Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044693.t1 | XP_003598765.2 | 97.5 | 159 | 0 | 1 | 1 | 155 | 1 | 159 | 2.80E-75 | 291.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044693.t1 | Q9SMW7 | 81.5 | 157 | 23 | 3 | 1 | 152 | 1 | 156 | 1.0e-57 | 224.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044693.t1 | B7FLX9 | 97.5 | 159 | 0 | 1 | 1 | 155 | 1 | 159 | 2.1e-75 | 291.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044693 | MS.gene41902 | PPI |
| MS.gene044693 | MS.gene039827 | PPI |
| MS.gene044693 | MS.gene013940 | PPI |
| MS.gene044693 | MS.gene23317 | PPI |
| MS.gene044693 | MS.gene73089 | PPI |
| MS.gene044693 | MS.gene003688 | PPI |
| MS.gene044693 | MS.gene42247 | PPI |
| MS.gene23317 | MS.gene044693 | PPI |
| MS.gene044693 | MS.gene41901 | PPI |
| MS.gene044693 | MS.gene068093 | PPI |
| MS.gene044693 | MS.gene50742 | PPI |
| MS.gene003685 | MS.gene044693 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044693.t1 | MTR_3g020660 | 97.484 | 159 | 0 | 1 | 1 | 155 | 1 | 159 | 3.69e-109 | 307 |
| MS.gene044693.t1 | MTR_4g071000 | 86.709 | 158 | 17 | 1 | 1 | 154 | 1 | 158 | 2.96e-96 | 275 |
| MS.gene044693.t1 | MTR_3g020520 | 94.309 | 123 | 7 | 0 | 33 | 155 | 404 | 526 | 5.86e-76 | 235 |
| MS.gene044693.t1 | MTR_3g020640 | 85.185 | 135 | 13 | 1 | 21 | 155 | 1004 | 1131 | 6.91e-70 | 228 |
| MS.gene044693.t1 | MTR_3g020650 | 82.353 | 85 | 15 | 0 | 34 | 118 | 1028 | 1112 | 2.12e-40 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044693.t1 | AT1G17880 | 82.781 | 151 | 20 | 3 | 1 | 146 | 1 | 150 | 6.03e-82 | 239 |
| MS.gene044693.t1 | AT1G73230 | 83.439 | 157 | 21 | 2 | 1 | 152 | 1 | 157 | 2.95e-79 | 232 |
Find 43 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGGAAGTCAACATATTTA+AGG | 0.202032 | 3.4:-12898351 | MS.gene044693:CDS |
| AAATTTAGGGCCAGATAATT+TGG | 0.214129 | 3.4:-12897659 | MS.gene044693:intron |
| GTTGCATCAGCTCCTGCTTC+AGG | 0.258032 | 3.4:+12897595 | None:intergenic |
| CATCTATTGCTGCCAATACT+TGG | 0.316766 | 3.4:-12898201 | MS.gene044693:CDS |
| ATGTAAATCACATACCTAAT+TGG | 0.346928 | 3.4:+12897743 | None:intergenic |
| CAAAGCACTCTGAAGAGAAT+TGG | 0.364468 | 3.4:-12898395 | MS.gene044693:CDS |
| CCCAACATCATTCACCAATT+AGG | 0.370291 | 3.4:-12897757 | MS.gene044693:intron |
| GTCTCCCCGGGGACAAGATC+TGG | 0.417172 | 3.4:+12897541 | None:intergenic |
| CAGAAGCAGGCTCCTGAAGC+AGG | 0.445075 | 3.4:-12897607 | MS.gene044693:CDS |
| CCTAATTGGTGAATGATGTT+GGG | 0.465851 | 3.4:+12897757 | None:intergenic |
| AGCTGAGCAATTTCAGAAGC+AGG | 0.470262 | 3.4:-12897620 | MS.gene044693:CDS |
| AAATATGTTGACTTCCTCAA+TGG | 0.472125 | 3.4:+12898355 | None:intergenic |
| TCCACCGGTTCTAACAGAAC+CGG | 0.478108 | 3.4:+12898770 | None:intergenic |
| GCCAATACTTGGGTTGTTAG+CGG | 0.491455 | 3.4:-12898190 | MS.gene044693:CDS |
| CTGAAGAGAATTGGAGTGAA+TGG | 0.492121 | 3.4:-12898386 | MS.gene044693:CDS |
| GCTGTACCAGATCTTGTCCC+CGG | 0.495159 | 3.4:-12897547 | MS.gene044693:CDS |
| TCTGTTAGAACCGGTGGAAA+GGG | 0.501562 | 3.4:-12898765 | MS.gene044693:CDS |
| ATGTTGACTTCCTCAATGGC+AGG | 0.506965 | 3.4:+12898359 | None:intergenic |
| ACCGCTAACAACCCAAGTAT+TGG | 0.513678 | 3.4:+12898189 | None:intergenic |
| ATCTATTGCTGCCAATACTT+GGG | 0.514177 | 3.4:-12898200 | MS.gene044693:CDS |
| GCAGTTTCAAAGGTCTCCCC+GGG | 0.516727 | 3.4:+12897529 | None:intergenic |
| ACCTAATTGGTGAATGATGT+TGG | 0.517368 | 3.4:+12897756 | None:intergenic |
| CTCCTCAGCAGCAGTTTCAA+AGG | 0.517897 | 3.4:+12897519 | None:intergenic |
| CTGTACCAGATCTTGTCCCC+GGG | 0.519062 | 3.4:-12897546 | MS.gene044693:CDS |
| TGATGCAACTGCAGCACAAG+AGG | 0.526172 | 3.4:-12897581 | MS.gene044693:CDS |
| TTCTGTTAGAACCGGTGGAA+AGG | 0.527490 | 3.4:-12898766 | MS.gene044693:CDS |
| AGCAGTTTCAAAGGTCTCCC+CGG | 0.531544 | 3.4:+12897528 | None:intergenic |
| GCCGGTTCTGTTAGAACCGG+TGG | 0.539695 | 3.4:-12898771 | MS.gene044693:CDS |
| GACCTTTGAAACTGCTGCTG+AGG | 0.544100 | 3.4:-12897521 | MS.gene044693:CDS |
| GTGAATTAATATTACCTTTG+GGG | 0.551720 | 3.4:+12898303 | None:intergenic |
| ATGGCCGGTTCTGTTAGAAC+CGG | 0.553954 | 3.4:-12898774 | MS.gene044693:CDS |
| CGTCTGTAGTAGCTGTCTTG+TGG | 0.564591 | 3.4:+12898429 | None:intergenic |
| TGTAGAAAAGTTGATGAAGA+TGG | 0.565842 | 3.4:-12898793 | MS.gene044693:CDS |
| TCTGACTGTACCCTTTCCAC+CGG | 0.575776 | 3.4:+12898755 | None:intergenic |
| AACTGCTGCTGAGGAGACCA+AGG | 0.581530 | 3.4:-12897512 | MS.gene044693:CDS |
| ATTCAGTTTCTAAACCCCAA+AGG | 0.603569 | 3.4:-12898317 | MS.gene044693:intron |
| TGTTGACTTCCTCAATGGCA+GGG | 0.607118 | 3.4:+12898360 | None:intergenic |
| GAAAAGTTGATGAAGATGGC+CGG | 0.607204 | 3.4:-12898789 | MS.gene044693:CDS |
| ATATGCTTACTCTTTGTCTG+AGG | 0.617486 | 3.4:+12898163 | None:intergenic |
| GAATGGTATCCCTGCCATTG+AGG | 0.621892 | 3.4:-12898369 | MS.gene044693:CDS |
| CTAATTGGTGAATGATGTTG+GGG | 0.645637 | 3.4:+12897758 | None:intergenic |
| TGTACCAGATCTTGTCCCCG+GGG | 0.745014 | 3.4:-12897545 | MS.gene044693:CDS |
| CAGTTTCAAAGGTCTCCCCG+GGG | 0.788463 | 3.4:+12897530 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTCAAATAAAAAACAATTAT+AGG | + | chr3.4:12897742-12897761 | None:intergenic | 15.0% |
| !!! | AATCTTATATTCTTATTTCT+TGG | - | chr3.4:12898621-12898640 | MS.gene044693:intron | 15.0% |
| !!! | TTATTTCTTGGAAAAATTTA+GGG | - | chr3.4:12898633-12898652 | MS.gene044693:intron | 15.0% |
| !!! | AATTGTTTTTTATTTGAGTG+TGG | - | chr3.4:12897744-12897763 | MS.gene044693:intron | 20.0% |
| !!! | CTTATTTCTTGGAAAAATTT+AGG | - | chr3.4:12898632-12898651 | MS.gene044693:intron | 20.0% |
| !!! | TAGTTTTACGAAACAAAAAT+TGG | + | chr3.4:12897704-12897723 | None:intergenic | 20.0% |
| !!! | TGTTTGATAATGTATCATTA+AGG | + | chr3.4:12898186-12898205 | None:intergenic | 20.0% |
| ! | ACGTGAATTAATATTACCTT+TGG | + | chr3.4:12898007-12898026 | None:intergenic | 25.0% |
| ! | ACTAACTGTTAAACTTGAAT+TGG | - | chr3.4:12898069-12898088 | MS.gene044693:intron | 25.0% |
| ! | ATGTAAATCACATACCTAAT+TGG | + | chr3.4:12898565-12898584 | None:intergenic | 25.0% |
| ! | CGTGAATTAATATTACCTTT+GGG | + | chr3.4:12898006-12898025 | None:intergenic | 25.0% |
| ! | GAAATCAGATCAAAATCAAA+AGG | + | chr3.4:12898467-12898486 | None:intergenic | 25.0% |
| ! | GTGAATTAATATTACCTTTG+GGG | + | chr3.4:12898005-12898024 | None:intergenic | 25.0% |
| ! | TATCAATGTTCAGAAATTGT+AGG | - | chr3.4:12898319-12898338 | MS.gene044693:CDS | 25.0% |
| !! | AAATTTTGAACATTCCAACA+TGG | + | chr3.4:12898373-12898392 | None:intergenic | 25.0% |
| !! | TTCAGATTTTCCAAATTATC+TGG | + | chr3.4:12898659-12898678 | None:intergenic | 25.0% |
| AAAATGAGGAAAACAATGCA+TGG | + | chr3.4:12898419-12898438 | None:intergenic | 30.0% | |
| ATTGGAGTTTAAGAGATACA+TGG | + | chr3.4:12897686-12897705 | None:intergenic | 30.0% | |
| GAAGTCTCACAATTAAAATG+AGG | + | chr3.4:12898433-12898452 | None:intergenic | 30.0% | |
| GATTGCTAGTAGTACATTAA+TGG | + | chr3.4:12897617-12897636 | None:intergenic | 30.0% | |
| GCATCAGACAAAAATAATAC+AGG | + | chr3.4:12897772-12897791 | None:intergenic | 30.0% | |
| TGAGGAAGTCAACATATTTA+AGG | - | chr3.4:12897954-12897973 | MS.gene044693:intron | 30.0% | |
| ! | AAATTTAGGGCCAGATAATT+TGG | - | chr3.4:12898646-12898665 | MS.gene044693:intron | 30.0% |
| !! | AAATATGTTGACTTCCTCAA+TGG | + | chr3.4:12897953-12897972 | None:intergenic | 30.0% |
| !! | AGCCTTCTTCTTTCTAAAAT+TGG | + | chr3.4:12897854-12897873 | None:intergenic | 30.0% |
| !! | ATTTTGATCTGATTTCATGC+TGG | - | chr3.4:12898471-12898490 | MS.gene044693:intron | 30.0% |
| !! | TGCCAATTTTAGAAAGAAGA+AGG | - | chr3.4:12897849-12897868 | MS.gene044693:intron | 30.0% |
| !! | TGTAGAAAAGTTGATGAAGA+TGG | - | chr3.4:12897512-12897531 | MS.gene044693:CDS | 30.0% |
| ACATGGCACACATCATATAA+TGG | + | chr3.4:12898356-12898375 | None:intergenic | 35.0% | |
| ACCTAATTGGTGAATGATGT+TGG | + | chr3.4:12898552-12898571 | None:intergenic | 35.0% | |
| ATATGCTTACTCTTTGTCTG+AGG | + | chr3.4:12898145-12898164 | None:intergenic | 35.0% | |
| ATCTATTGCTGCCAATACTT+GGG | - | chr3.4:12898105-12898124 | MS.gene044693:intron | 35.0% | |
| ATTCAGTTTCTAAACCCCAA+AGG | - | chr3.4:12897988-12898007 | MS.gene044693:intron | 35.0% | |
| CCTAATTGGTGAATGATGTT+GGG | + | chr3.4:12898551-12898570 | None:intergenic | 35.0% | |
| CTAATTGGTGAATGATGTTG+GGG | + | chr3.4:12898550-12898569 | None:intergenic | 35.0% | |
| TTATATGATGTGTGCCATGT+TGG | - | chr3.4:12898356-12898375 | MS.gene044693:CDS | 35.0% | |
| ! | ATATCCTGCAACTCTGAAAA+AGG | + | chr3.4:12898524-12898543 | None:intergenic | 35.0% |
| ! | TATCCTGCAACTCTGAAAAA+GGG | + | chr3.4:12898523-12898542 | None:intergenic | 35.0% |
| CAACTGAAACCTTACCACTT+TGG | - | chr3.4:12897652-12897671 | MS.gene044693:CDS | 40.0% | |
| CATCTATTGCTGCCAATACT+TGG | - | chr3.4:12898104-12898123 | MS.gene044693:intron | 40.0% | |
| CCCAACATCATTCACCAATT+AGG | - | chr3.4:12898548-12898567 | MS.gene044693:intron | 40.0% | |
| CTGAAGAGAATTGGAGTGAA+TGG | - | chr3.4:12897919-12897938 | MS.gene044693:intron | 40.0% | |
| GAGGAAAACAATGCATGGTA+TGG | + | chr3.4:12898414-12898433 | None:intergenic | 40.0% | |
| ! | CAAAGCACTCTGAAGAGAAT+TGG | - | chr3.4:12897910-12897929 | MS.gene044693:intron | 40.0% |
| !! | GAAAAGTTGATGAAGATGGC+CGG | - | chr3.4:12897516-12897535 | MS.gene044693:CDS | 40.0% |
| !!! | TTTCCCTTTTTCAGAGTTGC+AGG | - | chr3.4:12898517-12898536 | MS.gene044693:intron | 40.0% |
| ACCGCTAACAACCCAAGTAT+TGG | + | chr3.4:12898119-12898138 | None:intergenic | 45.0% | |
| AGCTGAGCAATTTCAGAAGC+AGG | - | chr3.4:12898685-12898704 | MS.gene044693:intron | 45.0% | |
| TCTGTTAGAACCGGTGGAAA+GGG | - | chr3.4:12897540-12897559 | MS.gene044693:CDS | 45.0% | |
| TGAAACCTTACCACTTTGGC+TGG | - | chr3.4:12897656-12897675 | MS.gene044693:intron | 45.0% | |
| TTCTGTTAGAACCGGTGGAA+AGG | - | chr3.4:12897539-12897558 | MS.gene044693:CDS | 45.0% | |
| ! | GCCAATACTTGGGTTGTTAG+CGG | - | chr3.4:12898115-12898134 | MS.gene044693:intron | 45.0% |
| !! | ATGTTGACTTCCTCAATGGC+AGG | + | chr3.4:12897949-12897968 | None:intergenic | 45.0% |
| !! | TGTTGACTTCCTCAATGGCA+GGG | + | chr3.4:12897948-12897967 | None:intergenic | 45.0% |
| ACATGGATAGCCAGCCAAAG+TGG | + | chr3.4:12897669-12897688 | None:intergenic | 50.0% | |
| AGCAGTTTCAAAGGTCTCCC+CGG | + | chr3.4:12898780-12898799 | None:intergenic | 50.0% | |
| CGTCTGTAGTAGCTGTCTTG+TGG | + | chr3.4:12897879-12897898 | None:intergenic | 50.0% | |
| CTCCTCAGCAGCAGTTTCAA+AGG | + | chr3.4:12898789-12898808 | None:intergenic | 50.0% | |
| GAATGGTATCCCTGCCATTG+AGG | - | chr3.4:12897936-12897955 | MS.gene044693:intron | 50.0% | |
| GATAGCCAGCCAAAGTGGTA+AGG | + | chr3.4:12897664-12897683 | None:intergenic | 50.0% | |
| TCTGACTGTACCCTTTCCAC+CGG | + | chr3.4:12897553-12897572 | None:intergenic | 50.0% | |
| TGATGCAACTGCAGCACAAG+AGG | - | chr3.4:12898724-12898743 | MS.gene044693:intron | 50.0% | |
| ! | ATGGCCGGTTCTGTTAGAAC+CGG | - | chr3.4:12897531-12897550 | MS.gene044693:CDS | 50.0% |
| ! | GTGGAAAGGGTACAGTCAGA+AGG | - | chr3.4:12897553-12897572 | MS.gene044693:CDS | 50.0% |
| !! | GACCTTTGAAACTGCTGCTG+AGG | - | chr3.4:12898784-12898803 | MS.gene044693:CDS | 50.0% |
| !! | TCCACCGGTTCTAACAGAAC+CGG | + | chr3.4:12897538-12897557 | None:intergenic | 50.0% |
| AACTGCTGCTGAGGAGACCA+AGG | - | chr3.4:12898793-12898812 | MS.gene044693:CDS | 55.0% | |
| CAGTTTCAAAGGTCTCCCCG+GGG | + | chr3.4:12898778-12898797 | None:intergenic | 55.0% | |
| CTGTACCAGATCTTGTCCCC+GGG | - | chr3.4:12898759-12898778 | MS.gene044693:intron | 55.0% | |
| GCAGTTTCAAAGGTCTCCCC+GGG | + | chr3.4:12898779-12898798 | None:intergenic | 55.0% | |
| GCTGTACCAGATCTTGTCCC+CGG | - | chr3.4:12898758-12898777 | MS.gene044693:intron | 55.0% | |
| GTTGCATCAGCTCCTGCTTC+AGG | + | chr3.4:12898713-12898732 | None:intergenic | 55.0% | |
| TGTACCAGATCTTGTCCCCG+GGG | - | chr3.4:12898760-12898779 | MS.gene044693:intron | 55.0% | |
| CAGAAGCAGGCTCCTGAAGC+AGG | - | chr3.4:12898698-12898717 | MS.gene044693:intron | 60.0% | |
| ! | GCCGGTTCTGTTAGAACCGG+TGG | - | chr3.4:12897534-12897553 | MS.gene044693:CDS | 60.0% |
| GTCTCCCCGGGGACAAGATC+TGG | + | chr3.4:12898767-12898786 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 12897507 | 12898820 | 12897507 | ID=MS.gene044693 |
| chr3.4 | mRNA | 12897507 | 12898820 | 12897507 | ID=MS.gene044693.t1;Parent=MS.gene044693 |
| chr3.4 | exon | 12898761 | 12898820 | 12898761 | ID=MS.gene044693.t1.exon1;Parent=MS.gene044693.t1 |
| chr3.4 | CDS | 12898761 | 12898820 | 12898761 | ID=cds.MS.gene044693.t1;Parent=MS.gene044693.t1 |
| chr3.4 | exon | 12898318 | 12898462 | 12898318 | ID=MS.gene044693.t1.exon2;Parent=MS.gene044693.t1 |
| chr3.4 | CDS | 12898318 | 12898462 | 12898318 | ID=cds.MS.gene044693.t1;Parent=MS.gene044693.t1 |
| chr3.4 | exon | 12898173 | 12898229 | 12898173 | ID=MS.gene044693.t1.exon3;Parent=MS.gene044693.t1 |
| chr3.4 | CDS | 12898173 | 12898229 | 12898173 | ID=cds.MS.gene044693.t1;Parent=MS.gene044693.t1 |
| chr3.4 | exon | 12897758 | 12897796 | 12897758 | ID=MS.gene044693.t1.exon4;Parent=MS.gene044693.t1 |
| chr3.4 | CDS | 12897758 | 12897796 | 12897758 | ID=cds.MS.gene044693.t1;Parent=MS.gene044693.t1 |
| chr3.4 | exon | 12897507 | 12897673 | 12897507 | ID=MS.gene044693.t1.exon5;Parent=MS.gene044693.t1 |
| chr3.4 | CDS | 12897507 | 12897673 | 12897507 | ID=cds.MS.gene044693.t1;Parent=MS.gene044693.t1 |
| Gene Sequence |
| Protein sequence |
