AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50742


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50742.t1 MTR_4g071000 98.750 160 2 0 1 160 1 160 9.36e-115 322
MS.gene50742.t1 MTR_3g020660 89.241 158 17 0 1 158 1 158 1.39e-101 288
MS.gene50742.t1 MTR_3g020520 82.787 122 21 0 37 158 404 525 9.39e-66 209
MS.gene50742.t1 MTR_3g020640 72.593 135 30 1 24 158 1003 1130 1.35e-58 196
MS.gene50742.t1 MTR_3g020650 76.471 85 20 0 38 122 1028 1112 2.40e-37 135
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50742.t1 AT1G17880 86.755 151 18 2 1 150 1 150 1.15e-91 263
MS.gene50742.t1 AT1G73230 85.987 157 21 1 1 156 1 157 6.41e-87 251

Find 50 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTCAGGAGCCTGCTTC+TGG 0.192683 4.3:-34254424 None:intergenic
TGAGGAGGTCAATATCTTTA+AGG 0.219626 4.3:+34253718 MS.gene50742:CDS
TAATTTCTTACTCTTTGTTT+GGG 0.224790 4.3:-34253935 None:intergenic
GTAATTTCTTACTCTTTGTT+TGG 0.245420 4.3:-34253936 None:intergenic
CATTCACACCTATTCTCTTC+AGG 0.292092 4.3:-34253682 None:intergenic
ATTGTTCAGCTATCTTCTTC+AGG 0.297553 4.3:-34254400 None:intergenic
TTTCACTTCTACAGAGTTGC+AGG 0.308571 4.3:+34254242 MS.gene50742:intron
GTTCAAAGCACATACCTAAT+TGG 0.327309 4.3:-34254287 None:intergenic
GCTGAACCAGCACCTGCTTC+AGG 0.334925 4.3:-34254437 None:intergenic
GTCTCTCCTGGGACAAGCTC+TGG 0.335105 4.3:-34254491 None:intergenic
CCTAGCATTATCCACCAATT+AGG 0.352096 4.3:+34254273 MS.gene50742:CDS
TACCTTTGGGGTTTAAGAAC+TGG 0.359158 4.3:-34253754 None:intergenic
TCCTCCAGTTCGAACAGAAC+CGG 0.412678 4.3:-34253170 None:intergenic
CGTCCATTGCTGCTAATACA+TGG 0.419485 4.3:+34253896 MS.gene50742:CDS
GCTGTCTCAAAAGTCTCTCC+TGG 0.443808 4.3:-34254503 None:intergenic
ATTCACACCTATTCTCTTCA+GGG 0.451011 4.3:-34253681 None:intergenic
TATTGACCTCCTCAATTTGT+GGG 0.452604 4.3:-34253709 None:intergenic
ATGGGCTGATATTACCTTTG+GGG 0.456873 4.3:-34253766 None:intergenic
CAGAAGCAGGCTCCTGAAGC+AGG 0.458056 4.3:+34254425 MS.gene50742:CDS
CAGAGCACCCTGAAGAGAAT+AGG 0.468639 4.3:+34253674 MS.gene50742:CDS
ATAACAAATCTAACTATCCT+TGG 0.472981 4.3:-34254537 None:intergenic
TGTTTACCTCAGAAAGAAGA+AGG 0.477565 4.3:+34253613 MS.gene50742:intron
CAGGCTCCTGAAGCAGGTGC+TGG 0.494992 4.3:+34254431 MS.gene50742:CDS
AGCTGAACAATTCCAGAAGC+AGG 0.497000 4.3:+34254412 MS.gene50742:CDS
CCTAATTGGTGGATAATGCT+AGG 0.497579 4.3:-34254273 None:intergenic
ATATTGACCTCCTCAATTTG+TGG 0.501827 4.3:-34253710 None:intergenic
CAACAACTACAGATGACAAA+AGG 0.502653 4.3:+34253648 MS.gene50742:CDS
GATGTCCCAGAGCTTGTCCC+AGG 0.514432 4.3:+34254485 MS.gene50742:CDS
ATGGCCGGTTCTGTTCGAAC+TGG 0.516050 4.3:+34253166 MS.gene50742:CDS
GACCTCCTCAATTTGTGGGA+TGG 0.524360 4.3:-34253705 None:intergenic
CATCTGTAGTTGTTGTCTTG+TGG 0.525038 4.3:-34253640 None:intergenic
GCTAATACATGGGTTGTTAG+TGG 0.526432 4.3:+34253907 MS.gene50742:CDS
TCTCTCCTGGGACAAGCTCT+GGG 0.528395 4.3:-34254490 None:intergenic
GAATGCCATCCCACAAATTG+AGG 0.534495 4.3:+34253700 MS.gene50742:CDS
AACCCATGTATTAGCAGCAA+TGG 0.539996 4.3:-34253899 None:intergenic
GACAGCTGCTGAGGAGGCCA+AGG 0.540301 4.3:+34254520 MS.gene50742:CDS
TTCTGTTCGAACTGGAGGAA+AGG 0.541111 4.3:+34253174 MS.gene50742:CDS
TCTGTTCGAACTGGAGGAAA+GGG 0.543955 4.3:+34253175 MS.gene50742:CDS
GCCGGTTCTGTTCGAACTGG+AGG 0.553108 4.3:+34253169 MS.gene50742:CDS
AATTTCTTACTCTTTGTTTG+GGG 0.553423 4.3:-34253934 None:intergenic
TGTAGTTGTTGTCTTGTGGA+CGG 0.561453 4.3:-34253636 None:intergenic
CTAATTGGTGGATAATGCTA+GGG 0.563275 4.3:-34254272 None:intergenic
CTGTCTCAAAAGTCTCTCCT+GGG 0.571093 4.3:-34254502 None:intergenic
GTCCATTGCTGCTAATACAT+GGG 0.579320 4.3:+34253897 MS.gene50742:CDS
ATCCAGTTCTTAAACCCCAA+AGG 0.585033 4.3:+34253752 MS.gene50742:CDS
GAAAAGTTGATGAAGATGGC+CGG 0.594138 4.3:+34253151 MS.gene50742:CDS
TCGTGAAAAGTTGATGAAGA+TGG 0.598987 4.3:+34253147 MS.gene50742:CDS
CAAAGCACATACCTAATTGG+TGG 0.599520 4.3:-34254284 None:intergenic
GAGGAAAGGGTACTGTCAGA+AGG 0.636389 4.3:+34253188 MS.gene50742:CDS
TGCCATCCCACAAATTGAGG+AGG 0.645011 4.3:+34253703 MS.gene50742:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGAATATTATTAAAAAAC+AGG + chr4.3:34254359-34254378 MS.gene50742:intron 10.0%
!!! ATTTATTCTTTTTATATGAT+AGG + chr4.3:34253801-34253820 MS.gene50742:intron 10.0%
!! AAAAACAAGTTATATTAGAA+TGG - chr4.3:34253242-34253261 None:intergenic 15.0%
!!! AATATTTTAGATATATCTGT+CGG + chr4.3:34253307-34253326 MS.gene50742:intron 15.0%
!!! GAAATTAATATGTCAATTTT+AGG - chr4.3:34254226-34254245 None:intergenic 15.0%
!! AAGAAACATTCTTTAAACAA+TGG - chr4.3:34253507-34253526 None:intergenic 20.0%
!! AATAAATGCTGGAAATATTA+TGG - chr4.3:34253788-34253807 None:intergenic 20.0%
!! AATACTTTAAATGAAAACTC+TGG + chr4.3:34254034-34254053 MS.gene50742:intron 20.0%
!! ATAAATGCTGGAAATATTAT+GGG - chr4.3:34253787-34253806 None:intergenic 20.0%
!! CAATAGATATATGAAGAAAA+AGG - chr4.3:34253964-34253983 None:intergenic 20.0%
!! CATATAAAAAGAATAAATGC+TGG - chr4.3:34253799-34253818 None:intergenic 20.0%
!! TTTATATGTGAAACTCATTA+TGG + chr4.3:34253215-34253234 MS.gene50742:intron 20.0%
!!! TAATTTCTTACTCTTTGTTT+GGG - chr4.3:34253938-34253957 None:intergenic 20.0%
! AAACAAAAGGCAGAAAATTA+CGG - chr4.3:34253598-34253617 None:intergenic 25.0%
! AATATTCAAAACGTTCAACT+TGG - chr4.3:34254349-34254368 None:intergenic 25.0%
! AGAAAACAATCAAAGCTTAA+TGG - chr4.3:34253864-34253883 None:intergenic 25.0%
! ATATTCAAAACGTTCAACTT+GGG - chr4.3:34254348-34254367 None:intergenic 25.0%
! CAATGCTATACAACTAATAT+TGG - chr4.3:34253275-34253294 None:intergenic 25.0%
! GTAATTTCTTACTCTTTGTT+TGG - chr4.3:34253939-34253958 None:intergenic 25.0%
!! AATTTCTTACTCTTTGTTTG+GGG - chr4.3:34253937-34253956 None:intergenic 25.0%
!! ATTTTCCCACAATTTATGAA+CGG + chr4.3:34254146-34254165 MS.gene50742:intron 25.0%
!!! AACTTTTTCAAAGGTTTGTT+TGG + chr4.3:34254307-34254326 MS.gene50742:intron 25.0%
!!! TTATATTTTAGAGTACACCA+GGG + chr4.3:34254120-34254139 MS.gene50742:intron 25.0%
AAAAACAGGACCAGATAATT+TGG + chr4.3:34254373-34254392 MS.gene50742:intron 30.0%
AAAATGAACAGATTCAGCAT+AGG - chr4.3:34254082-34254101 None:intergenic 30.0%
AAACACTCGAATATGCTTAA+GGG - chr4.3:34253412-34253431 None:intergenic 30.0%
AACAAAAGGCAGAAAATTAC+GGG - chr4.3:34253597-34253616 None:intergenic 30.0%
! ATATCTGTCGGTTGTTATTA+GGG + chr4.3:34253319-34253338 MS.gene50742:intron 30.0%
! ATCATTTTCTTCTTGTACAG+TGG - chr4.3:34254462-34254481 None:intergenic 30.0%
! TATATCTGTCGGTTGTTATT+AGG + chr4.3:34253318-34253337 MS.gene50742:intron 30.0%
! TCTGTTCATTTTGCAATATC+TGG + chr4.3:34254090-34254109 MS.gene50742:intron 30.0%
! TTTTCCCACAATTTATGAAC+GGG + chr4.3:34254147-34254166 MS.gene50742:intron 30.0%
!! TTCAGGTTTTCCAAATTATC+TGG - chr4.3:34254386-34254405 None:intergenic 30.0%
!! TTCTTTCTGAGGTAAACAAA+AGG - chr4.3:34253611-34253630 None:intergenic 30.0%
!!! ATCATGTGCTCTTTGTTTTT+GGG + chr4.3:34254180-34254199 MS.gene50742:intron 30.0%
!!! GTTATATTTTAGAGTACACC+AGG + chr4.3:34254119-34254138 MS.gene50742:intron 30.0%
!!! TATCATGTGCTCTTTGTTTT+TGG + chr4.3:34254179-34254198 MS.gene50742:intron 30.0%
!!! TGTGCTTTGAACTTTTTCAA+AGG + chr4.3:34254298-34254317 MS.gene50742:intron 30.0%
AACACTCGAATATGCTTAAG+GGG - chr4.3:34253411-34253430 None:intergenic 35.0%
ATATTGACCTCCTCAATTTG+TGG - chr4.3:34253713-34253732 None:intergenic 35.0%
ATTCACACCTATTCTCTTCA+GGG - chr4.3:34253684-34253703 None:intergenic 35.0%
ATTGTTCAGCTATCTTCTTC+AGG - chr4.3:34254403-34254422 None:intergenic 35.0%
CAACAACTACAGATGACAAA+AGG + chr4.3:34253648-34253667 MS.gene50742:CDS 35.0%
CAAGAATTCATGTCAGCATA+TGG - chr4.3:34253359-34253378 None:intergenic 35.0%
GAAACACTCGAATATGCTTA+AGG - chr4.3:34253413-34253432 None:intergenic 35.0%
GTTCAAAGCACATACCTAAT+TGG - chr4.3:34254290-34254309 None:intergenic 35.0%
TATTGACCTCCTCAATTTGT+GGG - chr4.3:34253712-34253731 None:intergenic 35.0%
TCAGAGTAAAAGAAAGAACG+AGG - chr4.3:34253455-34253474 None:intergenic 35.0%
TGAGGAGGTCAATATCTTTA+AGG + chr4.3:34253718-34253737 MS.gene50742:CDS 35.0%
TGTTTACCTCAGAAAGAAGA+AGG + chr4.3:34253613-34253632 MS.gene50742:intron 35.0%
! AGGAATTGTGAAGTTTTGCT+TGG - chr4.3:34253435-34253454 None:intergenic 35.0%
! CTAATTGGTGGATAATGCTA+GGG - chr4.3:34254275-34254294 None:intergenic 35.0%
! TATGGGCTGATATTACCTTT+GGG - chr4.3:34253770-34253789 None:intergenic 35.0%
! TTATGGGCTGATATTACCTT+TGG - chr4.3:34253771-34253790 None:intergenic 35.0%
!! TCGTGAAAAGTTGATGAAGA+TGG + chr4.3:34253147-34253166 MS.gene50742:CDS 35.0%
AACCCATGTATTAGCAGCAA+TGG - chr4.3:34253902-34253921 None:intergenic 40.0%
AATTGTGGGAAAATATGCCC+TGG - chr4.3:34254140-34254159 None:intergenic 40.0%
ATCCAGTTCTTAAACCCCAA+AGG + chr4.3:34253752-34253771 MS.gene50742:CDS 40.0%
CAAAGCACATACCTAATTGG+TGG - chr4.3:34254287-34254306 None:intergenic 40.0%
CATCTGTAGTTGTTGTCTTG+TGG - chr4.3:34253643-34253662 None:intergenic 40.0%
CATTCACACCTATTCTCTTC+AGG - chr4.3:34253685-34253704 None:intergenic 40.0%
CCTAGCATTATCCACCAATT+AGG + chr4.3:34254273-34254292 MS.gene50742:CDS 40.0%
CGAATATGCTTAAGGGGAAT+GGG - chr4.3:34253405-34253424 None:intergenic 40.0%
CTAGCCCGTTCATAAATTGT+GGG - chr4.3:34254154-34254173 None:intergenic 40.0%
GAATATGCTTAAGGGGAATG+GGG - chr4.3:34253404-34253423 None:intergenic 40.0%
GTCCATTGCTGCTAATACAT+GGG + chr4.3:34253897-34253916 MS.gene50742:CDS 40.0%
TCAAAACGTTCAACTTGGGT+AGG - chr4.3:34254344-34254363 None:intergenic 40.0%
TCGAATATGCTTAAGGGGAA+TGG - chr4.3:34253406-34253425 None:intergenic 40.0%
TCTAGCCCGTTCATAAATTG+TGG - chr4.3:34254155-34254174 None:intergenic 40.0%
TGTAGTTGTTGTCTTGTGGA+CGG - chr4.3:34253639-34253658 None:intergenic 40.0%
TTTCACTTCTACAGAGTTGC+AGG + chr4.3:34254242-34254261 MS.gene50742:intron 40.0%
! ATGGGCTGATATTACCTTTG+GGG - chr4.3:34253769-34253788 None:intergenic 40.0%
! CCTAATTGGTGGATAATGCT+AGG - chr4.3:34254276-34254295 None:intergenic 40.0%
! GCTAATACATGGGTTGTTAG+TGG + chr4.3:34253907-34253926 MS.gene50742:CDS 40.0%
!! GAAAAGTTGATGAAGATGGC+CGG + chr4.3:34253151-34253170 MS.gene50742:CDS 40.0%
!! TACCTTTGGGGTTTAAGAAC+TGG - chr4.3:34253757-34253776 None:intergenic 40.0%
AGCTGAACAATTCCAGAAGC+AGG + chr4.3:34254412-34254431 MS.gene50742:CDS 45.0%
CGTCCATTGCTGCTAATACA+TGG + chr4.3:34253896-34253915 MS.gene50742:CDS 45.0%
CTGTCTCAAAAGTCTCTCCT+GGG - chr4.3:34254505-34254524 None:intergenic 45.0%
GAATGCCATCCCACAAATTG+AGG + chr4.3:34253700-34253719 MS.gene50742:CDS 45.0%
TCTGTTCGAACTGGAGGAAA+GGG + chr4.3:34253175-34253194 MS.gene50742:CDS 45.0%
TTACGGGACAACTCAACTCT+TGG - chr4.3:34253581-34253600 None:intergenic 45.0%
TTCTGTTCGAACTGGAGGAA+AGG + chr4.3:34253174-34253193 MS.gene50742:CDS 45.0%
GACCTCCTCAATTTGTGGGA+TGG - chr4.3:34253708-34253727 None:intergenic 50.0%
GCTGTCTCAAAAGTCTCTCC+TGG - chr4.3:34254506-34254525 None:intergenic 50.0%
TCCTCCAGTTCGAACAGAAC+CGG - chr4.3:34253173-34253192 None:intergenic 50.0%
TGCCATCCCACAAATTGAGG+AGG + chr4.3:34253703-34253722 MS.gene50742:CDS 50.0%
! CAGAGCACCCTGAAGAGAAT+AGG + chr4.3:34253674-34253693 MS.gene50742:CDS 50.0%
!! GAGGAAAGGGTACTGTCAGA+AGG + chr4.3:34253188-34253207 MS.gene50742:CDS 50.0%
!!! GACTTTTGAGACAGCTGCTG+AGG + chr4.3:34254511-34254530 MS.gene50742:CDS 50.0%
!!! TTTTGAGACAGCTGCTGAGG+AGG + chr4.3:34254514-34254533 MS.gene50742:CDS 50.0%
GCAGCAATGGACGCTTGAAC+TGG - chr4.3:34253889-34253908 None:intergenic 55.0%
GGACGGCCTTCTTCTTTCTG+AGG - chr4.3:34253622-34253641 None:intergenic 55.0%
TCTCTCCTGGGACAAGCTCT+GGG - chr4.3:34254493-34254512 None:intergenic 55.0%
! ATGGCCGGTTCTGTTCGAAC+TGG + chr4.3:34253166-34253185 MS.gene50742:CDS 55.0%
CAGAAGCAGGCTCCTGAAGC+AGG + chr4.3:34254425-34254444 MS.gene50742:CDS 60.0%
CTGCTTCAGGAGCCTGCTTC+TGG - chr4.3:34254427-34254446 None:intergenic 60.0%
GATGTCCCAGAGCTTGTCCC+AGG + chr4.3:34254485-34254504 MS.gene50742:CDS 60.0%
GCTGAACCAGCACCTGCTTC+AGG - chr4.3:34254440-34254459 None:intergenic 60.0%
GTCTCTCCTGGGACAAGCTC+TGG - chr4.3:34254494-34254513 None:intergenic 60.0%
! GCCGGTTCTGTTCGAACTGG+AGG + chr4.3:34253169-34253188 MS.gene50742:CDS 60.0%
CAGGCTCCTGAAGCAGGTGC+TGG + chr4.3:34254431-34254450 MS.gene50742:CDS 65.0%
GACAGCTGCTGAGGAGGCCA+AGG + chr4.3:34254520-34254539 MS.gene50742:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 34253142 34254550 34253142 ID=MS.gene50742
chr4.3 mRNA 34253142 34254550 34253142 ID=MS.gene50742.t1;Parent=MS.gene50742
chr4.3 exon 34253142 34253209 34253142 ID=MS.gene50742.t1.exon1;Parent=MS.gene50742.t1
chr4.3 CDS 34253142 34253209 34253142 ID=cds.MS.gene50742.t1;Parent=MS.gene50742.t1
chr4.3 exon 34253625 34253773 34253625 ID=MS.gene50742.t1.exon2;Parent=MS.gene50742.t1
chr4.3 CDS 34253625 34253773 34253625 ID=cds.MS.gene50742.t1;Parent=MS.gene50742.t1
chr4.3 exon 34253890 34253946 34253890 ID=MS.gene50742.t1.exon3;Parent=MS.gene50742.t1
chr4.3 CDS 34253890 34253946 34253890 ID=cds.MS.gene50742.t1;Parent=MS.gene50742.t1
chr4.3 exon 34254256 34254294 34254256 ID=MS.gene50742.t1.exon4;Parent=MS.gene50742.t1
chr4.3 CDS 34254256 34254294 34254256 ID=cds.MS.gene50742.t1;Parent=MS.gene50742.t1
chr4.3 exon 34254381 34254550 34254381 ID=MS.gene50742.t1.exon5;Parent=MS.gene50742.t1
chr4.3 CDS 34254381 34254550 34254381 ID=cds.MS.gene50742.t1;Parent=MS.gene50742.t1
Gene Sequence

>MS.gene50742

ATGAATCGTGAAAAGTTGATGAAGATGGCCGGTTCTGTTCGAACTGGAGGAAAGGGTACTGTCAGAAGGTATATTTATATGTGAAACTCATTATGGTCCATTCTAATATAACTTGTTTTTTTTAGTGATGCCAATATTAGTTGTATAGCATTGTTTTGTTCTTCAAATATTTTAGATATATCTGTCGGTTGTTATTAGGGTCACTACTTACTAGCCATATGCTGACATGAATTCTTGAGCGTATATTAAATTAAAGTTTCCCCATTCCCCTTAAGCATATTCGAGTGTTTCCAAGCAAAACTTCACAATTCCTCGTTCTTTCTTTTACTCTGAACTCATACTTTGTTCATGTTGTTGCAAATCCATTGTTTAAAGAATGTTTCTTGAACACACATTATGCTTTTGTTGAGTTGAATTGTCTATTTTGTTTTTCTTACCAAGAGTTGAGTTGTCCCGTAATTTTCTGCCTTTTGTTTACCTCAGAAAGAAGAAGGCCGTCCACAAGACAACAACTACAGATGACAAAAGGCTTCAGAGCACCCTGAAGAGAATAGGTGTGAATGCCATCCCACAAATTGAGGAGGTCAATATCTTTAAGGATGATGTAGTTATCCAGTTCTTAAACCCCAAAGGTAATATCAGCCCATAATATTTCCAGCATTTATTCTTTTTATATGATAGGATGTATGCTATATATATTGCTTTTCAAATTTTGCTGACCATTAAGCTTTGATTGTTTTCTTTCCAGTTCAAGCGTCCATTGCTGCTAATACATGGGTTGTTAGTGGTGCTCCCCAAACAAAGAGTAAGAAATTACATCCTTTTTCTTCATATATCTATTGCTCTAGTCTATTATATGTGTTTTGCTAACATAATATGCATATTTTCAAAGAATACTTTAAATGAAAACTCTGGCTGCATCAATCATACATGAGCACCTATGCTGAATCTGTTCATTTTGCAATATCTGGATTTTTGTTATATTTTAGAGTACACCAGGGCATATTTTCCCACAATTTATGAACGGGCTAGACTTCTATCATGTGCTCTTTGTTTTTGGGCTGATTATTTACTGCTTTGTCCTAAAATTGACATATTAATTTCACTTCTACAGAGTTGCAGGATATACTCCCTAGCATTATCCACCAATTAGGTATGTGCTTTGAACTTTTTCAAAGGTTTGTTTGGTGTTTCTTGTTCCTACCCAAGTTGAACGTTTTGAATATTATTAAAAAACAGGACCAGATAATTTGGAAAACCTGAAGAAGATAGCTGAACAATTCCAGAAGCAGGCTCCTGAAGCAGGTGCTGGTTCAGCCACTGTACAAGAAGAAAATGATGATGATGTCCCAGAGCTTGTCCCAGGAGAGACTTTTGAGACAGCTGCTGAGGAGGCCAAGGATAGTTAG

Protein sequence

>MS.gene50742.t1

MNREKLMKMAGSVRTGGKGTVRRKKKAVHKTTTTDDKRLQSTLKRIGVNAIPQIEEVNIFKDDVVIQFLNPKVQASIAANTWVVSGAPQTKKLQDILPSIIHQLGPDNLENLKKIAEQFQKQAPEAGAGSATVQEENDDDVPELVPGETFETAAEEAKDS