAGCTTCAAAAATTTATAC+AGG	+	tig0030153:7748-7767	Msa1442690:CDS	25.0%
!!!	AGAAAATTCAACTTTTTCTG+TGG	-	tig0030153:7824-7843	None:intergenic	25.0%
!	TGAAAAGTCACATGATATTT+AGG	-	tig0030153:7892-7911	None:intergenic	25.0%
!!	TTTTTCTTTCTTAGCATTTC+GGG	-	tig0030153:8049-8068	None:intergenic	25.0%
!	GCTAAGAAAGAAAAATTTGA+AGG	+	tig0030153:8054-8073	Msa1442690:CDS	25.0%
!	TTGACAATTGAAGAATTTGA+AGG	+	tig0030153:8078-8097	Msa1442690:CDS	25.0%
	TTAAACTCCATTCCTTCAAT+GGG	-	tig0030153:6386-6405	None:intergenic	30.0%
	TTTAAACTCCATTCCTTCAA+TGG	-	tig0030153:6387-6406	None:intergenic	30.0%
	TTGTTCTTGACTTAGTAACA+CGG	-	tig0030153:6478-6497	None:intergenic	30.0%
	GTTACTAAGTCAAGAACAAA+CGG	+	tig0030153:6479-6498	Msa1442690:CDS	30.0%
	AGAACAAACGGTATGATAAT+TGG	+	tig0030153:6491-6510	Msa1442690:CDS	30.0%
	TCAAGAAATTCTTTGTTCCA+AGG	+	tig0030153:6514-6533	Msa1442690:CDS	30.0%
!!	TCCTTGTTTCACATACTTTT+TGG	-	tig0030153:6552-6571	None:intergenic	30.0%
	TTATTACACATGATGAAACC+AGG	+	tig0030153:6582-6601	Msa1442690:CDS	30.0%
	TATTACACATGATGAAACCA+GGG	+	tig0030153:6583-6602	Msa1442690:CDS	30.0%
	AATTGTCAGTTGAAGAATAC+TGG	+	tig0030153:6932-6951	Msa1442690:CDS	30.0%
	ATTGTCAGTTGAAGAATACT+GGG	+	tig0030153:6933-6952	Msa1442690:CDS	30.0%
!	GTTTTTCTATGCGTTTCAAA+TGG	+	tig0030153:6955-6974	Msa1442690:CDS	30.0%
	TGATGATTAACTCCAGTAAT+TGG	-	tig0030153:7109-7128	None:intergenic	30.0%
!!!	AAACTGTAGAGAGTTTTGTT+TGG	+	tig0030153:7170-7189	Msa1442690:CDS	30.0%
!!	GGTCTGTGATAATAGTTTTA+GGG	-	tig0030153:7234-7253	None:intergenic	30.0%
!	TGGTCTGTGATAATAGTTTT+AGG	-	tig0030153:7235-7254	None:intergenic	30.0%
	CAATAATGGTGATTAACCTT+TGG	-	tig0030153:7295-7314	None:intergenic	30.0%
	TTAATCACCATTATTGCATG+TGG	+	tig0030153:7299-7318	Msa1442690:CDS	30.0%
	TTGAAGAATTTGAGTCTGAA+TGG	+	tig0030153:7422-7441	Msa1442690:CDS	30.0%
	TGAAGAATTTGAGTCTGAAT+GGG	+	tig0030153:7423-7442	Msa1442690:CDS	30.0%
!	GTACATCATAATTTTTGTGC+TGG	+	tig0030153:7532-7551	Msa1442690:CDS	30.0%
	TGAGATATTTCAAGATGAGT+TGG	+	tig0030153:7780-7799	Msa1442690:CDS	30.0%
!!	ATATTTCAAGATGAGTTGGT+TGG	+	tig0030153:7784-7803	Msa1442690:CDS	30.0%
!	AGTTGAATTTTCTGATGAAG+TGG	+	tig0030153:7831-7850	Msa1442690:CDS	30.0%
	TGTCAAAAGTTTGAATCGTT+TGG	+	tig0030153:7940-7959	Msa1442690:CDS	30.0%
	AATCGTTTGGTATTCTTTGT+AGG	+	tig0030153:7953-7972	Msa1442690:CDS	30.0%
	AACTGTGTTCCTAAAGAAAA+AGG	+	tig0030153:7984-8003	Msa1442690:CDS	30.0%
!	GATAACGTACCTTTTTCTTT+AGG	-	tig0030153:7996-8015	None:intergenic	30.0%
	ACAATTGAAGAATTTGAAGG+AGG	+	tig0030153:8081-8100	Msa1442690:CDS	30.0%
!!	AATTTTCTGAAAGAGCTTCA+TGG	+	tig0030153:8157-8176	Msa1442690:CDS	30.0%
	TTAAACTCCATTCCTTCAAT+GGG	-	tig0030153:6386-6405	None:intergenic	30.0%
	TTTAAACTCCATTCCTTCAA+TGG	-	tig0030153:6387-6406	None:intergenic	30.0%
	TTGTTCTTGACTTAGTAACA+CGG	-	tig0030153:6478-6497	None:intergenic	30.0%
	GTTACTAAGTCAAGAACAAA+CGG	+	tig0030153:6479-6498	Msa1442690:CDS	30.0%
	AGAACAAACGGTATGATAAT+TGG	+	tig0030153:6491-6510	Msa1442690:CDS	30.0%
	TCAAGAAATTCTTTGTTCCA+AGG	+	tig0030153:6514-6533	Msa1442690:CDS	30.0%
!!	TCCTTGTTTCACATACTTTT+TGG	-	tig0030153:6552-6571	None:intergenic	30.0%
	TTATTACACATGATGAAACC+AGG	+	tig0030153:6582-6601	Msa1442690:CDS	30.0%
	TATTACACATGATGAAACCA+GGG	+	tig0030153:6583-6602	Msa1442690:CDS	30.0%
	AATTGTCAGTTGAAGAATAC+TGG	+	tig0030153:6932-6951	Msa1442690:CDS	30.0%
	ATTGTCAGTTGAAGAATACT+GGG	+	tig0030153:6933-6952	Msa1442690:CDS	30.0%
!	GTTTTTCTATGCGTTTCAAA+TGG	+	tig0030153:6955-6974	Msa1442690:CDS	30.0%
	TGATGATTAACTCCAGTAAT+TGG	-	tig0030153:7109-7128	None:intergenic	30.0%
!!!	AAACTGTAGAGAGTTTTGTT+TGG	+	tig0030153:7170-7189	Msa1442690:CDS	30.0%
!!	GGTCTGTGATAATAGTTTTA+GGG	-	tig0030153:7234-7253	None:intergenic	30.0%
!	TGGTCTGTGATAATAGTTTT+AGG	-	tig0030153:7235-7254	None:intergenic	30.0%
	CAATAATGGTGATTAACCTT+TGG	-	tig0030153:7295-7314	None:intergenic	30.0%
	TTAATCACCATTATTGCATG+TGG	+	tig0030153:7299-7318	Msa1442690:CDS	30.0%
	TTGAAGAATTTGAGTCTGAA+TGG	+	tig0030153:7422-7441	Msa1442690:CDS	30.0%
	TGAAGAATTTGAGTCTGAAT+GGG	+	tig0030153:7423-7442	Msa1442690:CDS	30.0%
!	GTACATCATAATTTTTGTGC+TGG	+	tig0030153:7532-7551	Msa1442690:CDS	30.0%
	TGAGATATTTCAAGATGAGT+TGG	+	tig0030153:7780-7799	Msa1442690:CDS	30.0%
!!	ATATTTCAAGATGAGTTGGT+TGG	+	tig0030153:7784-7803	Msa1442690:CDS	30.0%
!	AGTTGAATTTTCTGATGAAG+TGG	+	tig0030153:7831-7850	Msa1442690:CDS	30.0%
	TGTCAAAAGTTTGAATCGTT+TGG	+	tig0030153:7940-7959	Msa1442690:CDS	30.0%
	AATCGTTTGGTATTCTTTGT+AGG	+	tig0030153:7953-7972	Msa1442690:CDS	30.0%
	AACTGTGTTCCTAAAGAAAA+AGG	+	tig0030153:7984-8003	Msa1442690:CDS	30.0%
!	GATAACGTACCTTTTTCTTT+AGG	-	tig0030153:7996-8015	None:intergenic	30.0%
	ACAATTGAAGAATTTGAAGG+AGG	+	tig0030153:8081-8100	Msa1442690:CDS	30.0%
!!	AATTTTCTGAAAGAGCTTCA+TGG	+	tig0030153:8157-8176	Msa1442690:CDS	30.0%
!	TATGGATGGAGATTTTACCA+TGG	+	tig0030153:6280-6299	Msa1442690:CDS	35.0%
!	TGTATCATTTGAGACAACCA+TGG	-	tig0030153:6300-6319	None:intergenic	35.0%
	AAGCAAAGAGTTACTACACA+AGG	+	tig0030153:6417-6436	Msa1442690:CDS	35.0%
!!!	AAAGGTTTTAGTTTCCGCAT+GGG	+	tig0030153:6452-6471	Msa1442690:CDS	35.0%
	AGAAATTCTTTGTTCCAAGG+AGG	+	tig0030153:6517-6536	Msa1442690:CDS	35.0%
	GAAATTCTTTGTTCCAAGGA+GGG	+	tig0030153:6518-6537	Msa1442690:CDS	35.0%
	AAATTCTTTGTTCCAAGGAG+GGG	+	tig0030153:6519-6538	Msa1442690:CDS	35.0%
!	GCCAAAAAGTATGTGAAACA+AGG	+	tig0030153:6548-6567	Msa1442690:CDS	35.0%
!!!	AGTGGCGGTATTGATAATTT+TGG	+	tig0030153:6815-6834	Msa1442690:CDS	35.0%
!!!	ATTGCTTTTGGGTTGATTCA+AGG	+	tig0030153:6999-7018	Msa1442690:CDS	35.0%
!	ACTCCTTTTGTTCCAATTAC+TGG	+	tig0030153:7094-7113	Msa1442690:CDS	35.0%
	ACTCCAGTAATTGGAACAAA+AGG	-	tig0030153:7100-7119	None:intergenic	35.0%
	TTGTTTGGTTGCTAAGTACT+TGG	+	tig0030153:7185-7204	Msa1442690:CDS	35.0%
	ACTTGGTTAGAAGCAATGAA+TGG	+	tig0030153:7202-7221	Msa1442690:CDS	35.0%
	CTTGGTTAGAAGCAATGAAT+GGG	+	tig0030153:7203-7222	Msa1442690:CDS	35.0%
	TTGGTTAGAAGCAATGAATG+GGG	+	tig0030153:7204-7223	Msa1442690:CDS	35.0%
	CAATATGCCACATGCAATAA+TGG	-	tig0030153:7309-7328	None:intergenic	35.0%
	TTATTGCATGTGGCATATTG+AGG	+	tig0030153:7309-7328	Msa1442690:CDS	35.0%
	CACATGAATCAGATCTATCA+TGG	+	tig0030153:7346-7365	Msa1442690:CDS	35.0%
!	TTTGAAGCTTCTTCTTCCAT+GGG	-	tig0030153:7739-7758	None:intergenic	35.0%
!!!	TTTTGAAGCTTCTTCTTCCA+TGG	-	tig0030153:7740-7759	None:intergenic	35.0%
	GTTGTAGAACATACTGTGAA+GGG	-	tig0030153:8020-8039	None:intergenic	35.0%
	AGAGCTTGAAGAATCAAATG+AGG	+	tig0030153:8245-8264	Msa1442690:CDS	35.0%
!	TATGGATGGAGATTTTACCA+TGG	+	tig0030153:6280-6299	Msa1442690:CDS	35.0%
!	TGTATCATTTGAGACAACCA+TGG	-	tig0030153:6300-6319	None:intergenic	35.0%
	AAGCAAAGAGTTACTACACA+AGG	+	tig0030153:6417-6436	Msa1442690:CDS	35.0%
!!!	AAAGGTTTTAGTTTCCGCAT+GGG	+	tig0030153:6452-6471	Msa1442690:CDS	35.0%
	AGAAATTCTTTGTTCCAAGG+AGG	+	tig0030153:6517-6536	Msa1442690:CDS	35.0%
	GAAATTCTTTGTTCCAAGGA+GGG	+	tig0030153:6518-6537	Msa1442690:CDS	35.0%
	AAATTCTTTGTTCCAAGGAG+GGG	+	tig0030153:6519-6538	Msa1442690:CDS	35.0%
!	GCCAAAAAGTATGTGAAACA+AGG	+	tig0030153:6548-6567	Msa1442690:CDS	35.0%
!!!	AGTGGCGGTATTGATAATTT+TGG	+	tig0030153:6815-6834	Msa1442690:CDS	35.0%
!!!	ATTGCTTTTGGGTTGATTCA+AGG	+	tig0030153:6999-7018	Msa1442690:CDS	35.0%
!	ACTCCTTTTGTTCCAATTAC+TGG	+	tig0030153:7094-7113	Msa1442690:CDS	35.0%
	ACTCCAGTAATTGGAACAAA+AGG	-	tig0030153:7100-7119	None:intergenic	35.0%
	TTGTTTGGTTGCTAAGTACT+TGG	+	tig0030153:7185-7204	Msa1442690:CDS	35.0%
	ACTTGGTTAGAAGCAATGAA+TGG	+	tig0030153:7202-7221	Msa1442690:CDS	35.0%
	CTTGGTTAGAAGCAATGAAT+GGG	+	tig0030153:7203-7222	Msa1442690:CDS	35.0%
	TTGGTTAGAAGCAATGAATG+GGG	+	tig0030153:7204-7223	Msa1442690:CDS	35.0%
	CAATATGCCACATGCAATAA+TGG	-	tig0030153:7309-7328	None:intergenic	35.0%
	TTATTGCATGTGGCATATTG+AGG	+	tig0030153:7309-7328	Msa1442690:CDS	35.0%
	CACATGAATCAGATCTATCA+TGG	+	tig0030153:7346-7365	Msa1442690:CDS	35.0%
!	TTTGAAGCTTCTTCTTCCAT+GGG	-	tig0030153:7739-7758	None:intergenic	35.0%
!!!	TTTTGAAGCTTCTTCTTCCA+TGG	-	tig0030153:7740-7759	None:intergenic	35.0%
	GTTGTAGAACATACTGTGAA+GGG	-	tig0030153:8020-8039	None:intergenic	35.0%
	AGAGCTTGAAGAATCAAATG+AGG	+	tig0030153:8245-8264	Msa1442690:CDS	35.0%
	AGGTCATGAGATGACAGATA+TGG	+	tig0030153:6262-6281	Msa1442690:CDS	40.0%
	CATGAGATGACAGATATGGA+TGG	+	tig0030153:6266-6285	Msa1442690:CDS	40.0%
!	ATTCCTTCAATGGGCTCTAA+GGG	-	tig0030153:6377-6396	None:intergenic	40.0%
!!!	CAAAGGTTTTAGTTTCCGCA+TGG	+	tig0030153:6451-6470	Msa1442690:CDS	40.0%
	GAGAAAACAGCTGGTGATTA+TGG	-	tig0030153:6679-6698	None:intergenic	40.0%
	ACTGATGTGCTTGATGAATC+TGG	+	tig0030153:6758-6777	Msa1442690:CDS	40.0%
!	GTGCTTGATGAATCTGGTTT+AGG	+	tig0030153:6764-6783	Msa1442690:CDS	40.0%
	TCTGTCTTATGCACTGAAAG+TGG	+	tig0030153:6797-6816	Msa1442690:CDS	40.0%
!!!	TTTTTGGTTGTGCTCTCTTG+TGG	+	tig0030153:7143-7162	Msa1442690:CDS	40.0%
!!	TTTTGGTTGTGCTCTCTTGT+GGG	+	tig0030153:7144-7163	Msa1442690:CDS	40.0%
	TATCACCAATGCAGTTGCAA+GGG	+	tig0030153:7264-7283	Msa1442690:CDS	40.0%
	TAGATCTGATTCATGTGCTC+TGG	-	tig0030153:7343-7362	None:intergenic	40.0%
	CATACTTTCACTCCTATGAG+TGG	-	tig0030153:7566-7585	None:intergenic	40.0%
!	TGTGTCAGAAACTTGCTCAA+TGG	-	tig0030153:7625-7644	None:intergenic	40.0%
	GGGTATAAAGTTCGCAAAAG+TGG	-	tig0030153:7718-7737	None:intergenic	40.0%
	TTTGCGAACTTTATACCCCA+TGG	+	tig0030153:7720-7739	Msa1442690:CDS	40.0%
	TTGAAGCTTCTTCTTCCATG+GGG	-	tig0030153:7738-7757	None:intergenic	40.0%
	CGTTGTAGAACATACTGTGA+AGG	-	tig0030153:8021-8040	None:intergenic	40.0%
	CACAGTATGTTCTACAACGA+TGG	+	tig0030153:8022-8041	Msa1442690:CDS	40.0%
	TAGTGTTATGGTTCGTTCAC+TGG	+	tig0030153:8134-8153	Msa1442690:CDS	40.0%
	AGGTCATGAGATGACAGATA+TGG	+	tig0030153:6262-6281	Msa1442690:CDS	40.0%
	CATGAGATGACAGATATGGA+TGG	+	tig0030153:6266-6285	Msa1442690:CDS	40.0%
!	ATTCCTTCAATGGGCTCTAA+GGG	-	tig0030153:6377-6396	None:intergenic	40.0%
!!!	CAAAGGTTTTAGTTTCCGCA+TGG	+	tig0030153:6451-6470	Msa1442690:CDS	40.0%
	GAGAAAACAGCTGGTGATTA+TGG	-	tig0030153:6679-6698	None:intergenic	40.0%
	ACTGATGTGCTTGATGAATC+TGG	+	tig0030153:6758-6777	Msa1442690:CDS	40.0%
!	GTGCTTGATGAATCTGGTTT+AGG	+	tig0030153:6764-6783	Msa1442690:CDS	40.0%
	TCTGTCTTATGCACTGAAAG+TGG	+	tig0030153:6797-6816	Msa1442690:CDS	40.0%
!!!	TTTTTGGTTGTGCTCTCTTG+TGG	+	tig0030153:7143-7162	Msa1442690:CDS	40.0%
!!	TTTTGGTTGTGCTCTCTTGT+GGG	+	tig0030153:7144-7163	Msa1442690:CDS	40.0%
	TATCACCAATGCAGTTGCAA+GGG	+	tig0030153:7264-7283	Msa1442690:CDS	40.0%
	TAGATCTGATTCATGTGCTC+TGG	-	tig0030153:7343-7362	None:intergenic	40.0%
	CATACTTTCACTCCTATGAG+TGG	-	tig0030153:7566-7585	None:intergenic	40.0%
!	TGTGTCAGAAACTTGCTCAA+TGG	-	tig0030153:7625-7644	None:intergenic	40.0%
	GGGTATAAAGTTCGCAAAAG+TGG	-	tig0030153:7718-7737	None:intergenic	40.0%
	TTTGCGAACTTTATACCCCA+TGG	+	tig0030153:7720-7739	Msa1442690:CDS	40.0%
	TTGAAGCTTCTTCTTCCATG+GGG	-	tig0030153:7738-7757	None:intergenic	40.0%
	CGTTGTAGAACATACTGTGA+AGG	-	tig0030153:8021-8040	None:intergenic	40.0%
	CACAGTATGTTCTACAACGA+TGG	+	tig0030153:8022-8041	Msa1442690:CDS	40.0%
	TAGTGTTATGGTTCGTTCAC+TGG	+	tig0030153:8134-8153	Msa1442690:CDS	40.0%
!	GATGGTGGTTCACTATTGAG+AGG	-	tig0030153:6347-6366	None:intergenic	45.0%
!!	TTCCTTCAATGGGCTCTAAG+GGG	-	tig0030153:6376-6395	None:intergenic	45.0%
!	CATTCCTTCAATGGGCTCTA+AGG	-	tig0030153:6378-6397	None:intergenic	45.0%
	CTTAGAGCCCATTGAAGGAA+TGG	+	tig0030153:6376-6395	Msa1442690:CDS	45.0%
	ACAAGGTACGCTCAGAACAA+AGG	+	tig0030153:6434-6453	Msa1442690:CDS	45.0%
	TACACATGATGAAACCAGGG+TGG	+	tig0030153:6586-6605	Msa1442690:CDS	45.0%
	ACACATGATGAAACCAGGGT+GGG	+	tig0030153:6587-6606	Msa1442690:CDS	45.0%
!	CACTTCTAGGAGAAAACAGC+TGG	-	tig0030153:6688-6707	None:intergenic	45.0%
	GTCTTATGCACTGAAAGTGG+CGG	+	tig0030153:6800-6819	Msa1442690:CDS	45.0%
!	CATTGGTGATAGCAGTGTGT+TGG	-	tig0030153:7255-7274	None:intergenic	45.0%
	CTATCACCAATGCAGTTGCA+AGG	+	tig0030153:7263-7282	Msa1442690:CDS	45.0%
	AAACACCCTTGCAACTGCAT+TGG	-	tig0030153:7272-7291	None:intergenic	45.0%
!!	AGTTGCAAGGGTGTTTCCAA+AGG	+	tig0030153:7276-7295	Msa1442690:CDS	45.0%
!	ACGGGTTGCAAGATAATCAG+TGG	+	tig0030153:7464-7483	Msa1442690:CDS	45.0%
	CTGGAATGTCTACCACTCAT+AGG	+	tig0030153:7551-7570	Msa1442690:CDS	45.0%
	TCTTGATGCACGTTACAACG+AGG	+	tig0030153:7657-7676	Msa1442690:CDS	45.0%
	ATGCACGTTACAACGAGGAA+AGG	+	tig0030153:7662-7681	Msa1442690:CDS	45.0%
	TGCACGTTACAACGAGGAAA+GGG	+	tig0030153:7663-7682	Msa1442690:CDS	45.0%
!	GATGGTGGTTCACTATTGAG+AGG	-	tig0030153:6347-6366	None:intergenic	45.0%
!!	TTCCTTCAATGGGCTCTAAG+GGG	-	tig0030153:6376-6395	None:intergenic	45.0%
!	CATTCCTTCAATGGGCTCTA+AGG	-	tig0030153:6378-6397	None:intergenic	45.0%
	CTTAGAGCCCATTGAAGGAA+TGG	+	tig0030153:6376-6395	Msa1442690:CDS	45.0%
	ACAAGGTACGCTCAGAACAA+AGG	+	tig0030153:6434-6453	Msa1442690:CDS	45.0%
	TACACATGATGAAACCAGGG+TGG	+	tig0030153:6586-6605	Msa1442690:CDS	45.0%
	ACACATGATGAAACCAGGGT+GGG	+	tig0030153:6587-6606	Msa1442690:CDS	45.0%
!	CACTTCTAGGAGAAAACAGC+TGG	-	tig0030153:6688-6707	None:intergenic	45.0%
	GTCTTATGCACTGAAAGTGG+CGG	+	tig0030153:6800-6819	Msa1442690:CDS	45.0%
!	CATTGGTGATAGCAGTGTGT+TGG	-	tig0030153:7255-7274	None:intergenic	45.0%
	CTATCACCAATGCAGTTGCA+AGG	+	tig0030153:7263-7282	Msa1442690:CDS	45.0%
	AAACACCCTTGCAACTGCAT+TGG	-	tig0030153:7272-7291	None:intergenic	45.0%
!!	AGTTGCAAGGGTGTTTCCAA+AGG	+	tig0030153:7276-7295	Msa1442690:CDS	45.0%
!	ACGGGTTGCAAGATAATCAG+TGG	+	tig0030153:7464-7483	Msa1442690:CDS	45.0%
	CTGGAATGTCTACCACTCAT+AGG	+	tig0030153:7551-7570	Msa1442690:CDS	45.0%
	TCTTGATGCACGTTACAACG+AGG	+	tig0030153:7657-7676	Msa1442690:CDS	45.0%
	ATGCACGTTACAACGAGGAA+AGG	+	tig0030153:7662-7681	Msa1442690:CDS	45.0%
	TGCACGTTACAACGAGGAAA+GGG	+	tig0030153:7663-7682	Msa1442690:CDS	45.0%
!!	TCCTTCAATGGGCTCTAAGG+GGG	-	tig0030153:6375-6394	None:intergenic	50.0%
	ACTTAGTAACACGGCCCATG+CGG	-	tig0030153:6469-6488	None:intergenic	50.0%
	CGAAGAGACTGTGCACTTCT+AGG	-	tig0030153:6701-6720	None:intergenic	50.0%
	GCGTTTCAAATGGATGCCGA+AGG	+	tig0030153:6965-6984	Msa1442690:CDS	50.0%
!!	TCCTTCAATGGGCTCTAAGG+GGG	-	tig0030153:6375-6394	None:intergenic	50.0%
	ACTTAGTAACACGGCCCATG+CGG	-	tig0030153:6469-6488	None:intergenic	50.0%
	CGAAGAGACTGTGCACTTCT+AGG	-	tig0030153:6701-6720	None:intergenic	50.0%
	GCGTTTCAAATGGATGCCGA+AGG	+	tig0030153:6965-6984	Msa1442690:CDS	50.0%
!!	CCTTCAATGGGCTCTAAGGG+GGG	-	tig0030153:6374-6393	None:intergenic	55.0%
!	TTTGGCTCGAAACCCCTCCT+TGG	-	tig0030153:6534-6553	None:intergenic	55.0%
	TGAAACCAGGGTGGGATGCA+AGG	+	tig0030153:6595-6614	Msa1442690:CDS	55.0%
!!	CCTTCAATGGGCTCTAAGGG+GGG	-	tig0030153:6374-6393	None:intergenic	55.0%
!	TTTGGCTCGAAACCCCTCCT+TGG	-	tig0030153:6534-6553	None:intergenic	55.0%
	TGAAACCAGGGTGGGATGCA+AGG	+	tig0030153:6595-6614	Msa1442690:CDS	55.0%
!	TCTAAGGGGGGCTCTGATGG+TGG	-	tig0030153:6362-6381	None:intergenic	60.0%
	CCCCCCTTAGAGCCCATTGA+AGG	+	tig0030153:6371-6390	Msa1442690:CDS	60.0%
!	TCTAAGGGGGGCTCTGATGG+TGG	-	tig0030153:6362-6381	None:intergenic	60.0%
	CCCCCCTTAGAGCCCATTGA+AGG	+	tig0030153:6371-6390	Msa1442690:CDS	60.0%
!!	GGCTCTAAGGGGGGCTCTGA+TGG	-	tig0030153:6365-6384	None:intergenic	65.0%
	CCAGGGTGGGATGCAAGGCA+CGG	+	tig0030153:6600-6619	Msa1442690:CDS	65.0%
!!	GGCTCTAAGGGGGGCTCTGA+TGG	-	tig0030153:6365-6384	None:intergenic	65.0%
	CCAGGGTGGGATGCAAGGCA+CGG	+	tig0030153:6600-6619	Msa1442690:CDS	65.0%
	CCGTGCCTTGCATCCCACCC+TGG	-	tig0030153:6603-6622	None:intergenic	70.0%
	CCGTGCCTTGCATCCCACCC+TGG	-	tig0030153:6603-6622	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
tig0030153	gene	6257	8290	6257	ID=Msa1442690;Name=Msa1442690
tig0030153	mRNA	6257	8290	6257	ID=Msa1442690-mRNA-1;Parent=Msa1442690;Name=Msa1442690-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|677
tig0030153	exon	6257	8290	6257	ID=Msa1442690-mRNA-1:exon:13477;Parent=Msa1442690-mRNA-1
tig0030153	CDS	6257	8290	6257	ID=Msa1442690-mRNA-1:cds;Parent=Msa1442690-mRNA-1

Gene Sequence

>Msa1442690

ATGGCAGGTCATGAGATGACAGATATGGATGGAGATTTTACCATGGTTGTCTCAAATGATACACATGTCAGTGCTCTTGATGTGAAGCCTCTCAATAGTGAACCACCATCAGAGCCCCCCTTAGAGCCCATTGAAGGAATGGAGTTTAAATCATTTGAAGAAGCAAAGAGTTACTACACAAGGTACGCTCAGAACAAAGGTTTTAGTTTCCGCATGGGCCGTGTTACTAAGTCAAGAACAAACGGTATGATAATTGGTCAAGAAATTCTTTGTTCCAAGGAGGGGTTTCGAGCCAAAAAGTATGTGAAACAAGGAAATAGTAGTCTTATTACACATGATGAAACCAGGGTGGGATGCAAGGCACGGTTATATTTAAAGAAAAATAATGATATATGGATTGTTTCTAGATTTGTAAGTGACCATAATCACCAGCTGTTTTCTCCTAGAAGTGCACAGTCTCTTCGCGTACATAGAAAGAAAACTAAAGTGCAAAAGACACTTACTGATGTGCTTGATGAATCTGGTTTAGGCAAAACAACATCTGTCTTATGCACTGAAAGTGGCGGTATTGATAATTTTGGATCTAGCCAACAAGATGTTATCAATTACTTAAGTGTACAGAGACAAAAACAATTTGAAAACAGAGATGCTCAATTGATGTTATCATACTTTAAGAATTGTCAGTTGAAGAATACTGGGTTTTTCTATGCGTTTCAAATGGATGCCGAAGGAAAATTAACTAATTGCTTTTGGGTTGATTCAAGGTCTAGAGTTGCTTACAAATATTTTGGTGATGTGGTTACTTTTGATCCAACATACTTGAAAAATAAATACAAAACTCCTTTTGTTCCAATTACTGGAGTTAATCATCATCAACAATCAATTCTTTTTGGTTGTGCTCTCTTGTGGGATGAAACTGTAGAGAGTTTTGTTTGGTTGCTAAGTACTTGGTTAGAAGCAATGAATGGGGTTTGCCCTAAAACTATTATCACAGACCAACACACTGCTATCACCAATGCAGTTGCAAGGGTGTTTCCAAAGGTTAATCACCATTATTGCATGTGGCATATTGAGGAAAAAGTTCCAGAGCACATGAATCAGATCTATCATGGACACAGTGAATTTAAAAATCATTTTTACAAGTGCATTCACCAATCTATCACAATTGAAGAATTTGAGTCTGAATGGGAAGCAATGATTGATAAATACGGGTTGCAAGATAATCAGTGGCTAGTGAAGATATACTCAATTCGATCAAAATGGATTCCTGCATATGTACATCATAATTTTTGTGCTGGAATGTCTACCACTCATAGGAGTGAAAGTATGAATAAGTTTTTTAAGGATTTCTTGAATTCAAGTACTCCATTGAGCAAGTTTCTGACACAATATGAGAAAGCTCTTGATGCACGTTACAACGAGGAAAGGGAGAAAACTGTCAAGACGATGAATTCAAAACCACTTTTGCGAACTTTATACCCCATGGAAGAAGAAGCTTCAAAAATTTATACAGGAAAATTGTTTGAGATATTTCAAGATGAGTTGGTTGGCTCTCAAATGTTTACCACAGAAAAAGTTGAATTTTCTGATGAAGTGGCAACATACAAAGTTCATGAAATTTACAAAGAGAAGCCTAAATATCATGTGACTTTTCATGTAACTTCAAAAGAAGCAACTTGTACTTGTCAAAAGTTTGAATCGTTTGGTATTCTTTGTAGGCATATCTTAACTGTGTTCCTAAAGAAAAAGGTACGTTATCTCCCTTCACAGTATGTTCTACAACGATGGACCCGAAATGCTAAGAAAGAAAAATTTGAAGGATTGACAATTGAAGAATTTGAAGGAGGAAACAAAGCTTCTAGCACTTCATTGTTTAATAGTGTTATGGTTCGTTCACTGGAATTTTCTGAAAGAGCTTCATGGTCAAAAAAACATCATGATATTGCAATTCAATGTTTGCAAAATGCAATTTCAAAACTTGATCTATTAGAGCTTGAAGAATCAAATGAGGAGTTTGTTAATTTGACATCTTAA

Protein sequence

>Msa1442690

MAGHEMTDMDGDFTMVVSNDTHVSALDVKPLNSEPPSEPPLEPIEGMEFKSFEEAKSYYTRYAQNKGFSFRMGRVTKSRTNGMIIGQEILCSKEGFRAKKYVKQGNSSLITHDETRVGCKARLYLKKNNDIWIVSRFVSDHNHQLFSPRSAQSLRVHRKKTKVQKTLTDVLDESGLGKTTSVLCTESGGIDNFGSSQQDVINYLSVQRQKQFENRDAQLMLSYFKNCQLKNTGFFYAFQMDAEGKLTNCFWVDSRSRVAYKYFGDVVTFDPTYLKNKYKTPFVPITGVNHHQQSILFGCALLWDETVESFVWLLSTWLEAMNGVCPKTIITDQHTAITNAVARVFPKVNHHYCMWHIEEKVPEHMNQIYHGHSEFKNHFYKCIHQSITIEEFESEWEAMIDKYGLQDNQWLVKIYSIRSKWIPAYVHHNFCAGMSTTHRSESMNKFFKDFLNSSTPLSKFLTQYEKALDARYNEEREKTVKTMNSKPLLRTLYPMEEEASKIYTGKLFEIFQDELVGSQMFTTEKVEFSDEVATYKVHEIYKEKPKYHVTFHVTSKEATCTCQKFESFGILCRHILTVFLKKKVRYLPSQYVLQRWTRNAKKEKFEGLTIEEFEGGNKASSTSLFNSVMVRSLEFSERASWSKKHHDIAIQCLQNAISKLDLLELEESNEEFVNLTS*