Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73089.t1 | XP_003598765.2 | 100 | 159 | 0 | 0 | 1 | 159 | 1 | 159 | 7.40E-79 | 303.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73089.t1 | Q9SMW7 | 84.1 | 157 | 23 | 2 | 1 | 156 | 1 | 156 | 3.5e-61 | 235.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73089.t1 | B7FLX9 | 100.0 | 159 | 0 | 0 | 1 | 159 | 1 | 159 | 5.3e-79 | 303.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene23317 | MS.gene73089 | PPI |
| MS.gene003685 | MS.gene73089 | PPI |
| MS.gene73089 | MS.gene50742 | PPI |
| MS.gene73089 | MS.gene41902 | PPI |
| MS.gene044693 | MS.gene73089 | PPI |
| MS.gene73089 | MS.gene039827 | PPI |
| MS.gene73089 | MS.gene003688 | PPI |
| MS.gene73089 | MS.gene42247 | PPI |
| MS.gene73089 | MS.gene013940 | PPI |
| MS.gene068093 | MS.gene73089 | PPI |
| MS.gene41901 | MS.gene73089 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73089.t1 | MTR_3g020660 | 100.000 | 159 | 0 | 0 | 1 | 159 | 1 | 159 | 1.08e-114 | 322 |
| MS.gene73089.t1 | MTR_4g071000 | 89.241 | 158 | 17 | 0 | 1 | 158 | 1 | 158 | 1.57e-101 | 288 |
| MS.gene73089.t1 | MTR_3g020520 | 94.309 | 123 | 7 | 0 | 37 | 159 | 404 | 526 | 6.24e-76 | 235 |
| MS.gene73089.t1 | MTR_3g020640 | 84.559 | 136 | 14 | 1 | 24 | 159 | 1003 | 1131 | 4.66e-70 | 229 |
| MS.gene73089.t1 | MTR_3g020650 | 82.353 | 85 | 15 | 0 | 38 | 122 | 1028 | 1112 | 2.46e-40 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73089.t1 | AT1G17880 | 85.430 | 151 | 20 | 2 | 1 | 150 | 1 | 150 | 2.76e-87 | 253 |
| MS.gene73089.t1 | AT1G73230 | 85.987 | 157 | 21 | 1 | 1 | 156 | 1 | 157 | 1.17e-84 | 246 |
Find 44 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGCATCAGCTCCTGCTTC+AGG | 0.258032 | 3.2:+10550122 | None:intergenic |
| CATCTATTGCTGCCAATACT+TGG | 0.316766 | 3.2:-10550732 | MS.gene73089:CDS |
| ATGTAAATCACATACCTAAT+TGG | 0.346928 | 3.2:+10550274 | None:intergenic |
| CAAAGCACTCTGAAGAGAAT+TGG | 0.364468 | 3.2:-10550973 | MS.gene73089:CDS |
| CCCAACATCATTCACCAATT+AGG | 0.370291 | 3.2:-10550288 | MS.gene73089:intron |
| GTCTCCCCGGGGACAAGATC+TGG | 0.417172 | 3.2:+10550068 | None:intergenic |
| AAATGTAGGGCCAGATAACT+TGG | 0.425878 | 3.2:-10550186 | MS.gene73089:intron |
| CAGAAGCAGGCTCCTGAAGC+AGG | 0.445075 | 3.2:-10550134 | MS.gene73089:CDS |
| CCTAATTGGTGAATGATGTT+GGG | 0.465851 | 3.2:+10550288 | None:intergenic |
| AGCTGAGCAATTTCAGAAGC+AGG | 0.470262 | 3.2:-10550147 | MS.gene73089:CDS |
| TCCACCGGTTCTAACAGAAC+CGG | 0.478108 | 3.2:+10551427 | None:intergenic |
| GAAGATGTTGACTTCCTCAA+TGG | 0.490121 | 3.2:+10550933 | None:intergenic |
| GCCAATACTTGGGTTGTTAG+CGG | 0.491455 | 3.2:-10550721 | MS.gene73089:CDS |
| CTGAAGAGAATTGGAGTGAA+TGG | 0.492121 | 3.2:-10550964 | MS.gene73089:CDS |
| GCTGTACCAGATCTTGTCCC+CGG | 0.495159 | 3.2:-10550074 | MS.gene73089:CDS |
| TCTGTTAGAACCGGTGGAAA+GGG | 0.501562 | 3.2:-10551422 | MS.gene73089:CDS |
| ACCGCTAACAACCCAAGTAT+TGG | 0.513678 | 3.2:+10550720 | None:intergenic |
| ATCTATTGCTGCCAATACTT+GGG | 0.514177 | 3.2:-10550731 | MS.gene73089:CDS |
| ATGTTGACTTCCTCAATGGC+AGG | 0.514214 | 3.2:+10550937 | None:intergenic |
| GCAGTTTCAAAGGTCTCCCC+GGG | 0.516727 | 3.2:+10550056 | None:intergenic |
| ACCTAATTGGTGAATGATGT+TGG | 0.517368 | 3.2:+10550287 | None:intergenic |
| CTCCTCAGCAGCAGTTTCAA+AGG | 0.517897 | 3.2:+10550046 | None:intergenic |
| CTGTACCAGATCTTGTCCCC+GGG | 0.519062 | 3.2:-10550073 | MS.gene73089:CDS |
| TGAGGAAGTCAACATCTTCA+AGG | 0.519088 | 3.2:-10550929 | MS.gene73089:CDS |
| TGATGCAACTGCAGCACAAG+AGG | 0.526172 | 3.2:-10550108 | MS.gene73089:CDS |
| TTCTGTTAGAACCGGTGGAA+AGG | 0.527490 | 3.2:-10551423 | MS.gene73089:CDS |
| AGCAGTTTCAAAGGTCTCCC+CGG | 0.531544 | 3.2:+10550055 | None:intergenic |
| GTGAATAAATATTACCTTTG+GGG | 0.533113 | 3.2:+10550881 | None:intergenic |
| GCCGGTTCTGTTAGAACCGG+TGG | 0.539695 | 3.2:-10551428 | MS.gene73089:CDS |
| GACCTTTGAAACTGCTGCTG+AGG | 0.544100 | 3.2:-10550048 | MS.gene73089:CDS |
| ATGGCCGGTTCTGTTAGAAC+CGG | 0.553954 | 3.2:-10551431 | MS.gene73089:CDS |
| CGTCTGTAGTAGCTGTCTTG+TGG | 0.564591 | 3.2:+10551007 | None:intergenic |
| TGTAGAAAAGTTGATGAAGA+TGG | 0.565842 | 3.2:-10551450 | MS.gene73089:CDS |
| TCTGACTGTACCCTTTCCAC+CGG | 0.575776 | 3.2:+10551412 | None:intergenic |
| AACTGCTGCTGAGGAGACCA+AGG | 0.581530 | 3.2:-10550039 | MS.gene73089:CDS |
| ATTCAGTTTCTAAACCCCAA+AGG | 0.603569 | 3.2:-10550895 | MS.gene73089:intron |
| GAAAAGTTGATGAAGATGGC+CGG | 0.607204 | 3.2:-10551446 | MS.gene73089:CDS |
| ATATGCTTACTCTTTGTCTG+AGG | 0.617486 | 3.2:+10550694 | None:intergenic |
| GAATGGTATCCCTGCCATTG+AGG | 0.621892 | 3.2:-10550947 | MS.gene73089:CDS |
| TGTTGACTTCCTCAATGGCA+GGG | 0.633833 | 3.2:+10550938 | None:intergenic |
| GTGGAAAGGGTACAGTCAGA+AGG | 0.638872 | 3.2:-10551409 | MS.gene73089:intron |
| CTAATTGGTGAATGATGTTG+GGG | 0.645637 | 3.2:+10550289 | None:intergenic |
| TGTACCAGATCTTGTCCCCG+GGG | 0.745014 | 3.2:-10550072 | MS.gene73089:CDS |
| CAGTTTCAAAGGTCTCCCCG+GGG | 0.788463 | 3.2:+10550057 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATCTTATATTCTTATTTCT+TGG | - | chr3.2:10551278-10551297 | MS.gene73089:intron | 15.0% |
| !!! | AAATTTTGAACATTCCAATA+TGG | + | chr3.2:10551026-10551045 | None:intergenic | 20.0% |
| !!! | TGTTTGATAATGTATCATTA+AGG | + | chr3.2:10550839-10550858 | None:intergenic | 20.0% |
| !!! | TTAAGAACATAACTTTTACA+AGG | + | chr3.2:10550140-10550159 | None:intergenic | 20.0% |
| !!! | TTATTTCTTGGAAAAATGTA+GGG | - | chr3.2:10551290-10551309 | MS.gene73089:intron | 20.0% |
| ! | ACTAACTGTTAAACTTGAAT+TGG | - | chr3.2:10550722-10550741 | MS.gene73089:CDS | 25.0% |
| ! | ATGTAAATCACATACCTAAT+TGG | + | chr3.2:10551218-10551237 | None:intergenic | 25.0% |
| ! | CGTGAATAAATATTACCTTT+GGG | + | chr3.2:10550612-10550631 | None:intergenic | 25.0% |
| ! | GTGAATAAATATTACCTTTG+GGG | + | chr3.2:10550611-10550630 | None:intergenic | 25.0% |
| ! | TCGTGAATAAATATTACCTT+TGG | + | chr3.2:10550613-10550632 | None:intergenic | 25.0% |
| !! | ATTTGAGTTTAAGAGATACA+TGG | + | chr3.2:10550292-10550311 | None:intergenic | 25.0% |
| !! | CTTATTTCTTGGAAAAATGT+AGG | - | chr3.2:10551289-10551308 | MS.gene73089:intron | 25.0% |
| !!! | AATTGTGTTTTATTTGAGTG+TGG | - | chr3.2:10550350-10550369 | MS.gene73089:intron | 25.0% |
| AAAATGAGGAAAACAATGCA+TGG | + | chr3.2:10551072-10551091 | None:intergenic | 30.0% | |
| CATCAATGTTCAGAAATTGT+AGG | - | chr3.2:10550972-10550991 | MS.gene73089:CDS | 30.0% | |
| GATTGCTAGTAGTACATTAA+TGG | + | chr3.2:10550189-10550208 | None:intergenic | 30.0% | |
| GTATCAGACAGAATTAATAC+AGG | + | chr3.2:10550378-10550397 | None:intergenic | 30.0% | |
| TGTTTGCAAAGAGAAAGATA+AGG | + | chr3.2:10550244-10550263 | None:intergenic | 30.0% | |
| ! | TTCAGATTTTCCAAGTTATC+TGG | + | chr3.2:10551316-10551335 | None:intergenic | 30.0% |
| !! | ATTTTGATCTGATTTCATGC+TGG | - | chr3.2:10551124-10551143 | MS.gene73089:intron | 30.0% |
| !! | TGCTCATTTTAGAAAGAAGA+AGG | - | chr3.2:10550455-10550474 | MS.gene73089:intron | 30.0% |
| !! | TGTAGAAAAGTTGATGAAGA+TGG | - | chr3.2:10550039-10550058 | MS.gene73089:CDS | 30.0% |
| ACCTAATTGGTGAATGATGT+TGG | + | chr3.2:10551205-10551224 | None:intergenic | 35.0% | |
| ATATGCTTACTCTTTGTCTG+AGG | + | chr3.2:10550798-10550817 | None:intergenic | 35.0% | |
| ATATGGCACACATCATACAA+TGG | + | chr3.2:10551009-10551028 | None:intergenic | 35.0% | |
| ATCTATTGCTGCCAATACTT+GGG | - | chr3.2:10550758-10550777 | MS.gene73089:intron | 35.0% | |
| ATTCAGTTTCTAAACCCCAA+AGG | - | chr3.2:10550594-10550613 | MS.gene73089:intron | 35.0% | |
| CAAGTCTCACAATCAAAATG+AGG | + | chr3.2:10551086-10551105 | None:intergenic | 35.0% | |
| CCTAATTGGTGAATGATGTT+GGG | + | chr3.2:10551204-10551223 | None:intergenic | 35.0% | |
| CTAATTGGTGAATGATGTTG+GGG | + | chr3.2:10551203-10551222 | None:intergenic | 35.0% | |
| TTGTATGATGTGTGCCATAT+TGG | - | chr3.2:10551009-10551028 | MS.gene73089:CDS | 35.0% | |
| AAATGTAGGGCCAGATAACT+TGG | - | chr3.2:10551303-10551322 | MS.gene73089:intron | 40.0% | |
| AGATACATGGATATCCGAAG+TGG | + | chr3.2:10550279-10550298 | None:intergenic | 40.0% | |
| ATATCCTGCAACTCTGCAAA+AGG | + | chr3.2:10551177-10551196 | None:intergenic | 40.0% | |
| CAACTGAAACCTTACCACTT+CGG | - | chr3.2:10550262-10550281 | MS.gene73089:intron | 40.0% | |
| CATCTATTGCTGCCAATACT+TGG | - | chr3.2:10550757-10550776 | MS.gene73089:intron | 40.0% | |
| CCCAACATCATTCACCAATT+AGG | - | chr3.2:10551201-10551220 | MS.gene73089:intron | 40.0% | |
| CTGAAGAGAATTGGAGTGAA+TGG | - | chr3.2:10550525-10550544 | MS.gene73089:intron | 40.0% | |
| GAGGAAAACAATGCATGGTA+TGG | + | chr3.2:10551067-10551086 | None:intergenic | 40.0% | |
| TATCCTGCAACTCTGCAAAA+GGG | + | chr3.2:10551176-10551195 | None:intergenic | 40.0% | |
| TGAGGAAGTCAACATCTTCA+AGG | - | chr3.2:10550560-10550579 | MS.gene73089:intron | 40.0% | |
| ! | CAAAGCACTCTGAAGAGAAT+TGG | - | chr3.2:10550516-10550535 | MS.gene73089:intron | 40.0% |
| !! | GAAAAGTTGATGAAGATGGC+CGG | - | chr3.2:10550043-10550062 | MS.gene73089:CDS | 40.0% |
| !! | GAAGATGTTGACTTCCTCAA+TGG | + | chr3.2:10550559-10550578 | None:intergenic | 40.0% |
| !! | GTGCTGTTTATTTACTCGTG+TGG | - | chr3.2:10550865-10550884 | MS.gene73089:intron | 40.0% |
| ACCGCTAACAACCCAAGTAT+TGG | + | chr3.2:10550772-10550791 | None:intergenic | 45.0% | |
| AGCTGAGCAATTTCAGAAGC+AGG | - | chr3.2:10551342-10551361 | MS.gene73089:intron | 45.0% | |
| CATGGATATCCGAAGTGGTA+AGG | + | chr3.2:10550274-10550293 | None:intergenic | 45.0% | |
| TCTGTTAGAACCGGTGGAAA+GGG | - | chr3.2:10550067-10550086 | MS.gene73089:CDS | 45.0% | |
| TTCTGTTAGAACCGGTGGAA+AGG | - | chr3.2:10550066-10550085 | MS.gene73089:CDS | 45.0% | |
| ! | GCCAATACTTGGGTTGTTAG+CGG | - | chr3.2:10550768-10550787 | MS.gene73089:intron | 45.0% |
| ! | TTTCCCTTTTGCAGAGTTGC+AGG | - | chr3.2:10551170-10551189 | MS.gene73089:intron | 45.0% |
| !! | ATGTTGACTTCCTCAATGGC+AGG | + | chr3.2:10550555-10550574 | None:intergenic | 45.0% |
| !! | TGTTGACTTCCTCAATGGCA+GGG | + | chr3.2:10550554-10550573 | None:intergenic | 45.0% |
| AGCAGTTTCAAAGGTCTCCC+CGG | + | chr3.2:10551437-10551456 | None:intergenic | 50.0% | |
| CGTCTGTAGTAGCTGTCTTG+TGG | + | chr3.2:10550485-10550504 | None:intergenic | 50.0% | |
| CTCCTCAGCAGCAGTTTCAA+AGG | + | chr3.2:10551446-10551465 | None:intergenic | 50.0% | |
| GAATGGTATCCCTGCCATTG+AGG | - | chr3.2:10550542-10550561 | MS.gene73089:intron | 50.0% | |
| TCTGACTGTACCCTTTCCAC+CGG | + | chr3.2:10550080-10550099 | None:intergenic | 50.0% | |
| TGATGCAACTGCAGCACAAG+AGG | - | chr3.2:10551381-10551400 | MS.gene73089:intron | 50.0% | |
| ! | ATGGCCGGTTCTGTTAGAAC+CGG | - | chr3.2:10550058-10550077 | MS.gene73089:CDS | 50.0% |
| ! | GTGGAAAGGGTACAGTCAGA+AGG | - | chr3.2:10550080-10550099 | MS.gene73089:CDS | 50.0% |
| !! | GACCTTTGAAACTGCTGCTG+AGG | - | chr3.2:10551441-10551460 | MS.gene73089:CDS | 50.0% |
| !! | TCCACCGGTTCTAACAGAAC+CGG | + | chr3.2:10550065-10550084 | None:intergenic | 50.0% |
| AACTGCTGCTGAGGAGACCA+AGG | - | chr3.2:10551450-10551469 | MS.gene73089:CDS | 55.0% | |
| CAGTTTCAAAGGTCTCCCCG+GGG | + | chr3.2:10551435-10551454 | None:intergenic | 55.0% | |
| CTGTACCAGATCTTGTCCCC+GGG | - | chr3.2:10551416-10551435 | MS.gene73089:CDS | 55.0% | |
| GCAGTTTCAAAGGTCTCCCC+GGG | + | chr3.2:10551436-10551455 | None:intergenic | 55.0% | |
| GCTGTACCAGATCTTGTCCC+CGG | - | chr3.2:10551415-10551434 | MS.gene73089:CDS | 55.0% | |
| GTTGCATCAGCTCCTGCTTC+AGG | + | chr3.2:10551370-10551389 | None:intergenic | 55.0% | |
| TGTACCAGATCTTGTCCCCG+GGG | - | chr3.2:10551417-10551436 | MS.gene73089:CDS | 55.0% | |
| CAGAAGCAGGCTCCTGAAGC+AGG | - | chr3.2:10551355-10551374 | MS.gene73089:intron | 60.0% | |
| ! | GCCGGTTCTGTTAGAACCGG+TGG | - | chr3.2:10550061-10550080 | MS.gene73089:CDS | 60.0% |
| GTCTCCCCGGGGACAAGATC+TGG | + | chr3.2:10551424-10551443 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 10550034 | 10551477 | 10550034 | ID=MS.gene73089 |
| chr3.2 | mRNA | 10550034 | 10551477 | 10550034 | ID=MS.gene73089.t1;Parent=MS.gene73089 |
| chr3.2 | exon | 10551410 | 10551477 | 10551410 | ID=MS.gene73089.t1.exon1;Parent=MS.gene73089.t1 |
| chr3.2 | CDS | 10551410 | 10551477 | 10551410 | ID=cds.MS.gene73089.t1;Parent=MS.gene73089.t1 |
| chr3.2 | exon | 10550896 | 10551044 | 10550896 | ID=MS.gene73089.t1.exon2;Parent=MS.gene73089.t1 |
| chr3.2 | CDS | 10550896 | 10551044 | 10550896 | ID=cds.MS.gene73089.t1;Parent=MS.gene73089.t1 |
| chr3.2 | exon | 10550704 | 10550760 | 10550704 | ID=MS.gene73089.t1.exon3;Parent=MS.gene73089.t1 |
| chr3.2 | CDS | 10550704 | 10550760 | 10550704 | ID=cds.MS.gene73089.t1;Parent=MS.gene73089.t1 |
| chr3.2 | exon | 10550289 | 10550327 | 10550289 | ID=MS.gene73089.t1.exon4;Parent=MS.gene73089.t1 |
| chr3.2 | CDS | 10550289 | 10550327 | 10550289 | ID=cds.MS.gene73089.t1;Parent=MS.gene73089.t1 |
| chr3.2 | exon | 10550034 | 10550200 | 10550034 | ID=MS.gene73089.t1.exon5;Parent=MS.gene73089.t1 |
| chr3.2 | CDS | 10550034 | 10550200 | 10550034 | ID=cds.MS.gene73089.t1;Parent=MS.gene73089.t1 |
| Gene Sequence |
| Protein sequence |
