AlfalfaGEDB | Msa0132760

M. sativa cultivar zhongmu-4 / Msa0132760

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF03101.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0132760	sp\|Q9SZL8\|FRS5_ARATH	41.094	640	368	8	41	674	71	707	2.91e-165	496

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0132760	A0A396JW00	96.593	675	22	1	4	677	12	686	0.0	1371

TFs/TRs:

Gene ID	Type	Classification
Msa0132760	TF	FAR1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0036140	Msa0132760	0.803124	4.048998e-49	-8.615850e-47
Msa0058600	Msa0132760	0.801698	7.962942e-49	-8.615850e-47
Msa0083460	Msa0132760	0.875533	2.859869e-68	-8.615850e-47
Msa0132760	Msa0274670	0.801019	1.096707e-48	-8.615850e-47
Msa0132760	Msa0484780	0.806667	7.359576e-50	-8.615850e-47
Msa0132760	Msa0512870	0.811181	7.959350e-51	-8.615850e-47
Msa0132760	Msa0579100	0.809202	2.125945e-50	-8.615850e-47
Msa0132760	Msa0579130	0.813893	2.032071e-51	-8.615850e-47
Msa0132760	Msa0579200	0.845549	3.789850e-59	-8.615850e-47
Msa0132760	Msa0598160	0.810740	9.918776e-51	-8.615850e-47
Msa0132760	Msa0610670	0.833706	4.571230e-56	-8.615850e-47
Msa0132760	Msa0636020	0.804329	2.275854e-49	-8.615850e-47
Msa0132760	Msa0637900	0.809458	1.873761e-50	-8.615850e-47
Msa0132760	Msa0651270	0.817580	3.063708e-52	-8.615850e-47
Msa0132760	Msa0685750	0.812360	4.409529e-51	-8.615850e-47
Msa0132760	Msa0704920	0.809192	2.136542e-50	-8.615850e-47
Msa0132760	Msa0711790	0.820715	5.923193e-53	-8.615850e-47
Msa0132760	Msa0764720	0.801480	8.829246e-49	-8.615850e-47
Msa0132760	Msa0830530	0.814669	1.369777e-51	-8.615850e-47
Msa0132760	Msa0900620	0.801060	1.076099e-48	-8.615850e-47
Msa0132760	Msa1297290	0.801706	7.934563e-49	-8.615850e-47
Msa0132760	Msa1385000	0.800021	1.752442e-48	-8.615850e-47
Msa0132760	Msa1410210	0.811755	5.972906e-51	-8.615850e-47
Msa0132760	Msa1424680	0.812096	5.034015e-51	-8.615850e-47
Msa0132760	Msa1441630	0.844851	5.851060e-59	-8.615850e-47
Msa0132760	Msa1442690	0.914956	1.041804e-84	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0132760	MtrunA17_Chr1g0202341	96.593	675	22	1	4	677	12	686	0.0	1371
Msa0132760	MtrunA17_Chr5g0448761	41.444	637	356	7	36	660	53	684	2.39e-170	506
Msa0132760	MtrunA17_Chr1g0207551	34.335	632	401	6	41	669	32	652	6.03e-122	382
Msa0132760	MtrunA17_Chr5g0440451	34.472	644	381	9	42	664	42	665	9.26e-122	385
Msa0132760	MtrunA17_Chr5g0448511	38.612	461	282	1	204	664	1	460	1.25e-117	363
Msa0132760	MtrunA17_Chr2g0324011	33.777	601	374	10	37	625	4	592	3.87e-116	360
Msa0132760	MtrunA17_Chr2g0296781	37.478	571	328	13	47	602	1	557	7.21e-116	364
Msa0132760	MtrunA17_Chr5g0441581	34.380	605	367	13	9	603	1	585	1.64e-115	362
Msa0132760	MtrunA17_Chr2g0329031	33.601	622	389	9	42	659	13	614	2.94e-115	362
Msa0132760	MtrunA17_Chr3g0080351	33.182	660	393	11	16	656	43	673	4.41e-114	362
Msa0132760	MtrunA17_Chr2g0287061	34.928	627	351	12	41	660	181	757	1.32e-113	360
Msa0132760	MtrunA17_Chr8g0363841	33.442	616	373	13	27	626	5	599	2.75e-112	353
Msa0132760	MtrunA17_Chr3g0118611	38.627	466	278	3	203	664	101	562	1.31e-110	351
Msa0132760	MtrunA17_Chr2g0291581	31.204	673	427	11	5	664	34	683	7.91e-109	344
Msa0132760	MtrunA17_Chr1g0203721	30.903	631	425	6	46	668	66	693	2.52e-102	332
Msa0132760	MtrunA17_Chr6g0458391	31.972	563	357	6	43	601	225	765	4.41e-101	326
Msa0132760	MtrunA17_Chr6g0458391	30.675	163	97	5	23	177	76	230	1.94e-12	70.5
Msa0132760	MtrunA17_Chr2g0287651	31.690	568	375	7	42	603	74	634	8.67e-101	328
Msa0132760	MtrunA17_Chr6g0477261	38.182	440	261	6	172	602	9	446	1.22e-100	322
Msa0132760	MtrunA17_Chr3g0082121	32.154	622	401	7	26	642	26	631	1.24e-97	315
Msa0132760	MtrunA17_Chr1g0196541	33.451	568	355	9	47	602	1	557	4.73e-97	314
Msa0132760	MtrunA17_Chr2g0291591	33.268	511	323	8	42	545	63	562	6.78e-91	293
Msa0132760	MtrunA17_Chr1g0203051	29.021	572	388	8	24	589	63	622	1.56e-90	295
Msa0132760	MtrunA17_Chr1g0198761	29.936	628	410	13	45	654	49	664	6.84e-84	280
Msa0132760	MtrunA17_Chr2g0276841	28.413	630	421	13	43	660	70	681	2.20e-74	253
Msa0132760	MtrunA17_Chr2g0290391	30.208	576	370	11	41	598	49	610	3.81e-70	241
Msa0132760	MtrunA17_Chr2g0294841	26.977	645	434	13	19	638	14	646	7.12e-69	239
Msa0132760	MtrunA17_Chr1g0164591	37.171	304	180	3	134	435	14	308	3.91e-63	213
Msa0132760	MtrunA17_Chr5g0431001	29.825	456	297	10	19	455	33	484	2.99e-57	202
Msa0132760	MtrunA17_Chr7g0258861	36.713	286	174	5	42	322	9	292	8.45e-56	192
Msa0132760	MtrunA17_Chr4g0052331	55.789	95	42	0	1	95	1	95	7.93e-28	107
Msa0132760	MtrunA17_Chr3g0077241	60.870	92	32	2	6	96	1	89	1.15e-27	108
Msa0132760	MtrunA17_Chr2g0329361	44.954	109	59	1	41	148	59	167	2.35e-24	102
Msa0132760	MtrunA17_Chr2g0308841	27.232	224	156	3	381	600	6	226	6.88e-24	101
Msa0132760	MtrunA17_Chr2g0329421	38.596	114	58	3	42	150	26	132	5.68e-19	86.3
Msa0132760	MtrunA17_Chr4g0033611	33.582	134	77	3	17	145	50	176	6.55e-18	84.0
Msa0132760	MtrunA17_Chr1g0149131	32.727	110	74	0	321	430	1	110	2.35e-16	75.9
Msa0132760	MtrunA17_Chr3g0078041	32.738	168	107	5	7	170	12	177	7.77e-16	77.4
Msa0132760	MtrunA17_Chr7g0233911	32.812	128	82	3	42	168	33	157	6.12e-13	68.6
Msa0132760	MtrunA17_Chr6g0451441	26.794	209	139	6	3	208	50	247	8.49e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0132760	AT4G38180.1	41.094	640	368	8	41	674	71	707	2.95e-166	496
Msa0132760	AT4G15090.1	35.275	618	385	8	41	653	49	656	2.39e-127	397
Msa0132760	AT4G15090.5	35.275	618	385	8	41	653	49	656	2.39e-127	397
Msa0132760	AT4G15090.3	35.275	618	385	8	41	653	49	656	2.39e-127	397
Msa0132760	AT4G15090.2	35.275	618	385	8	41	653	49	656	2.39e-127	397
Msa0132760	AT4G15090.4	35.275	618	385	8	41	653	49	656	2.39e-127	397
Msa0132760	AT5G18960.2	38.078	562	303	9	42	601	210	728	5.98e-125	389
Msa0132760	AT5G18960.2	29.078	141	67	4	41	163	41	166	1.75e-12	71.2
Msa0132760	AT5G18960.1	38.078	562	303	9	42	601	210	728	5.98e-125	389
Msa0132760	AT5G18960.1	29.078	141	67	4	41	163	41	166	1.75e-12	71.2
Msa0132760	AT3G06250.2	36.649	573	314	8	32	601	178	704	7.03e-124	386
Msa0132760	AT3G06250.2	32.456	114	61	2	33	145	18	116	3.75e-13	73.6
Msa0132760	AT3G06250.1	36.649	573	314	8	32	601	178	704	7.03e-124	386
Msa0132760	AT3G06250.1	32.456	114	61	2	33	145	18	116	3.75e-13	73.6
Msa0132760	AT3G06250.3	36.649	573	314	8	32	601	178	704	7.03e-124	386
Msa0132760	AT3G06250.3	32.456	114	61	2	33	145	18	116	3.75e-13	73.6
Msa0132760	AT2G27110.2	34.189	623	377	8	42	663	48	638	1.63e-122	385
Msa0132760	AT2G27110.1	34.189	623	377	8	42	663	48	638	1.63e-122	385
Msa0132760	AT3G22170.2	31.379	631	391	9	41	658	68	669	6.52e-113	359
Msa0132760	AT3G22170.1	31.379	631	391	9	41	658	68	669	6.52e-113	359
Msa0132760	AT2G27110.3	38.667	450	269	3	215	663	50	493	4.86e-111	351
Msa0132760	AT1G76320.3	31.942	623	388	9	47	659	1	597	6.74e-103	328
Msa0132760	AT1G76320.1	31.942	623	388	9	47	659	1	597	2.56e-102	328
Msa0132760	AT1G76320.4	31.942	623	388	9	47	659	1	597	2.56e-102	328
Msa0132760	AT1G76320.2	32.149	619	392	10	47	659	1	597	2.95e-102	328
Msa0132760	AT1G52520.1	30.236	635	410	11	43	664	85	699	1.27e-100	323
Msa0132760	AT4G38170.1	35.973	442	276	3	220	660	21	456	1.17e-97	311
Msa0132760	AT2G32250.5	31.495	562	338	6	46	604	41	558	3.83e-94	308
Msa0132760	AT2G32250.2	31.673	562	337	6	46	604	41	558	6.46e-94	308
Msa0132760	AT2G32250.4	31.673	562	337	6	46	604	41	558	6.46e-94	308
Msa0132760	AT2G32250.6	31.673	562	337	6	46	604	41	558	7.70e-94	308
Msa0132760	AT2G32250.1	31.673	562	337	6	46	604	41	558	7.70e-94	308
Msa0132760	AT2G32250.3	31.673	562	337	6	46	604	41	558	7.70e-94	308
Msa0132760	AT4G19990.3	30.612	637	382	9	41	659	19	613	1.14e-93	306
Msa0132760	AT4G19990.4	30.612	637	382	9	41	659	19	613	1.14e-93	306
Msa0132760	AT4G19990.5	30.568	651	387	10	41	668	19	627	1.58e-93	306
Msa0132760	AT4G19990.7	30.612	637	382	9	41	659	33	627	1.89e-93	306
Msa0132760	AT4G19990.6	30.568	651	387	10	41	668	19	627	3.04e-93	306
Msa0132760	AT4G19990.2	30.568	651	387	10	41	668	19	627	3.04e-93	306
Msa0132760	AT2G32250.8	31.373	561	338	6	47	604	1	517	4.36e-93	305
Msa0132760	AT2G32250.7	31.373	561	338	6	47	604	1	517	4.64e-93	305
Msa0132760	AT1G80010.1	29.787	658	399	16	39	663	93	720	9.44e-93	303
Msa0132760	AT4G19990.1	30.651	584	354	9	94	668	29	570	5.58e-85	282
Msa0132760	AT5G28530.1	27.488	633	427	12	43	655	55	675	1.09e-73	251
Msa0132760	AT5G28530.3	27.172	633	425	12	43	655	55	671	1.91e-72	248
Msa0132760	AT5G28530.2	27.172	633	425	12	43	655	55	671	1.91e-72	248
Msa0132760	AT1G10240.2	27.670	618	408	14	6	598	1	604	2.19e-71	245
Msa0132760	AT1G10240.1	27.670	618	408	14	6	598	1	604	2.19e-71	245
Msa0132760	AT5G28530.4	27.520	367	260	3	289	655	252	612	2.63e-45	171
Msa0132760	AT5G28530.5	26.975	367	258	3	289	655	252	608	3.53e-44	168
Msa0132760	AT3G59470.3	38.053	113	58	3	42	149	69	174	2.58e-18	85.5
Msa0132760	AT3G59470.1	38.053	113	58	3	42	149	69	174	2.58e-18	85.5
Msa0132760	AT3G59470.2	38.053	113	58	3	42	149	81	186	3.46e-18	85.5
Msa0132760	AT3G07500.1	32.298	161	83	4	37	173	27	185	1.09e-14	74.3
Msa0132760	AT4G12850.4	34.286	105	63	2	42	145	10	109	1.95e-13	68.6
Msa0132760	AT2G43280.1	34.959	123	77	3	32	152	11	132	2.27e-13	70.1
Msa0132760	AT4G12850.2	34.286	105	63	2	42	145	10	109	1.11e-12	66.2
Msa0132760	AT4G12850.1	34.286	105	63	2	42	145	10	109	1.55e-12	67.0

Find 94 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTGGCGGTATTGATAATTT+TGG	0.195979	1_3:-64036087	Msa0132760:CDS
GAAAATTAACTAATTGCTTT+TGG	0.203998	1_3:-64035915	Msa0132760:CDS
CATTCCTTCAATGGGCTCTA+AGG	0.236305	1_3:+64036527	None:intergenic
TGATGATTAACTCCAGTAAT+TGG	0.238736	1_3:+64035796	None:intergenic
CGAATAGACTGTGCACTTCT+AGG	0.252402	1_3:+64036204	None:intergenic
TGAAAAGTCACATGATATTT+AGG	0.266210	1_3:+64035013	None:intergenic
AGAGTTGCTTACAAATATTT+TGG	0.269630	1_3:-64035877	Msa0132760:CDS
AATTGTCAGTTGAAGAATAC+TGG	0.271394	1_3:-64035970	Msa0132760:CDS
AACTGTGTTCCTAAAGAAAA+AGG	0.312218	1_3:-64034918	Msa0132760:CDS
AATCGTTTGGTATTCTTTGT+AGG	0.315386	1_3:-64034949	Msa0132760:CDS
TATACTCAATTCGATCAAAA+TGG	0.317025	1_3:-64035405	Msa0132760:CDS
CAATATGCCACATGCAATAA+TGG	0.325491	1_3:+64035596	None:intergenic
TTCATTGTTTAATAGTGTTA+TGG	0.327096	1_3:-64034780	Msa0132760:CDS
ATTCTTCAATTGTGATAGAT+TGG	0.351090	1_3:+64035494	None:intergenic
ACTGATGTGCTTGATGAATC+TGG	0.354525	1_3:-64036144	Msa0132760:CDS
GAGAAAACAGCTGGTGATTA+TGG	0.357013	1_3:+64036226	None:intergenic
TGTCAAAAGTTTGAATCGTT+TGG	0.358658	1_3:-64034962	Msa0132760:CDS
TTTAAACTCCATTCCTTCAA+TGG	0.364249	1_3:+64036518	None:intergenic
AGAACAAACGGTATGATAAT+TGG	0.370543	1_3:-64036411	Msa0132760:CDS
TAGTGTTATGGTTCGTTCAC+TGG	0.382958	1_3:-64034768	Msa0132760:CDS
ATTCCTTCAATGGGCTCTAA+GGG	0.384475	1_3:+64036528	None:intergenic
CAATAATGGTGATTAACCTT+TGG	0.392421	1_3:+64035610	None:intergenic
TTGAAGAATTTGAGTCTGAA+TGG	0.400537	1_3:-64035480	Msa0132760:CDS
AGTAATTGATAACATCTTGT+TGG	0.416145	1_3:+64036058	None:intergenic
TTAAACTCCATTCCTTCAAT+GGG	0.435121	1_3:+64036519	None:intergenic
GTGCTTGATGAATCTGGTTT+AGG	0.447135	1_3:-64036138	Msa0132760:CDS
TTGACAATTGAAGAATTTGA+AGG	0.456352	1_3:-64034824	Msa0132760:CDS
CTTGGTTAGAAGCAATGAAT+GGG	0.462263	1_3:-64035699	Msa0132760:CDS
TTGTTTGGTTGCTAAGTACT+TGG	0.462493	1_3:-64035717	Msa0132760:CDS
TGAAGAATTTGAGTCTGAAT+GGG	0.463681	1_3:-64035479	Msa0132760:CDS
TCCTTCAATGGGCTCTAAGG+GGG	0.466966	1_3:+64036530	None:intergenic
TAGATCTGATTCATGTGCTC+TGG	0.470489	1_3:+64035562	None:intergenic
CTGGAATGTCTACCACTCAT+AGG	0.472850	1_3:-64035351	Msa0132760:CDS
TTTGGCTCGAAACCCCTCCT+TGG	0.475441	1_3:+64036371	None:intergenic
ACTCCAGTAATTGGAACAAA+AGG	0.475504	1_3:+64035805	None:intergenic
TTTGAAGCTTCTTCTTCCAT+GGG	0.477189	1_3:+64035166	None:intergenic
ACTGTTTGCGTTATTATGGC+AGG	0.480651	1_3:-64036660	None:intergenic
TTATTGCATGTGGCATATTG+AGG	0.485948	1_3:-64035593	Msa0132760:CDS
ACTTGGTTAGAAGCAATGAA+TGG	0.491300	1_3:-64035700	Msa0132760:CDS
CACATGAATCAGATCTATCA+TGG	0.493856	1_3:-64035556	Msa0132760:CDS
GAAGCAATGATTGATAAATA+CGG	0.502447	1_3:-64035457	Msa0132760:CDS
AAATTATGATGTACATATGC+AGG	0.504291	1_3:+64035379	None:intergenic
GGGTATAAAGTTCGCAAAAG+TGG	0.509142	1_3:+64035187	None:intergenic
TCAAGAAATTCTTTGTTCCA+AGG	0.511520	1_3:-64036388	Msa0132760:CDS
CCTTCAATGGGCTCTAAGGG+GGG	0.511902	1_3:+64036531	None:intergenic
AAGCAATGATTGATAAATAC+GGG	0.513517	1_3:-64035456	Msa0132760:CDS
CACAGTATGTTCTACAACGA+TGG	0.518628	1_3:-64034880	Msa0132760:CDS
TGTGTCAGAAACTTGCTCAA+TGG	0.523310	1_3:+64035280	None:intergenic
CTTAGAGCCCATTGAAGGAA+TGG	0.526994	1_3:-64036526	Msa0132760:CDS
ACAATTGAAGAATTTGAAGG+AGG	0.530417	1_3:-64034821	Msa0132760:CDS
CACTTCTAGGAGAAAACAGC+TGG	0.530595	1_3:+64036217	None:intergenic
TGAGATATTTCAAGATGAGT+TGG	0.531340	1_3:-64035122	Msa0132760:CDS
GTTACTAAGTCAAGAACAAA+CGG	0.531428	1_3:-64036423	Msa0132760:CDS
AAATTCTTTGTTCCAAGGAG+GGG	0.533751	1_3:-64036383	Msa0132760:CDS
AGGTCATGAGATGACAGATA+TGG	0.541561	1_3:-64036640	Msa0132760:CDS
GATGGTGGTTCACTATTGAG+AGG	0.542446	1_3:+64036558	None:intergenic
ATATTTCAAGATGAGTTGGT+TGG	0.542621	1_3:-64035118	Msa0132760:CDS
ATTGTCAGTTGAAGAATACT+GGG	0.543680	1_3:-64035969	Msa0132760:CDS
CTATCACCAATGCAGTTGCA+AGG	0.546795	1_3:-64035639	Msa0132760:CDS
GAAATTCTTTGTTCCAAGGA+GGG	0.547884	1_3:-64036384	Msa0132760:CDS
TGCACGTTACAACGAGGAAA+GGG	0.548977	1_3:-64035239	Msa0132760:CDS
TGAAACCAGGGTGGGATGCA+AGG	0.555494	1_3:-64036307	Msa0132760:CDS
AAACACCCTTGCAACTGCAT+TGG	0.561204	1_3:+64035633	None:intergenic
TCTGTCTTATGCACTGAAAG+TGG	0.562246	1_3:-64036105	Msa0132760:CDS
TTCCTTCAATGGGCTCTAAG+GGG	0.577393	1_3:+64036529	None:intergenic
TTAATCACCATTATTGCATG+TGG	0.578974	1_3:-64035603	Msa0132760:CDS
CCGTGCCTTGCATCCCACCC+TGG	0.580963	1_3:+64036302	None:intergenic
TTTGCGAACTTTATACCCCA+TGG	0.583317	1_3:-64035182	Msa0132760:CDS
AGTTGCAAGGGTGTTTCCAA+AGG	0.585018	1_3:-64035626	Msa0132760:CDS
ATGCACGTTACAACGAGGAA+AGG	0.585794	1_3:-64035240	Msa0132760:CDS
CGTTGTAGAACATACTGTGA+AGG	0.588365	1_3:+64034884	None:intergenic
CATGAGATGACAGATATGGA+TGG	0.601625	1_3:-64036636	Msa0132760:CDS
AGAAATTCTTTGTTCCAAGG+AGG	0.606491	1_3:-64036385	Msa0132760:CDS
TTGAAGCTTCTTCTTCCATG+GGG	0.606500	1_3:+64035167	None:intergenic
CATTGGTGATAGCAGTGTGT+TGG	0.606939	1_3:+64035650	None:intergenic
GTTGTAGAACATACTGTGAA+GGG	0.621697	1_3:+64034885	None:intergenic
GCGTTTCAAATGGATGCCGA+AGG	0.622605	1_3:-64035937	Msa0132760:CDS
AGAGCTTGAAGAATCAAATG+AGG	0.626048	1_3:-64034657	Msa0132760:CDS
TATCACCAATGCAGTTGCAA+GGG	0.633978	1_3:-64035638	Msa0132760:CDS
TTATTACACATGATGAAACC+AGG	0.637175	1_3:-64036320	Msa0132760:CDS
CATACTTTCACTCCTATGAG+TGG	0.639566	1_3:+64035339	None:intergenic
ACAAGGTACGCTCAGAACAA+AGG	0.639870	1_3:-64036468	Msa0132760:CDS
CCAGGGTGGGATGCAAGGCA+CGG	0.641442	1_3:-64036302	Msa0132760:CDS
ACGGGTTGCAAGATAATCAG+TGG	0.648204	1_3:-64035438	Msa0132760:CDS
TTGGTTAGAAGCAATGAATG+GGG	0.648562	1_3:-64035698	Msa0132760:CDS
AAGCAAAGAGTTACTACACA+AGG	0.651539	1_3:-64036485	Msa0132760:CDS
ACACATGATGAAACCAGGGT+GGG	0.653979	1_3:-64036315	Msa0132760:CDS
ACTTAGTAACACGGCCCATG+CGG	0.656322	1_3:+64036436	None:intergenic
GTCTTATGCACTGAAAGTGG+CGG	0.656902	1_3:-64036102	Msa0132760:CDS
TTGTTCTTGACTTAGTAACA+CGG	0.665948	1_3:+64036427	None:intergenic
TACACATGATGAAACCAGGG+TGG	0.688016	1_3:-64036316	Msa0132760:CDS
TATTACACATGATGAAACCA+GGG	0.694710	1_3:-64036319	Msa0132760:CDS
TGTATCATTTGAGACAACCA+TGG	0.701765	1_3:+64036605	None:intergenic
TCTTGATGCACGTTACAACG+AGG	0.755842	1_3:-64035245	Msa0132760:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAGAAAAATAATGATATA+TGG	-	chr1_3:64035007-64035026	Msa0132760:CDS	10.0%
!!	TAAAGAAAAATAATGATATA+TGG	-	chr1_3:64035007-64035026	Msa0132760:CDS	10.0%
!!!	AAAATTAACTAATTGCTTTT+GGG	-	chr1_3:64035365-64035384	Msa0132760:CDS	15.0%
!!!	TATTTATTTTTCAAGTATGT+TGG	+	chr1_3:64035447-64035466	None:intergenic	15.0%
!!!	AAGTATGAATAAGTTTTTTA+AGG	-	chr1_3:64035956-64035975	Msa0132760:CDS	15.0%
!!!	AAAATTAACTAATTGCTTTT+GGG	-	chr1_3:64035365-64035384	Msa0132760:CDS	15.0%
!!!	TATTTATTTTTCAAGTATGT+TGG	+	chr1_3:64035447-64035466	None:intergenic	15.0%
!!!	AAGTATGAATAAGTTTTTTA+AGG	-	chr1_3:64035956-64035975	Msa0132760:CDS	15.0%
!!!	GAAAATTAACTAATTGCTTT+TGG	-	chr1_3:64035364-64035383	Msa0132760:CDS	20.0%
!!!	ATTTTTCTTTCTTAGCATTT+CGG	+	chr1_3:64036427-64036446	None:intergenic	20.0%
!!	TTCATTGTTTAATAGTGTTA+TGG	-	chr1_3:64036499-64036518	Msa0132760:CDS	20.0%
!!!	GAAAATTAACTAATTGCTTT+TGG	-	chr1_3:64035364-64035383	Msa0132760:CDS	20.0%
!!!	ATTTTTCTTTCTTAGCATTT+CGG	+	chr1_3:64036427-64036446	None:intergenic	20.0%
!!	TTCATTGTTTAATAGTGTTA+TGG	-	chr1_3:64036499-64036518	Msa0132760:CDS	20.0%
!!	AGTAATTGATAACATCTTGT+TGG	+	chr1_3:64035224-64035243	None:intergenic	25.0%
!!	GCAATTAGTTAATTTTCCTT+CGG	+	chr1_3:64035361-64035380	None:intergenic	25.0%
!!	AGAGTTGCTTACAAATATTT+TGG	-	chr1_3:64035402-64035421	Msa0132760:CDS	25.0%
!!!	TTACAAATATTTTGGTGATG+TGG	-	chr1_3:64035410-64035429	Msa0132760:CDS	25.0%
!!	CATCAACAATCAATTCTTTT+TGG	-	chr1_3:64035504-64035523	Msa0132760:CDS	25.0%
!	ATTCTTCAATTGTGATAGAT+TGG	+	chr1_3:64035788-64035807	None:intergenic	25.0%
!	GAAGCAATGATTGATAAATA+CGG	-	chr1_3:64035822-64035841	Msa0132760:CDS	25.0%
!	AAGCAATGATTGATAAATAC+GGG	-	chr1_3:64035823-64035842	Msa0132760:CDS	25.0%
!	TATACTCAATTCGATCAAAA+TGG	-	chr1_3:64035874-64035893	Msa0132760:CDS	25.0%
!	AAATTATGATGTACATATGC+AGG	+	chr1_3:64035903-64035922	None:intergenic	25.0%
!	GAAGCTTCAAAAATTTATAC+AGG	-	chr1_3:64036125-64036144	Msa0132760:CDS	25.0%
!!!	AGAAAATTCAACTTTTTCTG+TGG	+	chr1_3:64036201-64036220	None:intergenic	25.0%
!	TGAAAAGTCACATGATATTT+AGG	+	chr1_3:64036269-64036288	None:intergenic	25.0%
!!	TTTTTCTTTCTTAGCATTTC+GGG	+	chr1_3:64036426-64036445	None:intergenic	25.0%
!	GCTAAGAAAGAAAAATTTGA+AGG	-	chr1_3:64036431-64036450	Msa0132760:CDS	25.0%
!	TTGACAATTGAAGAATTTGA+AGG	-	chr1_3:64036455-64036474	Msa0132760:CDS	25.0%
!!	AGTAATTGATAACATCTTGT+TGG	+	chr1_3:64035224-64035243	None:intergenic	25.0%
!!	GCAATTAGTTAATTTTCCTT+CGG	+	chr1_3:64035361-64035380	None:intergenic	25.0%
!!	AGAGTTGCTTACAAATATTT+TGG	-	chr1_3:64035402-64035421	Msa0132760:CDS	25.0%
!!!	TTACAAATATTTTGGTGATG+TGG	-	chr1_3:64035410-64035429	Msa0132760:CDS	25.0%
!!	CATCAACAATCAATTCTTTT+TGG	-	chr1_3:64035504-64035523	Msa0132760:CDS	25.0%
!	ATTCTTCAATTGTGATAGAT+TGG	+	chr1_3:64035788-64035807	None:intergenic	25.0%
!	GAAGCAATGATTGATAAATA+CGG	-	chr1_3:64035822-64035841	Msa0132760:CDS	25.0%
!	AAGCAATGATTGATAAATAC+GGG	-	chr1_3:64035823-64035842	Msa0132760:CDS	25.0%
!	TATACTCAATTCGATCAAAA+TGG	-	chr1_3:64035874-64035893	Msa0132760:CDS	25.0%
!	AAATTATGATGTACATATGC+AGG	+	chr1_3:64035903-64035922	None:intergenic	25.0%
!	GAAGCTTCAAAAATTTATAC+AGG	-	chr1_3:64036125-64036144	Msa0132760:CDS	25.0%
!!!	AGAAAATTCAACTTTTTCTG+TGG	+	chr1_3:64036201-64036220	None:intergenic	25.0%
!	TGAAAAGTCACATGATATTT+AGG	+	chr1_3:64036269-64036288	None:intergenic	25.0%
!!	TTTTTCTTTCTTAGCATTTC+GGG	+	chr1_3:64036426-64036445	None:intergenic	25.0%
!	GCTAAGAAAGAAAAATTTGA+AGG	-	chr1_3:64036431-64036450	Msa0132760:CDS	25.0%
!	TTGACAATTGAAGAATTTGA+AGG	-	chr1_3:64036455-64036474	Msa0132760:CDS	25.0%
	TTAAACTCCATTCCTTCAAT+GGG	+	chr1_3:64034763-64034782	None:intergenic	30.0%
	TTTAAACTCCATTCCTTCAA+TGG	+	chr1_3:64034764-64034783	None:intergenic	30.0%
	TTGTTCTTGACTTAGTAACA+CGG	+	chr1_3:64034855-64034874	None:intergenic	30.0%
	GTTACTAAGTCAAGAACAAA+CGG	-	chr1_3:64034856-64034875	Msa0132760:CDS	30.0%
	AGAACAAACGGTATGATAAT+TGG	-	chr1_3:64034868-64034887	Msa0132760:CDS	30.0%
	TCAAGAAATTCTTTGTTCCA+AGG	-	chr1_3:64034891-64034910	Msa0132760:CDS	30.0%
!!	TCCTTGTTTCACATACTTTT+TGG	+	chr1_3:64034929-64034948	None:intergenic	30.0%
	TTATTACACATGATGAAACC+AGG	-	chr1_3:64034959-64034978	Msa0132760:CDS	30.0%
	TATTACACATGATGAAACCA+GGG	-	chr1_3:64034960-64034979	Msa0132760:CDS	30.0%
	AATTGTCAGTTGAAGAATAC+TGG	-	chr1_3:64035309-64035328	Msa0132760:CDS	30.0%
	ATTGTCAGTTGAAGAATACT+GGG	-	chr1_3:64035310-64035329	Msa0132760:CDS	30.0%
!	GTTTTTCTATGCGTTTCAAA+TGG	-	chr1_3:64035332-64035351	Msa0132760:CDS	30.0%
	TGATGATTAACTCCAGTAAT+TGG	+	chr1_3:64035486-64035505	None:intergenic	30.0%
!!!	AAACTGTAGAGAGTTTTGTT+TGG	-	chr1_3:64035547-64035566	Msa0132760:CDS	30.0%
!!	GGTCTGTGATAATAGTTTTA+GGG	+	chr1_3:64035611-64035630	None:intergenic	30.0%
!	TGGTCTGTGATAATAGTTTT+AGG	+	chr1_3:64035612-64035631	None:intergenic	30.0%
	CAATAATGGTGATTAACCTT+TGG	+	chr1_3:64035672-64035691	None:intergenic	30.0%
	TTAATCACCATTATTGCATG+TGG	-	chr1_3:64035676-64035695	Msa0132760:CDS	30.0%
	TTGAAGAATTTGAGTCTGAA+TGG	-	chr1_3:64035799-64035818	Msa0132760:CDS	30.0%
	TGAAGAATTTGAGTCTGAAT+GGG	-	chr1_3:64035800-64035819	Msa0132760:CDS	30.0%
!	GTACATCATAATTTTTGTGC+TGG	-	chr1_3:64035909-64035928	Msa0132760:CDS	30.0%
	TGAGATATTTCAAGATGAGT+TGG	-	chr1_3:64036157-64036176	Msa0132760:CDS	30.0%
!!	ATATTTCAAGATGAGTTGGT+TGG	-	chr1_3:64036161-64036180	Msa0132760:CDS	30.0%
!	AGTTGAATTTTCTGATGAAG+TGG	-	chr1_3:64036208-64036227	Msa0132760:CDS	30.0%
	TGTCAAAAGTTTGAATCGTT+TGG	-	chr1_3:64036317-64036336	Msa0132760:CDS	30.0%
	AATCGTTTGGTATTCTTTGT+AGG	-	chr1_3:64036330-64036349	Msa0132760:CDS	30.0%
	AACTGTGTTCCTAAAGAAAA+AGG	-	chr1_3:64036361-64036380	Msa0132760:CDS	30.0%
!	GATAACGTACCTTTTTCTTT+AGG	+	chr1_3:64036373-64036392	None:intergenic	30.0%
	ACAATTGAAGAATTTGAAGG+AGG	-	chr1_3:64036458-64036477	Msa0132760:CDS	30.0%
!!	AATTTTCTGAAAGAGCTTCA+TGG	-	chr1_3:64036534-64036553	Msa0132760:CDS	30.0%
	TTAAACTCCATTCCTTCAAT+GGG	+	chr1_3:64034763-64034782	None:intergenic	30.0%
	TTTAAACTCCATTCCTTCAA+TGG	+	chr1_3:64034764-64034783	None:intergenic	30.0%
	TTGTTCTTGACTTAGTAACA+CGG	+	chr1_3:64034855-64034874	None:intergenic	30.0%
	GTTACTAAGTCAAGAACAAA+CGG	-	chr1_3:64034856-64034875	Msa0132760:CDS	30.0%
	AGAACAAACGGTATGATAAT+TGG	-	chr1_3:64034868-64034887	Msa0132760:CDS	30.0%
	TCAAGAAATTCTTTGTTCCA+AGG	-	chr1_3:64034891-64034910	Msa0132760:CDS	30.0%
!!	TCCTTGTTTCACATACTTTT+TGG	+	chr1_3:64034929-64034948	None:intergenic	30.0%
	TTATTACACATGATGAAACC+AGG	-	chr1_3:64034959-64034978	Msa0132760:CDS	30.0%
	TATTACACATGATGAAACCA+GGG	-	chr1_3:64034960-64034979	Msa0132760:CDS	30.0%
	AATTGTCAGTTGAAGAATAC+TGG	-	chr1_3:64035309-64035328	Msa0132760:CDS	30.0%
	ATTGTCAGTTGAAGAATACT+GGG	-	chr1_3:64035310-64035329	Msa0132760:CDS	30.0%
!	GTTTTTCTATGCGTTTCAAA+TGG	-	chr1_3:64035332-64035351	Msa0132760:CDS	30.0%
	TGATGATTAACTCCAGTAAT+TGG	+	chr1_3:64035486-64035505	None:intergenic	30.0%
!!!	AAACTGTAGAGAGTTTTGTT+TGG	-	chr1_3:64035547-64035566	Msa0132760:CDS	30.0%
!!	GGTCTGTGATAATAGTTTTA+GGG	+	chr1_3:64035611-64035630	None:intergenic	30.0%
!	TGGTCTGTGATAATAGTTTT+AGG	+	chr1_3:64035612-64035631	None:intergenic	30.0%
	CAATAATGGTGATTAACCTT+TGG	+	chr1_3:64035672-64035691	None:intergenic	30.0%
	TTAATCACCATTATTGCATG+TGG	-	chr1_3:64035676-64035695	Msa0132760:CDS	30.0%
	TTGAAGAATTTGAGTCTGAA+TGG	-	chr1_3:64035799-64035818	Msa0132760:CDS	30.0%
	TGAAGAATTTGAGTCTGAAT+GGG	-	chr1_3:64035800-64035819	Msa0132760:CDS	30.0%
!	GTACATCATAATTTTTGTGC+TGG	-	chr1_3:64035909-64035928	Msa0132760:CDS	30.0%
	TGAGATATTTCAAGATGAGT+TGG	-	chr1_3:64036157-64036176	Msa0132760:CDS	30.0%
!!	ATATTTCAAGATGAGTTGGT+TGG	-	chr1_3:64036161-64036180	Msa0132760:CDS	30.0%
!	AGTTGAATTTTCTGATGAAG+TGG	-	chr1_3:64036208-64036227	Msa0132760:CDS	30.0%
	TGTCAAAAGTTTGAATCGTT+TGG	-	chr1_3:64036317-64036336	Msa0132760:CDS	30.0%
	AATCGTTTGGTATTCTTTGT+AGG	-	chr1_3:64036330-64036349	Msa0132760:CDS	30.0%
	AACTGTGTTCCTAAAGAAAA+AGG	-	chr1_3:64036361-64036380	Msa0132760:CDS	30.0%
!	GATAACGTACCTTTTTCTTT+AGG	+	chr1_3:64036373-64036392	None:intergenic	30.0%
	ACAATTGAAGAATTTGAAGG+AGG	-	chr1_3:64036458-64036477	Msa0132760:CDS	30.0%
!!	AATTTTCTGAAAGAGCTTCA+TGG	-	chr1_3:64036534-64036553	Msa0132760:CDS	30.0%
!	TATGGATGGAGATTTTACCA+TGG	-	chr1_3:64034657-64034676	Msa0132760:CDS	35.0%
!	TGTATCATTTGAGACAACCA+TGG	+	chr1_3:64034677-64034696	None:intergenic	35.0%
	AAGCAAAGAGTTACTACACA+AGG	-	chr1_3:64034794-64034813	Msa0132760:CDS	35.0%
!!!	AAAGGTTTTAGTTTCCGCAT+GGG	-	chr1_3:64034829-64034848	Msa0132760:CDS	35.0%
	AGAAATTCTTTGTTCCAAGG+AGG	-	chr1_3:64034894-64034913	Msa0132760:CDS	35.0%
	GAAATTCTTTGTTCCAAGGA+GGG	-	chr1_3:64034895-64034914	Msa0132760:CDS	35.0%
	AAATTCTTTGTTCCAAGGAG+GGG	-	chr1_3:64034896-64034915	Msa0132760:CDS	35.0%
!	GCCAAAAAGTATGTGAAACA+AGG	-	chr1_3:64034925-64034944	Msa0132760:CDS	35.0%
!!!	AGTGGCGGTATTGATAATTT+TGG	-	chr1_3:64035192-64035211	Msa0132760:CDS	35.0%
!!!	ATTGCTTTTGGGTTGATTCA+AGG	-	chr1_3:64035376-64035395	Msa0132760:CDS	35.0%
!	ACTCCTTTTGTTCCAATTAC+TGG	-	chr1_3:64035471-64035490	Msa0132760:CDS	35.0%
	ACTCCAGTAATTGGAACAAA+AGG	+	chr1_3:64035477-64035496	None:intergenic	35.0%
	TTGTTTGGTTGCTAAGTACT+TGG	-	chr1_3:64035562-64035581	Msa0132760:CDS	35.0%
	ACTTGGTTAGAAGCAATGAA+TGG	-	chr1_3:64035579-64035598	Msa0132760:CDS	35.0%
	CTTGGTTAGAAGCAATGAAT+GGG	-	chr1_3:64035580-64035599	Msa0132760:CDS	35.0%
	TTGGTTAGAAGCAATGAATG+GGG	-	chr1_3:64035581-64035600	Msa0132760:CDS	35.0%
	CAATATGCCACATGCAATAA+TGG	+	chr1_3:64035686-64035705	None:intergenic	35.0%
	TTATTGCATGTGGCATATTG+AGG	-	chr1_3:64035686-64035705	Msa0132760:CDS	35.0%
	CACATGAATCAGATCTATCA+TGG	-	chr1_3:64035723-64035742	Msa0132760:CDS	35.0%
!	TTTGAAGCTTCTTCTTCCAT+GGG	+	chr1_3:64036116-64036135	None:intergenic	35.0%
!!!	TTTTGAAGCTTCTTCTTCCA+TGG	+	chr1_3:64036117-64036136	None:intergenic	35.0%
	GTTGTAGAACATACTGTGAA+GGG	+	chr1_3:64036397-64036416	None:intergenic	35.0%
	AGAGCTTGAAGAATCAAATG+AGG	-	chr1_3:64036622-64036641	Msa0132760:CDS	35.0%
!	TATGGATGGAGATTTTACCA+TGG	-	chr1_3:64034657-64034676	Msa0132760:CDS	35.0%
!	TGTATCATTTGAGACAACCA+TGG	+	chr1_3:64034677-64034696	None:intergenic	35.0%
	AAGCAAAGAGTTACTACACA+AGG	-	chr1_3:64034794-64034813	Msa0132760:CDS	35.0%
!!!	AAAGGTTTTAGTTTCCGCAT+GGG	-	chr1_3:64034829-64034848	Msa0132760:CDS	35.0%
	AGAAATTCTTTGTTCCAAGG+AGG	-	chr1_3:64034894-64034913	Msa0132760:CDS	35.0%
	GAAATTCTTTGTTCCAAGGA+GGG	-	chr1_3:64034895-64034914	Msa0132760:CDS	35.0%
	AAATTCTTTGTTCCAAGGAG+GGG	-	chr1_3:64034896-64034915	Msa0132760:CDS	35.0%
!	GCCAAAAAGTATGTGAAACA+AGG	-	chr1_3:64034925-64034944	Msa0132760:CDS	35.0%
!!!	AGTGGCGGTATTGATAATTT+TGG	-	chr1_3:64035192-64035211	Msa0132760:CDS	35.0%
!!!	ATTGCTTTTGGGTTGATTCA+AGG	-	chr1_3:64035376-64035395	Msa0132760:CDS	35.0%
!	ACTCCTTTTGTTCCAATTAC+TGG	-	chr1_3:64035471-64035490	Msa0132760:CDS	35.0%
	ACTCCAGTAATTGGAACAAA+AGG	+	chr1_3:64035477-64035496	None:intergenic	35.0%
	TTGTTTGGTTGCTAAGTACT+TGG	-	chr1_3:64035562-64035581	Msa0132760:CDS	35.0%
	ACTTGGTTAGAAGCAATGAA+TGG	-	chr1_3:64035579-64035598	Msa0132760:CDS	35.0%
	CTTGGTTAGAAGCAATGAAT+GGG	-	chr1_3:64035580-64035599	Msa0132760:CDS	35.0%
	TTGGTTAGAAGCAATGAATG+GGG	-	chr1_3:64035581-64035600	Msa0132760:CDS	35.0%
	CAATATGCCACATGCAATAA+TGG	+	chr1_3:64035686-64035705	None:intergenic	35.0%
	TTATTGCATGTGGCATATTG+AGG	-	chr1_3:64035686-64035705	Msa0132760:CDS	35.0%
	CACATGAATCAGATCTATCA+TGG	-	chr1_3:64035723-64035742	Msa0132760:CDS	35.0%
!	TTTGAAGCTTCTTCTTCCAT+GGG	+	chr1_3:64036116-64036135	None:intergenic	35.0%
!!!	TTTTGAAGCTTCTTCTTCCA+TGG	+	chr1_3:64036117-64036136	None:intergenic	35.0%
	GTTGTAGAACATACTGTGAA+GGG	+	chr1_3:64036397-64036416	None:intergenic	35.0%
	AGAGCTTGAAGAATCAAATG+AGG	-	chr1_3:64036622-64036641	Msa0132760:CDS	35.0%
	AGGTCATGAGATGACAGATA+TGG	-	chr1_3:64034639-64034658	Msa0132760:CDS	40.0%
	CATGAGATGACAGATATGGA+TGG	-	chr1_3:64034643-64034662	Msa0132760:CDS	40.0%
!	ATTCCTTCAATGGGCTCTAA+GGG	+	chr1_3:64034754-64034773	None:intergenic	40.0%
!!!	CAAAGGTTTTAGTTTCCGCA+TGG	-	chr1_3:64034828-64034847	Msa0132760:CDS	40.0%
	GAGAAAACAGCTGGTGATTA+TGG	+	chr1_3:64035056-64035075	None:intergenic	40.0%
	ACTGATGTGCTTGATGAATC+TGG	-	chr1_3:64035135-64035154	Msa0132760:CDS	40.0%
!	GTGCTTGATGAATCTGGTTT+AGG	-	chr1_3:64035141-64035160	Msa0132760:CDS	40.0%
	TCTGTCTTATGCACTGAAAG+TGG	-	chr1_3:64035174-64035193	Msa0132760:CDS	40.0%
!!!	TTTTTGGTTGTGCTCTCTTG+TGG	-	chr1_3:64035520-64035539	Msa0132760:CDS	40.0%
!!	TTTTGGTTGTGCTCTCTTGT+GGG	-	chr1_3:64035521-64035540	Msa0132760:CDS	40.0%
	TATCACCAATGCAGTTGCAA+GGG	-	chr1_3:64035641-64035660	Msa0132760:CDS	40.0%
	TAGATCTGATTCATGTGCTC+TGG	+	chr1_3:64035720-64035739	None:intergenic	40.0%
	CATACTTTCACTCCTATGAG+TGG	+	chr1_3:64035943-64035962	None:intergenic	40.0%
!	TGTGTCAGAAACTTGCTCAA+TGG	+	chr1_3:64036002-64036021	None:intergenic	40.0%
	GGGTATAAAGTTCGCAAAAG+TGG	+	chr1_3:64036095-64036114	None:intergenic	40.0%
	TTTGCGAACTTTATACCCCA+TGG	-	chr1_3:64036097-64036116	Msa0132760:CDS	40.0%
	TTGAAGCTTCTTCTTCCATG+GGG	+	chr1_3:64036115-64036134	None:intergenic	40.0%
	CGTTGTAGAACATACTGTGA+AGG	+	chr1_3:64036398-64036417	None:intergenic	40.0%
	CACAGTATGTTCTACAACGA+TGG	-	chr1_3:64036399-64036418	Msa0132760:CDS	40.0%
	TAGTGTTATGGTTCGTTCAC+TGG	-	chr1_3:64036511-64036530	Msa0132760:CDS	40.0%
	AGGTCATGAGATGACAGATA+TGG	-	chr1_3:64034639-64034658	Msa0132760:CDS	40.0%
	CATGAGATGACAGATATGGA+TGG	-	chr1_3:64034643-64034662	Msa0132760:CDS	40.0%
!	ATTCCTTCAATGGGCTCTAA+GGG	+	chr1_3:64034754-64034773	None:intergenic	40.0%
!!!	CAAAGGTTTTAGTTTCCGCA+TGG	-	chr1_3:64034828-64034847	Msa0132760:CDS	40.0%
	GAGAAAACAGCTGGTGATTA+TGG	+	chr1_3:64035056-64035075	None:intergenic	40.0%
	ACTGATGTGCTTGATGAATC+TGG	-	chr1_3:64035135-64035154	Msa0132760:CDS	40.0%
!	GTGCTTGATGAATCTGGTTT+AGG	-	chr1_3:64035141-64035160	Msa0132760:CDS	40.0%
	TCTGTCTTATGCACTGAAAG+TGG	-	chr1_3:64035174-64035193	Msa0132760:CDS	40.0%
!!!	TTTTTGGTTGTGCTCTCTTG+TGG	-	chr1_3:64035520-64035539	Msa0132760:CDS	40.0%
!!	TTTTGGTTGTGCTCTCTTGT+GGG	-	chr1_3:64035521-64035540	Msa0132760:CDS	40.0%
	TATCACCAATGCAGTTGCAA+GGG	-	chr1_3:64035641-64035660	Msa0132760:CDS	40.0%
	TAGATCTGATTCATGTGCTC+TGG	+	chr1_3:64035720-64035739	None:intergenic	40.0%
	CATACTTTCACTCCTATGAG+TGG	+	chr1_3:64035943-64035962	None:intergenic	40.0%
!	TGTGTCAGAAACTTGCTCAA+TGG	+	chr1_3:64036002-64036021	None:intergenic	40.0%
	GGGTATAAAGTTCGCAAAAG+TGG	+	chr1_3:64036095-64036114	None:intergenic	40.0%
	TTTGCGAACTTTATACCCCA+TGG	-	chr1_3:64036097-64036116	Msa0132760:CDS	40.0%
	TTGAAGCTTCTTCTTCCATG+GGG	+	chr1_3:64036115-64036134	None:intergenic	40.0%
	CGTTGTAGAACATACTGTGA+AGG	+	chr1_3:64036398-64036417	None:intergenic	40.0%
	CACAGTATGTTCTACAACGA+TGG	-	chr1_3:64036399-64036418	Msa0132760:CDS	40.0%
	TAGTGTTATGGTTCGTTCAC+TGG	-	chr1_3:64036511-64036530	Msa0132760:CDS	40.0%
!	GATGGTGGTTCACTATTGAG+AGG	+	chr1_3:64034724-64034743	None:intergenic	45.0%
!!	TTCCTTCAATGGGCTCTAAG+GGG	+	chr1_3:64034753-64034772	None:intergenic	45.0%
!	CATTCCTTCAATGGGCTCTA+AGG	+	chr1_3:64034755-64034774	None:intergenic	45.0%
	CTTAGAGCCCATTGAAGGAA+TGG	-	chr1_3:64034753-64034772	Msa0132760:CDS	45.0%
	ACAAGGTACGCTCAGAACAA+AGG	-	chr1_3:64034811-64034830	Msa0132760:CDS	45.0%
	TACACATGATGAAACCAGGG+TGG	-	chr1_3:64034963-64034982	Msa0132760:CDS	45.0%
	ACACATGATGAAACCAGGGT+GGG	-	chr1_3:64034964-64034983	Msa0132760:CDS	45.0%
!	CACTTCTAGGAGAAAACAGC+TGG	+	chr1_3:64035065-64035084	None:intergenic	45.0%
	CGAATAGACTGTGCACTTCT+AGG	+	chr1_3:64035078-64035097	None:intergenic	45.0%
	GTCTTATGCACTGAAAGTGG+CGG	-	chr1_3:64035177-64035196	Msa0132760:CDS	45.0%
!	CATTGGTGATAGCAGTGTGT+TGG	+	chr1_3:64035632-64035651	None:intergenic	45.0%
	CTATCACCAATGCAGTTGCA+AGG	-	chr1_3:64035640-64035659	Msa0132760:CDS	45.0%
	AAACACCCTTGCAACTGCAT+TGG	+	chr1_3:64035649-64035668	None:intergenic	45.0%
!!	AGTTGCAAGGGTGTTTCCAA+AGG	-	chr1_3:64035653-64035672	Msa0132760:CDS	45.0%
!	ACGGGTTGCAAGATAATCAG+TGG	-	chr1_3:64035841-64035860	Msa0132760:CDS	45.0%
	CTGGAATGTCTACCACTCAT+AGG	-	chr1_3:64035928-64035947	Msa0132760:CDS	45.0%
	TCTTGATGCACGTTACAACG+AGG	-	chr1_3:64036034-64036053	Msa0132760:CDS	45.0%
	ATGCACGTTACAACGAGGAA+AGG	-	chr1_3:64036039-64036058	Msa0132760:CDS	45.0%
	TGCACGTTACAACGAGGAAA+GGG	-	chr1_3:64036040-64036059	Msa0132760:CDS	45.0%
!	GATGGTGGTTCACTATTGAG+AGG	+	chr1_3:64034724-64034743	None:intergenic	45.0%
!!	TTCCTTCAATGGGCTCTAAG+GGG	+	chr1_3:64034753-64034772	None:intergenic	45.0%
!	CATTCCTTCAATGGGCTCTA+AGG	+	chr1_3:64034755-64034774	None:intergenic	45.0%
	CTTAGAGCCCATTGAAGGAA+TGG	-	chr1_3:64034753-64034772	Msa0132760:CDS	45.0%
	ACAAGGTACGCTCAGAACAA+AGG	-	chr1_3:64034811-64034830	Msa0132760:CDS	45.0%
	TACACATGATGAAACCAGGG+TGG	-	chr1_3:64034963-64034982	Msa0132760:CDS	45.0%
	ACACATGATGAAACCAGGGT+GGG	-	chr1_3:64034964-64034983	Msa0132760:CDS	45.0%
!	CACTTCTAGGAGAAAACAGC+TGG	+	chr1_3:64035065-64035084	None:intergenic	45.0%
	CGAATAGACTGTGCACTTCT+AGG	+	chr1_3:64035078-64035097	None:intergenic	45.0%
	GTCTTATGCACTGAAAGTGG+CGG	-	chr1_3:64035177-64035196	Msa0132760:CDS	45.0%
!	CATTGGTGATAGCAGTGTGT+TGG	+	chr1_3:64035632-64035651	None:intergenic	45.0%
	CTATCACCAATGCAGTTGCA+AGG	-	chr1_3:64035640-64035659	Msa0132760:CDS	45.0%
	AAACACCCTTGCAACTGCAT+TGG	+	chr1_3:64035649-64035668	None:intergenic	45.0%
!!	AGTTGCAAGGGTGTTTCCAA+AGG	-	chr1_3:64035653-64035672	Msa0132760:CDS	45.0%
!	ACGGGTTGCAAGATAATCAG+TGG	-	chr1_3:64035841-64035860	Msa0132760:CDS	45.0%
	CTGGAATGTCTACCACTCAT+AGG	-	chr1_3:64035928-64035947	Msa0132760:CDS	45.0%
	TCTTGATGCACGTTACAACG+AGG	-	chr1_3:64036034-64036053	Msa0132760:CDS	45.0%
	ATGCACGTTACAACGAGGAA+AGG	-	chr1_3:64036039-64036058	Msa0132760:CDS	45.0%
	TGCACGTTACAACGAGGAAA+GGG	-	chr1_3:64036040-64036059	Msa0132760:CDS	45.0%
!!	TCCTTCAATGGGCTCTAAGG+GGG	+	chr1_3:64034752-64034771	None:intergenic	50.0%
	ACTTAGTAACACGGCCCATG+CGG	+	chr1_3:64034846-64034865	None:intergenic	50.0%
	GCGTTTCAAATGGATGCCGA+AGG	-	chr1_3:64035342-64035361	Msa0132760:CDS	50.0%
!!	TCCTTCAATGGGCTCTAAGG+GGG	+	chr1_3:64034752-64034771	None:intergenic	50.0%
	ACTTAGTAACACGGCCCATG+CGG	+	chr1_3:64034846-64034865	None:intergenic	50.0%
	GCGTTTCAAATGGATGCCGA+AGG	-	chr1_3:64035342-64035361	Msa0132760:CDS	50.0%
!!	CCTTCAATGGGCTCTAAGGG+GGG	+	chr1_3:64034751-64034770	None:intergenic	55.0%
!	TTTGGCTCGAAACCCCTCCT+TGG	+	chr1_3:64034911-64034930	None:intergenic	55.0%
	TGAAACCAGGGTGGGATGCA+AGG	-	chr1_3:64034972-64034991	Msa0132760:CDS	55.0%
!!	CCTTCAATGGGCTCTAAGGG+GGG	+	chr1_3:64034751-64034770	None:intergenic	55.0%
!	TTTGGCTCGAAACCCCTCCT+TGG	+	chr1_3:64034911-64034930	None:intergenic	55.0%
	TGAAACCAGGGTGGGATGCA+AGG	-	chr1_3:64034972-64034991	Msa0132760:CDS	55.0%
!	TCTAAGGGGGGCTCTGATGG+TGG	+	chr1_3:64034739-64034758	None:intergenic	60.0%
	CCCCCCTTAGAGCCCATTGA+AGG	-	chr1_3:64034748-64034767	Msa0132760:CDS	60.0%
!	TCTAAGGGGGGCTCTGATGG+TGG	+	chr1_3:64034739-64034758	None:intergenic	60.0%
	CCCCCCTTAGAGCCCATTGA+AGG	-	chr1_3:64034748-64034767	Msa0132760:CDS	60.0%
!!	GGCTCTAAGGGGGGCTCTGA+TGG	+	chr1_3:64034742-64034761	None:intergenic	65.0%
	CCAGGGTGGGATGCAAGGCA+CGG	-	chr1_3:64034977-64034996	Msa0132760:CDS	65.0%
!!	GGCTCTAAGGGGGGCTCTGA+TGG	+	chr1_3:64034742-64034761	None:intergenic	65.0%
	CCAGGGTGGGATGCAAGGCA+CGG	-	chr1_3:64034977-64034996	Msa0132760:CDS	65.0%
	CCGTGCCTTGCATCCCACCC+TGG	+	chr1_3:64034980-64034999	None:intergenic	70.0%
	CCGTGCCTTGCATCCCACCC+TGG	+	chr1_3:64034980-64034999	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	64034634	64036667	64034634	ID=Msa0132760;Name=Msa0132760
chr1_3	mRNA	64034634	64036667	64034634	ID=Msa0132760-mRNA-1;Parent=Msa0132760;Name=Msa0132760-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|677
chr1_3	exon	64034634	64036667	64034634	ID=Msa0132760-mRNA-1:exon:21448;Parent=Msa0132760-mRNA-1
chr1_3	CDS	64034634	64036667	64034634	ID=Msa0132760-mRNA-1:cds;Parent=Msa0132760-mRNA-1

Gene Sequence

>Msa0132760

ATGGCAGGTCATGAGATGACAGATATGGATGGAGATTTTACCATGGTTGTCTCAAATGATACACATGTCAGTGCTCTTGATGTGAAGCCTCTCAATAGTGAACCACCATCAGAGCCCCCCTTAGAGCCCATTGAAGGAATGGAGTTTAAATCATTTGAAGAAGCAAAGAGTTACTACACAAGGTACGCTCAGAACAAAGGTTTTAGTTTCCGCATGGGCCGTGTTACTAAGTCAAGAACAAACGGTATGATAATTGGTCAAGAAATTCTTTGTTCCAAGGAGGGGTTTCGAGCCAAAAAGTATGTGAAACAAGGAAATAGTAGTCTTATTACACATGATGAAACCAGGGTGGGATGCAAGGCACGGTTATATTTAAAGAAAAATAATGATATATGGATTGTTTCTAGATTTGTAAGTGACCATAATCACCAGCTGTTTTCTCCTAGAAGTGCACAGTCTATTCGCGTACATAGAAAGAAAACTAAAGTGCAAAAGACACTTACTGATGTGCTTGATGAATCTGGTTTAGGCAAAACAACATCTGTCTTATGCACTGAAAGTGGCGGTATTGATAATTTTGGATCTAGCCAACAAGATGTTATCAATTACTTAAGTGTACAGAGACAAAAACAATTTGAAAACAGAGATGCTCAATTGATGTTATCATACTTTAAGAATTGTCAGTTGAAGAATACTGGGTTTTTCTATGCGTTTCAAATGGATGCCGAAGGAAAATTAACTAATTGCTTTTGGGTTGATTCAAGGTCTAGAGTTGCTTACAAATATTTTGGTGATGTGGTTACTTTTGATCCAACATACTTGAAAAATAAATACAAAACTCCTTTTGTTCCAATTACTGGAGTTAATCATCATCAACAATCAATTCTTTTTGGTTGTGCTCTCTTGTGGGATGAAACTGTAGAGAGTTTTGTTTGGTTGCTAAGTACTTGGTTAGAAGCAATGAATGGGGTTTGCCCTAAAACTATTATCACAGACCAACACACTGCTATCACCAATGCAGTTGCAAGGGTGTTTCCAAAGGTTAATCACCATTATTGCATGTGGCATATTGAGGAAAAAGTTCCAGAGCACATGAATCAGATCTATCATGGACACAGTGAATTTAAAAATCATTTTTACAAGTGCATTCACCAATCTATCACAATTGAAGAATTTGAGTCTGAATGGGAAGCAATGATTGATAAATACGGGTTGCAAGATAATCAGTGGCTAGTGAAGATATACTCAATTCGATCAAAATGGATTCCTGCATATGTACATCATAATTTTTGTGCTGGAATGTCTACCACTCATAGGAGTGAAAGTATGAATAAGTTTTTTAAGGATTTCTTGAATTCAAGTACTCCATTGAGCAAGTTTCTGACACAATATGAGAAAGCTCTTGATGCACGTTACAACGAGGAAAGGGAGAAAACTGTCAAGACGATGAATTCAAAACCACTTTTGCGAACTTTATACCCCATGGAAGAAGAAGCTTCAAAAATTTATACAGGAAAATTGTTTGAGATATTTCAAGATGAGTTGGTTGGCTCTCAAATGTTTACCACAGAAAAAGTTGAATTTTCTGATGAAGTGGCAACATACAAAGTTCATGAAATTTACAAAGAGAAGCCTAAATATCATGTGACTTTTCATGTAACTTCAAAAGAAGCAACTTGTACTTGTCAAAAGTTTGAATCGTTTGGTATTCTTTGTAGGCATATCTTAACTGTGTTCCTAAAGAAAAAGGTACGTTATCTCCCTTCACAGTATGTTCTACAACGATGGACCCGAAATGCTAAGAAAGAAAAATTTGAAGGATTGACAATTGAAGAATTTGAAGGAGGAAACAAAGCTTCTAGCACTTCATTGTTTAATAGTGTTATGGTTCGTTCACTGGAATTTTCTGAAAGAGCTTCATGGTCAAAAAAACATCATGATATTGCAATTCAATGTTTGCAAAATGCAATTTCAAAACTTGATCTATTAGAGCTTGAAGAATCAAATGAGGAGTTTGTTAATTTGACATCTTAA

Protein sequence

>Msa0132760

MAGHEMTDMDGDFTMVVSNDTHVSALDVKPLNSEPPSEPPLEPIEGMEFKSFEEAKSYYTRYAQNKGFSFRMGRVTKSRTNGMIIGQEILCSKEGFRAKKYVKQGNSSLITHDETRVGCKARLYLKKNNDIWIVSRFVSDHNHQLFSPRSAQSIRVHRKKTKVQKTLTDVLDESGLGKTTSVLCTESGGIDNFGSSQQDVINYLSVQRQKQFENRDAQLMLSYFKNCQLKNTGFFYAFQMDAEGKLTNCFWVDSRSRVAYKYFGDVVTFDPTYLKNKYKTPFVPITGVNHHQQSILFGCALLWDETVESFVWLLSTWLEAMNGVCPKTIITDQHTAITNAVARVFPKVNHHYCMWHIEEKVPEHMNQIYHGHSEFKNHFYKCIHQSITIEEFESEWEAMIDKYGLQDNQWLVKIYSIRSKWIPAYVHHNFCAGMSTTHRSESMNKFFKDFLNSSTPLSKFLTQYEKALDARYNEEREKTVKTMNSKPLLRTLYPMEEEASKIYTGKLFEIFQDELVGSQMFTTEKVEFSDEVATYKVHEIYKEKPKYHVTFHVTSKEATCTCQKFESFGILCRHILTVFLKKKVRYLPSQYVLQRWTRNAKKEKFEGLTIEEFEGGNKASSTSLFNSVMVRSLEFSERASWSKKHHDIAIQCLQNAISKLDLLELEESNEEFVNLTS*