Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466610 | XP_013459503.1 | 23.355 | 304 | 198 | 4 | 2 | 271 | 301 | 603 | 5.48e-17 | 93.6 |
| Msa1466610 | XP_013459503.1 | 26.027 | 219 | 154 | 4 | 2 | 216 | 198 | 412 | 5.30e-15 | 87.4 |
| Msa1466610 | XP_013459503.1 | 28.155 | 309 | 171 | 11 | 2 | 267 | 402 | 702 | 5.11e-25 | 117 |
| Msa1466610 | XP_013459503.1 | 97.697 | 304 | 7 | 0 | 1 | 304 | 501 | 804 | 0.0 | 626 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466610 | sp|Q9SY02|PP301_ARATH | 46.465 | 297 | 157 | 2 | 2 | 297 | 372 | 667 | 4.43e-92 | 292 |
| Msa1466610 | sp|Q9SY02|PP301_ARATH | 28.571 | 168 | 107 | 3 | 1 | 168 | 247 | 401 | 3.35e-15 | 79.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466610 | A0A072UUM5 | 23.355 | 304 | 198 | 4 | 2 | 271 | 301 | 603 | 2.62e-17 | 93.6 |
| Msa1466610 | A0A072UUM5 | 26.027 | 219 | 154 | 4 | 2 | 216 | 198 | 412 | 2.53e-15 | 87.4 |
| Msa1466610 | A0A072UUM5 | 28.155 | 309 | 171 | 11 | 2 | 267 | 402 | 702 | 2.44e-25 | 117 |
| Msa1466610 | A0A072UUM5 | 97.697 | 304 | 7 | 0 | 1 | 304 | 501 | 804 | 0.0 | 626 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0084380 | Msa1466610 | 0.805348 | 1.394142e-49 | -8.615850e-47 |
| Msa0160550 | Msa1466610 | 0.802014 | 6.859489e-49 | -8.615850e-47 |
| Msa0333030 | Msa1466610 | 0.803176 | 3.950284e-49 | -8.615850e-47 |
| Msa0610430 | Msa1466610 | 0.808194 | 3.492026e-50 | -8.615850e-47 |
| Msa0743950 | Msa1466610 | 0.804323 | 2.283146e-49 | -8.615850e-47 |
| Msa0764130 | Msa1466610 | -0.801021 | 1.095806e-48 | -8.615850e-47 |
| Msa0947660 | Msa1466610 | 0.802570 | 5.269949e-49 | -8.615850e-47 |
| Msa0974070 | Msa1466610 | 0.808145 | 3.576980e-50 | -8.615850e-47 |
| Msa1014540 | Msa1466610 | 0.806663 | 7.372749e-50 | -8.615850e-47 |
| Msa1186950 | Msa1466610 | -0.803878 | 2.825358e-49 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466610 | MtrunA17_Chr3g0093831 | 97.697 | 304 | 7 | 0 | 1 | 304 | 501 | 804 | 0.0 | 626 |
| Msa1466610 | MtrunA17_Chr3g0093831 | 28.155 | 309 | 171 | 11 | 2 | 267 | 402 | 702 | 4.70e-29 | 117 |
| Msa1466610 | MtrunA17_Chr3g0093831 | 23.355 | 304 | 198 | 4 | 2 | 271 | 301 | 603 | 5.04e-21 | 93.6 |
| Msa1466610 | MtrunA17_Chr3g0093831 | 26.027 | 219 | 154 | 4 | 2 | 216 | 198 | 412 | 4.87e-19 | 87.4 |
| Msa1466610 | MtrunA17_Chr3g0093831 | 30.263 | 152 | 104 | 2 | 2 | 152 | 98 | 248 | 1.01e-13 | 71.6 |
| Msa1466610 | MtrunA17_Chr8g0392791 | 46.441 | 295 | 156 | 2 | 2 | 295 | 357 | 650 | 2.47e-92 | 290 |
| Msa1466610 | MtrunA17_Chr8g0392791 | 23.757 | 181 | 125 | 3 | 1 | 181 | 232 | 399 | 2.43e-14 | 73.6 |
| Msa1466610 | MtrunA17_Chr8g0392791 | 21.910 | 356 | 201 | 12 | 2 | 297 | 78 | 416 | 7.61e-13 | 68.9 |
| Msa1466610 | MtrunA17_Chr8g0392791 | 23.860 | 285 | 173 | 9 | 15 | 271 | 27 | 295 | 1.47e-11 | 65.1 |
| Msa1466610 | MtrunA17_Chr8g0364661 | 43.051 | 295 | 166 | 2 | 2 | 295 | 281 | 574 | 4.52e-92 | 287 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1466610 | AT4G02750.1 | 46.465 | 297 | 157 | 2 | 2 | 297 | 372 | 667 | 4.50e-93 | 292 |
| Msa1466610 | AT4G02750.1 | 28.571 | 168 | 107 | 3 | 1 | 168 | 247 | 401 | 3.41e-16 | 79.3 |
| Msa1466610 | AT4G02750.1 | 23.279 | 305 | 198 | 9 | 2 | 293 | 155 | 436 | 2.88e-11 | 64.7 |
| Msa1466610 | AT3G49142.1 | 44.595 | 296 | 162 | 2 | 2 | 296 | 272 | 566 | 1.66e-91 | 286 |
| Msa1466610 | AT3G49142.1 | 27.857 | 280 | 183 | 7 | 2 | 271 | 103 | 373 | 2.65e-21 | 94.7 |
| Msa1466610 | AT4G18750.1 | 44.000 | 300 | 159 | 4 | 1 | 295 | 460 | 755 | 8.66e-86 | 275 |
| Msa1466610 | AT4G18750.1 | 27.442 | 215 | 154 | 2 | 2 | 216 | 259 | 471 | 3.63e-25 | 105 |
| Msa1466610 | AT4G18750.1 | 31.646 | 158 | 108 | 0 | 5 | 162 | 363 | 520 | 1.31e-23 | 101 |
| Msa1466610 | AT4G18750.1 | 26.198 | 313 | 181 | 6 | 1 | 271 | 157 | 461 | 2.36e-22 | 97.8 |
| Msa1466610 | AT3G46790.1 | 39.934 | 303 | 178 | 3 | 1 | 300 | 245 | 546 | 1.18e-83 | 265 |
Find 0 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTTACAGAAATGAGACTTTC+CGG | + | tig0047947:81-100 | Msa1466610:CDS | 30.0% | |
| CTTGGTATACAAATCCAAAA+GGG | - | tig0047947:241-260 | Msa1466610:intergenic | 30.0% | |
| ACTTGGTATACAAATCCAAA+AGG | - | tig0047947:242-261 | Msa1466610:intergenic | 30.0% | |
| CTACATCTTTGTGTTGAATA+CGG | - | tig0047947:299-318 | Msa1466610:intergenic | 30.0% | |
| CAAAAGATAAGCCTCTAATA+AGG | - | tig0047947:596-615 | Msa1466610:intergenic | 30.0% | |
| TTGAACCTGATGCTAATATA+TGG | + | tig0047947:613-632 | Msa1466610:CDS | 30.0% | |
| TGAACCTGATGCTAATATAT+GGG | + | tig0047947:614-633 | Msa1466610:CDS | 30.0% | |
| ATATATGGGAATGTGGAATT+GGG | + | tig0047947:660-679 | Msa1466610:CDS | 30.0% | |
| TTAGGGAATTGATGAAATCA+AGG | + | tig0047947:793-812 | Msa1466610:CDS | 30.0% | |
| TAGGGAATTGATGAAATCAA+GGG | + | tig0047947:794-813 | Msa1466610:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0047947 | gene | 3 | 917 | 3 | ID=Msa1466610;Name=Msa1466610 |
| tig0047947 | mRNA | 3 | 917 | 3 | ID=Msa1466610-mRNA-1;Parent=Msa1466610;Name=Msa1466610-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|304 |
| tig0047947 | exon | 3 | 917 | 3 | ID=Msa1466610-mRNA-1:exon:25107;Parent=Msa1466610-mRNA-1 |
| tig0047947 | CDS | 3 | 917 | 3 | ID=Msa1466610-mRNA-1:cds;Parent=Msa1466610-mRNA-1 |
| Gene Sequence |
>Msa1466610 GTCAAGGATAAAGTTTCCTACAATATACTAATTATAGGTTATTCTCAAACAACTAACAGCTCAGAGTCTCTAAATTTGTTTACAGAAATGAGACTTTCCGGTATGACGCCTGACATTGTTTCGTTCATCGGTATCATATCCGCTTGTGCACATCTGTCTTCCATAAAACAAGGTAAGGAGATTCATGGACATCTGGTAAGAAAGCTTTTCCATACTCATCTTTTTGCAGCAAATTCCCTTTTGGATTTGTATACCAAGTGTGGTCGAATAGATCTTGCAACTAAAGTCTTCGACCGTATTCAACACAAAGATGTAGCCTCATGGAATACTATGATTTTAGGGTATGGTATGCGTGGTGAATTCGAAACTGCAATCAACCTCTTTGAAGCAATGAAGGAAGATGATGGTGTGGAATATGATTCAGTTTCATATATTGCAGTTTTATCGGCATGTAGTCATGGAGGACTGATCGAGAAAGGGAATAAATATTTTAAGCAAATGCAAGATTATAATATTGAGCCAACACATACGCACTATGCTTGTATGGTCGATCTTCTTGGAAGAGCAGGCCAAATTGATGAAGCTGCAAACCTTATTAGAGGCTTATCTTTTGAACCTGATGCTAATATATGGGGTGCACTACTTGGAGCTTGCAGGATATATGGGAATGTGGAATTGGGTCATTGGGCTGCTGAGCATTTGTTTAAATTGAAACCTGACCATTGTGGATACTATATACTGCTTTCAAATATGTATGCTGAGGCTGGAAGATGGGATGAGGCAAATAGGGTTAGGGAATTGATGAAATCAAGGGGAGCTAAGAAAAATCCTGGTTGTAGTTGGGTTCAAATTGGAGACCAAGTGCATGCTTTTCTAGTTGGTGAGAAAATAAAGAGCTTAGATACTGGATTTTGA |
| Protein sequence |
>Msa1466610 VKDKVSYNILIIGYSQTTNSSESLNLFTEMRLSGMTPDIVSFIGIISACAHLSSIKQGKEIHGHLVRKLFHTHLFAANSLLDLYTKCGRIDLATKVFDRIQHKDVASWNTMILGYGMRGEFETAINLFEAMKEDDGVEYDSVSYIAVLSACSHGGLIEKGNKYFKQMQDYNIEPTHTHYACMVDLLGRAGQIDEAANLIRGLSFEPDANIWGALLGACRIYGNVELGHWAAEHLFKLKPDHCGYYILLSNMYAEAGRWDEANRVRELMKSRGAKKNPGCSWVQIGDQVHAFLVGEKIKSLDTGF* |
