AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1379500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 18 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAGTTTGCCTAGAGCTAT+TGG 0.284593 tig0020279:-136062 None:intergenic
GGTCGAAAACATGGTTGAAA+TGG 0.329615 tig0020279:-134787 None:intergenic
GGAAGAGCAATAAGAAATAA+AGG 0.346296 tig0020279:+135800 Msa1379500:CDS
GAAATCTCCAAAATGACAAT+AGG 0.421681 tig0020279:+136019 Msa1379500:three_prime_UTR
GAAAGCAGATGAATCAAAGA+AGG 0.451708 tig0020279:+135748 Msa1379500:CDS
AAAATGACAATAGGTTGAAA+AGG 0.473813 tig0020279:+136028 Msa1379500:three_prime_UTR
GTCGAAAACATGGTTGAAAT+GGG 0.474047 tig0020279:-134786 None:intergenic
TTCTTCTTCTCCTTCGTCTG+AGG 0.523655 tig0020279:-134744 None:intergenic
GGAAATTACGGTCGAAAACA+TGG 0.524494 tig0020279:-134796 None:intergenic
TTAATTTATGATCGAAGAAG+TGG 0.529345 tig0020279:+135825 Msa1379500:exon
AAAATGCAAAGAAAGGCAAA+GGG 0.535875 tig0020279:+135779 Msa1379500:CDS
CATGTGTGTCCAATAGCTCT+AGG 0.550624 tig0020279:+136053 Msa1379500:three_prime_UTR
ATTTATGATCGAAGAAGTGG+AGG 0.572124 tig0020279:+135828 Msa1379500:exon
ATGGTATGCACCTCAGACGA+AGG 0.594976 tig0020279:+134734 Msa1379500:CDS
AAAGCAGATGAATCAAAGAA+GGG 0.629387 tig0020279:+135749 Msa1379500:CDS
GCAAACTTAGAAATTACAAG+AGG 0.646201 tig0020279:+136075 Msa1379500:three_prime_UTR
TTTATGATCGAAGAAGTGGA+GGG 0.648481 tig0020279:+135829 Msa1379500:exon
GCAGATGAATCAAAGAAGGG+AGG 0.649662 tig0020279:+135752 Msa1379500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTTGTAATATTTTTAT+TGG - tig0020279:134972-134991 None:intergenic 10.0%
!!! ATTATTATGAATTTTATTTC+CGG + tig0020279:135869-135888 Msa1379500:three_prime_UTR 10.0%
!!! TTCTTTTGTAATATTTTTAT+TGG - tig0020279:134972-134991 None:intergenic 10.0%
!!! ATTATTATGAATTTTATTTC+CGG + tig0020279:135869-135888 Msa1379500:three_prime_UTR 10.0%
!! ATATTTAATCAAGGAAATTA+CGG - tig0020279:134811-134830 None:intergenic 15.0%
!! TAATTTCCTTGATTAAATAT+AGG + tig0020279:134811-134830 Msa1379500:intron 15.0%
!! ATATTTAATCAAGGAAATTA+CGG - tig0020279:134811-134830 None:intergenic 15.0%
!! TAATTTCCTTGATTAAATAT+AGG + tig0020279:134811-134830 Msa1379500:intron 15.0%
!! TTGTTACCTATATTTAATCA+AGG - tig0020279:134820-134839 None:intergenic 20.0%
!! CAAATAGGTAAAAGAAAAAA+GGG - tig0020279:135027-135046 None:intergenic 20.0%
!! ACAAATAGGTAAAAGAAAAA+AGG - tig0020279:135028-135047 None:intergenic 20.0%
!! TGATTATTTACCTAAACTAA+GGG - tig0020279:135104-135123 None:intergenic 20.0%
!! ATGATTATTTACCTAAACTA+AGG - tig0020279:135105-135124 None:intergenic 20.0%
!! TGTTACTCTAGATATAAATT+AGG + tig0020279:135236-135255 Msa1379500:intron 20.0%
!! GTTACTCTAGATATAAATTA+GGG + tig0020279:135237-135256 Msa1379500:intron 20.0%
!! TCAAAAACTTAACTATCAAA+CGG - tig0020279:135410-135429 None:intergenic 20.0%
!!! ATAACTTTTATTCACCATTT+AGG - tig0020279:135593-135612 None:intergenic 20.0%
!! TTGTTACCTATATTTAATCA+AGG - tig0020279:134820-134839 None:intergenic 20.0%
!! CAAATAGGTAAAAGAAAAAA+GGG - tig0020279:135027-135046 None:intergenic 20.0%
!! ACAAATAGGTAAAAGAAAAA+AGG - tig0020279:135028-135047 None:intergenic 20.0%
!! TGATTATTTACCTAAACTAA+GGG - tig0020279:135104-135123 None:intergenic 20.0%
!! ATGATTATTTACCTAAACTA+AGG - tig0020279:135105-135124 None:intergenic 20.0%
!! TGTTACTCTAGATATAAATT+AGG + tig0020279:135236-135255 Msa1379500:intron 20.0%
!! GTTACTCTAGATATAAATTA+GGG + tig0020279:135237-135256 Msa1379500:intron 20.0%
!! TCAAAAACTTAACTATCAAA+CGG - tig0020279:135410-135429 None:intergenic 20.0%
!!! ATAACTTTTATTCACCATTT+AGG - tig0020279:135593-135612 None:intergenic 20.0%
! AAAAAACAAATCAAGAAGCT+TGG - tig0020279:134858-134877 None:intergenic 25.0%
!!! GCTTCTTGATTTGTTTTTTT+TGG + tig0020279:134859-134878 Msa1379500:intron 25.0%
! GAAAATAGTTAGAAACATGA+AGG + tig0020279:135539-135558 Msa1379500:intron 25.0%
! TAAATCAACCAACATGTATT+AGG - tig0020279:135691-135710 None:intergenic 25.0%
! AGGAAAAAAAATGCAAAGAA+AGG + tig0020279:135772-135791 Msa1379500:CDS 25.0%
! TTAATTTATGATCGAAGAAG+TGG + tig0020279:135825-135844 Msa1379500:exon 25.0%
! GTAAAAAGTAAATAATGCAC+AGG + tig0020279:135940-135959 Msa1379500:three_prime_UTR 25.0%
!!! TTTTCAACCTATTGTCATTT+TGG - tig0020279:136029-136048 None:intergenic 25.0%
!! AAAATGACAATAGGTTGAAA+AGG + tig0020279:136028-136047 Msa1379500:three_prime_UTR 25.0%
! AAAAAACAAATCAAGAAGCT+TGG - tig0020279:134858-134877 None:intergenic 25.0%
!!! GCTTCTTGATTTGTTTTTTT+TGG + tig0020279:134859-134878 Msa1379500:intron 25.0%
! GAAAATAGTTAGAAACATGA+AGG + tig0020279:135539-135558 Msa1379500:intron 25.0%
! TAAATCAACCAACATGTATT+AGG - tig0020279:135691-135710 None:intergenic 25.0%
! AGGAAAAAAAATGCAAAGAA+AGG + tig0020279:135772-135791 Msa1379500:CDS 25.0%
! TTAATTTATGATCGAAGAAG+TGG + tig0020279:135825-135844 Msa1379500:exon 25.0%
! GTAAAAAGTAAATAATGCAC+AGG + tig0020279:135940-135959 Msa1379500:three_prime_UTR 25.0%
!!! TTTTCAACCTATTGTCATTT+TGG - tig0020279:136029-136048 None:intergenic 25.0%
!! AAAATGACAATAGGTTGAAA+AGG + tig0020279:136028-136047 Msa1379500:three_prime_UTR 25.0%
TAGGTAAAAGAAAAAAGGGA+AGG - tig0020279:135023-135042 None:intergenic 30.0%
TTACCTATTTGTAATCACAG+TGG + tig0020279:135036-135055 Msa1379500:intron 30.0%
TTACCACTGTGATTACAAAT+AGG - tig0020279:135042-135061 None:intergenic 30.0%
! TAACTTTTACAAAATCACCC+TGG + tig0020279:135074-135093 Msa1379500:intron 30.0%
TGGATGTAAAGTTGAGAATA+CGG + tig0020279:135473-135492 Msa1379500:intron 30.0%
TTGAGAATACGGTTAAATTC+TGG + tig0020279:135484-135503 Msa1379500:intron 30.0%
! TTAGAAACATGAAGGCTTTT+GGG + tig0020279:135547-135566 Msa1379500:intron 30.0%
TGTTTCAACCTAATACATGT+TGG + tig0020279:135680-135699 Msa1379500:intron 30.0%
! ATACATGTTGGTTGATTTAC+AGG + tig0020279:135692-135711 Msa1379500:intron 30.0%
! TACATGTTGGTTGATTTACA+GGG + tig0020279:135693-135712 Msa1379500:intron 30.0%
! AACACGTATTGTATTTTGCA+AGG + tig0020279:135718-135737 Msa1379500:CDS 30.0%
! ACACGTATTGTATTTTGCAA+GGG + tig0020279:135719-135738 Msa1379500:CDS 30.0%
AAAGCAGATGAATCAAAGAA+GGG + tig0020279:135749-135768 Msa1379500:CDS 30.0%
AAAAATGCAAAGAAAGGCAA+AGG + tig0020279:135778-135797 Msa1379500:CDS 30.0%
AAAATGCAAAGAAAGGCAAA+GGG + tig0020279:135779-135798 Msa1379500:CDS 30.0%
GGAAGAGCAATAAGAAATAA+AGG + tig0020279:135800-135819 Msa1379500:CDS 30.0%
!!! GTGTAGACCTCTTTTTTATA+TGG - tig0020279:135898-135917 None:intergenic 30.0%
GAAATCTCCAAAATGACAAT+AGG + tig0020279:136019-136038 Msa1379500:three_prime_UTR 30.0%
GCAAACTTAGAAATTACAAG+AGG + tig0020279:136075-136094 Msa1379500:three_prime_UTR 30.0%
TAGGTAAAAGAAAAAAGGGA+AGG - tig0020279:135023-135042 None:intergenic 30.0%
TTACCTATTTGTAATCACAG+TGG + tig0020279:135036-135055 Msa1379500:intron 30.0%
TTACCACTGTGATTACAAAT+AGG - tig0020279:135042-135061 None:intergenic 30.0%
! TAACTTTTACAAAATCACCC+TGG + tig0020279:135074-135093 Msa1379500:intron 30.0%
TGGATGTAAAGTTGAGAATA+CGG + tig0020279:135473-135492 Msa1379500:intron 30.0%
TTGAGAATACGGTTAAATTC+TGG + tig0020279:135484-135503 Msa1379500:intron 30.0%
! TTAGAAACATGAAGGCTTTT+GGG + tig0020279:135547-135566 Msa1379500:intron 30.0%
TGTTTCAACCTAATACATGT+TGG + tig0020279:135680-135699 Msa1379500:intron 30.0%
! ATACATGTTGGTTGATTTAC+AGG + tig0020279:135692-135711 Msa1379500:intron 30.0%
! TACATGTTGGTTGATTTACA+GGG + tig0020279:135693-135712 Msa1379500:intron 30.0%
! AACACGTATTGTATTTTGCA+AGG + tig0020279:135718-135737 Msa1379500:CDS 30.0%
! ACACGTATTGTATTTTGCAA+GGG + tig0020279:135719-135738 Msa1379500:CDS 30.0%
AAAGCAGATGAATCAAAGAA+GGG + tig0020279:135749-135768 Msa1379500:CDS 30.0%
AAAAATGCAAAGAAAGGCAA+AGG + tig0020279:135778-135797 Msa1379500:CDS 30.0%
AAAATGCAAAGAAAGGCAAA+GGG + tig0020279:135779-135798 Msa1379500:CDS 30.0%
GGAAGAGCAATAAGAAATAA+AGG + tig0020279:135800-135819 Msa1379500:CDS 30.0%
!!! GTGTAGACCTCTTTTTTATA+TGG - tig0020279:135898-135917 None:intergenic 30.0%
GAAATCTCCAAAATGACAAT+AGG + tig0020279:136019-136038 Msa1379500:three_prime_UTR 30.0%
GCAAACTTAGAAATTACAAG+AGG + tig0020279:136075-136094 Msa1379500:three_prime_UTR 30.0%
GAGAAGAAGAAGAAGAAGAT+GGG + tig0020279:134756-134775 Msa1379500:CDS 35.0%
GTCGAAAACATGGTTGAAAT+GGG - tig0020279:134789-134808 None:intergenic 35.0%
GCGTTGTGGAGAAATAAAAT+TGG + tig0020279:135163-135182 Msa1379500:intron 35.0%
GAATTTATAGCCAACTGAAG+CGG + tig0020279:135440-135459 Msa1379500:intron 35.0%
CTCAACTTTACATCCAATGA+TGG - tig0020279:135469-135488 None:intergenic 35.0%
! TGGAAAATCCATCCAAGTTT+TGG + tig0020279:135504-135523 Msa1379500:intron 35.0%
AAATATCCGTCACCAAAACT+TGG - tig0020279:135519-135538 None:intergenic 35.0%
! GTTAGAAACATGAAGGCTTT+TGG + tig0020279:135546-135565 Msa1379500:intron 35.0%
TTATTCACCATTTAGGCAGT+AGG - tig0020279:135586-135605 None:intergenic 35.0%
GAAAGCAGATGAATCAAAGA+AGG + tig0020279:135748-135767 Msa1379500:CDS 35.0%
ATTTATGATCGAAGAAGTGG+AGG + tig0020279:135828-135847 Msa1379500:exon 35.0%
TTTATGATCGAAGAAGTGGA+GGG + tig0020279:135829-135848 Msa1379500:exon 35.0%
!!! CCTCTTTTTTATATGGACAC+CGG - tig0020279:135891-135910 None:intergenic 35.0%
GAGAAGAAGAAGAAGAAGAT+GGG + tig0020279:134756-134775 Msa1379500:CDS 35.0%
GTCGAAAACATGGTTGAAAT+GGG - tig0020279:134789-134808 None:intergenic 35.0%
GCGTTGTGGAGAAATAAAAT+TGG + tig0020279:135163-135182 Msa1379500:intron 35.0%
GAATTTATAGCCAACTGAAG+CGG + tig0020279:135440-135459 Msa1379500:intron 35.0%
CTCAACTTTACATCCAATGA+TGG - tig0020279:135469-135488 None:intergenic 35.0%
! TGGAAAATCCATCCAAGTTT+TGG + tig0020279:135504-135523 Msa1379500:intron 35.0%
AAATATCCGTCACCAAAACT+TGG - tig0020279:135519-135538 None:intergenic 35.0%
! GTTAGAAACATGAAGGCTTT+TGG + tig0020279:135546-135565 Msa1379500:intron 35.0%
TTATTCACCATTTAGGCAGT+AGG - tig0020279:135586-135605 None:intergenic 35.0%
GAAAGCAGATGAATCAAAGA+AGG + tig0020279:135748-135767 Msa1379500:CDS 35.0%
ATTTATGATCGAAGAAGTGG+AGG + tig0020279:135828-135847 Msa1379500:exon 35.0%
TTTATGATCGAAGAAGTGGA+GGG + tig0020279:135829-135848 Msa1379500:exon 35.0%
!!! CCTCTTTTTTATATGGACAC+CGG - tig0020279:135891-135910 None:intergenic 35.0%
GGAGAAGAAGAAGAAGAAGA+TGG + tig0020279:134755-134774 Msa1379500:CDS 40.0%
GGTCGAAAACATGGTTGAAA+TGG - tig0020279:134790-134809 None:intergenic 40.0%
GGAAATTACGGTCGAAAACA+TGG - tig0020279:134799-134818 None:intergenic 40.0%
! AGGGCTCTATGAATCACTTT+TGG + tig0020279:135256-135275 Msa1379500:intron 40.0%
!! ATCCATCCAAGTTTTGGTGA+CGG + tig0020279:135510-135529 Msa1379500:intron 40.0%
!!! AACATGAAGGCTTTTGGGAT+TGG + tig0020279:135552-135571 Msa1379500:intron 40.0%
!!! ACATGAAGGCTTTTGGGATT+GGG + tig0020279:135553-135572 Msa1379500:intron 40.0%
CCGGTGTCCATATAAAAAAG+AGG + tig0020279:135888-135907 Msa1379500:three_prime_UTR 40.0%
CTAAGTTTGCCTAGAGCTAT+TGG - tig0020279:136065-136084 None:intergenic 40.0%
GGAGAAGAAGAAGAAGAAGA+TGG + tig0020279:134755-134774 Msa1379500:CDS 40.0%
GGTCGAAAACATGGTTGAAA+TGG - tig0020279:134790-134809 None:intergenic 40.0%
GGAAATTACGGTCGAAAACA+TGG - tig0020279:134799-134818 None:intergenic 40.0%
! AGGGCTCTATGAATCACTTT+TGG + tig0020279:135256-135275 Msa1379500:intron 40.0%
!! ATCCATCCAAGTTTTGGTGA+CGG + tig0020279:135510-135529 Msa1379500:intron 40.0%
!!! AACATGAAGGCTTTTGGGAT+TGG + tig0020279:135552-135571 Msa1379500:intron 40.0%
!!! ACATGAAGGCTTTTGGGATT+GGG + tig0020279:135553-135572 Msa1379500:intron 40.0%
CCGGTGTCCATATAAAAAAG+AGG + tig0020279:135888-135907 Msa1379500:three_prime_UTR 40.0%
CTAAGTTTGCCTAGAGCTAT+TGG - tig0020279:136065-136084 None:intergenic 40.0%
TTCTTCTTCTCCTTCGTCTG+AGG - tig0020279:134747-134766 None:intergenic 45.0%
AACTGCAACTGTTTGCGTTG+TGG + tig0020279:135149-135168 Msa1379500:intron 45.0%
! ATGATGGAAACCGCTTCAGT+TGG - tig0020279:135453-135472 None:intergenic 45.0%
ACTGAAGCGGTTTCCATCAT+TGG + tig0020279:135453-135472 Msa1379500:intron 45.0%
ATCCGTCACCAAAACTTGGA+TGG - tig0020279:135515-135534 None:intergenic 45.0%
GCAGATGAATCAAAGAAGGG+AGG + tig0020279:135752-135771 Msa1379500:CDS 45.0%
CATGTGTGTCCAATAGCTCT+AGG + tig0020279:136053-136072 Msa1379500:three_prime_UTR 45.0%
TTCTTCTTCTCCTTCGTCTG+AGG - tig0020279:134747-134766 None:intergenic 45.0%
AACTGCAACTGTTTGCGTTG+TGG + tig0020279:135149-135168 Msa1379500:intron 45.0%
! ATGATGGAAACCGCTTCAGT+TGG - tig0020279:135453-135472 None:intergenic 45.0%
ACTGAAGCGGTTTCCATCAT+TGG + tig0020279:135453-135472 Msa1379500:intron 45.0%
ATCCGTCACCAAAACTTGGA+TGG - tig0020279:135515-135534 None:intergenic 45.0%
GCAGATGAATCAAAGAAGGG+AGG + tig0020279:135752-135771 Msa1379500:CDS 45.0%
CATGTGTGTCCAATAGCTCT+AGG + tig0020279:136053-136072 Msa1379500:three_prime_UTR 45.0%
ATGGTATGCACCTCAGACGA+AGG + tig0020279:134734-134753 Msa1379500:CDS 50.0%
CCTAAACTAAGGGTGAGCCA+GGG - tig0020279:135094-135113 None:intergenic 50.0%
ACCTAAACTAAGGGTGAGCC+AGG - tig0020279:135095-135114 None:intergenic 50.0%
ATGGTATGCACCTCAGACGA+AGG + tig0020279:134734-134753 Msa1379500:CDS 50.0%
CCTAAACTAAGGGTGAGCCA+GGG - tig0020279:135094-135113 None:intergenic 50.0%
ACCTAAACTAAGGGTGAGCC+AGG - tig0020279:135095-135114 None:intergenic 50.0%
CCCTGGCTCACCCTTAGTTT+AGG + tig0020279:135091-135110 Msa1379500:intron 55.0%
CGTCTGGCCTACTGCCTAAA+TGG + tig0020279:135576-135595 Msa1379500:intron 55.0%
CCCTGGCTCACCCTTAGTTT+AGG + tig0020279:135091-135110 Msa1379500:intron 55.0%
CGTCTGGCCTACTGCCTAAA+TGG + tig0020279:135576-135595 Msa1379500:intron 55.0%
!!! GGCTTTTGGGATTGGGCGTC+TGG + tig0020279:135560-135579 Msa1379500:intron 60.0%
!!! GGCTTTTGGGATTGGGCGTC+TGG + tig0020279:135560-135579 Msa1379500:intron 60.0%
Chromosome Type Strat End Strand Name
tig0020279 gene 134734 136120 134734 ID=Msa1379500;Name=Msa1379500
tig0020279 mRNA 134734 136120 134734 ID=Msa1379500-mRNA-1;Parent=Msa1379500;Name=Msa1379500-mRNA-1;_AED=0.17;_eAED=0.30;_QI=0|0|0.5|1|0|0|2|285|65
tig0020279 exon 134734 134809 134734 ID=Msa1379500-mRNA-1:exon:6583;Parent=Msa1379500-mRNA-1
tig0020279 exon 135714 136120 135714 ID=Msa1379500-mRNA-1:exon:6584;Parent=Msa1379500-mRNA-1
tig0020279 CDS 134734 134809 134734 ID=Msa1379500-mRNA-1:cds;Parent=Msa1379500-mRNA-1
tig0020279 CDS 135714 135835 135714 ID=Msa1379500-mRNA-1:cds;Parent=Msa1379500-mRNA-1
tig0020279 three_prime_UTR 135836 136120 135836 ID=Msa1379500-mRNA-1:three_prime_utr;Parent=Msa1379500-mRNA-1
Gene Sequence

>Msa1379500

ATGGTATGCACCTCAGACGAAGGAGAAGAAGAAGAAGAAGATGGGATGCACTCCCATTTCAACCATGTTTTCGACCGGAAAACACGTATTGTATTTTGCAAGGGAATGAAGAAAGCAGATGAATCAAAGAAGGGAGGAAAAAAAATGCAAAGAAAGGCAAAGGGAAGAGCAATAAGAAATAAAGGTTTTAATTTATGA

Protein sequence

>Msa1379500

MVCTSDEGEEEEEDGMHSHFNHVFDRKTRIVFCKGMKKADESKKGGKKMQRKAKGRAIRNKGFNL*