AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003685


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003685.t1 MTR_4g071000 98.684 152 2 0 1 152 9 160 5.08e-108 304
MS.gene003685.t1 MTR_3g020660 89.333 150 16 0 1 150 9 158 2.68e-96 275
MS.gene003685.t1 MTR_3g020520 82.787 122 21 0 29 150 404 525 4.02e-66 209
MS.gene003685.t1 MTR_3g020640 72.593 135 30 1 16 150 1003 1130 5.19e-59 197
MS.gene003685.t1 MTR_3g020650 76.471 85 20 0 30 114 1028 1112 1.78e-37 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003685.t1 AT1G17880 86.014 143 18 2 1 142 9 150 3.27e-85 247
MS.gene003685.t1 AT1G73230 85.235 149 21 1 1 148 9 157 2.15e-80 235

Find 51 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTCAGGAGCCTGCTTC+TGG 0.192683 4.1:-32001069 None:intergenic
TGAGGAGGTCAATATCTTTA+AGG 0.219626 4.1:+32000363 MS.gene003685:CDS
TAATTTCTTACTCTTTGTTT+GGG 0.224790 4.1:-32000580 None:intergenic
GTAATTTCTTACTCTTTGTT+TGG 0.245420 4.1:-32000581 None:intergenic
CATTCACACCTATTCTCTTC+AGG 0.292092 4.1:-32000327 None:intergenic
ATTGTTCAGCTATCTTCTTC+AGG 0.297553 4.1:-32001045 None:intergenic
TTTCACTTCTACAGAGTTGC+AGG 0.308571 4.1:+32000887 MS.gene003685:intron
GTTCAAAGCACATACCTAAT+TGG 0.327309 4.1:-32000932 None:intergenic
GCTGAACCAGCACCTGCTTC+AGG 0.334925 4.1:-32001082 None:intergenic
GTCTCTCCTGGGACAAGCTC+TGG 0.335105 4.1:-32001136 None:intergenic
CCTAGCATTATCCACCAATT+AGG 0.352096 4.1:+32000918 MS.gene003685:CDS
TACCTTTGGGGTTTAAGAAC+TGG 0.359158 4.1:-32000399 None:intergenic
CGTCCATTGCTGCTAATACA+TGG 0.419485 4.1:+32000541 MS.gene003685:CDS
GCAGTCTCAAAAGTCTCTCC+TGG 0.431286 4.1:-32001148 None:intergenic
ATTCACACCTATTCTCTTCA+GGG 0.451011 4.1:-32000326 None:intergenic
TATTGACCTCCTCAATTTGT+GGG 0.452604 4.1:-32000354 None:intergenic
ATGGGCTGATATTACCTTTG+GGG 0.456873 4.1:-32000411 None:intergenic
CAGAAGCAGGCTCCTGAAGC+AGG 0.458056 4.1:+32001070 MS.gene003685:CDS
TCCTCCGGTTCGAACAGAAC+CGG 0.461978 4.1:-31999816 None:intergenic
CAGAGCACCCTGAAGAGAAT+AGG 0.468639 4.1:+32000319 MS.gene003685:CDS
ATAACAAATCTAACTATCCT+TGG 0.472981 4.1:-32001182 None:intergenic
TGTTTACCTCAGAAAGAAGA+AGG 0.477565 4.1:+32000258 MS.gene003685:intron
TCTGTTCGAACCGGAGGAAA+GGG 0.482236 4.1:+31999821 MS.gene003685:CDS
CAGGCTCCTGAAGCAGGTGC+TGG 0.494992 4.1:+32001076 MS.gene003685:CDS
AGCTGAACAATTCCAGAAGC+AGG 0.497000 4.1:+32001057 MS.gene003685:CDS
CCTAATTGGTGGATAATGCT+AGG 0.497579 4.1:-32000918 None:intergenic
GACTGCTGCTGAGGAGGCCA+AGG 0.501675 4.1:+32001165 MS.gene003685:CDS
ATATTGACCTCCTCAATTTG+TGG 0.501827 4.1:-32000355 None:intergenic
CAACAACTACAGATGACAAA+AGG 0.502653 4.1:+32000293 MS.gene003685:CDS
GATGTCCCAGAGCTTGTCCC+AGG 0.514432 4.1:+32001130 MS.gene003685:CDS
GACCTCCTCAATTTGTGGGA+TGG 0.524360 4.1:-32000350 None:intergenic
CATCTGTAGTTGTTGTCTTG+TGG 0.525038 4.1:-32000285 None:intergenic
TTCTGTTCGAACCGGAGGAA+AGG 0.525397 4.1:+31999820 MS.gene003685:CDS
GCTAATACATGGGTTGTTAG+TGG 0.526432 4.1:+32000552 MS.gene003685:CDS
TCTCTCCTGGGACAAGCTCT+GGG 0.528395 4.1:-32001135 None:intergenic
GAATGCCATCCCACAAATTG+AGG 0.534495 4.1:+32000345 MS.gene003685:CDS
AACCCATGTATTAGCAGCAA+TGG 0.539996 4.1:-32000544 None:intergenic
TCTGACGGTACCCTTTCCTC+CGG 0.545452 4.1:-31999831 None:intergenic
AATTTCTTACTCTTTGTTTG+GGG 0.553423 4.1:-32000579 None:intergenic
TGTAGTTGTTGTCTTGTGGA+CGG 0.561453 4.1:-32000281 None:intergenic
CTAATTGGTGGATAATGCTA+GGG 0.563275 4.1:-32000917 None:intergenic
ATGGCCGGTTCTGTTCGAAC+CGG 0.568678 4.1:+31999812 MS.gene003685:CDS
GCCGGTTCTGTTCGAACCGG+AGG 0.569814 4.1:+31999815 MS.gene003685:CDS
CAGTCTCAAAAGTCTCTCCT+GGG 0.576944 4.1:-32001147 None:intergenic
GTCCATTGCTGCTAATACAT+GGG 0.579320 4.1:+32000542 MS.gene003685:CDS
ATCCAGTTCTTAAACCCCAA+AGG 0.585033 4.1:+32000397 MS.gene003685:CDS
GAAAAGTTGATGAAGATGGC+CGG 0.594138 4.1:+31999797 None:intergenic
CAAAGCACATACCTAATTGG+TGG 0.599520 4.1:-32000929 None:intergenic
GAGGAAAGGGTACCGTCAGA+AGG 0.619370 4.1:+31999834 MS.gene003685:CDS
CATATAAATATACCTTCTGA+CGG 0.639530 4.1:-31999846 None:intergenic
TGCCATCCCACAAATTGAGG+AGG 0.645011 4.1:+32000348 MS.gene003685:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAATATTATTAAAAAAC+AGG + chr4.1:32001004-32001023 MS.gene003685:intron 10.0%
!!! ATTTATTCTTTTTATATGAT+AGG + chr4.1:32000446-32000465 MS.gene003685:intron 10.0%
!! AAAAACAAGTTATATTAGAA+TGG - chr4.1:31999888-31999907 None:intergenic 15.0%
!!! AATATTTTAGATATATCTGT+CGG + chr4.1:31999953-31999972 MS.gene003685:intron 15.0%
!!! GAAATTAATATGTCAATTTT+AGG - chr4.1:32000871-32000890 None:intergenic 15.0%
!! AAGAAACATTCTTTAAACAA+TGG - chr4.1:32000154-32000173 None:intergenic 20.0%
!! AATAAATGCTGGAAATATTA+TGG - chr4.1:32000433-32000452 None:intergenic 20.0%
!! AATACTTTAAATGAAAACTC+TGG + chr4.1:32000679-32000698 MS.gene003685:intron 20.0%
!! ATAAATGCTGGAAATATTAT+GGG - chr4.1:32000432-32000451 None:intergenic 20.0%
!! CAATAGATATATGAAGAAAA+AGG - chr4.1:32000609-32000628 None:intergenic 20.0%
!! CATATAAAAAGAATAAATGC+TGG - chr4.1:32000444-32000463 None:intergenic 20.0%
!! TTTATATGTGAAACTCATTA+TGG + chr4.1:31999861-31999880 MS.gene003685:intron 20.0%
!!! TAATTTCTTACTCTTTGTTT+GGG - chr4.1:32000583-32000602 None:intergenic 20.0%
! AAACAAAAGGCAGAAAATTA+CGG - chr4.1:32000243-32000262 None:intergenic 25.0%
! AATATTCAAAACGTTCAACT+TGG - chr4.1:32000994-32001013 None:intergenic 25.0%
! AGAAAACAATCAAAGCTTAA+TGG - chr4.1:32000509-32000528 None:intergenic 25.0%
! ATATTCAAAACGTTCAACTT+GGG - chr4.1:32000993-32001012 None:intergenic 25.0%
! CAATGCTATACAACTAATAT+TGG - chr4.1:31999921-31999940 None:intergenic 25.0%
! CATATAAATATACCTTCTGA+CGG - chr4.1:31999849-31999868 None:intergenic 25.0%
! GTAATTTCTTACTCTTTGTT+TGG - chr4.1:32000584-32000603 None:intergenic 25.0%
!! AATTTCTTACTCTTTGTTTG+GGG - chr4.1:32000582-32000601 None:intergenic 25.0%
!! ATTTTCCCACAATTTATGAA+CGG + chr4.1:32000791-32000810 MS.gene003685:intron 25.0%
!!! AACTTTTTCAAAGGTTTGTT+TGG + chr4.1:32000952-32000971 MS.gene003685:intron 25.0%
!!! TTATATTTTAGAGTACACCA+GGG + chr4.1:32000765-32000784 MS.gene003685:intron 25.0%
AAAAACAGGACCAGATAATT+TGG + chr4.1:32001018-32001037 MS.gene003685:intron 30.0%
AAAATGAACAGATTCAGCAT+AGG - chr4.1:32000727-32000746 None:intergenic 30.0%
AACAAAAGGCAGAAAATTAC+GGG - chr4.1:32000242-32000261 None:intergenic 30.0%
! ATATCTGTCGGTTGTTATTA+GGG + chr4.1:31999965-31999984 MS.gene003685:intron 30.0%
! ATCATTTTCTTCTTGTACAG+TGG - chr4.1:32001107-32001126 None:intergenic 30.0%
! TATATCTGTCGGTTGTTATT+AGG + chr4.1:31999964-31999983 MS.gene003685:intron 30.0%
! TCTGTTCATTTTGCAATATC+TGG + chr4.1:32000735-32000754 MS.gene003685:intron 30.0%
! TTTTCCCACAATTTATGAAC+GGG + chr4.1:32000792-32000811 MS.gene003685:intron 30.0%
!! TTCAGGTTTTCCAAATTATC+TGG - chr4.1:32001031-32001050 None:intergenic 30.0%
!! TTCTTTCTGAGGTAAACAAA+AGG - chr4.1:32000256-32000275 None:intergenic 30.0%
!!! ATCATGTGCTCTTTGTTTTT+GGG + chr4.1:32000825-32000844 MS.gene003685:intron 30.0%
!!! GTTATATTTTAGAGTACACC+AGG + chr4.1:32000764-32000783 MS.gene003685:intron 30.0%
!!! TATCATGTGCTCTTTGTTTT+TGG + chr4.1:32000824-32000843 MS.gene003685:intron 30.0%
!!! TGTGCTTTGAACTTTTTCAA+AGG + chr4.1:32000943-32000962 MS.gene003685:intron 30.0%
ACACACTTGAATATGCTCAA+GGG - chr4.1:32000059-32000078 None:intergenic 35.0%
ATATTGACCTCCTCAATTTG+TGG - chr4.1:32000358-32000377 None:intergenic 35.0%
ATTCACACCTATTCTCTTCA+GGG - chr4.1:32000329-32000348 None:intergenic 35.0%
ATTGTTCAGCTATCTTCTTC+AGG - chr4.1:32001048-32001067 None:intergenic 35.0%
CAACAACTACAGATGACAAA+AGG + chr4.1:32000293-32000312 MS.gene003685:CDS 35.0%
CAAGAATTCATGTCAGCATA+TGG - chr4.1:32000005-32000024 None:intergenic 35.0%
GTTCAAAGCACATACCTAAT+TGG - chr4.1:32000935-32000954 None:intergenic 35.0%
TATTGACCTCCTCAATTTGT+GGG - chr4.1:32000357-32000376 None:intergenic 35.0%
TCAGAGTAAAAGAAAGAACG+AGG - chr4.1:32000102-32000121 None:intergenic 35.0%
TGAGGAGGTCAATATCTTTA+AGG + chr4.1:32000363-32000382 MS.gene003685:CDS 35.0%
TGTTTACCTCAGAAAGAAGA+AGG + chr4.1:32000258-32000277 MS.gene003685:intron 35.0%
! AGGAATTGTGAAGTTTTGCT+TGG - chr4.1:32000082-32000101 None:intergenic 35.0%
! CTAATTGGTGGATAATGCTA+GGG - chr4.1:32000920-32000939 None:intergenic 35.0%
! TATGGGCTGATATTACCTTT+GGG - chr4.1:32000415-32000434 None:intergenic 35.0%
! TTATGGGCTGATATTACCTT+TGG - chr4.1:32000416-32000435 None:intergenic 35.0%
AACCCATGTATTAGCAGCAA+TGG - chr4.1:32000547-32000566 None:intergenic 40.0%
AATTGTGGGAAAATATGCCC+TGG - chr4.1:32000785-32000804 None:intergenic 40.0%
ATCCAGTTCTTAAACCCCAA+AGG + chr4.1:32000397-32000416 MS.gene003685:CDS 40.0%
CAAAGCACATACCTAATTGG+TGG - chr4.1:32000932-32000951 None:intergenic 40.0%
CACACTTGAATATGCTCAAG+GGG - chr4.1:32000058-32000077 None:intergenic 40.0%
CATCTGTAGTTGTTGTCTTG+TGG - chr4.1:32000288-32000307 None:intergenic 40.0%
CATTCACACCTATTCTCTTC+AGG - chr4.1:32000330-32000349 None:intergenic 40.0%
CCTAGCATTATCCACCAATT+AGG + chr4.1:32000918-32000937 MS.gene003685:CDS 40.0%
CTAGCCCGTTCATAAATTGT+GGG - chr4.1:32000799-32000818 None:intergenic 40.0%
GACACACTTGAATATGCTCA+AGG - chr4.1:32000060-32000079 None:intergenic 40.0%
GTCCATTGCTGCTAATACAT+GGG + chr4.1:32000542-32000561 MS.gene003685:CDS 40.0%
TCAAAACGTTCAACTTGGGT+AGG - chr4.1:32000989-32001008 None:intergenic 40.0%
TCTAGCCCGTTCATAAATTG+TGG - chr4.1:32000800-32000819 None:intergenic 40.0%
TGAATATGCTCAAGGGGAAT+GGG - chr4.1:32000052-32000071 None:intergenic 40.0%
TGTAGTTGTTGTCTTGTGGA+CGG - chr4.1:32000284-32000303 None:intergenic 40.0%
TTGAATATGCTCAAGGGGAA+TGG - chr4.1:32000053-32000072 None:intergenic 40.0%
TTTCACTTCTACAGAGTTGC+AGG + chr4.1:32000887-32000906 MS.gene003685:intron 40.0%
! ATGGGCTGATATTACCTTTG+GGG - chr4.1:32000414-32000433 None:intergenic 40.0%
! CCTAATTGGTGGATAATGCT+AGG - chr4.1:32000921-32000940 None:intergenic 40.0%
! GCTAATACATGGGTTGTTAG+TGG + chr4.1:32000552-32000571 MS.gene003685:CDS 40.0%
!! TACCTTTGGGGTTTAAGAAC+TGG - chr4.1:32000402-32000421 None:intergenic 40.0%
AGCTGAACAATTCCAGAAGC+AGG + chr4.1:32001057-32001076 MS.gene003685:CDS 45.0%
CAGTCTCAAAAGTCTCTCCT+GGG - chr4.1:32001150-32001169 None:intergenic 45.0%
CGTCCATTGCTGCTAATACA+TGG + chr4.1:32000541-32000560 MS.gene003685:CDS 45.0%
GAATATGCTCAAGGGGAATG+GGG - chr4.1:32000051-32000070 None:intergenic 45.0%
GAATGCCATCCCACAAATTG+AGG + chr4.1:32000345-32000364 MS.gene003685:CDS 45.0%
TTACGGGACAACTCAACTCT+TGG - chr4.1:32000226-32000245 None:intergenic 45.0%
GACCTCCTCAATTTGTGGGA+TGG - chr4.1:32000353-32000372 None:intergenic 50.0%
GCAGTCTCAAAAGTCTCTCC+TGG - chr4.1:32001151-32001170 None:intergenic 50.0%
TCTGTTCGAACCGGAGGAAA+GGG + chr4.1:31999821-31999840 MS.gene003685:CDS 50.0%
TGCCATCCCACAAATTGAGG+AGG + chr4.1:32000348-32000367 MS.gene003685:CDS 50.0%
TTCTGTTCGAACCGGAGGAA+AGG + chr4.1:31999820-31999839 MS.gene003685:CDS 50.0%
! CAGAGCACCCTGAAGAGAAT+AGG + chr4.1:32000319-32000338 MS.gene003685:CDS 50.0%
!!! GACTTTTGAGACTGCTGCTG+AGG + chr4.1:32001156-32001175 MS.gene003685:CDS 50.0%
!!! TTTTGAGACTGCTGCTGAGG+AGG + chr4.1:32001159-32001178 MS.gene003685:CDS 50.0%
GCAGCAATGGACGCTTGAAC+TGG - chr4.1:32000534-32000553 None:intergenic 55.0%
GGACGGCCTTCTTCTTTCTG+AGG - chr4.1:32000267-32000286 None:intergenic 55.0%
TCTCTCCTGGGACAAGCTCT+GGG - chr4.1:32001138-32001157 None:intergenic 55.0%
TCTGACGGTACCCTTTCCTC+CGG - chr4.1:31999834-31999853 None:intergenic 55.0%
! ATGGCCGGTTCTGTTCGAAC+CGG + chr4.1:31999812-31999831 MS.gene003685:CDS 55.0%
! TCCTCCGGTTCGAACAGAAC+CGG - chr4.1:31999819-31999838 None:intergenic 55.0%
!! GAGGAAAGGGTACCGTCAGA+AGG + chr4.1:31999834-31999853 MS.gene003685:CDS 55.0%
CAGAAGCAGGCTCCTGAAGC+AGG + chr4.1:32001070-32001089 MS.gene003685:CDS 60.0%
CTGCTTCAGGAGCCTGCTTC+TGG - chr4.1:32001072-32001091 None:intergenic 60.0%
GATGTCCCAGAGCTTGTCCC+AGG + chr4.1:32001130-32001149 MS.gene003685:CDS 60.0%
GCTGAACCAGCACCTGCTTC+AGG - chr4.1:32001085-32001104 None:intergenic 60.0%
GTCTCTCCTGGGACAAGCTC+TGG - chr4.1:32001139-32001158 None:intergenic 60.0%
CAGGCTCCTGAAGCAGGTGC+TGG + chr4.1:32001076-32001095 MS.gene003685:CDS 65.0%
GACTGCTGCTGAGGAGGCCA+AGG + chr4.1:32001165-32001184 MS.gene003685:CDS 65.0%
! GCCGGTTCTGTTCGAACCGG+AGG + chr4.1:31999815-31999834 MS.gene003685:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 31999812 32001195 31999812 ID=MS.gene003685
chr4.1 mRNA 31999812 32001195 31999812 ID=MS.gene003685.t1;Parent=MS.gene003685
chr4.1 exon 31999812 31999855 31999812 ID=MS.gene003685.t1.exon1;Parent=MS.gene003685.t1
chr4.1 CDS 31999812 31999855 31999812 ID=cds.MS.gene003685.t1;Parent=MS.gene003685.t1
chr4.1 exon 32000270 32000418 32000270 ID=MS.gene003685.t1.exon2;Parent=MS.gene003685.t1
chr4.1 CDS 32000270 32000418 32000270 ID=cds.MS.gene003685.t1;Parent=MS.gene003685.t1
chr4.1 exon 32000535 32000591 32000535 ID=MS.gene003685.t1.exon3;Parent=MS.gene003685.t1
chr4.1 CDS 32000535 32000591 32000535 ID=cds.MS.gene003685.t1;Parent=MS.gene003685.t1
chr4.1 exon 32000901 32000939 32000901 ID=MS.gene003685.t1.exon4;Parent=MS.gene003685.t1
chr4.1 CDS 32000901 32000939 32000901 ID=cds.MS.gene003685.t1;Parent=MS.gene003685.t1
chr4.1 exon 32001026 32001195 32001026 ID=MS.gene003685.t1.exon5;Parent=MS.gene003685.t1
chr4.1 CDS 32001026 32001195 32001026 ID=cds.MS.gene003685.t1;Parent=MS.gene003685.t1
Gene Sequence

>MS.gene003685

ATGGCCGGTTCTGTTCGAACCGGAGGAAAGGGTACCGTCAGAAGGTATATTTATATGTGAAACTCATTATGGTCCATTCTAATATAACTTGTTTTTTTTAGTGATGCCAATATTAGTTGTATAGCATTGTTTTGTTCTTCAAATATTTTAGATATATCTGTCGGTTGTTATTAGGGTCACTACTTACTAGCCATATGCTGACATGAATTCTTGAGCGTATATTAAAATTAAAGTTTCCCCATTCCCCTTGAGCATATTCAAGTGTGTCCAAGCAAAACTTCACAATTCCTCGTTCTTTCTTTTACTCTGAACTCATACTTTGTTCATGTTGTTGCTAGTCCATTGTTTAAAGAATGTTTCTTGAACACACATTATGCTTTTGTTGAGTTGAATTGTCTATTTTGTTTCTTACCAAGAGTTGAGTTGTCCCGTAATTTTCTGCCTTTTGTTTACCTCAGAAAGAAGAAGGCCGTCCACAAGACAACAACTACAGATGACAAAAGGCTTCAGAGCACCCTGAAGAGAATAGGTGTGAATGCCATCCCACAAATTGAGGAGGTCAATATCTTTAAGGATGATGTAGTTATCCAGTTCTTAAACCCCAAAGGTAATATCAGCCCATAATATTTCCAGCATTTATTCTTTTTATATGATAGGATGTATGCTATATATATTGCTTTTCAAATTTTGCTGACCATTAAGCTTTGATTGTTTTCTTTCCAGTTCAAGCGTCCATTGCTGCTAATACATGGGTTGTTAGTGGTGCTCCCCAAACAAAGAGTAAGAAATTACATCCTTTTTCTTCATATATCTATTGCTCTAGTCTATTATATGTGTTTTGCTAACATAATATGCATATTTTCAAAGAATACTTTAAATGAAAACTCTGGCTGCATCAATCATACATGAGCACCTATGCTGAATCTGTTCATTTTGCAATATCTGGATTTTTGTTATATTTTAGAGTACACCAGGGCATATTTTCCCACAATTTATGAACGGGCTAGACTTCTATCATGTGCTCTTTGTTTTTGGGCTGATTATTTACTGCTTTGTCCTAAAATTGACATATTAATTTCACTTCTACAGAGTTGCAGGATATACTCCCTAGCATTATCCACCAATTAGGTATGTGCTTTGAACTTTTTCAAAGGTTTGTTTGGTGTTTCTTGTTCCTACCCAAGTTGAACGTTTTGAATATTATTAAAAAACAGGACCAGATAATTTGGAAAACCTGAAGAAGATAGCTGAACAATTCCAGAAGCAGGCTCCTGAAGCAGGTGCTGGTTCAGCCACTGTACAAGAAGAAAATGATGATGATGTCCCAGAGCTTGTCCCAGGAGAGACTTTTGAGACTGCTGCTGAGGAGGCCAAGGATAGTTAG

Protein sequence

>MS.gene003685.t1

MAGSVRTGGKGTVRRKKKAVHKTTTTDDKRLQSTLKRIGVNAIPQIEEVNIFKDDVVIQFLNPKVQASIAANTWVVSGAPQTKKLQDILPSIIHQLGPDNLENLKKIAEQFQKQAPEAGAGSATVQEENDDDVPELVPGETFETAAEEAKDS