AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003902

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003902.t1 MTR_4g066630 94.990 499 23 1 1 499 1 497 0.0 971
MS.gene003902.t1 MTR_4g066590 88.889 495 51 2 5 499 1 491 0.0 921
MS.gene003902.t1 MTR_4g066630 95.022 462 21 1 1 462 1 460 0.0 895
MS.gene003902.t1 MTR_4g066630 94.836 426 20 1 1 426 1 424 0.0 822
MS.gene003902.t1 MTR_4g066580 73.948 499 128 1 1 499 1 497 0.0 798
MS.gene003902.t1 MTR_3g028720 72.691 498 133 2 3 499 1 496 0.0 783
MS.gene003902.t1 MTR_4g067040 88.293 410 45 1 5 414 1 407 0.0 756
MS.gene003902.t1 MTR_3g028720 73.261 460 120 2 3 461 1 458 0.0 726
MS.gene003902.t1 MTR_4g066630 94.211 380 20 1 1 380 1 378 0.0 726
MS.gene003902.t1 MTR_2g098540 68.966 493 149 2 9 501 3 491 0.0 725
MS.gene003902.t1 MTR_4g067040 87.980 391 44 1 5 395 1 388 0.0 718
MS.gene003902.t1 MTR_2g098790 65.731 499 168 2 1 499 1 496 0.0 711
MS.gene003902.t1 MTR_4g066210 69.481 462 141 0 37 498 39 500 0.0 707
MS.gene003902.t1 MTR_2g098520 68.966 464 144 0 36 499 29 492 0.0 706
MS.gene003902.t1 MTR_6g078450 70.217 460 137 0 40 499 35 494 0.0 703
MS.gene003902.t1 MTR_4g066240 66.119 487 162 1 12 498 10 493 0.0 696
MS.gene003902.t1 MTR_4g066240 65.984 488 162 2 12 498 10 494 0.0 692
MS.gene003902.t1 MTR_4g066640 65.440 489 163 3 12 499 12 495 0.0 689
MS.gene003902.t1 MTR_2g098540 71.267 442 127 0 60 501 6 447 0.0 687
MS.gene003902.t1 MTR_4g066580 73.721 430 111 1 1 430 1 428 0.0 680
MS.gene003902.t1 MTR_6g078330 65.932 499 161 3 3 499 4 495 0.0 676
MS.gene003902.t1 MTR_6g078380 69.848 461 138 1 40 499 35 495 0.0 674
MS.gene003902.t1 MTR_3g028720 73.068 427 112 2 3 428 1 425 0.0 667
MS.gene003902.t1 MTR_6g078450 70.853 422 123 0 40 461 35 456 0.0 649
MS.gene003902.t1 MTR_5g071520 62.722 507 178 2 1 498 1 505 0.0 640
MS.gene003902.t1 MTR_3g026280 60.366 492 178 4 8 499 1 475 0.0 622
MS.gene003902.t1 MTR_4g066340 67.866 417 134 0 84 500 6 422 0.0 617
MS.gene003902.t1 MTR_4g066330 60.887 496 163 4 6 498 7 474 0.0 612
MS.gene003902.t1 MTR_3g028720 72.123 391 106 2 3 392 1 389 0.0 603
MS.gene003902.t1 MTR_4g066280 62.256 461 146 2 40 498 40 474 0.0 598
MS.gene003902.t1 MTR_4g066240 65.783 415 139 1 12 426 10 421 0.0 588
MS.gene003902.t1 MTR_4g023000 59.783 460 179 3 40 499 23 476 0.0 585
MS.gene003902.t1 MTR_6g078330 67.775 391 126 0 109 499 1 391 0.0 565
MS.gene003902.t1 MTR_3g026400 54.845 485 211 5 13 496 11 488 0.0 541
MS.gene003902.t1 MTR_2g043200 59.041 459 154 6 46 498 70 500 0.0 540
MS.gene003902.t1 MTR_4g015420 55.509 481 174 7 30 498 54 506 0.0 535
MS.gene003902.t1 MTR_4g066640 64.752 383 129 3 12 393 12 389 0.0 528
MS.gene003902.t1 MTR_6g078330 65.172 379 130 1 121 499 15 391 0.0 525
MS.gene003902.t1 MTR_6g078330 66.933 375 115 3 3 375 4 371 0.0 518
MS.gene003902.t1 MTR_3g026620 53.279 488 219 5 9 496 11 489 6.32e-179 516
MS.gene003902.t1 MTR_4g015460 55.011 469 173 6 41 499 42 482 6.35e-178 513
MS.gene003902.t1 MTR_3g026400 55.804 448 190 5 50 496 17 457 1.89e-176 508
MS.gene003902.t1 MTR_4g083000 50.000 494 228 4 14 498 1 484 7.54e-175 505
MS.gene003902.t1 MTR_5g044000 50.497 503 220 7 21 498 5 503 1.86e-173 502
MS.gene003902.t1 MTR_0110s0040 47.992 498 251 5 1 497 3 493 5.24e-165 480
MS.gene003902.t1 MTR_0110s0030 48.269 491 241 6 8 497 15 493 1.18e-163 477
MS.gene003902.t1 MTR_4g081530 48.785 494 242 6 40 527 23 511 3.25e-162 473
MS.gene003902.t1 MTR_3g033790 48.590 461 223 5 41 501 19 465 2.69e-156 457
MS.gene003902.t1 MTR_4g131810 49.780 454 224 3 46 496 33 485 1.04e-154 454
MS.gene003902.t1 MTR_3g033810 48.268 462 226 4 40 501 20 468 1.23e-154 453
MS.gene003902.t1 MTR_2g022760 49.889 449 221 3 50 496 45 491 3.99e-152 447
MS.gene003902.t1 MTR_4g131800 49.227 453 225 4 46 496 521 970 6.03e-146 448
MS.gene003902.t1 MTR_3g026400 56.369 369 153 5 13 380 11 372 2.68e-144 422
MS.gene003902.t1 MTR_2g079580 45.119 461 241 5 40 495 31 484 1.85e-142 423
MS.gene003902.t1 MTR_2g079550 45.652 460 238 5 41 495 30 482 6.42e-141 419
MS.gene003902.t1 MTR_2g022710 45.534 459 245 4 40 496 34 489 1.48e-139 416
MS.gene003902.t1 MTR_4g016920 47.300 463 227 6 40 500 26 473 3.56e-139 414
MS.gene003902.t1 MTR_4g083000 50.376 399 180 3 109 498 1 390 2.09e-138 409
MS.gene003902.t1 MTR_7g068910 43.086 499 259 6 1 498 1 475 5.09e-138 411
MS.gene003902.t1 MTR_5g068740 45.957 470 226 8 36 498 22 470 4.49e-137 409
MS.gene003902.t1 MTR_7g068910 42.240 509 259 7 1 498 1 485 8.85e-135 404
MS.gene003902.t1 MTR_1g088610 46.695 469 223 8 36 498 22 469 2.05e-134 406
MS.gene003902.t1 MTR_7g033860 44.516 465 229 7 43 498 50 494 1.94e-131 394
MS.gene003902.t1 MTR_0110s0040 48.421 380 192 3 1 380 3 378 1.39e-125 375
MS.gene003902.t1 MTR_0108s0030 42.325 456 234 4 43 498 5 431 1.53e-123 372
MS.gene003902.t1 MTR_2g079580 46.429 392 198 5 40 426 31 415 5.04e-123 369
MS.gene003902.t1 MTR_2g022760 47.692 390 200 3 109 496 1 388 3.84e-122 367
MS.gene003902.t1 MTR_7g051610 44.541 458 234 6 42 498 76 514 1.64e-121 370
MS.gene003902.t1 MTR_2g022710 44.575 424 230 4 40 461 34 454 4.53e-121 366
MS.gene003902.t1 MTR_2g022710 44.575 424 230 4 40 461 34 454 1.11e-120 365
MS.gene003902.t1 MTR_7g051510 43.545 457 240 5 42 498 76 514 3.99e-120 366
MS.gene003902.t1 MTR_2g022710 46.272 389 204 4 40 426 34 419 3.56e-118 357
MS.gene003902.t1 MTR_7g051650 43.137 459 240 6 42 498 76 515 1.27e-116 357
MS.gene003902.t1 MTR_7g051650 42.576 458 240 5 42 498 76 511 3.69e-115 353
MS.gene003902.t1 MTR_1g088630 44.494 445 196 10 60 498 14 413 4.16e-114 348
MS.gene003902.t1 MTR_2g022710 45.623 377 200 4 40 414 34 407 5.84e-112 341
MS.gene003902.t1 MTR_2g022710 43.590 390 216 3 109 496 1 388 2.66e-111 339
MS.gene003902.t1 MTR_4g016920 45.939 394 197 5 109 500 1 380 1.32e-110 338
MS.gene003902.t1 MTR_7g068900 43.596 406 196 6 104 498 17 400 2.24e-109 337
MS.gene003902.t1 MTR_1g088630 49.432 352 135 7 43 390 29 341 4.14e-108 329
MS.gene003902.t1 MTR_2g022710 49.068 322 161 3 40 361 34 352 9.47e-105 323
MS.gene003902.t1 MTR_7g056747 43.325 397 199 4 103 498 9 380 3.60e-100 310
MS.gene003902.t1 MTR_1g088630 53.160 269 114 4 43 307 29 289 1.34e-94 292
MS.gene003902.t1 MTR_4g016920 53.558 267 121 2 109 374 1 265 4.83e-94 291
MS.gene003902.t1 MTR_0110s0020 48.540 274 132 4 8 281 15 279 3.03e-89 279
MS.gene003902.t1 MTR_4g122980 27.033 418 226 16 105 516 183 527 3.92e-27 116
MS.gene003902.t1 MTR_8g038650 37.879 132 69 2 241 362 24 152 1.20e-22 95.1
MS.gene003902.t1 MTR_8g038650 55.556 54 23 1 50 103 7 59 1.90e-13 68.9
MS.gene003902.t1 MTR_5g069350 42.336 137 51 4 355 481 48 166 4.02e-19 85.9
MS.gene003902.t1 MTR_4g061170 72.500 40 9 1 462 499 22 61 1.11e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003902.t1 AT5G44640 55.444 496 209 5 5 499 1 485 0.0 579
MS.gene003902.t1 AT5G42260 55.242 496 210 5 5 499 1 485 0.0 575
MS.gene003902.t1 AT2G44450 57.391 460 191 2 41 500 31 485 0.0 572
MS.gene003902.t1 AT2G44480 56.304 460 200 1 40 499 65 523 0.0 560
MS.gene003902.t1 AT2G44480 56.304 460 200 1 40 499 34 492 0.0 558
MS.gene003902.t1 AT3G60130 56.087 460 196 3 41 499 30 484 0.0 554
MS.gene003902.t1 AT2G25630 53.112 482 203 4 18 498 7 466 0.0 540
MS.gene003902.t1 AT5G24550 53.548 465 211 5 41 501 32 495 1.21e-179 518
MS.gene003902.t1 AT5G24540 51.919 495 224 8 13 499 5 493 2.31e-173 503
MS.gene003902.t1 AT3G60140 48.861 483 236 6 20 499 12 486 2.30e-172 501
MS.gene003902.t1 AT1G51470 53.796 461 199 7 40 498 44 492 2.83e-172 499
MS.gene003902.t1 AT1G47600 53.362 461 201 7 40 498 44 492 3.80e-171 496
MS.gene003902.t1 AT1G47600 53.362 461 200 7 40 498 44 491 9.77e-171 495
MS.gene003902.t1 AT5G54570 50.318 471 217 4 40 501 29 491 3.43e-169 492
MS.gene003902.t1 AT3G18080 50.947 475 224 6 36 508 34 501 1.44e-168 489
MS.gene003902.t1 AT1G26560 50.972 463 217 6 41 498 32 489 2.66e-168 489
MS.gene003902.t1 AT2G44460 50.630 476 230 5 29 501 17 490 1.04e-165 484
MS.gene003902.t1 AT3G18070 49.787 470 217 8 32 498 21 474 3.76e-164 478
MS.gene003902.t1 AT3G60120 50.429 466 220 7 40 499 13 473 1.04e-163 478
MS.gene003902.t1 AT2G44470 47.558 471 243 4 31 498 19 488 1.21e-163 479
MS.gene003902.t1 AT3G18070 50.000 470 222 8 32 498 21 480 1.89e-163 476
MS.gene003902.t1 AT2G44490 50.212 472 228 6 33 499 7 476 1.85e-161 473
MS.gene003902.t1 AT2G44480 54.476 391 177 1 109 499 1 390 1.11e-160 466
MS.gene003902.t1 AT3G60130 54.523 398 175 3 103 499 40 432 3.94e-160 466
MS.gene003902.t1 AT5G25980 47.638 508 255 8 3 505 10 511 2.23e-159 467
MS.gene003902.t1 AT5G36890 49.458 461 220 4 42 501 17 465 8.33e-156 456
MS.gene003902.t1 AT5G36890 49.458 461 220 4 42 501 17 465 9.56e-156 456
MS.gene003902.t1 AT5G26000 47.505 501 242 10 13 500 3 495 5.98e-154 453
MS.gene003902.t1 AT5G24540 53.302 424 194 4 41 461 32 454 8.54e-154 449
MS.gene003902.t1 AT3G60130 53.518 398 168 4 103 499 40 421 5.62e-153 447
MS.gene003902.t1 AT5G54570 49.767 430 200 3 40 461 29 450 4.67e-152 445
MS.gene003902.t1 AT3G09260 48.387 465 233 6 41 501 35 496 1.95e-151 446
MS.gene003902.t1 AT2G32860 45.022 462 251 3 40 498 93 554 9.59e-149 442
MS.gene003902.t1 AT2G44470 47.235 434 226 3 31 462 19 451 1.60e-148 436
MS.gene003902.t1 AT2G32860 45.022 462 250 4 40 498 93 553 2.80e-148 441
MS.gene003902.t1 AT3G03640 44.800 500 263 6 3 496 1 493 1.11e-147 437
MS.gene003902.t1 AT3G21370 46.908 469 239 8 40 501 32 497 3.31e-147 435
MS.gene003902.t1 AT1G75940 48.017 479 219 12 41 501 37 503 4.16e-146 433
MS.gene003902.t1 AT1G02850 48.261 460 216 7 42 498 27 467 9.80e-146 431
MS.gene003902.t1 AT3G60140 48.900 409 199 5 20 426 12 412 9.45e-145 427
MS.gene003902.t1 AT5G25980 47.516 463 233 7 3 461 10 466 3.13e-144 426
MS.gene003902.t1 AT5G25980 47.516 463 233 7 3 461 10 466 3.13e-144 426
MS.gene003902.t1 AT1G52400 44.530 521 270 10 12 518 11 526 5.56e-144 427
MS.gene003902.t1 AT1G52400 44.530 521 270 10 12 518 11 526 5.56e-144 427
MS.gene003902.t1 AT5G28510 46.849 476 241 7 35 501 33 505 6.52e-144 427
MS.gene003902.t1 AT1G66280 45.951 494 253 9 16 501 9 496 5.17e-143 424
MS.gene003902.t1 AT1G61820 45.382 498 250 7 3 496 7 486 9.84e-142 421
MS.gene003902.t1 AT1G02850 46.170 483 215 8 42 498 27 490 1.22e-141 421
MS.gene003902.t1 AT1G02850 46.074 484 215 9 42 498 27 491 7.52e-140 416
MS.gene003902.t1 AT1G66270 46.581 468 238 8 40 501 35 496 1.30e-139 416
MS.gene003902.t1 AT5G26000 46.855 461 225 9 13 461 3 455 1.31e-138 411
MS.gene003902.t1 AT2G44480 60.256 312 122 2 40 351 65 374 1.22e-137 406
MS.gene003902.t1 AT4G21760 44.177 498 264 7 4 496 15 503 1.43e-137 411
MS.gene003902.t1 AT1G66270 46.368 468 237 9 40 501 35 494 1.75e-136 408
MS.gene003902.t1 AT5G54570 49.005 402 188 4 109 501 1 394 5.22e-136 404
MS.gene003902.t1 AT1G02850 46.739 460 196 8 42 498 27 440 2.92e-135 403
MS.gene003902.t1 AT4G27830 42.974 491 248 8 13 498 3 466 3.19e-134 401
MS.gene003902.t1 AT2G44450 53.687 339 152 2 162 500 1 334 1.62e-133 394
MS.gene003902.t1 AT1G61810 46.256 454 237 5 46 495 38 488 2.89e-133 400
MS.gene003902.t1 AT1G61810 46.256 454 237 5 46 495 38 488 3.76e-133 399
MS.gene003902.t1 AT1G60090 44.685 461 232 7 41 498 24 464 1.52e-132 397
MS.gene003902.t1 AT4G21760 43.373 498 258 8 4 496 15 493 2.17e-131 395
MS.gene003902.t1 AT4G22100 44.565 460 234 7 42 498 23 464 5.31e-129 388
MS.gene003902.t1 AT1G60090 43.617 470 231 9 41 498 24 471 1.34e-128 387
MS.gene003902.t1 AT2G44470 49.565 345 172 2 31 374 19 362 2.41e-127 380
MS.gene003902.t1 AT1G02850 48.418 411 173 7 42 426 27 424 7.19e-127 380
MS.gene003902.t1 AT1G52400 45.055 455 240 8 12 461 11 460 3.19e-126 379
MS.gene003902.t1 AT3G62750 40.351 513 274 7 30 539 12 495 1.61e-125 379
MS.gene003902.t1 AT4G27820 44.252 461 237 5 40 498 22 464 3.11e-125 378
MS.gene003902.t1 AT3G62750 40.351 513 274 7 30 539 38 521 5.05e-125 378
MS.gene003902.t1 AT1G45191 42.451 457 242 5 42 498 30 465 5.19e-121 367
MS.gene003902.t1 AT5G48375 45.414 447 172 9 37 480 34 411 8.82e-121 364
MS.gene003902.t1 AT3G18070 52.096 334 153 5 32 365 21 347 1.37e-120 362
MS.gene003902.t1 AT3G62740 41.880 468 248 8 33 498 15 460 6.92e-120 364
MS.gene003902.t1 AT1G45191 42.232 457 249 6 42 498 30 471 2.09e-118 361
MS.gene003902.t1 AT1G45191 41.991 462 248 7 42 498 30 476 1.09e-117 359
MS.gene003902.t1 AT1G45191 41.525 472 248 8 42 498 30 488 2.58e-116 356
MS.gene003902.t1 AT3G62750 41.781 438 224 6 30 464 38 447 7.37e-112 342
MS.gene003902.t1 AT3G62740 45.524 391 195 7 38 426 20 394 6.02e-111 338
MS.gene003902.t1 AT1G61810 45.385 390 206 5 41 426 33 419 1.15e-110 338
MS.gene003902.t1 AT1G60270 48.071 337 164 5 41 374 24 352 2.71e-108 330
MS.gene003902.t1 AT1G51490 48.857 350 160 7 48 393 27 361 5.46e-107 328
MS.gene003902.t1 AT3G62750 46.132 349 168 4 30 374 12 344 8.04e-107 326
MS.gene003902.t1 AT3G62750 46.132 349 168 4 30 374 12 344 8.04e-107 326
MS.gene003902.t1 AT3G62750 46.132 349 168 4 30 374 38 370 5.38e-106 326
MS.gene003902.t1 AT1G61810 44.501 391 210 5 109 495 1 388 7.56e-106 325
MS.gene003902.t1 AT1G45191 48.624 327 157 4 42 365 30 348 3.08e-105 323
MS.gene003902.t1 AT1G45191 48.624 327 157 4 42 365 30 348 4.62e-105 323
MS.gene003902.t1 AT2G44460 48.986 345 172 4 160 501 1 344 2.69e-104 322
MS.gene003902.t1 AT4G27830 41.985 393 210 5 109 498 1 378 1.95e-103 319
MS.gene003902.t1 AT2G44480 50.709 282 138 1 218 499 6 286 8.14e-99 303
MS.gene003902.t1 AT3G18070 47.937 315 156 5 187 498 94 403 1.63e-98 306
MS.gene003902.t1 AT3G18070 55.556 72 31 1 32 103 21 91 1.23e-18 89.0
MS.gene003902.t1 AT4G27820 42.347 392 211 4 109 498 1 379 1.91e-95 298
MS.gene003902.t1 AT4G27820 42.347 392 211 4 109 498 1 379 1.91e-95 298
MS.gene003902.t1 AT1G61820 45.087 346 184 4 155 496 4 347 4.02e-94 293
MS.gene003902.t1 AT3G62750 37.788 434 243 6 109 539 1 410 1.02e-92 291
MS.gene003902.t1 AT3G62750 37.788 434 243 6 109 539 1 410 1.02e-92 291
MS.gene003902.t1 AT3G18070 47.635 296 147 5 206 498 1 291 3.41e-91 284
MS.gene003902.t1 AT1G61810 51.111 270 130 2 41 310 33 300 6.63e-90 280
MS.gene003902.t1 AT1G60270 46.377 276 141 4 109 381 1 272 9.06e-79 251
MS.gene003902.t1 AT3G18070 47.541 244 124 3 187 428 94 335 7.00e-73 237
MS.gene003902.t1 AT3G18070 55.556 72 31 1 32 103 21 91 5.95e-19 89.0
MS.gene003902.t1 AT3G18070 47.321 224 114 3 206 427 1 222 5.50e-66 215
MS.gene003902.t1 AT5G16580 36.486 296 167 7 181 472 21 299 3.39e-48 170
MS.gene003902.t1 AT3G06510 25.359 418 233 16 104 495 159 523 1.58e-26 115
MS.gene003902.t1 AT3G06510 25.000 404 218 14 104 495 159 489 2.39e-26 114

Find 108 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCAGAAGGCTTCATATT+TGG 0.217301 4.4:+39591285 MS.gene003902:CDS
CTTGTTTAATTATAGGTTTA+TGG 0.235433 4.4:+39592535 MS.gene003902:intron
CACCAGGTCGATGTTCTTCT+TGG 0.235520 4.4:+39592109 MS.gene003902:CDS
AATTGCACCGGTGGTGATTC+TGG 0.263758 4.4:+39592141 MS.gene003902:CDS
ACCATCCATATTAACCATTA+TGG 0.266450 4.4:-39594209 None:intergenic
GAATCACCACCGGTGCAATT+TGG 0.269114 4.4:-39592138 None:intergenic
AATCATGAAGGATATGAATT+TGG 0.270686 4.4:+39591470 MS.gene003902:CDS
AAGTATCAAGTTTCTCAAAA+TGG 0.272356 4.4:+39592234 MS.gene003902:CDS
GTCCTCCATAATGGTTAATA+TGG 0.292343 4.4:+39594204 MS.gene003902:CDS
GTGGAAGAGGACCAAGTATA+TGG 0.292452 4.4:+39591352 MS.gene003902:CDS
CAATAACCTCATTGATGAAT+TGG 0.307366 4.4:+39591674 MS.gene003902:CDS
TGATAGTGTGCAGTTAAATA+AGG 0.310074 4.4:-39592171 None:intergenic
GAACTTTGCTTTAAAACATT+TGG 0.321295 4.4:+39592009 MS.gene003902:CDS
TGCAAATAGGTTCATTGAAT+AGG 0.340286 4.4:+39591256 MS.gene003902:CDS
AAGACAACAATAGAGAAAAT+AGG 0.342774 4.4:-39591173 None:intergenic
AGGGGTTATACTGTGAGATT+TGG 0.352676 4.4:+39593447 MS.gene003902:CDS
AACACTGCACCATTGCAAAT+AGG 0.356617 4.4:+39591243 MS.gene003902:CDS
GTGCCAAATATGAAGCCTTC+TGG 0.376589 4.4:-39591288 None:intergenic
CGAGCTACAGACTTTCAGTT+TGG 0.379816 4.4:+39592333 MS.gene003902:CDS
AAACTTACATTTAGACCAAT+TGG 0.381135 4.4:-39592883 None:intergenic
CCATGGAGTTACAGTCAAAA+TGG 0.382192 4.4:+39592066 MS.gene003902:CDS
TTAATTTATATTACTGAAAA+CGG 0.394477 4.4:+39593076 MS.gene003902:CDS
GTATAACATTGATCATTAAC+TGG 0.399683 4.4:+39592265 MS.gene003902:CDS
CAATCTCTAGAAGATGAATA+TGG 0.407604 4.4:+39591847 MS.gene003902:CDS
TATCTCATGCTTAATGTCAT+AGG 0.409360 4.4:-39591147 None:intergenic
AAATGTTGGGTCCTCCATAA+TGG 0.421142 4.4:+39594195 MS.gene003902:CDS
GATTGCTCTTTAGTAAACTT+TGG 0.424137 4.4:-39592617 None:intergenic
CTCAGCCTCATTGAAGGAAT+AGG 0.434784 4.4:-39594121 None:intergenic
GAAATTAGTACTATCAAAAC+AGG 0.439916 4.4:+39594253 MS.gene003902:CDS
TTATAGGTTTATGGATCCCT+TGG 0.446664 4.4:+39592544 MS.gene003902:intron
TGACTCGTATCATCGATATA+AGG 0.447189 4.4:+39591437 MS.gene003902:CDS
CCTTCCCTCAGCCTCATTGA+AGG 0.453743 4.4:-39594127 None:intergenic
CAGAGAATCTGTTAGGTAGC+TGG 0.461134 4.4:-39592732 None:intergenic
TATTCATCTTCTAGAGATTG+AGG 0.466417 4.4:-39591844 None:intergenic
GTTTCTCAAAATGGTTTGAT+CGG 0.472343 4.4:+39592243 MS.gene003902:CDS
GCACGAGATGGAAAACCAAT+TGG 0.474977 4.4:+39592868 MS.gene003902:CDS
GATGTTGAAACAGATGCCTT+GGG 0.476928 4.4:+39594456 MS.gene003902:intron
GAATTTGCATTGCTTAATTG+AGG 0.486109 4.4:-39592710 None:intergenic
GCAGCAAGTGAAGGTGGAAG+AGG 0.487766 4.4:+39591339 MS.gene003902:CDS
AACTAGACAGAGAATCTGTT+AGG 0.488098 4.4:-39592739 None:intergenic
ATACTTTGCATGGTCTCTGT+TGG 0.489827 4.4:+39593407 MS.gene003902:CDS
GAGTATTCTAGACCAAGATA+TGG 0.494023 4.4:-39591508 None:intergenic
CAGGGCTGGTGTAAATGTAA+AGG 0.496998 4.4:+39593383 MS.gene003902:intron
TGATGTTGAAACAGATGCCT+TGG 0.500108 4.4:+39594455 MS.gene003902:intron
AATTTCAGGTTGCTTCAGAA+TGG 0.500231 4.4:+39592987 MS.gene003902:intron
CTACAGACTTTCAGTTTGGA+TGG 0.501459 4.4:+39592337 MS.gene003902:CDS
AGTCAAAATGGATATGCAAA+TGG 0.504705 4.4:+39592078 MS.gene003902:CDS
GTGTGCAGTTAAATAAGGTT+CGG 0.508262 4.4:-39592166 None:intergenic
GATTCCTCAACTGATAATGA+TGG 0.508964 4.4:-39593204 None:intergenic
CATCGATATAAGGAAGATGT+TGG 0.511755 4.4:+39591447 MS.gene003902:CDS
CTGAGGGAAGGGACTATGAA+TGG 0.514749 4.4:+39594139 MS.gene003902:CDS
AGGGCTGGTGTAAATGTAAA+GGG 0.516329 4.4:+39593384 MS.gene003902:intron
CTATTCCTTCAATGAGGCTG+AGG 0.516412 4.4:+39594122 MS.gene003902:CDS
TGGATAATCTCCATTGGCCA+AGG 0.518626 4.4:-39592561 None:intergenic
ATGCTGGTAAATCAAATGTT+GGG 0.521252 4.4:+39594182 MS.gene003902:CDS
TCCATAATGGTTAATATGGA+TGG 0.523669 4.4:+39594208 MS.gene003902:CDS
GCAAATGGCGCAATGGCACC+AGG 0.525181 4.4:+39592093 MS.gene003902:CDS
CTTCAATGAGGCTGAGGGAA+GGG 0.525219 4.4:+39594128 MS.gene003902:CDS
TTCAATGAACCTATTTGCAA+TGG 0.527927 4.4:-39591252 None:intergenic
TTCATCATTACTTTAACAGA+AGG 0.528077 4.4:+39591207 MS.gene003902:CDS
TGGAAGAGGACCAAGTATAT+GGG 0.531156 4.4:+39591353 MS.gene003902:CDS
AATGCTGGTAAATCAAATGT+TGG 0.538243 4.4:+39594181 MS.gene003902:CDS
ATTGATGAATTGGTGGCAAA+TGG 0.539277 4.4:+39591684 MS.gene003902:CDS
TAACCTCATTGATGAATTGG+TGG 0.539436 4.4:+39591677 MS.gene003902:CDS
TGATCTTACCAATGCTCGCA+TGG 0.539778 4.4:-39592588 None:intergenic
TCTTCTGCATATCAATATGA+AGG 0.549189 4.4:+39591315 MS.gene003902:CDS
TTTATGGATCCCTTGGCCAA+TGG 0.549813 4.4:+39592551 MS.gene003902:CDS
GTTCAGAATGTTGCAAATGC+TGG 0.555235 4.4:+39594166 MS.gene003902:CDS
GGATAACTTTGAACGAGCCA+TGG 0.555448 4.4:+39592049 MS.gene003902:CDS
TTGGAGATAGAGTAAAACAT+TGG 0.559088 4.4:+39592028 MS.gene003902:CDS
GTGATTGCAGTCGCACGAGA+TGG 0.559174 4.4:+39592856 MS.gene003902:intron
TATCCTCAAAAGATCAGTGA+TGG 0.559832 4.4:+39591393 MS.gene003902:CDS
GTGAAAGTATCCCATATACT+TGG 0.569026 4.4:-39591363 None:intergenic
TTGCCACCAATTCATCAATG+AGG 0.575333 4.4:-39591680 None:intergenic
AAGATCAGTGATGGAAACAA+TGG 0.577375 4.4:+39591402 MS.gene003902:CDS
GAGCAATCTAAGCAAGTCAA+AGG 0.581133 4.4:+39592632 MS.gene003902:CDS
GGATAATCTCCATTGGCCAA+GGG 0.583402 4.4:-39592560 None:intergenic
TGGATATGCAAATGGCGCAA+TGG 0.586330 4.4:+39592086 MS.gene003902:CDS
GGAAGATGTTGGAATCATGA+AGG 0.590169 4.4:+39591458 MS.gene003902:CDS
TGGTTGAATCCAAATTGCAC+CGG 0.592901 4.4:+39592129 MS.gene003902:CDS
CGATCCATCATTATCAGTTG+AGG 0.593498 4.4:+39593200 MS.gene003902:CDS
AGTGGTGGAATTAATCAAGA+AGG 0.596031 4.4:+39591639 MS.gene003902:CDS
GAAGGTGCAGCAAGTGAAGG+TGG 0.597164 4.4:+39591333 MS.gene003902:CDS
CCTTCAATGAGGCTGAGGGA+AGG 0.597841 4.4:+39594127 MS.gene003902:CDS
AGGATCTTGAAGACACATGT+TGG 0.598388 4.4:-39594101 None:intergenic
CTTTAAGATAAAAGAGATGA+CGG 0.600540 4.4:-39593260 None:intergenic
TATGAAGGTGCAGCAAGTGA+AGG 0.604375 4.4:+39591330 MS.gene003902:CDS
CTTCTTCAGTTCAATCCCCA+AGG 0.607731 4.4:-39594472 None:intergenic
GCAGAAGGAAAACTAAGTGG+TGG 0.613240 4.4:+39591624 MS.gene003902:intron
AAGATCCTATTCCTTCAATG+AGG 0.617508 4.4:+39594116 MS.gene003902:CDS
TTCGGTTCCAGAATCACCAC+CGG 0.620495 4.4:-39592148 None:intergenic
TGGTTGTATGTTTATCCAAG+AGG 0.620982 4.4:+39593007 MS.gene003902:CDS
TTATGTTTGTTATCTGAGAG+CGG 0.625110 4.4:-39592294 None:intergenic
ATGTAAAGGGATACTTTGCA+TGG 0.626161 4.4:+39593397 MS.gene003902:CDS
ATCCATATTAACCATTATGG+AGG 0.631731 4.4:-39594206 None:intergenic
TATGCAGAAGGAAAACTAAG+TGG 0.632774 4.4:+39591621 MS.gene003902:intron
TATTCCTTCAATGAGGCTGA+GGG 0.633853 4.4:+39594123 MS.gene003902:CDS
GAATGCTTAGTTGAGCAACA+AGG 0.651866 4.4:-39594279 None:intergenic
CTCAACTAAGCATTCTCACG+AGG 0.652339 4.4:+39594287 MS.gene003902:CDS
TGTATCACTCACATGATGAG+AGG 0.655919 4.4:-39591883 None:intergenic
CAAACATAATCAAAATGCTG+CGG 0.660707 4.4:+39592308 MS.gene003902:CDS
TTGAATCCAAATTGCACCGG+TGG 0.672179 4.4:+39592132 MS.gene003902:CDS
AACCAAGAAGAACATCGACC+TGG 0.677559 4.4:-39592111 None:intergenic
TCTCTAGAAGATGAATATGG+CGG 0.701636 4.4:+39591850 MS.gene003902:CDS
ATGTTGAAACAGATGCCTTG+GGG 0.705649 4.4:+39594457 MS.gene003902:intron
TGGAAACAATGGAGACGTTG+CGG 0.708998 4.4:+39591413 MS.gene003902:CDS
TATGGATGGTCGTATCAATG+CGG 0.767395 4.4:+39594222 MS.gene003902:CDS
AAAAGACTTTCAAAACTACG+CGG 0.803991 4.4:+39591987 MS.gene003902:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAAATAAAAAATA+TGG - chr4.4:39591596-39591615 None:intergenic 0.0%
!! AAAAAAATAAAAAATATGGT+TGG - chr4.4:39591592-39591611 None:intergenic 10.0%
!! AGTATTATAAATTTAAATGA+TGG + chr4.4:39592817-39592836 MS.gene003902:intron 10.0%
!! TTAATTTATATTACTGAAAA+CGG + chr4.4:39593076-39593095 MS.gene003902:CDS 10.0%
!!! AATTTAGAATAATATACTTT+AGG - chr4.4:39593910-39593929 None:intergenic 10.0%
!!! TTTTCAGTAATATAAATTAA+AGG - chr4.4:39593076-39593095 None:intergenic 10.0%
!! TTCATTGTTTAAATATAAGA+AGG + chr4.4:39593652-39593671 MS.gene003902:intron 15.0%
!!! ACTTATATTTAGTTTAATAG+TGG - chr4.4:39593992-39594011 None:intergenic 15.0%
!!! TTATATGTGTATTTAATTTC+AGG + chr4.4:39592973-39592992 MS.gene003902:intron 15.0%
!!! TTTTTTATAATTTATGCAGA+AGG + chr4.4:39591609-39591628 MS.gene003902:intron 15.0%
!! ACCTATAATTAAACAAGAAA+TGG - chr4.4:39592532-39592551 None:intergenic 20.0%
!! CTTGTTTAATTATAGGTTTA+TGG + chr4.4:39592535-39592554 MS.gene003902:intron 20.0%
!! TAGATATGATTGTGTTAATT+TGG - chr4.4:39592465-39592484 None:intergenic 20.0%
!! TCTTATGTAAAAAAAATTGC+AGG + chr4.4:39591784-39591803 MS.gene003902:intron 20.0%
!! TGATTACAAAAAATCTACTA+GGG + chr4.4:39593809-39593828 MS.gene003902:intron 20.0%
!! TTTAATTGAGAATAGTCAAT+TGG - chr4.4:39591554-39591573 None:intergenic 20.0%
!!! AAAGGTTCATTTGATTTTAT+TGG + chr4.4:39592650-39592669 MS.gene003902:CDS 20.0%
!!! AATATGGTTTATACCTTTTA+AGG + chr4.4:39593873-39593892 MS.gene003902:intron 20.0%
!!! ATTTGTAACTCTTTTTCATT+GGG + chr4.4:39591816-39591835 MS.gene003902:CDS 20.0%
!!! ATTTTCATAGATTACAAAGA+TGG + chr4.4:39593474-39593493 MS.gene003902:CDS 20.0%
!!! GCAATTTTTTTTACATAAGA+CGG - chr4.4:39591784-39591803 None:intergenic 20.0%
!!! GGAATTATTTTTTCCTTAAA+AGG - chr4.4:39593889-39593908 None:intergenic 20.0%
!!! TAATTCAAATTTTCATGTGT+AGG + chr4.4:39593162-39593181 MS.gene003902:intron 20.0%
!!! TCCATTTCTTGTTTAATTAT+AGG + chr4.4:39592528-39592547 MS.gene003902:intron 20.0%
!!! TTTTGATAGTACTAATTTCA+TGG - chr4.4:39594252-39594271 None:intergenic 20.0%
! AAAAATTCTTCAGTTTGTGA+AGG - chr4.4:39593772-39593791 None:intergenic 25.0%
! AAAATTCTTCAGTTTGTGAA+GGG - chr4.4:39593771-39593790 None:intergenic 25.0%
! AAACTTACATTTAGACCAAT+TGG - chr4.4:39592886-39592905 None:intergenic 25.0%
! AAGACAACAATAGAGAAAAT+AGG - chr4.4:39591176-39591195 None:intergenic 25.0%
! AAGTATCAAGTTTCTCAAAA+TGG + chr4.4:39592234-39592253 MS.gene003902:CDS 25.0%
! AATCATGAAGGATATGAATT+TGG + chr4.4:39591470-39591489 MS.gene003902:CDS 25.0%
! ATTTCTTTCAATGAAATGCA+GGG + chr4.4:39593365-39593384 MS.gene003902:intron 25.0%
! CAATGAAAAAGAGTTACAAA+TGG - chr4.4:39591817-39591836 None:intergenic 25.0%
! GAAATTAGTACTATCAAAAC+AGG + chr4.4:39594253-39594272 MS.gene003902:CDS 25.0%
! GTATAACATTGATCATTAAC+TGG + chr4.4:39592265-39592284 MS.gene003902:CDS 25.0%
! GTGATTACAAAAAATCTACT+AGG + chr4.4:39593808-39593827 MS.gene003902:intron 25.0%
! TAATCACAACTTGGTTAAAA+TGG - chr4.4:39593795-39593814 None:intergenic 25.0%
! TATTTCTTTCAATGAAATGC+AGG + chr4.4:39593364-39593383 MS.gene003902:intron 25.0%
! TTCATCATTACTTTAACAGA+AGG + chr4.4:39591207-39591226 MS.gene003902:CDS 25.0%
!! AATTTTGTAGCACTGTTAAA+TGG - chr4.4:39593580-39593599 None:intergenic 25.0%
!! CATATAGATTTTCCATATCT+TGG + chr4.4:39591496-39591515 MS.gene003902:CDS 25.0%
!! GGTATAAAATTGACTTACTT+GGG - chr4.4:39591533-39591552 None:intergenic 25.0%
!! TATGATTGTGTTAATTTGGA+TGG - chr4.4:39592461-39592480 None:intergenic 25.0%
!! TCATCTCTTTTATCTTAAAG+AGG + chr4.4:39593263-39593282 MS.gene003902:CDS 25.0%
!! TGGTATAAAATTGACTTACT+TGG - chr4.4:39591534-39591553 None:intergenic 25.0%
!!! AGTCTATTTCATTTTTTGCA+TGG + chr4.4:39593323-39593342 MS.gene003902:intron 25.0%
!!! ATTTTGAGAAACTTGATACT+TGG - chr4.4:39592235-39592254 None:intergenic 25.0%
!!! CATTTGTAACTCTTTTTCAT+TGG + chr4.4:39591815-39591834 MS.gene003902:CDS 25.0%
!!! CTTTAAGATAAAAGAGATGA+CGG - chr4.4:39593263-39593282 None:intergenic 25.0%
!!! GAACTTTGCTTTAAAACATT+TGG + chr4.4:39592009-39592028 MS.gene003902:CDS 25.0%
!!! GATTTTTTGTAATCACAACT+TGG - chr4.4:39593804-39593823 None:intergenic 25.0%
!!! GCAACATCTTACTTTTTTTT+TGG + chr4.4:39592920-39592939 MS.gene003902:intron 25.0%
AAAAGACTTTCAAAACTACG+CGG + chr4.4:39591987-39592006 MS.gene003902:CDS 30.0%
AAAAGATATCAAAAGCTCTC+AGG + chr4.4:39593501-39593520 MS.gene003902:intron 30.0%
AAATATAAGTTCGACAGATG+AGG + chr4.4:39594002-39594021 MS.gene003902:intron 30.0%
AACATAAGAACAACTTGACA+TGG - chr4.4:39594384-39594403 None:intergenic 30.0%
AATATAAGTTCGACAGATGA+GGG + chr4.4:39594003-39594022 MS.gene003902:intron 30.0%
AATGCTGGTAAATCAAATGT+TGG + chr4.4:39594181-39594200 MS.gene003902:CDS 30.0%
ACCATCCATATTAACCATTA+TGG - chr4.4:39594212-39594231 None:intergenic 30.0%
AGTCAAAATGGATATGCAAA+TGG + chr4.4:39592078-39592097 MS.gene003902:CDS 30.0%
ATCCATATTAACCATTATGG+AGG - chr4.4:39594209-39594228 None:intergenic 30.0%
ATGCTGGTAAATCAAATGTT+GGG + chr4.4:39594182-39594201 MS.gene003902:CDS 30.0%
CAAACATAATCAAAATGCTG+CGG + chr4.4:39592308-39592327 MS.gene003902:CDS 30.0%
CAAATGAAAATGATATGGGT+GGG + chr4.4:39594412-39594431 MS.gene003902:intron 30.0%
CAATAACCTCATTGATGAAT+TGG + chr4.4:39591674-39591693 MS.gene003902:CDS 30.0%
GAATTTGCATTGCTTAATTG+AGG - chr4.4:39592713-39592732 None:intergenic 30.0%
GATTGCTCTTTAGTAAACTT+TGG - chr4.4:39592620-39592639 None:intergenic 30.0%
GTTTCTCAAAATGGTTTGAT+CGG + chr4.4:39592243-39592262 MS.gene003902:CDS 30.0%
TCAAACATGAATATACCATC+AGG + chr4.4:39593842-39593861 MS.gene003902:intron 30.0%
TCCATAATGGTTAATATGGA+TGG + chr4.4:39594208-39594227 MS.gene003902:CDS 30.0%
TCTTCTGCATATCAATATGA+AGG + chr4.4:39591315-39591334 MS.gene003902:CDS 30.0%
TGCAAATAGGTTCATTGAAT+AGG + chr4.4:39591256-39591275 MS.gene003902:CDS 30.0%
TGTGTTTACAACAACAACTA+TGG - chr4.4:39593614-39593633 None:intergenic 30.0%
TTAATCTCGAATAACCTTTG+AGG + chr4.4:39594034-39594053 MS.gene003902:intron 30.0%
TTCAATGAACCTATTTGCAA+TGG - chr4.4:39591255-39591274 None:intergenic 30.0%
TTGGAGATAGAGTAAAACAT+TGG + chr4.4:39592028-39592047 MS.gene003902:CDS 30.0%
! AATAGGAATGATTTTCCAGA+AGG + chr4.4:39591273-39591292 MS.gene003902:CDS 30.0%
! ACCCATATCATTTTCATTTG+CGG - chr4.4:39594412-39594431 None:intergenic 30.0%
! CAATCTCTAGAAGATGAATA+TGG + chr4.4:39591847-39591866 MS.gene003902:CDS 30.0%
! CTCTGTTGGATAATTTTGAA+TGG + chr4.4:39593421-39593440 MS.gene003902:CDS 30.0%
! TATTCATCTTCTAGAGATTG+AGG - chr4.4:39591847-39591866 None:intergenic 30.0%
! TGATAGTGTGCAGTTAAATA+AGG - chr4.4:39592174-39592193 None:intergenic 30.0%
! TTGAAAGTCTTTTCTGTAGT+TGG - chr4.4:39591980-39591999 None:intergenic 30.0%
! TTTACATAAGACGGCTATTT+TGG - chr4.4:39591775-39591794 None:intergenic 30.0%
!! TTATGTTTGTTATCTGAGAG+CGG - chr4.4:39592297-39592316 None:intergenic 30.0%
AAAAAGTCTCGAATTCCTCT+TGG - chr4.4:39593025-39593044 None:intergenic 35.0%
AACTAGACAGAGAATCTGTT+AGG - chr4.4:39592742-39592761 None:intergenic 35.0%
AAGATCCTATTCCTTCAATG+AGG + chr4.4:39594116-39594135 MS.gene003902:CDS 35.0%
ACTACTCCTATGCTTACAAA+TGG + chr4.4:39594348-39594367 MS.gene003902:intron 35.0%
ATGTAAAGGGATACTTTGCA+TGG + chr4.4:39593397-39593416 MS.gene003902:CDS 35.0%
CATCGATATAAGGAAGATGT+TGG + chr4.4:39591447-39591466 MS.gene003902:CDS 35.0%
GAGTATTCTAGACCAAGATA+TGG - chr4.4:39591511-39591530 None:intergenic 35.0%
GCAAATGAAAATGATATGGG+TGG + chr4.4:39594411-39594430 MS.gene003902:intron 35.0%
GCCGCAAATGAAAATGATAT+GGG + chr4.4:39594408-39594427 MS.gene003902:intron 35.0%
GTCCTCCATAATGGTTAATA+TGG + chr4.4:39594204-39594223 MS.gene003902:CDS 35.0%
GTGTGCAGTTAAATAAGGTT+CGG - chr4.4:39592169-39592188 None:intergenic 35.0%
TAACCTCATTGATGAATTGG+TGG + chr4.4:39591677-39591696 MS.gene003902:CDS 35.0%
TATCCTCAAAAGATCAGTGA+TGG + chr4.4:39591393-39591412 MS.gene003902:CDS 35.0%
TATGCAGAAGGAAAACTAAG+TGG + chr4.4:39591621-39591640 MS.gene003902:intron 35.0%
TGACTCGTATCATCGATATA+AGG + chr4.4:39591437-39591456 MS.gene003902:CDS 35.0%
TGGTTGTATGTTTATCCAAG+AGG + chr4.4:39593007-39593026 MS.gene003902:CDS 35.0%
TTATAGGTTTATGGATCCCT+TGG + chr4.4:39592544-39592563 MS.gene003902:intron 35.0%
TTTCCAGAAGGCTTCATATT+TGG + chr4.4:39591285-39591304 MS.gene003902:CDS 35.0%
! AAAGTCTTTTCTGTAGTTGG+AGG - chr4.4:39591977-39591996 None:intergenic 35.0%
! AAGATCAGTGATGGAAACAA+TGG + chr4.4:39591402-39591421 MS.gene003902:CDS 35.0%
! AGTGGTGGAATTAATCAAGA+AGG + chr4.4:39591639-39591658 MS.gene003902:CDS 35.0%
! ATTGATGAATTGGTGGCAAA+TGG + chr4.4:39591684-39591703 MS.gene003902:CDS 35.0%
! GATAATTTTGAATGGCACAG+GGG + chr4.4:39593429-39593448 MS.gene003902:CDS 35.0%
! GATTCCTCAACTGATAATGA+TGG - chr4.4:39593207-39593226 None:intergenic 35.0%
! GATTGTTTTCCTCAGGTTTT+AGG - chr4.4:39593967-39593986 None:intergenic 35.0%
! GGATAATTTTGAATGGCACA+GGG + chr4.4:39593428-39593447 MS.gene003902:CDS 35.0%
! TCAAGTTGTTCTTATGTTGC+TGG + chr4.4:39594386-39594405 MS.gene003902:intron 35.0%
! TCTCTAGAAGATGAATATGG+CGG + chr4.4:39591850-39591869 MS.gene003902:CDS 35.0%
! TGGATAATTTTGAATGGCAC+AGG + chr4.4:39593427-39593446 MS.gene003902:CDS 35.0%
! TTTCCATCACTGATCTTTTG+AGG - chr4.4:39591399-39591418 None:intergenic 35.0%
!! AATTTCAGGTTGCTTCAGAA+TGG + chr4.4:39592987-39593006 MS.gene003902:intron 35.0%
!! GGTTTTTGGATAATCTCCAT+TGG - chr4.4:39592570-39592589 None:intergenic 35.0%
!! GTGAAAGTATCCCATATACT+TGG - chr4.4:39591366-39591385 None:intergenic 35.0%
AAATGTTGGGTCCTCCATAA+TGG + chr4.4:39594195-39594214 MS.gene003902:CDS 40.0%
AACACTGCACCATTGCAAAT+AGG + chr4.4:39591243-39591262 MS.gene003902:CDS 40.0%
AGCATAGGAGTAGTAGCATA+TGG - chr4.4:39594342-39594361 None:intergenic 40.0%
AGGATCTTGAAGACACATGT+TGG - chr4.4:39594104-39594123 None:intergenic 40.0%
AGGGGTTATACTGTGAGATT+TGG + chr4.4:39593447-39593466 MS.gene003902:CDS 40.0%
AGGTTGTAAGTCGTTTCAAG+AGG - chr4.4:39593947-39593966 None:intergenic 40.0%
ATACTTTGCATGGTCTCTGT+TGG + chr4.4:39593407-39593426 MS.gene003902:CDS 40.0%
ATCAAAAGCTCTCAGGATTG+TGG + chr4.4:39593508-39593527 MS.gene003902:intron 40.0%
ATGTGTTGCCACATGTGATA+AGG + chr4.4:39594059-39594078 MS.gene003902:intron 40.0%
ATGTTGAAACAGATGCCTTG+GGG + chr4.4:39594457-39594476 MS.gene003902:intron 40.0%
CCATCAGGCATCTCAAAATA+TGG + chr4.4:39593857-39593876 MS.gene003902:intron 40.0%
CCATGGAGTTACAGTCAAAA+TGG + chr4.4:39592066-39592085 MS.gene003902:CDS 40.0%
CGATCCATCATTATCAGTTG+AGG + chr4.4:39593200-39593219 MS.gene003902:CDS 40.0%
CTACAGACTTTCAGTTTGGA+TGG + chr4.4:39592337-39592356 MS.gene003902:CDS 40.0%
GAATGCTTAGTTGAGCAACA+AGG - chr4.4:39594282-39594301 None:intergenic 40.0%
GACTTACAACCTAAAACCTG+AGG + chr4.4:39593955-39593974 MS.gene003902:intron 40.0%
GAGCAATCTAAGCAAGTCAA+AGG + chr4.4:39592632-39592651 MS.gene003902:CDS 40.0%
GATGTTGAAACAGATGCCTT+GGG + chr4.4:39594456-39594475 MS.gene003902:intron 40.0%
GGAAGATGTTGGAATCATGA+AGG + chr4.4:39591458-39591477 MS.gene003902:CDS 40.0%
GGCCGCAAATGAAAATGATA+TGG + chr4.4:39594407-39594426 MS.gene003902:intron 40.0%
GTTCAGAATGTTGCAAATGC+TGG + chr4.4:39594166-39594185 MS.gene003902:CDS 40.0%
TATGGATGGTCGTATCAATG+CGG + chr4.4:39594222-39594241 MS.gene003902:CDS 40.0%
TATTCCTTCAATGAGGCTGA+GGG + chr4.4:39594123-39594142 MS.gene003902:CDS 40.0%
TGATGTTGAAACAGATGCCT+TGG + chr4.4:39594455-39594474 MS.gene003902:intron 40.0%
TGCACACCATTTGTAAGCAT+AGG - chr4.4:39594357-39594376 None:intergenic 40.0%
TGGAAGAGGACCAAGTATAT+GGG + chr4.4:39591353-39591372 MS.gene003902:CDS 40.0%
TGGTTGAATCCAAATTGCAC+CGG + chr4.4:39592129-39592148 MS.gene003902:CDS 40.0%
TGTATCACTCACATGATGAG+AGG - chr4.4:39591886-39591905 None:intergenic 40.0%
TTGCCACCAATTCATCAATG+AGG - chr4.4:39591683-39591702 None:intergenic 40.0%
! AGGGCTGGTGTAAATGTAAA+GGG + chr4.4:39593384-39593403 MS.gene003902:intron 40.0%
! TTTCTTTTCCATCTAGCGCT+TGG + chr4.4:39592397-39592416 MS.gene003902:intron 40.0%
!!! CCATATTTTGAGATGCCTGA+TGG - chr4.4:39593860-39593879 None:intergenic 40.0%
!!! CCATTTTGACTGTAACTCCA+TGG - chr4.4:39592069-39592088 None:intergenic 40.0%
AACCAAGAAGAACATCGACC+TGG - chr4.4:39592114-39592133 None:intergenic 45.0%
CAGAGAATCTGTTAGGTAGC+TGG - chr4.4:39592735-39592754 None:intergenic 45.0%
CCACATGTGATAAGGCGTAT+AGG + chr4.4:39594067-39594086 MS.gene003902:intron 45.0%
CCTATACGCCTTATCACATG+TGG - chr4.4:39594070-39594089 None:intergenic 45.0%
CGAGCTACAGACTTTCAGTT+TGG + chr4.4:39592333-39592352 MS.gene003902:CDS 45.0%
CTATTCCTTCAATGAGGCTG+AGG + chr4.4:39594122-39594141 MS.gene003902:CDS 45.0%
CTCAACTAAGCATTCTCACG+AGG + chr4.4:39594287-39594306 MS.gene003902:CDS 45.0%
CTCAGCCTCATTGAAGGAAT+AGG - chr4.4:39594124-39594143 None:intergenic 45.0%
CTTCTTCAGTTCAATCCCCA+AGG - chr4.4:39594475-39594494 None:intergenic 45.0%
CTTTCAATGAAATGCAGGGC+TGG + chr4.4:39593369-39593388 MS.gene003902:intron 45.0%
GCACGAGATGGAAAACCAAT+TGG + chr4.4:39592868-39592887 MS.gene003902:CDS 45.0%
GCAGAAGGAAAACTAAGTGG+TGG + chr4.4:39591624-39591643 MS.gene003902:intron 45.0%
GGATAACTTTGAACGAGCCA+TGG + chr4.4:39592049-39592068 MS.gene003902:CDS 45.0%
GGATAATCTCCATTGGCCAA+GGG - chr4.4:39592563-39592582 None:intergenic 45.0%
GTGCCAAATATGAAGCCTTC+TGG - chr4.4:39591291-39591310 None:intergenic 45.0%
GTGGAAGAGGACCAAGTATA+TGG + chr4.4:39591352-39591371 MS.gene003902:CDS 45.0%
TCCAAAAACCATGCGAGCAT+TGG + chr4.4:39592580-39592599 MS.gene003902:CDS 45.0%
TGATCTTACCAATGCTCGCA+TGG - chr4.4:39592591-39592610 None:intergenic 45.0%
TGGAAACAATGGAGACGTTG+CGG + chr4.4:39591413-39591432 MS.gene003902:CDS 45.0%
TGGATAATCTCCATTGGCCA+AGG - chr4.4:39592564-39592583 None:intergenic 45.0%
TGGATATGCAAATGGCGCAA+TGG + chr4.4:39592086-39592105 MS.gene003902:CDS 45.0%
TTGAATCCAAATTGCACCGG+TGG + chr4.4:39592132-39592151 MS.gene003902:CDS 45.0%
TTTATGGATCCCTTGGCCAA+TGG + chr4.4:39592551-39592570 MS.gene003902:CDS 45.0%
! ACCAATGCTCGCATGGTTTT+TGG - chr4.4:39592584-39592603 None:intergenic 45.0%
! AGTGGCAGATTGTTTTCCTC+AGG - chr4.4:39593974-39593993 None:intergenic 45.0%
! CAGGGCTGGTGTAAATGTAA+AGG + chr4.4:39593383-39593402 MS.gene003902:intron 45.0%
! TATGAAGGTGCAGCAAGTGA+AGG + chr4.4:39591330-39591349 MS.gene003902:CDS 45.0%
AGACCCAAGCAAGGCTTACT+TGG - chr4.4:39594313-39594332 None:intergenic 50.0%
AGGCCAAGTAAGCCTTGCTT+GGG + chr4.4:39594307-39594326 MS.gene003902:intron 50.0%
CACCAGGTCGATGTTCTTCT+TGG + chr4.4:39592109-39592128 MS.gene003902:CDS 50.0%
CTGAGGGAAGGGACTATGAA+TGG + chr4.4:39594139-39594158 MS.gene003902:CDS 50.0%
CTTCAATGAGGCTGAGGGAA+GGG + chr4.4:39594128-39594147 MS.gene003902:CDS 50.0%
GAATCACCACCGGTGCAATT+TGG - chr4.4:39592141-39592160 None:intergenic 50.0%
GTGGCAACACATCTCCTCAA+AGG - chr4.4:39594051-39594070 None:intergenic 50.0%
TTCGGTTCCAGAATCACCAC+CGG - chr4.4:39592151-39592170 None:intergenic 50.0%
! AATTGCACCGGTGGTGATTC+TGG + chr4.4:39592141-39592160 MS.gene003902:CDS 50.0%
! AGTGACGCAGTCAGAAGTGT+AGG - chr4.4:39592426-39592445 None:intergenic 50.0%
! TGGAAGCATAGACCCAAGCA+AGG - chr4.4:39594322-39594341 None:intergenic 50.0%
CCTTCAATGAGGCTGAGGGA+AGG + chr4.4:39594127-39594146 MS.gene003902:CDS 55.0%
CCTTCCCTCAGCCTCATTGA+AGG - chr4.4:39594130-39594149 None:intergenic 55.0%
GAGGCCAAGTAAGCCTTGCT+TGG + chr4.4:39594306-39594325 MS.gene003902:intron 55.0%
GCAGCAAGTGAAGGTGGAAG+AGG + chr4.4:39591339-39591358 MS.gene003902:CDS 55.0%
GTAGGCTACCAAGCGCTAGA+TGG - chr4.4:39592408-39592427 None:intergenic 55.0%
GTGATTGCAGTCGCACGAGA+TGG + chr4.4:39592856-39592875 MS.gene003902:intron 55.0%
! GAAGGTGCAGCAAGTGAAGG+TGG + chr4.4:39591333-39591352 MS.gene003902:CDS 55.0%
GCAAATGGCGCAATGGCACC+AGG + chr4.4:39592093-39592112 MS.gene003902:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 39591150 39594524 39591150 ID=MS.gene003902
chr4.4 mRNA 39591150 39594524 39591150 ID=MS.gene003902.t1;Parent=MS.gene003902
chr4.4 exon 39591150 39591534 39591150 ID=MS.gene003902.t1.exon1;Parent=MS.gene003902.t1
chr4.4 CDS 39591150 39591534 39591150 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39591628 39591705 39591628 ID=MS.gene003902.t1.exon2;Parent=MS.gene003902.t1
chr4.4 CDS 39591628 39591705 39591628 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39591806 39591893 39591806 ID=MS.gene003902.t1.exon3;Parent=MS.gene003902.t1
chr4.4 CDS 39591806 39591893 39591806 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39591987 39592358 39591987 ID=MS.gene003902.t1.exon4;Parent=MS.gene003902.t1
chr4.4 CDS 39591987 39592358 39591987 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39592550 39592767 39592550 ID=MS.gene003902.t1.exon5;Parent=MS.gene003902.t1
chr4.4 CDS 39592550 39592767 39592550 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39592866 39592897 39592866 ID=MS.gene003902.t1.exon6;Parent=MS.gene003902.t1
chr4.4 CDS 39592866 39592897 39592866 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39592995 39593097 39592995 ID=MS.gene003902.t1.exon7;Parent=MS.gene003902.t1
chr4.4 CDS 39592995 39593097 39592995 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39593184 39593292 39593184 ID=MS.gene003902.t1.exon8;Parent=MS.gene003902.t1
chr4.4 CDS 39593184 39593292 39593184 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39593386 39593495 39593386 ID=MS.gene003902.t1.exon9;Parent=MS.gene003902.t1
chr4.4 CDS 39593386 39593495 39593386 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39594089 39594313 39594089 ID=MS.gene003902.t1.exon10;Parent=MS.gene003902.t1
chr4.4 CDS 39594089 39594313 39594089 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
chr4.4 exon 39594469 39594524 39594469 ID=MS.gene003902.t1.exon11;Parent=MS.gene003902.t1
chr4.4 CDS 39594469 39594524 39594469 ID=cds.MS.gene003902.t1;Parent=MS.gene003902.t1
Gene Sequence

>MS.gene003902

ATGACATTAAGCATGAGATATCTCCTATTTTCTCTATTGTTGTCTTTAGCTTCAACATTCATCATTACTTTAACAGAAGGTCAAGAAATAATTAACACTGCACCATTGCAAATAGGTTCATTGAATAGGAATGATTTTCCAGAAGGCTTCATATTTGGCACAGCATCTTCTGCATATCAATATGAAGGTGCAGCAAGTGAAGGTGGAAGAGGACCAAGTATATGGGATACTTTCACTCATAGATATCCTCAAAAGATCAGTGATGGAAACAATGGAGACGTTGCGGTTGACTCGTATCATCGATATAAGGAAGATGTTGGAATCATGAAGGATATGAATTTGGATGCATATAGATTTTCCATATCTTGGTCTAGAATACTCCCAAGTAAGTCAATTTTATACCAATTGACTATTCTCAATTAAACTTAGTTTCTTTTCACCAACCATATTTTTTATTTTTTTTTTATAATTTATGCAGAAGGAAAACTAAGTGGTGGAATTAATCAAGAAGGAATTGACTATTACAATAACCTCATTGATGAATTGGTGGCAAATGGTAAGAATACAAACTTCATTTTCAATTATTGTAGTATTATTATTTTTATTTGCATTTTAGCATTTTCCAAAATAGCCGTCTTATGTAAAAAAAATTGCAGGTTTACAACCATTTGTAACTCTTTTTCATTGGGATCTTCCTCAATCTCTAGAAGATGAATATGGCGGTTTCTTAAGTCCTCTCATCATGTGAGTGATACAATTTCAAATTTTAATCTGAAAATTTATTTTATATAGTTATGAAAATGTTTCTTATTCATCTTTTCATACCTCCAACTACAGAAAAGACTTTCAAAACTACGCGGAACTTTGCTTTAAAACATTTGGAGATAGAGTAAAACATTGGATAACTTTGAACGAGCCATGGAGTTACAGTCAAAATGGATATGCAAATGGCGCAATGGCACCAGGTCGATGTTCTTCTTGGTTGAATCCAAATTGCACCGGTGGTGATTCTGGAACCGAACCTTATTTAACTGCACACTATCAACTTCTAGCACATGCAGCAGCTGTTAATGTCTACAAGACCAAGTATCAAGTTTCTCAAAATGGTTTGATCGGTATAACATTGATCATTAACTGGAATGTGCCGCTCTCAGATAACAAACATAATCAAAATGCTGCGGAACGAGCTACAGACTTTCAGTTTGGATGGTAAGTACAAAGATAAAGCTATTTTCACTGCAAACTTGTTTCTTTTCCATCTAGCGCTTGGTAGCCTACACTTCTGACTGCGTCACTTACATAATTCTACCATCCAAATTAACACAATCATATCTATCTATCTCTCTCTCTCTCTATATATATACACATGATAACTTATTCCATTTCTTGTTTAATTATAGGTTTATGGATCCCTTGGCCAATGGAGATTATCCAAAAACCATGCGAGCATTGGTAAGATCACGATTACCAAAGTTTACTAAAGAGCAATCTAAGCAAGTCAAAGGTTCATTTGATTTTATTGGCATAAACTACTACTCTTCTTGCTATGTTTCTGATGCACCTCAATTAAGCAATGCAAATTCCAGCTACCTAACAGATTCTCTGTCTAGTTTTTCATGTAAGCACTTTTAGCTTACTGCTATCTTGTATTTATTGATTGTTTAATTAGTATTATAAATTTAAATGATGGTATATTTTATATTTTTGTGATTGCAGTCGCACGAGATGGAAAACCAATTGGTCTAAATGTAAGTTTATACGTTAAAAATTGCAACATCTTACTTTTTTTTTGGTTTTCTGTTATTGTGTAATTAATAATCTTGTTATATGTGTATTTAATTTCAGGTTGCTTCAGAATGGTTGTATGTTTATCCAAGAGGAATTCGAGACTTTTTGATATACGCTAAAGAAAAATACAACAATCCTTTAATTTATATTACTGAAAACGGTACGCAAATCGTGCATGTATCATATTGAAACATATTTTCGTTATATAACGAAACTATGTATGATAATTCAAATTTTCATGTGTAGGTATAAATGAGTACGACGATCCATCATTATCAGTTGAGGAATCTCTTTTAGATATTTATAGAATTGATTATCATTACCGTCATCTCTTTTATCTTAAAGAGGCAATCATGTAAGTAATATATTGAAATATCTAAAGAGTAGTCTATTTCATTTTTTGCATGGAATTTAACTATATGATATTATTTCTTTCAATGAAATGCAGGGCTGGTGTAAATGTAAAGGGATACTTTGCATGGTCTCTGTTGGATAATTTTGAATGGCACAGGGGTTATACTGTGAGATTTGGAATGATTTTCATAGATTACAAAGATGGTTTGAAAAGATATCAAAAGCTCTCAGGATTGTGGTTCAAGAATTTTCTCACATCAGAGAACAAAAAAGTTTACATAGATTCCATTTAACAGTGCTACAAAATTCTTTATAAACACCATAGTTGTTGTTGTAAACACAATAATTTCATTTAATTATTTCATTGTTTAAATATAAGAAGGATACTATTTGTAATTGTATGTAAATATATTATTATTATTGATATTATTTGATGATTCTACATATATCTTGTAATTTCATTTTGCATGACTAGTCCCTTCACAAACTGAAGAATTTTTCCATTTTAACCAAGTTGTGATTACAAAAAATCTACTAGGGTATGTTTATGTCAAACATGAATATACCATCAGGCATCTCAAAATATGGTTTATACCTTTTAAGGAAAAAATAATTCCTAAAGTATATTATTCTAAATTGAAAATATTCTAGTCCTCTTGAAACGACTTACAACCTAAAACCTGAGGAAAACAATCTGCCACTATTAAACTAAATATAAGTTCGACAGATGAGGGATTGACTCTTAATCTCGAATAACCTTTGAGGAGATGTGTTGCCACATGTGATAAGGCGTATAGGAACAACTGCTACCAACATGTGTCTTCAAGATCCTATTCCTTCAATGAGGCTGAGGGAAGGGACTATGAATGGTGCAGTTCAGAATGTTGCAAATGCTGGTAAATCAAATGTTGGGTCCTCCATAATGGTTAATATGGATGGTCGTATCAATGCGGTGCACCATGAAATTAGTACTATCAAAACAGGTATCCTTGTTGCTCAACTAAGCATTCTCACGAGGCCAAGTAAGCCTTGCTTGGGTCTATGCTTCCATATGCTACTACTCCTATGCTTACAAATGGTGTGCAATAGCCATGTCAAGTTGTTCTTATGTTGCTGGCCGCAAATGAAAATGATATGGGTGGGAATAATTCTCTTGCTGCTATTGATGTTGAAACAGATGCCTTGGGGATTGAACTGAAGAAGCATAAGTGCATTAATGTTGTTAAAGACTAG
Protein sequence

>MS.gene003902.t1

MTLSMRYLLFSLLLSLASTFIITLTEGQEIINTAPLQIGSLNRNDFPEGFIFGTASSAYQYEGAASEGGRGPSIWDTFTHRYPQKISDGNNGDVAVDSYHRYKEDVGIMKDMNLDAYRFSISWSRILPKGKLSGGINQEGIDYYNNLIDELVANGLQPFVTLFHWDLPQSLEDEYGGFLSPLIIKDFQNYAELCFKTFGDRVKHWITLNEPWSYSQNGYANGAMAPGRCSSWLNPNCTGGDSGTEPYLTAHYQLLAHAAAVNVYKTKYQVSQNGLIGITLIINWNVPLSDNKHNQNAAERATDFQFGWFMDPLANGDYPKTMRALVRSRLPKFTKEQSKQVKGSFDFIGINYYSSCYVSDAPQLSNANSSYLTDSLSSFSFARDGKPIGLNVASEWLYVYPRGIRDFLIYAKEKYNNPLIYITENGINEYDDPSLSVEESLLDIYRIDYHYRHLFYLKEAIMAGVNVKGYFAWSLLDNFEWHRGYTVRFGMIFIDYKDGTTATNMCLQDPIPSMRLREGTMNGAVQNVANAGKSNVGSSIMVNMDGRINAVHHEISTIKTGILVAQLSILTRPNALGIELKKHKCINVVKD